MD03G1006900.v1.1

Mitochondrial protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000106
Physical Location & Seq
Forward (+)
560520 .. 572151
11632 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1006900.v1.1.491

Sequence Viewer

Length: 2679 bp
ATGTGCTCAACATTTTCCCTCGCTCGCAGCAAAGGCATACACGAGAGACAGCAATGGAAAATTCCCGCCCCGAAAATATATGGTCATAGCAACAAGAATCCGGAATTATCAATACAAGTCATTCCCTCTGTTGCTGATTTACAAGTACAAGATATCATTCAAAGGCAGTCCCAGACATGTAAGATGGCCAAAGAAGGCGGCCGCAGAAAACCAGGGTTGGATCTTAGCTTTGTTGAAGAATCATTTGAAAGATGTAGGAAGATTTGTGTTAAAGGCACATTATTGATGACAAAGGAGCGCCGAAAAGCATTATGGGCTATCTATGGAGGACATGAACTTGTGGATGGCCCCAATTCTGTCCACATGAGCTCGGACGTTCTTGATATGTGGGAACCATTTAGAGATGTAATCGAGGGAATGAGAATGGACACAAGAAAATTTCGCTATCAGAATTTTCAAGAGCTCTATATTTACAGCTACCGCGTGGTGGGGACTTTTGGCTTAATGAGTGTATTGTCTAAATACCAACATGGTATCAGAGCCAATCTGATCAAAGAGTGGGGCGTTTTTTTATTCTTAAAAAGGGGGAAAATTCTAAGTGAAACCCTAGAATCAAAATTTTTCAAATTCATGGCTGGTTCAAGTAGCAGTGGTGAGATTCGAACTCCTATCTTCAGTGGAGTGAACTACGACTTCTGGAGGACAAAGTTGAGAACAATTTTTGTATCTCATGATTTGTGGAGCTTGATTGAAGATGGCTATATTGTACCTCAAAGTATTGTGGTTTTGACAGAGACTCAAACAAGGGAGCTGAGGGAAAACATCAAGAGAGATGCAAAAGCTCTTGGGGTCATCCAAAATGCTATCTCAGATGAAATTTTTCCGAGAATCTCAAATGAGACAAGTGCCAAATCAGCTTGGGATGTGCTGGAGAAAGTCTACAAAGACTCTACTAAGGTAAGGGTTGTCAAACTCCAATCTTTGAGAAGAGATTTTGAGTATATAAGAATGAAAGATGATGAATTGCTAGATGATTACCTGAGTAGATTGTCTGAGATTATTAATCAAATGAAAAGCTATGGTGAAATCTTGTCTGATGAGAGAATAGTTCAGAAGTATCTAATTAGCTTGCCAAGAAAGTATGACAACATAGTTTCAATCATAGAGGAGACTAAGGATTTGTCAACTTTGAGTGTTGAAGAATTGATTGGTTCTCTCAAAGGTTTTGCTCAGAGACTATCTAGACATGACAACAATGATGTAGAAAATGCTTTCCAAACACTTACAATGAATCCAAAAACTCAAGCTAGTTCAAGTCAAAGCAAAGCCAAGTCTAACAAGAATTGGAAAGGCAAAAACAAAGTTTGGGAAGGAAAGGGTGATTTTGAAGAGAAGAAAAAGAATTTGATAAGTTTTGTCAAGAATCAAGATATTGGCTTGGAAAGACAACTTACAGTCAGCTACACTCCACAACAAAATGGAGTAGCTGAGAGAAAAAACAGAACCATAGTCGAAATGGCTAAGGCAATGTTACATGACAAGGGAATACTTCAATATTTGTGGGGTGAGGCAGTTAATACAGCGGTCTACTTGATGAATAGGCATCCAACAGTGGCAATTGAAGATAAGACACTATTTGAGGCATGGAGTGGAAGAAAGCCTTCCGTGAATCATTTGAGAGTGTTTGGGTCAATCTGCTTTGCTTATGTTCCGAAAGAACTAAGACAAAAACTTGATGGGTCAAGTGAAAGATGCATTTTCATAGGCTATGCTTCATACACAAAAGGGTACAGACTCTACAATTTAAAAAGGAAGAAAGTTGTGGTTTGTAGAGATGTTCTTTTTAGTGAGAAATCTTCGTGGGATTGGAATCAAGCAACCATCCGAGAGGAATCTGCACTAATTAGAATTGAAGAAGAAAATCAACCAAATGAGGAAGAAATTGAGCCAGAAATTCCACAATTATCACCTGAAAACAGTCATCAAGTTCACTCTCCTACACCTTCAAGTCCAAGTTCAACACCAGTTCGGTTAAGGAGCTTAAATGAAGTCTATGAAAGCTGCAATTTCTGTGTTGAAGAACCAGAAAACTTTGATGATGCAATGAAAGAAGAGACATGGAAGGCTGCAATGCAAGAGAAGATCAATGTCATTGTGAAAAACAAGACATGGGAACTAGTGGACAGACCTTGTGACAACTCCGTCATTGGAGTGAAGTGGATTTATAAGACTAAGCTCAATCAAGATGGTTCTGTTCAAAGGAACAAAGCTAGATTGGTAGCAAAGGGTTATTCTCAGAAACCCGGGATTGATTTCCAAGAAACATTTGCATCGAGTGTTCCTGTTCTAGGAATTGCACCAGATTCTCTGATTTCTGCTCAAGGTGTATATGAAGGCGCAAATCATTTTGGCACTGCAAATCAATTGACCAACATCCTTAGAGACGTTGGGGAGGAAGTTTATCTTCCCCAAGACGAGCTTGCGCATTTTGGGTTATCCGATACCGATATTTTTGCAAGAAAGGTAACTGATCAGTGGAGAGAGTTCATGAAAGAACAGATTGCAAGTGCTAGGATATATTTCAACCTGATATGGTCAGCGTTATTCTTGTATCGTGGCATCTTGGATGCTATTGAAGATAACGGCTATGATAACTTGACAAAGAGAGCATATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4219 RVT_2 Retrotran_gag_2 gag_pre-integrs rve zf-CCHC

Protein Analysis

893

Amino Acids

102.68

Weight (kDa)

8.55

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQS_PSY PF00494 131 - 171 2.5e-07 Squalene/phytoene synthase
Retrotran_gag_2 PF14223 238 - 412 8.3e-33 gag-polypeptide of LTR copia-type
SH3_retrovirus PF25597 564 - 626 2.6e-22 Retroviral polymerase SH3-like domain
RVT_2 PF07727 721 - 779 5.4e-14 Reverse transcriptase (RNA-dependent DNA polymerase)
SQS_PSY PF00494 794 - 865 1.3e-13 Squalene/phytoene synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000106)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20980 AT3G21000
fragaria_vesca FvH4_1g15931 FvH4_1g15931 FvH4_1g18381 FvH4_1g18382 FvH4_1g22571 FvH4_1g24950 FvH4_1g29372 FvH4_2g03582 FvH4_2g09851 FvH4_2g13112 FvH4_2g20843 FvH4_2g22701 FvH4_2g22702 FvH4_2g23403 FvH4_2g40201 FvH4_3g03542 FvH4_3g03543 FvH4_3g04761 FvH4_3g10464 FvH4_3g12741 FvH4_3g15781 FvH4_3g30981 FvH4_4g00985 FvH4_4g07035 FvH4_4g08192 FvH4_4g08211 FvH4_4g08553 FvH4_4g14242 FvH4_4g14243 FvH4_4g14641 FvH4_4g14843 FvH4_4g14844 FvH4_4g15061 FvH4_4g16281 FvH4_4g19152 FvH4_4g26451 FvH4_5g06442 FvH4_5g22521 FvH4_5g24301 FvH4_5g38641 FvH4_6g02393 FvH4_6g12782 FvH4_6g13871 FvH4_6g19391 FvH4_6g20105 FvH4_6g21292 FvH4_6g23581 FvH4_6g29153 FvH4_6g29691 FvH4_6g30871 FvH4_6g33351 FvH4_6g37042 FvH4_6g38781 FvH4_6g40340 FvH4_7g11221 FvH4_7g23352 FvH4_7g23352 FvH4_7g31212 FvH4_7g32172 FvH4_7g32173 FvH4_7g33701
malus_domestica MD03G1006900.v1.1 MD04G1039100.v1.1 MD04G1239100.v1.1 MD05G1048700.v1.1 MD08G1001800.v1.1 MD09G1165800.v1.1 MD11G1065200.v1.1 MD11G1138200.v1.1 MD14G1033300.v1.1 MD14G1173900.v1.1 MD15G1218200.v1.1 MD16G1034000.v1.1
