Rmu_ssc0000422.1_g000010

Belongs to the serpin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000422.1
OG0000163
Physical Location & Seq
Forward (+)
25696 .. 33340
7645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000422.1_g000010.1.cds

Sequence Viewer

Length: 3915 bp
atgtttggttctacgagcccttttgggcagtcatctaacagcccatttgggtcccaatcggtgtttgggcaaagcagcaacacaagtaacaatccttttgctccccagccctttggtagcaccactccatttggtgcccagacagggagttctctaatttggggtacttcaactggcgtatttggtcaaacccaatcctccccttttggtgcttcatcctcgccagctttcggacagcacagttctccatttggagcatcttctactccagcatttggtgcaccatcatctactcaaacaagtccatttggtggagcatttgggtcgagtcctgctcaaacaagtccatttggaagcacagctcaaccatcacaaccagcatttggaagcaatatgtttggtaccacctctacagcatttggcgcaagtcagcctgcatttggcactaatactgccccagcctttggttcaacgaatgccacaggctttggtggccagagcacccctggttttggtggccaaggcactccagcctttggggccacaagcgcttcaccttttggtagtacaagtactccggcgtttggtgctccaagtactcctacctttggctcaacaccaagtcctacatttggaagcacaggatctgcatttgggacaacaagttccaacttgtttggatcagggggagcatttggggcttcaaccaccccagcttttggtcaatctagctcagcttttggtactacaacaagtgctcctgcttttggtcaatcaagttcaggttttggcttttcaacaagtgctcctgcatttggccaatcaagttcaacctttggtagcacccaatttgcggcttcatctcctttcggagcgcagagttcgccatctcctttcggagggcagagttcgccatttggggctcagtcaacaacaccaacacttggaaacactgcctttggacagccagcttttgggggccaacggggtggaagtagagtggctgcttacacaggcacatctgaaccagatagtaatggaatggggaaattggagtcaatatcagcaatgccagcctataaagaaaaaagtcacgaggaattgagatgggaggattaccaattaggggataagggtggaccgtctcctgctggtgcgggtagctttggtgtatcaactgttcagacaagtactttgaatcctaatcctgcaccatcgttttctcaaacatcttcaagtccttttaatacctcaacctcatctggtttatttgcccaaaaaccctcatcctttccttctactggttttggtgttacttcatcttcaccatttagttcaacttcaccgtttagttcttcatcaactcaaaccaatgtttttacacctgcatcatctgcgtcaccatttggccaaacgttgtctccagctatttttagttcaccgtcttccttttcattctctaccccggctacatcatccactccctcatttaattttcccactccatcaaacccacagacacagtttggtcaaacaggcactacctcctgtggttcaaacttgttcaataatacttccagtttgcagagcagttcattgggtactacaagcaaccacctgggtattactcaaccagctacatctcagcctcttcagactagtccttttggcttcagcaacaccaacacctttaatcaaccacagccaggcaacacaagcgcctttggtggtttagcaggcatttctggtcagagcaactttggacaatcgtctgtggctcaaggctctgcagctgtacaacaaccagtacctgctacaaatccatttggaacgctccctgcgatgcctcagatgtcaattgctccttatcagaagcctgattctggaactactacttcagtcacttctggcactagtatcaccgtaaacccaacttctggttctgctgtcactataacccccagtcctggcagtactgtcaccataaccatctcaatttctggtagtactactatcaatatatcccccacttctgaaactacctcaagtaatacaccttcgccaactggagaaacttcatctccatttagctttaggcttccccatcctgccccaaagtctccgagtttatctcaacccttcaacagtttggataaaaaaccaccagctgtacaaccaatacattctacaagctcgtctctggttccaactcctgttcctgcacagccaattgaagatggtgtcagagaatccattaaaaaccagaacgatgtcacactgggaatcacaaagcaattgcttctggatgtaggcagggacaacaacatggtgtactcgccgctgtccatccacatggttcttggcctgatagcagctggatcaacaagaggaaccaacagggaccagattctcaatttcctgaaggcaaagtccactgaggaactcaatgactttgcttccaagcttgtccctctggtctttgctgacggatccccaaagggagggccttgcttatcattctctaatagtgtttggctcgacaagtctctcagtttcaaggcttctttcaaacatgtagtggacaatgcatacaaggcggctttgaatcaagttgactttaagaccaaggcagaggaaatccgatgtgaagtgaattcatgggccgaggaggagactaaaggcctcatcaaagaggttcttccccctatgtcagttgacagcacaacaaggcttatccttgctaatgcattatactttaaaggagattggactgagaagttcgacgcatcacttacaaaaacagatgatttccaccttatcgatgggaggtcagtaaaggcacccttcatgaccagctggaataaacaattcataagtgcctttgacggtttcaaagtcttaaagcttccttacgaacaaggtggagatcacattcggcgtttctccatgtatgtgtatcttccaaatgcaatagatggcctgccagctttagttgagagagtttgttcagagtctggatttgtggatcgctatcttccccgctttgcagttaaagttggtagatttttgatcccgaagtttaagctctctgctggctttgaggttaaagaagttctgcaaactttaggactagacttgccacttcatcctggaagtttgtcacagatggtggactcatctttggggctatgtatgtcttgcatacgccataaatccttcattgaagttaatgaagaaggcacagaaaccgcgactgttactgttgcctttggtttatctgactgtatctcaatccgagatccgccgatagactttgtggcagatcacccattccttttcctgatcagagaagaaacatctggagcagtgctgttcattgcaagtgcacctggttttagttcttcaagtactccatctttcagctgtccccctcctgctgttggggaatcaaattctacatttggtaccgttccattaggtgcttcatctcctccattgcagagttttgcatttgagtctggatttgtggatcgctatcttccccgctttgcagttaaagttggtagatttttgatcccgaagtttaagctctctgctggctttgaggttaaagaagttctgcaaactttaggactagacttgccacttcatcctggaagtttgtcacagatggtggactcatctttggggctatgtatgtcttgcatacgccataaatccttcattgaagttaatgaagaaggcacagaaaccgcgactgttactgttgcctttggtttatctgactgtatctcaatccgagatccgccgatagactttgtggcagatcacccattccttttcctgatcagagaagaaacatctggagcagtgctgttcattggtcatgtcctcaatcccattgcagatcaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05146 ko05164 map03013 map05146 map05164
Pfam Domains
Protein Families
Serpin

Protein Analysis

1304

Amino Acids

134.97

Weight (kDa)

5.67

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64020
fragaria_vesca FvH4_1g24600 FvH4_1g24640 FvH4_2g15000 FvH4_3g03700 FvH4_3g03710 FvH4_3g09790 FvH4_3g09790 FvH4_3g09810 FvH4_3g09811 FvH4_3g23720 FvH4_3g32870 FvH4_3g32890 FvH4_3g37550 FvH4_3g37560 FvH4_5g01890 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g21100 FvH4_5g21110 FvH4_5g21330 FvH4_5g21331 FvH4_5g21520 FvH4_5g21540 FvH4_5g21970 FvH4_6g01280
malus_domestica MD00G1084800.v1.1 MD00G1141300.v1.1 MD02G1102800.v1.1 MD02G1282200.v1.1 MD02G1282300.v1.1 MD03G1207900.v1.1 MD03G1230000.v1.1 MD04G1136400.v1.1 MD04G1136500.v1.1 MD04G1189000.v1.1 MD05G1248700.v1.1 MD05G1248800.v1.1 MD06G1030400.v1.1 MD10G1229200.v1.1 MD10G1229300.v1.1 MD15G1291400.v1.1 MD15G1431300.v1.1
