ADP binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 741 |
| AciI | CCGC | 2 cut(s) | 611, 665 |
| AclWI | GGATC | 4 cut(s) | 429, 936, 949, 1027 |
| AcoI | YGGCCR | 2 cut(s) | 618, 657 |
| AcsI | RAATTY | 5 cut(s) | 11, 74, 275, 393, 691 |
| AcuI | CTGAAG | 1 cut(s) | 270 |
| AcyI | GRCGYC | 1 cut(s) | 738 |
| AfaI | GTAC | 2 cut(s) | 284, 864 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 382, 543 |
| AgsI | TTSAA | 7 cut(s) | 97, 140, 151, 280, 380, 701, 841 |
| AhlI | ACTAGT | 1 cut(s) | 451 |
| AjnI | CCWGG | 1 cut(s) | 458 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 522, 554 |
| AluBI | AGCT | 5 cut(s) | 101, 115, 206, 248, 925 |
| AluI | AGCT | 5 cut(s) | 101, 115, 206, 248, 925 |
| AlwI | GGATC | 4 cut(s) | 429, 936, 949, 1027 |
| Ama87I | CYCGRG | 1 cut(s) | 425 |
| AoxI | GGCC | 4 cut(s) | 296, 618, 637, 657 |
| ApoI | RAATTY | 5 cut(s) | 11, 74, 275, 393, 691 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 939, 971 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 731 |
| AspLEI | GCGC | 1 cut(s) | 388 |
| AspS9I | GGNCC | 1 cut(s) | 1046 |
| AsuHPI | GGTGA | 2 cut(s) | 238, 563 |
| AsuII | TTCGAA | 1 cut(s) | 794 |
| AvaI | CYCGRG | 1 cut(s) | 425 |
| AvaII | GGWCC | 1 cut(s) | 1046 |
| BalI | TGGCCA | 2 cut(s) | 620, 659 |
| BamHI | GGATCC | 1 cut(s) | 941 |
| BbvCI | CCTCAGC | 2 cut(s) | 66, 258 |
| BciT130I | CCWGG | 1 cut(s) | 460 |
| BciVI | GTATCC | 1 cut(s) | 421 |
| BcuI | ACTAGT | 1 cut(s) | 451 |
| BfaI | CTAG | 1 cut(s) | 452 |
| BfuI | GTATCC | 1 cut(s) | 421 |
| Bme1390I | CCNGG | 1 cut(s) | 460 |
| Bme18I | GGWCC | 1 cut(s) | 1046 |
| BmeT110I | CYCGRG | 1 cut(s) | 425 |
| BmgT120I | GGNCC | 1 cut(s) | 1046 |
| BmiI | GGNNCC | 1 cut(s) | 943 |
| BmrFI | CCNGG | 1 cut(s) | 460 |
| BmsI | GCATC | 5 cut(s) | 14, 244, 446, 460, 989 |
| Bpu10I | CCTNAGC | 2 cut(s) | 66, 258 |
| Bpu14I | TTCGAA | 1 cut(s) | 794 |
| Bsa29I | ATCGAT | 1 cut(s) | 1022 |
| BsaHI | GRCGYC | 1 cut(s) | 738 |
| BsaJI | CCNNGG | 3 cut(s) | 414, 640, 660 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 382, 543 |
| Bse1I | ACTGG | 2 cut(s) | 48, 785 |
| BseBI | CCWGG | 1 cut(s) | 460 |
| BseCI | ATCGAT | 1 cut(s) | 1022 |
| BseDI | CCNNGG | 3 cut(s) | 414, 640, 660 |
| BseGI | GGATG | 3 cut(s) | 268, 277, 1041 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 382, 543 |
| BseMII | CTCAG | 3 cut(s) | 57, 249, 1002 |
| BseNI | ACTGG | 2 cut(s) | 48, 785 |
| BseRI | GAGGAG | 1 cut(s) | 432 |
| BshFI | GGCC | 4 cut(s) | 298, 620, 639, 659 |
| BshVI | ATCGAT | 1 cut(s) | 1022 |
| BsiHKCI | CYCGRG | 1 cut(s) | 425 |
