RchiOBHm_Chr4g0418861

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000029
Physical Location & Seq
Forward (+)
44315813 .. 44320580
4768 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38880

Sequence Viewer

Length: 3708 bp
ATGCATGTACTCTCTGTCCTTACTCCCGTTGCCGGAGGAGTAGTGAGTAAGGTGGCTTTACTTGCTACTGATCAGTTGAGACTCGATATCATCTGGGGGTTCCGCGAAGAGATAAATAAGCTCAAAGAATCCTCAATTCTGATTGGAAATATAATCCATGATGCTGCTCACGAACCTGAAGATGAGGATCTGTGGGCAAGAGCCGAGTGGATGAAGAGGTTGATAAGCGTAGCCTATGATGCAGAAGATGTCCTGGATAAATTTCAATATGAAGTTTATCGGATCGTTATAAAGGAGACAAGCCTCGATAAGAAGATACTCGGCTTCTTCTGCAGCAATCCTCTTGTATTTCGTCTCAAGATGGCCCGCAATATTTACAATATCAACACGTCCTTGGAGGATCTCTACAAGCAAGCAGCTGGCGTGGGAGTCGTCAGATCAAATGGAGGTGCAGCAGCCAGTCAAGGTATGCAGGACAGGGAAACAACCTCATTCTTTGAGAAAAATGAATTCACCTTCAAAGATGCAATCACTGTTGGAAGGGATCAGGTTGTGTCAGATATAATTGCAACCTTGACCAACTCCAACAATCAAGAAAATATTCTTTCGGTGATGGGCATTGTGGGATTGGGAGGCCTAGGTAAAACAACTTTGGCTCGCTTAATTACCAAGCAACTGAAACAAGGTGAAATGGAAAAGCACTTTGATACAACGCTCTGGGTGTACGTATCTAATACTTTTCATGTCAATTCAATTTTACATCGAATGGTGCAATCATGTGGCGTGACAGGAATTGACCCTTCAAATCAGCAAGCATTGATGGAAAGCCTCCAGCAGAAGTTGGAACAGAAAAGGTATTTTCTTGTACTTGATGACGTCTGGAATGAGGATGCTAATAAGTGGAATGATTTGAAGAGTTGTTTATTGCAACTAAATTCTGCCAGAGGAAGCTCTGTGATTGTCACCGCTCGTAGATCCAATGTTGCATCAATCATGGAAACACTTCCTAGGTGGAATTTAGAAACTTTATCGGATGATGAATGTTGGTCCATATTGAAGGATAGAGCATTTGTAGATCCTAATGCTCCCATAGCTTCAGAACTAGAGGAAATTGGAAGGGACATTGCCAAAAAGTGTGCTGGTCTTCCATTGGCAGCCAAGGTTTTGGGAAGCTTGATGCGTTCTAAGACCAGTATGAAGGAATGGTTGTCAATTCTAGAAAGTCCAATATGGCAGTTATCATCCGACAATGAGGATAGTAGGATCATGTCGGTTTTGAAGTTGAGTTATGACAACTTGAAATCACCACTGAAGCAATGTTTTGCATATTGCTCCATCTTCGAGAGAGGTTCCTCAATTGAAAGTGATAGCTTGATCCAACTATGGATGGCTCAAGGTTTTCTCCATCCTTCTTATGGTCACCAAACCAAGCAAGAGATTGAAATGGAGGATATAGGCAACAAGTATTTTGAGACTCTACTGGAGAACTCCTTGTTTCAAGATGCTACAGAGTATGAGGATGTCTGTGGTGTTATCATCACCCGATGCAAGATGCATGATCTTGTGCATGATCTTGCAAATGAAGTATCCAAGTGTGAGAGCTTGATGCCAGACTTCACTCATGAGAAGGATCATCGTGATATACTTATTCGACATGTTGCACGGATTCCTACTCCAACGCTAGAAGAAATGTCAGAAAGAAGTATTTTAAGACTGCGATCACTCTTTTCAAATGGCCCTGTCTCTAATACCATTTTCCAAAAGCTTACAACTTTACATGTCTTAAGTTTCTCTAGTGATGAGATTATGAAGTTGCCAAATTTACTCGGCAAGCTGAAGCACTTGAGGTATCTAGATCTTTCCTGTACAGACATTGAAGCACTCCCAAAGTCTATTGGCAAGCTCTATAACCTCCAAACATTGAGATTACCGCGGTATCTCATAGAGTGTCCGAAGGAGGTTCAAAATTTGATCAATTTAAGGCATCTTTATTGTGGTCTTGAGGGAATGAAATTTAAGGCTGGGGTTTTGGAGCGGTTAACTAATCTCCGAAGATTGTCTTCCTTTAATGTGGGTGAGGAGAGAGGTCCTGCAATTAAGGAGTTGGGTGGGTTGAACCAATTGAGAGGCCACTTACTTATTGATGGACTAGAACATGTAAGGGATGGAGAAGAAGCAAAGAAGGCTCGCTTAGTGCGGAAGAGTCACTTACGCAAGTTGACATTTGGATGGACACACTATTGGCTACGTAATGAAAAGCAGACGGATGTACTAGATGGCTTGGAACCTCATGGTAACTTGCAAAGCTTGGAGATTTACCATTTCAAGGGTGCTAGATTTCCGTCATGGATAACGAGTCTCCAAAATTTGAAGCAGATTCGATTAGTCGAATGCAACAATTGTGAAGGAGTCCCAACACTCGGCCATCTACCTAATCTAACATCTCTTGCGATTAAAAGGATGGAAAAGCTGAAACGTGTGGGAGCTGAGTTTTATGGTTATAGTGATACTAGCACAACTTTATTTCCAGCTCTGAAAACACTATCCATCTCTGAGTGCCGAGAGCTCATTGAATGGACGGAAGCACCGAGGATATTAGCTGAAAGAGAGGTCGTCGTCGTATTTCCTTGCCTCGAAGAGTTGAGGCTATCTGGTTGTGACAGCCTAGAGTCGATTAAGATCACACAGGGCATCGCAGCTCTCCGTCAATTGAAGATCGATGATTGTATGGGATTATCAAGCCTAGAGGTTGGGCTCGATTACTGCACCTCTCTTCAGCAATTGAACATCTTCAATTGCGGTAATCTGATGACAATTCCAACCGCACAAGGCATGCCGTCTCTCCGGAAATTGAATATCGAAAGTTGTGAGGGATTGTCAAGCCTAGGAAGTGGGCTCAACTACTGCACCTCTCTTCAGGAATTGAAGCTATTGAGATGTGATAATCTAACATCAATTCCAATCACACAGGGCATCACATCTCTCCGGAGCTTGACGATTGTTCGTTGTCAGAGATTATTAAGCCTACCGAGTGGGCTCCAATTCTGCACCTCTCTTGAGGATCTGATGATAACTCGTTGCTCTAGTCTAGTATCATATGTGTGCGACCCCCCGGACTCAACGAATCAACCGAAGGAAGTCTCTACAGGAGTTGCTGAGTATTTGTCTGTACAGGAGGAGGATTGCCCTAGTCTAGAATCTATTCCGCCTAATCTCCATCAGTTGTTGATATGTCATTGTGGGAAATTCAAATATGTACCCACAGGAGGATTTCAACGCCTCACTCGTTTGAAGAGGCTCACAATTGGTCCCTTCTGGGAGGAGCTGGACGCCTTCCCTGATTTTCAGCATCCACCACCACATGATTCACAACTTGAAGACTTAGAGTTGCACGGGTGGCCTAAGGTCAAGTCTCTGCCCCAACAAATTCAGCACTTGACTTGTTTAAAAGAACTCTGGATAAGTGATTTCAAGGGACTAGAAGCTCTTCCTGAGTGGTTGGGAAATCTTACATCTCTTGAGGCTCTGAAAATCGGGCTTTGTGAGAATCTCAAATATCTACCTACACAAGATGCTATGAAAAACCTCACCGAATTGAAGTCGCTCTTCATTAGGGGATGTCCCCTGCTCAAAGAAAGATGCACCAAGGAGACTGGCCCAGAATGGCCCAAGATTTCTCACATCCCAGATCTTCGGTTTTGCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 ko04014 map03460 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1235

Amino Acids

139.63

Weight (kDa)

5.95

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 14 - 93 2.5e-10 Rx N-terminal domain
NB-ARC PF00931 182 - 357 1.8e-33 NB-ARC domain
AAA_22 PF13401 206 - 316 8.8e-08 AAA domain
WHD_DRP PF23559 446 - 526 2.8e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 572 - 851 3.9e-32 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 699 - 821 3.5e-37 R13L1/DRL21 LRRs
LRR_14 PF23598 1125 - 1216 3.2e-07 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 1142 - 1206 5.7e-06 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000029)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g13530 FvH4_4g18270 FvH4_6g12200 FvH4_6g12460 FvH4_6g12461 FvH4_6g13450 FvH4_6g15090 FvH4_6g15220 FvH4_6g15230 FvH4_6g15250 FvH4_6g15251 FvH4_6g15340 FvH4_6g47050 FvH4_6g47050 FvH4_6g47050 FvH4_6g47050 FvH4_6g47050 FvH4_6g47980 FvH4_6g47980 FvH4_6g47980 FvH4_6g48170 FvH4_6g48310 FvH4_6g48310 FvH4_6g48850 FvH4_6g48881 FvH4_7g11830
