disease resistance
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1647 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1521 |
| Acc16I | TGCGCA | 1 cut(s) | 2511 |
| Acc36I | ACCTGC | 1 cut(s) | 1647 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1115 |
| AccI | GTMKAC | 3 cut(s) | 931, 2080, 2230 |
| AccII | CGCG | 1 cut(s) | 1764 |
| AciI | CCGC | 2 cut(s) | 1764, 2342 |
| AclWI | GGATC | 1 cut(s) | 1186 |
| AcoI | YGGCCR | 1 cut(s) | 1117 |
| AcsI | RAATTY | 5 cut(s) | 298, 658, 999, 1666, 2096 |
| AcuI | CTGAAG | 4 cut(s) | 1430, 1622, 1904, 2477 |
| AfaI | GTAC | 9 cut(s) | 63, 369, 695, 966, 1107, 1622, 1759, 1981, 2531 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1115 |
| AflII | CTTAAG | 1 cut(s) | 1142 |
| AflIII | ACRYGT | 4 cut(s) | 385, 469, 676, 2668 |
| AjnI | CCWGG | 1 cut(s) | 451 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 863, 895, 2274, 2306 |
| AleI | CACNNNNGTG | 1 cut(s) | 2198 |
| AluBI | AGCT | 8 cut(s) | 1220, 1244, 1354, 1775, 1784, 1941, 2179, 2311 |
| AluI | AGCT | 8 cut(s) | 1220, 1244, 1354, 1775, 1784, 1941, 2179, 2311 |
| Alw21I | GWGCWC | 3 cut(s) | 446, 1390, 1469 |
| Alw26I | GTCTC | 4 cut(s) | 1229, 1772, 1979, 2128 |
| AlwI | GGATC | 1 cut(s) | 1186 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1220 |
| Ama87I | CYCGRG | 3 cut(s) | 1361, 1874, 2312 |
| AoxI | GGCC | 8 cut(s) | 973, 1117, 1281, 1687, 1834, 2372, 2629, 2639 |
| ApeKI | GCWGC | 5 cut(s) | 1626, 1941, 2246, 2262, 2554 |
| ApoI | RAATTY | 5 cut(s) | 298, 658, 999, 1666, 2096 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1228, 1260 |
| AseI | ATTAAT | 1 cut(s) | 1793 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2400 |
| AspA2I | CCTAGG | 1 cut(s) | 779 |
| AspLEI | GCGC | 3 cut(s) | 9, 1312, 2512 |
| AspS9I | GGNCC | 3 cut(s) | 776, 2630, 2742 |
| AsuHPI | GGTGA | 5 cut(s) | 218, 1432, 2212, 2260, 2554 |
| AsuII | TTCGAA | 1 cut(s) | 422 |
| AsuNHI | GCTAGC | 1 cut(s) | 1051 |
| AvaI | CYCGRG | 3 cut(s) | 1361, 1874, 2312 |
| AvaII | GGWCC | 2 cut(s) | 776, 2742 |
| AvrII | CCTAGG | 1 cut(s) | 779 |
| BalI | TGGCCA | 1 cut(s) | 1119 |
| BauI | CACGAG | 1 cut(s) | 1239 |
| BbsI | GAAGAC | 2 cut(s) | 438, 2700 |
| Bbv12I | GWGCWC | 3 cut(s) | 446, 1390, 1469 |
| BbvI | GCAGC | 5 cut(s) | 1613, 1928, 2258, 2274, 2566 |
| BceAI | ACGGC | 2 cut(s) | 835, 1702 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2665, 2699 |
