Rroxscaffold_3G00250320

Belongs to the serpin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000163
Physical Location & Seq
Reverse (-)
44119673 .. 44132402
12730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00250320.1

Sequence Viewer

Length: 1869 bp
ATGGAAAACAAAGAAGAGACGAATGAGCAATGCTACCCATTATGCCCTCCACAACCATTTGAATATCATACTCCGTCGTCTTCCTCCAGGTATTATTCAGCAGCCGATTATCACCGACCATCATCGCCACCGAAGCCTTCCTTCAAACCATCAAGGGAACTCGAAGAATCCATCAAAAATCAAACTGACGTTACACTGAGATTCACAAAGCAACTGCTTCTGACTTTCGGCAAGAACAATAACATGGTCTACTCCCCACTGTCCATCCACGTGGTTCTTAGCATGATAGTGGCTGGGACAAAGGGCCACATTCAGGAGAAGTTGCTCCGTTTCCTCAAGTCCAAGTCCATCGACGAGCTCAATGATCTGGCCTCCAATGTCGTCCCATTGGTCTTTGCTGATGGATCCTCAAGTGGTGGGCCTCGCTTGTCATTCGCCAATGGCGTTTGGGTTGAGAAGTCTAGCCCTATAAAGCTTTCTTTCAAAGAAGTAATGGACACTACTTACAAGGCGTCATTGAACCAAGTAGATTTTAATACCAAGCCTGAAGAAGTGCGATGTGAAGTGAATTTATGGGCGGAGAAAGAGACCAATGGCCGTGTCAAAGAGATTATTCCTGAAGGGTTAGTTACCAGCGTTACAAAGCTCATCCTTGCGAATGCATTATACTTCAAAGGAGCTTGGGATCGGGAGTTTAATGCATCAAAAACACAATGGATAAATTTTCACCTTCTGAATGGGAGCTCAATTAGAGCACCCTTCATGACCAGCTTGGAGGATCATTATATTAGTGCCTTTGACGATCATCGTCCGGATAATAAAAACTCAAAAAAAGTCTTAAAGCTCCCCTACAAACAAGGTGGTGATTACGAGAGACGTTTCTCCATGTTGGTGTTTTTTCCAAATAAATGGACTGGACTGCAAGCGTTGGTTGAGAGATTTTCTTCCGAGTCTGGGTTTATAGATCGATACATTCCTCACCGAGAAGTTTCAGTTGGTAGATTTTTTATCCCCAAGTTTAAAATATCTGCTCGCTTTGATGCTCTTGATGTTCTGAAACCGTTAGGATTATCTCTTAAAGATGGAGACTTCACAGAGATGGCGGAGGATATGAACCTAAGTTTAAGCAATATATTCCATAAATCCTTCATTGAAGTTAATGAAGAAGGCACAGAAGCTGCTGCTGTGACTGCTGCCCTTTTTTGTGGTCGTGCGATGGCACCCAGAATTAAGATGGATTCTGTGGCAGATCACCCATTTCTTTTTCTCATAAGAGATGAATTGACTAGAACAGTTATGTTCATTGGGCATAGAGAATCAGAAAGAGGAGAAATATTGAGAAACCTATCGAGCGATAGATATCGAGATTTTTTGTTCTCTTGCAAAGTAGTTGGCTTTTGGGAAGGTTGGCGTGCAATGGACAACAAAGATGATGATCACCCTGCAGGACAATATGAGCAATCTTTCTATACTCCAAGTATTCCACATTATACTGCTTCTCCAATCTACATTCCACAACCATATCGCCCTACACAACAATATGAACATCCTCGATATTCTCCTAGCTCTCCGTCCAACATTCCACAACCATATCACCCTAAACGACAATATGAACACCCACGGTATTCTCCTTTCCCTCCATCATACTCTCCATGTTCTCCTTCCTCTCCACCATACTTTTCACAGCGATATCACCCTACAATACAATTTGAACTCCATAGGTATTCTCCTGGTAGAGATCCTTCCCCTGGCGGGGGTCATGTGACCAGTAGTTATCCACCGCCTTCCTTCAAACCATCTAGGGAACTCCGAGAATCCATTAAAAACCAAACCGATGTTGCACCGGAAATCACAAAGCAACGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05146 ko05164 map03013 map05146 map05164
Pfam Domains
Protein Families
Serpin

Protein Analysis

622

Amino Acids

71.24

Weight (kDa)

8.27

