Rroxscaffold_3G00249950

Belongs to the serpin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000163
Physical Location & Seq
Reverse (-)
43707706 .. 43709617
1912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00249950.1

Sequence Viewer

Length: 1422 bp
ATGGCAGCAACCCACTCCCTCCTCGGCCGTTCACAGGCCCTCCGTCCAAATTCTTCCGGCCATCGTCTGCCGGTTTCCTTTCTCGTTCTCTTTCTCACTTTCGTCATTGTATGTATTTCTCTCTTTTGTCCTCTCTCTGATCTGCTTTACCAGAGCCCAAACTCCGATCCTAATACTTTCAACGCCGATGACCATATCAATCCGATCCCGTGGAGTTCAATGAACCAGACCCAGAAAGCCAGAGTTCAAACATACATCAGCAACCACACCGAGGTGGCTTTTACTATCATGAAGCAGCTGTTTTTGAGCACTGAAGCCAAGAACAAAAATATGGTGTACTCGCCGCTATCCATCCACATGGTTCTCAGCCTTTTAGCGGCCGGGTCAAACTGCCCTACACAGGACCAGTTGCTCACCTTCCTTAAGTCCAAGTCCACCCAAGAGCTCAACTATCTAGCCTCCACTCTCATGCCTCTGTTCTTCGCGAAAGAATCACCAAATGGCGGGCCTAGCTTGTCATTCACCAATGGCGCTTGGGTAGACATGTCTCTTCCTGTCAAGCCTTCTTTCAAACGGATAGTGGACACTGCTTACAGGGCTGCCTTAAAGCAAGTCGATTTCAAGGGCAAGTCGGATGAAGTTAGAATGGAAGTGAATTCATGGGCCGAGAAGGAGACTAATGGTCGCATCAAAGATATTCTTGCTCCTGGCGTAGTTGACAGCCAAACTAGGTTCATTCTCGCCAATGCATTATACTTCAAAGGAGATTGGGAAGACAGGTTTGATGCATCAGGAACGAAAGAGTATGATTTTCACCTTCACAATGGGGGCTCAGTCAAGATACCTTTCATGACAGAGTGGATAAGGAGCTGGGGGAGGCGGTATGTAAGTGTTTTTGAGGGTTTCAAAGTTTTGAAACTCCCTTACAAACAAGGCCAAGATCTGAAGAGGTTCTCCATGTATGTGTTTCTTCCGAATGAAAGAGATGGGTTGCCAGCTTTAGTTGAGAGATTTTCTTCCGAGTCTGAGTTCTTAGATCGGCATCTGCCCCACAAAACAGTTGAAGTTGGTGTCTTTAAAATGCCAAGGTTCAAGTATTCTTGTAGATTTGAAGCTTCCAAAGTTTTGAAAACTTTAGGATTGGAGTTGCCTTTTGTTTCTGGAGGTTTGACAGAGATGGTGGACTCGCCTATGAGTGACGGCCTATTTGTTTCCCACATACAACATGGATCATTCATTGATGTTAATGAGGACGGTACAGAAGCTGCTGCTGTTACGGTTGCTCTTCAAACGGGTTCAACTTGGAGTCAGGAGAAGAGGATAGACTTTGTGGCAGATCATCCCTTCCTTTTTCTTATCAGAGAAGAAACCACTGGATCGGTGCTGTTCATTGGGCAAGTCCTCAATCCAGTTGAAGACTGA

Protein Analysis

473

Amino Acids

53.21

Weight (kDa)

6.48

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 89 - 470 7.4e-92 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64020
fragaria_vesca FvH4_1g24600 FvH4_1g24640 FvH4_2g15000 FvH4_3g03700 FvH4_3g03710 FvH4_3g09790 FvH4_3g09790 FvH4_3g09810 FvH4_3g09811 FvH4_3g23720 FvH4_3g32870 FvH4_3g32890 FvH4_3g37550 FvH4_3g37560 FvH4_5g01890 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g21100 FvH4_5g21110 FvH4_5g21330 FvH4_5g21331 FvH4_5g21520 FvH4_5g21540 FvH4_5g21970 FvH4_6g01280
