MD12G1126800.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000029
Physical Location & Seq
Reverse (-)
20184788 .. 20190856
6069 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1126800.v1.1.491

Sequence Viewer

Length: 2445 bp
ATGGCAGAACTTGTTACTTTTGCTGCCGAGGGGATACTGACTAGGGTGGCTTCACTAGCCGAGCAAGAATTCAGTCTCCTCTGGGGATTCAAAGGAGAAGTCGCACGGCTGCGGCAATCATTGTTCAAGATTCAAGCTATGCTGCGAGATGCGGCCCAGCATTCGCAGAATCGGGGCGAAGCGGTGCAAATTTGGGTGAAGAATCTTGAAGAAGTAGCTCAAGCTGCTGATCAAGTGCTGGACGAATACGAATACGAAGTTCTCCGGCGTCAGGTGGAACTTGAAAACCGAATGGAGAAAAAGGTGCTCAACTTCTTTTCACACCACAATCCCATTGCGTTTCGACACAATATGGGACGCAAAATCAAGAAAATCAATGCATTGTTGGCCAATCTGAGGGAGGAGGCAACTGGTTTGGGGCTAGTTGACACGAAAACAATGGATGCAACGTCTCATGATGGTATAAGAGTACCTGACAACAGGGTAACCGGCTCTGTCTTTGATCCAGATGAGAAGTACATTGTTGGAAGGGAGGAGGCTGTGTCGGAGATAGCTACAACCCTGATAAATTCCCGCAACAATAAGGAGAATTCTATTTCGGTTATGGCCATTGTGGGAATGGGAGGCCTGGGCAAGACAACTTTGGCTAAATCAGTTTATCATCATCCCGAGATAGGACGGTTCGATACAAAAATATGGATATGTGTATCTACTCCTTTCAATGTCACGGCGATTTTAAGCGGGATCTTAGAAAAGATTAAACCGGAAAAGGCTGGGATTAAGGGTAAAGCGACAATATGTGAAAATCTCCAAGAAGATTTGAAAGGAAAGCGATATCTTCTCGTTCTCGACGATGTCTGGAATGAGGATTCTCATAAATGGAATGATTTGATGAGTTGTTTGTCAAGTGTTGAAGATACCCAAGGAAGCAGCATCCTTGTCACTACCCGCAGTGCTAGTGTTGCATCAATTGTGGAAACACTTTCCAGGCGTGATTTGGAAAAATTATCGGATGACGAATGTTGGCGCATATTAAAGGATAGGGCATTTCGGAATGGGAGTGCTTCCGTGACCGAAGATCAAGAGCGAATGGGAAGGGAAATCGCCAAAAAATGTGCAGGTGTACCATTGGTGGCAAAGGTATTGGGAGATATGATGCGTCGTAAACCGAGTGGTGAATGGCAGTCAATTCAAGAAAGTACAATATGGAATTTGCAAGAAGGAGAAAGTACAATAAGGAATTTGCAAGGAGGAGAAAAAACAATCCTTTCAGTTTTGAAGTTGAGCTTTGTTGAATTGAGATCACCATCTTTGAAACAATGTTTTGCATATTGTTCAATGTTCGTCAAAGATGAGATTATTAAAAAGGAGGATTTAATCCAACTTTGGATGGCTCAAGGATTGCTCCAACCTTGTCCCAACAAAAGTATGGAGGATGTAGGGAATGAATATTTTAATATTCTATTGGAGAACTCCTTTTTTCAAGATGTTGAAATGGAAGAAAATACTGTTTACAGTTGCAAGATGCATGATCTTGTGCACGATCTTGCAGAACATGTGTCAAAATCAAAGAGCAAGGACTCCGATGAGAATCGACATATGGCAAAGCTCTCTACCTCGGCACTGCATGTAATTCCAAACGGAGATTTGAATGGTGATAAACTTGGTGGCATCTTCTCAAGATTTAAAGGTTTGCGCGTCTTAAAATTACGCGGTGCTGAAATTAGTGAGTTGCCAATTTCAATTAAAAAGTTGAAATGCTTGAGGTATCTTGATGTTTCCAATACATGGATAAAAAAGCTCCCCAAATCTATTGGCAAGCTTTATAATCTACAGACGTTGAAGATGCTATATGTCACAAGTTTTCCAGAGGAACTGCAAAATTTGATCAACTTGAGACATTTTGATTTTTCATTTGATGCGGAGAGATATCCAATTGGGATTGGGCGTTTGACTAATCTCCAAGCTTTACCTTCTTTCAGTGTGGGTAAGGAGAGAGGTCGTGGAATAGAGGAGTTGGCTGGCTTAAAGCAATTGAAAGGCCGATTATCCATATTTAATCTAGAGCATGTGAGAGATGGAGAGGAAGCGAAGAAAGCAAAACTAGCAGAGAAGACAAATCTGCGTGAATTAGTCTTTCGATGGGGATATGAGAAAGGGTCAAGCATTAATGCTGAGGATGTACTAGAAGGCCTTGGACCAGCACACCCTGAATGGGATGAAGACAGGTCTATGATCAACAATAATGATGAGGATGTACTAGAAGGTCTTCTACCAGCACACCCTGAACTGGAATATTTGAAGATTATATACTTTATGGGTGAGAAAAAACGTTGTGCTCTTTCCATGCCTCGAGGAGCTGTCTCTGACCTACTGCGATCAATTGAGAAGTGCTCCCAGTCATTTTCCATCTCTGAAAAAGTTAGAGATACATTCCATGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 ko04014 map03460 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

815

Amino Acids

92.01

Weight (kDa)

6.38

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 97 6.6e-20 Rx N-terminal domain
NB-ARC PF00931 178 - 352 6.8e-37 NB-ARC domain
WHD_DRP PF23559 447 - 517 5.6e-26 Disease resistance protein Winged helix domain
