Rw5G010480

Belongs to the serpin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000163
Physical Location & Seq
Reverse (-)
11507047 .. 11508249
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G010480.1

Sequence Viewer

Length: 1203 bp
ATGGATCTCCAAAAGCCAATGGTCCCGGACGCAAACAAACGTAAACGAAAAACGAAAACAAAAACAACACCAATGGATCTCGAAGAATCCATCAGCAACCAAACTGACGTCGGACTGGAGATAACGAAGCAACTGCTCCAAACTGAGTTCAAAGACAAGAACATGGTGTACTCCCCAGTATCTATCCACATCGTGCTGAGTCTAATAGCAGCTGGGAAAACTAGTTCCGATCTGGACCAGTTTCTGTCTTTCCTCAAGTCCAAGTCCGTCGACGACCTCAACTCCCTCGCCTACAATCTCGTAACTTCCATTTTGGCGGATGCATCCCGCCGTGGCGGGCCATGTTTGAACTTCGCCAATGGTCTCTGGGTCGACGAGTCTCTCCCACTGGATCATTCTTACGAGAAGGTTCTGTCCACTTCCTACAAGGCGGCTCTAAACCACGTCGATTTCAAATCCAACCCCGAGGGAGTGAGAACCCAAGTGAATTCCTGGATCAACAAGGAGACCAATGGCCTTATCCCTGAGATTCTTTCTCCGCACTCAGTCACCAGCCAAACGAGTCGCATCTTCGCAAACGCCTTATACTTCAAAGCAACTTGGGACGATGAGTACTTTGACGCATCAAGAACCAGAAAAGGTGAGTTTCACCTTCTGAACGGGGATTTAGTTAAGGGAGTGCGTTTCATGACCAGCGGTCATCGCCACTCCATAAGTGCCTATGATGGTTTTAAAGTCTTGAGTCTTCCTTACAAACCAAGTCTAGATTACAGGGACATGCGAGGTTTTTATATGCACTTGCTTCTTCCAGATGCAAGAGATGGGCTGCCAGCTCTGGCCGAGAGGGTTTGTTCAGAGTCCGGGTTTTTAGATCGTCATCTTCAATGGAATGAGGTTAAAGTGCGGAAATTGTTGATCCCAAAGTTTAAGATCTCATCTAGGTTTGAGGCCTCCGGTGTTATGGAGAAACTAGGGCTGCCGGCTGATGCCATGGTGATGATGCATGAAGCCTTGATTGAAGTTGATGAAAATGGCACAACAGCTACAGCTGCTACTGTTGCCCAGTCATACCGGGCTCTTGCTCCGCCGCCTGAAGTGAAAGAAGAAAACTTTGTGGCTGACCACCCATTCATGTTTCTCATTGAAGAAGGTCGTGGAACGATGCTGTTCATGGGGCATGTGCTCAATCCAGTTGCTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05146 ko05164 map03013 map05146 map05164
Pfam Domains
Protein Families
Serpin

Protein Analysis

400

Amino Acids

44.77

Weight (kDa)

6.33

Isoelectric Point (pI)

34.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 33 - 397 2.9e-70 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64020
fragaria_vesca FvH4_1g24600 FvH4_1g24640 FvH4_2g15000 FvH4_3g03700 FvH4_3g03710 FvH4_3g09790 FvH4_3g09790 FvH4_3g09810 FvH4_3g09811 FvH4_3g23720 FvH4_3g32870 FvH4_3g32890 FvH4_3g37550 FvH4_3g37560 FvH4_5g01890 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g21100 FvH4_5g21110 FvH4_5g21330 FvH4_5g21331 FvH4_5g21520 FvH4_5g21540 FvH4_5g21970 FvH4_6g01280
malus_domestica MD00G1084800.v1.1 MD00G1141300.v1.1 MD02G1102800.v1.1 MD02G1282200.v1.1 MD02G1282300.v1.1 MD03G1207900.v1.1 MD03G1230000.v1.1 MD04G1136400.v1.1 MD04G1136500.v1.1 MD04G1189000.v1.1 MD05G1248700.v1.1 MD05G1248800.v1.1 MD06G1030400.v1.1 MD10G1229200.v1.1 MD10G1229300.v1.1 MD15G1291400.v1.1 MD15G1431300.v1.1