malus_domestica MD00G1084800.v1.1 MD00G1141300.v1.1 MD02G1102800.v1.1 MD02G1282200.v1.1 MD02G1282300.v1.1 MD03G1207900.v1.1 MD03G1230000.v1.1 MD04G1136400.v1.1 MD04G1136500.v1.1 MD04G1189000.v1.1 MD05G1248700.v1.1 MD05G1248800.v1.1 MD06G1030400.v1.1 MD10G1229200.v1.1 MD10G1229300.v1.1 MD15G1291400.v1.1 MD15G1431300.v1.1
prunus_persica Prupe.5G041800_v2.0.a1 Prupe.5G041900_v2.0.a1 Prupe.5G042000_v2.0.a1 Prupe.5G042100_v2.0.a1 Prupe.5G042200_v2.0.a1
pyrus_communis pycom02g14630 pycom02g24090 pycom04g12410 pycom04g16760 pycom05g22550 pycom10g19280 pycom12g05670 pycom15g25490 pycom15g38110
rosa_chinensis RchiOBHm_Chr2g0122001 RchiOBHm_Chr2g0163171 RchiOBHm_Chr2g0163181 RchiOBHm_Chr3g0455201 RchiOBHm_Chr5g0005751 RchiOBHm_Chr5g0015501 RchiOBHm_Chr5g0015511 RchiOBHm_Chr5g0015521 RchiOBHm_Chr5g0015531 RchiOBHm_Chr5g0060021 RchiOBHm_Chr7g0187971 RchiOBHm_Chr7g0207951 RchiOBHm_Chr7g0208671 RchiOBHm_Chr7g0208691 RchiOBHm_Chr7g0208941 RchiOBHm_Chr7g0208951 RchiOBHm_Chr7g0208961
rosa_laevigata RLG00000003132 RLG00000003177 RLG00000003178 RLG00000003179 RLG00000003180 RLG00000003201 RLG00000003202 RLG00000003270 RLG00000004739 RLG00000004741 RLG00000004744 RLG00000021379 RLG00000025378 RLG00000031323 RLG00000031324 RLG00000032205 RLG00000032206 RLG00000035339
rosa_multiflora Rmu_co8189380.1_g000001 Rmu_co8284591.1_g000001 Rmu_co8360283.1_g000002 Rmu_co8379053.1_g000001 Rmu_sc0000493.1_g000007 Rmu_sc0000532.1_g000030 Rmu_sc0000532.1_g000031 Rmu_sc0000570.1_g000033 Rmu_sc0000905.1_g000012 Rmu_sc0001730.1_g000002 Rmu_sc0002045.1_g000039 Rmu_sc0003064.1_g000001 Rmu_sc0003064.1_g000002 Rmu_sc0003064.1_g000006 Rmu_sc0003880.1_g000012 Rmu_sc0004103.1_g000003 Rmu_sc0005082.1_g000007 Rmu_sc0011272.1_g000002 Rmu_sc0015523.1_g000021 Rmu_sc0015523.1_g000022 Rmu_sc0019960.1_g000007 Rmu_sc0029514.1_g000001 Rmu_ssc0000422.1_g000010
rosa_roxburghii Rroxscaffold_1G00013660 Rroxscaffold_1G00060980 Rroxscaffold_1G00060990 Rroxscaffold_1G00061000 Rroxscaffold_1G00061010 Rroxscaffold_2G00087490 Rroxscaffold_2G00122080 Rroxscaffold_2G00123490 Rroxscaffold_3G00249930 Rroxscaffold_3G00249940 Rroxscaffold_3G00249950 Rroxscaffold_3G00249980 Rroxscaffold_3G00250310 Rroxscaffold_3G00250320 Rroxscaffold_3G00266850 Rroxscaffold_3G00266870 Rroxscaffold_3G00266910 Rroxscaffold_6G00424480