malus_domestica MD02G1059900.v1.1 MD02G1069600.v1.1 MD02G1069700.v1.1 MD02G1070100.v1.1 MD02G1070200.v1.1 MD02G1070300.v1.1 MD02G1070800.v1.1 MD02G1070900.v1.1 MD02G1071200.v1.1 MD02G1071400.v1.1 MD02G1071900.v1.1 MD02G1075200.v1.1 MD03G1048000.v1.1 MD03G1049100.v1.1 MD03G1049200.v1.1 MD03G1052500.v1.1 MD03G1054100.v1.1 MD03G1054300.v1.1 MD03G1054400.v1.1 MD04G1113400.v1.1 MD04G1211100.v1.1 MD04G1211200.v1.1 MD05G1174900.v1.1 MD05G1175000.v1.1 MD05G1175900.v1.1 MD05G1243400.v1.1 MD07G1031500.v1.1 MD07G1031600.v1.1 MD07G1032500.v1.1 MD11G1046800.v1.1 MD11G1046900.v1.1 MD11G1047000.v1.1 MD11G1050400.v1.1 MD11G1050600.v1.1 MD11G1050800.v1.1 MD11G1051100.v1.1 MD11G1051600.v1.1 MD11G1051700.v1.1 MD11G1054600.v1.1 MD11G1055600.v1.1 MD11G1055700.v1.1 MD11G1055800.v1.1 MD11G1056000.v1.1 MD12G1126600.v1.1 MD12G1126700.v1.1 MD12G1126800.v1.1 MD12G1127300.v1.1 MD12G1127600.v1.1 MD12G1127900.v1.1 MD12G1128100.v1.1 MD12G1128300.v1.1 MD15G1261500.v1.1
prunus_persica Prupe.1G010700_v2.0.a1 Prupe.1G010800_v2.0.a1 Prupe.1G011000_v2.0.a1 Prupe.1G159600_v2.0.a1 Prupe.1G163600_v2.0.a1 Prupe.1G163600_v2.0.a1 Prupe.1G163600_v2.0.a1 Prupe.1G163600_v2.0.a1 Prupe.1G163700_v2.0.a1 Prupe.1G163700_v2.0.a1 Prupe.1G163800_v2.0.a1 Prupe.1G164000_v2.0.a1 Prupe.1G164000_v2.0.a1 Prupe.1G164000_v2.0.a1 Prupe.1G164000_v2.0.a1 Prupe.1G235500_v2.0.a1 Prupe.2G067400_v2.0.a1 Prupe.2G067500_v2.0.a1 Prupe.2G111700_v2.0.a1 Prupe.2G111800_v2.0.a1 Prupe.3G012000_v2.0.a1 Prupe.6G243400_v2.0.a1 Prupe.6G243500_v2.0.a1 Prupe.6G243600_v2.0.a1 Prupe.6G243700_v2.0.a1 Prupe.6G243800_v2.0.a1 Prupe.6G243900_v2.0.a1
pyrus_communis pycom02g04860 pycom02g04880 pycom02g04890 pycom02g05510 pycom02g05520 pycom02g05600 pycom02g05930 pycom02g06000 pycom02g06010 pycom03g03800 pycom03g03860 pycom03g03880 pycom03g03890 pycom03g03910 pycom03g03920 pycom03g04200 pycom03g04290 pycom03g04300 pycom03g04310 pycom03g04320 pycom03g09260 pycom04g10150 pycom04g10160 pycom04g10170 pycom05g16180 pycom05g16190 pycom05g16240 pycom05g21980 pycom05g21990 pycom07g02280 pycom11g03800 pycom11g03810 pycom11g04080 pycom11g04140 pycom11g04150 pycom11g04160 pycom11g04570 pycom11g04580 pycom11g04590 pycom11g04610 pycom12g12470 pycom12g12490 pycom13g22050
