FvH4_5g10280

Belongs to the serpin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000163
Physical Location & Seq
Reverse (-)
5877636 .. 5883053
5418 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10280.t5

Sequence Viewer

Length: 3381 bp
ATGAAGAAGATCTATCCGTTAGTATTTTCCTTTTCTTCCTTTACACTTTCTCTCTTCCGCGCTCTGCAACCTTTCAGGTCTCGCGCCGCAGAATCAAAAGAAAGGACGATTGTTCCTAACTCTTACTCTTTTGGTGCTTCATCCTCGCCAGCTTTTGGACAGCAGAGTTCTGCATTTGGTGCATTTTCTACTCCAGTATTTGGTGGATCGTCTAATCAAAGAAGTCCATTTGGTGGAGCATCTGGTACGTCTGTTCTTGGTCAGAAGCCTGCTTTTGGAGCATTTGGGTTGAGTCCTGCTCAAACAAGTCTATTTGGGAGCACAGCTCAACCATCACAACCAGCATTCAGAAGCAATATGTTTGGTAACACCTCTTCACCATTTGGTGGAAGTCAGCCTGCATTTGGAACTAATACTACCCCAGCCTTTGGTTCAACAAACACCACGGCCTTTGGTTCAACAAATACCCCAGCCTTTGGTACCACCACCAACCCTGCTTTTGGTGGCCAAGCCACTCCAGCCTTTGGGGCCACAAGCGCTTCGCCTTTTGGTGGTACAGGTACTCCGGCCTTTGGTGCACCAAGTACTCCCACCTTTGGCTCAACACCAAGTCCTGCATTTGGAAGCACAGGATCTGCATTTGGGACAACAAGTTCCAGCTTGTTTGGAACTGGGGGAGCATTTGGGGCGACTCCAAGCACCCCGTCTTTTGGTCAATCTAGCTCAGCTTTTGGTACTACAACAAGTGCTCCTGCTTTTGGCCAATCAAGCACAGGTTTTGGCTTTTCATCAAGTACTCCTGCATTTGGCCAATCAAGTTCTACCTTTGGTAGCACCCAATTTGGTGCTTCATCTCCTTTTGGAGGGCAGAGTTCTTCATTTGGGGTTCAGTCAACAACACCAACACTTGGAAACAATGCCTTTGGACAGTCAACGTTTGGGGCCCAACAGGGTGGAAGTAGAGCGACTCCTTACACAGGCACATCTGAACCAGATAGTAATGGACTGGGGAAATTGGAGTCAATTTCAGCAATGCCAGCCTATAAAGAAAAAAGTCACGAGGAATTGAGATGGGAGGATTACCAATTAGGGGATAAAGCTGGACCGTCTCCTGCTGGTGGGGGTGGCTTTGGCATGTCAACTGTACAGACAAATCCTTTGAATCCTATTCATGCAACACCGTTTGCTCAAACGTGTTCAAGTCCTTTTAATCATTCAACCTCATCTAGTTTATTTGCTCCAAAAACCTCATCCTTCCCTTCTGCTGGTTTTGGTGTCACTTCATCTTCACCATTTAGTTCGTCTTCACCGTTTAGTTCGTCGTCACCATTTAGTTCTTCTTGGCCGTTTAGTTCATCAACTCAAGGCAATTTGTTTACACCTGCATCATCAGCCCCAGCATTTGGCTCTGCCCCAGCATTTGGCCAAACATCGTCTCCGTTGCCTTTTAGTTCACCGTCGTCCTTCTCATTCTCTACCGCGGCCACATCATCCACTCCCTCATTCAATTTCCCCACTCCTTCAAACCCGCAGACACAGTCTGCTCAAACAGGCACTATCTCATTTCAGCTGAATCCATCAACCTTTCGTTCAAACTTGTTCAATAATACTTCCAGTTTGCAGAGCAGTTCCTTGGGCACTACAAGCAACCAACTGGGTAATAATCCACCAGCTGAAACGGATTCCAGTACTACTATCACCATAAATGAACCATCATTGAGTAATGGTGGATCAAGTCAGCCTGGTTTTGGCTATACTAGTAGCTCAACTTTGGGCACTATCACCATAAACTCAAGTTCTGGATCAAGGATCACAATAACCCCAGCTTCTTCAACTACTATCACCATAACCCCCACTTCAGGCTCCACTACTGTCACCATAGCTCCAAGTTCCGGCACTACCTCAACTTCTACATCTGGACACACTCCATTTGCATCTCCCTTCGTTGTGCCTTCCCAAGCTTCTCAGCCATCTCAGACTAGTACTTCTATCTTCTACACCATCAATAGTTTTGATCAAATTCAGCCAGGGAACAGAGATGGCTTTGGTTCTTCATTCGAAAGATTGCAGAATAACTCCGGACGGTGGCTTGGTAGTGCATGCCCTGCTACAAATCCGTTTAATCTGCTACACAGGCCTGGTCAGCTTCCATCTGGTCATGCTGCTCCTTCACCATCAGTTCAAAATGGAATTTCGGGATCTTTGACAGCTATGAGGGATCTCATAGAATCCGTCCGCAACCAATCTGATGTCGCACTGAGAATCACAAAAGAACTGCTTTTGACTAAAGGCAAGGACAAGAACATGGTCTACTCTCCTCTGTCCATACATGTGGTTCTTAGCCTGATAGCAGCTGGGTCGACAAAGGGTCACCCCCTCCACGATGAGATGCTGAAGTTCCTCAAGTCCAGGTCCATCGAGCAGCTAAATGAGCTTGCCTCGAATCTTGTCCCTCTGGTCTTTGCCGATGGAACCACAAGTGGCGGCCCTAGCTTGGCATTCGCCAATGGTGTTTGGGTTGAAACTTCTCTCCCAGTCAAGGCTTCCTTCAAAGAGGTATTGGACAATGCGTACAAGGCAGTTCTAAAGCAAGTAGATTTTAGGACCAAACCTGAGGAAGCGCGTTGCGAAGTTAACTCATGGGTGGAGAAGGTGACCAGAGGCCTTATCAAAGATCTTCTTCCATATGAAACAGTTACGTGCGCCACAAGGATCATTCTTGCAAATGCTTTGTACTTCAAAGGAGCTTGGGATCAGAAGTTTATTGAATCAAGAACAAATATGTTTGATTTTCACCTTCTCAGTGGGAAGTCGGTTAAGGCACCCTTCATGACCAGTTGGAAGGATCAGTTTATAAGTGTCTTTGACAGTTTCAAAGTCTTAAAGCTTCCTTACAAACAAGGTGAGGATCACAATCGGCGCTTCTCCATGTACATGTTTCTTCCTAATGCAAGTAATGGGCTGCCATCTCTGGTTGAGCGATTCTGTTCAGAGGCTGGGTTTTTGGACCGCTATCTTCCTTGCAGAAAAGTTGAAGTTGACAAATTTTTAATCCCGAAGTTTAAGATCACTTTTGGCATTGAAGTTTCTCAAGTTCTGGAAACTTTAGGACTGAAGTTGCCATATTTGTCAGAGACGGTGGTTGGTGATGAACCATTTGTTAACTTGATAATCCACAAGTCCTTTATTGAAGTTAATGAAGAAGGCACAGAAGCTGCTGCTGCTACTGCTGCCGTTGCTGCGTTTGGCTGCGCTGCTCCTTGTCCTGTGATTGAGAAGATAGACTTTGTGGCAGATCATCCATTCCTTTTTCTGATCCGAGAAGAAGTGACTGGAGCAGTGATGTTCGTTGGGCATGTCCTCGACCCCATTGCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05146 ko05164 map03013 map05146 map05164
Pfam Domains
Protein Families
Serpin

Protein Analysis

1127

Amino Acids

117.57

Weight (kDa)

7.99

Isoelectric Point (pI)

54.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nup98_GLEBS PF21240 318 - 337 4.5e-06 Nup98, Gle2-binding sequence
Serpin PF00079 723 - 1099 6e-82 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64020
fragaria_vesca FvH4_1g24600 FvH4_1g24640 FvH4_2g15000 FvH4_3g03700 FvH4_3g03710 FvH4_3g09790 FvH4_3g09790 FvH4_3g09810 FvH4_3g09811 FvH4_3g23720 FvH4_3g32870 FvH4_3g32890 FvH4_3g37550 FvH4_3g37560 FvH4_5g01890 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g21100 FvH4_5g21110 FvH4_5g21330 FvH4_5g21331 FvH4_5g21520 FvH4_5g21540 FvH4_5g21970 FvH4_6g01280
malus_domestica MD00G1084800.v1.1 MD00G1141300.v1.1 MD02G1102800.v1.1 MD02G1282200.v1.1 MD02G1282300.v1.1 MD03G1207900.v1.1 MD03G1230000.v1.1 MD04G1136400.v1.1 MD04G1136500.v1.1 MD04G1189000.v1.1 MD05G1248700.v1.1 MD05G1248800.v1.1 MD06G1030400.v1.1 MD10G1229200.v1.1 MD10G1229300.v1.1 MD15G1291400.v1.1 MD15G1431300.v1.1
prunus_persica Prupe.5G041800_v2.0.a1 Prupe.5G041900_v2.0.a1 Prupe.5G042000_v2.0.a1 Prupe.5G042100_v2.0.a1 Prupe.5G042200_v2.0.a1
pyrus_communis pycom02g14630 pycom02g24090 pycom04g12410 pycom04g16760 pycom05g22550 pycom10g19280 pycom12g05670 pycom15g25490 pycom15g38110