prunus_persica Prupe.5G041800_v2.0.a1 Prupe.5G041900_v2.0.a1 Prupe.5G042000_v2.0.a1 Prupe.5G042100_v2.0.a1 Prupe.5G042200_v2.0.a1
pyrus_communis pycom02g14630 pycom02g24090 pycom04g12410 pycom04g16760 pycom05g22550 pycom10g19280 pycom12g05670 pycom15g25490 pycom15g38110
rosa_chinensis RchiOBHm_Chr2g0122001 RchiOBHm_Chr2g0163171 RchiOBHm_Chr2g0163181 RchiOBHm_Chr3g0455201 RchiOBHm_Chr5g0005751 RchiOBHm_Chr5g0015501 RchiOBHm_Chr5g0015511 RchiOBHm_Chr5g0015521 RchiOBHm_Chr5g0015531 RchiOBHm_Chr5g0060021 RchiOBHm_Chr7g0187971 RchiOBHm_Chr7g0207951 RchiOBHm_Chr7g0208671 RchiOBHm_Chr7g0208691 RchiOBHm_Chr7g0208941 RchiOBHm_Chr7g0208951 RchiOBHm_Chr7g0208961
rosa_laevigata RLG00000003132 RLG00000003177 RLG00000003178 RLG00000003179 RLG00000003180 RLG00000003201 RLG00000003202 RLG00000003270 RLG00000004739 RLG00000004741 RLG00000004744 RLG00000021379 RLG00000025378 RLG00000031323 RLG00000031324 RLG00000032205 RLG00000032206 RLG00000035339
rosa_multiflora Rmu_co8189380.1_g000001 Rmu_co8284591.1_g000001 Rmu_co8360283.1_g000002 Rmu_co8379053.1_g000001 Rmu_sc0000493.1_g000007 Rmu_sc0000532.1_g000030 Rmu_sc0000532.1_g000031 Rmu_sc0000570.1_g000033 Rmu_sc0000905.1_g000012 Rmu_sc0001730.1_g000002 Rmu_sc0002045.1_g000039 Rmu_sc0003064.1_g000001 Rmu_sc0003064.1_g000002 Rmu_sc0003064.1_g000006 Rmu_sc0003880.1_g000012 Rmu_sc0004103.1_g000003 Rmu_sc0005082.1_g000007 Rmu_sc0011272.1_g000002 Rmu_sc0015523.1_g000021 Rmu_sc0015523.1_g000022 Rmu_sc0019960.1_g000007 Rmu_sc0029514.1_g000001 Rmu_ssc0000422.1_g000010
rosa_roxburghii Rroxscaffold_1G00013660 Rroxscaffold_1G00060980 Rroxscaffold_1G00060990 Rroxscaffold_1G00061000 Rroxscaffold_1G00061010 Rroxscaffold_2G00087490 Rroxscaffold_2G00122080 Rroxscaffold_2G00123490 Rroxscaffold_3G00249930 Rroxscaffold_3G00249940 Rroxscaffold_3G00249950 Rroxscaffold_3G00249980 Rroxscaffold_3G00250310 Rroxscaffold_3G00250320 Rroxscaffold_3G00266850 Rroxscaffold_3G00266870 Rroxscaffold_3G00266910 Rroxscaffold_6G00424480
