RchiOBHm_Chr3g0450971

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000106
Physical Location & Seq
Reverse (-)
2267706 .. 2268846
1141 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41828

Sequence Viewer

Length: 909 bp
ATGGTTATGGCATCGTCGTTTGGGTCATTTGTCGTTTGGATATCTTCGAAAGTTGAAGCCTCATTTGTTTTCAGTTGTGCTAAATACTTTGTTACTTTTATTGATGAGTGCACCCGGATGACATGGGTAATGTTGATGAAGAATAAGTGGGAATTATGTCAGGCGTTTCAAGCCTTTCACAAGATGGTTTCTAGCCAGTACCAGAGAAAGATTCAGGTCCTTCAGTCAGATAATGGGGGTGAGTATACGAGTAATGCCATGGCAGAGTTTTGCCAGTCTCAAGGCATTCGCCATCAAACTTCTTGTGCTTACACTCCGCAGCAAAATGGTCTAGCAGAACGAAAGAATTGTCAGTTGTTGGAAGTTCTTCGTGCCTCTCTCTTTGGAATGAATGTGCCTCGTGAATATTGGGGTGAAGCGTTGAAGTCTGCAACATATCTCATTAACCGCACTCCTTCTAGTGTCATTAATTTTGAGACTCCTCAACAAAAGTTAGGTAGATTGATCTCTGCTCCTACTTTACCTAATTTGGAACCTCGAGTCTTTGGGTGCGTAGCATATGTACACATTCCTAAACATCTACGGCACAAACTTGATCCATGTGCCAAGAGATGTCTCTTTGTTGGTTATGCGGAGTTTAAAAAGGGGTATCGGTGTTATGATCCTCAGACGAAGACTCTCCATGTCTCTTTGGATGTATCTTTTCATGAAAATGAGGCTTACTTTACAGGCAAAGCTCATGAGCCTTCTCTTCAGGGGGAGAAAAGGTATATTGAAGAGAATTCTCAGGATATTTTTTCTGAAATTGAAGAGGTGGAGGAAAGATTGGTACAGCAGATTCTTTTCAACCCTTCAAAGGAAGGCACTAACTTGTCACATTCCACTCCAGCTGCTCCTGCCAATCCATAG

Protein Analysis

302

Amino Acids

34.47

Weight (kDa)

7.99

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
rve PF00665 28 - 106 2.9e-15 Integrase core domain
SH3_retrovirus PF25597 184 - 242 9.2e-25 Retroviral polymerase SH3-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000106)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20980 AT3G21000
fragaria_vesca FvH4_1g15931 FvH4_1g15931 FvH4_1g18381 FvH4_1g18382 FvH4_1g22571 FvH4_1g24950 FvH4_1g29372 FvH4_2g03582 FvH4_2g09851 FvH4_2g13112 FvH4_2g20843 FvH4_2g22701 FvH4_2g22702 FvH4_2g23403 FvH4_2g40201 FvH4_3g03542 FvH4_3g03543 FvH4_3g04761 FvH4_3g10464 FvH4_3g12741 FvH4_3g15781 FvH4_3g30981 FvH4_4g00985 FvH4_4g07035 FvH4_4g08192 FvH4_4g08211 FvH4_4g08553 FvH4_4g14242 FvH4_4g14243 FvH4_4g14641 FvH4_4g14843 FvH4_4g14844 FvH4_4g15061 FvH4_4g16281 FvH4_4g19152 FvH4_4g26451 FvH4_5g06442 FvH4_5g22521 FvH4_5g24301 FvH4_5g38641 FvH4_6g02393 FvH4_6g12782 FvH4_6g13871 FvH4_6g19391 FvH4_6g20105 FvH4_6g21292 FvH4_6g23581 FvH4_6g29153 FvH4_6g29691 FvH4_6g30871 FvH4_6g33351 FvH4_6g37042 FvH4_6g38781 FvH4_6g40340 FvH4_7g11221 FvH4_7g23352 FvH4_7g23352 FvH4_7g31212 FvH4_7g32172 FvH4_7g32173 FvH4_7g33701
malus_domestica MD03G1006900.v1.1 MD04G1039100.v1.1 MD04G1239100.v1.1 MD05G1048700.v1.1 MD08G1001800.v1.1 MD09G1165800.v1.1 MD11G1065200.v1.1 MD11G1138200.v1.1 MD14G1033300.v1.1 MD14G1173900.v1.1 MD15G1218200.v1.1 MD16G1034000.v1.1
prunus_persica Prupe.1G219500_v2.0.a1 Prupe.2G169400_v2.0.a1 Prupe.4G109700_v2.0.a1 Prupe.7G022000_v2.0.a1
pyrus_communis pycom01g02000 pycom02g13350 pycom03g01010 pycom04g05000 pycom04g06100 pycom05g05580 pycom05g09950 pycom05g13280 pycom05g26610 pycom06g05940 pycom06g07760 pycom07g06120 pycom07g24780 pycom08g08820 pycom09g03600 pycom09g07200 pycom10g01280 pycom10g06730 pycom10g08410 pycom10g25520 pycom11g00470 pycom11g05250 pycom11g25180 pycom12g08050 pycom13g14760 pycom13g28170 pycom15g14310 pycom15g19880 pycom15g24230 pycom15g33640 pycom16g02150 pycom16g16240 pycom16g20050 pycom16g21090 pycom17g06100
rosa_chinensis RchiOBHm_Chr1g0322161 RchiOBHm_Chr1g0326341 RchiOBHm_Chr1g0357701 RchiOBHm_Chr1g0379861 RchiOBHm_Chr1g0383421 RchiOBHm_Chr2g0093981 RchiOBHm_Chr2g0139051 RchiOBHm_Chr2g0145501 RchiOBHm_Chr3g0450971 RchiOBHm_Chr3g0451441 RchiOBHm_Chr3g0457411 RchiOBHm_Chr4g0436261 RchiOBHm_Chr5g0051431 RchiOBHm_Chr5g0064031 RchiOBHm_Chr5g0073141 RchiOBHm_Chr5g0080061 RchiOBHm_Chr7g0200561 RchiOBHm_Chr7g0235911
rosa_laevigata RLG00000000145 RLG00000000429 RLG00000020339 RLG00000035212
rosa_multiflora Rmu_co7977988.1_g000001 Rmu_co8037866.1_g000001 Rmu_co8243895.1_g000001 Rmu_co8363787.1_g000001 Rmu_co8381003.1_g000001 Rmu_sc0000263.1_g000006 Rmu_sc0000349.1_g000002 Rmu_sc0000455.1_g000031 Rmu_sc0000936.1_g000022 Rmu_sc0001035.1_g000023 Rmu_sc0001169.1_g000019 Rmu_sc0001250.1_g000026 Rmu_sc0002200.1_g000051 Rmu_sc0002230.1_g000027 Rmu_sc0002591.1_g000039 Rmu_sc0003182.1_g000003 Rmu_sc0003441.1_g000068 Rmu_sc0003722.1_g000018 Rmu_sc0003764.1_g000007 Rmu_sc0006014.1_g000016 Rmu_sc0012582.1_g000002 Rmu_sc0013519.1_g000002 Rmu_sc0014550.1_g000005 Rmu_sc0014857.1_g000007 Rmu_sc0019855.1_g000001 Rmu_sc0030839.1_g000001 Rmu_sc0031308.1_g000001