| BciT130I | CCWGG | 1 cut(s) | 453 |
| BclI | TGATCA | 1 cut(s) | 1198 |
| BcoDI | GTCTC | 4 cut(s) | 1229, 1772, 1979, 2128 |
| BfmI | CTRYAG | 4 cut(s) | 605, 1369, 2001, 2260 |
| BfoI | RGCGCY | 1 cut(s) | 1313 |
| BfrI | CTTAAG | 1 cut(s) | 1142 |
| BfuAI | ACCTGC | 1 cut(s) | 1647 |
| BglII | AGATCT | 2 cut(s) | 1576, 2583 |
| BisI | GCNGC | 5 cut(s) | 1627, 1942, 2247, 2263, 2555 |
| BlnI | CCTAGG | 1 cut(s) | 779 |
| BlsI | GCNGC | 5 cut(s) | 1628, 1943, 2248, 2264, 2556 |
| BmcAI | AGTACT | 1 cut(s) | 1622 |
| Bme1390I | CCNGG | 1 cut(s) | 453 |
| Bme18I | GGWCC | 2 cut(s) | 776, 2742 |
| BmeT110I | CYCGRG | 3 cut(s) | 1361, 1874, 2312 |
| BmgT120I | GGNCC | 3 cut(s) | 776, 2630, 2742 |
| BmiI | GGNNCC | 3 cut(s) | 705, 777, 2738 |
| BmrFI | CCNGG | 1 cut(s) | 453 |
| BmsI | GCATC | 6 cut(s) | 345, 1201, 1790, 2258, 2495, 2603 |
| BmtI | GCTAGC | 1 cut(s) | 1055 |
| BpiI | GAAGAC | 2 cut(s) | 438, 2700 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1372, 1404 |
| BpmI | CTGGAG | 1 cut(s) | 1832 |
| Bpu14I | TTCGAA | 1 cut(s) | 422 |
| BpuEI | CTTGAG | 3 cut(s) | 198, 556, 1367 |
| BsaAI | YACGTR | 2 cut(s) | 470, 697 |
| BsaBI | GATNNNNATC | 1 cut(s) | 31 |
| BsaI | GGTCTC | 1 cut(s) | 1772 |
| BsaJI | CCNNGG | 6 cut(s) | 644, 779, 1510, 1609, 2619, 2757 |
| BsaWI | WCCGGW | 2 cut(s) | 1316, 2739 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1115 |
| Bse118I | RCCGGY | 1 cut(s) | 405 |
| Bse1I | ACTGG | 4 cut(s) | 923, 1448, 1905, 2271 |
| Bse3DI | GCAATG | 2 cut(s) | 446, 2512 |
| Bse8I | GATNNNNATC | 1 cut(s) | 31 |
| BseBI | CCWGG | 1 cut(s) | 453 |
| BseDI | CCNNGG | 6 cut(s) | 644, 779, 1510, 1609, 2619, 2757 |
| BseJI | GATNNNNATC | 1 cut(s) | 31 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1115 |
| BseMI | GCAATG | 2 cut(s) | 446, 2512 |
| BseMII | CTCAG | 4 cut(s) | 1454, 1507, 1990, 2268 |
| BseNI | ACTGG | 4 cut(s) | 923, 1448, 1905, 2271 |
| BseRI | GAGGAG | 3 cut(s) | 1292, 1364, 2636 |
| BseXI | GCAGC | 5 cut(s) | 1613, 1928, 2258, 2274, 2566 |
| BseYI | CCCAGC | 1 cut(s) | 2473 |
| BsgI | GTGCAG | 1 cut(s) | 1894 |
| Bsh1236I | CGCG | 1 cut(s) | 1764 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2064 |
| BshFI | GGCC | 8 cut(s) | 975, 1119, 1283, 1689, 1836, 2374, 2631, 2641 |
| BsiEI | CGRYCG | 1 cut(s) | 2064 |
| BsiHKAI | GWGCWC | 3 cut(s) | 446, 1390, 1469 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1361, 1874, 2312 |
