Required for replication-independent chromatin assembly and for the periodic repression of histone gene transcription during the cell cycle
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 3 cut(s) | 688, 748, 779 |
| AatII | GACGTC | 2 cut(s) | 574, 746 |
| AccB1I | GGYRCC | 1 cut(s) | 529 |
| AccBSI | CCGCTC | 1 cut(s) | 2298 |
| AccI | GTMKAC | 2 cut(s) | 1519, 1900 |
| AccII | CGCG | 2 cut(s) | 825, 2291 |
| AccIII | TCCGGA | 1 cut(s) | 418 |
| AciI | CCGC | 9 cut(s) | 112, 429, 825, 1050, 1499, 1704, 2289, 2291, 2298 |
| AclWI | GGATC | 3 cut(s) | 262, 413, 2039 |
| AcoI | YGGCCR | 2 cut(s) | 1026, 2042 |
| AcsI | RAATTY | 2 cut(s) | 45, 2070 |
| AcuI | CTGAAG | 1 cut(s) | 1985 |
| AcyI | GRCGYC | 4 cut(s) | 571, 683, 743, 774 |
| AdeI | CACNNNGTG | 1 cut(s) | 109 |
| AfaI | GTAC | 4 cut(s) | 520, 598, 1648, 1750 |
| AfeI | AGCGCT | 1 cut(s) | 873 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 93, 138, 139, 299, 1024, 1499, 1514, 1726, 1948 |
| AflIII | ACRYGT | 2 cut(s) | 1895, 1930 |
| AgsI | TTSAA | 6 cut(s) | 563, 1175, 1379, 1445, 2021, 2075 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 708 |
| AjnI | CCWGG | 3 cut(s) | 67, 132, 292 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1944, 1976 |
| AloI | GAACNNNNNNTCC | 6 cut(s) | 113, 145, 280, 312, 444, 476 |
| Alw21I | GWGCWC | 2 cut(s) | 426, 953 |
| AlwI | GGATC | 3 cut(s) | 262, 413, 2039 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 257 |
| Aor13HI | TCCGGA | 1 cut(s) | 418 |
| Aor51HI | AGCGCT | 1 cut(s) | 873 |
| AoxI | GGCC | 5 cut(s) | 1026, 1501, 2042, 2299, 2309 |
| ApeKI | GCWGC | 5 cut(s) | 1052, 1398, 1431, 1464, 1575 |
| ApoI | RAATTY | 2 cut(s) | 45, 2070 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 444, 476, 2039, 2071 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2070 |
| AspLEI | GCGC | 3 cut(s) | 511, 825, 874 |
| AspS9I | GGNCC | 3 cut(s) | 1501, 1964, 2300 |
| AsuC2I | CCSGG | 2 cut(s) | 946, 1994 |
| AsuHPI | GGTGA | 6 cut(s) | 110, 405, 434, 1147, 1930, 2224 |
| AvaII | GGWCC | 1 cut(s) | 1964 |
| BaeGI | GKGCMC | 1 cut(s) | 1613 |
| BalI | TGGCCA | 1 cut(s) | 1028 |
| BanI | GGYRCC | 1 cut(s) | 529 |
| BanII | GRGCYC | 3 cut(s) | 330, 426, 942 |
| BbsI | GAAGAC | 1 cut(s) | 775 |
| Bbv12I | GWGCWC | 2 cut(s) | 426, 953 |
| BbvI | GCAGC | 5 cut(s) | 1064, 1385, 1418, 1451, 1562 |
| BccI | CCATC | 2 cut(s) | 1346, 1501 |
| BceAI | ACGGC | 1 cut(s) | 1717 |
| BciT130I | CCWGG | 3 cut(s) | 69, 134, 294 |
| BclI | TGATCA | 2 cut(s) | 193, 262 |
