Reverse transcriptase (RNA-dependent DNA polymerase)
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 4 cut(s) | 307, 332, 345, 796 |
| AcsI | RAATTY | 1 cut(s) | 12 |
| AdeI | CACNNNGTG | 1 cut(s) | 206 |
| AfaI | GTAC | 1 cut(s) | 553 |
| AgsI | TTSAA | 4 cut(s) | 62, 98, 696, 730 |
| AleI | CACNNNNGTG | 1 cut(s) | 242 |
| AluBI | AGCT | 3 cut(s) | 172, 605, 715 |
| AluI | AGCT | 3 cut(s) | 172, 605, 715 |
| Alw21I | GWGCWC | 1 cut(s) | 541 |
| Alw26I | GTCTC | 2 cut(s) | 254, 665 |
| AlwI | GGATC | 4 cut(s) | 307, 332, 345, 796 |
| Ama87I | CYCGRG | 1 cut(s) | 428 |
| AoxI | GGCC | 2 cut(s) | 178, 648 |
| ApeKI | GCWGC | 1 cut(s) | 605 |
| ApoI | RAATTY | 1 cut(s) | 12 |
| AspS9I | GGNCC | 1 cut(s) | 178 |
| AvaI | CYCGRG | 1 cut(s) | 428 |
| BamHI | GGATCC | 1 cut(s) | 337 |
| BanII | GRGCYC | 2 cut(s) | 294, 726 |
| Bbv12I | GWGCWC | 1 cut(s) | 541 |
| BbvI | GCAGC | 1 cut(s) | 592 |
| BccI | CCATC | 3 cut(s) | 280, 328, 717 |
| BceAI | ACGGC | 1 cut(s) | 447 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 287, 321, 419, 453 |
| BciVI | GTATCC | 2 cut(s) | 826, 847 |
| BclI | TGATCA | 1 cut(s) | 750 |
| BcoDI | GTCTC | 2 cut(s) | 254, 665 |
| BfaI | CTAG | 2 cut(s) | 405, 719 |
| BfmI | CTRYAG | 1 cut(s) | 477 |
| BfuI | GTATCC | 2 cut(s) | 826, 847 |
| BisI | GCNGC | 1 cut(s) | 606 |
| BlpI | GCTNAGC | 1 cut(s) | 293 |
| BlsI | GCNGC | 1 cut(s) | 607 |
| BmeT110I | CYCGRG | 1 cut(s) | 428 |
| BmgT120I | GGNCC | 1 cut(s) | 178 |
| BmiI | GGNNCC | 1 cut(s) | 339 |
| BmsI | GCATC | 3 cut(s) | 31, 187, 443 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 528, 560 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 293 |
| BpuEI | CTTGAG | 2 cut(s) | 158, 530 |
| BsaBI | GATNNNNATC | 1 cut(s) | 471 |
| BsaJI | CCNNGG | 1 cut(s) | 429 |
| Bse1I | ACTGG | 1 cut(s) | 569 |
| Bse3DI | GCAATG | 4 cut(s) | 31, 374, 375, 615 |
| Bse8I | GATNNNNATC | 1 cut(s) | 471 |
| BseDI | CCNNGG | 1 cut(s) | 429 |
| BseGI | GGATG | 4 cut(s) | 155, 193, 202, 339 |
| BseJI | GATNNNNATC | 1 cut(s) | 471 |
| BseMI | GCAATG | 4 cut(s) | 31, 374, 375, 615 |
| BseMII | CTCAG | 2 cut(s) | 307, 806 |
| BseNI | ACTGG | 1 cut(s) | 569 |
| BseRI | GAGGAG | 2 cut(s) | 643, 716 |
| BseXI | GCAGC | 1 cut(s) | 592 |
| BshFI | GGCC | 2 cut(s) | 180, 650 |
| BsiHKAI | GWGCWC | 1 cut(s) | 541 |
| BsiHKCI | CYCGRG | 1 cut(s) | 428 |
| BslFI | GGGAC | 1 cut(s) | 585 |
| BsmAI | GTCTC | 2 cut(s) | 254, 665 |
| BsmBI | CGTCTC | 1 cut(s) | 254 |
| BsmFI | GGGAC | 1 cut(s) | 585 |
| BsnI | GGCC | 2 cut(s) | 180, 650 |
| BsoBI | CYCGRG | 1 cut(s) | 428 |
| Bsp1286I | GDGCHC | 3 cut(s) | 294, 541, 726 |
