Rroxscaffold_3G00249930

Belongs to the serpin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000163
Physical Location & Seq
Reverse (-)
43695386 .. 43697126
1741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00249930.1

Sequence Viewer

Length: 1230 bp
ATGGCGGCAACCCACTCCCTCCTCGGCCGTTCACAGGCCCTCCGTCCAAATTCTTCCGGCCACCGTCTGCTGGTTTCCTTTCTCATTCTCTTTCTCACTTTCGTCGTCGTATGTATTTCTCTCTTTTGTCCTCTTTCTGATCTGCGTTACCAGAGCCCAAACTCCGATCCTAACACTTTCAACGTCGACGACCATATCAATCCGATCTCGTGGAGTTCAATGAACCAGACCCAGAAAGCCAGCAAACAGACCGAGGTGGCTTTTACTATTATGAAGCAGCTGTTTTTGAGTACTGAAGCCAAGAACAAAAATATGGTGTACTCGCCGCTATCCATCCACATGGTTCTCAGCCTATTAGCGGCTGAGTCAAACGGCCCTACACAGGATCAGTTGCTCACCTTCCTCAAGTCCAAGTCCACCCAAGAGCTCAACTATCTGGCCTCCACTCTCATGCCTCTGGCTACTTTAAAGCAAGTCGATTTCAAGGGGAAGTCAGATGAAGTTAGAATGGAAGTGAATTCATGGGCCGAGAAGGAGACTAATGGTTGCATCAAAGATATTCTTCCTTCTGGCTCAGTCAACAGCCTAACAAGGCTCATTCTCGCAAATGCATTATACTTCAAAGGAGATTGGAAGGACAGATTTGATGCATCAGGAACAAAAGAGTATGATTTCCACCTTCTCAATGGGAACTCAGTTAAGGCTCCTTTCATGACGAAGTGGATAACAAGTGGAAGGCGGTATATAAGTGTTTTTGACAGTTTCAAAGTCTTCAAACTCCCTTACAAACAAGGTCAAGATCGTGAGAAGTGTTTCTCCATGTATGTGTTTCTTCCAAATGAAAGGTATGGGTTGCCCGCTTTAGTTGAGAGATTTTCTTCTGAGTCTGGGTTCTTAGATCGCCATCTTCCGGATGAAACAGTTGAAGTTTGTGCCTTTTTAATGCCAAAGTTTAAGTTTTCTTCTAGTTTTGAAGCTTCCACGGTTCTGAAAACTTTAGGATTGGAGTTGCCTTTTGTTTCCAGTATACAACATCGATCATTCATTGATGTTAATGATGATGGTACAGAAGCTGCTGCTGTTACGGTTGCTCTTCTAACGGGTTCCTCTGGGATTCAGGAGAAGACGATAGACTTTGTGGCAGATCATCCCTTCCTTTTTCTTATTAGAGAAGAAACCACTGGATCGGTGCTGTTCATTGGGCAAGTCCTCCATCTGATCGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05146 ko05164 map03013 map05146 map05164
Pfam Domains
Protein Families
Serpin

Protein Analysis

409

Amino Acids

45.89

Weight (kDa)

6.17

Isoelectric Point (pI)