prunus_persica Prupe.5G041800_v2.0.a1 Prupe.5G041900_v2.0.a1 Prupe.5G042000_v2.0.a1 Prupe.5G042100_v2.0.a1 Prupe.5G042200_v2.0.a1
pyrus_communis pycom02g14630 pycom02g24090 pycom04g12410 pycom04g16760 pycom05g22550 pycom10g19280 pycom12g05670 pycom15g25490 pycom15g38110
rosa_chinensis RchiOBHm_Chr2g0122001 RchiOBHm_Chr2g0163171 RchiOBHm_Chr2g0163181 RchiOBHm_Chr3g0455201 RchiOBHm_Chr5g0005751 RchiOBHm_Chr5g0015501 RchiOBHm_Chr5g0015511 RchiOBHm_Chr5g0015521 RchiOBHm_Chr5g0015531 RchiOBHm_Chr5g0060021 RchiOBHm_Chr7g0187971 RchiOBHm_Chr7g0207951 RchiOBHm_Chr7g0208671 RchiOBHm_Chr7g0208691 RchiOBHm_Chr7g0208941 RchiOBHm_Chr7g0208951 RchiOBHm_Chr7g0208961
rosa_laevigata RLG00000003132 RLG00000003177 RLG00000003178 RLG00000003179 RLG00000003180 RLG00000003201 RLG00000003202 RLG00000003270 RLG00000004739 RLG00000004741 RLG00000004744 RLG00000021379 RLG00000025378 RLG00000031323 RLG00000031324 RLG00000032205 RLG00000032206 RLG00000035339
rosa_multiflora Rmu_co8189380.1_g000001 Rmu_co8284591.1_g000001 Rmu_co8360283.1_g000002 Rmu_co8379053.1_g000001 Rmu_sc0000493.1_g000007 Rmu_sc0000532.1_g000030 Rmu_sc0000532.1_g000031 Rmu_sc0000570.1_g000033 Rmu_sc0000905.1_g000012 Rmu_sc0001730.1_g000002 Rmu_sc0002045.1_g000039 Rmu_sc0003064.1_g000001 Rmu_sc0003064.1_g000002 Rmu_sc0003064.1_g000006 Rmu_sc0003880.1_g000012 Rmu_sc0004103.1_g000003 Rmu_sc0005082.1_g000007 Rmu_sc0011272.1_g000002 Rmu_sc0015523.1_g000021 Rmu_sc0015523.1_g000022 Rmu_sc0019960.1_g000007 Rmu_sc0029514.1_g000001 Rmu_ssc0000422.1_g000010
rosa_roxburghii Rroxscaffold_1G00013660 Rroxscaffold_1G00060980 Rroxscaffold_1G00060990 Rroxscaffold_1G00061000 Rroxscaffold_1G00061010 Rroxscaffold_2G00087490 Rroxscaffold_2G00122080 Rroxscaffold_2G00123490 Rroxscaffold_3G00249930 Rroxscaffold_3G00249940 Rroxscaffold_3G00249950 Rroxscaffold_3G00249980 Rroxscaffold_3G00250310 Rroxscaffold_3G00250320 Rroxscaffold_3G00266850 Rroxscaffold_3G00266870 Rroxscaffold_3G00266910 Rroxscaffold_6G00424480
rosa_rugosa Rorug02G0228900 Rorug02G0236000 Rorug02G0501100 Rorug03G0004700 Rorug03G0004700 Rorug03G0004800 Rorug04G0417800 Rorug05G0027800 Rorug05G0027900 Rorug05G0028000 Rorug05G0327700 Rorug05G0384800 Rorug06G0486000 Rorug06G0486100 Rorug06G0486200 Rorug06G0486200 Rorug06G0486300 Rorug06G0486400 Rorug06G0486500 Rorug07G0100400 Rorug07G0105400 Rorug07G0105400 Rorug07G0107300 Rorug07G0107400 Rorug07G0107500 Rorug07G0113300
rosa_samantha Rh7AG091300 Rh7BG092800 Rh7CG091600 Rh7CG257500 Rh7DG093500
rosa_wichuraiana Rw0G001260 Rw0G017680 Rw2G023470 Rw3G005130 Rw5G004560 Rw5G010480 Rw7G020400 Rw7G020410 Rw7G020540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1393
