Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0000163

Physical Location & Seq

Genomic Coordinates Forward (+)

12994505 .. 12996524

Gene Structure 2020 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g21540.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 882 bp

ATGAAGCAGCTGCTTTCGACTGAAGGCAAGGACAGGAATATGGTGTACTCACCGCTGTCCATCCACATTGTTCTTAGCCTTATAGCGGCCGGGTCAGACGGTCCTACACAAGACCAGTTGCTCGCCTTCCTTAAGTCCAAGTCCACCCAGGAACTCAACGATCTAGCCTCCACCCTCGTGCCTCTGTTCTTTGTGGAAGGATCACCAAATGGCGGGCCTGGTTTGTCATTCACCAATGGTGTTTGGATTGACAAGTCTCTCCCAGTCAAGCCTTCTTTCAAACGGGTAGTGGATACTGCTTACAAGGCTGCCTTAAAGCGAGTGGATTTCGAGCACAAGCCTGATGAAGTTAGAAGTGAAGTGAACTCATGGGCCGAGAAGGAGACTAATGGCCGGATCAAAAGTATTCTTGCTCCTGGCTCAGTTAACAGCCAAACTAAGCTCCTTCTCGCCAATGCATTATACTTTAAAGGAGATTGGGAGGACAAGTTTGATGCATCAGGAACGAAAGAATATGATTTCCACCTTCTCATTGGGAGCTCAGTTAAGACTCCTTTCATGACAAGGTGGATAACAAGTTGGGGAAGGCGGTATATAAGTGTTTTTGACAGTTTCAAAGTCTTGAAACTCCCTTACGAACAAGGTCAACATGGTGAGAAGTGTTTGTCCATGTATGTGTTTCTTCCAAACGATAGAGATGGATTGCCAGCTTTAGTTGAGAGATTTTCTTCCGAGTCTGGGTTCTTAGATGACCATCTTCCGCAGAAAACAGTTAACGTTGGTGCTTTTATGATGCCGAAGTTCAAGTTTTCTTCTAAATTCGAAGCTTCTGAGCTTCTGAAAACTGTAGGATTGAAGTTGCCTTTGTACCTGGAGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0004857 GO:0004866 GO:0004867 GO:0004869 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009892 GO:0010466 GO:0010605 GO:0010951 GO:0019222 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0043086 GO:0044092 GO:0044421 GO:0044424 GO:0044464 GO:0045861 GO:0048046 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko05146 map05146

Pfam Domains

Protein Families

Serpin

Protein Analysis

294

Amino Acids

32.8

Weight (kDa)

8.41

Isoelectric Point (pI)