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 60 - 437 3.4e-83 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64020
fragaria_vesca FvH4_1g24600 FvH4_1g24640 FvH4_2g15000 FvH4_3g03700 FvH4_3g03710 FvH4_3g09790 FvH4_3g09790 FvH4_3g09810 FvH4_3g09811 FvH4_3g23720 FvH4_3g32870 FvH4_3g32890 FvH4_3g37550 FvH4_3g37560 FvH4_5g01890 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g21100 FvH4_5g21110 FvH4_5g21330 FvH4_5g21331 FvH4_5g21520 FvH4_5g21540 FvH4_5g21970 FvH4_6g01280
malus_domestica MD00G1084800.v1.1 MD00G1141300.v1.1 MD02G1102800.v1.1 MD02G1282200.v1.1 MD02G1282300.v1.1 MD03G1207900.v1.1 MD03G1230000.v1.1 MD04G1136400.v1.1 MD04G1136500.v1.1 MD04G1189000.v1.1 MD05G1248700.v1.1 MD05G1248800.v1.1 MD06G1030400.v1.1 MD10G1229200.v1.1 MD10G1229300.v1.1 MD15G1291400.v1.1 MD15G1431300.v1.1
prunus_persica Prupe.5G041800_v2.0.a1 Prupe.5G041900_v2.0.a1 Prupe.5G042000_v2.0.a1 Prupe.5G042100_v2.0.a1 Prupe.5G042200_v2.0.a1
pyrus_communis pycom02g14630 pycom02g24090 pycom04g12410 pycom04g16760 pycom05g22550 pycom10g19280 pycom12g05670 pycom15g25490 pycom15g38110
rosa_chinensis RchiOBHm_Chr2g0122001 RchiOBHm_Chr2g0163171 RchiOBHm_Chr2g0163181 RchiOBHm_Chr3g0455201 RchiOBHm_Chr5g0005751 RchiOBHm_Chr5g0015501 RchiOBHm_Chr5g0015511 RchiOBHm_Chr5g0015521 RchiOBHm_Chr5g0015531 RchiOBHm_Chr5g0060021 RchiOBHm_Chr7g0187971 RchiOBHm_Chr7g0207951 RchiOBHm_Chr7g0208671 RchiOBHm_Chr7g0208691 RchiOBHm_Chr7g0208941 RchiOBHm_Chr7g0208951 RchiOBHm_Chr7g0208961
rosa_laevigata RLG00000003132 RLG00000003177 RLG00000003178 RLG00000003179 RLG00000003180 RLG00000003201 RLG00000003202 RLG00000003270 RLG00000004739 RLG00000004741 RLG00000004744 RLG00000021379 RLG00000025378 RLG00000031323 RLG00000031324 RLG00000032205 RLG00000032206 RLG00000035339
rosa_multiflora Rmu_co8189380.1_g000001 Rmu_co8284591.1_g000001 Rmu_co8360283.1_g000002 Rmu_co8379053.1_g000001 Rmu_sc0000493.1_g000007 Rmu_sc0000532.1_g000030 Rmu_sc0000532.1_g000031 Rmu_sc0000570.1_g000033 Rmu_sc0000905.1_g000012 Rmu_sc0001730.1_g000002 Rmu_sc0002045.1_g000039 Rmu_sc0003064.1_g000001 Rmu_sc0003064.1_g000002 Rmu_sc0003064.1_g000006 Rmu_sc0003880.1_g000012 Rmu_sc0004103.1_g000003 Rmu_sc0005082.1_g000007 Rmu_sc0011272.1_g000002 Rmu_sc0015523.1_g000021 Rmu_sc0015523.1_g000022 Rmu_sc0019960.1_g000007 Rmu_sc0029514.1_g000001 Rmu_ssc0000422.1_g000010