LRR_14 PF23598 559 - 711 1.4e-19 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 670 - 776 4.1e-15 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000029)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g13530 FvH4_4g18270 FvH4_6g12200 FvH4_6g12460 FvH4_6g12461 FvH4_6g13450 FvH4_6g15090 FvH4_6g15220 FvH4_6g15230 FvH4_6g15250 FvH4_6g15251 FvH4_6g15340 FvH4_6g47050 FvH4_6g47050 FvH4_6g47050 FvH4_6g47050 FvH4_6g47050 FvH4_6g47980 FvH4_6g47980 FvH4_6g47980 FvH4_6g48170 FvH4_6g48310 FvH4_6g48310 FvH4_6g48850 FvH4_6g48881 FvH4_7g11830
malus_domestica MD02G1059900.v1.1 MD02G1069600.v1.1 MD02G1069700.v1.1 MD02G1070100.v1.1 MD02G1070200.v1.1 MD02G1070300.v1.1 MD02G1070800.v1.1 MD02G1070900.v1.1 MD02G1071200.v1.1 MD02G1071400.v1.1 MD02G1071900.v1.1 MD02G1075200.v1.1 MD03G1048000.v1.1 MD03G1049100.v1.1 MD03G1049200.v1.1 MD03G1052500.v1.1 MD03G1054100.v1.1 MD03G1054300.v1.1 MD03G1054400.v1.1 MD04G1113400.v1.1 MD04G1211100.v1.1 MD04G1211200.v1.1 MD05G1174900.v1.1 MD05G1175000.v1.1 MD05G1175900.v1.1 MD05G1243400.v1.1 MD07G1031500.v1.1 MD07G1031600.v1.1 MD07G1032500.v1.1 MD11G1046800.v1.1 MD11G1046900.v1.1 MD11G1047000.v1.1 MD11G1050400.v1.1 MD11G1050600.v1.1 MD11G1050800.v1.1 MD11G1051100.v1.1 MD11G1051600.v1.1 MD11G1051700.v1.1 MD11G1054600.v1.1 MD11G1055600.v1.1 MD11G1055700.v1.1 MD11G1055800.v1.1 MD11G1056000.v1.1 MD12G1126600.v1.1 MD12G1126700.v1.1 MD12G1126800.v1.1 MD12G1127300.v1.1 MD12G1127600.v1.1 MD12G1127900.v1.1 MD12G1128100.v1.1 MD12G1128300.v1.1 MD15G1261500.v1.1
prunus_persica Prupe.1G010700_v2.0.a1 Prupe.1G010800_v2.0.a1 Prupe.1G011000_v2.0.a1 Prupe.1G159600_v2.0.a1 Prupe.1G163600_v2.0.a1 Prupe.1G163600_v2.0.a1 Prupe.1G163600_v2.0.a1 Prupe.1G163600_v2.0.a1 Prupe.1G163700_v2.0.a1 Prupe.1G163700_v2.0.a1 Prupe.1G163800_v2.0.a1 Prupe.1G164000_v2.0.a1 Prupe.1G164000_v2.0.a1 Prupe.1G164000_v2.0.a1 Prupe.1G164000_v2.0.a1 Prupe.1G235500_v2.0.a1 Prupe.2G067400_v2.0.a1 Prupe.2G067500_v2.0.a1 Prupe.2G111700_v2.0.a1 Prupe.2G111800_v2.0.a1 Prupe.3G012000_v2.0.a1 Prupe.6G243400_v2.0.a1 Prupe.6G243500_v2.0.a1 Prupe.6G243600_v2.0.a1 Prupe.6G243700_v2.0.a1 Prupe.6G243800_v2.0.a1 Prupe.6G243900_v2.0.a1
pyrus_communis pycom02g04860 pycom02g04880 pycom02g04890 pycom02g05510 pycom02g05520 pycom02g05600 pycom02g05930 pycom02g06000 pycom02g06010 pycom03g03800 pycom03g03860 pycom03g03880 pycom03g03890 pycom03g03910 pycom03g03920 pycom03g04200 pycom03g04290 pycom03g04300 pycom03g04310 pycom03g04320 pycom03g09260 pycom04g10150 pycom04g10160 pycom04g10170 pycom05g16180 pycom05g16190 pycom05g16240 pycom05g21980 pycom05g21990 pycom07g02280 pycom11g03800 pycom11g03810 pycom11g04080 pycom11g04140 pycom11g04150 pycom11g04160 pycom11g04570 pycom11g04580 pycom11g04590 pycom11g04610 pycom12g12470 pycom12g12490 pycom13g22050
