Rw0G012970

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00575
OG0000106
Physical Location & Seq
Reverse (-)
18318 .. 21123
2806 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G012970.1

Sequence Viewer

Length: 849 bp
ATGTCAAAAGAAATTTCTGGCAGTGTCATCCATTGCAAGGATGCAAGAAGCATGTGGCTTGAATTGCAGGAAAGGTTCTCACATACAAACACCGTGCAACTTTTCCATATAGAAAACGCCATTCATGATTGTGAACAAGGCGGTACCTCAGTGACATCTTTCTTCACCAAACTCAAGACTCTCTGGGATGAGAAAGATGCCTTATGTGAAATTCCTCCATGCAGTTGCGAAGCAGCCAAGGAAATCAAGGATTACTTGGAGACACAAAAGACAATGAAATTTTTGATGGGTCTCAATGACAGTTATGCCACGATTCGAAGCAATATTGTTGGGATGGATCCTCTTCCGAATGTGAACAAGGCTTACTCCATGGCACTTCGACATGAAAAGCAAGCAGAGGTGTCAAGTGGCAAGATGTCTACTCAGCCGGAGGCAGCTGCCTTTGCTGTGAAGAGAGCCAACCGTGAGTTTGACCTTGGAGAAAGTGAGACTAAATGTGAAAAATGCAACAAAAATAATCACAGCACAAAGAATTGCAGGGCACACCTCAAGTGTACATTTTGCAATTGGCAAGGGCACACTTACGAGTACTGCCGCAAGAGGAAGGCTGCCATGGAGGGAGGACAAGTGCGTGCGAAAGGAAATCATGTGCTCTCCATGCATGATAAGCAAGAAGCTGGACCCAGTTTTCCATTTTCACAAGAAGAGTGTCAACAGCTTATGAACCTCTTAAACAAAAACAAACCTGCCACAGCAAATCAAGTTGGTAATGTTCCAAACTTTGATGAAATCTCAGTCAAATTGAAGGGCATGATTATAGTGAGACTTTCGCCCCTGTTGCAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2

Protein Analysis

282

Amino Acids

31.83

Weight (kDa)

8.39

Isoelectric Point (pI)