rosa_roxburghii Rroxscaffold_1G00013660 Rroxscaffold_1G00060980 Rroxscaffold_1G00060990 Rroxscaffold_1G00061000 Rroxscaffold_1G00061010 Rroxscaffold_2G00087490 Rroxscaffold_2G00122080 Rroxscaffold_2G00123490 Rroxscaffold_3G00249930 Rroxscaffold_3G00249940 Rroxscaffold_3G00249950 Rroxscaffold_3G00249980 Rroxscaffold_3G00250310 Rroxscaffold_3G00250320 Rroxscaffold_3G00266850 Rroxscaffold_3G00266870 Rroxscaffold_3G00266910 Rroxscaffold_6G00424480
rosa_rugosa Rorug02G0228900 Rorug02G0236000 Rorug02G0501100 Rorug03G0004700 Rorug03G0004700 Rorug03G0004800 Rorug04G0417800 Rorug05G0027800 Rorug05G0027900 Rorug05G0028000 Rorug05G0327700 Rorug05G0384800 Rorug06G0486000 Rorug06G0486100 Rorug06G0486200 Rorug06G0486200 Rorug06G0486300 Rorug06G0486400 Rorug06G0486500 Rorug07G0100400 Rorug07G0105400 Rorug07G0105400 Rorug07G0107300 Rorug07G0107400 Rorug07G0107500 Rorug07G0113300
rosa_samantha Rh7AG091300 Rh7BG092800 Rh7CG091600 Rh7CG257500 Rh7DG093500
rosa_wichuraiana Rw0G001260 Rw0G017680 Rw2G023470 Rw3G005130 Rw5G004560 Rw5G010480 Rw7G020400 Rw7G020410 Rw7G020540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1219
AccI GTMKAC 1 cut(s) 249
AccIII TCCGGA 1 cut(s) 811
AciI CCGC 4 cut(s) 578, 1103, 1752, 1781
AclWI GGATC 5 cut(s) 399, 412, 693, 786, 1733
AcoI YGGCCR 1 cut(s) 595
AcsI RAATTY 2 cut(s) 568, 721
AcuI CTGAAG 2 cut(s) 567, 639
AcvI CACGTG 1 cut(s) 271
AcyI GRCGYC 1 cut(s) 512
AfiI CCNNNNNNNGG 6 cut(s) 313, 954, 1748, 1752, 1753, 1754
AgsI TTSAA 8 cut(s) 62, 145, 484, 520, 673, 1154, 1712, 1792
AjnI CCWGG 3 cut(s) 86, 1729, 1747
AjuI GAANNNNNNNTTGG 2 cut(s) 978, 1010
AluBI AGCT 9 cut(s) 358, 475, 646, 680, 744, 771, 844, 1178, 1566
AluI AGCT 9 cut(s) 358, 475, 646, 680, 744, 771, 844, 1178, 1566
Alw21I GWGCWC 3 cut(s) 360, 746, 757
Alw26I GTCTC 4 cut(s) 11, 581, 868, 1080
AlwI GGATC 5 cut(s) 399, 412, 693, 786, 1733
AlwNI CAGNNNCTG 1 cut(s) 1178
Aor13HI TCCGGA 1 cut(s) 811
AoxI GGCC 4 cut(s) 304, 369, 419, 595
ApeKI GCWGC 4 cut(s) 101, 1178, 1181, 1193
ApoI RAATTY 2 cut(s) 568, 721
AspS9I GGNCC 2 cut(s) 304, 419
AsuHPI GGTGA 8 cut(s) 104, 719, 875, 971, 1244, 1430, 1586, 1685
BamHI GGATCC 1 cut(s) 404
BanI GGYRCC 1 cut(s) 1219
BanII GRGCYC 2 cut(s) 360, 746
BarI GAAGNNNNNNTAC 2 cut(s) 1726, 1758
BbrPI CACGTG 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 3 cut(s) 360, 746, 757
BbvI GCAGC 4 cut(s) 113, 1165, 1168, 1180
BceAI ACGGC 1 cut(s) 582
BciT130I CCWGG 3 cut(s) 88, 1731, 1749
BclI TGATCA 1 cut(s) 1435
BcoDI GTCTC 4 cut(s) 11, 581, 868, 1080