| BcnI | CCSGG | 2 cut(s) | 946, 1994 |
| BfaI | CTAG | 3 cut(s) | 503, 1568, 1823 |
| BfmI | CTRYAG | 1 cut(s) | 378 |
| BfoI | RGCGCY | 1 cut(s) | 875 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 73 |
| BisI | GCNGC | 8 cut(s) | 429, 1053, 1399, 1432, 1465, 1576, 1705, 2299 |
| BlsI | GCNGC | 8 cut(s) | 430, 1054, 1400, 1433, 1466, 1577, 1706, 2300 |
| Bme1390I | CCNGG | 5 cut(s) | 69, 134, 294, 946, 1994 |
| Bme18I | GGWCC | 1 cut(s) | 1964 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 708 |
| BmgT120I | GGNCC | 3 cut(s) | 1501, 1964, 2300 |
| BmiI | GGNNCC | 2 cut(s) | 304, 531 |
| BmrFI | CCNGG | 5 cut(s) | 69, 134, 294, 946, 1994 |
| BmrI | ACTGGG | 2 cut(s) | 12, 1899 |
| BmsI | GCATC | 5 cut(s) | 257, 641, 1931, 2165, 2273 |
| BmuI | ACTGGG | 2 cut(s) | 12, 1899 |
| BpiI | GAAGAC | 1 cut(s) | 775 |
| BpmI | CTGGAG | 4 cut(s) | 441, 808, 839, 1406 |
| Bpu10I | CCTNAGC | 1 cut(s) | 77 |
| BpuEI | CTTGAG | 2 cut(s) | 1811, 2036 |
| BpuMI | CCSGG | 2 cut(s) | 946, 1994 |
| BsaAI | YACGTR | 1 cut(s) | 1933 |
| BsaHI | GRCGYC | 4 cut(s) | 571, 683, 743, 774 |
| BsaI | GGTCTC | 1 cut(s) | 968 |
| BsaJI | CCNNGG | 5 cut(s) | 132, 133, 1029, 1513, 2289 |
| BsaWI | WCCGGW | 3 cut(s) | 402, 418, 1021 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 93, 138, 139, 299, 1024, 1499, 1514, 1726, 1948 |
| Bse118I | RCCGGY | 1 cut(s) | 576 |
| Bse3DI | GCAATG | 3 cut(s) | 363, 907, 1923 |
| BseAI | TCCGGA | 1 cut(s) | 418 |
| BseBI | CCWGG | 3 cut(s) | 69, 134, 294 |
| BseDI | CCNNGG | 5 cut(s) | 132, 133, 1029, 1513, 2289 |
| BseGI | GGATG | 9 cut(s) | 495, 656, 1005, 1478, 1622, 1712, 1745, 1853, 1922 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 93, 138, 139, 299, 1024, 1499, 1514, 1726, 1948 |
| BseMI | GCAATG | 3 cut(s) | 363, 907, 1923 |
| BseMII | CTCAG | 3 cut(s) | 300, 1137, 1657 |
| BseRI | GAGGAG | 1 cut(s) | 986 |
| BseSI | GKGCMC | 1 cut(s) | 1613 |
| BseXI | GCAGC | 5 cut(s) | 1064, 1385, 1418, 1451, 1562 |
| BsgI | GTGCAG | 4 cut(s) | 1227, 1351, 1417, 1561 |
| Bsh1236I | CGCG | 2 cut(s) | 825, 2291 |
| BshFI | GGCC | 5 cut(s) | 1028, 1503, 2044, 2301, 2311 |
| BshNI | GGYRCC | 1 cut(s) | 529 |
| BsiHKAI | GWGCWC | 2 cut(s) | 426, 953 |
| BslFI | GGGAC | 3 cut(s) | 1060, 1495, 2250 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 93, 138, 139, 299, 1024, 1499, 1514, 1726, 1948 |
| BsmBI | CGTCTC | 5 cut(s) | 674, 734, 765, 1789, 1949 |
| BsmFI | GGGAC | 3 cut(s) | 1060, 1495, 2250 |
| BsnI | GGCC | 5 cut(s) | 1028, 1503, 2044, 2301, 2311 |