Acc36I ACCTGC 2 cut(s) 1393, 1819
Acc65I GGTACC 2 cut(s) 401, 3464
AccB1I GGYRCC 4 cut(s) 134, 401, 2839, 3464
AccB7I CCANNNNNTGG 9 cut(s) 275, 311, 383, 464, 536, 719, 944, 974, 1937
AccII CGCG 2 cut(s) 3251, 3755
AclI AACGTT 1 cut(s) 1415
AcoI YGGCCR 4 cut(s) 493, 517, 817, 1408
AcsI RAATTY 2 cut(s) 2653, 3451
AcuI CTGAAG 4 cut(s) 1637, 1657, 1881, 2441
AfeI AGCGCT 1 cut(s) 550
AflIII ACRYGT 1 cut(s) 2572
AhlI ACTAGT 2 cut(s) 1658, 1913
AjnI CCWGG 7 cut(s) 505, 1617, 1705, 1966, 3148, 3388, 3652
AjuI GAANNNNNNNTTGG 2 cut(s) 1924, 1956
AloI GAACNNNNNNTCC 2 cut(s) 649, 681
Alw21I GWGCWC 6 cut(s) 283, 503, 592, 760, 808, 3388
Alw26I GTCTC 6 cut(s) 1149, 1425, 2124, 2202, 2550, 2666
Alw44I GTGCAC 2 cut(s) 279, 3384
AlwNI CAGNNNCTG 3 cut(s) 647, 1811, 3014
Aor51HI AGCGCT 1 cut(s) 550
ApaLI GTGCAC 2 cut(s) 279, 3384
ApeKI GCWGC 4 cut(s) 75, 1004, 1790, 2372
ApoI RAATTY 2 cut(s) 2653, 3451
ArsI GACNNNNNNTTYG 2 cut(s) 1177, 1209
Asp718I GGTACC 2 cut(s) 401, 3464
AspLEI GCGC 4 cut(s) 425, 551, 877, 1721
AspS9I GGNCC 7 cut(s) 51, 540, 979, 1139, 2401, 2504, 2661
AsuC2I CCSGG 1 cut(s) 1466
AsuHPI GGTGA 9 cut(s) 546, 1317, 1335, 1392, 1431, 1912, 1972, 3317, 3821
AvaII GGWCC 3 cut(s) 51, 1139, 2401
BaeGI GKGCMC 3 cut(s) 139, 283, 3388
BalI TGGCCA 4 cut(s) 495, 519, 819, 1410
BamHI GGATCC 1 cut(s) 2489
BanI GGYRCC 4 cut(s) 134, 401, 2839, 3464
BanII GRGCYC 2 cut(s) 20, 925
BarI GAAGNNNNNNTAC 2 cut(s) 2041, 2073
BauI CACGAG 1 cut(s) 1094
BbsI GAAGAC 1 cut(s) 1437
Bbv12I GWGCWC 6 cut(s) 283, 503, 592, 760, 808, 3388
BbvI GCAGC 4 cut(s) 87, 991, 1802, 2384
BcgI CGANNNNNNTGC 2 cut(s) 306, 340
BciT130I CCWGG 7 cut(s) 507, 1619, 1707, 1968, 3150, 3390, 3654
BclI TGATCA 2 cut(s) 3342, 3846
BcnI CCSGG 1 cut(s) 1466
BcoDI GTCTC 6 cut(s) 1149, 1425, 2124, 2202, 2550, 2666
BcuI ACTAGT 2 cut(s) 1658, 1913
BfaI CTAG 5 cut(s) 729, 1659, 1914, 3131, 3635
BfmI CTRYAG 2 cut(s) 411, 1788
BfoI RGCGCY 2 cut(s) 552, 1722
BfuAI ACCTGC 2 cut(s) 1393, 1819
BglI GCCNNNNNGGC 1 cut(s) 539
BisI GCNGC 7 cut(s) 76, 855, 1005, 1791, 2339, 2373, 2598
BlpI GCTNAGC 1 cut(s) 733
BlsI GCNGC 7 cut(s) 77, 856, 1006, 1792, 2340, 2374, 2599
BmcAI AGTACT 6 cut(s) 574, 598, 1192, 1975, 2008, 3409
Bme1390I CCNGG 8 cut(s) 507, 1466, 1619, 1707, 1968, 3150, 3390, 3654
Bme18I GGWCC 3 cut(s) 51, 1139, 2401
BmgT120I GGNCC 7 cut(s) 51, 540, 979, 1139, 2401, 2504, 2661
BmrFI CCNGG 8 cut(s) 507, 1466, 1619, 1707, 1968, 3150, 3390, 3654
BmrI ACTGGG 2 cut(s) 1956, 2288
BmsI GCATC 4 cut(s) 266, 1397, 1833, 2795
BmuI ACTGGG 2 cut(s) 1956, 2288
BoxI GACNNNNGTC 1 cut(s) 1768
BpiI GAAGAC 1 cut(s) 1437
BplI GAGNNNNNCTC 4 cut(s) 319, 351, 2116, 2148