31.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Serpin	PF00079	4 - 286	2.7e-63	Serpin (serine protease inhibitor)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000163)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64020
fragaria_vesca	FvH4_1g24600 FvH4_1g24640 FvH4_2g15000 FvH4_3g03700 FvH4_3g03710 FvH4_3g09790 FvH4_3g09790 FvH4_3g09810 FvH4_3g09811 FvH4_3g23720 FvH4_3g32870 FvH4_3g32890 FvH4_3g37550 FvH4_3g37560 FvH4_5g01890 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g21100 FvH4_5g21110 FvH4_5g21330 FvH4_5g21331 FvH4_5g21520 FvH4_5g21540 FvH4_5g21970 FvH4_6g01280
malus_domestica	MD00G1084800.v1.1 MD00G1141300.v1.1 MD02G1102800.v1.1 MD02G1282200.v1.1 MD02G1282300.v1.1 MD03G1207900.v1.1 MD03G1230000.v1.1 MD04G1136400.v1.1 MD04G1136500.v1.1 MD04G1189000.v1.1 MD05G1248700.v1.1 MD05G1248800.v1.1 MD06G1030400.v1.1 MD10G1229200.v1.1 MD10G1229300.v1.1 MD15G1291400.v1.1 MD15G1431300.v1.1
prunus_persica	Prupe.5G041800_v2.0.a1 Prupe.5G041900_v2.0.a1 Prupe.5G042000_v2.0.a1 Prupe.5G042100_v2.0.a1 Prupe.5G042200_v2.0.a1
pyrus_communis	pycom02g14630 pycom02g24090 pycom04g12410 pycom04g16760 pycom05g22550 pycom10g19280 pycom12g05670 pycom15g25490 pycom15g38110
rosa_chinensis	RchiOBHm_Chr2g0122001 RchiOBHm_Chr2g0163171 RchiOBHm_Chr2g0163181 RchiOBHm_Chr3g0455201 RchiOBHm_Chr5g0005751 RchiOBHm_Chr5g0015501 RchiOBHm_Chr5g0015511 RchiOBHm_Chr5g0015521 RchiOBHm_Chr5g0015531 RchiOBHm_Chr5g0060021 RchiOBHm_Chr7g0187971 RchiOBHm_Chr7g0207951 RchiOBHm_Chr7g0208671 RchiOBHm_Chr7g0208691 RchiOBHm_Chr7g0208941 RchiOBHm_Chr7g0208951 RchiOBHm_Chr7g0208961
rosa_laevigata	RLG00000003132 RLG00000003177 RLG00000003178 RLG00000003179 RLG00000003180 RLG00000003201 RLG00000003202 RLG00000003270 RLG00000004739 RLG00000004741 RLG00000004744 RLG00000021379 RLG00000025378 RLG00000031323 RLG00000031324 RLG00000032205 RLG00000032206 RLG00000035339
rosa_multiflora	Rmu_co8189380.1_g000001 Rmu_co8284591.1_g000001 Rmu_co8360283.1_g000002 Rmu_co8379053.1_g000001 Rmu_sc0000493.1_g000007 Rmu_sc0000532.1_g000030 Rmu_sc0000532.1_g000031 Rmu_sc0000570.1_g000033 Rmu_sc0000905.1_g000012 Rmu_sc0001730.1_g000002 Rmu_sc0002045.1_g000039 Rmu_sc0003064.1_g000001 Rmu_sc0003064.1_g000002 Rmu_sc0003064.1_g000006 Rmu_sc0003880.1_g000012 Rmu_sc0004103.1_g000003 Rmu_sc0005082.1_g000007 Rmu_sc0011272.1_g000002 Rmu_sc0015523.1_g000021 Rmu_sc0015523.1_g000022 Rmu_sc0019960.1_g000007 Rmu_sc0029514.1_g000001 Rmu_ssc0000422.1_g000010
