Rw0G010530

gag-polypeptide of LTR copia-type

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00452
OG0000106
Physical Location & Seq
Reverse (-)
29249 .. 30890
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010530.1

Sequence Viewer

Length: 1155 bp
ATGACAAATACAGACGTACCAGAAAAACAGAAACCTTCAGGCAGCAGTATCATGGATGCATCAAATCCATATTACATTCATCCATCAGATCACCCCGGCCACGTACTTGTTCCATCAAAATTGAACGGGGCCAATTATTCAGACTGGAGTAAATCTATACTTCATGCCCTCACAGCAAAGAATAAAGTTGGCTTCATCAACGGTTCTGTTAAGCCTCCTTCACAAACAGAACAACCAACTGAATACGCTCTCTGGAATCAATGCAATAGTATGATTTTATCATGGCTTGCTAACTCAGTATGGGATGACTTCGAACATCAATTTTCACAACATAATGCACCAACAGTCTATCAGATTCAAAAAGCAATTGGCTCCCACTCACAAGGCACCATGACAGTCCAGACTTATTATACACAACTCAAAGGTCTCTGGGATGAATTAGAGACATATCGAGATCTTCCAGCTTGCAGTCAGATGGAAGCACATAATAAACAAAGAGAAGAAGATAGGCTGATGCAATTTCTTATGGGGCTTAGTGACACCTATAATGGTGTTCGCAGCAACATCCTCATGATGACACCATTGCCTAATGTTCGTCAAGCTTATTCAATCGTCAGTCAAGATGAGAAACAAAGACAGGTGACATCAGGATCAACTGAGAACTTCTCTATAGCTGCTGCAGTTCAGAATCGGGCAAGTAATTTTTCACCAAATTTCCTGAATAATTTCACAAACAAGCATTGTAGTCATTGCGACAAAGATGGCCATGCCATTGAAACATGTCGAATTCTGAAATACTATTGCACATTTTGTGACAAGAGAGGTCATACCGATGATAGATGCAGAAATAAAAGAAATCGTGGAAATCAAGGCAGCAGACAAAACAATAAATCACAACAGCAACGTGGAGGCAACCCACATGGTTCCTTCCATGCCGCCAATGCAACAGATACAAGCCTTATCCTTCCTCCACAAGAATCCCAGGCGTCAACTTCAGCATCTTTGCCAAATCCTTGGCATAGCCTTTCGCCAGAGCAACTTCAGCAATTGGCTCAGACCATTTCTATGATGACCACACAAAATGCTTATGGTAACAACAACGCTTTTGCAAATGCTGCAGGACTTGGCTACGGGGAAGATGATTGGCTCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_3 Retrotrans_gag

Protein Analysis

384

Amino Acids

43.06

Weight (kDa)

