ADP binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 807 |
| AciI | CCGC | 1 cut(s) | 112 |
| AclWI | GGATC | 1 cut(s) | 743 |
| AcsI | RAATTY | 2 cut(s) | 67, 391 |
| AfaI | GTAC | 4 cut(s) | 248, 279, 296, 1116 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 82, 133, 712 |
| AgsI | TTSAA | 7 cut(s) | 91, 104, 209, 284, 397, 561, 814 |
| AjnI | CCWGG | 1 cut(s) | 27 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 614, 646 |
| AluBI | AGCT | 8 cut(s) | 218, 410, 526, 611, 953, 1037, 1078, 1192 |
| AluI | AGCT | 8 cut(s) | 218, 410, 526, 611, 953, 1037, 1078, 1192 |
| Alw26I | GTCTC | 1 cut(s) | 962 |
| AlwI | GGATC | 1 cut(s) | 743 |
| ApeKI | GCWGC | 3 cut(s) | 23, 407, 953 |
| ApoI | RAATTY | 2 cut(s) | 67, 391 |
| AsuHPI | GGTGA | 3 cut(s) | 208, 400, 727 |
| AsuNHI | GCTAGC | 1 cut(s) | 410 |
| AxyI | CCTNAGG | 1 cut(s) | 177 |
| BanII | GRGCYC | 1 cut(s) | 175 |
| BbsI | GAAGAC | 2 cut(s) | 216, 629 |
| BbvI | GCAGC | 3 cut(s) | 10, 419, 940 |
| BccI | CCATC | 1 cut(s) | 586 |
| BceAI | ACGGC | 1 cut(s) | 281 |
| BciT130I | CCWGG | 1 cut(s) | 29 |
| BcoDI | GTCTC | 1 cut(s) | 962 |
| BfaI | CTAG | 5 cut(s) | 411, 572, 636, 839, 1193 |
| BfuAI | ACCTGC | 1 cut(s) | 807 |
| BisI | GCNGC | 3 cut(s) | 24, 408, 954 |
| BlsI | GCNGC | 3 cut(s) | 25, 409, 955 |
| Bme1390I | CCNGG | 1 cut(s) | 29 |
| BmiI | GGNNCC | 1 cut(s) | 174 |
| BmrFI | CCNGG | 1 cut(s) | 29 |
| BmsI | GCATC | 4 cut(s) | 139, 214, 445, 772 |
| BmtI | GCTAGC | 1 cut(s) | 414 |
| BpiI | GAAGAC | 2 cut(s) | 216, 629 |
| BsaJI | CCNNGG | 4 cut(s) | 27, 28, 384, 913 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 515, 545 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 82, 133, 712 |
| Bse1I | ACTGG | 1 cut(s) | 1087 |
| Bse21I | CCTNAGG | 1 cut(s) | 177 |
| BseBI | CCWGG | 1 cut(s) | 29 |
| BseDI | CCNNGG | 4 cut(s) | 27, 28, 384, 913 |
| BseGI | GGATG | 3 cut(s) | 247, 787, 880 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 82, 133, 712 |
| BseMII | CTCAG | 1 cut(s) | 168 |
| BseNI | ACTGG | 1 cut(s) | 1087 |
| BseXI | GCAGC | 3 cut(s) | 10, 419, 940 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 82, 133, 712 |
| BsmAI | GTCTC | 1 cut(s) | 962 |
| BsmBI | CGTCTC | 1 cut(s) | 962 |
| Bsp1286I | GDGCHC | 1 cut(s) | 175 |
| Bsp1407I | TGTACA | 1 cut(s) | 294 |
| Bsp143I | GATC | 3 cut(s) | 166, 638, 735 |
| BspACI | CCGC | 1 cut(s) | 112 |
| BspCNI | CTCAG | 1 cut(s) | 169 |
| BspHI | TCATGA | 3 cut(s) | 220, 445, 649 |
| BspLI | GGNNCC | 1 cut(s) | 174 |
| BspMI | ACCTGC | 1 cut(s) | 807 |
| BspOI | GCTAGC | 1 cut(s) | 414 |
| BspPI | GGATC | 1 cut(s) | 743 |
| BsrGI | TGTACA | 1 cut(s) | 294 |
| BsrI | ACTGG | 1 cut(s) | 1087 |
| BssECI | CCNNGG | 4 cut(s) | 27, 28, 384, 913 |
| BssMI | GATC | 3 cut(s) | 166, 638, 735 |
| BssT1I | CCWWGG | 2 cut(s) | 384, 913 |
| Bst2UI | CCWGG | 1 cut(s) | 29 |