BfaI CTAG 4 cut(s) 462, 1287, 1563, 1800
BfmI CTRYAG 1 cut(s) 1443
BisI GCNGC 4 cut(s) 102, 1179, 1182, 1194
BlsI GCNGC 4 cut(s) 103, 1180, 1183, 1195
Bme1390I CCNGG 3 cut(s) 88, 1731, 1749
BmgT120I GGNCC 2 cut(s) 304, 419
BmiI GGNNCC 2 cut(s) 406, 1221
BmrFI CCNGG 3 cut(s) 88, 1731, 1749
BmsI GCATC 2 cut(s) 710, 1030
BpiI GAAGAC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 70
BpuEI CTTGAG 2 cut(s) 320, 394
Bsa29I ATCGAT 1 cut(s) 967
BsaAI YACGTR 1 cut(s) 271
BsaBI GATNNNNATC 2 cut(s) 1359, 1434
BsaHI GRCGYC 1 cut(s) 512
BsaI GGTCTC 1 cut(s) 581
BsaJI CCNNGG 2 cut(s) 1619, 1747
BsaWI WCCGGW 2 cut(s) 811, 1843
BsaXI ACNNNNNCTCC 2 cut(s) 1483, 1513
Bsc4I CCNNNNNNNGG 6 cut(s) 313, 954, 1748, 1752, 1753, 1754
Bse1I ACTGG 2 cut(s) 919, 1767
Bse3DI GCAATG 2 cut(s) 35, 1422
Bse8I GATNNNNATC 2 cut(s) 1359, 1434
BseAI TCCGGA 1 cut(s) 811
BseBI CCWGG 3 cut(s) 88, 1731, 1749
BseCI ATCGAT 1 cut(s) 967
BseDI CCNNGG 2 cut(s) 1619, 1747
BseGI GGATG 3 cut(s) 264, 648, 1546
BseJI GATNNNNATC 2 cut(s) 1359, 1434
BseLI CCNNNNNNNGG 6 cut(s) 313, 954, 1748, 1752, 1753, 1754
BseMI GCAATG 2 cut(s) 35, 1422
BseMII CTCAG 1 cut(s) 188
BseNI ACTGG 2 cut(s) 919, 1767
BseRI GAGGAG 1 cut(s) 1341
BseXI GCAGC 4 cut(s) 113, 1165, 1168, 1180
BseYI CCCAGC 1 cut(s) 293
BshFI GGCC 4 cut(s) 306, 371, 421, 597
BshNI GGYRCC 1 cut(s) 1219
BshVI ATCGAT 1 cut(s) 967
BsiHKAI GWGCWC 3 cut(s) 360, 746, 757
BsiSI CCGG 2 cut(s) 812, 1844
BslFI GGGAC 2 cut(s) 310, 368
BslI CCNNNNNNNGG 6 cut(s) 313, 954, 1748, 1752, 1753, 1754
BsmAI GTCTC 4 cut(s) 11, 581, 868, 1080
BsmBI CGTCTC 2 cut(s) 11, 868
BsmFI GGGAC 2 cut(s) 310, 368
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 4 cut(s) 306, 371, 421, 597
Bso31I GGTCTC 1 cut(s) 581
Bsp1286I GDGCHC 3 cut(s) 360, 746, 757
Bsp13I TCCGGA 1 cut(s) 811
Bsp143I GATC 9 cut(s) 364, 404, 685, 778, 802, 964, 1249, 1435, 1738
BspACI CCGC 4 cut(s) 578, 1103, 1752, 1781
BspANI GGCC 4 cut(s) 306, 371, 421, 597
BspCNI CTCAG 1 cut(s) 189
BspDI ATCGAT 1 cut(s) 967
BspEI TCCGGA 1 cut(s) 811
BspHI TCATGA 1 cut(s) 762
BspLI GGNNCC 2 cut(s) 406, 1221
BspMAI CTGCAG 1 cut(s) 1447
BspPI GGATC 5 cut(s) 399, 412, 693, 786, 1733
BspT107I GGYRCC 1 cut(s) 1219
BspTNI GGTCTC 1 cut(s) 581
BsrDI GCAATG 2 cut(s) 35, 1422
BsrI ACTGG 2 cut(s) 919, 1767
BssECI CCNNGG 2 cut(s) 1619, 1747
BssMI GATC 9 cut(s) 364, 404, 685, 778, 802, 964, 1249, 1435, 1738