rosa_chinensis RchiOBHm_Chr2g0122001 RchiOBHm_Chr2g0163171 RchiOBHm_Chr2g0163181 RchiOBHm_Chr3g0455201 RchiOBHm_Chr5g0005751 RchiOBHm_Chr5g0015501 RchiOBHm_Chr5g0015511 RchiOBHm_Chr5g0015521 RchiOBHm_Chr5g0015531 RchiOBHm_Chr5g0060021 RchiOBHm_Chr7g0187971 RchiOBHm_Chr7g0207951 RchiOBHm_Chr7g0208671 RchiOBHm_Chr7g0208691 RchiOBHm_Chr7g0208941 RchiOBHm_Chr7g0208951 RchiOBHm_Chr7g0208961
rosa_laevigata RLG00000003132 RLG00000003177 RLG00000003178 RLG00000003179 RLG00000003180 RLG00000003201 RLG00000003202 RLG00000003270 RLG00000004739 RLG00000004741 RLG00000004744 RLG00000021379 RLG00000025378 RLG00000031323 RLG00000031324 RLG00000032205 RLG00000032206 RLG00000035339
rosa_multiflora Rmu_co8189380.1_g000001 Rmu_co8284591.1_g000001 Rmu_co8360283.1_g000002 Rmu_co8379053.1_g000001 Rmu_sc0000493.1_g000007 Rmu_sc0000532.1_g000030 Rmu_sc0000532.1_g000031 Rmu_sc0000570.1_g000033 Rmu_sc0000905.1_g000012 Rmu_sc0001730.1_g000002 Rmu_sc0002045.1_g000039 Rmu_sc0003064.1_g000001 Rmu_sc0003064.1_g000002 Rmu_sc0003064.1_g000006 Rmu_sc0003880.1_g000012 Rmu_sc0004103.1_g000003 Rmu_sc0005082.1_g000007 Rmu_sc0011272.1_g000002 Rmu_sc0015523.1_g000021 Rmu_sc0015523.1_g000022 Rmu_sc0019960.1_g000007 Rmu_sc0029514.1_g000001 Rmu_ssc0000422.1_g000010
rosa_roxburghii Rroxscaffold_1G00013660 Rroxscaffold_1G00060980 Rroxscaffold_1G00060990 Rroxscaffold_1G00061000 Rroxscaffold_1G00061010 Rroxscaffold_2G00087490 Rroxscaffold_2G00122080 Rroxscaffold_2G00123490 Rroxscaffold_3G00249930 Rroxscaffold_3G00249940 Rroxscaffold_3G00249950 Rroxscaffold_3G00249980 Rroxscaffold_3G00250310 Rroxscaffold_3G00250320 Rroxscaffold_3G00266850 Rroxscaffold_3G00266870 Rroxscaffold_3G00266910 Rroxscaffold_6G00424480
rosa_rugosa Rorug02G0228900 Rorug02G0236000 Rorug02G0501100 Rorug03G0004700 Rorug03G0004700 Rorug03G0004800 Rorug04G0417800 Rorug05G0027800 Rorug05G0027900 Rorug05G0028000 Rorug05G0327700 Rorug05G0384800 Rorug06G0486000 Rorug06G0486100 Rorug06G0486200 Rorug06G0486200 Rorug06G0486300 Rorug06G0486400 Rorug06G0486500 Rorug07G0100400 Rorug07G0105400 Rorug07G0105400 Rorug07G0107300 Rorug07G0107400 Rorug07G0107500 Rorug07G0113300
rosa_samantha Rh7AG091300 Rh7BG092800 Rh7CG091600 Rh7CG257500 Rh7DG093500
rosa_wichuraiana Rw0G001260 Rw0G017680 Rw2G023470 Rw3G005130 Rw5G004560 Rw5G010480 Rw7G020400 Rw7G020410 Rw7G020540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2855
AarI CACCTGC 1 cut(s) 1390
Acc36I ACCTGC 1 cut(s) 1390
Acc65I GGTACC 1 cut(s) 479
AccB1I GGYRCC 2 cut(s) 479, 2821
AccI GTMKAC 2 cut(s) 2310, 2360