rosa_roxburghii	Rroxscaffold_1G00013660 Rroxscaffold_1G00060980 Rroxscaffold_1G00060990 Rroxscaffold_1G00061000 Rroxscaffold_1G00061010 Rroxscaffold_2G00087490 Rroxscaffold_2G00122080 Rroxscaffold_2G00123490 Rroxscaffold_3G00249930 Rroxscaffold_3G00249940 Rroxscaffold_3G00249950 Rroxscaffold_3G00249980 Rroxscaffold_3G00250310 Rroxscaffold_3G00250320 Rroxscaffold_3G00266850 Rroxscaffold_3G00266870 Rroxscaffold_3G00266910 Rroxscaffold_6G00424480
rosa_rugosa	Rorug02G0228900 Rorug02G0236000 Rorug02G0501100 Rorug03G0004700 Rorug03G0004700 Rorug03G0004800 Rorug04G0417800 Rorug05G0027800 Rorug05G0027900 Rorug05G0028000 Rorug05G0327700 Rorug05G0384800 Rorug06G0486000 Rorug06G0486100 Rorug06G0486200 Rorug06G0486200 Rorug06G0486300 Rorug06G0486400 Rorug06G0486500 Rorug07G0100400 Rorug07G0105400 Rorug07G0105400 Rorug07G0107300 Rorug07G0107400 Rorug07G0107500 Rorug07G0113300
rosa_samantha	Rh7AG091300 Rh7BG092800 Rh7CG091600 Rh7CG257500 Rh7DG093500
rosa_wichuraiana	Rw0G001260 Rw0G017680 Rw2G023470 Rw3G005130 Rw5G004560 Rw5G010480 Rw7G020400 Rw7G020410 Rw7G020540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	5 cut(s)	53, 86, 213, 589, 761
AclI	AACGTT	1 cut(s)	777
AclWI	GGATC	2 cut(s)	208, 404
AcoI	YGGCCR	2 cut(s)	87, 391
AcsI	RAATTY	1 cut(s)	818
AcuI	CTGAAG	1 cut(s)	42
AfaI	GTAC	2 cut(s)	47, 869
AfiI	CCNNNNNNNGG	3 cut(s)	85, 212, 738
AflII	CTTAAG	1 cut(s)	131
AgsI	TTSAA	5 cut(s)	280, 616, 625, 805, 856
AjnI	CCWGG	4 cut(s)	147, 217, 415, 870
AleI	CACNNNNGTG	1 cut(s)	176
AluBI	AGCT	6 cut(s)	10, 442, 540, 710, 827, 835
AluI	AGCT	6 cut(s)	10, 442, 540, 710, 827, 835
Alw21I	GWGCWC	2 cut(s)	336, 542
Alw26I	GTCTC	2 cut(s)	261, 377
AlwI	GGATC	2 cut(s)	208, 404
AoxI	GGCC	4 cut(s)	87, 215, 372, 391
ApeKI	GCWGC	3 cut(s)	7, 10, 308
ApoI	RAATTY	1 cut(s)	818
AspS9I	GGNCC	3 cut(s)	101, 215, 372
AsuC2I	CCSGG	1 cut(s)	91
AsuHPI	GGTGA	4 cut(s)	42, 195, 223, 665
AsuII	TTCGAA	1 cut(s)	822
AvaII	GGWCC	1 cut(s)	101
BanII	GRGCYC	1 cut(s)	542
BauI	CACGAG	1 cut(s)	176
Bbv12I	GWGCWC	2 cut(s)	336, 542
BbvI	GCAGC	2 cut(s)	19, 295
BccI	CCATC	3 cut(s)	68, 692, 762
BciT130I	CCWGG	4 cut(s)	149, 219, 417, 872
