MD05G1248800.v1.1

Belongs to the serpin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000163
Physical Location & Seq
Reverse (-)
38051322 .. 38053376
2055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1248800.v1.1.491

Sequence Viewer

Length: 1758 bp
ATGTTCGGAGATCAATCTTCGTCGTCCCCATACGTGACGCCTTCGTCAACCGCAGCCTTCGGGCCGCCGTCTTCAACCCCAGCCATGGGAACGCCATCTGGGAACTCAGCCTTCAGGCCGCCGTCGTCGACCCCAGCCTTCGGACCGCCGCCGTCGACCCCAGCCTTCGGGCCGCCGTCGTCAACCTCAGGCTTCGGTCCGCCATCTTGGAACCCAGGCTTCGGGACGTCGTCTTGGAACCCAGCCTTGGGGCCGCCGTCTTGGAACCCAGACTTCGGACCGCCGTCTAGGAACCCAGCCTTCAGGCCGCCTTCGTCGACCCCAGACTTGGGGACGCCGTCTTGGAACCCCTCCTTCGTCACTCCTTGGACCCCGGCCTTTGTAACTCCGTCGACGTTTCAGCAGCAGACGCCGTTCTTTAACTTCCAGCAGCCTCATTCTCCTGGCGCCTTTGAATTTGAATTTGAGAGTTATATGCCGTTGTTTGAAGAGCCGACACCCTTCCCTGACTTTGATTTGATGACCGAGATTGATCATGTTTCTCTCCCCTTCTCTCTCCCCTGTCGCAATATCCCCTTCTCTCTCCCCAATCGCGATATTAAAGTAACTTTCCCTTTTGTTATTGATTGTTCTCCATCGACCAAAGAATCCATCAGCAACCTAACCGATGTCTCTCTCAAAATTACTAAGCATCTACTCACCGCTGAAGGCAAAGACAAAAACATGGTGTATTCGCCGCTGTCCATTCAGATCGTCCTGTGGCTGTTAACGGCCGGGTCAAAGGGTCCCACAAAGGGGCAGTTGCTCTCTTTCCTTAAGTCCGAGTCCGTCGACGAACTCAACTCCCTCGCGAGCGGTCCCTGCCTATCATTTGCCAATGGCCTTTGGGTTAAGGAGTCTCTCTCCATCAAACCTTCTTTCAAAGAGGTTGTGGACATTTCTTACAAGGCAGATATAAACCATGTTAGTTTCCAAAACCCTGAAAAAGTGAGACTTGAAGTGAATTTATGGGCAGAAAAGGCAACGAAAGGCCTTATCACTGAGGCACTTCCTCCTGGGTTAGTTGACAAGAAAACCGTTCTTATATTTTCAAATGCCTTATACTTCAAAGGCCTTTGGAGCAACAAGTTCAATGCATCCAATACAAAAGAGGATTTTTTCCGCCTTCTCAACGGTCATTATGTTAAGGCACAGTTCATGAGAAGCTACGAGGCCCAGTTTGTAGAAGCCTTTGACGGCTTCAAAGTTGCAAAGCTCCCTTACAAACAAGGCGAAGACAAGGAGAGGCAGTTTTCTATGTACTTTTTACTTCCACATGCAAGAAATGGGCTGCCAGCTTTGGTTGAAAGAGTTTGTTCCGAGCCTGGTTTCTTAGATCGTCATCGCCCAGCAAGACAAGTCCCAGTTGGTGCCTTCAAAATCCCCAGGTTTAAGGTTACCTCTGGCTTTAAAGCTTCCGATATTCTCCAGGATTTAGGACTGGTGTTGCCATTCACTGATGGTTCGACAGAGATGGTGGAGTCAGATATAATTAAGGGAAAGATATTTCATATATCCTTCATCGAAGTTGAAGAAGAAGGCACAAAAGCTGCAGCTGTTTCTACTTTTGTCGGTTATTCAGCTGCATCGGCTAGACCGATTGTACCGATTAAAAAGATAGACTTCGTGGCTGATCACCCATTCCTCTTTTTTATTAGAGAGGAAAGGACTGGAACGGTTATGTTTATTGGTCAAGTGCTCAATCCTCTTAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05146 ko05164 map03013 map05146 map05164