rosa_rugosa Rorug02G0228900 Rorug02G0236000 Rorug02G0501100 Rorug03G0004700 Rorug03G0004700 Rorug03G0004800 Rorug04G0417800 Rorug05G0027800 Rorug05G0027900 Rorug05G0028000 Rorug05G0327700 Rorug05G0384800 Rorug06G0486000 Rorug06G0486100 Rorug06G0486200 Rorug06G0486200 Rorug06G0486300 Rorug06G0486400 Rorug06G0486500 Rorug07G0100400 Rorug07G0105400 Rorug07G0105400 Rorug07G0107300 Rorug07G0107400 Rorug07G0107500 Rorug07G0113300
rosa_samantha Rh7AG091300 Rh7BG092800 Rh7CG091600 Rh7CG257500 Rh7DG093500
rosa_wichuraiana Rw0G001260 Rw0G017680 Rw2G023470 Rw3G005130 Rw5G004560 Rw5G010480 Rw7G020400 Rw7G020410 Rw7G020540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 111
AccB7I CCANNNNNTGG 1 cut(s) 1196
AccI GTMKAC 2 cut(s) 270, 372
AciI CCGC 9 cut(s) 317, 328, 336, 431, 539, 696, 904, 1085, 1088
AclWI GGATC 5 cut(s) 12, 84, 399, 503, 910
AcoI YGGCCR 1 cut(s) 837
AcsI RAATTY 1 cut(s) 487
AcuI CTGAAG 1 cut(s) 1113
AcyI GRCGYC 1 cut(s) 108
AdeI CACNNNGTG 1 cut(s) 193
AfaI GTAC 2 cut(s) 170, 614
AfiI CCNNNNNNNGG 1 cut(s) 1196
AgsI TTSAA 7 cut(s) 151, 349, 454, 592, 884, 1019, 1145
AhdI GACNNNNNGTC 1 cut(s) 696
AhlI ACTAGT 1 cut(s) 221
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 1 cut(s) 491
AloI GAACNNNNNNTCC 2 cut(s) 208, 240
AluBI AGCT 4 cut(s) 212, 833, 1043, 1049
AluI AGCT 4 cut(s) 212, 833, 1043, 1049
Alw21I GWGCWC 1 cut(s) 1185
Alw26I GTCTC 3 cut(s) 368, 384, 500
AlwI GGATC 5 cut(s) 12, 84, 399, 503, 910
AlwNI CAGNNNCTG 2 cut(s) 244, 1196
Ama87I CYCGRG 1 cut(s) 464
AoxI GGCC 4 cut(s) 338, 514, 837, 948
ApeKI GCWGC 4 cut(s) 209, 826, 976, 1049
ApoI RAATTY 1 cut(s) 487
AspS9I GGNCC 3 cut(s) 22, 235, 338
AsuC2I CCSGG 3 cut(s) 26, 862, 1073
AsuHPI GGTGA 4 cut(s) 541, 641, 653, 1006
AvaI CYCGRG 1 cut(s) 464
AvaII GGWCC 2 cut(s) 22, 235
BanII GRGCYC 1 cut(s) 1078
BbsI GAAGAC 1 cut(s) 737
Bbv12I GWGCWC 1 cut(s) 1185
BbvI GCAGC 4 cut(s) 221, 813, 963, 1036
BccI CCATC 3 cut(s) 98, 719, 815
BceAI ACGGC 1 cut(s) 315
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 1 cut(s) 493
BcnI CCSGG 3 cut(s) 26, 862, 1073
BcoDI GTCTC 3 cut(s) 368, 384, 500
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 4 cut(s) 222, 764, 939, 971
BfmI CTRYAG 1 cut(s) 1044
BglII AGATCT 1 cut(s) 930
BisI GCNGC 6 cut(s) 210, 432, 827, 977, 1050, 1088
BlsI GCNGC 6 cut(s) 211, 433, 828, 978, 1051, 1089
BmcAI AGTACT 1 cut(s) 614
Bme1390I CCNGG 4 cut(s) 26, 493, 862, 1073
Bme18I GGWCC 2 cut(s) 22, 235
BmeRI GACNNNNNGTC 1 cut(s) 696
BmeT110I CYCGRG 1 cut(s) 464
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 3 cut(s) 22, 235, 338