6.94

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 27 - 73 9.1e-19 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000106)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20980 AT3G21000
fragaria_vesca FvH4_1g15931 FvH4_1g15931 FvH4_1g18381 FvH4_1g18382 FvH4_1g22571 FvH4_1g24950 FvH4_1g29372 FvH4_2g03582 FvH4_2g09851 FvH4_2g13112 FvH4_2g20843 FvH4_2g22701 FvH4_2g22702 FvH4_2g23403 FvH4_2g40201 FvH4_3g03542 FvH4_3g03543 FvH4_3g04761 FvH4_3g10464 FvH4_3g12741 FvH4_3g15781 FvH4_3g30981 FvH4_4g00985 FvH4_4g07035 FvH4_4g08192 FvH4_4g08211 FvH4_4g08553 FvH4_4g14242 FvH4_4g14243 FvH4_4g14641 FvH4_4g14843 FvH4_4g14844 FvH4_4g15061 FvH4_4g16281 FvH4_4g19152 FvH4_4g26451 FvH4_5g06442 FvH4_5g22521 FvH4_5g24301 FvH4_5g38641 FvH4_6g02393 FvH4_6g12782 FvH4_6g13871 FvH4_6g19391 FvH4_6g20105 FvH4_6g21292 FvH4_6g23581 FvH4_6g29153 FvH4_6g29691 FvH4_6g30871 FvH4_6g33351 FvH4_6g37042 FvH4_6g38781 FvH4_6g40340 FvH4_7g11221 FvH4_7g23352 FvH4_7g23352 FvH4_7g31212 FvH4_7g32172 FvH4_7g32173 FvH4_7g33701
malus_domestica MD03G1006900.v1.1 MD04G1039100.v1.1 MD04G1239100.v1.1 MD05G1048700.v1.1 MD08G1001800.v1.1 MD09G1165800.v1.1 MD11G1065200.v1.1 MD11G1138200.v1.1 MD14G1033300.v1.1 MD14G1173900.v1.1 MD15G1218200.v1.1 MD16G1034000.v1.1
prunus_persica Prupe.1G219500_v2.0.a1 Prupe.2G169400_v2.0.a1 Prupe.4G109700_v2.0.a1 Prupe.7G022000_v2.0.a1
pyrus_communis pycom01g02000 pycom02g13350 pycom03g01010 pycom04g05000 pycom04g06100 pycom05g05580 pycom05g09950 pycom05g13280 pycom05g26610 pycom06g05940 pycom06g07760 pycom07g06120 pycom07g24780 pycom08g08820 pycom09g03600 pycom09g07200 pycom10g01280 pycom10g06730 pycom10g08410 pycom10g25520 pycom11g00470 pycom11g05250 pycom11g25180 pycom12g08050 pycom13g14760 pycom13g28170 pycom15g14310 pycom15g19880 pycom15g24230 pycom15g33640 pycom16g02150 pycom16g16240 pycom16g20050 pycom16g21090 pycom17g06100
rosa_chinensis RchiOBHm_Chr1g0322161 RchiOBHm_Chr1g0326341 RchiOBHm_Chr1g0357701 RchiOBHm_Chr1g0379861 RchiOBHm_Chr1g0383421 RchiOBHm_Chr2g0093981 RchiOBHm_Chr2g0139051 RchiOBHm_Chr2g0145501 RchiOBHm_Chr3g0450971 RchiOBHm_Chr3g0451441 RchiOBHm_Chr3g0457411 RchiOBHm_Chr4g0436261 RchiOBHm_Chr5g0051431 RchiOBHm_Chr5g0064031 RchiOBHm_Chr5g0073141 RchiOBHm_Chr5g0080061 RchiOBHm_Chr7g0200561 RchiOBHm_Chr7g0235911
rosa_laevigata RLG00000000145 RLG00000000429 RLG00000020339 RLG00000035212
