FvH4_5g21970
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000163
Physical Location & Seq
Reverse (-)
13337197 .. 13341734
4538 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21970.t1

Sequence Viewer

Length: 2934 bp
ATGATACCAAAAAATGTTCAAGGTGATACCTCTTCAGCTGAAAACTCTGGTACTCCTGATAATAATATGCTCTCAACTGAGATGAGAAATAATGATGATGCTGAGAATCCTTTGATTATTGATAATAGTGAAACATGTCCTGCCCAGATAGTTGTGTCTAAAGAACCTGCAAATATTGTCTCTTATACTGATGAGCCATACATCCCAATTTCAGAAGTCCATATTGATATTGGTCCACCCTCAACTGCCTCTCAGAACACAATGACCTCATTTATTCCCTTGTCCAACCTAAACAGTAATGATCATCCAATGATAACCAGAAGCAAGCATGGGATTTATAAGAAGAAGGTGTTCTTTGCAGCTAATACTGCAATTAAAATGACAGCAGATAAGGAACCACATTCGTTCAAACAGGCTCTTCAAGTTTCAGAATGGAAACAGGCTATGAAGGATGAATATGATGCTCTCATTCAGCAAGGAACTTGGTTTTTAGTTCCCCCTCAACAACATAAGAACCTGGCAAGCTGTAAGTGGATTTTTAAGTTAAAGAAAAATCCCGATGGGTCAATTGCAAGGCACAAGGCAAGATTGGTTGCTAGAGGTTTCAGTCAAGAAGAAGGAGTGGATTATGATGAAACTTTTAGTCCAGTAGTGAGGCATACCACAGTTAGATTATTTCTAGCTCTTGCAGCACAGTTCAACTGGAAACTACATCAGATGGATGTGAAAAACGCCTTCCTTCATGGGGTACTTAAAGAAGAGGTCTACATGTCACAACCACAAGGTTTTGAGGACTCTGAGCATCCACATTATGTGTGCAGATTAGTTAAATCCCTCTATGGCTTAAAACAAGCTCCTAGAGCTTGGAATGAAAGATTTACCTCTTTTCTTCCATCTCTGGGATTTAAAGGCTCACACTCAGATCCCTCTCTCTTTGTCAAAGTTTTTGGGTCCTCTGTGGTCTATTTATTACTCTATGTTGATGATATCATTATGACAGGGAAAGATGAAACTGAGATTGGAGTAGTGAAACTTGCCTTGCAGTTAGAATTTGACATGAAAGATCTAGGCAGGCTGCATTACTTTCTAGGACTGCAAATTGAATATCCACCATCTGGAGGGATCTTTATATCCCAATCCAAATATGCTTCTGAAGTGATTATTAAAGCAGGAATGATTGGTAAGAAGAGCTGCTCAACTCCTTGCTTACCTTATGCCAAACTAATAAGAGATGATGGACCATTATTCAGTGATGTTACACACGATAGAAGCATTGTTGGATGCCTTCAATATTTGACTTTCTCAAGACCAGACATTGCATACAGTGTTAATACTGTGTGTCAGTTCATGCAAGCTCCAACTGAAGCTCACTATTCTGCAGTCAAACGAATCCTCAGATACTTAATTGGTATTGTGAATTATGGACTAACATATAAACCAGCTCCTGACCCTGTTGAACTCAAGGCTTTTTTAGATGCAGATTGGGCAGGAGATCCCAATGACAGAAGATCAACCACTGGCTTTGTAATTTATCTTGGAAATTGTCCCATTTCTTGGTGTTCCAAGAAACAACACTCAGTCTCTCGGTCTTCTACTGAGGCTGAATATAGGGCCATGACAGACACTGCAAGTGAGATATTTTGGCTGAGACACCTGCTATCAGACATCTATGTGAGTTTACCACGTCCTCCTTTTCTACACTGTGATAATGCATCCGCTCTTTCTCTTGTCTCAAATCCCATTCATAAGTCAAAGTGTAAACATGTGGAGGTTGATATTCATTTTACAAGAGAGAAGGTTGTTAAGGGTGAGCTCACTCTTCAGTTTGTTCCCTCACTCGAACAGTTTGCTGATATCTTCACCAAAGGCCTCAGTACTGCACATTTTGAATACCTATGCTGCAACCTAGTGCTCGAATCAATGGCTTCTGGTAAATGGTCGAAACGTGAACGATCCCCAGAACCACCTTCATTTAGAGCATCAAAGCAACTGAAAATGTCCTTTAAAAACCAAACCAAGGTCGCAATGGGGATCACAGGACAATTGCTTCGGACAGAAGGTAAGGACAAGAACGTGGTCTACTCGCCTTTGTCTATTCATGTGGTTTTGAGCATGATAGCTTCTGGGGCGTCAAAGGGTCCAAACATGGACCAGTTGCTCTATTTCCTCAGGGCCAAGTCCGTCAGTGAGCTCAACGACCTCGCCTCCAGTTTGGTCCCTTTGGTCTTTGCAGACGGATCGCCAAGTGGAGGTCCTCGCTTGTCATTCGCCAATGGCGTTTGGTTAGAGGATTCTGTCTCTATCAAGACTCCTTTCAAAGAAGCGGTGGACTTGTCATACAAGGCATTATTGAAGCAAGTAGATTTTAAGACCAAATCAGAAGAAGTGAGATGTGAAGTGAATGCATGGGTGGAGAAGCAGACCAATGGCCTTATCAAAGACATTATTCCTCCAGGGTTGGTTGATAGATCAACAGATATAATCCTTGAATATGCCTTATACTTCAAAGGAGCTTGGGACAAGAATGTGAATTTTCACGCATCAAATACAAGAGAGTGGGGTTTTCATCTTCAGGATGGGAGGTCAGTTAAGGCACTGTTCATGACCAGTGGGGGGACTCATTATGTGAGTGTCTTTGACGGCTTCAAAGTGCTAAGGCTTCCTTATGAACAGGGTCGAGATTGCAAAAGGCGGTTCTCTATGTGTGTATTTCTTCCAAATGAAAGAGATGGGTTGCCAGCTTTGGTTGAGAGAGTTTCTACCGATTTTGGGTTTCTAGATAGACATATTCCCAGAGAACGTGTTCGGGTTGGCAGATTTTTAATCCCAAAGTTTAAGATCTCTTCGGACTTTGACTGCATCAAAGTTCTGGAACCTCTAGGATTGAAGCTGCCTTGTTTGATAGAAATGGTGGAAGGCAGCCTCCATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

978

Amino Acids

109.86

Weight (kDa)

8.22

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 161 - 403 5.4e-74 Reverse transcriptase (RNA-dependent DNA polymerase)
Serpin PF00079 670 - 963 1.6e-54 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64020
fragaria_vesca FvH4_1g24600 FvH4_1g24640 FvH4_2g15000 FvH4_3g03700 FvH4_3g03710 FvH4_3g09790 FvH4_3g09790 FvH4_3g09810 FvH4_3g09811 FvH4_3g23720 FvH4_3g32870 FvH4_3g32890 FvH4_3g37550 FvH4_3g37560 FvH4_5g01890 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g21100 FvH4_5g21110 FvH4_5g21330 FvH4_5g21331 FvH4_5g21520 FvH4_5g21540 FvH4_5g21970 FvH4_6g01280
malus_domestica MD00G1084800.v1.1 MD00G1141300.v1.1 MD02G1102800.v1.1 MD02G1282200.v1.1 MD02G1282300.v1.1 MD03G1207900.v1.1 MD03G1230000.v1.1 MD04G1136400.v1.1 MD04G1136500.v1.1 MD04G1189000.v1.1 MD05G1248700.v1.1 MD05G1248800.v1.1 MD06G1030400.v1.1 MD10G1229200.v1.1 MD10G1229300.v1.1 MD15G1291400.v1.1 MD15G1431300.v1.1
prunus_persica Prupe.5G041800_v2.0.a1 Prupe.5G041900_v2.0.a1 Prupe.5G042000_v2.0.a1 Prupe.5G042100_v2.0.a1 Prupe.5G042200_v2.0.a1
pyrus_communis pycom02g14630 pycom02g24090 pycom04g12410 pycom04g16760 pycom05g22550 pycom10g19280 pycom12g05670 pycom15g25490 pycom15g38110