| BslFI | GGGAC | 3 cut(s) | 509, 773, 808 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 382, 543 |
| BsmFI | GGGAC | 3 cut(s) | 509, 773, 808 |
| BsnI | GGCC | 4 cut(s) | 298, 620, 639, 659 |
| BsoBI | CYCGRG | 1 cut(s) | 425 |
| Bsp119I | TTCGAA | 1 cut(s) | 794 |
| Bsp143I | GATC | 7 cut(s) | 292, 402, 421, 883, 941, 1019, 1023 |
| BspACI | CCGC | 2 cut(s) | 611, 665 |
| BspANI | GGCC | 4 cut(s) | 298, 620, 639, 659 |
| BspCNI | CTCAG | 3 cut(s) | 58, 250, 1001 |
| BspDI | ATCGAT | 1 cut(s) | 1022 |
| BspHI | TCATGA | 1 cut(s) | 1052 |
| BspLI | GGNNCC | 1 cut(s) | 943 |
| BspPI | GGATC | 4 cut(s) | 429, 936, 949, 1027 |
| BspT104I | TTCGAA | 1 cut(s) | 794 |
| BsrI | ACTGG | 2 cut(s) | 48, 785 |
| BssECI | CCNNGG | 3 cut(s) | 414, 640, 660 |
| BssMI | GATC | 7 cut(s) | 292, 402, 421, 883, 941, 1019, 1023 |
| BssNI | GRCGYC | 1 cut(s) | 738 |
| BssT1I | CCWWGG | 2 cut(s) | 414, 660 |
| Bst2UI | CCWGG | 1 cut(s) | 460 |
| Bst4CI | ACNGT | 1 cut(s) | 538 |
| Bst6I | CTCTTC | 1 cut(s) | 320 |
| BstACI | GRCGYC | 1 cut(s) | 738 |
| BstBI | TTCGAA | 1 cut(s) | 794 |
| BstDEI | CTNAG | 3 cut(s) | 66, 258, 988 |
| BstF5I | GGATG | 3 cut(s) | 268, 277, 1041 |
| BstHHI | GCGC | 1 cut(s) | 388 |
| BstKTI | GATC | 7 cut(s) | 295, 405, 424, 886, 944, 1022, 1026 |
| BstMBI | GATC | 7 cut(s) | 292, 402, 421, 883, 941, 1019, 1023 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 254, 617 |
| BstNI | CCWGG | 1 cut(s) | 460 |
| BstSCI | CCNGG | 1 cut(s) | 458 |
| BstX2I | RGATCY | 2 cut(s) | 421, 941 |
| BstYI | RGATCY | 2 cut(s) | 421, 941 |
| Bsu15I | ATCGAT | 1 cut(s) | 1022 |
| BsuI | GTATCC | 1 cut(s) | 421 |
| BsuRI | GGCC | 4 cut(s) | 298, 620, 639, 659 |
| BsuTUI | ATCGAT | 1 cut(s) | 1022 |
| BtsCI | GGATG | 3 cut(s) | 268, 277, 1041 |
| BtsI | GCAGTG | 1 cut(s) | 973 |
| BtsIMutI | CAGTG | 1 cut(s) | 973 |
| CciI | TCATGA | 1 cut(s) | 1052 |
| CfoI | GCGC | 1 cut(s) | 388 |
| Cfr13I | GGNCC | 1 cut(s) | 1046 |
| ClaI | ATCGAT | 1 cut(s) | 1022 |
| Csp6I | GTAC | 2 cut(s) | 283, 863 |
| CviAII | CATG | 4 cut(s) | 87, 492, 1010, 1053 |
| CviJI | RGCY | 9 cut(s) | 101, 115, 206, 248, 298, 620, 639, 659, 925 |
| CviKI_1 | RGCY | 9 cut(s) | 101, 115, 206, 248, 298, 620, 639, 659, 925 |
| CviQI | GTAC | 2 cut(s) | 283, 863 |
| DdeI | CTNAG | 3 cut(s) | 66, 258, 988 |
| DpnI | GATC | 7 cut(s) | 294, 404, 423, 885, 943, 1021, 1025 |
| DpnII | GATC | 7 cut(s) | 292, 402, 421, 883, 941, 1019, 1023 |
| EaeI | YGGCCR | 2 cut(s) | 618, 657 |
| Eam1104I | CTCTTC | 1 cut(s) | 320 |
| EarI | CTCTTC | 1 cut(s) | 320 |
| Eco130I | CCWWGG | 2 cut(s) | 414, 660 |
| Eco147I | AGGCCT | 1 cut(s) | 639 |
| Eco47I | GGWCC | 1 cut(s) | 1046 |
| Eco57I | CTGAAG | 1 cut(s) | 270 |