rosa_chinensis RchiOBHm_Chr1g0317761 RchiOBHm_Chr1g0317821 RchiOBHm_Chr1g0317831 RchiOBHm_Chr1g0317871 RchiOBHm_Chr1g0350501 RchiOBHm_Chr2g0107631 RchiOBHm_Chr3g0452091 RchiOBHm_Chr3g0464001 RchiOBHm_Chr3g0464281 RchiOBHm_Chr3g0466331 RchiOBHm_Chr3g0467341 RchiOBHm_Chr3g0467381 RchiOBHm_Chr3g0467731 RchiOBHm_Chr3g0468291 RchiOBHm_Chr3g0468321 RchiOBHm_Chr3g0468341 RchiOBHm_Chr3g0468391 RchiOBHm_Chr3g0468411 RchiOBHm_Chr3g0468571 RchiOBHm_Chr3g0468591 RchiOBHm_Chr3g0490721 RchiOBHm_Chr3g0490751 RchiOBHm_Chr3g0490761 RchiOBHm_Chr3g0490771 RchiOBHm_Chr3g0490951 RchiOBHm_Chr3g0491001 RchiOBHm_Chr3g0491011 RchiOBHm_Chr4g0412461 RchiOBHm_Chr4g0414161 RchiOBHm_Chr4g0414181 RchiOBHm_Chr4g0414191 RchiOBHm_Chr4g0414281 RchiOBHm_Chr4g0414441 RchiOBHm_Chr4g0414521 RchiOBHm_Chr4g0414531 RchiOBHm_Chr4g0417011 RchiOBHm_Chr4g0417021 RchiOBHm_Chr4g0417041 RchiOBHm_Chr4g0417071 RchiOBHm_Chr4g0417101 RchiOBHm_Chr4g0417531 RchiOBHm_Chr4g0417641 RchiOBHm_Chr4g0417651 RchiOBHm_Chr4g0417701 RchiOBHm_Chr4g0417731 RchiOBHm_Chr4g0417741 RchiOBHm_Chr4g0417781 RchiOBHm_Chr4g0418751 RchiOBHm_Chr4g0418791 RchiOBHm_Chr4g0418861 RchiOBHm_Chr4g0418881 RchiOBHm_Chr4g0418901 RchiOBHm_Chr4g0422451 RchiOBHm_Chr4g0422461 RchiOBHm_Chr4g0422471 RchiOBHm_Chr4g0422541 RchiOBHm_Chr4g0422561 RchiOBHm_Chr4g0422591 RchiOBHm_Chr4g0422651 RchiOBHm_Chr4g0422661 RchiOBHm_Chr5g0003251 RchiOBHm_Chr5g0003261 RchiOBHm_Chr5g0055871 RchiOBHm_Chr7g0204701 RchiOBHm_Chr7g0204741
rosa_laevigata RLG00000007607 RLG00000007932 RLG00000017606 RLG00000024418 RLG00000025629 RLG00000030586
rosa_multiflora Rmu_co7959167.1_g000001 Rmu_co8021330.1_g000001 Rmu_co8132128.1_g000001 Rmu_co8224744.1_g000001 Rmu_co8281865.1_g000001 Rmu_co8298487.1_g000001 Rmu_co8319035.1_g000001 Rmu_co8380035.1_g000001 Rmu_co8385371.1_g000001 Rmu_co8398173.1_g000001 Rmu_co8423363.1_g000001 Rmu_co8438275.1_g000001 Rmu_co8474729.1_g000001 Rmu_sc0000057.1_g000008 Rmu_sc0000350.1_g000023 Rmu_sc0000480.1_g000005 Rmu_sc0000654.1_g000021 Rmu_sc0000654.1_g000029 Rmu_sc0000654.1_g000032 Rmu_sc0000654.1_g000041 Rmu_sc0000654.1_g000045 Rmu_sc0000772.1_g000002 Rmu_sc0000772.1_g000041 Rmu_sc0000808.1_g000040 Rmu_sc0000830.1_g000062 Rmu_sc0000830.1_g000065 Rmu_sc0000830.1_g000072 Rmu_sc0000830.1_g000073 Rmu_sc0000935.1_g000020 Rmu_sc0000935.1_g000024 Rmu_sc0000935.1_g000026 Rmu_sc0000935.1_g000027 Rmu_sc0000935.1_g000031 Rmu_sc0000935.1_g000033 Rmu_sc0000962.1_g000033 Rmu_sc0000962.1_g000036 Rmu_sc0000962.1_g000063 Rmu_sc0000962.1_g000064 Rmu_sc0000962.1_g000065 Rmu_sc0001029.1_g000007 Rmu_sc0001063.1_g000014 Rmu_sc0001149.1_g000010 Rmu_sc0001149.1_g000011 Rmu_sc0001358.1_g000009 Rmu_sc0001448.1_g000003 Rmu_sc0001448.1_g000004 Rmu_sc0001729.1_g000005 Rmu_sc0001729.1_g000007 Rmu_sc0001729.1_g000020 Rmu_sc0001729.1_g000023 Rmu_sc0001793.1_g000015 Rmu_sc0002494.1_g000018 Rmu_sc0002494.1_g000019 Rmu_sc0002604.1_g000022 Rmu_sc0002922.1_g000011 Rmu_sc0003008.1_g000009 Rmu_sc0003221.1_g000061 Rmu_sc0003221.1_g000062 Rmu_sc0003459.1_g000014 Rmu_sc0003480.1_g000015 Rmu_sc0003541.1_g000016 Rmu_sc0003541.1_g000020 Rmu_sc0004035.1_g000001 Rmu_sc0004035.1_g000005 Rmu_sc0004035.1_g000011 Rmu_sc0004142.1_g000004 Rmu_sc0004142.1_g000010 Rmu_sc0004353.1_g000008 Rmu_sc0004616.1_g000009 Rmu_sc0004616.1_g000010 Rmu_sc0005231.1_g000004 Rmu_sc0006380.1_g000025 Rmu_sc0006492.1_g000010 Rmu_sc0006895.1_g000001 Rmu_sc0006895.1_g000002 Rmu_sc0006895.1_g000005 