BssNI GRCGYC 1 cut(s) 512
Bst2UI CCWGG 3 cut(s) 88, 1731, 1749
Bst4CI ACNGT 4 cut(s) 261, 1062, 1294, 1623
Bst6I CTCTTC 1 cut(s) 9
BstACI GRCGYC 1 cut(s) 512
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 3 cut(s) 924, 1033, 1413
BstDEI CTNAG 3 cut(s) 197, 278, 1118
BstDSI CCRYGG 1 cut(s) 1619
BstF5I GGATG 3 cut(s) 264, 648, 1546
BstKTI GATC 9 cut(s) 367, 407, 688, 781, 805, 967, 1252, 1438, 1741
BstMAI GTCTC 4 cut(s) 11, 581, 868, 1080
BstMBI GATC 9 cut(s) 364, 404, 685, 778, 802, 964, 1249, 1435, 1738
BstMWI GCNNNNNNNGC 2 cut(s) 133, 1190
BstNI CCWGG 3 cut(s) 88, 1731, 1749
BstSCI CCNGG 3 cut(s) 86, 1729, 1747
BstSFI CTRYAG 1 cut(s) 1443
BstV1I GCAGC 4 cut(s) 113, 1165, 1168, 1180
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 2 cut(s) 404, 1738
BstXI CCANNNNNNTGG 2 cut(s) 271, 909
BstYI RGATCY 2 cut(s) 404, 1738
Bsu15I ATCGAT 1 cut(s) 967
BsuRI GGCC 4 cut(s) 306, 371, 421, 597
BsuTUI ATCGAT 1 cut(s) 967
BtgI CCRYGG 1 cut(s) 1619
BtgZI GCGATG 3 cut(s) 108, 571, 1229
BtsCI GGATG 3 cut(s) 264, 648, 1546
BtsIMutI CAGTG 2 cut(s) 194, 257
Cac8I GCNNGC 3 cut(s) 924, 1033, 1413
CaiI CAGNNNCTG 1 cut(s) 1178
CciI TCATGA 1 cut(s) 762
Cfr13I GGNCC 2 cut(s) 304, 419
ClaI ATCGAT 1 cut(s) 967
CseI GACGC 1 cut(s) 501
CspCI CAANNNNNGTGG 2 cut(s) 841, 876
CviAII CATG 6 cut(s) 244, 283, 763, 886, 1653, 1760
DdeI CTNAG 3 cut(s) 197, 278, 1118
DpnI GATC 9 cut(s) 366, 406, 687, 780, 804, 966, 1251, 1437, 1740
DpnII GATC 9 cut(s) 364, 404, 685, 778, 802, 964, 1249, 1435, 1738
DraI TTTAAA 1 cut(s) 1021
EaeI YGGCCR 1 cut(s) 595
Eam1104I CTCTTC 1 cut(s) 9
EarI CTCTTC 1 cut(s) 9
EciI GGCGGA 2 cut(s) 593, 1118
Ecl136II GAGCTC 2 cut(s) 358, 744
Eco24I GRGCYC 2 cut(s) 360, 746
Eco31I GGTCTC 1 cut(s) 581
Eco32I GATATC 2 cut(s) 1361, 1691
Eco53kI GAGCTC 2 cut(s) 358, 744
Eco57I CTGAAG 2 cut(s) 567, 639
Eco72I CACGTG 1 cut(s) 271
EcoICRI GAGCTC 2 cut(s) 358, 744
EcoRII CCWGG 3 cut(s) 86, 1729, 1747
EcoRV GATATC 2 cut(s) 1361, 1691
EcoT22I ATGCAT 2 cut(s) 664, 703
EcoT38I GRGCYC 2 cut(s) 360, 746
Esp3I CGTCTC 2 cut(s) 11, 868
FaeI CATG 6 cut(s) 247, 286, 766, 889, 1656, 1763
FalI AAGNNNNNCTT 3 cut(s) 125, 157, 1847
FaqI GGGAC 2 cut(s) 310, 368
FatI CATG 6 cut(s) 243, 282, 762, 885, 1652, 1759
FauI CCCGC 1 cut(s) 1745
FbaI TGATCA 1 cut(s) 1435
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 4 cut(s) 102, 1179, 1182, 1194
FokI GGATG 3 cut(s) 251, 635, 1533