rosa_chinensis RchiOBHm_Chr2g0122001 RchiOBHm_Chr2g0163171 RchiOBHm_Chr2g0163181 RchiOBHm_Chr3g0455201 RchiOBHm_Chr5g0005751 RchiOBHm_Chr5g0015501 RchiOBHm_Chr5g0015511 RchiOBHm_Chr5g0015521 RchiOBHm_Chr5g0015531 RchiOBHm_Chr5g0060021 RchiOBHm_Chr7g0187971 RchiOBHm_Chr7g0207951 RchiOBHm_Chr7g0208671 RchiOBHm_Chr7g0208691 RchiOBHm_Chr7g0208941 RchiOBHm_Chr7g0208951 RchiOBHm_Chr7g0208961
rosa_laevigata RLG00000003132 RLG00000003177 RLG00000003178 RLG00000003179 RLG00000003180 RLG00000003201 RLG00000003202 RLG00000003270 RLG00000004739 RLG00000004741 RLG00000004744 RLG00000021379 RLG00000025378 RLG00000031323 RLG00000031324 RLG00000032205 RLG00000032206 RLG00000035339
rosa_multiflora Rmu_co8189380.1_g000001 Rmu_co8284591.1_g000001 Rmu_co8360283.1_g000002 Rmu_co8379053.1_g000001 Rmu_sc0000493.1_g000007 Rmu_sc0000532.1_g000030 Rmu_sc0000532.1_g000031 Rmu_sc0000570.1_g000033 Rmu_sc0000905.1_g000012 Rmu_sc0001730.1_g000002 Rmu_sc0002045.1_g000039 Rmu_sc0003064.1_g000001 Rmu_sc0003064.1_g000002 Rmu_sc0003064.1_g000006 Rmu_sc0003880.1_g000012 Rmu_sc0004103.1_g000003 Rmu_sc0005082.1_g000007 Rmu_sc0011272.1_g000002 Rmu_sc0015523.1_g000021 Rmu_sc0015523.1_g000022 Rmu_sc0019960.1_g000007 Rmu_sc0029514.1_g000001 Rmu_ssc0000422.1_g000010
rosa_roxburghii Rroxscaffold_1G00013660 Rroxscaffold_1G00060980 Rroxscaffold_1G00060990 Rroxscaffold_1G00061000 Rroxscaffold_1G00061010 Rroxscaffold_2G00087490 Rroxscaffold_2G00122080 Rroxscaffold_2G00123490 Rroxscaffold_3G00249930 Rroxscaffold_3G00249940 Rroxscaffold_3G00249950 Rroxscaffold_3G00249980 Rroxscaffold_3G00250310 Rroxscaffold_3G00250320 Rroxscaffold_3G00266850 Rroxscaffold_3G00266870 Rroxscaffold_3G00266910 Rroxscaffold_6G00424480
rosa_rugosa Rorug02G0228900 Rorug02G0236000 Rorug02G0501100 Rorug03G0004700 Rorug03G0004700 Rorug03G0004800 Rorug04G0417800 Rorug05G0027800 Rorug05G0027900 Rorug05G0028000 Rorug05G0327700 Rorug05G0384800 Rorug06G0486000 Rorug06G0486100 Rorug06G0486200 Rorug06G0486200 Rorug06G0486300 Rorug06G0486400 Rorug06G0486500 Rorug07G0100400 Rorug07G0105400 Rorug07G0105400 Rorug07G0107300 Rorug07G0107400 Rorug07G0107500 Rorug07G0113300
rosa_samantha Rh7AG091300 Rh7BG092800 Rh7CG091600 Rh7CG257500 Rh7DG093500