| BsiSI | CCGG | 4 cut(s) | 406, 1317, 1529, 2740 |
| BslFI | GGGAC | 3 cut(s) | 71, 1442, 1857 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1115 |
| BsmAI | GTCTC | 4 cut(s) | 1229, 1772, 1979, 2128 |
| BsmBI | CGTCTC | 1 cut(s) | 2128 |
| BsmFI | GGGAC | 3 cut(s) | 71, 1442, 1857 |
| BsmI | GAATGC | 2 cut(s) | 1091, 1458 |
| BsnI | GGCC | 8 cut(s) | 975, 1119, 1283, 1689, 1836, 2374, 2631, 2641 |
| Bso31I | GGTCTC | 1 cut(s) | 1772 |
| BsoBI | CYCGRG | 3 cut(s) | 1361, 1874, 2312 |
| Bsp119I | TTCGAA | 1 cut(s) | 422 |
| Bsp1286I | GDGCHC | 3 cut(s) | 446, 1390, 1469 |
| Bsp1407I | TGTACA | 1 cut(s) | 367 |
| Bsp143I | GATC | 8 cut(s) | 361, 740, 1146, 1178, 1198, 1576, 2061, 2583 |
| BspACI | CCGC | 2 cut(s) | 1764, 2342 |
| BspANI | GGCC | 8 cut(s) | 975, 1119, 1283, 1689, 1836, 2374, 2631, 2641 |
| BspCNI | CTCAG | 4 cut(s) | 1455, 1506, 1989, 2267 |
| BspFNI | CGCG | 1 cut(s) | 1764 |
| BspHI | TCATGA | 1 cut(s) | 552 |
| BspLI | GGNNCC | 3 cut(s) | 705, 777, 2738 |
| BspMAI | CTGCAG | 1 cut(s) | 2264 |
| BspMI | ACCTGC | 1 cut(s) | 1647 |
| BspOI | GCTAGC | 1 cut(s) | 1055 |
| BspPI | GGATC | 1 cut(s) | 1186 |
| BspT104I | TTCGAA | 1 cut(s) | 422 |
| BspTI | CTTAAG | 1 cut(s) | 1142 |
| BspTNI | GGTCTC | 1 cut(s) | 1772 |
| BsrDI | GCAATG | 2 cut(s) | 446, 2512 |
| BsrFI | RCCGGY | 1 cut(s) | 405 |
| BsrGI | TGTACA | 1 cut(s) | 367 |
| BsrI | ACTGG | 4 cut(s) | 923, 1448, 1905, 2271 |
| BssAI | RCCGGY | 1 cut(s) | 405 |
| BssECI | CCNNGG | 6 cut(s) | 644, 779, 1510, 1609, 2619, 2757 |
| BssMI | GATC | 8 cut(s) | 361, 740, 1146, 1178, 1198, 1576, 2061, 2583 |
| BssSI | CACGAG | 1 cut(s) | 1239 |
| BssT1I | CCWWGG | 4 cut(s) | 779, 1510, 1609, 2757 |
| Bst2BI | CACGAG | 1 cut(s) | 1239 |
| Bst2UI | CCWGG | 1 cut(s) | 453 |
| Bst4CI | ACNGT | 4 cut(s) | 417, 1370, 2016, 2085 |
| Bst6I | CTCTTC | 7 cut(s) | 1315, 1406, 1641, 1923, 2115, 2496, 2604 |
| BstAFI | CTTAAG | 1 cut(s) | 1142 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1095 |
| BstAUI | TGTACA | 1 cut(s) | 367 |
| BstBAI | YACGTR | 2 cut(s) | 470, 697 |
| BstBI | TTCGAA | 1 cut(s) | 422 |
| BstC8I | GCNNGC | 5 cut(s) | 1053, 1057, 1157, 1445, 1691 |
| BstFNI | CGCG | 1 cut(s) | 1764 |
| BstH2I | RGCGCY | 1 cut(s) | 1313 |
| BstHHI | GCGC | 3 cut(s) | 9, 1312, 2512 |
| BstKTI | GATC | 8 cut(s) | 364, 743, 1149, 1181, 1201, 1579, 2064, 2586 |