BpmI CTGGAG 6 cut(s) 252, 513, 1407, 2088, 3381, 3885
Bpu1102I GCTNAGC 1 cut(s) 733
BpuEI CTTGAG 2 cut(s) 1764, 2029
BpuMI CCSGG 1 cut(s) 1466
Bsa29I ATCGAT 1 cut(s) 2820
BsaBI GATNNNNATC 2 cut(s) 3030, 3534
BsaJI CCNNGG 6 cut(s) 505, 520, 1464, 1618, 2625, 2664
Bse1I ACTGG 7 cut(s) 178, 1306, 1578, 1805, 1962, 2071, 2283
Bse3DI GCAATG 4 cut(s) 1074, 3375, 3494, 3900
Bse8I GATNNNNATC 2 cut(s) 3030, 3534
BseBI CCWGG 7 cut(s) 507, 1619, 1707, 1968, 3150, 3390, 3654
BseCI ATCGAT 1 cut(s) 2820
BseDI CCNNGG 6 cut(s) 505, 520, 1464, 1618, 2625, 2664
BseGI GGATG 8 cut(s) 215, 1286, 1475, 2104, 2311, 2346, 3145, 3649
BseJI GATNNNNATC 2 cut(s) 3030, 3534
BseMI GCAATG 4 cut(s) 1074, 3375, 3494, 3900
BseMII CTCAG 7 cut(s) 747, 938, 1658, 1862, 2427, 2563, 2763
BseNI ACTGG 7 cut(s) 178, 1306, 1578, 1805, 1962, 2071, 2283
BseRI GAGGAG 3 cut(s) 2681, 2684, 3480
BseSI GKGCMC 3 cut(s) 139, 283, 3388
BseXI GCAGC 4 cut(s) 87, 991, 1802, 2384
BseYI CCCAGC 3 cut(s) 105, 457, 712
BsgI GTGCAG 2 cut(s) 1194, 2205
Bsh1236I CGCG 2 cut(s) 3251, 3755
BshNI GGYRCC 4 cut(s) 134, 401, 2839, 3464
BshVI ATCGAT 1 cut(s) 2820
BsiHKAI GWGCWC 6 cut(s) 283, 503, 592, 760, 808, 3388
BsiSI CCGG 2 cut(s) 578, 1466
BslFI GGGAC 6 cut(s) 37, 670, 2330, 2414, 2453, 3411
BsmAI GTCTC 6 cut(s) 1149, 1425, 2124, 2202, 2550, 2666
BsmBI CGTCTC 2 cut(s) 1149, 2202
BsmFI GGGAC 6 cut(s) 37, 670, 2330, 2414, 2453, 3411
BsmI GAATGC 1 cut(s) 481
Bsp1286I GDGCHC 9 cut(s) 20, 139, 283, 503, 592, 760, 808, 925, 3388
Bsp1407I TGTACA 2 cut(s) 1795, 2170
Bsp1720I GCTNAGC 1 cut(s) 733
BspCNI CTCAG 7 cut(s) 746, 937, 1657, 1861, 2428, 2562, 2764
BspDI ATCGAT 1 cut(s) 2820
BspFNI CGCG 2 cut(s) 3251, 3755
BspHI TCATGA 1 cut(s) 2847
BspMAI CTGCAG 1 cut(s) 1792
BspMI ACCTGC 2 cut(s) 1393, 1819
BspT107I GGYRCC 4 cut(s) 134, 401, 2839, 3464
BsrDI GCAATG 4 cut(s) 1074, 3375, 3494, 3900
BsrGI TGTACA 2 cut(s) 1795, 2170
BsrI ACTGG 7 cut(s) 178, 1306, 1578, 1805, 1962, 2071, 2283
BssECI CCNNGG 6 cut(s) 505, 520, 1464, 1618, 2625, 2664
BssSI CACGAG 1 cut(s) 1094
BssT1I CCWWGG 2 cut(s) 520, 2625
Bst2BI CACGAG 1 cut(s) 1094
Bst2UI CCWGG 7 cut(s) 507, 1619, 1707, 1968, 3150, 3390, 3654
Bst6I CTCTTC 1 cut(s) 1656
BstAPI GCANNNNNTGC 2 cut(s) 278, 1394
BstAUI TGTACA 2 cut(s) 1795, 2170
BstC8I GCNNGC 9 cut(s) 225, 435, 969, 1074, 1738, 2981, 2985, 3094, 3598
BstDEI CTNAG 7 cut(s) 733, 924, 1644, 1848, 2436, 2549, 2772
BstENI CCTNNNNNAGG 1 cut(s) 897
BstF5I GGATG 8 cut(s) 215, 1286, 1475, 2104, 2311, 2346, 3145, 3649
BstFNI CGCG 2 cut(s) 3251, 3755
BstH2I RGCGCY 2 cut(s) 552, 1722
BstHHI GCGC 4 cut(s) 425, 551, 877, 1721