51.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000106)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20980 AT3G21000
fragaria_vesca FvH4_1g15931 FvH4_1g15931 FvH4_1g18381 FvH4_1g18382 FvH4_1g22571 FvH4_1g24950 FvH4_1g29372 FvH4_2g03582 FvH4_2g09851 FvH4_2g13112 FvH4_2g20843 FvH4_2g22701 FvH4_2g22702 FvH4_2g23403 FvH4_2g40201 FvH4_3g03542 FvH4_3g03543 FvH4_3g04761 FvH4_3g10464 FvH4_3g12741 FvH4_3g15781 FvH4_3g30981 FvH4_4g00985 FvH4_4g07035 FvH4_4g08192 FvH4_4g08211 FvH4_4g08553 FvH4_4g14242 FvH4_4g14243 FvH4_4g14641 FvH4_4g14843 FvH4_4g14844 FvH4_4g15061 FvH4_4g16281 FvH4_4g19152 FvH4_4g26451 FvH4_5g06442 FvH4_5g22521 FvH4_5g24301 FvH4_5g38641 FvH4_6g02393 FvH4_6g12782 FvH4_6g13871 FvH4_6g19391 FvH4_6g20105 FvH4_6g21292 FvH4_6g23581 FvH4_6g29153 FvH4_6g29691 FvH4_6g30871 FvH4_6g33351 FvH4_6g37042 FvH4_6g38781 FvH4_6g40340 FvH4_7g11221 FvH4_7g23352 FvH4_7g23352 FvH4_7g31212 FvH4_7g32172 FvH4_7g32173 FvH4_7g33701
malus_domestica MD03G1006900.v1.1 MD04G1039100.v1.1 MD04G1239100.v1.1 MD05G1048700.v1.1 MD08G1001800.v1.1 MD09G1165800.v1.1 MD11G1065200.v1.1 MD11G1138200.v1.1 MD14G1033300.v1.1 MD14G1173900.v1.1 MD15G1218200.v1.1 MD16G1034000.v1.1
prunus_persica Prupe.1G219500_v2.0.a1 Prupe.2G169400_v2.0.a1 Prupe.4G109700_v2.0.a1 Prupe.7G022000_v2.0.a1
pyrus_communis pycom01g02000 pycom02g13350 pycom03g01010 pycom04g05000 pycom04g06100 pycom05g05580 pycom05g09950 pycom05g13280 pycom05g26610 pycom06g05940 pycom06g07760 pycom07g06120 pycom07g24780 pycom08g08820 pycom09g03600 pycom09g07200 pycom10g01280 pycom10g06730 pycom10g08410 pycom10g25520 pycom11g00470 pycom11g05250 pycom11g25180 pycom12g08050 pycom13g14760 pycom13g28170 pycom15g14310 pycom15g19880 pycom15g24230 pycom15g33640 pycom16g02150 pycom16g16240 pycom16g20050 pycom16g21090 pycom17g06100
rosa_chinensis RchiOBHm_Chr1g0322161 RchiOBHm_Chr1g0326341 RchiOBHm_Chr1g0357701 RchiOBHm_Chr1g0379861 RchiOBHm_Chr1g0383421 RchiOBHm_Chr2g0093981 RchiOBHm_Chr2g0139051 RchiOBHm_Chr2g0145501 RchiOBHm_Chr3g0450971 RchiOBHm_Chr3g0451441 RchiOBHm_Chr3g0457411 RchiOBHm_Chr4g0436261 RchiOBHm_Chr5g0051431 RchiOBHm_Chr5g0064031 RchiOBHm_Chr5g0073141 RchiOBHm_Chr5g0080061 RchiOBHm_Chr7g0200561 RchiOBHm_Chr7g0235911
rosa_laevigata RLG00000000145 RLG00000000429 RLG00000020339 RLG00000035212
rosa_multiflora Rmu_co7977988.1_g000001 Rmu_co8037866.1_g000001 Rmu_co8243895.1_g000001 Rmu_co8363787.1_g000001 Rmu_co8381003.1_g000001 Rmu_sc0000263.1_g000006 Rmu_sc0000349.1_g000002 Rmu_sc0000455.1_g000031 Rmu_sc0000936.1_g000022 Rmu_sc0001035.1_g000023 Rmu_sc0001169.1_g000019 Rmu_sc0001250.1_g000026 Rmu_sc0002200.1_g000051 Rmu_sc0002230.1_g000027 Rmu_sc0002591.1_g000039 Rmu_sc0003182.1_g000003 Rmu_sc0003441.1_g000068 Rmu_sc0003722.1_g000018 Rmu_sc0003764.1_g000007 Rmu_sc0006014.1_g000016 Rmu_sc0012582.1_g000002 Rmu_sc0013519.1_g000002 Rmu_sc0014550.1_g000005 Rmu_sc0014857.1_g000007 Rmu_sc0019855.1_g000001 Rmu_sc0030839.1_g000001 Rmu_sc0031308.1_g000001