AccII CGCG 4 cut(s) 60, 84, 1481, 2623
AccIII TCCGGA 1 cut(s) 2078
AciI CCGC 8 cut(s) 58, 87, 1479, 1481, 1529, 2236, 2484, 3010
AclI AACGTT 1 cut(s) 935
AcoI YGGCCR 6 cut(s) 505, 760, 808, 1343, 1423, 1482
AcsI RAATTY 3 cut(s) 2019, 2190, 3044
AcuI CTGAAG 3 cut(s) 1842, 2414, 3134
AfeI AGCGCT 1 cut(s) 538
AflIII ACRYGT 3 cut(s) 1193, 2329, 2934
AhdI GACNNNNNGTC 1 cut(s) 2367
AhlI ACTAGT 2 cut(s) 1757, 1979
AjnI CCWGG 4 cut(s) 1741, 2026, 2137, 2408
AloI GAACNNNNNNTCC 2 cut(s) 637, 669
Alw21I GWGCWC 3 cut(s) 323, 580, 751
Alw26I GTCTC 4 cut(s) 84, 1113, 1440, 3128
Alw44I GTGCAC 1 cut(s) 576
AlwNI CAGNNNCTG 3 cut(s) 635, 2996, 3215
Aor13HI TCCGGA 1 cut(s) 2078
Aor51HI AGCGCT 1 cut(s) 538
ApaI GGGCCC 1 cut(s) 946
ApaLI GTGCAC 1 cut(s) 576
ApoI RAATTY 3 cut(s) 2019, 2190, 3044
ArsI GACNNNNNNTTYG 2 cut(s) 1141, 1173
Asp718I GGTACC 1 cut(s) 479
AspLEI GCGC 7 cut(s) 62, 86, 539, 2623, 2705, 2922, 3254
AspS9I GGNCC 8 cut(s) 528, 942, 943, 1103, 2412, 2486, 2604, 3007
AsuII TTCGAA 1 cut(s) 2058
AvaII GGWCC 4 cut(s) 1103, 2412, 2604, 3007
AxyI CCTNAGG 1 cut(s) 2613
BaeGI GKGCMC 4 cut(s) 580, 946, 1640, 1778
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BalI TGGCCA 4 cut(s) 507, 762, 810, 1425
BanI GGYRCC 2 cut(s) 479, 2821
BanII GRGCYC 1 cut(s) 946
BarI GAAGNNNNNNTAC 2 cut(s) 358, 390
BauI CACGAG 1 cut(s) 1058
BbsI GAAGAC 1 cut(s) 1296
Bbv12I GWGCWC 3 cut(s) 323, 580, 751
BceAI ACGGC 3 cut(s) 462, 1330, 3218
BcgI CGANNNNNNTGC 3 cut(s) 2340, 2374, 3353
BciT130I CCWGG 4 cut(s) 1743, 2028, 2139, 2410
BclI TGATCA 1 cut(s) 2014
BcoDI GTCTC 4 cut(s) 84, 1113, 1440, 3128
BcuI ACTAGT 2 cut(s) 1757, 1979
BfaI CTAG 5 cut(s) 720, 1227, 1758, 1980, 2490
BfoI RGCGCY 2 cut(s) 540, 2923
BfuAI ACCTGC 1 cut(s) 1390
BglI GCCNNNNNGGC 1 cut(s) 527
BglII AGATCT 2 cut(s) 9, 2674
BlpI GCTNAGC 1 cut(s) 724
BmcAI AGTACT 4 cut(s) 586, 796, 1690, 1984
Bme1390I CCNGG 4 cut(s) 1743, 2028, 2139, 2410
Bme18I GGWCC 4 cut(s) 1103, 2412, 2604, 3007
BmeRI GACNNNNNGTC 1 cut(s) 2367
BmgT120I GGNCC 8 cut(s) 528, 942, 943, 1103, 2412, 2486, 2604, 3007
BmiI GGNNCC 7 cut(s) 481, 529, 943, 944, 1864, 2473, 2823
BmrFI CCNGG 4 cut(s) 1743, 2028, 2139, 2410
BmrI ACTGGG 4 cut(s) 681, 1016, 1664, 2528
BmsI GCATC 4 cut(s) 248, 1394, 1943, 2379
BmuI ACTGGG 4 cut(s) 681, 1016, 1664, 2528
BpiI GAAGAC 1 cut(s) 1296
BplI GAGNNNNNCTC 6 cut(s) 283, 315, 310, 342, 2423, 2455
BpmI CTGGAG 3 cut(s) 177, 501, 3354
Bpu1102I GCTNAGC 1 cut(s) 724
Bpu14I TTCGAA 1 cut(s) 2058
BpuEI CTTGAG 4 cut(s) 1347, 1777, 2387, 3075
BsaAI YACGTR 1 cut(s) 2700