| Bsp143I | GATC | 4 cut(s) | 312, 337, 750, 801 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 293 |
| BspANI | GGCC | 2 cut(s) | 180, 650 |
| BspCNI | CTCAG | 2 cut(s) | 306, 807 |
| BspHI | TCATGA | 1 cut(s) | 382 |
| BspLI | GGNNCC | 1 cut(s) | 339 |
| BspPI | GGATC | 4 cut(s) | 307, 332, 345, 796 |
| BsrDI | GCAATG | 4 cut(s) | 31, 374, 375, 615 |
| BsrI | ACTGG | 1 cut(s) | 569 |
| BssECI | CCNNGG | 1 cut(s) | 429 |
| BssMI | GATC | 4 cut(s) | 312, 337, 750, 801 |
| Bst4CI | ACNGT | 2 cut(s) | 94, 102 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 206 |
| BstC8I | GCNNGC | 1 cut(s) | 744 |
| BstDEI | CTNAG | 2 cut(s) | 293, 815 |
| BstF5I | GGATG | 4 cut(s) | 155, 193, 202, 339 |
| BstKTI | GATC | 4 cut(s) | 315, 340, 753, 804 |
| BstMAI | GTCTC | 2 cut(s) | 254, 665 |
| BstMBI | GATC | 4 cut(s) | 312, 337, 750, 801 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 64, 206, 721 |
| BstSFI | CTRYAG | 1 cut(s) | 477 |
| BstV1I | GCAGC | 1 cut(s) | 592 |
| BstX2I | RGATCY | 1 cut(s) | 337 |
| BstYI | RGATCY | 1 cut(s) | 337 |
| BsuI | GTATCC | 2 cut(s) | 826, 847 |
| BsuRI | GGCC | 2 cut(s) | 180, 650 |
| BtsCI | GGATG | 4 cut(s) | 155, 193, 202, 339 |
| BtsI | GCAGTG | 1 cut(s) | 28 |
| BtsIMutI | CAGTG | 2 cut(s) | 28, 453 |
| Cac8I | GCNNGC | 1 cut(s) | 744 |
| CciI | TCATGA | 1 cut(s) | 382 |
| Cfr13I | GGNCC | 1 cut(s) | 178 |
| CseI | GACGC | 2 cut(s) | 271, 368 |
| Csp6I | GTAC | 1 cut(s) | 552 |
| CviAII | CATG | 5 cut(s) | 125, 274, 383, 644, 841 |
| CviQI | GTAC | 1 cut(s) | 552 |
| DdeI | CTNAG | 2 cut(s) | 293, 815 |
| DpnI | GATC | 4 cut(s) | 314, 339, 752, 803 |
| DpnII | GATC | 4 cut(s) | 312, 337, 750, 801 |
| DraIII | CACNNNGTG | 1 cut(s) | 206 |
| Eco24I | GRGCYC | 2 cut(s) | 294, 726 |
| Eco88I | CYCGRG | 1 cut(s) | 428 |
| EcoO109I | RGGNCCY | 1 cut(s) | 178 |
| EcoT38I | GRGCYC | 2 cut(s) | 294, 726 |
| Esp3I | CGTCTC | 1 cut(s) | 254 |
| FaeI | CATG | 5 cut(s) | 128, 277, 386, 647, 844 |
| FaqI | GGGAC | 1 cut(s) | 585 |
| FatI | CATG | 5 cut(s) | 124, 273, 382, 643, 840 |
| FauNDI | CATATG | 2 cut(s) | 53, 364 |
| FbaI | TGATCA | 1 cut(s) | 750 |
| Fnu4HI | GCNGC | 1 cut(s) | 606 |
| FokI | GGATG | 4 cut(s) | 142, 200, 209, 346 |
| FriOI | GRGCYC | 2 cut(s) | 294, 726 |
| Fsp4HI | GCNGC | 1 cut(s) | 606 |
| FspBI | CTAG | 2 cut(s) | 405, 719 |
| GluI | GCNGC | 1 cut(s) | 606 |
| HaeIII | GGCC | 2 cut(s) | 180, 650 |
| HgaI | GACGC | 2 cut(s) | 271, 368 |
| Hin1II | CATG | 5 cut(s) | 128, 277, 386, 647, 844 |
| HinfI | GANTC | 1 cut(s) | 75 |
| Hpy166II | GTNNAC | 2 cut(s) | 355, 552 |
| Hpy188I | TCNGA | 1 cut(s) | 317 |
| Hpy188III | TCNNGA | 4 cut(s) | 112, 283, 383, 727 |