BstMAI GTCTC 6 cut(s) 1149, 1425, 2124, 2202, 2550, 2666
BstNI CCWGG 7 cut(s) 507, 1619, 1707, 1968, 3150, 3390, 3654
BstNSI RCATGY 1 cut(s) 2576
BstPAI GACNNNNGTC 1 cut(s) 1768
BstSCI CCNGG 8 cut(s) 505, 1464, 1617, 1705, 1966, 3148, 3388, 3652
BstSFI CTRYAG 2 cut(s) 411, 1788
BstSLI GKGCMC 3 cut(s) 139, 283, 3388
BstUI CGCG 2 cut(s) 3251, 3755
BstV1I GCAGC 4 cut(s) 87, 991, 1802, 2384
BstV2I GAAGAC 1 cut(s) 1437
BstX2I RGATCY 4 cut(s) 644, 2489, 3298, 3802
BstXI CCANNNNNNTGG 3 cut(s) 113, 989, 2353
BstYI RGATCY 4 cut(s) 644, 2489, 3298, 3802
Bsu15I ATCGAT 1 cut(s) 2820
BsuTUI ATCGAT 1 cut(s) 2820
BtgZI GCGATG 1 cut(s) 1856
BtsCI GGATG 8 cut(s) 215, 1286, 1475, 2104, 2311, 2346, 3145, 3649
BtsI GCAGTG 3 cut(s) 951, 3372, 3876
BtsIMutI CAGTG 5 cut(s) 951, 2276, 2433, 3372, 3876
BveI ACCTGC 2 cut(s) 1393, 1819
Cac8I GCNNGC 9 cut(s) 225, 435, 969, 1074, 1738, 2981, 2985, 3094, 3598
CaiI CAGNNNCTG 3 cut(s) 647, 1811, 3014
CciI TCATGA 1 cut(s) 2847
CfoI GCGC 4 cut(s) 425, 551, 877, 1721
Cfr13I GGNCC 7 cut(s) 51, 540, 979, 1139, 2401, 2504, 2661
ClaI ATCGAT 1 cut(s) 2820
CseI GACGC 2 cut(s) 1386, 2792
CsiI ACCWGGT 1 cut(s) 3388
CspCI CAANNNNNGTGG 4 cut(s) 112, 147, 469, 504
CviAII CATG 7 cut(s) 2326, 2353, 2573, 2658, 2848, 2947, 3887
DdeI CTNAG 7 cut(s) 733, 924, 1644, 1848, 2436, 2549, 2772
DraI TTTAAA 1 cut(s) 2758
EaeI YGGCCR 4 cut(s) 493, 517, 817, 1408
Eam1104I CTCTTC 1 cut(s) 1656
EarI CTCTTC 1 cut(s) 1656
EciI GGCGGA 2 cut(s) 3291, 3795
Eco130I CCWWGG 2 cut(s) 520, 2625
Eco147I AGGCCT 1 cut(s) 2682
Eco24I GRGCYC 2 cut(s) 20, 925
Eco47I GGWCC 3 cut(s) 51, 1139, 2401
Eco47III AGCGCT 1 cut(s) 550
Eco57I CTGAAG 4 cut(s) 1637, 1657, 1881, 2441
EcoNI CCTNNNNNAGG 1 cut(s) 897
EcoO109I RGGNCCY 2 cut(s) 51, 2504
EcoRI GAATTC 1 cut(s) 2653
EcoRII CCWGG 7 cut(s) 505, 1617, 1705, 1966, 3148, 3388, 3652
EcoT14I CCWWGG 2 cut(s) 520, 2625
EcoT22I ATGCAT 2 cut(s) 2590, 2749
EcoT38I GRGCYC 2 cut(s) 20, 925
ErhI CCWWGG 2 cut(s) 520, 2625
Esp3I CGTCTC 2 cut(s) 1149, 2202
FaeI CATG 7 cut(s) 2329, 2356, 2576, 2661, 2851, 2950, 3890
FalI AAGNNNNNCTT 4 cut(s) 2682, 2714, 2828, 2860
FaqI GGGAC 6 cut(s) 37, 670, 2330, 2414, 2453, 3411
FatI CATG 7 cut(s) 2325, 2352, 2572, 2657, 2847, 2946, 3886
FauI CCCGC 3 cut(s) 1150, 3047, 3551
FbaI TGATCA 2 cut(s) 3342, 3846
Fnu4HI GCNGC 7 cut(s) 76, 855, 1005, 1791, 2339, 2373, 2598
FokI GGATG 8 cut(s) 202, 1273, 1462, 2091, 2318, 2333, 3132, 3636
FriOI GRGCYC 2 cut(s) 20, 925
Fsp4HI GCNGC 7 cut(s) 76, 855, 1005, 1791, 2339, 2373, 2598
FspBI CTAG 5 cut(s) 729, 1659, 1914, 3131, 3635
GlaI GCGC 4 cut(s) 424, 550, 876, 1720