| Eco88I | CYCGRG | 1 cut(s) | 425 |
| EcoRI | GAATTC | 2 cut(s) | 74, 275 |
| EcoRII | CCWGG | 1 cut(s) | 458 |
| EcoT14I | CCWWGG | 2 cut(s) | 414, 660 |
| ErhI | CCWWGG | 2 cut(s) | 414, 660 |
| FaeI | CATG | 4 cut(s) | 90, 495, 1013, 1056 |
| FaqI | GGGAC | 3 cut(s) | 509, 773, 808 |
| FatI | CATG | 4 cut(s) | 86, 491, 1009, 1052 |
| FauNDI | CATATG | 2 cut(s) | 321, 759 |
| FokI | GGATG | 3 cut(s) | 275, 284, 1048 |
| FspBI | CTAG | 1 cut(s) | 452 |
| GlaI | GCGC | 1 cut(s) | 387 |
| HaeIII | GGCC | 4 cut(s) | 298, 620, 639, 659 |
| HhaI | GCGC | 1 cut(s) | 388 |
| Hin1I | GRCGYC | 1 cut(s) | 738 |
| Hin1II | CATG | 4 cut(s) | 90, 495, 1013, 1056 |
| Hin6I | GCGC | 1 cut(s) | 386 |
| HinP1I | GCGC | 1 cut(s) | 386 |
| HindIII | AAGCTT | 2 cut(s) | 113, 923 |
| HinfI | GANTC | 3 cut(s) | 122, 509, 584 |
| HphI | GGTGA | 2 cut(s) | 238, 563 |
| Hpy166II | GTNNAC | 1 cut(s) | 670 |
| Hpy188I | TCNGA | 7 cut(s) | 10, 110, 339, 399, 483, 644, 991 |
| Hpy188III | TCNNGA | 4 cut(s) | 425, 427, 887, 1053 |
| Hpy8I | GTNNAC | 1 cut(s) | 670 |
| Hpy99I | CGWCG | 1 cut(s) | 740 |
| HpyAV | CCTTC | 5 cut(s) | 28, 386, 537, 542, 801 |
| HpyCH4III | ACNGT | 1 cut(s) | 538 |
| HpyCH4IV | ACGT | 2 cut(s) | 738, 870 |
| HpyCH4V | TGCA | 3 cut(s) | 86, 368, 473 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 254, 617 |
| HpyF3I | CTNAG | 3 cut(s) | 66, 258, 988 |
| HpySE526I | ACGT | 2 cut(s) | 738, 870 |
| Hsp92I | GRCGYC | 1 cut(s) | 738 |
| Hsp92II | CATG | 4 cut(s) | 90, 495, 1013, 1056 |
| HspAI | GCGC | 1 cut(s) | 386 |
| Kzo9I | GATC | 7 cut(s) | 292, 402, 421, 883, 941, 1019, 1023 |
| LmnI | GCTCC | 1 cut(s) | 759 |
| LpnPI | CCDG | 5 cut(s) | 29, 194, 445, 472, 798 |
| LweI | GCATC | 5 cut(s) | 14, 244, 446, 460, 989 |
| MaeI | CTAG | 1 cut(s) | 452 |
| MaeII | ACGT | 2 cut(s) | 738, 870 |
| MaeIII | GTNAC | 2 cut(s) | 955, 968 |
| MalI | GATC | 7 cut(s) | 294, 404, 423, 885, 943, 1021, 1025 |
| MboI | GATC | 7 cut(s) | 292, 402, 421, 883, 941, 1019, 1023 |
| MboII | GAAGA | 7 cut(s) | 83, 241, 263, 337, 412, 806, 893 |
| MflI | RGATCY | 2 cut(s) | 421, 941 |
| MlsI | TGGCCA | 2 cut(s) | 620, 659 |
| MluNI | TGGCCA | 2 cut(s) | 620, 659 |
| MlyI | GAGTC | 3 cut(s) | 131, 503, 578 |
| MmeI | TCCRAC | 1 cut(s) | 520 |
| MnlI | CCTC | 8 cut(s) | 61, 211, 253, 423, 453, 629, 650, 670 |
| Mox20I | TGGCCA | 2 cut(s) | 620, 659 |
| MroXI | GAANNNNTTC | 1 cut(s) | 731 |
| MscI | TGGCCA | 2 cut(s) | 620, 659 |
| MseI | TTAA | 7 cut(s) | 117, 182, 570, 746, 828, 905, 927 |
| Msp20I | TGGCCA | 2 cut(s) | 620, 659 |
| MspR9I | CCNGG | 1 cut(s) | 460 |