35.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 82 - 142 5.9e-09 Serpin (serine protease inhibitor)
Serpin PF00079 154 - 405 1.5e-58 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64020
fragaria_vesca FvH4_1g24600 FvH4_1g24640 FvH4_2g15000 FvH4_3g03700 FvH4_3g03710 FvH4_3g09790 FvH4_3g09790 FvH4_3g09810 FvH4_3g09811 FvH4_3g23720 FvH4_3g32870 FvH4_3g32890 FvH4_3g37550 FvH4_3g37560 FvH4_5g01890 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g10280 FvH4_5g21100 FvH4_5g21110 FvH4_5g21330 FvH4_5g21331 FvH4_5g21520 FvH4_5g21540 FvH4_5g21970 FvH4_6g01280
malus_domestica MD00G1084800.v1.1 MD00G1141300.v1.1 MD02G1102800.v1.1 MD02G1282200.v1.1 MD02G1282300.v1.1 MD03G1207900.v1.1 MD03G1230000.v1.1 MD04G1136400.v1.1 MD04G1136500.v1.1 MD04G1189000.v1.1 MD05G1248700.v1.1 MD05G1248800.v1.1 MD06G1030400.v1.1 MD10G1229200.v1.1 MD10G1229300.v1.1 MD15G1291400.v1.1 MD15G1431300.v1.1
prunus_persica Prupe.5G041800_v2.0.a1 Prupe.5G041900_v2.0.a1 Prupe.5G042000_v2.0.a1 Prupe.5G042100_v2.0.a1 Prupe.5G042200_v2.0.a1
pyrus_communis pycom02g14630 pycom02g24090 pycom04g12410 pycom04g16760 pycom05g22550 pycom10g19280 pycom12g05670 pycom15g25490 pycom15g38110
rosa_chinensis RchiOBHm_Chr2g0122001 RchiOBHm_Chr2g0163171 RchiOBHm_Chr2g0163181 RchiOBHm_Chr3g0455201 RchiOBHm_Chr5g0005751 RchiOBHm_Chr5g0015501 RchiOBHm_Chr5g0015511 RchiOBHm_Chr5g0015521 RchiOBHm_Chr5g0015531 RchiOBHm_Chr5g0060021 RchiOBHm_Chr7g0187971 RchiOBHm_Chr7g0207951 RchiOBHm_Chr7g0208671 RchiOBHm_Chr7g0208691 RchiOBHm_Chr7g0208941 RchiOBHm_Chr7g0208951 RchiOBHm_Chr7g0208961
rosa_laevigata RLG00000003132 RLG00000003177 RLG00000003178 RLG00000003179 RLG00000003180 RLG00000003201 RLG00000003202 RLG00000003270 RLG00000004739 RLG00000004741 RLG00000004744 RLG00000021379 RLG00000025378 RLG00000031323 RLG00000031324 RLG00000032205 RLG00000032206 RLG00000035339
rosa_multiflora Rmu_co8189380.1_g000001 Rmu_co8284591.1_g000001 Rmu_co8360283.1_g000002 Rmu_co8379053.1_g000001 Rmu_sc0000493.1_g000007 Rmu_sc0000532.1_g000030 Rmu_sc0000532.1_g000031 Rmu_sc0000570.1_g000033 Rmu_sc0000905.1_g000012 Rmu_sc0001730.1_g000002 Rmu_sc0002045.1_g000039 Rmu_sc0003064.1_g000001 Rmu_sc0003064.1_g000002 Rmu_sc0003064.1_g000006 Rmu_sc0003880.1_g000012 Rmu_sc0004103.1_g000003 Rmu_sc0005082.1_g000007 Rmu_sc0011272.1_g000002 Rmu_sc0015523.1_g000021 Rmu_sc0015523.1_g000022 Rmu_sc0019960.1_g000007 Rmu_sc0029514.1_g000001 Rmu_ssc0000422.1_g000010
rosa_roxburghii Rroxscaffold_1G00013660 Rroxscaffold_1G00060980 Rroxscaffold_1G00060990 Rroxscaffold_1G00061000 Rroxscaffold_1G00061010 Rroxscaffold_2G00087490 Rroxscaffold_2G00122080 Rroxscaffold_2G00123490 Rroxscaffold_3G00249930 Rroxscaffold_3G00249940 Rroxscaffold_3G00249950 Rroxscaffold_3G00249980 Rroxscaffold_3G00250310 Rroxscaffold_3G00250320 Rroxscaffold_3G00266850 Rroxscaffold_3G00266870 Rroxscaffold_3G00266910 Rroxscaffold_6G00424480