rosa_multiflora Rmu_co7977988.1_g000001 Rmu_co8037866.1_g000001 Rmu_co8243895.1_g000001 Rmu_co8363787.1_g000001 Rmu_co8381003.1_g000001 Rmu_sc0000263.1_g000006 Rmu_sc0000349.1_g000002 Rmu_sc0000455.1_g000031 Rmu_sc0000936.1_g000022 Rmu_sc0001035.1_g000023 Rmu_sc0001169.1_g000019 Rmu_sc0001250.1_g000026 Rmu_sc0002200.1_g000051 Rmu_sc0002230.1_g000027 Rmu_sc0002591.1_g000039 Rmu_sc0003182.1_g000003 Rmu_sc0003441.1_g000068 Rmu_sc0003722.1_g000018 Rmu_sc0003764.1_g000007 Rmu_sc0006014.1_g000016 Rmu_sc0012582.1_g000002 Rmu_sc0013519.1_g000002 Rmu_sc0014550.1_g000005 Rmu_sc0014857.1_g000007 Rmu_sc0019855.1_g000001 Rmu_sc0030839.1_g000001 Rmu_sc0031308.1_g000001
rosa_roxburghii Rroxscaffold_1G00011290 Rroxscaffold_1G00041340 Rroxscaffold_1G00053780 Rroxscaffold_1G00059130 Rroxscaffold_1G00061580 Rroxscaffold_2G00080940 Rroxscaffold_2G00109540 Rroxscaffold_3G00228400 Rroxscaffold_3G00237710 Rroxscaffold_4G00285210 Rroxscaffold_4G00299600 Rroxscaffold_4G00324940 Rroxscaffold_4G00330580 Rroxscaffold_5G00333000 Rroxscaffold_5G00347920 Rroxscaffold_5G00375310 Rroxscaffold_6G00394760 Rroxscaffold_6G00406260 Rroxscaffold_7G00165690 Rroxscaffold_7G00187680
rosa_rugosa Rorug01G0050200 Rorug02G0273800 Rorug06G0490600 Rorug06G0490600 Rorug06G0490700
rosa_samantha Rh1AG348400 Rh5AG222600 Rh6DG000500 Rh6DG194500 Rh7BG452700
rosa_wichuraiana Rw0G003060 Rw0G010530 Rw0G012970 Rw0G022560 Rw0G023390 Rw1G001270 Rw1G001530 Rw1G006070 Rw1G008210 Rw1G022090 Rw1G023320 Rw2G003790 Rw2G004140 Rw2G005280 Rw2G006460 Rw2G019620 Rw2G019840 Rw2G022530 Rw2G023280 Rw2G024990 Rw2G042380 Rw2G051390 Rw3G001640 Rw3G016720 Rw3G017100 Rw3G020890 Rw3G020900 Rw3G021100 Rw4G004820 Rw4G014950 Rw4G030920 Rw4G031400 Rw5G008500 Rw5G017950 Rw5G020170 Rw5G030160 Rw5G033050 Rw5G034640 Rw5G043920 Rw6G005060 Rw6G005540 Rw6G006720 Rw6G008460 Rw6G008670 Rw6G014780 Rw6G019100 Rw6G019350 Rw6G026310 Rw7G016810 Rw7G019180 Rw7G022890 Rw7G025880 Rw7G026390 Rw7G028340 Rw7G034850 Rw7G035170 Rw7G035420 Rw7G037990 Rw7G041560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 386
AciI CCGC 1 cut(s) 936
AclWI GGATC 1 cut(s) 658
AcoI YGGCCR 2 cut(s) 97, 763
AcsI RAATTY 2 cut(s) 712, 786
AcuI CTGAAG 3 cut(s) 21, 978, 1025
AcyI GRCGYC 1 cut(s) 986
AfaI GTAC 2 cut(s) 18, 105
AflIII ACRYGT 1 cut(s) 779
AgsI TTSAA 4 cut(s) 124, 359, 609, 776
AjnI CCWGG 1 cut(s) 981
AluBI AGCT 3 cut(s) 464, 602, 674
AluI AGCT 3 cut(s) 464, 602, 674
Alw26I GTCTC 2 cut(s) 431, 437
AlwI GGATC 1 cut(s) 658
AoxI GGCC 3 cut(s) 97, 129, 763
ApeKI GCWGC 6 cut(s) 42, 558, 674, 677, 873, 1115