BmiI GGNNCC 1 cut(s) 24
BmrFI CCNGG 4 cut(s) 26, 493, 862, 1073
BmrI ACTGGG 2 cut(s) 170, 1057
BmsI GCATC 8 cut(s) 310, 332, 576, 632, 802, 976, 990, 1152
BmuI ACTGGG 2 cut(s) 170, 1057
BpiI GAAGAC 1 cut(s) 737
BpmI CTGGAG 1 cut(s) 137
BpuEI CTTGAG 2 cut(s) 239, 760
BpuMI CCSGG 3 cut(s) 26, 862, 1073
BsaBI GATNNNNATC 1 cut(s) 876
BsaHI GRCGYC 1 cut(s) 108
BsaI GGTCTC 2 cut(s) 368, 500
BsaJI CCNNGG 3 cut(s) 331, 465, 990
BsaWI WCCGGW 1 cut(s) 953
BsaXI ACNNNNNCTCC 2 cut(s) 266, 296
Bsc4I CCNNNNNNNGG 1 cut(s) 1196
Bse118I RCCGGY 1 cut(s) 979
Bse1I ACTGG 6 cut(s) 120, 176, 238, 393, 1063, 1190
Bse8I GATNNNNATC 1 cut(s) 876
BseBI CCWGG 1 cut(s) 493
BseDI CCNNGG 3 cut(s) 331, 465, 990
BseGI GGATG 2 cut(s) 323, 325
BseJI GATNNNNATC 1 cut(s) 876
BseLI CCNNNNNNNGG 1 cut(s) 1196
BseMII CTCAG 4 cut(s) 135, 188, 516, 558
BseNI ACTGG 6 cut(s) 120, 176, 238, 393, 1063, 1190
BseXI GCAGC 4 cut(s) 221, 813, 963, 1036
BseYI CCCAGC 1 cut(s) 212
BshFI GGCC 4 cut(s) 340, 516, 839, 950
BsiHKAI GWGCWC 1 cut(s) 1185
BsiHKCI CYCGRG 1 cut(s) 464
BsiSI CCGG 5 cut(s) 26, 861, 954, 980, 1072
BslFI GGGAC 3 cut(s) 8, 617, 788
BslI CCNNNNNNNGG 1 cut(s) 1196
BsmAI GTCTC 3 cut(s) 368, 384, 500
BsmFI GGGAC 3 cut(s) 8, 617, 788
BsnI GGCC 4 cut(s) 340, 516, 839, 950
Bso31I GGTCTC 2 cut(s) 368, 500
BsoBI CYCGRG 1 cut(s) 464
Bsp1286I GDGCHC 2 cut(s) 1078, 1185
Bsp143I GATC 8 cut(s) 4, 76, 229, 391, 495, 871, 915, 930
Bsp19I CCATGG 1 cut(s) 990
BspACI CCGC 9 cut(s) 317, 328, 336, 431, 539, 696, 904, 1085, 1088
BspANI GGCC 4 cut(s) 340, 516, 839, 950
BspCNI CTCAG 4 cut(s) 136, 189, 517, 557
BspHI TCATGA 1 cut(s) 687
BspLI GGNNCC 1 cut(s) 24
BspPI GGATC 5 cut(s) 12, 84, 399, 503, 910
BspTNI GGTCTC 2 cut(s) 368, 500
BsrFI RCCGGY 1 cut(s) 979
BsrI ACTGG 6 cut(s) 120, 176, 238, 393, 1063, 1190
BssAI RCCGGY 1 cut(s) 979
BssECI CCNNGG 3 cut(s) 331, 465, 990
BssMI GATC 8 cut(s) 4, 76, 229, 391, 495, 871, 915, 930
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 1 cut(s) 990
Bst2UI CCWGG 1 cut(s) 493
Bst4CI ACNGT 1 cut(s) 1057
BstACI GRCGYC 1 cut(s) 108
BstC8I GCNNGC 4 cut(s) 338, 831, 981, 1198
BstDEI CTNAG 4 cut(s) 144, 197, 525, 544
BstDSI CCRYGG 2 cut(s) 331, 990
BstF5I GGATG 2 cut(s) 323, 325
BstKTI GATC 8 cut(s) 7, 79, 232, 394, 498, 874, 918, 933
BstMAI GTCTC 3 cut(s) 368, 384, 500