rosa_roxburghii Rroxscaffold_1G00011290 Rroxscaffold_1G00041340 Rroxscaffold_1G00053780 Rroxscaffold_1G00059130 Rroxscaffold_1G00061580 Rroxscaffold_2G00080940 Rroxscaffold_2G00109540 Rroxscaffold_3G00228400 Rroxscaffold_3G00237710 Rroxscaffold_4G00285210 Rroxscaffold_4G00299600 Rroxscaffold_4G00324940 Rroxscaffold_4G00330580 Rroxscaffold_5G00333000 Rroxscaffold_5G00347920 Rroxscaffold_5G00375310 Rroxscaffold_6G00394760 Rroxscaffold_6G00406260 Rroxscaffold_7G00165690 Rroxscaffold_7G00187680
rosa_rugosa Rorug01G0050200 Rorug02G0273800 Rorug06G0490600 Rorug06G0490600 Rorug06G0490700
rosa_samantha Rh1AG348400 Rh5AG222600 Rh6DG000500 Rh6DG194500 Rh7BG452700
rosa_wichuraiana Rw0G003060 Rw0G010530 Rw0G012970 Rw0G022560 Rw0G023390 Rw1G001270 Rw1G001530 Rw1G006070 Rw1G008210 Rw1G022090 Rw1G023320 Rw2G003790 Rw2G004140 Rw2G005280 Rw2G006460 Rw2G019620 Rw2G019840 Rw2G022530 Rw2G023280 Rw2G024990 Rw2G042380 Rw2G051390 Rw3G001640 Rw3G016720 Rw3G017100 Rw3G020890 Rw3G020900 Rw3G021100 Rw4G004820 Rw4G014950 Rw4G030920 Rw4G031400 Rw5G008500 Rw5G017950 Rw5G020170 Rw5G030160 Rw5G033050 Rw5G034640 Rw5G043920 Rw6G005060 Rw6G005540 Rw6G006720 Rw6G008460 Rw6G008670 Rw6G014780 Rw6G019100 Rw6G019350 Rw6G026310 Rw7G016810 Rw7G019180 Rw7G022890 Rw7G025880 Rw7G026390 Rw7G028340 Rw7G034850 Rw7G035170 Rw7G035420 Rw7G037990 Rw7G041560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 754
Acc65I GGTACC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 419
AciI CCGC 2 cut(s) 141, 595
AclWI GGATC 2 cut(s) 332, 345
AcsI RAATTY 3 cut(s) 12, 210, 278
AfaI GTAC 3 cut(s) 145, 556, 590
AfiI CCNNNNNNNGG 1 cut(s) 37
AgsI TTSAA 2 cut(s) 62, 805
AluBI AGCT 3 cut(s) 437, 677, 718
AluI AGCT 3 cut(s) 437, 677, 718
Alw21I GWGCWC 1 cut(s) 654
Alw26I GTCTC 4 cut(s) 254, 296, 482, 817
AlwI GGATC 2 cut(s) 332, 345
ApeKI GCWGC 4 cut(s) 233, 434, 437, 608
ApoI RAATTY 3 cut(s) 12, 210, 278
Asp718I GGTACC 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 680
AsuHPI GGTGA 1 cut(s) 157
AsuII TTCGAA 1 cut(s) 316
AvaII GGWCC 1 cut(s) 680
BaeGI GKGCMC 2 cut(s) 544, 579
BaeI ACNNNNGTAYC 2 cut(s) 135, 168
BamHI GGATCC 1 cut(s) 337
BanI GGYRCC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 654
BbvI GCAGC 4 cut(s) 245, 424, 446, 595
BccI CCATC 2 cut(s) 280, 328
BcoDI GTCTC 4 cut(s) 254, 296, 482, 817
BfuAI ACCTGC 1 cut(s) 754
BisI GCNGC 5 cut(s) 234, 435, 438, 595, 609
BlsI GCNGC 5 cut(s) 235, 436, 439, 596, 610
BmcAI AGTACT 1 cut(s) 590
Bme18I GGWCC 1 cut(s) 680
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 3 cut(s) 145, 339, 682
BmrI ACTGGG 1 cut(s) 678
BmsI GCATC 2 cut(s) 31, 187
BmuI ACTGGG 1 cut(s) 678
Bpu14I TTCGAA 1 cut(s) 316
BpuEI CTTGAG 2 cut(s) 158, 533
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 4 cut(s) 237, 369, 475, 612
BsaXI ACNNNNNCTCC 2 cut(s) 612, 642
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 684