rosa_rugosa Rorug02G0228900 Rorug02G0236000 Rorug02G0501100 Rorug03G0004700 Rorug03G0004700 Rorug03G0004800 Rorug04G0417800 Rorug05G0027800 Rorug05G0027900 Rorug05G0028000 Rorug05G0327700 Rorug05G0384800 Rorug06G0486000 Rorug06G0486100 Rorug06G0486200 Rorug06G0486200 Rorug06G0486300 Rorug06G0486400 Rorug06G0486500 Rorug07G0100400 Rorug07G0105400 Rorug07G0105400 Rorug07G0107300 Rorug07G0107400 Rorug07G0107500 Rorug07G0113300
rosa_samantha Rh7AG091300 Rh7BG092800 Rh7CG091600 Rh7CG257500 Rh7DG093500
rosa_wichuraiana Rw0G001260 Rw0G017680 Rw2G023470 Rw3G005130 Rw5G004560 Rw5G010480 Rw7G020400 Rw7G020410 Rw7G020540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 540
AccII CGCG 1 cut(s) 485
AciI CCGC 4 cut(s) 344, 377, 504, 880
AclWI GGATC 4 cut(s) 161, 199, 1237, 1384
AcoI YGGCCR 3 cut(s) 25, 58, 378
AcsI RAATTY 2 cut(s) 49, 655
AcuI CTGAAG 2 cut(s) 333, 965
AfaI GTAC 2 cut(s) 338, 1258
AfiI CCNNNNNNNGG 5 cut(s) 34, 271, 376, 400, 503
AflII CTTAAG 1 cut(s) 422
AflIII ACRYGT 1 cut(s) 543
AhdI GACNNNNNGTC 1 cut(s) 681
AjnI CCWGG 1 cut(s) 706
AleI CACNNNNGTG 1 cut(s) 272
AluBI AGCT 7 cut(s) 298, 445, 513, 870, 998, 1115, 1265
AluI AGCT 7 cut(s) 298, 445, 513, 870, 998, 1115, 1265
Alw21I GWGCWC 2 cut(s) 311, 447
Alw26I GTCTC 2 cut(s) 552, 668
AlwI GGATC 4 cut(s) 161, 199, 1237, 1384
AlwNI CAGNNNCTG 1 cut(s) 1265
AoxI GGCC 8 cut(s) 25, 36, 58, 378, 506, 663, 934, 1201
ApeKI GCWGC 5 cut(s) 5, 295, 599, 1265, 1268
ApoI RAATTY 2 cut(s) 49, 655
ArsI GACNNNNNNTTYG 2 cut(s) 1190, 1222
Asp700I GAANNNNTTC 1 cut(s) 950
AspLEI GCGC 1 cut(s) 533
AspS9I GGNCC 4 cut(s) 37, 403, 506, 663
AsuC2I CCSGG 1 cut(s) 382
AsuHPI GGTGA 4 cut(s) 406, 486, 514, 806
AvaII GGWCC 1 cut(s) 403
BanII GRGCYC 3 cut(s) 158, 447, 833
BbsI GAAGAC 1 cut(s) 780
Bbv12I GWGCWC 2 cut(s) 311, 447
BbvI GCAGC 5 cut(s) 17, 307, 586, 1252, 1255
BccI CCATC 4 cut(s) 69, 359, 980, 1171
BceAI ACGGC 2 cut(s) 12, 1216
BciT130I CCWGG 1 cut(s) 708
BcnI CCSGG 1 cut(s) 382
BcoDI GTCTC 2 cut(s) 552, 668
BfaI CTAG 3 cut(s) 455, 510, 729
BfoI RGCGCY 1 cut(s) 534
BfrI CTTAAG 1 cut(s) 422
BglII AGATCT 1 cut(s) 940
BisI GCNGC 7 cut(s) 6, 296, 344, 378, 600, 1266, 1269
BlsI GCNGC 7 cut(s) 7, 297, 345, 379, 601, 1267, 1270
Bme1390I CCNGG 2 cut(s) 382, 708
Bme18I GGWCC 1 cut(s) 403