BciVI	GTATCC	1 cut(s)	286
BcnI	CCSGG	1 cut(s)	91
BcoDI	GTCTC	2 cut(s)	261, 377
BfaI	CTAG	1 cut(s)	164
BfmI	CTRYAG	1 cut(s)	846
BfrI	CTTAAG	1 cut(s)	131
BfuI	GTATCC	1 cut(s)	286
BisI	GCNGC	4 cut(s)	8, 11, 87, 309
BlsI	GCNGC	4 cut(s)	9, 12, 88, 310
Bme1390I	CCNGG	5 cut(s)	91, 149, 219, 417, 872
Bme18I	GGWCC	1 cut(s)	101
BmgT120I	GGNCC	3 cut(s)	101, 215, 372
BmrFI	CCNGG	5 cut(s)	91, 149, 219, 417, 872
BmrI	ACTGGG	1 cut(s)	257
BmsI	GCATC	3 cut(s)	484, 506, 783
BmuI	ACTGGG	1 cut(s)	257
Bpu14I	TTCGAA	1 cut(s)	822
BpuMI	CCSGG	1 cut(s)	91
BsaBI	GATNNNNATC	1 cut(s)	753
BsaJI	CCNNGG	1 cut(s)	147
BsaXI	ACNNNNNCTCC	2 cut(s)	473, 503
Bsc4I	CCNNNNNNNGG	3 cut(s)	85, 212, 738
Bse1I	ACTGG	2 cut(s)	115, 263
Bse8I	GATNNNNATC	1 cut(s)	753
BseBI	CCWGG	4 cut(s)	149, 219, 417, 872
BseDI	CCNNGG	1 cut(s)	147
BseGI	GGATG	1 cut(s)	60
BseJI	GATNNNNATC	1 cut(s)	753
BseLI	CCNNNNNNNGG	3 cut(s)	85, 212, 738
BseMII	CTCAG	3 cut(s)	435, 555, 822
BseNI	ACTGG	2 cut(s)	115, 263
BseX3I	CGGCCG	1 cut(s)	87
BseXI	GCAGC	2 cut(s)	19, 295
Bsh1285I	CGRYCG	1 cut(s)	90
BshFI	GGCC	4 cut(s)	89, 217, 374, 393
BsiEI	CGRYCG	1 cut(s)	90
BsiHKAI	GWGCWC	2 cut(s)	336, 542
BsiSI	CCGG	2 cut(s)	90, 394
BslI	CCNNNNNNNGG	3 cut(s)	85, 212, 738
BsmAI	GTCTC	2 cut(s)	261, 377
BsnI	GGCC	4 cut(s)	89, 217, 374, 393
Bsp119I	TTCGAA	1 cut(s)	822
Bsp1286I	GDGCHC	2 cut(s)	336, 542
Bsp143I	GATC	3 cut(s)	160, 200, 396
BspACI	CCGC	5 cut(s)	53, 86, 213, 589, 761
BspANI	GGCC	4 cut(s)	89, 217, 374, 393
BspCNI	CTCAG	3 cut(s)	434, 554, 823
BspHI	TCATGA	1 cut(s)	558
BspPI	GGATC	2 cut(s)	208, 404
BspT104I	TTCGAA	1 cut(s)	822
BspTI	CTTAAG	1 cut(s)	131
BsrI	ACTGG	2 cut(s)	115, 263
BssECI	CCNNGG	1 cut(s)	147
BssMI	GATC	3 cut(s)	160, 200, 396
BssSI	CACGAG	1 cut(s)	176
Bst2BI	CACGAG	1 cut(s)	176
Bst2UI	CCWGG	4 cut(s)	149, 219, 417, 872
Bst4CI	ACNGT	4 cut(s)	101, 611, 772, 847
BstAFI	CTTAAG	1 cut(s)	131
BstBI	TTCGAA	1 cut(s)	822
BstC8I	GCNNGC	3 cut(s)	123, 215, 708
BstDEI	CTNAG	6 cut(s)	74, 421, 438, 541, 745, 831
BstF5I	GGATG	1 cut(s)	60
BstKTI	GATC	3 cut(s)	163, 203, 399
BstMAI	GTCTC	2 cut(s)	261, 377
BstMBI	GATC	3 cut(s)	160, 200, 396
BstMCI	CGRYCG	1 cut(s)	90
BstMWI	GCNNNNNNNGC	1 cut(s)	305
BstNI	CCWGG	4 cut(s)	149, 219, 417, 872
BstSCI	CCNGG	5 cut(s)	89, 147, 217, 415, 870
BstSFI	CTRYAG	1 cut(s)	846