prunus_persica Prupe.1G219500_v2.0.a1 Prupe.2G169400_v2.0.a1 Prupe.4G109700_v2.0.a1 Prupe.7G022000_v2.0.a1
pyrus_communis pycom01g02000 pycom02g13350 pycom03g01010 pycom04g05000 pycom04g06100 pycom05g05580 pycom05g09950 pycom05g13280 pycom05g26610 pycom06g05940 pycom06g07760 pycom07g06120 pycom07g24780 pycom08g08820 pycom09g03600 pycom09g07200 pycom10g01280 pycom10g06730 pycom10g08410 pycom10g25520 pycom11g00470 pycom11g05250 pycom11g25180 pycom12g08050 pycom13g14760 pycom13g28170 pycom15g14310 pycom15g19880 pycom15g24230 pycom15g33640 pycom16g02150 pycom16g16240 pycom16g20050 pycom16g21090 pycom17g06100
rosa_chinensis RchiOBHm_Chr1g0322161 RchiOBHm_Chr1g0326341 RchiOBHm_Chr1g0357701 RchiOBHm_Chr1g0379861 RchiOBHm_Chr1g0383421 RchiOBHm_Chr2g0093981 RchiOBHm_Chr2g0139051 RchiOBHm_Chr2g0145501 RchiOBHm_Chr3g0450971 RchiOBHm_Chr3g0451441 RchiOBHm_Chr3g0457411 RchiOBHm_Chr4g0436261 RchiOBHm_Chr5g0051431 RchiOBHm_Chr5g0064031 RchiOBHm_Chr5g0073141 RchiOBHm_Chr5g0080061 RchiOBHm_Chr7g0200561 RchiOBHm_Chr7g0235911
rosa_laevigata RLG00000000145 RLG00000000429 RLG00000020339 RLG00000035212
rosa_multiflora Rmu_co7977988.1_g000001 Rmu_co8037866.1_g000001 Rmu_co8243895.1_g000001 Rmu_co8363787.1_g000001 Rmu_co8381003.1_g000001 Rmu_sc0000263.1_g000006 Rmu_sc0000349.1_g000002 Rmu_sc0000455.1_g000031 Rmu_sc0000936.1_g000022 Rmu_sc0001035.1_g000023 Rmu_sc0001169.1_g000019 Rmu_sc0001250.1_g000026 Rmu_sc0002200.1_g000051 Rmu_sc0002230.1_g000027 Rmu_sc0002591.1_g000039 Rmu_sc0003182.1_g000003 Rmu_sc0003441.1_g000068 Rmu_sc0003722.1_g000018 Rmu_sc0003764.1_g000007 Rmu_sc0006014.1_g000016 Rmu_sc0012582.1_g000002 Rmu_sc0013519.1_g000002 Rmu_sc0014550.1_g000005 Rmu_sc0014857.1_g000007 Rmu_sc0019855.1_g000001 Rmu_sc0030839.1_g000001 Rmu_sc0031308.1_g000001
rosa_roxburghii Rroxscaffold_1G00011290 Rroxscaffold_1G00041340 Rroxscaffold_1G00053780 Rroxscaffold_1G00059130 Rroxscaffold_1G00061580 Rroxscaffold_2G00080940 Rroxscaffold_2G00109540 Rroxscaffold_3G00228400 Rroxscaffold_3G00237710 Rroxscaffold_4G00285210 Rroxscaffold_4G00299600 Rroxscaffold_4G00324940 Rroxscaffold_4G00330580 Rroxscaffold_5G00333000 Rroxscaffold_5G00347920 Rroxscaffold_5G00375310 Rroxscaffold_6G00394760 Rroxscaffold_6G00406260 Rroxscaffold_7G00165690 Rroxscaffold_7G00187680
rosa_rugosa Rorug01G0050200 Rorug02G0273800 Rorug06G0490600 Rorug06G0490600 Rorug06G0490700