rosa_rugosa Rorug02G0228900 Rorug02G0236000 Rorug02G0501100 Rorug03G0004700 Rorug03G0004700 Rorug03G0004800 Rorug04G0417800 Rorug05G0027800 Rorug05G0027900 Rorug05G0028000 Rorug05G0327700 Rorug05G0384800 Rorug06G0486000 Rorug06G0486100 Rorug06G0486200 Rorug06G0486200 Rorug06G0486300 Rorug06G0486400 Rorug06G0486500 Rorug07G0100400 Rorug07G0105400 Rorug07G0105400 Rorug07G0107300 Rorug07G0107400 Rorug07G0107500 Rorug07G0113300
rosa_samantha Rh7AG091300 Rh7BG092800 Rh7CG091600 Rh7CG257500 Rh7DG093500
rosa_wichuraiana Rw0G001260 Rw0G017680 Rw2G023470 Rw3G005130 Rw5G004560 Rw5G010480 Rw7G020400 Rw7G020410 Rw7G020540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 186, 1027
AccIII TCCGGA 1 cut(s) 910
AciI CCGC 5 cut(s) 5, 326, 359, 739, 858
AclWI GGATC 3 cut(s) 161, 393, 1192
AcoI YGGCCR 2 cut(s) 25, 58
AcsI RAATTY 2 cut(s) 49, 517
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 3 cut(s) 292, 320, 1066
AfiI CCNNNNNNNGG 5 cut(s) 34, 70, 358, 382, 910
AgsI TTSAA 8 cut(s) 181, 219, 484, 622, 766, 775, 926, 974
AluBI AGCT 4 cut(s) 280, 427, 977, 1073
AluI AGCT 4 cut(s) 280, 427, 977, 1073
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 1 cut(s) 530
AlwI GGATC 3 cut(s) 161, 393, 1192
AlwNI CAGNNNCTG 1 cut(s) 1073
Aor13HI TCCGGA 1 cut(s) 910
AoxI GGCC 6 cut(s) 25, 36, 58, 373, 438, 525
ApeKI GCWGC 3 cut(s) 277, 1073, 1076
ApoI RAATTY 2 cut(s) 49, 517
Asp700I GAANNNNTTC 1 cut(s) 812
AspS9I GGNCC 3 cut(s) 37, 374, 525
AsuHPI GGTGA 1 cut(s) 388
BanII GRGCYC 2 cut(s) 158, 429
BauI CACGAG 1 cut(s) 208
BbsI GAAGAC 2 cut(s) 763, 1130
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 3 cut(s) 289, 1060, 1063
BccI CCATC 4 cut(s) 341, 912, 1055, 1221
BceAI ACGGC 2 cut(s) 12, 388
BcoDI GTCTC 1 cut(s) 530
BfaI CTAG 1 cut(s) 966
BisI GCNGC 6 cut(s) 6, 278, 326, 360, 1074, 1077
BlsI GCNGC 6 cut(s) 7, 279, 327, 361, 1075, 1078
BmcAI AGTACT 1 cut(s) 292
BmgT120I GGNCC 3 cut(s) 37, 374, 525
BmiI GGNNCC 2 cut(s) 705, 1105
BmsI GCATC 3 cut(s) 558, 637, 659
BpiI GAAGAC 2 cut(s) 763, 1130
BpuEI CTTGAG 1 cut(s) 389
Bsa29I ATCGAT 1 cut(s) 1036
BsaBI GATNNNNATC 1 cut(s) 903
BsaJI CCNNGG 3 cut(s) 22, 252, 981
BsaWI WCCGGW 1 cut(s) 910
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 70, 358, 382, 910
Bse1I ACTGG 2 cut(s) 1023, 1186
Bse8I GATNNNNATC 1 cut(s) 903
BseAI TCCGGA 1 cut(s) 910
BseCI ATCGAT 1 cut(s) 1036
BseDI CCNNGG 3 cut(s) 22, 252, 981
BseGI GGATG 3 cut(s) 333, 919, 1147
BseJI GATNNNNATC 1 cut(s) 903
BseLI CCNNNNNNNGG 5 cut(s) 34, 70, 358, 382, 910
BseMII CTCAG 5 cut(s) 354, 361, 588, 708, 873
BseNI ACTGG 2 cut(s) 1023, 1186
BseRI GAGGAG 1 cut(s) 11
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 3 cut(s) 289, 1060, 1063
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 6 cut(s) 27, 38, 60, 375, 440, 527
BshVI ATCGAT 1 cut(s) 1036
BsiEI CGRYCG 1 cut(s) 28
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 2 cut(s) 57, 911
BslI CCNNNNNNNGG 5 cut(s) 34, 70, 358, 382, 910
BsmAI GTCTC 1 cut(s) 530