| Bso31I | GGTCTC | 1 cut(s) | 968 |
| Bsp1286I | GDGCHC | 5 cut(s) | 330, 426, 942, 953, 1613 |
| Bsp13I | TCCGGA | 1 cut(s) | 418 |
| Bsp143I | GATC | 8 cut(s) | 193, 254, 262, 405, 1264, 1275, 1759, 2031 |
| BspACI | CCGC | 9 cut(s) | 112, 429, 825, 1050, 1499, 1704, 2289, 2291, 2298 |
| BspANI | GGCC | 5 cut(s) | 1028, 1503, 2044, 2301, 2311 |
| BspCNI | CTCAG | 3 cut(s) | 301, 1136, 1656 |
| BspEI | TCCGGA | 1 cut(s) | 418 |
| BspFNI | CGCG | 2 cut(s) | 825, 2291 |
| BspHI | TCATGA | 1 cut(s) | 280 |
| BspLI | GGNNCC | 2 cut(s) | 304, 531 |
| BspMAI | CTGCAG | 1 cut(s) | 382 |
| BspPI | GGATC | 3 cut(s) | 262, 413, 2039 |
| BspQI | GCTCTTC | 1 cut(s) | 2004 |
| BspT107I | GGYRCC | 1 cut(s) | 529 |
| BspTNI | GGTCTC | 1 cut(s) | 968 |
| BsrBI | CCGCTC | 1 cut(s) | 2298 |
| BsrDI | GCAATG | 3 cut(s) | 363, 907, 1923 |
| BsrFI | RCCGGY | 1 cut(s) | 576 |
| BssAI | RCCGGY | 1 cut(s) | 576 |
| BssECI | CCNNGG | 5 cut(s) | 132, 133, 1029, 1513, 2289 |
| BssMI | GATC | 8 cut(s) | 193, 254, 262, 405, 1264, 1275, 1759, 2031 |
| BssNI | GRCGYC | 4 cut(s) | 571, 683, 743, 774 |
| BssT1I | CCWWGG | 2 cut(s) | 1029, 1513 |
| Bst2UI | CCWGG | 3 cut(s) | 69, 134, 294 |
| Bst4CI | ACNGT | 6 cut(s) | 979, 985, 1171, 1191, 1600, 1831 |
| Bst6I | CTCTTC | 6 cut(s) | 876, 1380, 1446, 1692, 2004, 2166 |
| BstACI | GRCGYC | 4 cut(s) | 571, 683, 743, 774 |
| BstBAI | YACGTR | 1 cut(s) | 1933 |
| BstC8I | GCNNGC | 6 cut(s) | 426, 578, 582, 991, 1256, 1501 |
| BstDEI | CTNAG | 7 cut(s) | 77, 228, 309, 875, 1123, 1643, 2313 |
| BstDSI | CCRYGG | 1 cut(s) | 2289 |
| BstEII | GGTNACC | 1 cut(s) | 98 |
| BstF5I | GGATG | 9 cut(s) | 495, 656, 1005, 1478, 1622, 1712, 1745, 1853, 1922 |
| BstFNI | CGCG | 2 cut(s) | 825, 2291 |
| BstH2I | RGCGCY | 1 cut(s) | 875 |
| BstHHI | GCGC | 3 cut(s) | 511, 825, 874 |
| BstKTI | GATC | 8 cut(s) | 196, 257, 265, 408, 1267, 1278, 1762, 2034 |
| BstMBI | GATC | 8 cut(s) | 193, 254, 262, 405, 1264, 1275, 1759, 2031 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 73, 1052, 2283 |
| BstNI | CCWGG | 3 cut(s) | 69, 134, 294 |
| BstNSI | RCATGY | 1 cut(s) | 1899 |
| BstPI | GGTNACC | 1 cut(s) | 98 |
| BstSCI | CCNGG | 5 cut(s) | 67, 132, 292, 944, 1992 |
| BstSFI | CTRYAG | 1 cut(s) | 378 |
| BstSLI | GKGCMC | 1 cut(s) | 1613 |
| BstUI | CGCG | 2 cut(s) | 825, 2291 |
| BstV1I | GCAGC | 5 cut(s) | 1064, 1385, 1418, 1451, 1562 |
| BstV2I | GAAGAC | 1 cut(s) | 775 |
| BstX2I | RGATCY | 2 cut(s) | 254, 2031 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1036 |