BsaBI GATNNNNATC 1 cut(s) 2913
BsaI GGTCTC 1 cut(s) 84
BsaJI CCNNGG 4 cut(s) 444, 1479, 1632, 2027
BsaWI WCCGGW 1 cut(s) 2078
BsaXI ACNNNNNCTCC 8 cut(s) 547, 577, 733, 763, 1420, 1450, 1867, 1897
Bse1I ACTGG 9 cut(s) 194, 676, 1011, 1614, 1659, 1686, 2534, 2835, 3337
Bse21I CCTNAGG 1 cut(s) 2613
Bse3DI GCAATG 2 cut(s) 1038, 3369
Bse8I GATNNNNATC 1 cut(s) 2913
BseAI TCCGGA 1 cut(s) 2078
BseBI CCWGG 4 cut(s) 1743, 2028, 2139, 2410
BseDI CCNNGG 4 cut(s) 444, 1479, 1632, 2027
BseGI GGATG 4 cut(s) 140, 1250, 1490, 3298
BseJI GATNNNNATC 1 cut(s) 2913
BseMI GCAATG 2 cut(s) 1038, 3369
BseMII CTCAG 6 cut(s) 738, 1979, 1988, 2249, 2604, 2815
BseNI ACTGG 9 cut(s) 194, 676, 1011, 1614, 1659, 1686, 2534, 2835, 3337
BseRI GAGGAG 1 cut(s) 2307
BseSI GKGCMC 4 cut(s) 580, 946, 1640, 1778
BseYI CCCAGC 7 cut(s) 421, 469, 1396, 1414, 1822, 2354, 2996
Bsh1236I CGCG 4 cut(s) 60, 84, 1481, 2623
BshNI GGYRCC 2 cut(s) 479, 2821
BsiHKAI GWGCWC 3 cut(s) 323, 580, 751
BsiSI CCGG 3 cut(s) 566, 1893, 2079
BslFI GGGAC 2 cut(s) 658, 2435
BsmAI GTCTC 4 cut(s) 84, 1113, 1440, 3128
BsmBI CGTCTC 3 cut(s) 1113, 1440, 3128
BsmFI GGGAC 2 cut(s) 658, 2435
BsmI GAATGC 2 cut(s) 344, 2498
Bso31I GGTCTC 1 cut(s) 84
Bsp119I TTCGAA 1 cut(s) 2058
Bsp120I GGGCCC 1 cut(s) 942
Bsp1286I GDGCHC 6 cut(s) 323, 580, 751, 946, 1640, 1778
Bsp13I TCCGGA 1 cut(s) 2078
Bsp1407I TGTACA 2 cut(s) 1144, 2931
Bsp1720I GCTNAGC 1 cut(s) 724
BspACI CCGC 8 cut(s) 58, 87, 1479, 1481, 1529, 2236, 2484, 3010
BspCNI CTCAG 6 cut(s) 737, 1978, 1987, 2250, 2605, 2814
BspEI TCCGGA 1 cut(s) 2078
BspFNI CGCG 4 cut(s) 60, 84, 1481, 2623
BspHI TCATGA 1 cut(s) 2829
BspLI GGNNCC 7 cut(s) 481, 529, 943, 944, 1864, 2473, 2823
BspMI ACCTGC 1 cut(s) 1390
BspT104I TTCGAA 1 cut(s) 2058
BspT107I GGYRCC 2 cut(s) 479, 2821
BspTNI GGTCTC 1 cut(s) 84
BsrDI GCAATG 2 cut(s) 1038, 3369
BsrGI TGTACA 2 cut(s) 1144, 2931
BsrI ACTGG 9 cut(s) 194, 676, 1011, 1614, 1659, 1686, 2534, 2835, 3337
BssECI CCNNGG 4 cut(s) 444, 1479, 1632, 2027
BssSI CACGAG 1 cut(s) 1058
BssT1I CCWWGG 1 cut(s) 1632
Bst2BI CACGAG 1 cut(s) 1058
Bst2UI CCWGG 4 cut(s) 1743, 2028, 2139, 2410
Bst6I CTCTTC 2 cut(s) 59, 379
BstAPI GCANNNNNTGC 2 cut(s) 179, 2105
BstAUI TGTACA 2 cut(s) 1144, 2931
BstBAI YACGTR 1 cut(s) 2700
BstBI TTCGAA 1 cut(s) 2058
BstC8I GCNNGC 6 cut(s) 150, 270, 399, 1038, 2101, 2436
BstDEI CTNAG 7 cut(s) 724, 1965, 1974, 2258, 2339, 2613, 2801
BstDSI CCRYGG 2 cut(s) 444, 1479
BstEII GGTNACC 2 cut(s) 2369, 2653
BstENI CCTNNNNNAGG 2 cut(s) 861, 975
BstF5I GGATG 4 cut(s) 140, 1250, 1490, 3298