| Bst4CI | ACNGT | 1 cut(s) | 656 |
| BstAUI | TGTACA | 1 cut(s) | 294 |
| BstC8I | GCNNGC | 1 cut(s) | 412 |
| BstDEI | CTNAG | 3 cut(s) | 177, 430, 728 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 131 |
| BstF5I | GGATG | 3 cut(s) | 247, 787, 880 |
| BstKTI | GATC | 3 cut(s) | 169, 641, 738 |
| BstMAI | GTCTC | 1 cut(s) | 962 |
| BstMBI | GATC | 3 cut(s) | 166, 638, 735 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 56, 224 |
| BstNI | CCWGG | 1 cut(s) | 29 |
| BstSCI | CCNGG | 1 cut(s) | 27 |
| BstV1I | GCAGC | 3 cut(s) | 10, 419, 940 |
| BstV2I | GAAGAC | 2 cut(s) | 216, 629 |
| BstX2I | RGATCY | 1 cut(s) | 735 |
| BstYI | RGATCY | 1 cut(s) | 735 |
| Bsu36I | CCTNAGG | 1 cut(s) | 177 |
| BtsCI | GGATG | 3 cut(s) | 247, 787, 880 |
| BveI | ACCTGC | 1 cut(s) | 807 |
| Cac8I | GCNNGC | 1 cut(s) | 412 |
| CciI | TCATGA | 3 cut(s) | 220, 445, 649 |
| CseI | GACGC | 1 cut(s) | 979 |
| Csp6I | GTAC | 4 cut(s) | 247, 278, 295, 1115 |
| CviAII | CATG | 5 cut(s) | 221, 352, 446, 650, 1060 |
| CviQI | GTAC | 4 cut(s) | 247, 278, 295, 1115 |
| DdeI | CTNAG | 3 cut(s) | 177, 430, 728 |
| DpnI | GATC | 3 cut(s) | 168, 640, 737 |
| DpnII | GATC | 3 cut(s) | 166, 638, 735 |
| Eco130I | CCWWGG | 2 cut(s) | 384, 913 |
| Eco24I | GRGCYC | 1 cut(s) | 175 |
| Eco32I | GATATC | 1 cut(s) | 827 |
| Eco81I | CCTNAGG | 1 cut(s) | 177 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 131 |
| EcoRII | CCWGG | 1 cut(s) | 27 |
| EcoRV | GATATC | 1 cut(s) | 827 |
| EcoT14I | CCWWGG | 2 cut(s) | 384, 913 |
| EcoT38I | GRGCYC | 1 cut(s) | 175 |
| ErhI | CCWWGG | 2 cut(s) | 384, 913 |
| Esp3I | CGTCTC | 1 cut(s) | 962 |
| FaeI | CATG | 5 cut(s) | 224, 355, 449, 653, 1063 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 34, 66, 84, 116 |
| FatI | CATG | 5 cut(s) | 220, 351, 445, 649, 1059 |
| FauI | CCCGC | 1 cut(s) | 105 |
| Fnu4HI | GCNGC | 3 cut(s) | 24, 408, 954 |
| FokI | GGATG | 3 cut(s) | 254, 794, 887 |
| FriOI | GRGCYC | 1 cut(s) | 175 |
| Fsp4HI | GCNGC | 3 cut(s) | 24, 408, 954 |
| FspBI | CTAG | 5 cut(s) | 411, 572, 636, 839, 1193 |
| GluI | GCNGC | 3 cut(s) | 24, 408, 954 |
| HgaI | GACGC | 1 cut(s) | 979 |
| Hin1II | CATG | 5 cut(s) | 224, 355, 449, 653, 1063 |
| HincII | GTYRAC | 1 cut(s) | 466 |
| HindII | GTYRAC | 1 cut(s) | 466 |
| HinfI | GANTC | 7 cut(s) | 87, 116, 130, 479, 557, 724, 743 |
| HphI | GGTGA | 3 cut(s) | 208, 400, 727 |
| Hpy166II | GTNNAC | 3 cut(s) | 349, 466, 1115 |
| Hpy188I | TCNGA | 3 cut(s) | 291, 660, 1003 |
| Hpy188III | TCNNGA | 5 cut(s) | 176, 221, 446, 490, 650 |
| Hpy8I | GTNNAC | 3 cut(s) | 349, 466, 1115 |
| HpyAV | CCTTC | 4 cut(s) | 510, 594, 772, 1080 |
| HpyCH4III | ACNGT | 1 cut(s) | 656 |
| HpyCH4V | TGCA | 6 cut(s) | 307, 458, 785, 802, 956, 1127 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 56, 224 |
| HpyF3I | CTNAG | 3 cut(s) | 177, 430, 728 |