rosa_roxburghii Rroxscaffold_1G00011290 Rroxscaffold_1G00041340 Rroxscaffold_1G00053780 Rroxscaffold_1G00059130 Rroxscaffold_1G00061580 Rroxscaffold_2G00080940 Rroxscaffold_2G00109540 Rroxscaffold_3G00228400 Rroxscaffold_3G00237710 Rroxscaffold_4G00285210 Rroxscaffold_4G00299600 Rroxscaffold_4G00324940 Rroxscaffold_4G00330580 Rroxscaffold_5G00333000 Rroxscaffold_5G00347920 Rroxscaffold_5G00375310 Rroxscaffold_6G00394760 Rroxscaffold_6G00406260 Rroxscaffold_7G00165690 Rroxscaffold_7G00187680
rosa_rugosa Rorug01G0050200 Rorug02G0273800 Rorug06G0490600 Rorug06G0490600 Rorug06G0490700
rosa_samantha Rh1AG348400 Rh5AG222600 Rh6DG000500 Rh6DG194500 Rh7BG452700
rosa_wichuraiana Rw0G003060 Rw0G010530 Rw0G012970 Rw0G022560 Rw0G023390 Rw1G001270 Rw1G001530 Rw1G006070 Rw1G008210 Rw1G022090 Rw1G023320 Rw2G003790 Rw2G004140 Rw2G005280 Rw2G006460 Rw2G019620 Rw2G019840 Rw2G022530 Rw2G023280 Rw2G024990 Rw2G042380 Rw2G051390 Rw3G001640 Rw3G016720 Rw3G017100 Rw3G020890 Rw3G020900 Rw3G021100 Rw4G004820 Rw4G014950 Rw4G030920 Rw4G031400 Rw5G008500 Rw5G017950 Rw5G020170 Rw5G030160 Rw5G033050 Rw5G034640 Rw5G043920 Rw6G005060 Rw6G005540 Rw6G006720 Rw6G008460 Rw6G008670 Rw6G014780 Rw6G019100 Rw6G019350 Rw6G026310 Rw7G016810 Rw7G019180 Rw7G022890 Rw7G025880 Rw7G026390 Rw7G028340 Rw7G034850 Rw7G035170 Rw7G035420 Rw7G037990 Rw7G041560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 245
AciI CCGC 3 cut(s) 317, 448, 632
AclWI GGATC 2 cut(s) 590, 656
AcsI RAATTY 1 cut(s) 781
AcuI CTGAAG 2 cut(s) 206, 737
AfaI GTAC 3 cut(s) 200, 564, 831
AfiI CCNNNNNNNGG 1 cut(s) 856
AgsI TTSAA 7 cut(s) 56, 170, 424, 776, 809, 847, 855
AluBI AGCT 2 cut(s) 737, 890
AluI AGCT 2 cut(s) 737, 890
Alw21I GWGCWC 1 cut(s) 113
Alw26I GTCTC 4 cut(s) 282, 470, 620, 691
Alw44I GTGCAC 1 cut(s) 109
AlwI GGATC 2 cut(s) 590, 656
Ama87I CYCGRG 1 cut(s) 537
ApaLI GTGCAC 1 cut(s) 109
ApeKI GCWGC 2 cut(s) 319, 890
ApoI RAATTY 1 cut(s) 781
ArsI GACNNNNNNTTYG 2 cut(s) 334, 366
AseI ATTAAT 1 cut(s) 468
Asp700I GAANNNNTTC 1 cut(s) 366
AspS9I GGNCC 1 cut(s) 217
AsuC2I CCSGG 1 cut(s) 115
AsuHPI GGTGA 2 cut(s) 251, 425
AsuII TTCGAA 1 cut(s) 47
AvaI CYCGRG 1 cut(s) 537
AvaII GGWCC 1 cut(s) 217
BaeGI GKGCMC 1 cut(s) 113
BauI CACGAG 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 680
Bbv12I GWGCWC 1 cut(s) 113
BbvI GCAGC 2 cut(s) 331, 877
BccI CCATC 2 cut(s) 178, 300
BceAI ACGGC 1 cut(s) 599
BcnI CCSGG 1 cut(s) 115
BcoDI GTCTC 4 cut(s) 282, 470, 620, 691
BfaI CTAG 3 cut(s) 192, 332, 459
BisI GCNGC 2 cut(s) 320, 891
BlsI GCNGC 2 cut(s) 321, 892
Bme1390I CCNGG 1 cut(s) 115
Bme18I GGWCC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 537
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 1 cut(s) 534
BmrFI CCNGG 1 cut(s) 115
BmsI GCATC 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 680
BpmI CTGGAG 1 cut(s) 870
Bpu14I TTCGAA 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 264
BpuMI CCSGG 1 cut(s) 115
BsaJI CCNNGG 1 cut(s) 258
BsaXI ACNNNNNCTCC 2 cut(s) 752, 782
Bsc4I CCNNNNNNNGG 1 cut(s) 856