rosa_samantha Rh1AG348400 Rh5AG222600 Rh6DG000500 Rh6DG194500 Rh7BG452700
rosa_wichuraiana Rw0G003060 Rw0G010530 Rw0G012970 Rw0G022560 Rw0G023390 Rw1G001270 Rw1G001530 Rw1G006070 Rw1G008210 Rw1G022090 Rw1G023320 Rw2G003790 Rw2G004140 Rw2G005280 Rw2G006460 Rw2G019620 Rw2G019840 Rw2G022530 Rw2G023280 Rw2G024990 Rw2G042380 Rw2G051390 Rw3G001640 Rw3G016720 Rw3G017100 Rw3G020890 Rw3G020900 Rw3G021100 Rw4G004820 Rw4G014950 Rw4G030920 Rw4G031400 Rw5G008500 Rw5G017950 Rw5G020170 Rw5G030160 Rw5G033050 Rw5G034640 Rw5G043920 Rw6G005060 Rw6G005540 Rw6G006720 Rw6G008460 Rw6G008670 Rw6G014780 Rw6G019100 Rw6G019350 Rw6G026310 Rw7G016810 Rw7G019180 Rw7G022890 Rw7G025880 Rw7G026390 Rw7G028340 Rw7G034850 Rw7G035170 Rw7G035420 Rw7G037990 Rw7G041560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2226
AasI GACNNNNNNGTC 1 cut(s) 2201
Acc16I TGCGCA 1 cut(s) 2484
AccI GTMKAC 2 cut(s) 939, 1587
AccII CGCG 1 cut(s) 483
AccIII TCCGGA 1 cut(s) 100
AciI CCGC 5 cut(s) 66, 198, 202, 481, 1583
AclWI GGATC 1 cut(s) 228
AcoI YGGCCR 2 cut(s) 186, 199
AcsI RAATTY 9 cut(s) 60, 437, 451, 591, 617, 626, 876, 1402, 1953
AcuI CTGAAG 1 cut(s) 658
AfaI GTAC 3 cut(s) 147, 768, 1790
AfiI CCNNNNNNNGG 2 cut(s) 487, 2348
AflIII ACRYGT 1 cut(s) 176
AhlI ACTAGT 1 cut(s) 2176
AjnI CCWGG 1 cut(s) 211
AjuI GAANNNNNNNTTGG 2 cut(s) 1191, 1223
Alw21I GWGCWC 3 cut(s) 8, 371, 465
Alw26I GTCTC 7 cut(s) 40, 788, 893, 1163, 1228, 2108, 2436
AlwI GGATC 1 cut(s) 228
Ama87I CYCGRG 1 cut(s) 2303
Aor13HI TCCGGA 1 cut(s) 100
AoxI GGCC 3 cut(s) 186, 199, 346
ApeKI GCWGC 3 cut(s) 27, 2061, 2126
ApoI RAATTY 9 cut(s) 60, 437, 451, 591, 617, 626, 876, 1402, 1953
ArsI GACNNNNNNTTYG 2 cut(s) 1162, 1194
AseI ATTAAT 1 cut(s) 1062
Asp700I GAANNNNTTC 2 cut(s) 879, 1660
AspLEI GCGC 3 cut(s) 300, 2399, 2485
AspS9I GGNCC 1 cut(s) 347
AsuC2I CCSGG 2 cut(s) 2304, 2305
AsuHPI GGTGA 5 cut(s) 665, 1094, 1391, 1577, 1959
AsuII TTCGAA 1 cut(s) 661
AvaI CYCGRG 1 cut(s) 2303
BalI TGGCCA 1 cut(s) 188
BanII GRGCYC 2 cut(s) 371, 465
BauI CACGAG 1 cut(s) 41
Bbv12I GWGCWC 3 cut(s) 8, 371, 465
BbvCI CCTCAGC 1 cut(s) 812
BbvI GCAGC 3 cut(s) 39, 2048, 2113
BccI CCATC 6 cut(s) 178, 338, 749, 1730, 1889, 2240
BceAI ACGGC 1 cut(s) 2659
BcgI CGANNNNNNTGC 2 cut(s) 2495, 2529
BciT130I CCWGG 1 cut(s) 213
BclI TGATCA 2 cut(s) 549, 2530
BcnI CCSGG 2 cut(s) 2304, 2305
BcoDI GTCTC 7 cut(s) 40, 788, 893, 1163, 1228, 2108, 2436