| Hsp92II | CATG | 5 cut(s) | 224, 355, 449, 653, 1063 |
| Kzo9I | GATC | 3 cut(s) | 166, 638, 735 |
| LmnI | GCTCC | 2 cut(s) | 178, 523 |
| Lsp1109I | GCAGC | 3 cut(s) | 10, 419, 940 |
| LweI | GCATC | 4 cut(s) | 139, 214, 445, 772 |
| MaeI | CTAG | 5 cut(s) | 411, 572, 636, 839, 1193 |
| MaeIII | GTNAC | 3 cut(s) | 141, 931, 944 |
| MalI | GATC | 3 cut(s) | 168, 640, 737 |
| MboI | GATC | 3 cut(s) | 166, 638, 735 |
| MboII | GAAGA | 7 cut(s) | 211, 221, 409, 634, 818, 821, 1102 |
| MfeI | CAATTG | 1 cut(s) | 597 |
| MflI | RGATCY | 1 cut(s) | 735 |
| MhlI | GDGCHC | 1 cut(s) | 175 |
| MluCI | AATT | 7 cut(s) | 67, 363, 391, 510, 597, 1139, 1167 |
| MlyI | GAGTC | 1 cut(s) | 473 |
| MmeI | TCCRAC | 2 cut(s) | 507, 1198 |
| MnlI | CCTC | 5 cut(s) | 172, 397, 706, 873, 1103 |
| MseI | TTAA | 6 cut(s) | 366, 543, 750, 896, 1025, 1200 |
| MspR9I | CCNGG | 1 cut(s) | 29 |
| MunI | CAATTG | 1 cut(s) | 597 |
| MvaI | CCWGG | 1 cut(s) | 29 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 56, 224 |
| NdeII | GATC | 3 cut(s) | 166, 638, 735 |
| NheI | GCTAGC | 1 cut(s) | 410 |
| NlaIII | CATG | 5 cut(s) | 224, 355, 449, 653, 1063 |
| NlaIV | GGNNCC | 1 cut(s) | 174 |
| NmuCI | GTSAC | 1 cut(s) | 944 |
| PagI | TCATGA | 3 cut(s) | 220, 445, 649 |
| PasI | CCCWGGG | 1 cut(s) | 28 |
| PfeI | GAWTC | 6 cut(s) | 87, 116, 130, 557, 724, 743 |
| PkrI | GCNGC | 3 cut(s) | 25, 409, 955 |
| PleI | GAGTC | 1 cut(s) | 473 |
| PpsI | GAGTC | 1 cut(s) | 473 |
| Psp6I | CCWGG | 1 cut(s) | 27 |
| PspGI | CCWGG | 1 cut(s) | 27 |
| PspN4I | GGNNCC | 1 cut(s) | 174 |
| PsuI | RGATCY | 1 cut(s) | 735 |
| RsaI | GTAC | 4 cut(s) | 248, 279, 296, 1116 |
| RsaNI | GTAC | 4 cut(s) | 247, 278, 295, 1115 |
| SaqAI | TTAA | 6 cut(s) | 366, 543, 750, 896, 1025, 1200 |
| SatI | GCNGC | 3 cut(s) | 24, 408, 954 |
| Sau3AI | GATC | 3 cut(s) | 166, 638, 735 |
| SchI | GAGTC | 1 cut(s) | 473 |
| ScrFI | CCNGG | 1 cut(s) | 29 |
| SduI | GDGCHC | 1 cut(s) | 175 |
| SfaNI | GCATC | 4 cut(s) | 139, 214, 445, 772 |
| Sse9I | AATT | 7 cut(s) | 67, 363, 391, 510, 597, 1139, 1167 |
| SsiI | CCGC | 1 cut(s) | 112 |
| SspI | AATATT | 1 cut(s) | 925 |
| SspMI | CTAG | 5 cut(s) | 411, 572, 636, 839, 1193 |
| StyD4I | CCNGG | 1 cut(s) | 27 |
| StyI | CCWWGG | 2 cut(s) | 384, 913 |
| TaaI | ACNGT | 1 cut(s) | 656 |
| TasI | AATT | 7 cut(s) | 67, 363, 391, 510, 597, 1139, 1167 |
| TatI | WGTACW | 1 cut(s) | 294 |
| TfiI | GAWTC | 6 cut(s) | 87, 116, 130, 557, 724, 743 |
| Tru1I | TTAA | 6 cut(s) | 366, 543, 750, 896, 1025, 1200 |
| Tru9I | TTAA | 6 cut(s) | 366, 543, 750, 896, 1025, 1200 |
| TseFI | GTSAC | 1 cut(s) | 944 |
| TseI | GCWGC | 3 cut(s) | 23, 407, 953 |
| Tsp45I | GTSAC | 1 cut(s) | 944 |
| TspDTI | ATGAA | 3 cut(s) | 270, 513, 1023 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 131 |
| XapI | RAATTY | 2 cut(s) | 67, 391 |
| XspI | CTAG | 5 cut(s) | 411, 572, 636, 839, 1193 |