BmeRI GACNNNNNGTC 1 cut(s) 681
BmgT120I GGNCC 4 cut(s) 37, 403, 506, 663
BmrFI CCNGG 2 cut(s) 382, 708
BmsI GCATC 4 cut(s) 696, 775, 797, 1051
BpiI GAAGAC 1 cut(s) 780
BpmI CTGGAG 1 cut(s) 1182
BpuMI CCSGG 1 cut(s) 382
BsaBI GATNNNNATC 1 cut(s) 1041
BsaJI CCNNGG 4 cut(s) 22, 209, 270, 1085
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 271, 376, 400, 503
Bse118I RCCGGY 1 cut(s) 70
Bse1I ACTGG 3 cut(s) 406, 1378, 1409
Bse8I GATNNNNATC 1 cut(s) 1041
BseBI CCWGG 1 cut(s) 708
BseDI CCNNGG 4 cut(s) 22, 209, 270, 1085
BseGI GGATG 3 cut(s) 351, 640, 1339
BseJI GATNNNNATC 1 cut(s) 1041
BseLI CCNNNNNNNGG 5 cut(s) 34, 271, 376, 400, 503
BseMII CTCAG 3 cut(s) 379, 846, 1017
BseNI ACTGG 3 cut(s) 406, 1378, 1409
BseRI GAGGAG 1 cut(s) 11
BseX3I CGGCCG 2 cut(s) 25, 378
BseXI GCAGC 5 cut(s) 17, 307, 586, 1252, 1255
BseYI CCCAGC 1 cut(s) 870
Bsh1236I CGCG 1 cut(s) 485
Bsh1285I CGRYCG 2 cut(s) 28, 381
BshFI GGCC 8 cut(s) 27, 38, 60, 380, 508, 665, 936, 1203
BsiEI CGRYCG 2 cut(s) 28, 381
BsiHKAI GWGCWC 2 cut(s) 311, 447
BsiSI CCGG 3 cut(s) 57, 71, 381
BslI CCNNNNNNNGG 5 cut(s) 34, 271, 376, 400, 503
BsmAI GTCTC 2 cut(s) 552, 668
BsnI GGCC 8 cut(s) 27, 38, 60, 380, 508, 665, 936, 1203
Bsp1286I GDGCHC 4 cut(s) 158, 311, 447, 833
Bsp143I GATC 8 cut(s) 139, 166, 204, 940, 1036, 1229, 1336, 1376
Bsp68I TCGCGA 1 cut(s) 485
BspACI CCGC 4 cut(s) 344, 377, 504, 880
BspANI GGCC 8 cut(s) 27, 38, 60, 380, 508, 665, 936, 1203
BspCNI CTCAG 3 cut(s) 378, 845, 1018
BspFNI CGCG 1 cut(s) 485
BspHI TCATGA 2 cut(s) 288, 849
BspPI GGATC 4 cut(s) 161, 199, 1237, 1384
BspQI GCTCTTC 1 cut(s) 1290
BspTI CTTAAG 1 cut(s) 422
BsrFI RCCGGY 1 cut(s) 70
BsrI ACTGG 3 cut(s) 406, 1378, 1409
BssAI RCCGGY 1 cut(s) 70
BssECI CCNNGG 4 cut(s) 22, 209, 270, 1085
BssMI GATC 8 cut(s) 139, 166, 204, 940, 1036, 1229, 1336, 1376
BssT1I CCWWGG 1 cut(s) 1085
Bst2UI CCWGG 1 cut(s) 708
Bst4CI ACNGT 3 cut(s) 1060, 1256, 1279
Bst6I CTCTTC 4 cut(s) 555, 941, 1290, 1310
BstAFI CTTAAG 1 cut(s) 422
BstC8I GCNNGC 2 cut(s) 506, 996
BstDEI CTNAG 4 cut(s) 365, 832, 1026, 1033
BstDSI CCRYGG 1 cut(s) 209
BstF5I GGATG 3 cut(s) 351, 640, 1339
BstFNI CGCG 1 cut(s) 485
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 1 cut(s) 533
BstKTI GATC 8 cut(s) 142, 169, 207, 943, 1039, 1232, 1339, 1379