rosa_wichuraiana Rw0G001260 Rw0G017680 Rw2G023470 Rw3G005130 Rw5G004560 Rw5G010480 Rw7G020400 Rw7G020410 Rw7G020540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
AarI CACCTGC 1 cut(s) 1662
Acc36I ACCTGC 2 cut(s) 175, 1662
AccB7I CCANNNNNTGG 2 cut(s) 1115, 1554
AccBSI CCGCTC 1 cut(s) 1718
AccI GTMKAC 2 cut(s) 765, 2079
AciI CCGC 3 cut(s) 1716, 2324, 2692
AclWI GGATC 6 cut(s) 917, 1130, 1487, 1947, 2039, 2245
AcsI RAATTY 2 cut(s) 1049, 2530
AcuI CTGAAG 5 cut(s) 18, 1173, 1383, 1805, 2555
AcyI GRCGYC 1 cut(s) 2129
AfaI GTAC 3 cut(s) 52, 750, 1876
AfiI CCNNNNNNNGG 9 cut(s) 745, 899, 1115, 1118, 1554, 2017, 2249, 2613, 2769
AflIII ACRYGT 4 cut(s) 134, 768, 1762, 2800
AjiI CACGTC 1 cut(s) 1685
AjnI CCWGG 2 cut(s) 516, 2452
AjuI GAANNNNNNNTTGG 2 cut(s) 1002, 1034
Alw21I GWGCWC 3 cut(s) 1815, 1914, 2193
Alw26I GTCTC 5 cut(s) 184, 1585, 1642, 1735, 2302
AlwI GGATC 6 cut(s) 917, 1130, 1487, 1947, 2039, 2245
AlwNI CAGNNNCTG 2 cut(s) 1445, 1625
AoxI GGCC 4 cut(s) 1611, 1867, 2172, 2428
ApeKI GCWGC 7 cut(s) 359, 689, 1075, 1191, 1899, 2890, 2919
ApoI RAATTY 2 cut(s) 1049, 2530
ArsI GACNNNNNNTTYG 2 cut(s) 2031, 2063
Asp700I GAANNNNTTC 2 cut(s) 350, 2802
AspS9I GGNCC 9 cut(s) 233, 951, 1238, 1611, 2138, 2149, 2172, 2215, 2252
AsuHPI GGTGA 3 cut(s) 35, 1820, 1852
AvaII GGWCC 7 cut(s) 233, 951, 1238, 2138, 2149, 2215, 2252
AxyI CCTNAGG 1 cut(s) 2168
BanII GRGCYC 2 cut(s) 1815, 2193
BbsI GAAGAC 1 cut(s) 1581
Bbv12I GWGCWC 3 cut(s) 1815, 1914, 2193
BbvI GCAGC 6 cut(s) 371, 701, 1062, 1178, 1886, 2877
BccI CCATC 7 cut(s) 554, 712, 901, 1120, 1229, 2570, 2723
BceAI ACGGC 1 cut(s) 2656
BcgI CGANNNNNNTGC 4 cut(s) 1829, 1863, 2220, 2254
BciT130I CCWGG 2 cut(s) 518, 2454
BclI TGATCA 1 cut(s) 301
BcoDI GTCTC 5 cut(s) 184, 1585, 1642, 1735, 2302
BfaI CTAG 8 cut(s) 597, 680, 858, 1067, 1088, 1907, 2777, 2879
BfmI CTRYAG 1 cut(s) 1377
BfuAI ACCTGC 2 cut(s) 175, 1662
BglII AGATCT 2 cut(s) 1063, 2838
BisI GCNGC 7 cut(s) 360, 690, 1076, 1192, 1900, 2891, 2920
BlsI GCNGC 7 cut(s) 361, 691, 1077, 1193, 1901, 2892, 2921
BmcAI AGTACT 1 cut(s) 1876
Bme1390I CCNGG 2 cut(s) 518, 2454
Bme18I GGWCC 7 cut(s) 233, 951, 1238, 2138, 2149, 2215, 2252
BmgBI CACGTC 1 cut(s) 1685
BmgT120I GGNCC 9 cut(s) 233, 951, 1238, 1611, 2138, 2149, 2172, 2215, 2252
BmiI GGNNCC 5 cut(s) 396, 952, 2139, 2217, 2874