Pfam Domains
Protein Families
Serpin

Protein Analysis

586

Amino Acids

64.42

Weight (kDa)

6.36

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 220 - 582 3.1e-78 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64020
fragaria_vesca FvH4_1g24600 FvH4_1g24640 FvH4_2g15000 FvH4_3g03700 FvH4_3g03710 FvH4_3g09790 FvH4_3g09790 FvH4_3g09810 FvH4_3g09811 FvH4_3g23720 FvH4_3g32870 FvH4_3g32890 FvH4_3g37550 FvH4_3g37560 FvH4_5g01890 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g21100 FvH4_5g21110 FvH4_5g21330 FvH4_5g21331 FvH4_5g21520 FvH4_5g21540 FvH4_5g21970 FvH4_6g01280
malus_domestica MD00G1084800.v1.1 MD00G1141300.v1.1 MD02G1102800.v1.1 MD02G1282200.v1.1 MD02G1282300.v1.1 MD03G1207900.v1.1 MD03G1230000.v1.1 MD04G1136400.v1.1 MD04G1136500.v1.1 MD04G1189000.v1.1 MD05G1248700.v1.1 MD05G1248800.v1.1 MD06G1030400.v1.1 MD10G1229200.v1.1 MD10G1229300.v1.1 MD15G1291400.v1.1 MD15G1431300.v1.1
prunus_persica Prupe.5G041800_v2.0.a1 Prupe.5G041900_v2.0.a1 Prupe.5G042000_v2.0.a1 Prupe.5G042100_v2.0.a1 Prupe.5G042200_v2.0.a1
pyrus_communis pycom02g14630 pycom02g24090 pycom04g12410 pycom04g16760 pycom05g22550 pycom10g19280 pycom12g05670 pycom15g25490 pycom15g38110
rosa_chinensis RchiOBHm_Chr2g0122001 RchiOBHm_Chr2g0163171 RchiOBHm_Chr2g0163181 RchiOBHm_Chr3g0455201 RchiOBHm_Chr5g0005751 RchiOBHm_Chr5g0015501 RchiOBHm_Chr5g0015511 RchiOBHm_Chr5g0015521 RchiOBHm_Chr5g0015531 RchiOBHm_Chr5g0060021 RchiOBHm_Chr7g0187971 RchiOBHm_Chr7g0207951 RchiOBHm_Chr7g0208671 RchiOBHm_Chr7g0208691 RchiOBHm_Chr7g0208941 RchiOBHm_Chr7g0208951 RchiOBHm_Chr7g0208961
rosa_laevigata RLG00000003132 RLG00000003177 RLG00000003178 RLG00000003179 RLG00000003180 RLG00000003201 RLG00000003202 RLG00000003270 RLG00000004739 RLG00000004741 RLG00000004744 RLG00000021379 RLG00000025378 RLG00000031323 RLG00000031324 RLG00000032205 RLG00000032206 RLG00000035339