ApoI RAATTY 2 cut(s) 712, 786
Asp700I GAANNNNTTC 1 cut(s) 725
AspS9I GGNCC 1 cut(s) 129
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 3 cut(s) 83, 652, 699
AsuII TTCGAA 1 cut(s) 312
BaeI ACNNNNGTAYC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 765
BanI GGYRCC 1 cut(s) 386
BbvI GCAGC 6 cut(s) 54, 570, 661, 664, 885, 1102
BccI CCATC 4 cut(s) 91, 121, 469, 755
BciT130I CCWGG 1 cut(s) 983
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 2 cut(s) 431, 437
BfmI CTRYAG 3 cut(s) 669, 678, 1116
BglII AGATCT 1 cut(s) 454
BisI GCNGC 7 cut(s) 43, 559, 675, 678, 874, 936, 1116
BlsI GCNGC 7 cut(s) 44, 560, 676, 679, 875, 937, 1117
Bme1390I CCNGG 2 cut(s) 96, 983
BmgT120I GGNCC 1 cut(s) 129
BmiI GGNNCC 4 cut(s) 130, 373, 388, 925
BmrFI CCNGG 2 cut(s) 96, 983
BmsI GCATC 5 cut(s) 46, 68, 504, 830, 1007
BplI GAGNNNNNCTC 2 cut(s) 650, 682
BpmI CTGGAG 1 cut(s) 166
Bpu10I CCTNAGC 1 cut(s) 1148
Bpu14I TTCGAA 1 cut(s) 312
BpuMI CCSGG 1 cut(s) 96
BsaAI YACGTR 1 cut(s) 103
BsaHI GRCGYC 1 cut(s) 986
BsaI GGTCTC 1 cut(s) 431
BsaJI CCNNGG 3 cut(s) 94, 981, 1013
Bse1I ACTGG 1 cut(s) 149
Bse3DI GCAATG 2 cut(s) 581, 748
BseBI CCWGG 1 cut(s) 983
BseDI CCNNGG 3 cut(s) 94, 981, 1013
BseGI GGATG 5 cut(s) 61, 79, 310, 439, 564
BseMI GCAATG 2 cut(s) 581, 748
BseMII CTCAG 3 cut(s) 309, 648, 1067
BseNI ACTGG 1 cut(s) 149
BseXI GCAGC 6 cut(s) 54, 570, 661, 664, 885, 1102
BshFI GGCC 3 cut(s) 99, 131, 765
BshNI GGYRCC 1 cut(s) 386
BsiSI CCGG 1 cut(s) 96
BsmAI GTCTC 2 cut(s) 431, 437
BsnI GGCC 3 cut(s) 99, 131, 765
Bso31I GGTCTC 1 cut(s) 431
Bsp119I TTCGAA 1 cut(s) 312
Bsp143I GATC 3 cut(s) 88, 454, 650
BspACI CCGC 1 cut(s) 936
BspANI GGCC 3 cut(s) 99, 131, 765
BspCNI CTCAG 3 cut(s) 308, 649, 1066
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 4 cut(s) 130, 373, 388, 925
BspMAI CTGCAG 2 cut(s) 682, 1120
BspPI GGATC 1 cut(s) 658
BspT104I TTCGAA 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 431
BsrDI GCAATG 2 cut(s) 581, 748
BsrI ACTGG 1 cut(s) 149
BssECI CCNNGG 3 cut(s) 94, 981, 1013
BssMI GATC 3 cut(s) 88, 454, 650
BssNI GRCGYC 1 cut(s) 986
BssT1I CCWWGG 1 cut(s) 1013
Bst2UI CCWGG 1 cut(s) 983
Bst4CI ACNGT 3 cut(s) 203, 346, 397
BstACI GRCGYC 1 cut(s) 986
BstAPI GCANNNNNTGC 1 cut(s) 1115
BstBAI YACGTR 1 cut(s) 103
BstBI TTCGAA 1 cut(s) 312
BstC8I GCNNGC 2 cut(s) 288, 466
BstDEI CTNAG 5 cut(s) 295, 533, 657, 1053, 1148
BstF5I GGATG 5 cut(s) 61, 79, 310, 439, 564
BstKTI GATC 3 cut(s) 91, 457, 653