Bse3DI GCAATG 1 cut(s) 31
BseDI CCNNGG 4 cut(s) 237, 369, 475, 612
BseGI GGATG 4 cut(s) 27, 46, 193, 339
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMI GCAATG 1 cut(s) 31
BseMII CTCAG 3 cut(s) 162, 437, 807
BseNI ACTGG 1 cut(s) 684
BseSI GKGCMC 2 cut(s) 544, 579
BseXI GCAGC 4 cut(s) 245, 424, 446, 595
BshNI GGYRCC 1 cut(s) 143
BsiHKAI GWGCWC 1 cut(s) 654
BsiSI CCGG 1 cut(s) 428
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 4 cut(s) 254, 296, 482, 817
Bso31I GGTCTC 1 cut(s) 296
Bsp119I TTCGAA 1 cut(s) 316
Bsp1286I GDGCHC 3 cut(s) 544, 579, 654
Bsp1407I TGTACA 1 cut(s) 554
Bsp143I GATC 1 cut(s) 337
Bsp19I CCATGG 2 cut(s) 369, 612
BspACI CCGC 2 cut(s) 141, 595
BspCNI CTCAG 3 cut(s) 161, 436, 806
BspHI TCATGA 1 cut(s) 124
BspLI GGNNCC 3 cut(s) 145, 339, 682
BspMI ACCTGC 1 cut(s) 754
BspPI GGATC 2 cut(s) 332, 345
BspT104I TTCGAA 1 cut(s) 316
BspT107I GGYRCC 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 296
BsrDI GCAATG 1 cut(s) 31
BsrGI TGTACA 1 cut(s) 554
BsrI ACTGG 1 cut(s) 684
BssECI CCNNGG 4 cut(s) 237, 369, 475, 612
BssMI GATC 1 cut(s) 337
BssT1I CCWWGG 4 cut(s) 237, 369, 475, 612
Bst4CI ACNGT 3 cut(s) 94, 302, 464
Bst6I CTCTTC 3 cut(s) 348, 446, 699
BstAUI TGTACA 1 cut(s) 554
BstBI TTCGAA 1 cut(s) 316
BstC8I GCNNGC 2 cut(s) 393, 633
BstDEI CTNAG 3 cut(s) 148, 423, 793
BstDSI CCRYGG 2 cut(s) 369, 612
BstF5I GGATG 4 cut(s) 27, 46, 193, 339
BstKTI GATC 1 cut(s) 340
BstMAI GTCTC 4 cut(s) 254, 296, 482, 817
BstMBI GATC 1 cut(s) 337
BstMWI GCNNNNNNNGC 5 cut(s) 64, 443, 658, 667, 838
BstNSI RCATGY 1 cut(s) 55
BstSLI GKGCMC 2 cut(s) 544, 579
BstV1I GCAGC 4 cut(s) 245, 424, 446, 595
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BtgI CCRYGG 2 cut(s) 369, 612
BtsCI GGATG 4 cut(s) 27, 46, 193, 339
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 2 cut(s) 28, 156
BveI ACCTGC 1 cut(s) 754
Cac8I GCNNGC 2 cut(s) 393, 633
CciI TCATGA 1 cut(s) 124
Cfr13I GGNCC 1 cut(s) 680
Csp6I GTAC 3 cut(s) 144, 555, 589
CviJI RGCY 9 cut(s) 58, 236, 362, 427, 437, 458, 608, 677, 718
CviKI_1 RGCY 9 cut(s) 58, 236, 362, 427, 437, 458, 608, 677, 718
CviQI GTAC 3 cut(s) 144, 555, 589
DdeI CTNAG 3 cut(s) 148, 423, 793
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
Eam1104I CTCTTC 3 cut(s) 348, 446, 699
EarI CTCTTC 3 cut(s) 348, 446, 699
Eco130I CCWWGG 4 cut(s) 237, 369, 475, 612
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 680
EcoT14I CCWWGG 4 cut(s) 237, 369, 475, 612
EcoT22I ATGCAT 1 cut(s) 663
ErhI CCWWGG 4 cut(s) 237, 369, 475, 612
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FblI GTMKAC 1 cut(s) 419
Fnu4HI GCNGC 5 cut(s) 234, 435, 438, 595, 609
FokI GGATG 4 cut(s) 14, 53, 200, 346
Fsp4HI GCNGC 5 cut(s) 234, 435, 438, 595, 609
GluI GCNGC 5 cut(s) 234, 435, 438, 595, 609
HapII CCGG 1 cut(s) 428
HincII GTYRAC 1 cut(s) 713
HindII GTYRAC 1 cut(s) 713
HinfI GANTC 2 cut(s) 178, 313