rosa_multiflora Rmu_co8189380.1_g000001 Rmu_co8284591.1_g000001 Rmu_co8360283.1_g000002 Rmu_co8379053.1_g000001 Rmu_sc0000493.1_g000007 Rmu_sc0000532.1_g000030 Rmu_sc0000532.1_g000031 Rmu_sc0000570.1_g000033 Rmu_sc0000905.1_g000012 Rmu_sc0001730.1_g000002 Rmu_sc0002045.1_g000039 Rmu_sc0003064.1_g000001 Rmu_sc0003064.1_g000002 Rmu_sc0003064.1_g000006 Rmu_sc0003880.1_g000012 Rmu_sc0004103.1_g000003 Rmu_sc0005082.1_g000007 Rmu_sc0011272.1_g000002 Rmu_sc0015523.1_g000021 Rmu_sc0015523.1_g000022 Rmu_sc0019960.1_g000007 Rmu_sc0029514.1_g000001 Rmu_ssc0000422.1_g000010
rosa_roxburghii Rroxscaffold_1G00013660 Rroxscaffold_1G00060980 Rroxscaffold_1G00060990 Rroxscaffold_1G00061000 Rroxscaffold_1G00061010 Rroxscaffold_2G00087490 Rroxscaffold_2G00122080 Rroxscaffold_2G00123490 Rroxscaffold_3G00249930 Rroxscaffold_3G00249940 Rroxscaffold_3G00249950 Rroxscaffold_3G00249980 Rroxscaffold_3G00250310 Rroxscaffold_3G00250320 Rroxscaffold_3G00266850 Rroxscaffold_3G00266870 Rroxscaffold_3G00266910 Rroxscaffold_6G00424480
rosa_rugosa Rorug02G0228900 Rorug02G0236000 Rorug02G0501100 Rorug03G0004700 Rorug03G0004700 Rorug03G0004800 Rorug04G0417800 Rorug05G0027800 Rorug05G0027900 Rorug05G0028000 Rorug05G0327700 Rorug05G0384800 Rorug06G0486000 Rorug06G0486100 Rorug06G0486200 Rorug06G0486200 Rorug06G0486300 Rorug06G0486400 Rorug06G0486500 Rorug07G0100400 Rorug07G0105400 Rorug07G0105400 Rorug07G0107300 Rorug07G0107400 Rorug07G0107500 Rorug07G0113300
rosa_samantha Rh7AG091300 Rh7BG092800 Rh7CG091600 Rh7CG257500 Rh7DG093500
rosa_wichuraiana Rw0G001260 Rw0G017680 Rw2G023470 Rw3G005130 Rw5G004560 Rw5G010480 Rw7G020400 Rw7G020410 Rw7G020540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 43
AatII GACGTC 1 cut(s) 230
AccB1I GGYRCC 2 cut(s) 446, 1409
AccBSI CCGCTC 1 cut(s) 855
AccI GTMKAC 5 cut(s) 128, 155, 317, 392, 831
AccII CGCG 2 cut(s) 594, 851
AcoI YGGCCR 1 cut(s) 771
AcsI RAATTY 3 cut(s) 455, 461, 1003
AcuI CTGAAG 3 cut(s) 97, 286, 726
AcyI GRCGYC 5 cut(s) 38, 227, 335, 410, 447
AfaI GTAC 2 cut(s) 1301, 1644
AflII CTTAAG 1 cut(s) 815
AjnI CCWGG 6 cut(s) 214, 442, 1054, 1363, 1424, 1467
AjuI GAANNNNNNNTTGG 2 cut(s) 230, 262
AluBI AGCT 7 cut(s) 1206, 1255, 1337, 1454, 1589, 1595, 1622
AluI AGCT 7 cut(s) 1206, 1255, 1337, 1454, 1589, 1595, 1622
Alw21I GWGCWC 1 cut(s) 1740
Alw26I GTCTC 3 cut(s) 676, 903, 985