BstMAI GTCTC 2 cut(s) 552, 668
BstMBI GATC 8 cut(s) 139, 166, 204, 940, 1036, 1229, 1336, 1376
BstMCI CGRYCG 2 cut(s) 28, 381
BstMWI GCNNNNNNNGC 2 cut(s) 510, 596
BstNI CCWGG 1 cut(s) 708
BstNSI RCATGY 1 cut(s) 547
BstSCI CCNGG 2 cut(s) 380, 706
BstUI CGCG 1 cut(s) 485
BstV1I GCAGC 5 cut(s) 17, 307, 586, 1252, 1255
BstV2I GAAGAC 1 cut(s) 780
BstX2I RGATCY 1 cut(s) 940
BstXI CCANNNNNNTGG 1 cut(s) 358
BstYI RGATCY 1 cut(s) 940
BstZI CGGCCG 2 cut(s) 25, 378
BsuRI GGCC 8 cut(s) 27, 38, 60, 380, 508, 665, 936, 1203
BtgI CCRYGG 1 cut(s) 209
BtsCI GGATG 3 cut(s) 351, 640, 1339
BtsI GCAGTG 1 cut(s) 585
BtsIMutI CAGTG 3 cut(s) 309, 585, 1371
BtuMI TCGCGA 1 cut(s) 485
Cac8I GCNNGC 2 cut(s) 506, 996
CaiI CAGNNNCTG 1 cut(s) 1265
CciI TCATGA 2 cut(s) 288, 849
CfoI GCGC 1 cut(s) 533
Cfr10I RCCGGY 1 cut(s) 70
Cfr13I GGNCC 4 cut(s) 37, 403, 506, 663
Csp6I GTAC 2 cut(s) 337, 1257
CviAII CATG 8 cut(s) 289, 358, 469, 544, 660, 850, 958, 1226
CviQI GTAC 2 cut(s) 337, 1257
DdeI CTNAG 4 cut(s) 365, 832, 1026, 1033
DpnI GATC 8 cut(s) 141, 168, 206, 942, 1038, 1231, 1338, 1378
DpnII GATC 8 cut(s) 139, 166, 204, 940, 1036, 1229, 1336, 1376
DraI TTTAAA 1 cut(s) 1078
DriI GACNNNNNGTC 1 cut(s) 681
EaeI YGGCCR 3 cut(s) 25, 58, 378
EagI CGGCCG 2 cut(s) 25, 378
Eam1104I CTCTTC 4 cut(s) 555, 941, 1290, 1310
Eam1105I GACNNNNNGTC 1 cut(s) 681
EarI CTCTTC 4 cut(s) 555, 941, 1290, 1310
Ecl136II GAGCTC 1 cut(s) 445
EclXI CGGCCG 2 cut(s) 25, 378
Eco130I CCWWGG 1 cut(s) 1085
Eco24I GRGCYC 3 cut(s) 158, 447, 833
Eco47I GGWCC 1 cut(s) 403
Eco52I CGGCCG 2 cut(s) 25, 378
Eco53kI GAGCTC 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 333, 965
EcoICRI GAGCTC 1 cut(s) 445
EcoO109I RGGNCCY 1 cut(s) 37
EcoRI GAATTC 1 cut(s) 655
EcoRII CCWGG 1 cut(s) 706
EcoT14I CCWWGG 1 cut(s) 1085
EcoT22I ATGCAT 2 cut(s) 751, 790
EcoT38I GRGCYC 3 cut(s) 158, 447, 833
ErhI CCWWGG 1 cut(s) 1085
FaeI CATG 8 cut(s) 292, 361, 472, 547, 663, 853, 961, 1229
FalI AAGNNNNNCTT 2 cut(s) 684, 716
FatI CATG 8 cut(s) 288, 357, 468, 543, 659, 849, 957, 1225
FauI CCCGC 1 cut(s) 497
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 7 cut(s) 6, 296, 344, 378, 600, 1266, 1269
FokI GGATG 3 cut(s) 338, 647, 1326
FriOI GRGCYC 3 cut(s) 158, 447, 833
Fsp4HI GCNGC 7 cut(s) 6, 296, 344, 378, 600, 1266, 1269