BcuI ACTAGT 1 cut(s) 2176
BfaI CTAG 8 cut(s) 608, 1028, 1242, 1308, 2177, 2271, 2348, 2571
BfoI RGCGCY 1 cut(s) 301
BisI GCNGC 5 cut(s) 28, 199, 202, 2062, 2127
BlsI GCNGC 5 cut(s) 29, 200, 203, 2063, 2128
Bme1390I CCNGG 3 cut(s) 213, 2304, 2305
BmeT110I CYCGRG 1 cut(s) 2303
BmgT120I GGNCC 1 cut(s) 347
BmiI GGNNCC 2 cut(s) 349, 393
BmrFI CCNGG 3 cut(s) 213, 2304, 2305
BmsI GCATC 7 cut(s) 823, 1612, 1742, 2089, 2339, 2617, 2628
BpmI CTGGAG 2 cut(s) 718, 950
Bpu10I CCTNAGC 2 cut(s) 812, 1521
Bpu14I TTCGAA 1 cut(s) 661
BpuEI CTTGAG 2 cut(s) 1287, 2364
BpuMI CCSGG 2 cut(s) 2304, 2305
BsaBI GATNNNNATC 1 cut(s) 1869
BsaJI CCNNGG 2 cut(s) 212, 2303
BsaWI WCCGGW 1 cut(s) 100
Bsc4I CCNNNNNNNGG 2 cut(s) 487, 2348
Bse1I ACTGG 1 cut(s) 2024
Bse3DI GCAATG 4 cut(s) 59, 1533, 2109, 2136
Bse8I GATNNNNATC 1 cut(s) 1869
BseAI TCCGGA 1 cut(s) 100
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 2 cut(s) 212, 2303
BseGI GGATG 7 cut(s) 349, 852, 928, 1603, 1881, 2433, 2632
BseJI GATNNNNATC 1 cut(s) 1869
BseLI CCNNNNNNNGG 2 cut(s) 487, 2348
BseMI GCAATG 4 cut(s) 59, 1533, 2109, 2136
BseMII CTCAG 7 cut(s) 803, 882, 1031, 1044, 1244, 1479, 2309
BseNI ACTGG 1 cut(s) 2024
BseRI GAGGAG 1 cut(s) 1181
BseX3I CGGCCG 1 cut(s) 199
BseXI GCAGC 3 cut(s) 39, 2048, 2113
BsgI GTGCAG 1 cut(s) 1881
Bsh1236I CGCG 1 cut(s) 483
Bsh1285I CGRYCG 1 cut(s) 202
BshFI GGCC 3 cut(s) 188, 201, 348
BsiEI CGRYCG 1 cut(s) 202
BsiHKAI GWGCWC 3 cut(s) 8, 371, 465
BsiHKCI CYCGRG 1 cut(s) 2303
BsiSI CCGG 2 cut(s) 101, 2304
BslFI GGGAC 2 cut(s) 154, 505
BslI CCNNNNNNNGG 2 cut(s) 487, 2348
BsmAI GTCTC 7 cut(s) 40, 788, 893, 1163, 1228, 2108, 2436
BsmBI CGTCTC 1 cut(s) 2436
BsmFI GGGAC 2 cut(s) 154, 505
BsnI GGCC 3 cut(s) 188, 201, 348
BsoBI CYCGRG 1 cut(s) 2303
Bsp119I TTCGAA 1 cut(s) 661
Bsp1286I GDGCHC 3 cut(s) 8, 371, 465
Bsp13I TCCGGA 1 cut(s) 100
Bsp143I GATC 4 cut(s) 220, 549, 2142, 2530
BspACI CCGC 5 cut(s) 66, 198, 202, 481, 1583
BspANI GGCC 3 cut(s) 188, 201, 348
BspCNI CTCAG 7 cut(s) 804, 881, 1032, 1045, 1243, 1480, 2308
BspEI TCCGGA 1 cut(s) 100
BspFNI CGCG 1 cut(s) 483
BspHI TCATGA 2 cut(s) 730, 2547
BspLI GGNNCC 2 cut(s) 349, 393
BspPI GGATC 1 cut(s) 228
BspT104I TTCGAA 1 cut(s) 661
BsrDI GCAATG 4 cut(s) 59, 1533, 2109, 2136
BsrI ACTGG 1 cut(s) 2024
BssECI CCNNGG 2 cut(s) 212, 2303
BssMI GATC 4 cut(s) 220, 549, 2142, 2530
BssSI CACGAG 1 cut(s) 41