Rmu_sc0007034.1_g000004 Rmu_sc0007104.1_g000006 Rmu_sc0008990.1_g000003 Rmu_sc0008990.1_g000004 Rmu_sc0009106.1_g000003 Rmu_sc0009516.1_g000002 Rmu_sc0009516.1_g000003 Rmu_sc0009516.1_g000004 Rmu_sc0009516.1_g000005 Rmu_sc0009645.1_g000001 Rmu_sc0010274.1_g000001 Rmu_sc0010274.1_g000002 Rmu_sc0010419.1_g000003 Rmu_sc0010917.1_g000017 Rmu_sc0011213.1_g000001 Rmu_sc0011228.1_g000007 Rmu_sc0011538.1_g000011 Rmu_sc0011538.1_g000014 Rmu_sc0011538.1_g000015 Rmu_sc0012211.1_g000006 Rmu_sc0012351.1_g000003 Rmu_sc0012351.1_g000005 Rmu_sc0012915.1_g000002 Rmu_sc0013088.1_g000001 Rmu_sc0013459.1_g000001 Rmu_sc0013765.1_g000004 Rmu_sc0013765.1_g000005 Rmu_sc0014003.1_g000012 Rmu_sc0014003.1_g000014 Rmu_sc0014698.1_g000001 Rmu_sc0015740.1_g000001 Rmu_sc0016429.1_g000003 Rmu_sc0018885.1_g000001 Rmu_sc0020345.1_g000001 Rmu_sc0027791.1_g000001 Rmu_sc0037325.1_g000001 Rmu_ssc0000010.1_g000024 Rmu_ssc0000010.1_g000025 Rmu_ssc0000083.1_g000021 Rmu_ssc0000340.1_g000002 Rmu_ssc0000386.1_g000002 Rmu_ssc0000386.1_g000024 Rmu_ssc0000386.1_g000025 Rmu_ssc0000386.1_g000034 Rmu_ssc0000386.1_g000037 Rmu_ssc0000477.1_g000001
rosa_roxburghii Rroxscaffold_161G00448010 Rroxscaffold_164G00436080 Rroxscaffold_1G00024500 Rroxscaffold_2G00124290 Rroxscaffold_3G00222940 Rroxscaffold_3G00227440 Rroxscaffold_4G00304810 Rroxscaffold_4G00330180 Rroxscaffold_4G00330200 Rroxscaffold_5G00356620 Rroxscaffold_5G00358170 Rroxscaffold_5G00360880 Rroxscaffold_5G00361220 Rroxscaffold_5G00361950 Rroxscaffold_5G00361960 Rroxscaffold_5G00361990 Rroxscaffold_5G00362370 Rroxscaffold_5G00364540 Rroxscaffold_5G00364550 Rroxscaffold_5G00364580 Rroxscaffold_5G00364600 Rroxscaffold_6G00393290 Rroxscaffold_6G00412680 Rroxscaffold_6G00412790 Rroxscaffold_6G00413470 Rroxscaffold_6G00413720 Rroxscaffold_6G00416070 Rroxscaffold_7G00205400
rosa_rugosa Rorug01G0017300 Rorug01G0017400 Rorug01G0018000 Rorug01G0018100 Rorug01G0211500 Rorug02G0638200 Rorug03G0070000 Rorug03G0071200 Rorug03G0081000 Rorug03G0086500 Rorug03G0089300 Rorug03G0095300 Rorug03G0096000 Rorug03G0096000 Rorug04G0055000 Rorug04G0078300 Rorug04G0104100.1 Rorug04G0113800 Rorug04G0126700 Rorug04G0128000 Rorug04G0145900 Rorug04G0148700 Rorug04G0149100 Rorug04G0149100 Rorug04G0149200 Rorug04G0149300 Rorug04G0154900 Rorug04G0180400 Rorug04G0180500 Rorug04G0180700 Rorug04G0180800 Rorug04G0401500 Rorug04G0402100 Rorug05G0288600 Rorug05G0334100 Rorug07G0176400
rosa_samantha Rh1DG043200 Rh1DG043500 Rh1DG043900 Rh1DG222800 Rh3CG039900 Rh3CG156200 Rh3CG328500 Rh3CG328800 Rh3DG165000 Rh4AG208400 Rh4BG205600 Rh4BG213900 Rh4BG243900 Rh4CG198800 Rh4DG168600 Rh4DG180100 Rh4DG180300 Rh4DG180600 Rh4DG181200 Rh4DG181600 Rh4DG181700 Rh4DG183700 Rh4DG184000 Rh4DG184400 Rh4DG184700 Rh4DG206100 Rh4DG206200 Rh4DG206300 Rh4DG215600 Rh4DG238700 Rh4DG238900 Rh5DG390300
rosa_wichuraiana Rw0G005050 Rw0G009260 Rw0G009280 Rw0G015600 Rw0G023570 Rw1G002140 Rw1G002170 Rw1G002210 Rw1G002240 Rw1G019500 Rw2G015490 Rw3G003020 Rw3G010900 Rw3G012030 Rw3G012790 Rw3G013050 Rw3G013540 Rw3G013640 Rw3G013650 Rw3G026050 Rw3G026070 Rw4G015740 Rw4G015790 Rw4G015910 Rw4G015930 Rw4G015950 Rw4G017900 Rw4G017930 Rw4G018440 Rw4G018450 Rw4G018530 Rw4G020720 Rw4G020760 Rw5G019250 Rw5G034370 Rw7G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1827