FspBI CTAG 3 cut(s) 455, 510, 729
GlaI GCGC 1 cut(s) 532
GluI GCNGC 7 cut(s) 6, 296, 344, 378, 600, 1266, 1269
GsaI CCCAGC 1 cut(s) 874
GsuI CTGGAG 1 cut(s) 1182
HaeII RGCGCY 1 cut(s) 534
HaeIII GGCC 8 cut(s) 27, 38, 60, 380, 508, 665, 936, 1203
HapII CCGG 3 cut(s) 57, 71, 381
HhaI GCGC 1 cut(s) 533
Hin1II CATG 8 cut(s) 292, 361, 472, 547, 663, 853, 961, 1229
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HindIII AAGCTT 1 cut(s) 1113
HinfI GANTC 4 cut(s) 491, 1022, 1184, 1306
HpaII CCGG 3 cut(s) 57, 71, 381
HphI GGTGA 4 cut(s) 406, 486, 514, 806
Hpy166II GTNNAC 7 cut(s) 32, 337, 435, 541, 583, 718, 1183
Hpy188I TCNGA 9 cut(s) 139, 166, 204, 634, 945, 975, 1021, 1027, 1361
Hpy188III TCNNGA 7 cut(s) 289, 484, 792, 838, 850, 1161, 1310
Hpy8I GTNNAC 7 cut(s) 32, 337, 435, 541, 583, 718, 1183
HpyAV CCTTC 5 cut(s) 427, 573, 664, 827, 1354
HpyCH4III ACNGT 3 cut(s) 1060, 1256, 1279
HpyCH4V TGCA 2 cut(s) 749, 788
HpyF10VI GCNNNNNNNGC 2 cut(s) 510, 596
HpyF3I CTNAG 4 cut(s) 365, 832, 1026, 1033
Hsp92II CATG 8 cut(s) 292, 361, 472, 547, 663, 853, 961, 1229
HspAI GCGC 1 cut(s) 531
Kzo9I GATC 8 cut(s) 139, 166, 204, 940, 1036, 1229, 1336, 1376
LguI GCTCTTC 1 cut(s) 1290
LmnI GCTCC 2 cut(s) 709, 867
Lsp1109I GCAGC 5 cut(s) 17, 307, 586, 1252, 1255
LweI GCATC 4 cut(s) 696, 775, 797, 1051
MaeI CTAG 3 cut(s) 455, 510, 729
MaeIII GTNAC 2 cut(s) 1196, 1273
MalI GATC 8 cut(s) 141, 168, 206, 942, 1038, 1231, 1338, 1378
MboI GATC 8 cut(s) 139, 166, 204, 940, 1036, 1229, 1336, 1376
MflI RGATCY 1 cut(s) 940
MhlI GDGCHC 4 cut(s) 158, 311, 447, 833
MluCI AATT 2 cut(s) 49, 655
MlyI GAGTC 3 cut(s) 1031, 1178, 1315
MmeI TCCRAC 1 cut(s) 612
Mph1103I ATGCAT 2 cut(s) 751, 790
MroXI GAANNNNTTC 1 cut(s) 950
MseI TTAA 4 cut(s) 423, 605, 1077, 1245
MslI CAYNNNNRTG 4 cut(s) 272, 356, 467, 962
MspA1I CMGCKG 1 cut(s) 298
MspCI CTTAAG 1 cut(s) 422
MspI CCGG 3 cut(s) 57, 71, 381
MspR9I CCNGG 2 cut(s) 382, 708
MvaI CCWGG 1 cut(s) 708
MvnI CGCG 1 cut(s) 485
MwoI GCNNNNNNNGC 2 cut(s) 510, 596
NciI CCSGG 1 cut(s) 382
NdeII GATC 8 cut(s) 139, 166, 204, 940, 1036, 1229, 1336, 1376
NlaIII CATG 8 cut(s) 292, 361, 472, 547, 663, 853, 961, 1229
NmeAIII GCCGAG 1 cut(s) 691
NmuCI GTSAC 1 cut(s) 1196
NruI TCGCGA 1 cut(s) 485
NsiI ATGCAT 2 cut(s) 751, 790