BmrFI CCNGG 2 cut(s) 518, 2454
BmsI GCATC 9 cut(s) 88, 451, 811, 1271, 1465, 1721, 1988, 2549, 2868
BpiI GAAGAC 1 cut(s) 1581
BpmI CTGGAG 3 cut(s) 1137, 2191, 2436
Bpu10I CCTNAGC 1 cut(s) 2654
BpuEI CTTGAG 2 cut(s) 1288, 1445
BsaHI GRCGYC 1 cut(s) 2129
BsaJI CCNNGG 2 cut(s) 2016, 2453
BsaXI ACNNNNNCTCC 2 cut(s) 2189, 2219
Bsc4I CCNNNNNNNGG 9 cut(s) 745, 899, 1115, 1118, 1554, 2017, 2249, 2613, 2769
Bse1I ACTGG 6 cut(s) 647, 707, 1522, 2152, 2208, 2607
Bse21I CCTNAGG 1 cut(s) 2168
Bse3DI GCAATG 2 cut(s) 1314, 2031
BseBI CCWGG 2 cut(s) 518, 2454
BseDI CCNNGG 2 cut(s) 2016, 2453
BseGI GGATG 8 cut(s) 201, 304, 457, 727, 802, 1286, 1712, 2581
BseLI CCNNNNNNNGG 9 cut(s) 745, 899, 1115, 1118, 1554, 2017, 2249, 2613, 2769
BseMI GCAATG 2 cut(s) 1314, 2031
BseNI ACTGG 6 cut(s) 647, 707, 1522, 2152, 2208, 2607
BseXI GCAGC 6 cut(s) 371, 701, 1062, 1178, 1886, 2877
BsgI GTGCAG 2 cut(s) 838, 1863
BshFI GGCC 4 cut(s) 1613, 1869, 2174, 2430
BsiHKAI GWGCWC 3 cut(s) 1815, 1914, 2193
BslFI GGGAC 4 cut(s) 1530, 2201, 2531, 2629
BslI CCNNNNNNNGG 9 cut(s) 745, 899, 1115, 1118, 1554, 2017, 2249, 2613, 2769
BsmAI GTCTC 5 cut(s) 184, 1585, 1642, 1735, 2302
BsmFI GGGAC 4 cut(s) 1530, 2201, 2531, 2629
BsmI GAATGC 1 cut(s) 2407
BsnI GGCC 4 cut(s) 1613, 1869, 2174, 2430
Bsp1286I GDGCHC 3 cut(s) 1815, 1914, 2193
BspACI CCGC 3 cut(s) 1716, 2324, 2692
BspANI GGCC 4 cut(s) 1613, 1869, 2174, 2430
BspHI TCATGA 1 cut(s) 2601
BspLI GGNNCC 5 cut(s) 396, 952, 2139, 2217, 2874
BspMAI CTGCAG 1 cut(s) 1381
BspMI ACCTGC 2 cut(s) 175, 1662
BspPI GGATC 6 cut(s) 917, 1130, 1487, 1947, 2039, 2245
BspQI GCTCTTC 2 cut(s) 423, 1181
BsrBI CCGCTC 1 cut(s) 1718
BsrDI GCAATG 2 cut(s) 1314, 2031
BsrI ACTGG 6 cut(s) 647, 707, 1522, 2152, 2208, 2607
BssECI CCNNGG 2 cut(s) 2016, 2453
BssNI GRCGYC 1 cut(s) 2129
BssT1I CCWWGG 1 cut(s) 2016
Bst2UI CCWGG 2 cut(s) 518, 2454
Bst4CI ACNGT 8 cut(s) 296, 667, 696, 1325, 1336, 1703, 1845, 2598
Bst6I CTCTTC 6 cut(s) 37, 423, 753, 1181, 1824, 2848
BstACI GRCGYC 1 cut(s) 2129
BstC8I GCNNGC 5 cut(s) 326, 523, 1073, 1353, 2739
BstF5I GGATG 8 cut(s) 201, 304, 457, 727, 802, 1286, 1712, 2581
BstMAI GTCTC 5 cut(s) 184, 1585, 1642, 1735, 2302
BstMWI GCNNNNNNNGC 5 cut(s) 368, 689, 860, 1484, 2126
BstNI CCWGG 2 cut(s) 518, 2454