| BstYI | RGATCY | 2 cut(s) | 254, 2031 |
| BsuRI | GGCC | 5 cut(s) | 1028, 1503, 2044, 2301, 2311 |
| BtgI | CCRYGG | 1 cut(s) | 2289 |
| BtsCI | GGATG | 9 cut(s) | 495, 656, 1005, 1478, 1622, 1712, 1745, 1853, 1922 |
| BtsI | GCAGTG | 2 cut(s) | 325, 1541 |
| BtsIMutI | CAGTG | 6 cut(s) | 87, 306, 325, 477, 1541, 1912 |
| Cac8I | GCNNGC | 6 cut(s) | 426, 578, 582, 991, 1256, 1501 |
| CaiI | CAGNNNCTG | 1 cut(s) | 257 |
| CciI | TCATGA | 1 cut(s) | 280 |
| CfoI | GCGC | 3 cut(s) | 511, 825, 874 |
| Cfr10I | RCCGGY | 1 cut(s) | 576 |
| Cfr13I | GGNCC | 3 cut(s) | 1501, 1964, 2300 |
| Cfr42I | CCGCGG | 1 cut(s) | 2292 |
| CseI | GACGC | 2 cut(s) | 691, 782 |
| Csp6I | GTAC | 4 cut(s) | 519, 597, 1647, 1749 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 295, 330 |
| CviAII | CATG | 7 cut(s) | 32, 281, 662, 1036, 1231, 1270, 1896 |
| CviQI | GTAC | 4 cut(s) | 519, 597, 1647, 1749 |
| DdeI | CTNAG | 7 cut(s) | 77, 228, 309, 875, 1123, 1643, 2313 |
| DpnI | GATC | 8 cut(s) | 195, 256, 264, 407, 1266, 1277, 1761, 2033 |
| DpnII | GATC | 8 cut(s) | 193, 254, 262, 405, 1264, 1275, 1759, 2031 |
| DraIII | CACNNNGTG | 1 cut(s) | 109 |
| DrdI | GACNNNNNNGTC | 3 cut(s) | 688, 748, 779 |
| DriI | GACNNNNNGTC | 1 cut(s) | 708 |
| DseDI | GACNNNNNNGTC | 3 cut(s) | 688, 748, 779 |
| EaeI | YGGCCR | 2 cut(s) | 1026, 2042 |
| Eam1104I | CTCTTC | 6 cut(s) | 876, 1380, 1446, 1692, 2004, 2166 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 708 |
| EarI | CTCTTC | 6 cut(s) | 876, 1380, 1446, 1692, 2004, 2166 |
| Ecl136II | GAGCTC | 1 cut(s) | 424 |
| Eco130I | CCWWGG | 2 cut(s) | 1029, 1513 |
| Eco24I | GRGCYC | 3 cut(s) | 330, 426, 942 |
| Eco31I | GGTCTC | 1 cut(s) | 968 |
| Eco47I | GGWCC | 1 cut(s) | 1964 |
| Eco47III | AGCGCT | 1 cut(s) | 873 |
| Eco53kI | GAGCTC | 1 cut(s) | 424 |
| Eco57I | CTGAAG | 1 cut(s) | 1985 |
| Eco91I | GGTNACC | 1 cut(s) | 98 |
| EcoICRI | GAGCTC | 1 cut(s) | 424 |
| EcoO65I | GGTNACC | 1 cut(s) | 98 |
| EcoRII | CCWGG | 3 cut(s) | 67, 132, 292 |
| EcoT14I | CCWWGG | 2 cut(s) | 1029, 1513 |
| EcoT38I | GRGCYC | 3 cut(s) | 330, 426, 942 |
| ErhI | CCWWGG | 2 cut(s) | 1029, 1513 |
| Esp3I | CGTCTC | 5 cut(s) | 674, 734, 765, 1789, 1949 |
| FaeI | CATG | 7 cut(s) | 35, 284, 665, 1039, 1234, 1273, 1899 |
| FaqI | GGGAC | 3 cut(s) | 1060, 1495, 2250 |
| FatI | CATG | 7 cut(s) | 31, 280, 661, 1035, 1230, 1269, 1895 |
| FauI | CCCGC | 3 cut(s) | 105, 818, 1492 |
| FbaI | TGATCA | 2 cut(s) | 193, 262 |
| FblI | GTMKAC | 2 cut(s) | 1519, 1900 |