| MvaI | CCWGG | 1 cut(s) | 460 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 254, 617 |
| NdeI | CATATG | 2 cut(s) | 321, 759 |
| NdeII | GATC | 7 cut(s) | 292, 402, 421, 883, 941, 1019, 1023 |
| NlaIII | CATG | 4 cut(s) | 90, 495, 1013, 1056 |
| NlaIV | GGNNCC | 1 cut(s) | 943 |
| NmuCI | GTSAC | 2 cut(s) | 955, 968 |
| NspV | TTCGAA | 1 cut(s) | 794 |
| PaeR7I | CTCGAG | 1 cut(s) | 425 |
| PagI | TCATGA | 1 cut(s) | 1052 |
| PceI | AGGCCT | 1 cut(s) | 639 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 867 |
| PdmI | GAANNNNTTC | 1 cut(s) | 731 |
| PflFI | GACNNNGTC | 1 cut(s) | 974 |
| PfoI | TCCNGGA | 1 cut(s) | 458 |
| PleI | GAGTC | 3 cut(s) | 130, 503, 578 |
| PpsI | GAGTC | 3 cut(s) | 130, 503, 578 |
| Psp6I | CCWGG | 1 cut(s) | 458 |
| PspGI | CCWGG | 1 cut(s) | 458 |
| PspN4I | GGNNCC | 1 cut(s) | 943 |
| PspPI | GGNCC | 1 cut(s) | 1046 |
| PsuI | RGATCY | 2 cut(s) | 421, 941 |
| PsyI | GACNNNGTC | 1 cut(s) | 974 |
| RsaI | GTAC | 2 cut(s) | 284, 864 |
| RsaNI | GTAC | 2 cut(s) | 283, 863 |
| SaqAI | TTAA | 7 cut(s) | 117, 182, 570, 746, 828, 905, 927 |
| Sau3AI | GATC | 7 cut(s) | 292, 402, 421, 883, 941, 1019, 1023 |
| Sau96I | GGNCC | 1 cut(s) | 1046 |
| SchI | GAGTC | 3 cut(s) | 131, 503, 578 |
| ScrFI | CCNGG | 1 cut(s) | 460 |
| SfaNI | GCATC | 5 cut(s) | 14, 244, 446, 460, 989 |
| Sfr274I | CTCGAG | 1 cut(s) | 425 |
| SfuI | TTCGAA | 1 cut(s) | 794 |
| SinI | GGWCC | 1 cut(s) | 1046 |
| SlaI | CTCGAG | 1 cut(s) | 425 |
| SmlI | CTYRAG | 1 cut(s) | 425 |
| SmoI | CTYRAG | 1 cut(s) | 425 |
| SpeI | ACTAGT | 1 cut(s) | 451 |
| SseBI | AGGCCT | 1 cut(s) | 639 |
| SsiI | CCGC | 2 cut(s) | 611, 665 |
| SspI | AATATT | 1 cut(s) | 607 |
| SspMI | CTAG | 1 cut(s) | 452 |
| StuI | AGGCCT | 1 cut(s) | 639 |
| StyD4I | CCNGG | 1 cut(s) | 458 |
| StyI | CCWWGG | 2 cut(s) | 414, 660 |
| TaaI | ACNGT | 1 cut(s) | 538 |
| TaiI | ACGT | 2 cut(s) | 741, 873 |
| TaqI | TCGA | 7 cut(s) | 306, 426, 560, 725, 735, 794, 1022 |
| TaqII | GACCGA | 1 cut(s) | 596 |
| Tru1I | TTAA | 7 cut(s) | 117, 182, 570, 746, 828, 905, 927 |
| Tru9I | TTAA | 7 cut(s) | 117, 182, 570, 746, 828, 905, 927 |
| TscAI | CASTG | 1 cut(s) | 973 |
| TseFI | GTSAC | 2 cut(s) | 955, 968 |
| Tsp45I | GTSAC | 2 cut(s) | 955, 968 |
| TspDTI | ATGAA | 7 cut(s) | 182, 288, 338, 543, 894, 1026, 1052 |
| TspRI | CASTG | 1 cut(s) | 973 |
| Tth111I | GACNNNGTC | 1 cut(s) | 974 |
| VpaK11BI | GGWCC | 1 cut(s) | 1046 |
| XapI | RAATTY | 5 cut(s) | 11, 74, 275, 393, 691 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 628 |
| XhoI | CTCGAG | 1 cut(s) | 425 |
| XmnI | GAANNNNTTC | 1 cut(s) | 731 |
| XspI | CTAG | 1 cut(s) | 452 |
| ZraI | GACGTC | 1 cut(s) | 739 |