BstNSI RCATGY 3 cut(s) 138, 772, 1766
BstSCI CCNGG 2 cut(s) 516, 2452
BstSFI CTRYAG 1 cut(s) 1377
BstV1I GCAGC 6 cut(s) 371, 701, 1062, 1178, 1886, 2877
BstV2I GAAGAC 1 cut(s) 1581
BstX2I RGATCY 5 cut(s) 922, 1063, 1122, 1492, 2838
BstYI RGATCY 5 cut(s) 922, 1063, 1122, 1492, 2838
Bsu36I CCTNAGG 1 cut(s) 2168
BsuRI GGCC 4 cut(s) 1613, 1869, 2174, 2430
BtrI CACGTC 1 cut(s) 1685
BtsCI GGATG 8 cut(s) 201, 304, 457, 727, 802, 1286, 1712, 2581
BtsI GCAGTG 1 cut(s) 1623
BtsIMutI CAGTG 8 cut(s) 1255, 1330, 1515, 1623, 1699, 2191, 2594, 2614
BveI ACCTGC 2 cut(s) 175, 1662
Cac8I GCNNGC 5 cut(s) 326, 523, 1073, 1353, 2739
CaiI CAGNNNCTG 2 cut(s) 1445, 1625
CciI TCATGA 1 cut(s) 2601
Cfr13I GGNCC 9 cut(s) 233, 951, 1238, 1611, 2138, 2149, 2172, 2215, 2252
CseI GACGC 1 cut(s) 2118
Csp6I GTAC 3 cut(s) 51, 749, 1875
CspCI CAANNNNNGTGG 2 cut(s) 1504, 1539
CviQI GTAC 3 cut(s) 51, 749, 1875
DraI TTTAAA 2 cut(s) 907, 2005
Eam1104I CTCTTC 6 cut(s) 37, 423, 753, 1181, 1824, 2848
EarI CTCTTC 6 cut(s) 37, 423, 753, 1181, 1824, 2848
Ecl136II GAGCTC 2 cut(s) 1813, 2191
Eco130I CCWWGG 1 cut(s) 2016
Eco147I AGGCCT 1 cut(s) 1869
Eco24I GRGCYC 2 cut(s) 1815, 2193
Eco32I GATATC 2 cut(s) 988, 1855
Eco47I GGWCC 7 cut(s) 233, 951, 1238, 2138, 2149, 2215, 2252
Eco53kI GAGCTC 2 cut(s) 1813, 2191
Eco57I CTGAAG 5 cut(s) 18, 1173, 1383, 1805, 2555
Eco81I CCTNAGG 1 cut(s) 2168
EcoICRI GAGCTC 2 cut(s) 1813, 2191
EcoO109I RGGNCCY 2 cut(s) 951, 2252
EcoRII CCWGG 2 cut(s) 516, 2452
EcoRV GATATC 2 cut(s) 988, 1855
EcoT14I CCWWGG 1 cut(s) 2016
EcoT22I ATGCAT 2 cut(s) 1714, 2407
EcoT38I GRGCYC 2 cut(s) 1815, 2193
ErhI CCWWGG 1 cut(s) 2016
FalI AAGNNNNNCTT 4 cut(s) 338, 370, 2647, 2679
FaqI GGGAC 4 cut(s) 1530, 2201, 2531, 2629
FbaI TGATCA 1 cut(s) 301
FblI GTMKAC 2 cut(s) 765, 2079
Fnu4HI GCNGC 7 cut(s) 360, 690, 1076, 1192, 1900, 2891, 2920
FokI GGATG 8 cut(s) 188, 291, 464, 734, 789, 1293, 1699, 2588
FriOI GRGCYC 2 cut(s) 1815, 2193
Fsp4HI GCNGC 7 cut(s) 360, 690, 1076, 1192, 1900, 2891, 2920
FspBI CTAG 8 cut(s) 597, 680, 858, 1067, 1088, 1907, 2777, 2879
GluI GCNGC 7 cut(s) 360, 690, 1076, 1192, 1900, 2891, 2920
GsuI CTGGAG 3 cut(s) 1137, 2191, 2436
HaeIII GGCC 4 cut(s) 1613, 1869, 2174, 2430
HgaI GACGC 1 cut(s) 2118
Hin1I GRCGYC 1 cut(s) 2129