| BstMAI | GTCTC | 4 cut(s) | 1229, 1772, 1979, 2128 |
| BstMBI | GATC | 8 cut(s) | 361, 740, 1146, 1178, 1198, 1576, 2061, 2583 |
| BstMCI | CGRYCG | 1 cut(s) | 2064 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 872, 1095, 1464, 1781 |
| BstNI | CCWGG | 1 cut(s) | 453 |
| BstNSI | RCATGY | 5 cut(s) | 389, 680, 1159, 1447, 2672 |
| BstSCI | CCNGG | 1 cut(s) | 451 |
| BstSFI | CTRYAG | 4 cut(s) | 605, 1369, 2001, 2260 |
| BstUI | CGCG | 1 cut(s) | 1764 |
| BstV1I | GCAGC | 5 cut(s) | 1613, 1928, 2258, 2274, 2566 |
| BstV2I | GAAGAC | 2 cut(s) | 438, 2700 |
| BstX2I | RGATCY | 3 cut(s) | 1178, 1576, 2583 |
| BstYI | RGATCY | 3 cut(s) | 1178, 1576, 2583 |
| BsuRI | GGCC | 8 cut(s) | 975, 1119, 1283, 1689, 1836, 2374, 2631, 2641 |
| BtsIMutI | CAGTG | 2 cut(s) | 413, 1650 |
| BveI | ACCTGC | 1 cut(s) | 1647 |
| Cac8I | GCNNGC | 5 cut(s) | 1053, 1057, 1157, 1445, 1691 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1220 |
| CciI | TCATGA | 1 cut(s) | 552 |
| CfoI | GCGC | 3 cut(s) | 9, 1312, 2512 |
| Cfr10I | RCCGGY | 1 cut(s) | 405 |
| Cfr13I | GGNCC | 3 cut(s) | 776, 2630, 2742 |
| Csp6I | GTAC | 9 cut(s) | 62, 368, 694, 965, 1106, 1621, 1758, 1980, 2530 |
| CviQI | GTAC | 9 cut(s) | 62, 368, 694, 965, 1106, 1621, 1758, 1980, 2530 |
| DpnI | GATC | 8 cut(s) | 363, 742, 1148, 1180, 1200, 1578, 2063, 2585 |
| DpnII | GATC | 8 cut(s) | 361, 740, 1146, 1178, 1198, 1576, 2061, 2583 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1521 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1521 |
| EaeI | YGGCCR | 1 cut(s) | 1117 |
| Eam1104I | CTCTTC | 7 cut(s) | 1315, 1406, 1641, 1923, 2115, 2496, 2604 |
| EarI | CTCTTC | 7 cut(s) | 1315, 1406, 1641, 1923, 2115, 2496, 2604 |
| Eco130I | CCWWGG | 4 cut(s) | 779, 1510, 1609, 2757 |
| Eco147I | AGGCCT | 2 cut(s) | 975, 1836 |
| Eco31I | GGTCTC | 1 cut(s) | 1772 |
| Eco32I | GATATC | 2 cut(s) | 1507, 1600 |
| Eco47I | GGWCC | 2 cut(s) | 776, 2742 |
| Eco57I | CTGAAG | 4 cut(s) | 1430, 1622, 1904, 2477 |
| Eco88I | CYCGRG | 3 cut(s) | 1361, 1874, 2312 |
| EcoO109I | RGGNCCY | 1 cut(s) | 776 |
| EcoRII | CCWGG | 1 cut(s) | 451 |
| EcoRV | GATATC | 2 cut(s) | 1507, 1600 |
| EcoT14I | CCWWGG | 4 cut(s) | 779, 1510, 1609, 2757 |
| ErhI | CCWWGG | 4 cut(s) | 779, 1510, 1609, 2757 |
| Esp3I | CGTCTC | 1 cut(s) | 2128 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 176, 208, 207, 239 |