BstMBI GATC 8 cut(s) 4, 76, 229, 391, 495, 871, 915, 930
BstMWI GCNNNNNNNGC 3 cut(s) 702, 1049, 1058
BstNI CCWGG 1 cut(s) 493
BstNSI RCATGY 2 cut(s) 781, 1181
BstSCI CCNGG 4 cut(s) 24, 491, 860, 1071
BstSFI CTRYAG 1 cut(s) 1044
BstV1I GCAGC 4 cut(s) 221, 813, 963, 1036
BstV2I GAAGAC 1 cut(s) 737
BstX2I RGATCY 3 cut(s) 4, 76, 930
BstYI RGATCY 3 cut(s) 4, 76, 930
BsuRI GGCC 4 cut(s) 340, 516, 839, 950
BtgI CCRYGG 2 cut(s) 331, 990
BtgZI GCGATG 1 cut(s) 686
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 2 cut(s) 323, 325
BtsIMutI CAGTG 1 cut(s) 386
Cac8I GCNNGC 4 cut(s) 338, 831, 981, 1198
CaiI CAGNNNCTG 2 cut(s) 244, 1196
CciI TCATGA 1 cut(s) 687
Cfr10I RCCGGY 1 cut(s) 979
Cfr13I GGNCC 3 cut(s) 22, 235, 338
CseI GACGC 2 cut(s) 38, 629
Csp6I GTAC 2 cut(s) 169, 613
CviAII CATG 9 cut(s) 163, 342, 688, 778, 991, 1004, 1132, 1171, 1178
CviQI GTAC 2 cut(s) 169, 613
DdeI CTNAG 4 cut(s) 144, 197, 525, 544
DpnI GATC 8 cut(s) 6, 78, 231, 393, 497, 873, 917, 932
DpnII GATC 8 cut(s) 4, 76, 229, 391, 495, 871, 915, 930
DraI TTTAAA 1 cut(s) 733
DraIII CACNNNGTG 1 cut(s) 193
DriI GACNNNNNGTC 1 cut(s) 696
EaeI YGGCCR 1 cut(s) 837
Eam1105I GACNNNNNGTC 1 cut(s) 696
EciI GGCGGA 2 cut(s) 332, 1074
Eco130I CCWWGG 1 cut(s) 990
Eco147I AGGCCT 1 cut(s) 950
Eco24I GRGCYC 1 cut(s) 1078
Eco31I GGTCTC 2 cut(s) 368, 500
Eco47I GGWCC 2 cut(s) 22, 235
Eco57I CTGAAG 1 cut(s) 1113
Eco88I CYCGRG 1 cut(s) 464
EcoRI GAATTC 1 cut(s) 487
EcoRII CCWGG 1 cut(s) 491
EcoT14I CCWWGG 1 cut(s) 990
EcoT22I ATGCAT 2 cut(s) 325, 1005
EcoT38I GRGCYC 1 cut(s) 1078
ErhI CCWWGG 1 cut(s) 990
FaeI CATG 9 cut(s) 166, 345, 691, 781, 994, 1007, 1135, 1174, 1181
FaqI GGGAC 3 cut(s) 8, 617, 788
FatI CATG 9 cut(s) 162, 341, 687, 777, 990, 1003, 1131, 1170, 1177
FauI CCCGC 2 cut(s) 329, 335
FblI GTMKAC 2 cut(s) 270, 372
Fnu4HI GCNGC 6 cut(s) 210, 432, 827, 977, 1050, 1088
FokI GGATG 2 cut(s) 310, 332
FriOI GRGCYC 1 cut(s) 1078
Fsp4HI GCNGC 6 cut(s) 210, 432, 827, 977, 1050, 1088
FspBI CTAG 4 cut(s) 222, 764, 939, 971
GluI GCNGC 6 cut(s) 210, 432, 827, 977, 1050, 1088
GsaI CCCAGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 4 cut(s) 340, 516, 839, 950
HapII CCGG 5 cut(s) 26, 861, 954, 980, 1072
HgaI GACGC 2 cut(s) 38, 629
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 9 cut(s) 166, 345, 691, 781, 994, 1007, 1135, 1174, 1181