rosa_chinensis RchiOBHm_Chr1g0317761 RchiOBHm_Chr1g0317821 RchiOBHm_Chr1g0317831 RchiOBHm_Chr1g0317871 RchiOBHm_Chr1g0350501 RchiOBHm_Chr2g0107631 RchiOBHm_Chr3g0452091 RchiOBHm_Chr3g0464001 RchiOBHm_Chr3g0464281 RchiOBHm_Chr3g0466331 RchiOBHm_Chr3g0467341 RchiOBHm_Chr3g0467381 RchiOBHm_Chr3g0467731 RchiOBHm_Chr3g0468291 RchiOBHm_Chr3g0468321 RchiOBHm_Chr3g0468341 RchiOBHm_Chr3g0468391 RchiOBHm_Chr3g0468411 RchiOBHm_Chr3g0468571 RchiOBHm_Chr3g0468591 RchiOBHm_Chr3g0490721 RchiOBHm_Chr3g0490751 RchiOBHm_Chr3g0490761 RchiOBHm_Chr3g0490771 RchiOBHm_Chr3g0490951 RchiOBHm_Chr3g0491001 RchiOBHm_Chr3g0491011 RchiOBHm_Chr4g0412461 RchiOBHm_Chr4g0414161 RchiOBHm_Chr4g0414181 RchiOBHm_Chr4g0414191 RchiOBHm_Chr4g0414281 RchiOBHm_Chr4g0414441 RchiOBHm_Chr4g0414521 RchiOBHm_Chr4g0414531 RchiOBHm_Chr4g0417011 RchiOBHm_Chr4g0417021 RchiOBHm_Chr4g0417041 RchiOBHm_Chr4g0417071 RchiOBHm_Chr4g0417101 RchiOBHm_Chr4g0417531 RchiOBHm_Chr4g0417641 RchiOBHm_Chr4g0417651 RchiOBHm_Chr4g0417701 RchiOBHm_Chr4g0417731 RchiOBHm_Chr4g0417741 RchiOBHm_Chr4g0417781 RchiOBHm_Chr4g0418751 RchiOBHm_Chr4g0418791 RchiOBHm_Chr4g0418861 RchiOBHm_Chr4g0418881 RchiOBHm_Chr4g0418901 RchiOBHm_Chr4g0422451 RchiOBHm_Chr4g0422461 RchiOBHm_Chr4g0422471 RchiOBHm_Chr4g0422541 RchiOBHm_Chr4g0422561 RchiOBHm_Chr4g0422591 RchiOBHm_Chr4g0422651 RchiOBHm_Chr4g0422661 RchiOBHm_Chr5g0003251 RchiOBHm_Chr5g0003261 RchiOBHm_Chr5g0055871 RchiOBHm_Chr7g0204701 RchiOBHm_Chr7g0204741
rosa_laevigata RLG00000007607 RLG00000007932 RLG00000017606 RLG00000024418 RLG00000025629 RLG00000030586
rosa_multiflora Rmu_co7959167.1_g000001 Rmu_co8021330.1_g000001 Rmu_co8132128.1_g000001 Rmu_co8224744.1_g000001 Rmu_co8281865.1_g000001 Rmu_co8298487.1_g000001 Rmu_co8319035.1_g000001 Rmu_co8380035.1_g000001 Rmu_co8385371.1_g000001 Rmu_co8398173.1_g000001 Rmu_co8423363.1_g000001 Rmu_co8438275.1_g000001 Rmu_co8474729.1_g000001 Rmu_sc0000057.1_g000008 Rmu_sc0000350.1_g000023 Rmu_sc0000480.1_g000005 Rmu_sc0000654.1_g000021 Rmu_sc0000654.1_g000029 Rmu_sc0000654.1_g000032 Rmu_sc0000654.1_g000041 Rmu_sc0000654.1_g000045 Rmu_sc0000772.1_g000002 Rmu_sc0000772.1_g000041 Rmu_sc0000808.1_g000040 Rmu_sc0000830.1_g000062 Rmu_sc0000830.1_g000065 Rmu_sc0000830.1_g000072 Rmu_sc0000830.1_g000073 Rmu_sc0000935.1_g000020 Rmu_sc0000935.1_g000024 Rmu_sc0000935.1_g000026 Rmu_sc0000935.1_g000027 Rmu_sc0000935.1_g000031 Rmu_sc0000935.1_g000033 Rmu_sc0000962.1_g000033 Rmu_sc0000962.1_g000036 Rmu_sc0000962.1_g000063 Rmu_sc0000962.1_g000064 Rmu_sc0000962.1_g000065 Rmu_sc0001029.1_g000007 Rmu_sc0001063.1_g000014 Rmu_sc0001149.1_g000010 Rmu_sc0001149.1_g000011 Rmu_sc0001358.1_g000009 Rmu_sc0001448.1_g000003 Rmu_sc0001448.1_g000004 Rmu_sc0001729.1_g000005 Rmu_sc0001729.1_g000007 Rmu_sc0001729.1_g000020 Rmu_sc0001729.1_g000023 Rmu_sc0001793.1_g000015 Rmu_sc0002494.1_g000018 Rmu_sc0002494.1_g000019 