BstMAI GTCTC 2 cut(s) 431, 437
BstMBI GATC 3 cut(s) 88, 454, 650
BstMWI GCNNNNNNNGC 4 cut(s) 173, 941, 1042, 1115
BstNI CCWGG 1 cut(s) 983
BstNSI RCATGY 1 cut(s) 783
BstSCI CCNGG 2 cut(s) 94, 981
BstSFI CTRYAG 3 cut(s) 669, 678, 1116
BstV1I GCAGC 6 cut(s) 54, 570, 661, 664, 885, 1102
BstX2I RGATCY 1 cut(s) 454
BstXI CCANNNNNNTGG 1 cut(s) 1014
BstYI RGATCY 1 cut(s) 454
BsuRI GGCC 3 cut(s) 99, 131, 765
BtsCI GGATG 5 cut(s) 61, 79, 310, 439, 564
Cac8I GCNNGC 2 cut(s) 288, 466
CciI TCATGA 1 cut(s) 570
Cfr13I GGNCC 1 cut(s) 129
CseI GACGC 1 cut(s) 975
Csp6I GTAC 2 cut(s) 17, 104
CviAII CATG 9 cut(s) 52, 164, 282, 391, 571, 767, 780, 920, 932
CviQI GTAC 2 cut(s) 17, 104
DdeI CTNAG 5 cut(s) 295, 533, 657, 1053, 1148
DpnI GATC 3 cut(s) 90, 456, 652
DpnII GATC 3 cut(s) 88, 454, 650
EaeI YGGCCR 2 cut(s) 97, 763
Eco130I CCWWGG 1 cut(s) 1013
Eco31I GGTCTC 1 cut(s) 431
Eco57I CTGAAG 3 cut(s) 21, 978, 1025
EcoRI GAATTC 1 cut(s) 786
EcoRII CCWGG 1 cut(s) 981
EcoT14I CCWWGG 1 cut(s) 1013
EcoT22I ATGCAT 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 1013
FaeI CATG 9 cut(s) 55, 167, 285, 394, 574, 770, 783, 923, 935
FatI CATG 9 cut(s) 51, 163, 281, 390, 570, 766, 779, 919, 931
Fnu4HI GCNGC 7 cut(s) 43, 559, 675, 678, 874, 936, 1116
FokI GGATG 5 cut(s) 66, 68, 317, 446, 551
Fsp4HI GCNGC 7 cut(s) 43, 559, 675, 678, 874, 936, 1116
GluI GCNGC 7 cut(s) 43, 559, 675, 678, 874, 936, 1116
GsuI CTGGAG 1 cut(s) 166
HaeIII GGCC 3 cut(s) 99, 131, 765
HapII CCGG 1 cut(s) 96
HgaI GACGC 1 cut(s) 975
Hin1I GRCGYC 1 cut(s) 986
Hin1II CATG 9 cut(s) 55, 167, 285, 394, 574, 770, 783, 923, 935
HincII GTYRAC 1 cut(s) 990
HindII GTYRAC 1 cut(s) 990
HindIII AAGCTT 1 cut(s) 600
HinfI GANTC 4 cut(s) 256, 355, 688, 977
HpaII CCGG 1 cut(s) 96
HphI GGTGA 3 cut(s) 83, 652, 699
Hpy166II GTNNAC 1 cut(s) 990
Hpy188I TCNGA 7 cut(s) 88, 142, 354, 474, 687, 792, 1056
Hpy188III TCNNGA 7 cut(s) 253, 400, 452, 571, 620, 648, 718
Hpy8I GTNNAC 1 cut(s) 990
HpyAV CCTTC 4 cut(s) 45, 228, 937, 974
HpyCH4III ACNGT 3 cut(s) 203, 346, 397
HpyCH4IV ACGT 3 cut(s) 15, 102, 904
HpyF10VI GCNNNNNNNGC 4 cut(s) 173, 941, 1042, 1115
HpyF3I CTNAG 5 cut(s) 295, 533, 657, 1053, 1148
HpySE526I ACGT 3 cut(s) 15, 102, 904
Hsp92I GRCGYC 1 cut(s) 986
Hsp92II CATG 9 cut(s) 55, 167, 285, 394, 574, 770, 783, 923, 935
Kzo9I GATC 3 cut(s) 88, 454, 650
LmnI GCTCC 1 cut(s) 377
Lsp1109I GCAGC 6 cut(s) 54, 570, 661, 664, 885, 1102
LweI GCATC 5 cut(s) 46, 68, 504, 830, 1007