Bst2BI CACGAG 1 cut(s) 41
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 3 cut(s) 1456, 1612, 1979
Bst6I CTCTTC 3 cut(s) 982, 1383, 2106
BstBI TTCGAA 1 cut(s) 661
BstC8I GCNNGC 3 cut(s) 25, 1130, 2481
BstENI CCTNNNNNAGG 1 cut(s) 2346
BstF5I GGATG 7 cut(s) 349, 852, 928, 1603, 1881, 2433, 2632
BstFNI CGCG 1 cut(s) 483
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 3 cut(s) 300, 2399, 2485
BstKTI GATC 4 cut(s) 223, 552, 2145, 2533
BstMAI GTCTC 7 cut(s) 40, 788, 893, 1163, 1228, 2108, 2436
BstMBI GATC 4 cut(s) 220, 549, 2142, 2530
BstMCI CGRYCG 1 cut(s) 202
BstMWI GCNNNNNNNGC 3 cut(s) 33, 314, 914
BstNI CCWGG 1 cut(s) 213
BstNSI RCATGY 1 cut(s) 180
BstSCI CCNGG 3 cut(s) 211, 2302, 2303
BstUI CGCG 1 cut(s) 483
BstV1I GCAGC 3 cut(s) 39, 2048, 2113
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BstZI CGGCCG 1 cut(s) 199
BsuRI GGCC 3 cut(s) 188, 201, 348
BtsCI GGATG 7 cut(s) 349, 852, 928, 1603, 1881, 2433, 2632
BtsI GCAGTG 2 cut(s) 655, 2412
BtsIMutI CAGTG 5 cut(s) 655, 682, 1617, 2412, 2540
Cac8I GCNNGC 3 cut(s) 25, 1130, 2481
CciI TCATGA 2 cut(s) 730, 2547
CciNI GCGGCCGC 1 cut(s) 199
CfoI GCGC 3 cut(s) 300, 2399, 2485
Cfr13I GGNCC 1 cut(s) 347
Cfr9I CCCGGG 1 cut(s) 2303
Csp6I GTAC 3 cut(s) 146, 767, 1789
CspCI CAANNNNNGTGG 2 cut(s) 1541, 1576
CviQI GTAC 3 cut(s) 146, 767, 1789
DpnI GATC 4 cut(s) 222, 551, 2144, 2532
DpnII GATC 4 cut(s) 220, 549, 2142, 2530
DraI TTTAAA 1 cut(s) 1806
DrdI GACNNNNNNGTC 1 cut(s) 2201
DseDI GACNNNNNNGTC 1 cut(s) 2201
EaeI YGGCCR 2 cut(s) 186, 199
EagI CGGCCG 1 cut(s) 199
Eam1104I CTCTTC 3 cut(s) 982, 1383, 2106
EarI CTCTTC 3 cut(s) 982, 1383, 2106
Ecl136II GAGCTC 2 cut(s) 369, 463
EclXI CGGCCG 1 cut(s) 199
Eco24I GRGCYC 2 cut(s) 371, 465
Eco32I GATATC 1 cut(s) 154
Eco52I CGGCCG 1 cut(s) 199
Eco53kI GAGCTC 2 cut(s) 369, 463
Eco57I CTGAAG 1 cut(s) 658
Eco88I CYCGRG 1 cut(s) 2303
EcoICRI GAGCTC 2 cut(s) 369, 463
EcoNI CCTNNNNNAGG 1 cut(s) 2346
EcoRII CCWGG 1 cut(s) 211
EcoRV GATATC 1 cut(s) 154
EcoT22I ATGCAT 1 cut(s) 1757
EcoT38I GRGCYC 2 cut(s) 371, 465
Esp3I CGTCTC 1 cut(s) 2436
FalI AAGNNNNNCTT 6 cut(s) 1645, 1677, 2448, 2480, 2463, 2495
FaqI GGGAC 2 cut(s) 154, 505
FauI CCCGC 1 cut(s) 73
FbaI TGATCA 2 cut(s) 549, 2530
FblI GTMKAC 2 cut(s) 939, 1587
Fnu4HI GCNGC 5 cut(s) 28, 199, 202, 2062, 2127
FokI GGATG 7 cut(s) 356, 839, 935, 1590, 1868, 2420, 2639