HpaII CCGG 1 cut(s) 428
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 5 cut(s) 134, 355, 420, 555, 713
Hpy188I TCNGA 1 cut(s) 348
Hpy188III TCNNGA 2 cut(s) 125, 175
Hpy8I GTNNAC 5 cut(s) 134, 355, 420, 555, 713
HpyAV CCTTC 2 cut(s) 598, 799
HpyCH4III ACNGT 3 cut(s) 94, 302, 464
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 443, 658, 667, 838
HpyF3I CTNAG 3 cut(s) 148, 423, 793
KpnI GGTACC 1 cut(s) 147
Kzo9I GATC 1 cut(s) 337
LpnPI CCDG 8 cut(s) 3, 53, 169, 441, 523, 663, 697, 759
Lsp1109I GCAGC 4 cut(s) 245, 424, 446, 595
LweI GCATC 2 cut(s) 31, 187
MaeIII GTNAC 1 cut(s) 151
MalI GATC 1 cut(s) 339
MboI GATC 1 cut(s) 337
MboII GAAGA 4 cut(s) 154, 335, 463, 716
MfeI CAATTG 1 cut(s) 565
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 3 cut(s) 544, 579, 654
MluCI AATT 8 cut(s) 12, 62, 210, 278, 532, 565, 800, 844
MlyI GAGTC 1 cut(s) 172
Mph1103I ATGCAT 1 cut(s) 663
MseI TTAA 1 cut(s) 731
MslI CAYNNNNRTG 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 1 cut(s) 428
MunI CAATTG 1 cut(s) 565
MwoI GCNNNNNNNGC 5 cut(s) 64, 443, 658, 667, 838
NcoI CCATGG 2 cut(s) 369, 612
NdeII GATC 1 cut(s) 337
NlaIV GGNNCC 3 cut(s) 145, 339, 682
NmuCI GTSAC 1 cut(s) 151
NsiI ATGCAT 1 cut(s) 663
NspI RCATGY 1 cut(s) 55
NspV TTCGAA 1 cut(s) 316
PagI TCATGA 1 cut(s) 124
PfeI GAWTC 1 cut(s) 313
PkrI GCNGC 5 cut(s) 235, 436, 439, 596, 610
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PspN4I GGNNCC 3 cut(s) 145, 339, 682
PspPI GGNCC 1 cut(s) 680
PsrI GAACNNNNNNTAC 2 cut(s) 347, 379
PsuI RGATCY 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 3 cut(s) 145, 556, 590
RsaNI GTAC 3 cut(s) 144, 555, 589
RseI CAYNNNNRTG 1 cut(s) 129
SaqAI TTAA 1 cut(s) 731
SatI GCNGC 5 cut(s) 234, 435, 438, 595, 609
Sau3AI GATC 1 cut(s) 337
Sau96I GGNCC 1 cut(s) 680
ScaI AGTACT 1 cut(s) 590
SchI GAGTC 1 cut(s) 172
SduI GDGCHC 3 cut(s) 544, 579, 654
SfaNI GCATC 2 cut(s) 31, 187
SfuI TTCGAA 1 cut(s) 316
SinI GGWCC 1 cut(s) 680
SmiMI CAYNNNNRTG 1 cut(s) 129
SmlI CTYRAG 2 cut(s) 173, 548
SmoI CTYRAG 2 cut(s) 173, 548
Sse9I AATT 8 cut(s) 12, 62, 210, 278, 532, 565, 800, 844
SsiI CCGC 2 cut(s) 141, 595
SspI AATATT 1 cut(s) 325
StyI CCWWGG 4 cut(s) 237, 369, 475, 612
TaaI ACNGT 3 cut(s) 94, 302, 464
TaqI TCGA 2 cut(s) 316, 379
TasI AATT 8 cut(s) 12, 62, 210, 278, 532, 565, 800, 844
TatI WGTACW 2 cut(s) 554, 588
TauI GCSGC 1 cut(s) 597
TfiI GAWTC 1 cut(s) 313
Tru1I TTAA 1 cut(s) 731
Tru9I TTAA 1 cut(s) 731
TscAI CASTG 2 cut(s) 28, 156
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 4 cut(s) 233, 434, 437, 608
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 5 cut(s) 113, 290, 399, 737, 801
TspRI CASTG 2 cut(s) 28, 156
VpaK11BI GGWCC 1 cut(s) 680
XapI RAATTY 3 cut(s) 12, 210, 278
XceI RCATGY 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 419
ZrmI AGTACT 1 cut(s) 590
Zsp2I ATGCAT 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.