AarI CACCTGC 1 cut(s) 1109
AbsI CCTCGAGG 1 cut(s) 2352
Acc36I ACCTGC 1 cut(s) 1109
AccB7I CCANNNNNTGG 1 cut(s) 1788
AccII CGCG 2 cut(s) 1698, 1713
AciI CCGC 8 cut(s) 112, 152, 182, 574, 741, 949, 1713, 1922
AclI AACGTT 1 cut(s) 2332
AclWI GGATC 2 cut(s) 497, 752
AcoI YGGCCR 2 cut(s) 387, 606
AcsI RAATTY 7 cut(s) 68, 189, 568, 589, 1210, 1240, 1882
AcyI GRCGYC 1 cut(s) 268
AfaI GTAC 7 cut(s) 471, 518, 1125, 1201, 1231, 2184, 2259
AfiI CCNNNNNNNGG 6 cut(s) 271, 396, 674, 1788, 2215, 2290
AflIII ACRYGT 1 cut(s) 1555
AjnI CCWGG 2 cut(s) 627, 986
Alw21I GWGCWC 4 cut(s) 309, 1542, 2341, 2396
Alw26I GTCTC 4 cut(s) 80, 456, 1891, 2368
Alw44I GTGCAC 1 cut(s) 1538
AlwI GGATC 2 cut(s) 497, 752
Ama87I CYCGRG 2 cut(s) 668, 2352
AoxI GGCC 6 cut(s) 153, 387, 606, 625, 2041, 2191
ApaLI GTGCAC 1 cut(s) 1538
ApeKI GCWGC 5 cut(s) 23, 109, 142, 224, 930
ApoI RAATTY 7 cut(s) 68, 189, 568, 589, 1210, 1240, 1882
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AseI ATTAAT 1 cut(s) 2169
Asp700I GAANNNNTTC 1 cut(s) 2268
AspLEI GCGC 2 cut(s) 1029, 1698
AspS9I GGNCC 2 cut(s) 154, 2198
AsuHPI GGTGA 5 cut(s) 208, 1187, 1296, 1667, 2333
AvaI CYCGRG 2 cut(s) 668, 2352
AvaII GGWCC 1 cut(s) 2198
BaeGI GKGCMC 1 cut(s) 1542
BalI TGGCCA 2 cut(s) 389, 608
BarI GAAGNNNNNNTAC 4 cut(s) 2256, 2288, 2294, 2326
BbsI GAAGAC 3 cut(s) 2120, 2229, 2261
Bbv12I GWGCWC 4 cut(s) 309, 1542, 2341, 2396
BbvCI CCTCAGC 1 cut(s) 2175
BbvI GCAGC 5 cut(s) 10, 96, 129, 211, 942
BccI CCATC 6 cut(s) 452, 1315, 1384, 2072, 2136, 2417
BceAI ACGGC 2 cut(s) 122, 744
BciT130I CCWGG 2 cut(s) 629, 988
BciVI GTATCC 1 cut(s) 27
BclI TGATCA 3 cut(s) 229, 1887, 2235
BcoDI GTCTC 4 cut(s) 80, 456, 1891, 2368
BfaI CTAG 8 cut(s) 42, 56, 422, 957, 2063, 2105, 2186, 2261
BfmI CTRYAG 1 cut(s) 1832
BfuAI ACCTGC 1 cut(s) 1109
BfuI GTATCC 1 cut(s) 27
BisI GCNGC 7 cut(s) 24, 110, 113, 143, 153, 225, 931
BlsI GCNGC 7 cut(s) 25, 111, 114, 144, 154, 226, 932
Bme1390I CCNGG 2 cut(s) 629, 988
Bme18I GGWCC 1 cut(s) 2198
BmeT110I CYCGRG 2 cut(s) 668, 2352
BmgT120I GGNCC 2 cut(s) 154, 2198
BmrFI CCNGG 2 cut(s) 629, 988
BmrI ACTGGG 1 cut(s) 2392
BmsI GCATC 9 cut(s) 139, 433, 942, 974, 1146, 1515, 1680, 1836, 1909
BmuI ACTGGG 1 cut(s) 2392
BpiI GAAGAC 3 cut(s) 2120, 2229, 2261
BplI GAGNNNNNCTC 2 cut(s) 2378, 2410
Bpu10I CCTNAGC 1 cut(s) 2175
BpuEI CTTGAG 5 cut(s) 204, 1380, 1663, 1783, 1915
BsaBI GATNNNNATC 2 cut(s) 1306, 1590
BsaHI GRCGYC 1 cut(s) 268
BsaJI CCNNGG 6 cut(s) 27, 628, 922, 1617, 2194, 2437
BsaWI WCCGGW 1 cut(s) 763
BsaXI ACNNNNNCTCC 2 cut(s) 527, 557
Bsc4I CCNNNNNNNGG 6 cut(s) 271, 396, 674, 1788, 2215, 2290
Bse118I RCCGGY 1 cut(s) 488
Bse1I ACTGG 3 cut(s) 415, 2295, 2398
Bse3DI GCAATG 1 cut(s) 333
Bse8I GATNNNNATC 2 cut(s) 1306, 1590
BseBI CCWGG 2 cut(s) 629, 988
BseDI CCNNGG 6 cut(s) 27, 628, 922, 1617, 2194, 2437
BseGI GGATG 9 cut(s) 448, 664, 933, 1018, 1395, 1441, 2185, 2224, 2260