BsnI GGCC 6 cut(s) 27, 38, 60, 375, 440, 527
Bsp1286I GDGCHC 2 cut(s) 158, 429
Bsp13I TCCGGA 1 cut(s) 910
BspACI CCGC 5 cut(s) 5, 326, 359, 739, 858
BspANI GGCC 6 cut(s) 27, 38, 60, 375, 440, 527
BspCNI CTCAG 5 cut(s) 355, 360, 587, 707, 874
BspDI ATCGAT 1 cut(s) 1036
BspEI TCCGGA 1 cut(s) 910
BspHI TCATGA 1 cut(s) 711
BspLI GGNNCC 2 cut(s) 705, 1105
BspPI GGATC 3 cut(s) 161, 393, 1192
BspQI GCTCTTC 1 cut(s) 1098
BsrI ACTGG 2 cut(s) 1023, 1186
BssECI CCNNGG 3 cut(s) 22, 252, 981
BssNAI GTATAC 1 cut(s) 1028
BssSI CACGAG 1 cut(s) 208
Bst1107I GTATAC 1 cut(s) 1028
Bst2BI CACGAG 1 cut(s) 208
Bst4CI ACNGT 5 cut(s) 65, 761, 922, 985, 1087
Bst6I CTCTTC 1 cut(s) 1098
BstC8I GCNNGC 2 cut(s) 241, 858
BstDEI CTNAG 6 cut(s) 347, 363, 574, 694, 882, 895
BstDSI CCRYGG 1 cut(s) 981
BstF5I GGATG 3 cut(s) 333, 919, 1147
BstMAI GTCTC 1 cut(s) 530
BstMCI CGRYCG 1 cut(s) 28
BstV1I GCAGC 3 cut(s) 289, 1060, 1063
BstV2I GAAGAC 2 cut(s) 763, 1130
BstXI CCANNNNNNTGG 1 cut(s) 340
BstZ17I GTATAC 1 cut(s) 1028
BstZI CGGCCG 1 cut(s) 25
Bsu15I ATCGAT 1 cut(s) 1036
BsuRI GGCC 6 cut(s) 27, 38, 60, 375, 440, 527
BsuTUI ATCGAT 1 cut(s) 1036
BtgI CCRYGG 1 cut(s) 981
BtsCI GGATG 3 cut(s) 333, 919, 1147
BtsIMutI CAGTG 1 cut(s) 1179
Cac8I GCNNGC 2 cut(s) 241, 858
CaiI CAGNNNCTG 1 cut(s) 1073
CciI TCATGA 1 cut(s) 711
Cfr13I GGNCC 3 cut(s) 37, 374, 525
ClaI ATCGAT 1 cut(s) 1036
Csp6I GTAC 3 cut(s) 291, 319, 1065
CviAII CATG 5 cut(s) 340, 451, 522, 712, 820
CviQI GTAC 3 cut(s) 291, 319, 1065
DdeI CTNAG 6 cut(s) 347, 363, 574, 694, 882, 895
DraI TTTAAA 1 cut(s) 468
EaeI YGGCCR 2 cut(s) 25, 58
EagI CGGCCG 1 cut(s) 25
Eam1104I CTCTTC 1 cut(s) 1098
EarI CTCTTC 1 cut(s) 1098
Ecl136II GAGCTC 1 cut(s) 427
EclXI CGGCCG 1 cut(s) 25
Eco24I GRGCYC 2 cut(s) 158, 429
Eco52I CGGCCG 1 cut(s) 25
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 315
EcoICRI GAGCTC 1 cut(s) 427
EcoO109I RGGNCCY 1 cut(s) 37
EcoRI GAATTC 1 cut(s) 517
EcoT22I ATGCAT 2 cut(s) 613, 652
EcoT38I GRGCYC 2 cut(s) 158, 429
FaeI CATG 5 cut(s) 343, 454, 525, 715, 823
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FatI CATG 5 cut(s) 339, 450, 521, 711, 819
FauI CCCGC 1 cut(s) 865
FblI GTMKAC 2 cut(s) 186, 1027
Fnu4HI GCNGC 6 cut(s) 6, 278, 326, 360, 1074, 1077
FokI GGATG 3 cut(s) 320, 926, 1134
FriOI GRGCYC 2 cut(s) 158, 429
Fsp4HI GCNGC 6 cut(s) 6, 278, 326, 360, 1074, 1077
FspBI CTAG 1 cut(s) 966
GluI GCNGC 6 cut(s) 6, 278, 326, 360, 1074, 1077
HaeIII GGCC 6 cut(s) 27, 38, 60, 375, 440, 527
HapII CCGG 2 cut(s) 57, 911
Hin1II CATG 5 cut(s) 343, 454, 525, 715, 823
HincII GTYRAC 2 cut(s) 187, 580
HindII GTYRAC 2 cut(s) 187, 580
HindIII AAGCTT 1 cut(s) 975
HinfI GANTC 3 cut(s) 365, 884, 1114
HpaII CCGG 2 cut(s) 57, 911
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 6 cut(s) 32, 187, 319, 417, 580, 1028
Hpy188I TCNGA 7 cut(s) 139, 166, 204, 496, 883, 990, 1218
Hpy188III TCNNGA 6 cut(s) 654, 712, 797, 803, 911, 1118