ApeKI GCWGC 7 cut(s) 53, 403, 430, 1330, 1589, 1592, 1622
ApoI RAATTY 3 cut(s) 455, 461, 1003
AspLEI GCGC 1 cut(s) 449
AsuC2I CCSGG 2 cut(s) 374, 775
AsuHPI GGTGA 2 cut(s) 691, 1667
AvaII GGWCC 6 cut(s) 143, 197, 278, 369, 785, 857
AxyI CCTNAGG 1 cut(s) 187
BanI GGYRCC 2 cut(s) 446, 1409
BbsI GAAGAC 2 cut(s) 63, 1281
Bbv12I GWGCWC 1 cut(s) 1740
BbvI GCAGC 7 cut(s) 65, 415, 442, 1317, 1576, 1604, 1609
BccI CCATC 7 cut(s) 103, 211, 643, 659, 914, 1493, 1507
BciT130I CCWGG 6 cut(s) 216, 444, 1056, 1365, 1426, 1469
BclI TGATCA 2 cut(s) 532, 1672
BcnI CCSGG 2 cut(s) 374, 775
BcoDI GTCTC 3 cut(s) 676, 903, 985
BfaI CTAG 2 cut(s) 288, 1632
BfmI CTRYAG 1 cut(s) 1590
BfoI RGCGCY 1 cut(s) 450
BfrI CTTAAG 1 cut(s) 815
Bme1390I CCNGG 8 cut(s) 216, 374, 444, 775, 1056, 1365, 1426, 1469
Bme18I GGWCC 6 cut(s) 143, 197, 278, 369, 785, 857
BmrFI CCNGG 8 cut(s) 216, 374, 444, 775, 1056, 1365, 1426, 1469
BmrI ACTGGG 2 cut(s) 1210, 1397
BmsI GCATC 3 cut(s) 700, 1145, 1634
BmuI ACTGGG 2 cut(s) 1210, 1397
BoxI GACNNNNGTC 1 cut(s) 283
BpiI GAAGAC 2 cut(s) 63, 1281
BplI GAGNNNNNCTC 2 cut(s) 887, 919
BpmI CTGGAG 1 cut(s) 1451
BpuMI CCSGG 2 cut(s) 374, 775
BsaAI YACGTR 1 cut(s) 34
BsaBI GATNNNNATC 1 cut(s) 1380
BsaHI GRCGYC 5 cut(s) 38, 227, 335, 410, 447
BsaJI CCNNGG 7 cut(s) 84, 214, 246, 365, 372, 1055, 1424
BsaXI ACNNNNNCTCC 2 cut(s) 1274, 1304
Bse1I ACTGG 4 cut(s) 1216, 1403, 1485, 1714
Bse21I CCTNAGG 1 cut(s) 187
Bse8I GATNNNNATC 1 cut(s) 1380
BseBI CCWGG 6 cut(s) 216, 444, 1056, 1365, 1426, 1469
BseDI CCNNGG 7 cut(s) 84, 214, 246, 365, 372, 1055, 1424
BseGI GGATG 1 cut(s) 1136
BseJI GATNNNNATC 1 cut(s) 1380
BseMII CTCAG 3 cut(s) 120, 201, 1032
BseNI ACTGG 4 cut(s) 1216, 1403, 1485, 1714
BseX3I CGGCCG 1 cut(s) 771
BseXI GCAGC 7 cut(s) 65, 415, 442, 1317, 1576, 1604, 1609
BseYI CCCAGC 6 cut(s) 79, 133, 160, 241, 295, 1387
Bsh1236I CGCG 2 cut(s) 594, 851
Bsh1285I CGRYCG 1 cut(s) 774
BshNI GGYRCC 2 cut(s) 446, 1409
BsiEI CGRYCG 1 cut(s) 774
BsiHKAI GWGCWC 1 cut(s) 1740
BsiSI CCGG 2 cut(s) 374, 774
BslFI GGGAC 6 cut(s) 10, 238, 346, 771, 843, 1385
BsmAI GTCTC 3 cut(s) 676, 903, 985