HinfI GANTC 7 cut(s) 106, 794, 1389, 1916, 2291, 2308, 2617
HphI GGTGA 3 cut(s) 35, 1820, 1852
Hpy166II GTNNAC 7 cut(s) 236, 766, 1679, 1760, 1949, 2080, 2329
Hpy8I GTNNAC 7 cut(s) 236, 766, 1679, 1760, 1949, 2080, 2329
HpyCH4III ACNGT 8 cut(s) 296, 667, 696, 1325, 1336, 1703, 1845, 2598
HpyCH4IV ACGT 4 cut(s) 1684, 1945, 2073, 2800
HpyF10VI GCNNNNNNNGC 5 cut(s) 368, 689, 860, 1484, 2126
HpySE526I ACGT 4 cut(s) 1684, 1945, 2073, 2800
Hsp92I GRCGYC 1 cut(s) 2129
Ksp22I TGATCA 1 cut(s) 301
LguI GCTCTTC 2 cut(s) 423, 1181
LmnI GCTCC 4 cut(s) 859, 1360, 1447, 2510
Lsp1109I GCAGC 6 cut(s) 371, 701, 1062, 1178, 1886, 2877
LweI GCATC 9 cut(s) 88, 451, 811, 1271, 1465, 1721, 1988, 2549, 2868
MaeI CTAG 8 cut(s) 597, 680, 858, 1067, 1088, 1907, 2777, 2879
MaeII ACGT 4 cut(s) 1684, 1945, 2073, 2800
MaeIII GTNAC 2 cut(s) 771, 1255
MbiI CCGCTC 1 cut(s) 1718
MfeI CAATTG 2 cut(s) 567, 2042
MflI RGATCY 5 cut(s) 922, 1063, 1122, 1492, 2838
MhlI GDGCHC 3 cut(s) 1815, 1914, 2193
MlyI GAGTC 3 cut(s) 788, 2302, 2611
MmeI TCCRAC 3 cut(s) 309, 1258, 1382
Mph1103I ATGCAT 2 cut(s) 1714, 2407
MroXI GAANNNNTTC 2 cut(s) 350, 2802
MslI CAYNNNNRTG 1 cut(s) 1670
MspA1I CMGCKG 1 cut(s) 38
MspR9I CCNGG 2 cut(s) 518, 2454
MunI CAATTG 2 cut(s) 567, 2042
Mva1269I GAATGC 1 cut(s) 2407
MvaI CCWGG 2 cut(s) 518, 2454
MwoI GCNNNNNNNGC 5 cut(s) 368, 689, 860, 1484, 2126
NlaIV GGNNCC 5 cut(s) 396, 952, 2139, 2217, 2874
NmuCI GTSAC 1 cut(s) 771
NsiI ATGCAT 2 cut(s) 1714, 2407
NspI RCATGY 3 cut(s) 138, 772, 1766
PagI TCATGA 1 cut(s) 2601
PaqCI CACCTGC 1 cut(s) 1662
PceI AGGCCT 1 cut(s) 1869
PciI ACATGT 3 cut(s) 134, 768, 1762
PciSI GCTCTTC 2 cut(s) 423, 1181
PcsI WCGNNNNNNNCGW 1 cut(s) 2274
PctI GAATGC 1 cut(s) 2407
PdmI GAANNNNTTC 2 cut(s) 350, 2802
PfeI GAWTC 4 cut(s) 106, 1389, 1916, 2291
PflMI CCANNNNNTGG 2 cut(s) 1115, 1554
PkrI GCNGC 7 cut(s) 361, 691, 1077, 1193, 1901, 2892, 2921
PleI GAGTC 3 cut(s) 788, 2302, 2611
PpsI GAGTC 3 cut(s) 788, 2302, 2611
PpuMI RGGWCCY 2 cut(s) 951, 2252
PscI ACATGT 3 cut(s) 134, 768, 1762
PsiI TTATAA 1 cut(s) 339
Psp124BI GAGCTC 2 cut(s) 1815, 2193
Psp5II RGGWCCY 2 cut(s) 951, 2252
Psp6I CCWGG 2 cut(s) 516, 2452
PspGI CCWGG 2 cut(s) 516, 2452
PspN4I GGNNCC 5 cut(s) 396, 952, 2139, 2217, 2874