BseJI GATNNNNATC 2 cut(s) 1306, 1590
BseLI CCNNNNNNNGG 6 cut(s) 271, 396, 674, 1788, 2215, 2290
BseMI GCAATG 1 cut(s) 333
BseMII CTCAG 2 cut(s) 386, 2166
BseNI ACTGG 3 cut(s) 415, 2295, 2398
BseRI GAGGAG 6 cut(s) 68, 416, 548, 1266, 2027, 2370
BseSI GKGCMC 1 cut(s) 1542
BseXI GCAGC 5 cut(s) 10, 96, 129, 211, 942
BseYI CCCAGC 2 cut(s) 156, 773
BsgI GTGCAG 1 cut(s) 1137
Bsh1236I CGCG 2 cut(s) 1698, 1713
BshFI GGCC 6 cut(s) 155, 389, 608, 627, 2043, 2193
BsiHKAI GWGCWC 4 cut(s) 309, 1542, 2341, 2396
BsiHKCI CYCGRG 2 cut(s) 668, 2352
BsiSI CCGG 3 cut(s) 265, 489, 764
BslFI GGGAC 2 cut(s) 369, 1401
BslI CCNNNNNNNGG 6 cut(s) 271, 396, 674, 1788, 2215, 2290
BsmAI GTCTC 4 cut(s) 80, 456, 1891, 2368
BsmBI CGTCTC 1 cut(s) 456
BsmFI GGGAC 2 cut(s) 369, 1401
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 6 cut(s) 155, 389, 608, 627, 2043, 2193
BsoBI CYCGRG 2 cut(s) 668, 2352
Bsp1286I GDGCHC 4 cut(s) 309, 1542, 2341, 2396
Bsp19I CCATGG 1 cut(s) 2437
BspACI CCGC 8 cut(s) 112, 152, 182, 574, 741, 949, 1713, 1922
BspANI GGCC 6 cut(s) 155, 389, 608, 627, 2043, 2193
BspCNI CTCAG 2 cut(s) 387, 2167
BspFNI CGCG 2 cut(s) 1698, 1713
BspHI TCATGA 1 cut(s) 454
BspMI ACCTGC 1 cut(s) 1109
BspPI GGATC 2 cut(s) 497, 752
BsrDI GCAATG 1 cut(s) 333
BsrFI RCCGGY 1 cut(s) 488
BsrI ACTGG 3 cut(s) 415, 2295, 2398
BssAI RCCGGY 1 cut(s) 488
BssECI CCNNGG 6 cut(s) 27, 628, 922, 1617, 2194, 2437
BssNI GRCGYC 1 cut(s) 268
BssT1I CCWWGG 3 cut(s) 922, 2194, 2437
Bst2UI CCWGG 2 cut(s) 629, 988
Bst4CI ACNGT 3 cut(s) 681, 1510, 1517
BstACI GRCGYC 1 cut(s) 268
BstC8I GCNNGC 2 cut(s) 1820, 2023
BstDEI CTNAG 3 cut(s) 395, 748, 2175
BstDSI CCRYGG 1 cut(s) 2437
BstEII GGTNACC 1 cut(s) 484
BstF5I GGATG 9 cut(s) 448, 664, 933, 1018, 1395, 1441, 2185, 2224, 2260
BstFNI CGCG 2 cut(s) 1698, 1713
BstHHI GCGC 2 cut(s) 1029, 1698
BstMAI GTCTC 4 cut(s) 80, 456, 1891, 2368
BstMWI GCNNNNNNNGC 6 cut(s) 56, 224, 386, 962, 2096, 2105
BstNI CCWGG 2 cut(s) 629, 988
BstNSI RCATGY 3 cut(s) 1559, 1631, 2072
BstPI GGTNACC 1 cut(s) 484
BstSCI CCNGG 2 cut(s) 627, 986
BstSFI CTRYAG 1 cut(s) 1832
BstSLI GKGCMC 1 cut(s) 1542
BstUI CGCG 2 cut(s) 1698, 1713
BstV1I GCAGC 5 cut(s) 10, 96, 129, 211, 942
BstV2I GAAGAC 3 cut(s) 2120, 2229, 2261
BstX2I RGATCY 1 cut(s) 744
BstYI RGATCY 1 cut(s) 744
BsuI GTATCC 1 cut(s) 27
BsuRI GGCC 6 cut(s) 155, 389, 608, 627, 2043, 2193
BtgI CCRYGG 1 cut(s) 2437
BtsCI GGATG 9 cut(s) 448, 664, 933, 1018, 1395, 1441, 2185, 2224, 2260
BtsI GCAGTG 2 cut(s) 958, 1622
BtsIMutI CAGTG 3 cut(s) 958, 1622, 1987
BveI ACCTGC 1 cut(s) 1109
Cac8I GCNNGC 2 cut(s) 1820, 2023
CciI TCATGA 1 cut(s) 454
CfoI GCGC 2 cut(s) 1029, 1698
Cfr10I RCCGGY 1 cut(s) 488
Cfr13I GGNCC 2 cut(s) 154, 2198
CseI GACGC 4 cut(s) 257, 366, 1148, 1687
Csp6I GTAC 7 cut(s) 470, 517, 1124, 1200, 1230, 2183, 2258
CviAII CATG 8 cut(s) 455, 1529, 1556, 1628, 1788, 2069, 2347, 2438
CviQI GTAC 7 cut(s) 470, 517, 1124, 1200, 1230, 2183, 2258
DdeI CTNAG 3 cut(s) 395, 748, 2175
DraI TTTAAA 1 cut(s) 1687
EaeI YGGCCR 2 cut(s) 387, 606
Eco130I CCWWGG 3 cut(s) 922, 2194, 2437
Eco147I AGGCCT 2 cut(s) 627, 2193