| FaqI | GGGAC | 3 cut(s) | 71, 1442, 1857 |
| FbaI | TGATCA | 1 cut(s) | 1198 |
| FblI | GTMKAC | 3 cut(s) | 931, 2080, 2230 |
| Fnu4HI | GCNGC | 5 cut(s) | 1627, 1942, 2247, 2263, 2555 |
| Fsp4HI | GCNGC | 5 cut(s) | 1627, 1942, 2247, 2263, 2555 |
| FspI | TGCGCA | 1 cut(s) | 2511 |
| GlaI | GCGC | 3 cut(s) | 8, 1311, 2511 |
| GluI | GCNGC | 5 cut(s) | 1627, 1942, 2247, 2263, 2555 |
| GsaI | CCCAGC | 1 cut(s) | 2477 |
| GsuI | CTGGAG | 1 cut(s) | 1832 |
| HaeII | RGCGCY | 1 cut(s) | 1313 |
| HaeIII | GGCC | 8 cut(s) | 975, 1119, 1283, 1689, 1836, 2374, 2631, 2641 |
| HapII | CCGG | 4 cut(s) | 406, 1317, 1529, 2740 |
| HhaI | GCGC | 3 cut(s) | 9, 1312, 2512 |
| Hin6I | GCGC | 3 cut(s) | 7, 1310, 2510 |
| HinP1I | GCGC | 3 cut(s) | 7, 1310, 2510 |
| HincII | GTYRAC | 6 cut(s) | 593, 667, 909, 949, 1998, 2081 |
| HindII | GTYRAC | 6 cut(s) | 593, 667, 909, 949, 1998, 2081 |
| HindIII | AAGCTT | 1 cut(s) | 1782 |
| HpaI | GTTAAC | 1 cut(s) | 909 |
| HpaII | CCGG | 4 cut(s) | 406, 1317, 1529, 2740 |
| HphI | GGTGA | 5 cut(s) | 218, 1432, 2212, 2260, 2554 |
| HpyAV | CCTTC | 9 cut(s) | 418, 759, 766, 965, 1094, 1526, 2060, 2198, 2272 |
| HpyCH4III | ACNGT | 4 cut(s) | 417, 1370, 2016, 2085 |
| HpyCH4IV | ACGT | 5 cut(s) | 60, 469, 696, 1203, 2195 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 872, 1095, 1464, 1781 |
| HpySE526I | ACGT | 5 cut(s) | 60, 469, 696, 1203, 2195 |
| HspAI | GCGC | 3 cut(s) | 7, 1310, 2510 |
| Ksp22I | TGATCA | 1 cut(s) | 1198 |
| KspAI | GTTAAC | 1 cut(s) | 909 |
| Kzo9I | GATC | 8 cut(s) | 361, 740, 1146, 1178, 1198, 1576, 2061, 2583 |
| LmnI | GCTCC | 4 cut(s) | 1351, 1393, 1808, 2742 |
| Lsp1109I | GCAGC | 5 cut(s) | 1613, 1928, 2258, 2274, 2566 |
| LweI | GCATC | 6 cut(s) | 345, 1201, 1790, 2258, 2495, 2603 |
| MaeII | ACGT | 5 cut(s) | 60, 469, 696, 1203, 2195 |
| MaeIII | GTNAC | 4 cut(s) | 316, 1027, 1951, 2671 |
| MalI | GATC | 8 cut(s) | 363, 742, 1148, 1180, 1200, 1578, 2063, 2585 |
| MboI | GATC | 8 cut(s) | 361, 740, 1146, 1178, 1198, 1576, 2061, 2583 |
| MfeI | CAATTG | 1 cut(s) | 1093 |
| MflI | RGATCY | 3 cut(s) | 1178, 1576, 2583 |
| MhlI | GDGCHC | 3 cut(s) | 446, 1390, 1469 |
| MlsI | TGGCCA | 1 cut(s) | 1119 |
| MluNI | TGGCCA | 1 cut(s) | 1119 |
| MlyI | GAGTC | 4 cut(s) | 506, 573, 1351, 2324 |
| MmeI | TCCRAC | 3 cut(s) | 832, 1003, 1847 |