Bse1I ACTGG 2 cut(s) 196, 274
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 2 cut(s) 123, 700
BseLI CCNNNNNNNGG 1 cut(s) 856
BseMII CTCAG 2 cut(s) 680, 800
BseNI ACTGG 2 cut(s) 196, 274
BseRI GAGGAG 1 cut(s) 471
BseSI GKGCMC 1 cut(s) 113
BseXI GCAGC 2 cut(s) 331, 877
BsiHKAI GWGCWC 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 537
BsiSI CCGG 1 cut(s) 115
BslI CCNNNNNNNGG 1 cut(s) 856
BsmAI GTCTC 4 cut(s) 282, 470, 620, 691
BsmI GAATGC 1 cut(s) 285
BsoBI CYCGRG 1 cut(s) 537
Bsp119I TTCGAA 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 113
Bsp1407I TGTACA 1 cut(s) 562
Bsp143I GATC 3 cut(s) 504, 595, 661
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 3 cut(s) 317, 448, 632
BspCNI CTCAG 2 cut(s) 679, 799
BspHI TCATGA 2 cut(s) 706, 739
BspLI GGNNCC 1 cut(s) 534
BspPI GGATC 2 cut(s) 590, 656
BspT104I TTCGAA 1 cut(s) 47
BsrGI TGTACA 1 cut(s) 562
BsrI ACTGG 2 cut(s) 196, 274
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 3 cut(s) 504, 595, 661
BssNAI GTATAC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 258
Bst1107I GTATAC 1 cut(s) 246
Bst2BI CACGAG 1 cut(s) 399
Bst6I CTCTTC 3 cut(s) 756, 771, 804
BstAUI TGTACA 1 cut(s) 562
BstBI TTCGAA 1 cut(s) 47
BstDEI CTNAG 2 cut(s) 666, 786
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 2 cut(s) 123, 700
BstKTI GATC 3 cut(s) 507, 598, 664
BstMAI GTCTC 4 cut(s) 282, 470, 620, 691
BstMBI GATC 3 cut(s) 504, 595, 661
BstMWI GCNNNNNNNGC 2 cut(s) 170, 896
BstSCI CCNGG 1 cut(s) 113
BstSLI GKGCMC 1 cut(s) 113
BstV1I GCAGC 2 cut(s) 331, 877
BstV2I GAAGAC 1 cut(s) 680
BstZ17I GTATAC 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 2 cut(s) 123, 700
CciI TCATGA 2 cut(s) 706, 739
Cfr13I GGNCC 1 cut(s) 217
Csp6I GTAC 3 cut(s) 199, 563, 830
CviAII CATG 6 cut(s) 123, 259, 600, 683, 707, 740
CviJI RGCY 7 cut(s) 59, 173, 195, 719, 737, 745, 890
CviKI_1 RGCY 7 cut(s) 59, 173, 195, 719, 737, 745, 890
CviQI GTAC 3 cut(s) 199, 563, 830
DdeI CTNAG 2 cut(s) 666, 786
DpnI GATC 3 cut(s) 506, 597, 663
DpnII GATC 3 cut(s) 504, 595, 661
DraI TTTAAA 1 cut(s) 640
Eam1104I CTCTTC 3 cut(s) 756, 771, 804
EarI CTCTTC 3 cut(s) 756, 771, 804
Eco130I CCWWGG 1 cut(s) 258
Eco32I GATATC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 217
Eco57I CTGAAG 2 cut(s) 206, 737
Eco88I CYCGRG 1 cut(s) 537
EcoO109I RGGNCCY 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 781
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 6 cut(s) 126, 262, 603, 686, 710, 743
FatI CATG 6 cut(s) 122, 258, 599, 682, 706, 739
FauNDI CATATG 1 cut(s) 559
FblI GTMKAC 1 cut(s) 245
Fnu4HI GCNGC 2 cut(s) 320, 891
FokI GGATG 2 cut(s) 130, 707
Fsp4HI GCNGC 2 cut(s) 320, 891
FspBI CTAG 3 cut(s) 192, 332, 459
GluI GCNGC 2 cut(s) 320, 891
GsuI CTGGAG 1 cut(s) 870
HapII CCGG 1 cut(s) 115
Hin1II CATG 6 cut(s) 126, 262, 603, 686, 710, 743
HinfI GANTC 5 cut(s) 211, 478, 540, 676, 838
HpaII CCGG 1 cut(s) 115
HphI GGTGA 2 cut(s) 251, 425
Hpy166II GTNNAC 3 cut(s) 111, 246, 565
Hpy188I TCNGA 3 cut(s) 229, 669, 802
Hpy188III TCNNGA 4 cut(s) 401, 707, 740, 788
Hpy8I GTNNAC 3 cut(s) 111, 246, 565