HincII GTYRAC 2 cut(s) 271, 373
HindII GTYRAC 2 cut(s) 271, 373
HinfI GANTC 7 cut(s) 86, 199, 377, 529, 562, 742, 857
HpaII CCGG 5 cut(s) 26, 861, 954, 980, 1072
HphI GGTGA 4 cut(s) 541, 641, 653, 1006
Hpy166II GTNNAC 5 cut(s) 44, 169, 271, 373, 417
Hpy188I TCNGA 4 cut(s) 113, 229, 657, 856
Hpy188III TCNNGA 7 cut(s) 80, 233, 627, 688, 739, 764, 809
Hpy8I GTNNAC 5 cut(s) 44, 169, 271, 373, 417
Hpy99I CGWCG 5 cut(s) 113, 272, 275, 377, 449
HpyAV CCTTC 3 cut(s) 400, 662, 1142
HpyCH4III ACNGT 1 cut(s) 1057
HpyCH4IV ACGT 3 cut(s) 40, 108, 444
HpyCH4V TGCA 4 cut(s) 323, 796, 815, 1003
HpyF10VI GCNNNNNNNGC 3 cut(s) 702, 1049, 1058
HpyF3I CTNAG 4 cut(s) 144, 197, 525, 544
HpySE526I ACGT 3 cut(s) 40, 108, 444
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 9 cut(s) 166, 345, 691, 781, 994, 1007, 1135, 1174, 1181
KroI GCCGGC 1 cut(s) 979
KroNI GCCGGC 1 cut(s) 981
Kzo9I GATC 8 cut(s) 4, 76, 229, 391, 495, 871, 915, 930
LmnI GCTCC 2 cut(s) 141, 1087
Lsp1109I GCAGC 4 cut(s) 221, 813, 963, 1036
LweI GCATC 8 cut(s) 310, 332, 576, 632, 802, 976, 990, 1152
MaeI CTAG 4 cut(s) 222, 764, 939, 971
MaeII ACGT 3 cut(s) 40, 108, 444
MaeIII GTNAC 2 cut(s) 301, 547
MalI GATC 8 cut(s) 6, 78, 231, 393, 497, 873, 917, 932
MboI GATC 8 cut(s) 4, 76, 229, 391, 495, 871, 915, 930
MboII GAAGA 7 cut(s) 95, 562, 737, 797, 872, 1115, 1157
MflI RGATCY 3 cut(s) 4, 76, 930
MhlI GDGCHC 2 cut(s) 1078, 1185
MluCI AATT 2 cut(s) 487, 908
MlyI GAGTC 5 cut(s) 208, 386, 571, 751, 866
MmeI TCCRAC 2 cut(s) 91, 483
MnlI CCTC 9 cut(s) 263, 287, 296, 460, 776, 837, 886, 940, 961
Mph1103I ATGCAT 2 cut(s) 325, 1005
MroNI GCCGGC 1 cut(s) 979
MseI TTAA 4 cut(s) 672, 732, 897, 927
MslI CAYNNNNRTG 1 cut(s) 995
MspA1I CMGCKG 3 cut(s) 212, 696, 1049
MspI CCGG 5 cut(s) 26, 861, 954, 980, 1072
MspR9I CCNGG 4 cut(s) 26, 493, 862, 1073
MvaI CCWGG 1 cut(s) 493
MwoI GCNNNNNNNGC 3 cut(s) 702, 1049, 1058
NaeI GCCGGC 1 cut(s) 981
NciI CCSGG 3 cut(s) 26, 862, 1073
NcoI CCATGG 1 cut(s) 990
NdeII GATC 8 cut(s) 4, 76, 229, 391, 495, 871, 915, 930
NgoMIV GCCGGC 1 cut(s) 979
NlaIII CATG 9 cut(s) 166, 345, 691, 781, 994, 1007, 1135, 1174, 1181
NlaIV GGNNCC 1 cut(s) 24
NmeAIII GCCGAG 1 cut(s) 865
NmuCI GTSAC 1 cut(s) 547
NsiI ATGCAT 2 cut(s) 325, 1005
NspI RCATGY 2 cut(s) 781, 1181
PagI TCATGA 1 cut(s) 687
PceI AGGCCT 1 cut(s) 950