BstV1I	GCAGC	2 cut(s)	19, 295
BstZI	CGGCCG	1 cut(s)	87
BsuI	GTATCC	1 cut(s)	286
BsuRI	GGCC	4 cut(s)	89, 217, 374, 393
BtsCI	GGATG	1 cut(s)	60
Cac8I	GCNNGC	3 cut(s)	123, 215, 708
CciI	TCATGA	1 cut(s)	558
Cfr13I	GGNCC	3 cut(s)	101, 215, 372
Csp6I	GTAC	2 cut(s)	46, 868
CviAII	CATG	4 cut(s)	369, 559, 650, 670
CviQI	GTAC	2 cut(s)	46, 868
DdeI	CTNAG	6 cut(s)	74, 421, 438, 541, 745, 831
DpnI	GATC	3 cut(s)	162, 202, 398
DpnII	GATC	3 cut(s)	160, 200, 396
DraI	TTTAAA	1 cut(s)	469
EaeI	YGGCCR	2 cut(s)	87, 391
EagI	CGGCCG	1 cut(s)	87
Ecl136II	GAGCTC	1 cut(s)	540
EclXI	CGGCCG	1 cut(s)	87
Eco24I	GRGCYC	1 cut(s)	542
Eco47I	GGWCC	1 cut(s)	101
Eco52I	CGGCCG	1 cut(s)	87
Eco53kI	GAGCTC	1 cut(s)	540
Eco57I	CTGAAG	1 cut(s)	42
EcoICRI	GAGCTC	1 cut(s)	540
EcoRII	CCWGG	4 cut(s)	147, 217, 415, 870
EcoT22I	ATGCAT	2 cut(s)	460, 499
EcoT38I	GRGCYC	1 cut(s)	542
FaeI	CATG	4 cut(s)	372, 562, 653, 673
FalI	AAGNNNNNCTT	2 cut(s)	296, 328
FatI	CATG	4 cut(s)	368, 558, 649, 669
FauI	CCCGC	1 cut(s)	206
Fnu4HI	GCNGC	4 cut(s)	8, 11, 87, 309
FokI	GGATG	1 cut(s)	47
FriOI	GRGCYC	1 cut(s)	542
Fsp4HI	GCNGC	4 cut(s)	8, 11, 87, 309
FspBI	CTAG	1 cut(s)	164
GluI	GCNGC	4 cut(s)	8, 11, 87, 309
HaeIII	GGCC	4 cut(s)	89, 217, 374, 393
HapII	CCGG	2 cut(s)	90, 394
Hin1II	CATG	4 cut(s)	372, 562, 653, 673
HincII	GTYRAC	3 cut(s)	427, 647, 775
HindII	GTYRAC	3 cut(s)	427, 647, 775
HindIII	AAGCTT	1 cut(s)	825
HinfI	GANTC	2 cut(s)	550, 734
HpaI	GTTAAC	2 cut(s)	427, 775
HpaII	CCGG	2 cut(s)	90, 394
HphI	GGTGA	4 cut(s)	42, 195, 223, 665
Hpy166II	GTNNAC	6 cut(s)	46, 144, 364, 427, 647, 775
Hpy188I	TCNGA	4 cut(s)	97, 733, 832, 840
Hpy188III	TCNNGA	3 cut(s)	501, 559, 622
Hpy8I	GTNNAC	6 cut(s)	46, 144, 364, 427, 647, 775
HpyAV	CCTTC	8 cut(s)	17, 136, 191, 282, 373, 455, 536, 579
HpyCH4III	ACNGT	4 cut(s)	101, 611, 772, 847
HpyCH4IV	ACGT	1 cut(s)	777
HpyCH4V	TGCA	2 cut(s)	458, 497
HpyF10VI	GCNNNNNNNGC	1 cut(s)	305
HpyF3I	CTNAG	6 cut(s)	74, 421, 438, 541, 745, 831
HpySE526I	ACGT	1 cut(s)	777
Hsp92II	CATG	4 cut(s)	372, 562, 653, 673
KspAI	GTTAAC	2 cut(s)	427, 775
Kzo9I	GATC	3 cut(s)	160, 200, 396
LmnI	GCTCC	3 cut(s)	418, 447, 537
Lsp1109I	GCAGC	2 cut(s)	19, 295
LweI	GCATC	3 cut(s)	484, 506, 783
MaeI	CTAG	1 cut(s)	164
MaeII	ACGT	1 cut(s)	777
MalI	GATC	3 cut(s)	162, 202, 398
MboI	GATC	3 cut(s)	160, 200, 396