Hpy99I CGWCG 1 cut(s) 19
HpyAV CCTTC 5 cut(s) 230, 465, 756, 854, 861
HpyCH4V TGCA 2 cut(s) 111, 431
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 896
HpyF3I CTNAG 2 cut(s) 666, 786
Hsp92II CATG 6 cut(s) 126, 262, 603, 686, 710, 743
Kzo9I GATC 3 cut(s) 504, 595, 661
LmnI GCTCC 2 cut(s) 517, 898
Lsp1109I GCAGC 2 cut(s) 331, 877
LweI GCATC 1 cut(s) 20
MaeI CTAG 3 cut(s) 192, 332, 459
MaeIII GTNAC 2 cut(s) 91, 873
MalI GATC 3 cut(s) 506, 597, 663
MboI GATC 3 cut(s) 504, 595, 661
MboII GAAGA 7 cut(s) 36, 151, 359, 685, 743, 788, 821
MhlI GDGCHC 1 cut(s) 113
MluCI AATT 6 cut(s) 152, 346, 469, 526, 781, 804
MlyI GAGTC 3 cut(s) 472, 549, 670
MmeI TCCRAC 1 cut(s) 339
MnlI CCTC 9 cut(s) 70, 385, 408, 492, 546, 675, 709, 805, 811
MroXI GAANNNNTTC 1 cut(s) 366
MseI TTAA 3 cut(s) 444, 468, 639
MslI CAYNNNNRTG 2 cut(s) 116, 711
MspA1I CMGCKG 1 cut(s) 890
MspI CCGG 1 cut(s) 115
MspR9I CCNGG 1 cut(s) 115
Mva1269I GAATGC 1 cut(s) 285
MwoI GCNNNNNNNGC 2 cut(s) 170, 896
NciI CCSGG 1 cut(s) 115
NcoI CCATGG 1 cut(s) 258
NdeI CATATG 1 cut(s) 559
NdeII GATC 3 cut(s) 504, 595, 661
NlaIII CATG 6 cut(s) 126, 262, 603, 686, 710, 743
NlaIV GGNNCC 1 cut(s) 534
NmuCI GTSAC 1 cut(s) 873
NspV TTCGAA 1 cut(s) 47
PaeR7I CTCGAG 1 cut(s) 537
PagI TCATGA 2 cut(s) 706, 739
PctI GAATGC 1 cut(s) 285
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 2 cut(s) 211, 838
PkrI GCNGC 2 cut(s) 321, 892
PleI GAGTC 3 cut(s) 472, 548, 670
PpsI GAGTC 3 cut(s) 472, 548, 670
PpuMI RGGWCCY 1 cut(s) 217
PshBI ATTAAT 1 cut(s) 468
Psp5II RGGWCCY 1 cut(s) 217
PspN4I GGNNCC 1 cut(s) 534
PspPI GGNCC 1 cut(s) 217
PspPPI RGGWCCY 1 cut(s) 217
PspXI VCTCGAGB 1 cut(s) 537
PvuII CAGCTG 1 cut(s) 890
RsaI GTAC 3 cut(s) 200, 564, 831
RsaNI GTAC 3 cut(s) 199, 563, 830
RseI CAYNNNNRTG 2 cut(s) 116, 711
SaqAI TTAA 3 cut(s) 444, 468, 639
SatI GCNGC 2 cut(s) 320, 891
Sau3AI GATC 3 cut(s) 504, 595, 661
Sau96I GGNCC 1 cut(s) 217
SchI GAGTC 3 cut(s) 472, 549, 670
ScrFI CCNGG 1 cut(s) 115
SduI GDGCHC 1 cut(s) 113
SetI ASST 8 cut(s) 219, 499, 526, 538, 739, 770, 816, 892
SfaNI GCATC 1 cut(s) 20
Sfr274I CTCGAG 1 cut(s) 537
SfuI TTCGAA 1 cut(s) 47
SinI GGWCC 1 cut(s) 217
SlaI CTCGAG 1 cut(s) 537
SmiMI CAYNNNNRTG 2 cut(s) 116, 711
SmlI CTYRAG 2 cut(s) 279, 537
SmoI CTYRAG 2 cut(s) 279, 537
Sse9I AATT 6 cut(s) 152, 346, 469, 526, 781, 804
SsiI CCGC 3 cut(s) 317, 448, 632
SspI AATATT 1 cut(s) 407
SspMI CTAG 3 cut(s) 192, 332, 459
StyD4I CCNGG 1 cut(s) 113
StyI CCWWGG 1 cut(s) 258
TaqI TCGA 2 cut(s) 47, 538
TasI AATT 6 cut(s) 152, 346, 469, 526, 781, 804
TatI WGTACW 1 cut(s) 562
TfiI GAWTC 2 cut(s) 211, 838
Tru1I TTAA 3 cut(s) 444, 468, 639
Tru9I TTAA 3 cut(s) 444, 468, 639
TseFI GTSAC 1 cut(s) 873
TseI GCWGC 2 cut(s) 319, 890
Tsp45I GTSAC 1 cut(s) 873
TspDTI ATGAA 4 cut(s) 152, 404, 695, 723
VneI GTGCAC 1 cut(s) 109
VpaK11BI GGWCC 1 cut(s) 217
VspI ATTAAT 1 cut(s) 468
XapI RAATTY 1 cut(s) 781
XhoI CTCGAG 1 cut(s) 537
XmiI GTMKAC 1 cut(s) 245
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 3 cut(s) 192, 332, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.