FriOI GRGCYC 2 cut(s) 371, 465
Fsp4HI GCNGC 5 cut(s) 28, 199, 202, 2062, 2127
FspBI CTAG 8 cut(s) 608, 1028, 1242, 1308, 2177, 2271, 2348, 2571
FspI TGCGCA 1 cut(s) 2484
GlaI GCGC 3 cut(s) 299, 2398, 2484
GluI GCNGC 5 cut(s) 28, 199, 202, 2062, 2127
GsuI CTGGAG 2 cut(s) 718, 950
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 3 cut(s) 188, 201, 348
HapII CCGG 2 cut(s) 101, 2304
HhaI GCGC 3 cut(s) 300, 2399, 2485
Hin6I GCGC 3 cut(s) 298, 2397, 2483
HinP1I GCGC 3 cut(s) 298, 2397, 2483
HincII GTYRAC 1 cut(s) 1185
HindII GTYRAC 1 cut(s) 1185
HpaII CCGG 2 cut(s) 101, 2304
HphI GGTGA 5 cut(s) 665, 1094, 1391, 1577, 1959
Hpy166II GTNNAC 7 cut(s) 361, 685, 940, 1185, 1588, 1990, 2182
Hpy8I GTNNAC 7 cut(s) 361, 685, 940, 1185, 1588, 1990, 2182
HpyAV CCTTC 6 cut(s) 188, 1364, 1671, 2013, 2116, 2387
HpyCH4III ACNGT 3 cut(s) 1456, 1612, 1979
HpyCH4IV ACGT 2 cut(s) 375, 2445
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 314, 914
HpySE526I ACGT 2 cut(s) 375, 2445
HspAI GCGC 3 cut(s) 298, 2397, 2483
Kpn2I TCCGGA 1 cut(s) 100
Ksp22I TGATCA 2 cut(s) 549, 2530
Kzo9I GATC 4 cut(s) 220, 549, 2142, 2530
LmnI GCTCC 4 cut(s) 295, 741, 808, 2037
Lsp1109I GCAGC 3 cut(s) 39, 2048, 2113
LweI GCATC 7 cut(s) 823, 1612, 1742, 2089, 2339, 2617, 2628
MaeI CTAG 8 cut(s) 608, 1028, 1242, 1308, 2177, 2271, 2348, 2571
MaeII ACGT 2 cut(s) 375, 2445
MaeIII GTNAC 3 cut(s) 1530, 2192, 2524
MalI GATC 4 cut(s) 222, 551, 2144, 2532
MboI GATC 4 cut(s) 220, 549, 2142, 2530
MfeI CAATTG 2 cut(s) 1617, 2423
MflI RGATCY 1 cut(s) 220
MhlI GDGCHC 3 cut(s) 8, 371, 465
MlsI TGGCCA 1 cut(s) 188
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 3 cut(s) 790, 941, 1788
MmeI TCCRAC 2 cut(s) 198, 1631
Mox20I TGGCCA 1 cut(s) 188
Mph1103I ATGCAT 1 cut(s) 1757
MroI TCCGGA 1 cut(s) 100
MroXI GAANNNNTTC 2 cut(s) 879, 1660
MscI TGGCCA 1 cut(s) 188
MseI TTAA 8 cut(s) 270, 503, 578, 1062, 1575, 1805, 2033, 2042
MslI CAYNNNNRTG 1 cut(s) 2210
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 1583
MspI CCGG 2 cut(s) 101, 2304
MspR9I CCNGG 3 cut(s) 213, 2304, 2305
MunI CAATTG 2 cut(s) 1617, 2423
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 483
MwoI GCNNNNNNNGC 3 cut(s) 33, 314, 914
NciI CCSGG 2 cut(s) 2304, 2305
NdeII GATC 4 cut(s) 220, 549, 2142, 2530
NlaIV GGNNCC 2 cut(s) 349, 393
NmuCI GTSAC 1 cut(s) 2192
NotI GCGGCCGC 1 cut(s) 199
NsbI TGCGCA 1 cut(s) 2484