| Mox20I | TGGCCA | 1 cut(s) | 1119 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2400 |
| MscI | TGGCCA | 1 cut(s) | 1119 |
| MslI | CAYNNNNRTG | 3 cut(s) | 633, 1188, 2198 |
| Msp20I | TGGCCA | 1 cut(s) | 1119 |
| MspCI | CTTAAG | 1 cut(s) | 1142 |
| MspI | CCGG | 4 cut(s) | 406, 1317, 1529, 2740 |
| MspR9I | CCNGG | 1 cut(s) | 453 |
| MunI | CAATTG | 1 cut(s) | 1093 |
| Mva1269I | GAATGC | 2 cut(s) | 1091, 1458 |
| MvaI | CCWGG | 1 cut(s) | 453 |
| MvnI | CGCG | 1 cut(s) | 1764 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 872, 1095, 1464, 1781 |
| NdeII | GATC | 8 cut(s) | 361, 740, 1146, 1178, 1198, 1576, 2061, 2583 |
| NheI | GCTAGC | 1 cut(s) | 1051 |
| NlaIV | GGNNCC | 3 cut(s) | 705, 777, 2738 |
| NmuCI | GTSAC | 1 cut(s) | 2671 |
| NsbI | TGCGCA | 1 cut(s) | 2511 |
| NspI | RCATGY | 5 cut(s) | 389, 680, 1159, 1447, 2672 |
| NspV | TTCGAA | 1 cut(s) | 422 |
| OliI | CACNNNNGTG | 1 cut(s) | 2198 |
| PaeI | GCATGC | 2 cut(s) | 1159, 1447 |
| PaeR7I | CTCGAG | 2 cut(s) | 1361, 2312 |
| PagI | TCATGA | 1 cut(s) | 552 |
| PaqCI | CACCTGC | 1 cut(s) | 1647 |
| PceI | AGGCCT | 2 cut(s) | 975, 1836 |
| PciI | ACATGT | 3 cut(s) | 385, 676, 2668 |
| PctI | GAATGC | 2 cut(s) | 1091, 1458 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2400 |
| PfeI | GAWTC | 6 cut(s) | 81, 196, 326, 1555, 1904, 2647 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1115 |
| PkrI | GCNGC | 5 cut(s) | 1628, 1943, 2248, 2264, 2556 |
| Ple19I | CGATCG | 1 cut(s) | 2064 |
| PleI | GAGTC | 4 cut(s) | 506, 572, 1351, 2323 |
| PpsI | GAGTC | 4 cut(s) | 506, 572, 1351, 2323 |
| Ppu21I | YACGTR | 2 cut(s) | 470, 697 |
| PpuMI | RGGWCCY | 1 cut(s) | 776 |
| PscI | ACATGT | 3 cut(s) | 385, 676, 2668 |
| PshBI | ATTAAT | 1 cut(s) | 1793 |
| Psp5II | RGGWCCY | 1 cut(s) | 776 |
| Psp6I | CCWGG | 1 cut(s) | 451 |
| PspFI | CCCAGC | 1 cut(s) | 2473 |
| PspGI | CCWGG | 1 cut(s) | 451 |
| PspN4I | GGNNCC | 3 cut(s) | 705, 777, 2738 |
| PspPI | GGNCC | 3 cut(s) | 776, 2630, 2742 |
| PspPPI | RGGWCCY | 1 cut(s) | 776 |
| PspXI | VCTCGAGB | 1 cut(s) | 2312 |
| PstI | CTGCAG | 1 cut(s) | 2264 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1220 |
| PsuI | RGATCY | 3 cut(s) | 1178, 1576, 2583 |
| PvuI | CGATCG | 1 cut(s) | 2064 |
| RsaI | GTAC | 9 cut(s) | 63, 369, 695, 966, 1107, 1622, 1759, 1981, 2531 |
| RsaNI | GTAC | 9 cut(s) | 62, 368, 694, 965, 1106, 1621, 1758, 1980, 2530 |