BsmFI GGGAC 6 cut(s) 10, 238, 346, 771, 843, 1385
Bsp1286I GDGCHC 1 cut(s) 1740
Bsp143I GATC 5 cut(s) 10, 532, 750, 1375, 1672
Bsp19I CCATGG 1 cut(s) 84
Bsp68I TCGCGA 2 cut(s) 594, 851
BspCNI CTCAG 3 cut(s) 119, 200, 1033
BspFNI CGCG 2 cut(s) 594, 851
BspHI TCATGA 1 cut(s) 1197
BspMAI CTGCAG 1 cut(s) 1594
BspQI GCTCTTC 1 cut(s) 483
BspT107I GGYRCC 2 cut(s) 446, 1409
BspTI CTTAAG 1 cut(s) 815
BsrBI CCGCTC 1 cut(s) 855
BsrI ACTGG 4 cut(s) 1216, 1403, 1485, 1714
BssECI CCNNGG 7 cut(s) 84, 214, 246, 365, 372, 1055, 1424
BssMI GATC 5 cut(s) 10, 532, 750, 1375, 1672
BssNI GRCGYC 5 cut(s) 38, 227, 335, 410, 447
BssT1I CCWWGG 3 cut(s) 84, 246, 365
Bst2UI CCWGG 6 cut(s) 216, 444, 1056, 1365, 1426, 1469
Bst4CI ACNGT 4 cut(s) 1078, 1175, 1194, 1717
Bst6I CTCTTC 1 cut(s) 483
BstACI GRCGYC 5 cut(s) 38, 227, 335, 410, 447
BstAFI CTTAAG 1 cut(s) 815
BstBAI YACGTR 1 cut(s) 34
BstC8I GCNNGC 2 cut(s) 853, 1335
BstDEI CTNAG 5 cut(s) 106, 187, 687, 1041, 1372
BstDSI CCRYGG 1 cut(s) 84
BstEII GGTNACC 1 cut(s) 1435
BstF5I GGATG 1 cut(s) 1136
BstFNI CGCG 2 cut(s) 594, 851
BstH2I RGCGCY 1 cut(s) 450
BstHHI GCGC 1 cut(s) 449
BstKTI GATC 5 cut(s) 13, 535, 753, 1378, 1675
BstMAI GTCTC 3 cut(s) 676, 903, 985
BstMBI GATC 5 cut(s) 10, 532, 750, 1375, 1672
BstMCI CGRYCG 1 cut(s) 774
BstMWI GCNNNNNNNGC 5 cut(s) 409, 861, 1019, 1119, 1628
BstNI CCWGG 6 cut(s) 216, 444, 1056, 1365, 1426, 1469
BstNSI RCATGY 1 cut(s) 1319
BstPAI GACNNNNGTC 1 cut(s) 283
BstPI GGTNACC 1 cut(s) 1435
BstSCI CCNGG 8 cut(s) 214, 372, 442, 773, 1054, 1363, 1424, 1467
BstSFI CTRYAG 1 cut(s) 1590
BstUI CGCG 2 cut(s) 594, 851
BstV1I GCAGC 7 cut(s) 65, 415, 442, 1317, 1576, 1604, 1609
BstV2I GAAGAC 2 cut(s) 63, 1281
BstZI CGGCCG 1 cut(s) 771
Bsu36I CCTNAGG 1 cut(s) 187
BtgI CCRYGG 1 cut(s) 84
BtgZI GCGATG 1 cut(s) 1367
BtsCI GGATG 1 cut(s) 1136
BtsIMutI CAGTG 2 cut(s) 1038, 1494
BtuMI TCGCGA 2 cut(s) 594, 851
Cac8I GCNNGC 2 cut(s) 853, 1335
CciI TCATGA 1 cut(s) 1197
CfoI GCGC 1 cut(s) 449
CpoI CGGWCCG 3 cut(s) 143, 197, 278
CseI GACGC 3 cut(s) 46, 343, 418
Csp6I GTAC 2 cut(s) 1300, 1643
CspI CGGWCCG 3 cut(s) 143, 197, 278
CviAII CATG 6 cut(s) 85, 536, 724, 962, 1198, 1316