BstFNI CGCG 4 cut(s) 60, 84, 1481, 2623
BstH2I RGCGCY 2 cut(s) 540, 2923
BstHHI GCGC 7 cut(s) 62, 86, 539, 2623, 2705, 2922, 3254
BstMAI GTCTC 4 cut(s) 84, 1113, 1440, 3128
BstNI CCWGG 4 cut(s) 1743, 2028, 2139, 2410
BstNSI RCATGY 5 cut(s) 1138, 2103, 2333, 2938, 3359
BstPI GGTNACC 2 cut(s) 2369, 2653
BstSCI CCNGG 4 cut(s) 1741, 2026, 2137, 2408
BstSLI GKGCMC 4 cut(s) 580, 946, 1640, 1778
BstUI CGCG 4 cut(s) 60, 84, 1481, 2623
BstV2I GAAGAC 1 cut(s) 1296
BstX2I RGATCY 5 cut(s) 9, 632, 2198, 2218, 2674
BstXI CCANNNNNNTGG 2 cut(s) 953, 2332
BstYI RGATCY 5 cut(s) 9, 632, 2198, 2218, 2674
Bsu36I CCTNAGG 1 cut(s) 2613
BtgI CCRYGG 2 cut(s) 444, 1479
BtsCI GGATG 4 cut(s) 140, 1250, 1490, 3298
BtsI GCAGTG 1 cut(s) 3345
BtsIMutI CAGTG 3 cut(s) 2255, 2809, 3345
BveI ACCTGC 1 cut(s) 1390
Cac8I GCNNGC 6 cut(s) 150, 270, 399, 1038, 2101, 2436
CaiI CAGNNNCTG 3 cut(s) 635, 2996, 3215
CciI TCATGA 1 cut(s) 2829
CfoI GCGC 7 cut(s) 62, 86, 539, 2623, 2705, 2922, 3254
Cfr13I GGNCC 8 cut(s) 528, 942, 943, 1103, 2412, 2486, 2604, 3007
Cfr42I CCGCGG 1 cut(s) 1482
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
DdeI CTNAG 7 cut(s) 724, 1965, 1974, 2258, 2339, 2613, 2801
DriI GACNNNNNGTC 1 cut(s) 2367
EaeI YGGCCR 6 cut(s) 505, 760, 808, 1343, 1423, 1482
Eam1104I CTCTTC 2 cut(s) 59, 379
Eam1105I GACNNNNNGTC 1 cut(s) 2367
EarI CTCTTC 2 cut(s) 59, 379
Eco130I CCWWGG 1 cut(s) 1632
Eco147I AGGCCT 2 cut(s) 2137, 2664
Eco24I GRGCYC 1 cut(s) 946
Eco31I GGTCTC 1 cut(s) 84
Eco47I GGWCC 4 cut(s) 1103, 2412, 2604, 3007
Eco47III AGCGCT 1 cut(s) 538
Eco57I CTGAAG 3 cut(s) 1842, 2414, 3134
Eco81I CCTNAGG 1 cut(s) 2613
Eco91I GGTNACC 2 cut(s) 2369, 2653
EcoNI CCTNNNNNAGG 2 cut(s) 861, 975
EcoO109I RGGNCCY 1 cut(s) 942
EcoO65I GGTNACC 2 cut(s) 2369, 2653
EcoRII CCWGG 4 cut(s) 1741, 2026, 2137, 2408
EcoT14I CCWWGG 1 cut(s) 1632
EcoT38I GRGCYC 1 cut(s) 946
ErhI CCWWGG 1 cut(s) 1632
Esp3I CGTCTC 3 cut(s) 1113, 1440, 3128
FaqI GGGAC 2 cut(s) 658, 2435
FauI CCCGC 1 cut(s) 1536
FauNDI CATATG 1 cut(s) 2686
FbaI TGATCA 1 cut(s) 2014
FblI GTMKAC 2 cut(s) 2310, 2360
FokI GGATG 4 cut(s) 127, 1237, 1477, 3285
FriOI GRGCYC 1 cut(s) 946
FspBI CTAG 5 cut(s) 720, 1227, 1758, 1980, 2490
GlaI GCGC 7 cut(s) 61, 85, 538, 2622, 2704, 2921, 3253
GsaI CCCAGC 7 cut(s) 425, 473, 1400, 1418, 1826, 2358, 3000
GsuI CTGGAG 3 cut(s) 177, 501, 3354
HaeII RGCGCY 2 cut(s) 540, 2923
HapII CCGG 3 cut(s) 566, 1893, 2079
HhaI GCGC 7 cut(s) 62, 86, 539, 2623, 2705, 2922, 3254
Hin6I GCGC 7 cut(s) 60, 84, 537, 2621, 2703, 2920, 3252