PspPI GGNCC 9 cut(s) 233, 951, 1238, 1611, 2138, 2149, 2172, 2215, 2252
PspPPI RGGWCCY 2 cut(s) 951, 2252
PsrI GAACNNNNNNTAC 2 cut(s) 2063, 2095
PstI CTGCAG 1 cut(s) 1381
PstNI CAGNNNCTG 2 cut(s) 1445, 1625
PsuI RGATCY 5 cut(s) 922, 1063, 1122, 1492, 2838
PvuII CAGCTG 1 cut(s) 38
RsaI GTAC 3 cut(s) 52, 750, 1876
RsaNI GTAC 3 cut(s) 51, 749, 1875
RseI CAYNNNNRTG 1 cut(s) 1670
SacI GAGCTC 2 cut(s) 1815, 2193
SapI GCTCTTC 2 cut(s) 423, 1181
SatI GCNGC 7 cut(s) 360, 690, 1076, 1192, 1900, 2891, 2920
Sau96I GGNCC 9 cut(s) 233, 951, 1238, 1611, 2138, 2149, 2172, 2215, 2252
ScaI AGTACT 1 cut(s) 1876
SchI GAGTC 3 cut(s) 788, 2302, 2611
ScrFI CCNGG 2 cut(s) 518, 2454
SduI GDGCHC 3 cut(s) 1815, 1914, 2193
SfaNI GCATC 9 cut(s) 88, 451, 811, 1271, 1465, 1721, 1988, 2549, 2868
SfcI CTRYAG 1 cut(s) 1377
SinI GGWCC 7 cut(s) 233, 951, 1238, 2138, 2149, 2215, 2252
SmiMI CAYNNNNRTG 1 cut(s) 1670
SmlI CTYRAG 2 cut(s) 1303, 1460
SmoI CTYRAG 2 cut(s) 1303, 1460
SseBI AGGCCT 1 cut(s) 1869
SsiI CCGC 3 cut(s) 1716, 2324, 2692
SspI AATATT 2 cut(s) 175, 1292
SspMI CTAG 8 cut(s) 597, 680, 858, 1067, 1088, 1907, 2777, 2879
SstI GAGCTC 2 cut(s) 1815, 2193
StuI AGGCCT 1 cut(s) 1869
StyD4I CCNGG 2 cut(s) 516, 2452
StyI CCWWGG 1 cut(s) 2016
TaaI ACNGT 8 cut(s) 296, 667, 696, 1325, 1336, 1703, 1845, 2598
TaiI ACGT 4 cut(s) 1687, 1948, 2076, 2803
TaqI TCGA 4 cut(s) 1839, 1914, 1940, 2677
TaqII GACCGA 1 cut(s) 1575
TatI WGTACW 1 cut(s) 1874
TfiI GAWTC 4 cut(s) 106, 1389, 1916, 2291
TscAI CASTG 8 cut(s) 1255, 1330, 1522, 1630, 1706, 2191, 2601, 2614
TseFI GTSAC 1 cut(s) 771
TseI GCWGC 7 cut(s) 359, 689, 1075, 1191, 1899, 2890, 2919
Tsp45I GTSAC 1 cut(s) 771
TspGWI ACGGA 2 cut(s) 2170, 2250
TspRI CASTG 8 cut(s) 1255, 1330, 1522, 1630, 1706, 2191, 2601, 2614
Van91I CCANNNNNTGG 2 cut(s) 1115, 1554
VpaK11BI GGWCC 7 cut(s) 233, 951, 1238, 2138, 2149, 2215, 2252
XapI RAATTY 2 cut(s) 1049, 2530
XbaI TCTAGA 1 cut(s) 2776
XceI RCATGY 3 cut(s) 138, 772, 1766
XcmI CCANNNNNNNNNTGG 2 cut(s) 227, 2023
XmiI GTMKAC 2 cut(s) 765, 2079
XmnI GAANNNNTTC 2 cut(s) 350, 2802
XspI CTAG 8 cut(s) 597, 680, 858, 1067, 1088, 1907, 2777, 2879
ZrmI AGTACT 1 cut(s) 1876
Zsp2I ATGCAT 2 cut(s) 1714, 2407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.