| Fnu4HI | GCNGC | 8 cut(s) | 429, 1053, 1399, 1432, 1465, 1576, 1705, 2299 |
| FokI | GGATG | 9 cut(s) | 482, 663, 992, 1465, 1609, 1699, 1752, 1840, 1909 |
| FriOI | GRGCYC | 3 cut(s) | 330, 426, 942 |
| Fsp4HI | GCNGC | 8 cut(s) | 429, 1053, 1399, 1432, 1465, 1576, 1705, 2299 |
| FspBI | CTAG | 3 cut(s) | 503, 1568, 1823 |
| GlaI | GCGC | 3 cut(s) | 510, 824, 873 |
| GluI | GCNGC | 8 cut(s) | 429, 1053, 1399, 1432, 1465, 1576, 1705, 2299 |
| GsuI | CTGGAG | 4 cut(s) | 441, 808, 839, 1406 |
| HaeII | RGCGCY | 1 cut(s) | 875 |
| HaeIII | GGCC | 5 cut(s) | 1028, 1503, 2044, 2301, 2311 |
| HgaI | GACGC | 2 cut(s) | 691, 782 |
| HhaI | GCGC | 3 cut(s) | 511, 825, 874 |
| Hin1I | GRCGYC | 4 cut(s) | 571, 683, 743, 774 |
| Hin1II | CATG | 7 cut(s) | 35, 284, 665, 1039, 1234, 1273, 1899 |
| Hin6I | GCGC | 3 cut(s) | 509, 823, 872 |
| HinP1I | GCGC | 3 cut(s) | 509, 823, 872 |
| HincII | GTYRAC | 2 cut(s) | 1085, 1520 |
| HindII | GTYRAC | 2 cut(s) | 1085, 1520 |
| HphI | GGTGA | 6 cut(s) | 110, 405, 434, 1147, 1930, 2224 |
| Hpy166II | GTNNAC | 6 cut(s) | 1085, 1346, 1520, 1565, 1751, 1901 |
| Hpy188I | TCNGA | 8 cut(s) | 259, 391, 470, 557, 748, 1126, 1152, 2196 |
| Hpy8I | GTNNAC | 6 cut(s) | 1085, 1346, 1520, 1565, 1751, 1901 |
| Hpy99I | CGWCG | 6 cut(s) | 470, 573, 576, 748, 1083, 1725 |
| HpyAV | CCTTC | 4 cut(s) | 921, 1028, 1664, 2217 |
| HpyCH4III | ACNGT | 6 cut(s) | 979, 985, 1171, 1191, 1600, 1831 |
| HpyCH4IV | ACGT | 7 cut(s) | 123, 571, 586, 743, 1128, 1412, 1932 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 73, 1052, 2283 |
| HpyF3I | CTNAG | 7 cut(s) | 77, 228, 309, 875, 1123, 1643, 2313 |
| HpySE526I | ACGT | 7 cut(s) | 123, 571, 586, 743, 1128, 1412, 1932 |
| Hsp92I | GRCGYC | 4 cut(s) | 571, 683, 743, 774 |
| Hsp92II | CATG | 7 cut(s) | 35, 284, 665, 1039, 1234, 1273, 1899 |
| HspAI | GCGC | 3 cut(s) | 509, 823, 872 |
| Kpn2I | TCCGGA | 1 cut(s) | 418 |
| KroI | GCCGGC | 1 cut(s) | 576 |
| KroNI | GCCGGC | 1 cut(s) | 578 |
| Ksp22I | TGATCA | 2 cut(s) | 193, 262 |
| KspI | CCGCGG | 1 cut(s) | 2292 |
| Kzo9I | GATC | 8 cut(s) | 193, 254, 262, 405, 1264, 1275, 1759, 2031 |
| LguI | GCTCTTC | 1 cut(s) | 2004 |
| LmnI | GCTCC | 9 cut(s) | 421, 529, 667, 789, 820, 910, 948, 1395, 1461 |
| Lsp1109I | GCAGC | 5 cut(s) | 1064, 1385, 1418, 1451, 1562 |
| LweI | GCATC | 5 cut(s) | 257, 641, 1931, 2165, 2273 |
| MaeI | CTAG | 3 cut(s) | 503, 1568, 1823 |
| MaeII | ACGT | 7 cut(s) | 123, 571, 586, 743, 1128, 1412, 1932 |
| MaeIII | GTNAC | 4 cut(s) | 98, 979, 1279, 1618 |