FriOI GRGCYC 2 cut(s) 360, 746
Fsp4HI GCNGC 4 cut(s) 102, 1179, 1182, 1194
FspBI CTAG 4 cut(s) 462, 1287, 1563, 1800
GluI GCNGC 4 cut(s) 102, 1179, 1182, 1194
GsaI CCCAGC 1 cut(s) 297
GsuI CTGGAG 1 cut(s) 70
HaeIII GGCC 4 cut(s) 306, 371, 421, 597
HapII CCGG 2 cut(s) 812, 1844
HgaI GACGC 1 cut(s) 501
Hin1I GRCGYC 1 cut(s) 512
Hin1II CATG 6 cut(s) 247, 286, 766, 889, 1656, 1763
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 6 cut(s) 167, 201, 950, 1238, 1316, 1814
HpaII CCGG 2 cut(s) 812, 1844
HphI GGTGA 8 cut(s) 104, 719, 875, 971, 1244, 1430, 1586, 1685
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 6 cut(s) 222, 735, 949, 1056, 1321, 1811
Hpy188III TCNNGA 7 cut(s) 314, 617, 689, 763, 812, 1046, 1364
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 2 cut(s) 79, 356
HpyCH4III ACNGT 4 cut(s) 261, 1062, 1294, 1623
HpyCH4IV ACGT 3 cut(s) 189, 270, 877
HpyCH4V TGCA 7 cut(s) 662, 701, 922, 1383, 1415, 1445, 1841
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 1190
HpyF3I CTNAG 3 cut(s) 197, 278, 1118
HpySE526I ACGT 3 cut(s) 189, 270, 877
Hsp92I GRCGYC 1 cut(s) 512
Hsp92II CATG 6 cut(s) 247, 286, 766, 889, 1656, 1763
Kpn2I TCCGGA 1 cut(s) 811
Ksp22I TGATCA 1 cut(s) 1435
Kzo9I GATC 9 cut(s) 364, 404, 685, 778, 802, 964, 1249, 1435, 1738
LmnI GCTCC 4 cut(s) 330, 677, 741, 849
Lsp1109I GCAGC 4 cut(s) 113, 1165, 1168, 1180
LweI GCATC 2 cut(s) 710, 1030
MaeI CTAG 4 cut(s) 462, 1287, 1563, 1800
MaeII ACGT 3 cut(s) 189, 270, 877
MaeIII GTNAC 5 cut(s) 190, 628, 637, 1186, 1762
MalI GATC 9 cut(s) 366, 406, 687, 780, 804, 966, 1251, 1437, 1740
MboI GATC 9 cut(s) 364, 404, 685, 778, 802, 964, 1249, 1435, 1738
MboII GAAGA 6 cut(s) 26, 72, 176, 560, 936, 1175
MflI RGATCY 2 cut(s) 404, 1738
MhlI GDGCHC 3 cut(s) 360, 746, 757
MluCI AATT 6 cut(s) 568, 721, 747, 1227, 1280, 1706
MlyI GAGTC 1 cut(s) 959
MmeI TCCRAC 1 cut(s) 1599
Mph1103I ATGCAT 2 cut(s) 664, 703
MroI TCCGGA 1 cut(s) 811
MseI TTAA 9 cut(s) 534, 696, 839, 1020, 1077, 1124, 1158, 1230, 1821
MslI CAYNNNNRTG 4 cut(s) 269, 287, 890, 1097
MspI CCGG 2 cut(s) 812, 1844
MspR9I CCNGG 3 cut(s) 88, 1731, 1749
Mva1269I GAATGC 1 cut(s) 664
MvaI CCWGG 3 cut(s) 88, 1731, 1749
MwoI GCNNNNNNNGC 2 cut(s) 133, 1190
NdeII GATC 9 cut(s) 364, 404, 685, 778, 802, 964, 1249, 1435, 1738
NlaIII CATG 6 cut(s) 247, 286, 766, 889, 1656, 1763
NlaIV GGNNCC 2 cut(s) 406, 1221
NmuCI GTSAC 2 cut(s) 1186, 1762