PdiI GCCGGC 1 cut(s) 981
PfeI GAWTC 2 cut(s) 86, 529
PflMI CCANNNNNTGG 1 cut(s) 1196
PfoI TCCNGGA 2 cut(s) 24, 491
PkrI GCNGC 6 cut(s) 211, 433, 828, 978, 1051, 1089
PleI GAGTC 5 cut(s) 207, 385, 570, 750, 865
PpsI GAGTC 5 cut(s) 207, 385, 570, 750, 865
Psp6I CCWGG 1 cut(s) 491
PspFI CCCAGC 1 cut(s) 212
PspGI CCWGG 1 cut(s) 491
PspN4I GGNNCC 1 cut(s) 24
PspPI GGNCC 3 cut(s) 22, 235, 338
PsrI GAACNNNNNNTAC 2 cut(s) 152, 184
PstNI CAGNNNCTG 2 cut(s) 244, 1196
PsuI RGATCY 3 cut(s) 4, 76, 930
PvuII CAGCTG 2 cut(s) 212, 1049
RsaI GTAC 2 cut(s) 170, 614
RsaNI GTAC 2 cut(s) 169, 613
RseI CAYNNNNRTG 1 cut(s) 995
SalI GTCGAC 2 cut(s) 269, 371
SaqAI TTAA 4 cut(s) 672, 732, 897, 927
SatI GCNGC 6 cut(s) 210, 432, 827, 977, 1050, 1088
Sau3AI GATC 8 cut(s) 4, 76, 229, 391, 495, 871, 915, 930
Sau96I GGNCC 3 cut(s) 22, 235, 338
ScaI AGTACT 1 cut(s) 614
SchI GAGTC 5 cut(s) 208, 386, 571, 751, 866
ScrFI CCNGG 4 cut(s) 26, 493, 862, 1073
SduI GDGCHC 2 cut(s) 1078, 1185
SfaNI GCATC 8 cut(s) 310, 332, 576, 632, 802, 976, 990, 1152
SfcI CTRYAG 1 cut(s) 1044
SgrDI CGTCGACG 1 cut(s) 269
SinI GGWCC 2 cut(s) 22, 235
SmiMI CAYNNNNRTG 1 cut(s) 995
SmlI CTYRAG 2 cut(s) 254, 739
SmoI CTYRAG 2 cut(s) 254, 739
SpeI ACTAGT 1 cut(s) 221
Sse9I AATT 2 cut(s) 487, 908
SseBI AGGCCT 1 cut(s) 950
SsiI CCGC 9 cut(s) 317, 328, 336, 431, 539, 696, 904, 1085, 1088
SspMI CTAG 4 cut(s) 222, 764, 939, 971
StuI AGGCCT 1 cut(s) 950
StyD4I CCNGG 4 cut(s) 24, 491, 860, 1071
StyI CCWWGG 1 cut(s) 990
TaaI ACNGT 1 cut(s) 1057
TaiI ACGT 3 cut(s) 43, 111, 447
TaqI TCGA 4 cut(s) 81, 270, 372, 447
TasI AATT 2 cut(s) 487, 908
TatI WGTACW 2 cut(s) 168, 612
TauI GCSGC 2 cut(s) 434, 1090
TfiI GAWTC 2 cut(s) 86, 529
Tru1I TTAA 4 cut(s) 672, 732, 897, 927
Tru9I TTAA 4 cut(s) 672, 732, 897, 927
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 4 cut(s) 209, 826, 976, 1049
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 5 cut(s) 676, 1020, 1041, 1120, 1159
TspGWI ACGGA 1 cut(s) 256
TspRI CASTG 1 cut(s) 393
Van91I CCANNNNNTGG 1 cut(s) 1196
VpaK11BI GGWCC 2 cut(s) 22, 235
XapI RAATTY 1 cut(s) 487
XbaI TCTAGA 1 cut(s) 763
XceI RCATGY 2 cut(s) 781, 1181
XmiI GTMKAC 2 cut(s) 270, 372
XspI CTAG 4 cut(s) 222, 764, 939, 971
ZraI GACGTC 1 cut(s) 109
ZrmI AGTACT 1 cut(s) 614
Zsp2I ATGCAT 2 cut(s) 325, 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.