| MalI | GATC | 8 cut(s) | 195, 256, 264, 407, 1266, 1277, 1761, 2033 |
| MbiI | CCGCTC | 1 cut(s) | 2298 |
| MboI | GATC | 8 cut(s) | 193, 254, 262, 405, 1264, 1275, 1759, 2031 |
| MfeI | CAATTG | 2 cut(s) | 1632, 2260 |
| MflI | RGATCY | 2 cut(s) | 254, 2031 |
| MhlI | GDGCHC | 5 cut(s) | 330, 426, 942, 953, 1613 |
| MlsI | TGGCCA | 1 cut(s) | 1028 |
| MluNI | TGGCCA | 1 cut(s) | 1028 |
| MlyI | GAGTC | 7 cut(s) | 667, 698, 718, 727, 758, 820, 1691 |
| MmeI | TCCRAC | 3 cut(s) | 448, 580, 726 |
| Mox20I | TGGCCA | 1 cut(s) | 1028 |
| MroI | TCCGGA | 1 cut(s) | 418 |
| MroNI | GCCGGC | 1 cut(s) | 576 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2070 |
| MscI | TGGCCA | 1 cut(s) | 1028 |
| MseI | TTAA | 1 cut(s) | 1892 |
| Msp20I | TGGCCA | 1 cut(s) | 1028 |
| MspA1I | CMGCKG | 1 cut(s) | 2291 |
| MspR9I | CCNGG | 5 cut(s) | 69, 134, 294, 946, 1994 |
| MunI | CAATTG | 2 cut(s) | 1632, 2260 |
| MvaI | CCWGG | 3 cut(s) | 69, 134, 294 |
| MvnI | CGCG | 2 cut(s) | 825, 2291 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 73, 1052, 2283 |
| NaeI | GCCGGC | 1 cut(s) | 578 |
| NciI | CCSGG | 2 cut(s) | 946, 1994 |
| NdeII | GATC | 8 cut(s) | 193, 254, 262, 405, 1264, 1275, 1759, 2031 |
| NgoMIV | GCCGGC | 1 cut(s) | 576 |
| NlaIII | CATG | 7 cut(s) | 35, 284, 665, 1039, 1234, 1273, 1899 |
| NlaIV | GGNNCC | 2 cut(s) | 304, 531 |
| NmuCI | GTSAC | 2 cut(s) | 98, 979 |
| NspI | RCATGY | 1 cut(s) | 1899 |
| PagI | TCATGA | 1 cut(s) | 280 |
| PasI | CCCWGGG | 1 cut(s) | 133 |
| PciI | ACATGT | 1 cut(s) | 1895 |
| PciSI | GCTCTTC | 1 cut(s) | 2004 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 689, 718, 749 |
| PdiI | GCCGGC | 1 cut(s) | 578 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2070 |
| PfeI | GAWTC | 8 cut(s) | 158, 410, 514, 1731, 1985, 2021, 2183, 2191 |
| PflFI | GACNNNGTC | 1 cut(s) | 1072 |
| PfoI | TCCNGGA | 1 cut(s) | 944 |
| PkrI | GCNGC | 8 cut(s) | 430, 1054, 1400, 1433, 1466, 1577, 1706, 2300 |
| PleI | GAGTC | 7 cut(s) | 666, 697, 717, 726, 757, 819, 1690 |
| PpsI | GAGTC | 7 cut(s) | 666, 697, 717, 726, 757, 819, 1690 |
| Ppu21I | YACGTR | 1 cut(s) | 1933 |
| PscI | ACATGT | 1 cut(s) | 1895 |
| Psp124BI | GAGCTC | 1 cut(s) | 426 |
| Psp6I | CCWGG | 3 cut(s) | 67, 132, 292 |
| PspEI | GGTNACC | 1 cut(s) | 98 |
| PspGI | CCWGG | 3 cut(s) | 67, 132, 292 |
| PspN4I | GGNNCC | 2 cut(s) | 304, 531 |
| PspPI | GGNCC | 3 cut(s) | 1501, 1964, 2300 |
| PstI | CTGCAG | 1 cut(s) | 382 |
| PstNI | CAGNNNCTG | 1 cut(s) | 257 |