NsiI ATGCAT 1 cut(s) 1757
NspI RCATGY 1 cut(s) 180
NspV TTCGAA 1 cut(s) 661
PagI TCATGA 2 cut(s) 730, 2547
PciI ACATGT 1 cut(s) 176
PdmI GAANNNNTTC 2 cut(s) 879, 1660
PkrI GCNGC 5 cut(s) 29, 200, 203, 2063, 2128
PleI GAGTC 3 cut(s) 790, 941, 1788
PpsI GAGTC 3 cut(s) 790, 941, 1788
PscI ACATGT 1 cut(s) 176
PshBI ATTAAT 1 cut(s) 1062
PsiI TTATAA 1 cut(s) 2226
Psp124BI GAGCTC 2 cut(s) 371, 465
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 2 cut(s) 349, 393
PspPI GGNCC 1 cut(s) 347
PsuI RGATCY 1 cut(s) 220
RsaI GTAC 3 cut(s) 147, 768, 1790
RsaNI GTAC 3 cut(s) 146, 767, 1789
RseI CAYNNNNRTG 1 cut(s) 2210
SacI GAGCTC 2 cut(s) 371, 465
SaqAI TTAA 8 cut(s) 270, 503, 578, 1062, 1575, 1805, 2033, 2042
SatI GCNGC 5 cut(s) 28, 199, 202, 2062, 2127
Sau3AI GATC 4 cut(s) 220, 549, 2142, 2530
Sau96I GGNCC 1 cut(s) 347
SchI GAGTC 3 cut(s) 790, 941, 1788
ScrFI CCNGG 3 cut(s) 213, 2304, 2305
SduI GDGCHC 3 cut(s) 8, 371, 465
SfaNI GCATC 7 cut(s) 823, 1612, 1742, 2089, 2339, 2617, 2628
SfuI TTCGAA 1 cut(s) 661
SmaI CCCGGG 1 cut(s) 2305
SmiMI CAYNNNNRTG 1 cut(s) 2210
SmlI CTYRAG 2 cut(s) 1302, 2379
SmoI CTYRAG 2 cut(s) 1302, 2379
SpeI ACTAGT 1 cut(s) 2176
SsiI CCGC 5 cut(s) 66, 198, 202, 481, 1583
SspI AATATT 1 cut(s) 1556
SspMI CTAG 8 cut(s) 608, 1028, 1242, 1308, 2177, 2271, 2348, 2571
SstI GAGCTC 2 cut(s) 371, 465
StyD4I CCNGG 3 cut(s) 211, 2302, 2303
TaaI ACNGT 3 cut(s) 1456, 1612, 1979
TaiI ACGT 2 cut(s) 378, 2448
TaqI TCGA 4 cut(s) 411, 661, 1512, 2333
TatI WGTACW 1 cut(s) 145
TauI GCSGC 2 cut(s) 201, 204
Tru1I TTAA 8 cut(s) 270, 503, 578, 1062, 1575, 1805, 2033, 2042
Tru9I TTAA 8 cut(s) 270, 503, 578, 1062, 1575, 1805, 2033, 2042
TscAI CASTG 5 cut(s) 655, 682, 1617, 2419, 2540
TseFI GTSAC 1 cut(s) 2192
TseI GCWGC 3 cut(s) 27, 2061, 2126
Tsp45I GTSAC 1 cut(s) 2192
TspGWI ACGGA 2 cut(s) 1654, 2191
TspMI CCCGGG 1 cut(s) 2303
TspRI CASTG 5 cut(s) 655, 682, 1617, 2419, 2540
VspI ATTAAT 1 cut(s) 1062
XagI CCTNNNNNAGG 1 cut(s) 2346
XapI RAATTY 9 cut(s) 60, 437, 451, 591, 617, 626, 876, 1402, 1953
XbaI TCTAGA 1 cut(s) 1241
XceI RCATGY 1 cut(s) 180
XcmI CCANNNNNNNNNTGG 1 cut(s) 1513
XmaI CCCGGG 1 cut(s) 2303
XmiI GTMKAC 2 cut(s) 939, 1587
XmnI GAANNNNTTC 2 cut(s) 879, 1660
XspI CTAG 8 cut(s) 608, 1028, 1242, 1308, 2177, 2271, 2348, 2571
Zsp2I ATGCAT 1 cut(s) 1757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.