GluI GCNGC 7 cut(s) 76, 855, 1005, 1791, 2339, 2373, 2598
GsaI CCCAGC 3 cut(s) 109, 461, 716
GsuI CTGGAG 6 cut(s) 252, 513, 1407, 2088, 3381, 3885
HaeII RGCGCY 2 cut(s) 552, 1722
HapII CCGG 2 cut(s) 578, 1466
HgaI GACGC 2 cut(s) 1386, 2792
HhaI GCGC 4 cut(s) 425, 551, 877, 1721
Hin1II CATG 7 cut(s) 2329, 2356, 2576, 2661, 2851, 2950, 3890
Hin6I GCGC 4 cut(s) 423, 549, 875, 1719
HinP1I GCGC 4 cut(s) 423, 549, 875, 1719
HincII GTYRAC 3 cut(s) 930, 2614, 2716
HindII GTYRAC 3 cut(s) 930, 2614, 2716
HindIII AAGCTT 2 cut(s) 2462, 2903
HpaII CCGG 2 cut(s) 578, 1466
HphI GGTGA 9 cut(s) 546, 1317, 1335, 1392, 1431, 1912, 1972, 3317, 3821
Hpy99I CGWCG 1 cut(s) 2786
HpyAV CCTTC 9 cut(s) 1305, 2067, 2149, 2416, 2854, 3226, 3230, 3730, 3734
HpyCH4IV ACGT 1 cut(s) 1415
HpyF3I CTNAG 7 cut(s) 733, 924, 1644, 1848, 2436, 2549, 2772
HpySE526I ACGT 1 cut(s) 1415
Hsp92II CATG 7 cut(s) 2329, 2356, 2576, 2661, 2851, 2950, 3890
HspAI GCGC 4 cut(s) 423, 549, 875, 1719
KflI GGGWCCC 1 cut(s) 51
KpnI GGTACC 2 cut(s) 405, 3468
Ksp22I TGATCA 2 cut(s) 3342, 3846
Lsp1109I GCAGC 4 cut(s) 87, 991, 1802, 2384
LweI GCATC 4 cut(s) 266, 1397, 1833, 2795
MabI ACCWGGT 1 cut(s) 3388
MaeI CTAG 5 cut(s) 729, 1659, 1914, 3131, 3635
MaeII ACGT 1 cut(s) 1415
MfeI CAATTG 3 cut(s) 1857, 2229, 2294
MflI RGATCY 4 cut(s) 644, 2489, 3298, 3802
MhlI GDGCHC 9 cut(s) 20, 139, 283, 503, 592, 760, 808, 925, 3388
MlsI TGGCCA 4 cut(s) 495, 519, 819, 1410
MluNI TGGCCA 4 cut(s) 495, 519, 819, 1410
MlyI GAGTC 6 cut(s) 337, 1064, 3020, 3167, 3524, 3671
MmeI TCCRAC 2 cut(s) 693, 2231
Mox20I TGGCCA 4 cut(s) 495, 519, 819, 1410
Mph1103I ATGCAT 2 cut(s) 2590, 2749
MscI TGGCCA 4 cut(s) 495, 519, 819, 1410
MslI CAYNNNNRTG 2 cut(s) 2351, 2951
Msp20I TGGCCA 4 cut(s) 495, 519, 819, 1410
MspA1I CMGCKG 6 cut(s) 1793, 2168, 2341, 2375, 2856, 3423
MspI CCGG 2 cut(s) 578, 1466
MspR9I CCNGG 8 cut(s) 507, 1466, 1619, 1707, 1968, 3150, 3390, 3654
MunI CAATTG 3 cut(s) 1857, 2229, 2294
Mva1269I GAATGC 1 cut(s) 481
MvaI CCWGG 7 cut(s) 507, 1619, 1707, 1968, 3150, 3390, 3654
MvnI CGCG 2 cut(s) 3251, 3755
NciI CCSGG 1 cut(s) 1466
NlaIII CATG 7 cut(s) 2329, 2356, 2576, 2661, 2851, 2950, 3890
NmeAIII GCCGAG 1 cut(s) 2689
NmuCI GTSAC 8 cut(s) 1091, 1398, 1900, 1948, 1978, 2272, 3159, 3663
NsiI ATGCAT 2 cut(s) 2590, 2749
NspI RCATGY 1 cut(s) 2576
PagI TCATGA 1 cut(s) 2847
PaqCI CACCTGC 1 cut(s) 1393
PceI AGGCCT 1 cut(s) 2682
PciI ACATGT 1 cut(s) 2572
PcsI WCGNNNNNNNCGW 1 cut(s) 1838
PctI GAATGC 1 cut(s) 481
PfeI GAWTC 7 cut(s) 1198, 1880, 2249, 2283, 2407, 2605, 3446
PflMI CCANNNNNTGG 9 cut(s) 275, 311, 383, 464, 536, 719, 944, 974, 1937