HinP1I GCGC 7 cut(s) 60, 84, 537, 2621, 2703, 2920, 3252
HincII GTYRAC 7 cut(s) 894, 933, 1140, 2361, 2635, 3040, 3163
HindII GTYRAC 7 cut(s) 894, 933, 1140, 2361, 2635, 3040, 3163
HindIII AAGCTT 2 cut(s) 1959, 2885
HpaI GTTAAC 2 cut(s) 2635, 3163
HpaII CCGG 3 cut(s) 566, 1893, 2079
Hpy188III TCNNGA 9 cut(s) 1058, 1800, 1917, 2079, 2196, 2772, 2830, 3055, 3098
Hpy99I CGWCG 2 cut(s) 1324, 1462
HpyCH4IV ACGT 4 cut(s) 248, 935, 1193, 2699
HpyF3I CTNAG 7 cut(s) 724, 1965, 1974, 2258, 2339, 2613, 2801
HpySE526I ACGT 4 cut(s) 248, 935, 1193, 2699
HspAI GCGC 7 cut(s) 60, 84, 537, 2621, 2703, 2920, 3252
Kpn2I TCCGGA 1 cut(s) 2078
KpnI GGTACC 1 cut(s) 483
Ksp22I TGATCA 1 cut(s) 2014
KspAI GTTAAC 2 cut(s) 2635, 3163
KspI CCGCGG 1 cut(s) 1482
LweI GCATC 4 cut(s) 248, 1394, 1943, 2379
MaeI CTAG 5 cut(s) 720, 1227, 1758, 1980, 2490
MaeII ACGT 4 cut(s) 248, 935, 1193, 2699
MaeIII GTNAC 9 cut(s) 365, 1055, 1276, 1323, 1873, 2369, 2653, 2695, 3328
MflI RGATCY 5 cut(s) 9, 632, 2198, 2218, 2674
MhlI GDGCHC 6 cut(s) 323, 580, 751, 946, 1640, 1778
MlsI TGGCCA 4 cut(s) 507, 762, 810, 1425
MluNI TGGCCA 4 cut(s) 507, 762, 810, 1425
MlyI GAGTC 4 cut(s) 301, 685, 961, 1028
MmeI TCCRAC 1 cut(s) 2819
Mox20I TGGCCA 4 cut(s) 507, 762, 810, 1425
MroI TCCGGA 1 cut(s) 2078
MscI TGGCCA 4 cut(s) 507, 762, 810, 1425
MseI TTAA 9 cut(s) 1209, 2121, 2634, 2817, 2882, 3050, 3063, 3162, 3195
MslI CAYNNNNRTG 2 cut(s) 2330, 2933
Msp20I TGGCCA 4 cut(s) 507, 762, 810, 1425
MspA1I CMGCKG 4 cut(s) 1481, 1570, 1673, 2354
MspI CCGG 3 cut(s) 566, 1893, 2079
MspR9I CCNGG 4 cut(s) 1743, 2028, 2139, 2410
Mva1269I GAATGC 2 cut(s) 344, 2498
MvaI CCWGG 4 cut(s) 1743, 2028, 2139, 2410
MvnI CGCG 4 cut(s) 60, 84, 1481, 2623
NdeI CATATG 1 cut(s) 2686
NlaIV GGNNCC 7 cut(s) 481, 529, 943, 944, 1864, 2473, 2823
NmuCI GTSAC 7 cut(s) 1055, 1276, 1323, 1873, 2369, 2653, 3328
NspI RCATGY 5 cut(s) 1138, 2103, 2333, 2938, 3359
NspV TTCGAA 1 cut(s) 2058
PaeI GCATGC 1 cut(s) 2103
PagI TCATGA 1 cut(s) 2829
PaqCI CACCTGC 1 cut(s) 1390
PceI AGGCCT 2 cut(s) 2137, 2664
PciI ACATGT 2 cut(s) 2329, 2934
PcsI WCGNNNNNNNCGW 1 cut(s) 1307
PctI GAATGC 2 cut(s) 344, 2498
PfeI GAWTC 9 cut(s) 92, 1162, 1573, 1682, 2228, 2262, 2443, 2768, 2982
PflFI GACNNNGTC 1 cut(s) 1537
PleI GAGTC 4 cut(s) 300, 685, 961, 1027
PpsI GAGTC 4 cut(s) 300, 685, 961, 1027
Ppu21I YACGTR 1 cut(s) 2700
PscI ACATGT 2 cut(s) 2329, 2934
PsiI TTATAA 1 cut(s) 2855
Psp1406I AACGTT 1 cut(s) 935
Psp6I CCWGG 4 cut(s) 1741, 2026, 2137, 2408
PspEI GGTNACC 2 cut(s) 2369, 2653