NspI RCATGY 1 cut(s) 547
OliI CACNNNNGTG 1 cut(s) 272
PagI TCATGA 2 cut(s) 288, 849
PciI ACATGT 1 cut(s) 543
PciSI GCTCTTC 1 cut(s) 1290
PdmI GAANNNNTTC 1 cut(s) 950
PfeI GAWTC 1 cut(s) 491
PkrI GCNGC 7 cut(s) 7, 297, 345, 379, 601, 1267, 1270
PleI GAGTC 3 cut(s) 1030, 1178, 1314
PpsI GAGTC 3 cut(s) 1030, 1178, 1314
PscI ACATGT 1 cut(s) 543
Psp124BI GAGCTC 1 cut(s) 447
Psp6I CCWGG 1 cut(s) 706
PspFI CCCAGC 1 cut(s) 870
PspGI CCWGG 1 cut(s) 706
PspPI GGNCC 4 cut(s) 37, 403, 506, 663
PstNI CAGNNNCTG 1 cut(s) 1265
PsuI RGATCY 1 cut(s) 940
PvuII CAGCTG 1 cut(s) 298
RruI TCGCGA 1 cut(s) 485
RsaI GTAC 2 cut(s) 338, 1258
RsaNI GTAC 2 cut(s) 337, 1257
RseI CAYNNNNRTG 4 cut(s) 272, 356, 467, 962
SacI GAGCTC 1 cut(s) 447
SapI GCTCTTC 1 cut(s) 1290
SaqAI TTAA 4 cut(s) 423, 605, 1077, 1245
SatI GCNGC 7 cut(s) 6, 296, 344, 378, 600, 1266, 1269
Sau3AI GATC 8 cut(s) 139, 166, 204, 940, 1036, 1229, 1336, 1376
Sau96I GGNCC 4 cut(s) 37, 403, 506, 663
SchI GAGTC 3 cut(s) 1031, 1178, 1315
ScrFI CCNGG 2 cut(s) 382, 708
SduI GDGCHC 4 cut(s) 158, 311, 447, 833
SfaNI GCATC 4 cut(s) 696, 775, 797, 1051
SinI GGWCC 1 cut(s) 403
SmiMI CAYNNNNRTG 4 cut(s) 272, 356, 467, 962
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 2 cut(s) 49, 655
SsiI CCGC 4 cut(s) 344, 377, 504, 880
SspMI CTAG 3 cut(s) 455, 510, 729
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 2 cut(s) 380, 706
StyI CCWWGG 1 cut(s) 1085
TaaI ACNGT 3 cut(s) 1060, 1256, 1279
TaqI TCGA 1 cut(s) 615
TasI AATT 2 cut(s) 49, 655
TatI WGTACW 1 cut(s) 336
TauI GCSGC 2 cut(s) 346, 380
TfiI GAWTC 1 cut(s) 491
Tru1I TTAA 4 cut(s) 423, 605, 1077, 1245
Tru9I TTAA 4 cut(s) 423, 605, 1077, 1245
TscAI CASTG 3 cut(s) 316, 592, 1378
TseFI GTSAC 1 cut(s) 1196
TseI GCWGC 5 cut(s) 5, 295, 599, 1265, 1268
Tsp45I GTSAC 1 cut(s) 1196
TspDTI ATGAA 9 cut(s) 236, 305, 648, 651, 724, 838, 993, 1225, 1378
TspGWI ACGGA 2 cut(s) 32, 589
TspRI CASTG 3 cut(s) 316, 592, 1378
Vha464I CTTAAG 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 403
XapI RAATTY 2 cut(s) 49, 655
XceI RCATGY 1 cut(s) 547
XcmI CCANNNNNNNNNTGG 1 cut(s) 1223
XmiI GTMKAC 1 cut(s) 540
XmnI GAANNNNTTC 1 cut(s) 950
XspI CTAG 3 cut(s) 455, 510, 729
Zsp2I ATGCAT 2 cut(s) 751, 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.