MboII	GAAGA	4 cut(s)	674, 720, 749, 804
MhlI	GDGCHC	2 cut(s)	336, 542
MluCI	AATT	1 cut(s)	818
MlyI	GAGTC	2 cut(s)	544, 743
MnlI	CCTC	5 cut(s)	178, 185, 192, 475, 868
Mph1103I	ATGCAT	2 cut(s)	460, 499
MseI	TTAA	6 cut(s)	132, 314, 426, 468, 546, 774
MslI	CAYNNNNRTG	2 cut(s)	176, 674
MspA1I	CMGCKG	2 cut(s)	10, 55
MspCI	CTTAAG	1 cut(s)	131
MspI	CCGG	2 cut(s)	90, 394
MspR9I	CCNGG	5 cut(s)	91, 149, 219, 417, 872
MvaI	CCWGG	4 cut(s)	149, 219, 417, 872
MwoI	GCNNNNNNNGC	1 cut(s)	305
NciI	CCSGG	1 cut(s)	91
NdeII	GATC	3 cut(s)	160, 200, 396
NlaIII	CATG	4 cut(s)	372, 562, 653, 673
NmeAIII	GCCGAG	1 cut(s)	400
NsiI	ATGCAT	2 cut(s)	460, 499
NspV	TTCGAA	1 cut(s)	822
OliI	CACNNNNGTG	1 cut(s)	176
PagI	TCATGA	1 cut(s)	558
PkrI	GCNGC	4 cut(s)	9, 12, 88, 310
PleI	GAGTC	2 cut(s)	544, 742
PpsI	GAGTC	2 cut(s)	544, 742
Psp124BI	GAGCTC	1 cut(s)	542
Psp1406I	AACGTT	1 cut(s)	777
Psp6I	CCWGG	4 cut(s)	147, 217, 415, 870
PspGI	CCWGG	4 cut(s)	147, 217, 415, 870
PspPI	GGNCC	3 cut(s)	101, 215, 372
PvuII	CAGCTG	1 cut(s)	10
RsaI	GTAC	2 cut(s)	47, 869
RsaNI	GTAC	2 cut(s)	46, 868
RseI	CAYNNNNRTG	2 cut(s)	176, 674
SacI	GAGCTC	1 cut(s)	542
SaqAI	TTAA	6 cut(s)	132, 314, 426, 468, 546, 774
SatI	GCNGC	4 cut(s)	8, 11, 87, 309
Sau3AI	GATC	3 cut(s)	160, 200, 396
Sau96I	GGNCC	3 cut(s)	101, 215, 372
SchI	GAGTC	2 cut(s)	544, 743
ScrFI	CCNGG	5 cut(s)	91, 149, 219, 417, 872
SduI	GDGCHC	2 cut(s)	336, 542
SfaNI	GCATC	3 cut(s)	484, 506, 783
SfcI	CTRYAG	1 cut(s)	846
SfuI	TTCGAA	1 cut(s)	822
SinI	GGWCC	1 cut(s)	101
SmiMI	CAYNNNNRTG	2 cut(s)	176, 674
SmlI	CTYRAG	1 cut(s)	131
SmoI	CTYRAG	1 cut(s)	131
Sse9I	AATT	1 cut(s)	818
SsiI	CCGC	5 cut(s)	53, 86, 213, 589, 761
SspMI	CTAG	1 cut(s)	164
SstI	GAGCTC	1 cut(s)	542
StyD4I	CCNGG	5 cut(s)	89, 147, 217, 415, 870
TaaI	ACNGT	4 cut(s)	101, 611, 772, 847
TaiI	ACGT	1 cut(s)	780
TaqI	TCGA	3 cut(s)	17, 330, 822
TasI	AATT	1 cut(s)	818
TatI	WGTACW	1 cut(s)	45
TauI	GCSGC	1 cut(s)	89
Tru1I	TTAA	6 cut(s)	132, 314, 426, 468, 546, 774
Tru9I	TTAA	6 cut(s)	132, 314, 426, 468, 546, 774
TseI	GCWGC	3 cut(s)	7, 10, 308
TspDTI	ATGAA	3 cut(s)	17, 360, 547
Vha464I	CTTAAG	1 cut(s)	131
VpaK11BI	GGWCC	1 cut(s)	101
XapI	RAATTY	1 cut(s)	818
XcmI	CCANNNNNNNNNTGG	1 cut(s)	530
XspI	CTAG	1 cut(s)	164
Zsp2I	ATGCAT	2 cut(s)	460, 499

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.