| Hpy8I | GTNNAC | 2 cut(s) | 355, 552 |
| HpyAV | CCTTC | 5 cut(s) | 187, 241, 562, 608, 818 |
| HpyCH4III | ACNGT | 2 cut(s) | 94, 102 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 64, 206, 721 |
| HpyF3I | CTNAG | 2 cut(s) | 293, 815 |
| Hsp92II | CATG | 5 cut(s) | 128, 277, 386, 647, 844 |
| Ksp22I | TGATCA | 1 cut(s) | 750 |
| Kzo9I | GATC | 4 cut(s) | 312, 337, 750, 801 |
| LpnPI | CCDG | 3 cut(s) | 3, 550, 756 |
| Lsp1109I | GCAGC | 1 cut(s) | 592 |
| LweI | GCATC | 3 cut(s) | 31, 187, 443 |
| MaeI | CTAG | 2 cut(s) | 405, 719 |
| MaeIII | GTNAC | 2 cut(s) | 151, 227 |
| MalI | GATC | 4 cut(s) | 314, 339, 752, 803 |
| MboI | GATC | 4 cut(s) | 312, 337, 750, 801 |
| MboII | GAAGA | 1 cut(s) | 494 |
| MflI | RGATCY | 1 cut(s) | 337 |
| MhlI | GDGCHC | 3 cut(s) | 294, 541, 726 |
| MluCI | AATT | 5 cut(s) | 12, 62, 301, 529, 775 |
| MslI | CAYNNNNRTG | 3 cut(s) | 129, 242, 579 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 64, 206, 721 |
| NdeI | CATATG | 2 cut(s) | 53, 364 |
| NdeII | GATC | 4 cut(s) | 312, 337, 750, 801 |
| NlaIII | CATG | 5 cut(s) | 128, 277, 386, 647, 844 |
| NlaIV | GGNNCC | 1 cut(s) | 339 |
| NmuCI | GTSAC | 2 cut(s) | 151, 227 |
| OliI | CACNNNNGTG | 1 cut(s) | 242 |
| PagI | TCATGA | 1 cut(s) | 382 |
| PfeI | GAWTC | 1 cut(s) | 75 |
| PkrI | GCNGC | 1 cut(s) | 607 |
| PspN4I | GGNNCC | 1 cut(s) | 339 |
| PspPI | GGNCC | 1 cut(s) | 178 |
| PsuI | RGATCY | 1 cut(s) | 337 |
| RsaI | GTAC | 1 cut(s) | 553 |
| RsaNI | GTAC | 1 cut(s) | 552 |
| RseI | CAYNNNNRTG | 3 cut(s) | 129, 242, 579 |
| SatI | GCNGC | 1 cut(s) | 606 |
| Sau3AI | GATC | 4 cut(s) | 312, 337, 750, 801 |
| Sau96I | GGNCC | 1 cut(s) | 178 |
| SduI | GDGCHC | 3 cut(s) | 294, 541, 726 |
| SetI | ASST | 9 cut(s) | 174, 402, 546, 557, 581, 607, 640, 717, 863 |
| SfaNI | GCATC | 3 cut(s) | 31, 187, 443 |
| SfcI | CTRYAG | 1 cut(s) | 477 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 129, 242, 579 |
| SmlI | CTYRAG | 2 cut(s) | 173, 545 |
| SmoI | CTYRAG | 2 cut(s) | 173, 545 |
| Sse9I | AATT | 5 cut(s) | 12, 62, 301, 529, 775 |
| SspI | AATATT | 1 cut(s) | 587 |
| SspMI | CTAG | 2 cut(s) | 405, 719 |
| TaaI | ACNGT | 2 cut(s) | 94, 102 |
| TaqI | TCGA | 2 cut(s) | 5, 111 |
| TaqII | GACCGA | 1 cut(s) | 139 |
| TasI | AATT | 5 cut(s) | 12, 62, 301, 529, 775 |
| TfiI | GAWTC | 1 cut(s) | 75 |
| TscAI | CASTG | 2 cut(s) | 28, 453 |
| TseFI | GTSAC | 2 cut(s) | 151, 227 |
| TseI | GCWGC | 1 cut(s) | 605 |
| Tsp45I | GTSAC | 2 cut(s) | 151, 227 |
| TspDTI | ATGAA | 5 cut(s) | 20, 132, 290, 597, 692 |
| TspRI | CASTG | 2 cut(s) | 28, 453 |
| XapI | RAATTY | 1 cut(s) | 12 |
| XspI | CTAG | 2 cut(s) | 405, 719 |