NsiI ATGCAT 2 cut(s) 664, 703
PagI TCATGA 1 cut(s) 762
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PctI GAATGC 1 cut(s) 664
PfeI GAWTC 5 cut(s) 167, 201, 1238, 1316, 1814
PkrI GCNGC 4 cut(s) 103, 1180, 1183, 1195
PleI GAGTC 1 cut(s) 958
PmaCI CACGTG 1 cut(s) 271
PmlI CACGTG 1 cut(s) 271
PpsI GAGTC 1 cut(s) 958
Ppu21I YACGTR 1 cut(s) 271
Psp124BI GAGCTC 2 cut(s) 360, 746
Psp6I CCWGG 3 cut(s) 86, 1729, 1747
PspCI CACGTG 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 3 cut(s) 86, 1729, 1747
PspN4I GGNNCC 2 cut(s) 406, 1221
PspPI GGNCC 2 cut(s) 304, 419
PstI CTGCAG 1 cut(s) 1447
PstNI CAGNNNCTG 1 cut(s) 1178
PsuI RGATCY 2 cut(s) 404, 1738
RseI CAYNNNNRTG 4 cut(s) 269, 287, 890, 1097
SacI GAGCTC 2 cut(s) 360, 746
SaqAI TTAA 9 cut(s) 534, 696, 839, 1020, 1077, 1124, 1158, 1230, 1821
SatI GCNGC 4 cut(s) 102, 1179, 1182, 1194
Sau3AI GATC 9 cut(s) 364, 404, 685, 778, 802, 964, 1249, 1435, 1738
Sau96I GGNCC 2 cut(s) 304, 419
SbfI CCTGCAGG 1 cut(s) 1447
SchI GAGTC 1 cut(s) 959
ScrFI CCNGG 3 cut(s) 88, 1731, 1749
SdaI CCTGCAGG 1 cut(s) 1447
SduI GDGCHC 3 cut(s) 360, 746, 757
SfaNI GCATC 2 cut(s) 710, 1030
SfcI CTRYAG 1 cut(s) 1443
SmiMI CAYNNNNRTG 4 cut(s) 269, 287, 890, 1097
SmlI CTYRAG 2 cut(s) 335, 409
SmoI CTYRAG 2 cut(s) 335, 409
Sse8387I CCTGCAGG 1 cut(s) 1447
Sse9I AATT 6 cut(s) 568, 721, 747, 1227, 1280, 1706
SsiI CCGC 4 cut(s) 578, 1103, 1752, 1781
SspI AATATT 1 cut(s) 1335
SspMI CTAG 4 cut(s) 462, 1287, 1563, 1800
SstI GAGCTC 2 cut(s) 360, 746
StyD4I CCNGG 3 cut(s) 86, 1729, 1747
TaaI ACNGT 4 cut(s) 261, 1062, 1294, 1623
TaiI ACGT 3 cut(s) 192, 273, 880
TaqI TCGA 6 cut(s) 162, 351, 967, 1349, 1363, 1552
TasI AATT 6 cut(s) 568, 721, 747, 1227, 1280, 1706
TfiI GAWTC 5 cut(s) 167, 201, 1238, 1316, 1814
Tru1I TTAA 9 cut(s) 534, 696, 839, 1020, 1077, 1124, 1158, 1230, 1821
Tru9I TTAA 9 cut(s) 534, 696, 839, 1020, 1077, 1124, 1158, 1230, 1821
TscAI CASTG 2 cut(s) 201, 264
TseFI GTSAC 2 cut(s) 1186, 1762
TseI GCWGC 4 cut(s) 101, 1178, 1181, 1193
Tsp45I GTSAC 2 cut(s) 1186, 1762
TspDTI ATGAA 8 cut(s) 751, 1127, 1138, 1176, 1291, 1293, 1557, 1626
TspGWI ACGGA 3 cut(s) 63, 317, 1560
TspRI CASTG 2 cut(s) 201, 264
XapI RAATTY 2 cut(s) 568, 721
XcmI CCANNNNNNNNNTGG 1 cut(s) 1231
XmiI GTMKAC 1 cut(s) 249
XspI CTAG 4 cut(s) 462, 1287, 1563, 1800
Zsp2I ATGCAT 2 cut(s) 664, 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.