MaeII ACGT 3 cut(s) 15, 102, 904
MaeIII GTNAC 4 cut(s) 536, 640, 812, 1091
MalI GATC 3 cut(s) 90, 456, 652
MboI GATC 3 cut(s) 88, 454, 650
MboII GAAGA 4 cut(s) 449, 512, 515, 1148
MfeI CAATTG 2 cut(s) 366, 1046
MflI RGATCY 1 cut(s) 454
MlsI TGGCCA 1 cut(s) 765
MluNI TGGCCA 1 cut(s) 765
MnlI CCTC 6 cut(s) 179, 225, 578, 815, 902, 978
Mox20I TGGCCA 1 cut(s) 765
Mph1103I ATGCAT 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 725
MscI TGGCCA 1 cut(s) 765
MseI TTAA 1 cut(s) 210
MslI CAYNNNNRTG 3 cut(s) 569, 831, 1064
Msp20I TGGCCA 1 cut(s) 765
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 2 cut(s) 96, 983
MunI CAATTG 2 cut(s) 366, 1046
MvaI CCWGG 1 cut(s) 983
MwoI GCNNNNNNNGC 4 cut(s) 173, 941, 1042, 1115
NciI CCSGG 1 cut(s) 96
NdeII GATC 3 cut(s) 88, 454, 650
NlaIII CATG 9 cut(s) 55, 167, 285, 394, 574, 770, 783, 923, 935
NlaIV GGNNCC 4 cut(s) 130, 373, 388, 925
NmuCI GTSAC 3 cut(s) 536, 640, 812
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 1 cut(s) 783
NspV TTCGAA 1 cut(s) 312
PagI TCATGA 1 cut(s) 570
PciI ACATGT 1 cut(s) 779
PdmI GAANNNNTTC 1 cut(s) 725
PfeI GAWTC 4 cut(s) 256, 355, 688, 977
PkrI GCNGC 7 cut(s) 44, 560, 676, 679, 875, 937, 1117
Ppu21I YACGTR 1 cut(s) 103
PscI ACATGT 1 cut(s) 779
Psp6I CCWGG 1 cut(s) 981
PspGI CCWGG 1 cut(s) 981
PspN4I GGNNCC 4 cut(s) 130, 373, 388, 925
PspPI GGNCC 1 cut(s) 129
PstI CTGCAG 2 cut(s) 682, 1120
PsuI RGATCY 1 cut(s) 454
RsaI GTAC 2 cut(s) 18, 105
RsaNI GTAC 2 cut(s) 17, 104
RseI CAYNNNNRTG 3 cut(s) 569, 831, 1064
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 7 cut(s) 43, 559, 675, 678, 874, 936, 1116
Sau3AI GATC 3 cut(s) 88, 454, 650
Sau96I GGNCC 1 cut(s) 129
ScrFI CCNGG 2 cut(s) 96, 983
SfaNI GCATC 5 cut(s) 46, 68, 504, 830, 1007
SfcI CTRYAG 3 cut(s) 669, 678, 1116
SfuI TTCGAA 1 cut(s) 312
SmiMI CAYNNNNRTG 3 cut(s) 569, 831, 1064
SsiI CCGC 1 cut(s) 936
StyD4I CCNGG 2 cut(s) 94, 981
StyI CCWWGG 1 cut(s) 1013
TaaI ACNGT 3 cut(s) 203, 346, 397
TaiI ACGT 3 cut(s) 18, 105, 907
TaqI TCGA 3 cut(s) 312, 451, 784
TauI GCSGC 1 cut(s) 938
TfiI GAWTC 4 cut(s) 256, 355, 688, 977
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TseFI GTSAC 3 cut(s) 536, 640, 812
TseI GCWGC 6 cut(s) 42, 558, 674, 677, 873, 1115
Tsp45I GTSAC 3 cut(s) 536, 640, 812
TspDTI ATGAA 4 cut(s) 68, 152, 184, 450
XapI RAATTY 2 cut(s) 712, 786
XceI RCATGY 1 cut(s) 783
XmnI GAANNNNTTC 1 cut(s) 725
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.