Eco32I GATATC 2 cut(s) 836, 1931
Eco47I GGWCC 1 cut(s) 2198
Eco88I CYCGRG 2 cut(s) 668, 2352
Eco91I GGTNACC 1 cut(s) 484
EcoO65I GGTNACC 1 cut(s) 484
EcoRI GAATTC 2 cut(s) 68, 589
EcoRII CCWGG 2 cut(s) 627, 986
EcoRV GATATC 2 cut(s) 836, 1931
EcoT14I CCWWGG 3 cut(s) 922, 2194, 2437
EcoT22I ATGCAT 2 cut(s) 382, 1530
ErhI CCWWGG 3 cut(s) 922, 2194, 2437
Esp3I CGTCTC 1 cut(s) 456
FaeI CATG 8 cut(s) 458, 1532, 1559, 1631, 1791, 2072, 2350, 2441
FalI AAGNNNNNCTT 2 cut(s) 1271, 1303
FaqI GGGAC 2 cut(s) 369, 1401
FatI CATG 8 cut(s) 454, 1528, 1555, 1627, 1787, 2068, 2346, 2437
FauI CCCGC 3 cut(s) 581, 734, 956
FauNDI CATATG 1 cut(s) 1599
FbaI TGATCA 3 cut(s) 229, 1887, 2235
Fnu4HI GCNGC 7 cut(s) 24, 110, 113, 143, 153, 225, 931
FokI GGATG 9 cut(s) 455, 651, 920, 1025, 1402, 1448, 2192, 2231, 2267
Fsp4HI GCNGC 7 cut(s) 24, 110, 113, 143, 153, 225, 931
FspBI CTAG 8 cut(s) 42, 56, 422, 957, 2063, 2105, 2186, 2261
GlaI GCGC 2 cut(s) 1028, 1697
GluI GCNGC 7 cut(s) 24, 110, 113, 143, 153, 225, 931
GsaI CCCAGC 2 cut(s) 160, 777
HaeIII GGCC 6 cut(s) 155, 389, 608, 627, 2043, 2193
HapII CCGG 3 cut(s) 265, 489, 764
HgaI GACGC 4 cut(s) 257, 366, 1148, 1687
HhaI GCGC 2 cut(s) 1029, 1698
Hin1I GRCGYC 1 cut(s) 268
Hin1II CATG 8 cut(s) 458, 1532, 1559, 1631, 1791, 2072, 2350, 2441
Hin6I GCGC 2 cut(s) 1027, 1696
HinP1I GCGC 2 cut(s) 1027, 1696
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 2 cut(s) 1820, 1965
HinfI GANTC 7 cut(s) 87, 130, 169, 202, 869, 1580, 1591
HpaII CCGG 3 cut(s) 265, 489, 764
HphI GGTGA 5 cut(s) 208, 1187, 1296, 1667, 2333
Hpy166II GTNNAC 5 cut(s) 427, 1124, 1166, 1513, 1540
Hpy188I TCNGA 7 cut(s) 396, 547, 1012, 1053, 1585, 2368, 2416
Hpy8I GTNNAC 5 cut(s) 427, 1124, 1166, 1513, 1540
Hpy99I CGWCG 2 cut(s) 854, 1164
HpyAV CCTTC 6 cut(s) 522, 1089, 1214, 1983, 2183, 2258
HpyCH4III ACNGT 3 cut(s) 681, 1510, 1517
HpyCH4IV ACGT 3 cut(s) 449, 1838, 2332
HpyF10VI GCNNNNNNNGC 6 cut(s) 56, 224, 386, 962, 2096, 2105
HpyF3I CTNAG 3 cut(s) 395, 748, 2175
HpySE526I ACGT 3 cut(s) 449, 1838, 2332
Hsp92I GRCGYC 1 cut(s) 268
Hsp92II CATG 8 cut(s) 458, 1532, 1559, 1631, 1791, 2072, 2350, 2441
HspAI GCGC 2 cut(s) 1027, 1696
Ksp22I TGATCA 3 cut(s) 229, 1887, 2235
LmnI GCTCC 4 cut(s) 1410, 1806, 2357, 2399
Lsp1109I GCAGC 5 cut(s) 10, 96, 129, 211, 942
LweI GCATC 9 cut(s) 139, 433, 942, 974, 1146, 1515, 1680, 1836, 1909
MaeI CTAG 8 cut(s) 42, 56, 422, 957, 2063, 2105, 2186, 2261
MaeII ACGT 3 cut(s) 449, 1838, 2332
MaeIII GTNAC 6 cut(s) 13, 484, 724, 940, 1069, 1855
MfeI CAATTG 4 cut(s) 969, 1935, 2033, 2382
MflI RGATCY 1 cut(s) 744
MhlI GDGCHC 4 cut(s) 309, 1542, 2341, 2396
MlsI TGGCCA 2 cut(s) 389, 608
MluNI TGGCCA 2 cut(s) 389, 608
MlyI GAGTC 1 cut(s) 1574
MmeI TCCRAC 4 cut(s) 505, 525, 1405, 1432
Mox20I TGGCCA 2 cut(s) 389, 608
Mph1103I ATGCAT 2 cut(s) 382, 1530
MroXI GAANNNNTTC 1 cut(s) 2268
MscI TGGCCA 2 cut(s) 389, 608
Msp20I TGGCCA 2 cut(s) 389, 608
MspI CCGG 3 cut(s) 265, 489, 764
MspR9I CCNGG 2 cut(s) 629, 988
MunI CAATTG 4 cut(s) 969, 1935, 2033, 2382
Mva1269I GAATGC 1 cut(s) 160
MvaI CCWGG 2 cut(s) 629, 988