| PsuI | RGATCY | 2 cut(s) | 254, 2031 |
| PsyI | GACNNNGTC | 1 cut(s) | 1072 |
| RsaI | GTAC | 4 cut(s) | 520, 598, 1648, 1750 |
| RsaNI | GTAC | 4 cut(s) | 519, 597, 1647, 1749 |
| SacI | GAGCTC | 1 cut(s) | 426 |
| SacII | CCGCGG | 1 cut(s) | 2292 |
| SalI | GTCGAC | 1 cut(s) | 1518 |
| SapI | GCTCTTC | 1 cut(s) | 2004 |
| SaqAI | TTAA | 1 cut(s) | 1892 |
| SatI | GCNGC | 8 cut(s) | 429, 1053, 1399, 1432, 1465, 1576, 1705, 2299 |
| Sau3AI | GATC | 8 cut(s) | 193, 254, 262, 405, 1264, 1275, 1759, 2031 |
| Sau96I | GGNCC | 3 cut(s) | 1501, 1964, 2300 |
| SchI | GAGTC | 7 cut(s) | 667, 698, 718, 727, 758, 820, 1691 |
| ScrFI | CCNGG | 5 cut(s) | 69, 134, 294, 946, 1994 |
| SduI | GDGCHC | 5 cut(s) | 330, 426, 942, 953, 1613 |
| SfaNI | GCATC | 5 cut(s) | 257, 641, 1931, 2165, 2273 |
| SfcI | CTRYAG | 1 cut(s) | 378 |
| Sfr303I | CCGCGG | 1 cut(s) | 2292 |
| SgrBI | CCGCGG | 1 cut(s) | 2292 |
| SinI | GGWCC | 1 cut(s) | 1964 |
| SmlI | CTYRAG | 2 cut(s) | 1790, 2051 |
| SmoI | CTYRAG | 2 cut(s) | 1790, 2051 |
| SsiI | CCGC | 9 cut(s) | 112, 429, 825, 1050, 1499, 1704, 2289, 2291, 2298 |
| SspMI | CTAG | 3 cut(s) | 503, 1568, 1823 |
| SstI | GAGCTC | 1 cut(s) | 426 |
| StyD4I | CCNGG | 5 cut(s) | 67, 132, 292, 944, 1992 |
| StyI | CCWWGG | 2 cut(s) | 1029, 1513 |
| TaaI | ACNGT | 6 cut(s) | 979, 985, 1171, 1191, 1600, 1831 |
| TaiI | ACGT | 7 cut(s) | 126, 574, 589, 746, 1131, 1415, 1935 |
| TaqI | TCGA | 9 cut(s) | 408, 546, 568, 629, 1291, 1519, 1657, 1723, 2181 |
| TatI | WGTACW | 2 cut(s) | 1646, 1748 |
| TauI | GCSGC | 3 cut(s) | 431, 1707, 2301 |
| TfiI | GAWTC | 8 cut(s) | 158, 410, 514, 1731, 1985, 2021, 2183, 2191 |
| Tru1I | TTAA | 1 cut(s) | 1892 |
| Tru9I | TTAA | 1 cut(s) | 1892 |
| TscAI | CASTG | 6 cut(s) | 94, 313, 325, 484, 1541, 1912 |
| TseFI | GTSAC | 2 cut(s) | 98, 979 |
| TseI | GCWGC | 5 cut(s) | 1052, 1398, 1431, 1464, 1575 |
| Tsp45I | GTSAC | 2 cut(s) | 98, 979 |
| TspDTI | ATGAA | 4 cut(s) | 168, 269, 1131, 1977 |
| TspGWI | ACGGA | 8 cut(s) | 179, 1408, 1709, 1752, 1850, 1956, 1964, 2200 |
| TspRI | CASTG | 6 cut(s) | 94, 313, 325, 484, 1541, 1912 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1072 |
| VpaK11BI | GGWCC | 1 cut(s) | 1964 |
| XapI | RAATTY | 2 cut(s) | 45, 2070 |
| XbaI | TCTAGA | 1 cut(s) | 1822 |
| XceI | RCATGY | 1 cut(s) | 1899 |
| XmiI | GTMKAC | 2 cut(s) | 1519, 1900 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2070 |
| XspI | CTAG | 3 cut(s) | 503, 1568, 1823 |
| ZraI | GACGTC | 2 cut(s) | 572, 744 |