PspFI CCCAGC 7 cut(s) 421, 469, 1396, 1414, 1822, 2354, 2996
PspGI CCWGG 4 cut(s) 1741, 2026, 2137, 2408
PspN4I GGNNCC 7 cut(s) 481, 529, 943, 944, 1864, 2473, 2823
PspOMI GGGCCC 1 cut(s) 942
PspPI GGNCC 8 cut(s) 528, 942, 943, 1103, 2412, 2486, 2604, 3007
PsrI GAACNNNNNNTAC 4 cut(s) 400, 432, 2294, 2326
PstNI CAGNNNCTG 3 cut(s) 635, 2996, 3215
PsuI RGATCY 5 cut(s) 9, 632, 2198, 2218, 2674
PsyI GACNNNGTC 1 cut(s) 1537
PvuII CAGCTG 3 cut(s) 1570, 1673, 2354
RseI CAYNNNNRTG 2 cut(s) 2330, 2933
SacII CCGCGG 1 cut(s) 1482
SalI GTCGAC 1 cut(s) 2359
SaqAI TTAA 9 cut(s) 1209, 2121, 2634, 2817, 2882, 3050, 3063, 3162, 3195
Sau96I GGNCC 8 cut(s) 528, 942, 943, 1103, 2412, 2486, 2604, 3007
ScaI AGTACT 4 cut(s) 586, 796, 1690, 1984
SchI GAGTC 4 cut(s) 301, 685, 961, 1028
ScrFI CCNGG 4 cut(s) 1743, 2028, 2139, 2410
SduI GDGCHC 6 cut(s) 323, 580, 751, 946, 1640, 1778
SfaNI GCATC 4 cut(s) 248, 1394, 1943, 2379
Sfr303I CCGCGG 1 cut(s) 1482
SfuI TTCGAA 1 cut(s) 2058
SgrBI CCGCGG 1 cut(s) 1482
SinI GGWCC 4 cut(s) 1103, 2412, 2604, 3007
SmiMI CAYNNNNRTG 2 cut(s) 2330, 2933
SmlI CTYRAG 4 cut(s) 1362, 1792, 2402, 3090
SmoI CTYRAG 4 cut(s) 1362, 1792, 2402, 3090
SpeI ACTAGT 2 cut(s) 1757, 1979
SphI GCATGC 1 cut(s) 2103
SseBI AGGCCT 2 cut(s) 2137, 2664
SsiI CCGC 8 cut(s) 58, 87, 1479, 1481, 1529, 2236, 2484, 3010
SspMI CTAG 5 cut(s) 720, 1227, 1758, 1980, 2490
StuI AGGCCT 2 cut(s) 2137, 2664
StyD4I CCNGG 4 cut(s) 1741, 2026, 2137, 2408
StyI CCWWGG 1 cut(s) 1632
TaiI ACGT 4 cut(s) 251, 938, 1196, 2702
TaqI TCGA 5 cut(s) 2058, 2360, 2418, 2441, 3363
TatI WGTACW 7 cut(s) 584, 794, 1144, 1688, 1982, 2733, 2931
TauI GCSGC 3 cut(s) 89, 1484, 2487
TfiI GAWTC 9 cut(s) 92, 1162, 1573, 1682, 2228, 2262, 2443, 2768, 2982
Tru1I TTAA 9 cut(s) 1209, 2121, 2634, 2817, 2882, 3050, 3063, 3162, 3195
Tru9I TTAA 9 cut(s) 1209, 2121, 2634, 2817, 2882, 3050, 3063, 3162, 3195
TscAI CASTG 3 cut(s) 2262, 2809, 3345
TseFI GTSAC 7 cut(s) 1055, 1276, 1323, 1873, 2369, 2653, 3328
Tsp45I GTSAC 7 cut(s) 1055, 1276, 1323, 1873, 2369, 2653, 3328
TspGWI ACGGA 5 cut(s) 6, 1428, 1694, 2106, 2221
TspRI CASTG 3 cut(s) 2262, 2809, 3345
Tth111I GACNNNGTC 1 cut(s) 1537
VneI GTGCAC 1 cut(s) 576
VpaK11BI GGWCC 4 cut(s) 1103, 2412, 2604, 3007
XagI CCTNNNNNAGG 2 cut(s) 861, 975
XapI RAATTY 3 cut(s) 2019, 2190, 3044
XceI RCATGY 5 cut(s) 1138, 2103, 2333, 2938, 3359
XmiI GTMKAC 2 cut(s) 2310, 2360
XspI CTAG 5 cut(s) 720, 1227, 1758, 1980, 2490
ZrmI AGTACT 4 cut(s) 586, 796, 1690, 1984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.