| RseI | CAYNNNNRTG | 3 cut(s) | 633, 1188, 2198 |
| SalI | GTCGAC | 1 cut(s) | 2079 |
| SatI | GCNGC | 5 cut(s) | 1627, 1942, 2247, 2263, 2555 |
| Sau3AI | GATC | 8 cut(s) | 361, 740, 1146, 1178, 1198, 1576, 2061, 2583 |
| Sau96I | GGNCC | 3 cut(s) | 776, 2630, 2742 |
| ScaI | AGTACT | 1 cut(s) | 1622 |
| SchI | GAGTC | 4 cut(s) | 506, 573, 1351, 2324 |
| ScrFI | CCNGG | 1 cut(s) | 453 |
| SduI | GDGCHC | 3 cut(s) | 446, 1390, 1469 |
| SfaNI | GCATC | 6 cut(s) | 345, 1201, 1790, 2258, 2495, 2603 |
| SfcI | CTRYAG | 4 cut(s) | 605, 1369, 2001, 2260 |
| Sfr274I | CTCGAG | 2 cut(s) | 1361, 2312 |
| SfuI | TTCGAA | 1 cut(s) | 422 |
| SinI | GGWCC | 2 cut(s) | 776, 2742 |
| SlaI | CTCGAG | 2 cut(s) | 1361, 2312 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 633, 1188, 2198 |
| SmlI | CTYRAG | 6 cut(s) | 213, 535, 1142, 1346, 1361, 2312 |
| SmoI | CTYRAG | 6 cut(s) | 213, 535, 1142, 1346, 1361, 2312 |
| SphI | GCATGC | 2 cut(s) | 1159, 1447 |
| SseBI | AGGCCT | 2 cut(s) | 975, 1836 |
| SsiI | CCGC | 2 cut(s) | 1764, 2342 |
| SspI | AATATT | 2 cut(s) | 278, 340 |
| StuI | AGGCCT | 2 cut(s) | 975, 1836 |
| StyD4I | CCNGG | 1 cut(s) | 451 |
| StyI | CCWWGG | 4 cut(s) | 779, 1510, 1609, 2757 |
| TaaI | ACNGT | 4 cut(s) | 417, 1370, 2016, 2085 |
| TaiI | ACGT | 5 cut(s) | 63, 472, 699, 1206, 2198 |
| TaqII | GACCGA | 1 cut(s) | 578 |
| TatI | WGTACW | 5 cut(s) | 367, 964, 1620, 1757, 1979 |
| TfiI | GAWTC | 6 cut(s) | 81, 196, 326, 1555, 1904, 2647 |
| TscAI | CASTG | 2 cut(s) | 420, 1650 |
| TseFI | GTSAC | 1 cut(s) | 2671 |
| TseI | GCWGC | 5 cut(s) | 1626, 1941, 2246, 2262, 2554 |
| Tsp45I | GTSAC | 1 cut(s) | 2671 |
| TspDTI | ATGAA | 8 cut(s) | 126, 151, 288, 569, 1009, 1735, 2540, 2693 |
| TspGWI | ACGGA | 1 cut(s) | 2039 |
| TspRI | CASTG | 2 cut(s) | 420, 1650 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1115 |
| Vha464I | CTTAAG | 1 cut(s) | 1142 |
| VpaK11BI | GGWCC | 2 cut(s) | 776, 2742 |
| VspI | ATTAAT | 1 cut(s) | 1793 |
| XapI | RAATTY | 5 cut(s) | 298, 658, 999, 1666, 2096 |
| XbaI | TCTAGA | 2 cut(s) | 250, 1738 |
| XceI | RCATGY | 5 cut(s) | 389, 680, 1159, 1447, 2672 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1291 |
| XhoI | CTCGAG | 2 cut(s) | 1361, 2312 |
| XmaJI | CCTAGG | 1 cut(s) | 779 |
| XmiI | GTMKAC | 3 cut(s) | 931, 2080, 2230 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2400 |
| ZrmI | AGTACT | 1 cut(s) | 1622 |