Hpy8I GTNNAC 6 cut(s) 32, 187, 319, 417, 580, 1028
Hpy99I CGWCG 4 cut(s) 107, 110, 188, 191
HpyAV CCTTC 7 cut(s) 409, 526, 576, 628, 689, 729, 1162
HpyCH4III ACNGT 5 cut(s) 65, 761, 922, 985, 1087
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 3 cut(s) 549, 611, 650
HpyF3I CTNAG 6 cut(s) 347, 363, 574, 694, 882, 895
HpySE526I ACGT 1 cut(s) 183
Hsp92II CATG 5 cut(s) 343, 454, 525, 715, 823
Kpn2I TCCGGA 1 cut(s) 910
LguI GCTCTTC 1 cut(s) 1098
LmnI GCTCC 1 cut(s) 709
Lsp1109I GCAGC 3 cut(s) 289, 1060, 1063
LweI GCATC 3 cut(s) 558, 637, 659
MaeI CTAG 1 cut(s) 966
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 2 cut(s) 146, 1081
MhlI GDGCHC 2 cut(s) 158, 429
MluCI AATT 2 cut(s) 49, 517
MlyI GAGTC 2 cut(s) 374, 893
Mph1103I ATGCAT 2 cut(s) 613, 652
MroI TCCGGA 1 cut(s) 910
MroXI GAANNNNTTC 1 cut(s) 812
MseI TTAA 5 cut(s) 467, 699, 941, 954, 1053
MslI CAYNNNNRTG 3 cut(s) 338, 449, 824
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 2 cut(s) 57, 911
NlaIII CATG 5 cut(s) 343, 454, 525, 715, 823
NlaIV GGNNCC 2 cut(s) 705, 1105
NmeAIII GCCGAG 1 cut(s) 553
NsiI ATGCAT 2 cut(s) 613, 652
PagI TCATGA 1 cut(s) 711
PciSI GCTCTTC 1 cut(s) 1098
PdmI GAANNNNTTC 1 cut(s) 812
PfeI GAWTC 1 cut(s) 1114
PkrI GCNGC 6 cut(s) 7, 279, 327, 361, 1075, 1078
PleI GAGTC 2 cut(s) 373, 892
PpsI GAGTC 2 cut(s) 373, 892
Psp124BI GAGCTC 1 cut(s) 429
PspN4I GGNNCC 2 cut(s) 705, 1105
PspPI GGNCC 3 cut(s) 37, 374, 525
PstNI CAGNNNCTG 1 cut(s) 1073
PvuII CAGCTG 1 cut(s) 280
RsaI GTAC 3 cut(s) 292, 320, 1066
RsaNI GTAC 3 cut(s) 291, 319, 1065
RseI CAYNNNNRTG 3 cut(s) 338, 449, 824
SacI GAGCTC 1 cut(s) 429
SalI GTCGAC 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 1098
SaqAI TTAA 5 cut(s) 467, 699, 941, 954, 1053
SatI GCNGC 6 cut(s) 6, 278, 326, 360, 1074, 1077
Sau96I GGNCC 3 cut(s) 37, 374, 525
ScaI AGTACT 1 cut(s) 292
SchI GAGTC 2 cut(s) 374, 893
SduI GDGCHC 2 cut(s) 158, 429
SfaNI GCATC 3 cut(s) 558, 637, 659
SgrDI CGTCGACG 1 cut(s) 185
SmiMI CAYNNNNRTG 3 cut(s) 338, 449, 824
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 2 cut(s) 49, 517
SsiI CCGC 5 cut(s) 5, 326, 359, 739, 858
SspMI CTAG 1 cut(s) 966
SstI GAGCTC 1 cut(s) 429
TaaI ACNGT 5 cut(s) 65, 761, 922, 985, 1087
TaiI ACGT 1 cut(s) 186
TaqI TCGA 4 cut(s) 186, 477, 1036, 1221
TaqII GACCGA 1 cut(s) 266
TasI AATT 2 cut(s) 49, 517
TatI WGTACW 2 cut(s) 290, 318
TauI GCSGC 3 cut(s) 8, 328, 362
TfiI GAWTC 1 cut(s) 1114
Tru1I TTAA 5 cut(s) 467, 699, 941, 954, 1053
Tru9I TTAA 5 cut(s) 467, 699, 941, 954, 1053
TscAI CASTG 1 cut(s) 1186
TseI GCWGC 3 cut(s) 277, 1073, 1076
TspDTI ATGAA 9 cut(s) 236, 287, 510, 513, 700, 855, 930, 1033, 1186
TspGWI ACGGA 1 cut(s) 32
TspRI CASTG 1 cut(s) 1186
XapI RAATTY 2 cut(s) 49, 517
XcmI CCANNNNNNNNNTGG 1 cut(s) 683
XmiI GTMKAC 2 cut(s) 186, 1027
XmnI GAANNNNTTC 1 cut(s) 812
XspI CTAG 1 cut(s) 966
ZrmI AGTACT 1 cut(s) 292
Zsp2I ATGCAT 2 cut(s) 613, 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.