CviQI GTAC 2 cut(s) 1300, 1643
DdeI CTNAG 5 cut(s) 106, 187, 687, 1041, 1372
DinI GGCGCC 1 cut(s) 448
DpnI GATC 5 cut(s) 12, 534, 752, 1377, 1674
DpnII GATC 5 cut(s) 10, 532, 750, 1375, 1672
DraI TTTAAA 1 cut(s) 1450
DrdI GACNNNNNNGTC 1 cut(s) 43
DseDI GACNNNNNNGTC 1 cut(s) 43
EaeI YGGCCR 1 cut(s) 771
EagI CGGCCG 1 cut(s) 771
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
EciI GGCGGA 2 cut(s) 189, 1151
EclXI CGGCCG 1 cut(s) 771
Eco130I CCWWGG 3 cut(s) 84, 246, 365
Eco147I AGGCCT 2 cut(s) 1032, 1113
Eco47I GGWCC 6 cut(s) 143, 197, 278, 369, 785, 857
Eco52I CGGCCG 1 cut(s) 771
Eco57I CTGAAG 3 cut(s) 97, 286, 726
Eco81I CCTNAGG 1 cut(s) 187
Eco91I GGTNACC 1 cut(s) 1435
EcoO109I RGGNCCY 1 cut(s) 785
EcoO65I GGTNACC 1 cut(s) 1435
EcoRII CCWGG 6 cut(s) 214, 442, 1054, 1363, 1424, 1467
EcoT14I CCWWGG 3 cut(s) 84, 246, 365
EcoT22I ATGCAT 1 cut(s) 1138
EgeI GGCGCC 1 cut(s) 448
EheI GGCGCC 1 cut(s) 448
ErhI CCWWGG 3 cut(s) 84, 246, 365
FaeI CATG 6 cut(s) 88, 539, 727, 965, 1201, 1319
FalI AAGNNNNNCTT 4 cut(s) 978, 1010, 1646, 1678
FaqI GGGAC 6 cut(s) 10, 238, 346, 771, 843, 1385
FatI CATG 6 cut(s) 84, 535, 723, 961, 1197, 1315
FbaI TGATCA 2 cut(s) 532, 1672
FblI GTMKAC 5 cut(s) 128, 155, 317, 392, 831
FokI GGATG 1 cut(s) 1123
FspBI CTAG 2 cut(s) 288, 1632
GlaI GCGC 1 cut(s) 448
GsaI CCCAGC 6 cut(s) 83, 137, 164, 245, 299, 1391
GsuI CTGGAG 1 cut(s) 1451
HaeII RGCGCY 1 cut(s) 450
HapII CCGG 2 cut(s) 374, 774
HgaI GACGC 3 cut(s) 46, 343, 418
HhaI GCGC 1 cut(s) 449
Hin1I GRCGYC 5 cut(s) 38, 227, 335, 410, 447
Hin1II CATG 6 cut(s) 88, 539, 727, 965, 1201, 1319
Hin6I GCGC 1 cut(s) 447
HinP1I GCGC 1 cut(s) 447
HincII GTYRAC 9 cut(s) 48, 129, 156, 183, 318, 393, 768, 832, 1066
HindII GTYRAC 9 cut(s) 48, 129, 156, 183, 318, 393, 768, 832, 1066
HindIII AAGCTT 1 cut(s) 1452
HinfI GANTC 4 cut(s) 647, 824, 896, 1520
HpaI GTTAAC 1 cut(s) 768
HpaII CCGG 2 cut(s) 374, 774
HphI GGTGA 2 cut(s) 691, 1667
Hpy188I TCNGA 8 cut(s) 8, 143, 278, 750, 823, 1360, 1459, 1525
Hpy188III TCNNGA 4 cut(s) 223, 593, 850, 1198
HpyCH4III ACNGT 4 cut(s) 1078, 1175, 1194, 1717