PfoI TCCNGGA 2 cut(s) 3148, 3652
PkrI GCNGC 7 cut(s) 77, 856, 1006, 1792, 2340, 2374, 2599
PleI GAGTC 6 cut(s) 336, 1063, 3019, 3167, 3523, 3671
PpsI GAGTC 6 cut(s) 336, 1063, 3019, 3167, 3523, 3671
PpuMI RGGWCCY 1 cut(s) 51
PscI ACATGT 1 cut(s) 2572
PshAI GACNNNNGTC 1 cut(s) 1768
Psp1406I AACGTT 1 cut(s) 1415
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 7 cut(s) 505, 1617, 1705, 1966, 3148, 3388, 3652
PspFI CCCAGC 3 cut(s) 105, 457, 712
PspGI CCWGG 7 cut(s) 505, 1617, 1705, 1966, 3148, 3388, 3652
PspPI GGNCC 7 cut(s) 51, 540, 979, 1139, 2401, 2504, 2661
PspPPI RGGWCCY 1 cut(s) 51
PsrI GAACNNNNNNTAC 2 cut(s) 1164, 1196
PstI CTGCAG 1 cut(s) 1792
PstNI CAGNNNCTG 3 cut(s) 647, 1811, 3014
PsuI RGATCY 4 cut(s) 644, 2489, 3298, 3802
PvuII CAGCTG 5 cut(s) 1793, 2168, 2375, 2856, 3423
RseI CAYNNNNRTG 2 cut(s) 2351, 2951
SatI GCNGC 7 cut(s) 76, 855, 1005, 1791, 2339, 2373, 2598
Sau96I GGNCC 7 cut(s) 51, 540, 979, 1139, 2401, 2504, 2661
ScaI AGTACT 6 cut(s) 574, 598, 1192, 1975, 2008, 3409
SchI GAGTC 6 cut(s) 337, 1064, 3020, 3167, 3524, 3671
ScrFI CCNGG 8 cut(s) 507, 1466, 1619, 1707, 1968, 3150, 3390, 3654
SduI GDGCHC 9 cut(s) 20, 139, 283, 503, 592, 760, 808, 925, 3388
SexAI ACCWGGT 1 cut(s) 3388
SfaNI GCATC 4 cut(s) 266, 1397, 1833, 2795
SfcI CTRYAG 2 cut(s) 411, 1788
SinI GGWCC 3 cut(s) 51, 1139, 2401
SmiMI CAYNNNNRTG 2 cut(s) 2351, 2951
SmlI CTYRAG 2 cut(s) 1779, 2044
SmoI CTYRAG 2 cut(s) 1779, 2044
SpeI ACTAGT 2 cut(s) 1658, 1913
SseBI AGGCCT 1 cut(s) 2682
SspMI CTAG 5 cut(s) 729, 1659, 1914, 3131, 3635
StuI AGGCCT 1 cut(s) 2682
StyD4I CCNGG 8 cut(s) 505, 1464, 1617, 1705, 1966, 3148, 3388, 3652
StyI CCWWGG 2 cut(s) 520, 2625
TaiI ACGT 1 cut(s) 1418
TaqI TCGA 4 cut(s) 326, 2538, 2781, 2820
TauI GCSGC 3 cut(s) 857, 2341, 2600
TfiI GAWTC 7 cut(s) 1198, 1880, 2249, 2283, 2407, 2605, 3446
TscAI CASTG 5 cut(s) 958, 2283, 2440, 3372, 3876
TseFI GTSAC 8 cut(s) 1091, 1398, 1900, 1948, 1978, 2272, 3159, 3663
TseI GCWGC 4 cut(s) 75, 1004, 1790, 2372
Tsp45I GTSAC 8 cut(s) 1091, 1398, 1900, 1948, 1978, 2272, 3159, 3663
TspGWI ACGGA 1 cut(s) 2502
TspRI CASTG 5 cut(s) 958, 2283, 2440, 3372, 3876
Van91I CCANNNNNTGG 9 cut(s) 275, 311, 383, 464, 536, 719, 944, 974, 1937
VneI GTGCAC 2 cut(s) 279, 3384
VpaK11BI GGWCC 3 cut(s) 51, 1139, 2401
XagI CCTNNNNNAGG 1 cut(s) 897
XapI RAATTY 2 cut(s) 2653, 3451
XceI RCATGY 1 cut(s) 2576
XcmI CCANNNNNNNNNTGG 4 cut(s) 62, 503, 2357, 2819
XspI CTAG 5 cut(s) 729, 1659, 1914, 3131, 3635
ZrmI AGTACT 6 cut(s) 574, 598, 1192, 1975, 2008, 3409
Zsp2I ATGCAT 2 cut(s) 2590, 2749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.