MvnI CGCG 2 cut(s) 1698, 1713
MwoI GCNNNNNNNGC 6 cut(s) 56, 224, 386, 962, 2096, 2105
NcoI CCATGG 1 cut(s) 2437
NdeI CATATG 1 cut(s) 1599
NlaIII CATG 8 cut(s) 458, 1532, 1559, 1631, 1791, 2072, 2350, 2441
NmeAIII GCCGAG 3 cut(s) 52, 85, 1598
NmuCI GTSAC 4 cut(s) 724, 940, 1069, 1855
NsiI ATGCAT 2 cut(s) 382, 1530
NspI RCATGY 3 cut(s) 1559, 1631, 2072
PaeR7I CTCGAG 1 cut(s) 2352
PagI TCATGA 1 cut(s) 454
PaqCI CACCTGC 1 cut(s) 1109
PceI AGGCCT 2 cut(s) 627, 2193
PciI ACATGT 1 cut(s) 1555
PcsI WCGNNNNNNNCGW 1 cut(s) 849
PctI GAATGC 1 cut(s) 160
PdmI GAANNNNTTC 1 cut(s) 2268
PfeI GAWTC 6 cut(s) 87, 130, 169, 202, 869, 1591
PflFI GACNNNGTC 1 cut(s) 854
PflMI CCANNNNNTGG 1 cut(s) 1788
PkrI GCNGC 7 cut(s) 25, 111, 114, 144, 154, 226, 932
PleI GAGTC 1 cut(s) 1574
PpsI GAGTC 1 cut(s) 1574
PscI ACATGT 1 cut(s) 1555
PshBI ATTAAT 1 cut(s) 2169
PsiI TTATAA 1 cut(s) 1827
Psp1406I AACGTT 1 cut(s) 2332
Psp6I CCWGG 2 cut(s) 627, 986
PspEI GGTNACC 1 cut(s) 484
PspFI CCCAGC 2 cut(s) 156, 773
PspGI CCWGG 2 cut(s) 627, 986
PspPI GGNCC 2 cut(s) 154, 2198
PspXI VCTCGAGB 1 cut(s) 2352
PsuI RGATCY 1 cut(s) 744
PsyI GACNNNGTC 1 cut(s) 854
RsaI GTAC 7 cut(s) 471, 518, 1125, 1201, 1231, 2184, 2259
RsaNI GTAC 7 cut(s) 470, 517, 1124, 1200, 1230, 2183, 2258
SatI GCNGC 7 cut(s) 24, 110, 113, 143, 153, 225, 931
Sau96I GGNCC 2 cut(s) 154, 2198
SchI GAGTC 1 cut(s) 1574
ScrFI CCNGG 2 cut(s) 629, 988
SduI GDGCHC 4 cut(s) 309, 1542, 2341, 2396
SfaNI GCATC 9 cut(s) 139, 433, 942, 974, 1146, 1515, 1680, 1836, 1909
SfcI CTRYAG 1 cut(s) 1832
Sfr274I CTCGAG 1 cut(s) 2352
SinI GGWCC 1 cut(s) 2198
SlaI CTCGAG 1 cut(s) 2352
SmlI CTYRAG 6 cut(s) 219, 1395, 1678, 1762, 1894, 2352
SmoI CTYRAG 6 cut(s) 219, 1395, 1678, 1762, 1894, 2352
SseBI AGGCCT 2 cut(s) 627, 2193
SsiI CCGC 8 cut(s) 112, 152, 182, 574, 741, 949, 1713, 1922
SspI AATATT 3 cut(s) 1451, 1459, 2297
SspMI CTAG 8 cut(s) 42, 56, 422, 957, 2063, 2105, 2186, 2261
StuI AGGCCT 2 cut(s) 627, 2193
StyD4I CCNGG 2 cut(s) 627, 986
StyI CCWWGG 3 cut(s) 922, 2194, 2437
TaaI ACNGT 3 cut(s) 681, 1510, 1517
TaiI ACGT 3 cut(s) 452, 1841, 2335
TaqI TCGA 6 cut(s) 343, 684, 849, 1594, 2140, 2353
TaqII GACCGA 1 cut(s) 1088
TatI WGTACW 5 cut(s) 516, 1199, 1229, 2182, 2257
TauI GCSGC 2 cut(s) 115, 155
TfiI GAWTC 6 cut(s) 87, 130, 169, 202, 869, 1591
TscAI CASTG 3 cut(s) 958, 1629, 1987
TseFI GTSAC 4 cut(s) 724, 940, 1069, 1855
TseI GCWGC 5 cut(s) 23, 109, 142, 224, 930
Tsp45I GTSAC 4 cut(s) 724, 940, 1069, 1855
TspDTI ATGAA 3 cut(s) 1461, 1902, 2235
TspGWI ACGGA 2 cut(s) 1057, 1656
TspRI CASTG 3 cut(s) 958, 1629, 1987
Tth111I GACNNNGTC 1 cut(s) 854
Van91I CCANNNNNTGG 1 cut(s) 1788
VneI GTGCAC 1 cut(s) 1538
VpaK11BI GGWCC 1 cut(s) 2198
VspI ATTAAT 1 cut(s) 2169
XapI RAATTY 7 cut(s) 68, 189, 568, 589, 1210, 1240, 1882
XbaI TCTAGA 1 cut(s) 2062
XceI RCATGY 3 cut(s) 1559, 1631, 2072
XcmI CCANNNNNNNNNTGG 3 cut(s) 616, 994, 1426
XhoI CTCGAG 1 cut(s) 2352
XmnI GAANNNNTTC 1 cut(s) 2268
XspI CTAG 8 cut(s) 42, 56, 422, 957, 2063, 2105, 2186, 2261
Zsp2I ATGCAT 2 cut(s) 382, 1530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.