HpyCH4IV ACGT 3 cut(s) 33, 227, 395
HpyCH4V TGCA 5 cut(s) 1136, 1250, 1319, 1592, 1625
HpyF10VI GCNNNNNNNGC 5 cut(s) 409, 861, 1019, 1119, 1628
HpyF3I CTNAG 5 cut(s) 106, 187, 687, 1041, 1372
HpySE526I ACGT 3 cut(s) 33, 227, 395
Hsp92I GRCGYC 5 cut(s) 38, 227, 335, 410, 447
Hsp92II CATG 6 cut(s) 88, 539, 727, 965, 1201, 1319
HspAI GCGC 1 cut(s) 447
KasI GGCGCC 1 cut(s) 446
KflI GGGWCCC 1 cut(s) 785
Ksp22I TGATCA 2 cut(s) 532, 1672
KspAI GTTAAC 1 cut(s) 768
Kzo9I GATC 5 cut(s) 10, 532, 750, 1375, 1672
LguI GCTCTTC 1 cut(s) 483
LmnI GCTCC 2 cut(s) 1119, 1260
Lsp1109I GCAGC 7 cut(s) 65, 415, 442, 1317, 1576, 1604, 1609
LweI GCATC 3 cut(s) 700, 1145, 1634
MaeI CTAG 2 cut(s) 288, 1632
MaeII ACGT 3 cut(s) 33, 227, 395
MaeIII GTNAC 5 cut(s) 34, 358, 382, 604, 1435
MalI GATC 5 cut(s) 12, 534, 752, 1377, 1674
MbiI CCGCTC 1 cut(s) 855
MboI GATC 5 cut(s) 10, 532, 750, 1375, 1672
MboII GAAGA 6 cut(s) 9, 63, 500, 1286, 1583, 1586
MhlI GDGCHC 1 cut(s) 1740
MluCI AATT 5 cut(s) 455, 461, 681, 1003, 1530
Mly113I GGCGCC 1 cut(s) 447
MlyI GAGTC 3 cut(s) 833, 905, 1529
Mph1103I ATGCAT 1 cut(s) 1138
MspA1I CMGCKG 4 cut(s) 704, 739, 1595, 1622
MspCI CTTAAG 1 cut(s) 815
MspI CCGG 2 cut(s) 374, 774
MspR9I CCNGG 8 cut(s) 216, 374, 444, 775, 1056, 1365, 1426, 1469
MvaI CCWGG 6 cut(s) 216, 444, 1056, 1365, 1426, 1469
MvnI CGCG 2 cut(s) 594, 851
MwoI GCNNNNNNNGC 5 cut(s) 409, 861, 1019, 1119, 1628
NarI GGCGCC 1 cut(s) 447
NciI CCSGG 2 cut(s) 374, 775
NcoI CCATGG 1 cut(s) 84
NdeII GATC 5 cut(s) 10, 532, 750, 1375, 1672
NlaIII CATG 6 cut(s) 88, 539, 727, 965, 1201, 1319
NmuCI GTSAC 2 cut(s) 34, 358
NruI TCGCGA 2 cut(s) 594, 851
NsiI ATGCAT 1 cut(s) 1138
NspI RCATGY 1 cut(s) 1319
PagI TCATGA 1 cut(s) 1197
PceI AGGCCT 2 cut(s) 1032, 1113
PciSI GCTCTTC 1 cut(s) 483
PcsI WCGNNNNNNNCGW 1 cut(s) 1634
PfeI GAWTC 1 cut(s) 647
PflFI GACNNNGTC 2 cut(s) 229, 337
PfoI TCCNGGA 1 cut(s) 1467
PleI GAGTC 3 cut(s) 832, 904, 1528
PluTI GGCGCC 1 cut(s) 450
PpsI GAGTC 3 cut(s) 832, 904, 1528
Ppu21I YACGTR 1 cut(s) 34
PpuMI RGGWCCY 1 cut(s) 785
PshAI GACNNNNGTC 1 cut(s) 283
Psp5II RGGWCCY 1 cut(s) 785
Psp6I CCWGG 6 cut(s) 214, 442, 1054, 1363, 1424, 1467
PspEI GGTNACC 1 cut(s) 1435
PspFI CCCAGC 6 cut(s) 79, 133, 160, 241, 295, 1387
PspGI CCWGG 6 cut(s) 214, 442, 1054, 1363, 1424, 1467
PspPPI RGGWCCY 1 cut(s) 785
PstI CTGCAG 1 cut(s) 1594
PsyI GACNNNGTC 2 cut(s) 229, 337
PvuII CAGCTG 2 cut(s) 1595, 1622
RruI TCGCGA 2 cut(s) 594, 851
RsaI GTAC 2 cut(s) 1301, 1644
RsaNI GTAC 2 cut(s) 1300, 1643
Rsr2I CGGWCCG 3 cut(s) 143, 197, 278
RsrII CGGWCCG 3 cut(s) 143, 197, 278
SalI GTCGAC 5 cut(s) 127, 154, 316, 391, 830
SapI GCTCTTC 1 cut(s) 483
Sau3AI GATC 5 cut(s) 10, 532, 750, 1375, 1672
SchI GAGTC 3 cut(s) 833, 905, 1529
ScrFI CCNGG 8 cut(s) 216, 374, 444, 775, 1056, 1365, 1426, 1469
SduI GDGCHC 1 cut(s) 1740
SfaNI GCATC 3 cut(s) 700, 1145, 1634
SfcI CTRYAG 1 cut(s) 1590
SfoI GGCGCC 1 cut(s) 448
SgrDI CGTCGACG 2 cut(s) 391, 830
SinI GGWCC 6 cut(s) 143, 197, 278, 369, 785, 857
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
Sse9I AATT 5 cut(s) 455, 461, 681, 1003, 1530
SseBI AGGCCT 2 cut(s) 1032, 1113
SspDI GGCGCC 1 cut(s) 446
SspMI CTAG 2 cut(s) 288, 1632
StuI AGGCCT 2 cut(s) 1032, 1113
StyD4I CCNGG 8 cut(s) 214, 372, 442, 773, 1054, 1363, 1424, 1467
StyI CCWWGG 3 cut(s) 84, 246, 365
TaaI ACNGT 4 cut(s) 1078, 1175, 1194, 1717
TaiI ACGT 3 cut(s) 36, 230, 398
TaqI TCGA 8 cut(s) 128, 155, 317, 392, 638, 831, 1505, 1563
TaqII GACCGA 3 cut(s) 185, 539, 1651
TasI AATT 5 cut(s) 455, 461, 681, 1003, 1530
TatI WGTACW 1 cut(s) 1299
TauI GCSGC 7 cut(s) 67, 121, 151, 175, 256, 310, 739
TfiI GAWTC 1 cut(s) 647
TscAI CASTG 2 cut(s) 1045, 1501
TseFI GTSAC 2 cut(s) 34, 358
TseI GCWGC 7 cut(s) 53, 403, 430, 1330, 1589, 1592, 1622
Tsp45I GTSAC 2 cut(s) 34, 358
TspDTI ATGAA 3 cut(s) 1186, 1538, 1549
TspGWI ACGGA 2 cut(s) 378, 817
TspRI CASTG 2 cut(s) 1045, 1501
Tth111I GACNNNGTC 2 cut(s) 229, 337
Vha464I CTTAAG 1 cut(s) 815
VpaK11BI GGWCC 6 cut(s) 143, 197, 278, 369, 785, 857
XapI RAATTY 3 cut(s) 455, 461, 1003
XceI RCATGY 1 cut(s) 1319
XmiI GTMKAC 5 cut(s) 128, 155, 317, 392, 831
XspI CTAG 2 cut(s) 288, 1632
ZraI GACGTC 1 cut(s) 228
Zsp2I ATGCAT 1 cut(s) 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.