Rmu_sc0002604.1_g000022 Rmu_sc0002922.1_g000011 Rmu_sc0003008.1_g000009 Rmu_sc0003221.1_g000061 Rmu_sc0003221.1_g000062 Rmu_sc0003459.1_g000014 Rmu_sc0003480.1_g000015 Rmu_sc0003541.1_g000016 Rmu_sc0003541.1_g000020 Rmu_sc0004035.1_g000001 Rmu_sc0004035.1_g000005 Rmu_sc0004035.1_g000011 Rmu_sc0004142.1_g000004 Rmu_sc0004142.1_g000010 Rmu_sc0004353.1_g000008 Rmu_sc0004616.1_g000009 Rmu_sc0004616.1_g000010 Rmu_sc0005231.1_g000004 Rmu_sc0006380.1_g000025 Rmu_sc0006492.1_g000010 Rmu_sc0006895.1_g000001 Rmu_sc0006895.1_g000002 Rmu_sc0006895.1_g000005 Rmu_sc0007034.1_g000004 Rmu_sc0007104.1_g000006 Rmu_sc0008990.1_g000003 Rmu_sc0008990.1_g000004 Rmu_sc0009106.1_g000003 Rmu_sc0009516.1_g000002 Rmu_sc0009516.1_g000003 Rmu_sc0009516.1_g000004 Rmu_sc0009516.1_g000005 Rmu_sc0009645.1_g000001 Rmu_sc0010274.1_g000001 Rmu_sc0010274.1_g000002 Rmu_sc0010419.1_g000003 Rmu_sc0010917.1_g000017 Rmu_sc0011213.1_g000001 Rmu_sc0011228.1_g000007 Rmu_sc0011538.1_g000011 Rmu_sc0011538.1_g000014 Rmu_sc0011538.1_g000015 Rmu_sc0012211.1_g000006 Rmu_sc0012351.1_g000003 Rmu_sc0012351.1_g000005 Rmu_sc0012915.1_g000002 Rmu_sc0013088.1_g000001 Rmu_sc0013459.1_g000001 Rmu_sc0013765.1_g000004 Rmu_sc0013765.1_g000005 Rmu_sc0014003.1_g000012 Rmu_sc0014003.1_g000014 Rmu_sc0014698.1_g000001 Rmu_sc0015740.1_g000001 Rmu_sc0016429.1_g000003 Rmu_sc0018885.1_g000001 Rmu_sc0020345.1_g000001 Rmu_sc0027791.1_g000001 Rmu_sc0037325.1_g000001 Rmu_ssc0000010.1_g000024 Rmu_ssc0000010.1_g000025 Rmu_ssc0000083.1_g000021 Rmu_ssc0000340.1_g000002 Rmu_ssc0000386.1_g000002 Rmu_ssc0000386.1_g000024 Rmu_ssc0000386.1_g000025 Rmu_ssc0000386.1_g000034 Rmu_ssc0000386.1_g000037 Rmu_ssc0000477.1_g000001
rosa_roxburghii Rroxscaffold_161G00448010 Rroxscaffold_164G00436080 Rroxscaffold_1G00024500 Rroxscaffold_2G00124290 Rroxscaffold_3G00222940 Rroxscaffold_3G00227440 Rroxscaffold_4G00304810 Rroxscaffold_4G00330180 Rroxscaffold_4G00330200 Rroxscaffold_5G00356620 Rroxscaffold_5G00358170 Rroxscaffold_5G00360880 Rroxscaffold_5G00361220 Rroxscaffold_5G00361950 Rroxscaffold_5G00361960 Rroxscaffold_5G00361990 Rroxscaffold_5G00362370 Rroxscaffold_5G00364540 Rroxscaffold_5G00364550 Rroxscaffold_5G00364580 Rroxscaffold_5G00364600 Rroxscaffold_6G00393290 Rroxscaffold_6G00412680 Rroxscaffold_6G00412790 Rroxscaffold_6G00413470 Rroxscaffold_6G00413720 Rroxscaffold_6G00416070 Rroxscaffold_7G00205400
rosa_rugosa Rorug01G0017300 Rorug01G0017400 Rorug01G0018000 Rorug01G0018100 Rorug01G0211500 Rorug02G0638200 Rorug03G0070000 Rorug03G0071200 Rorug03G0081000 Rorug03G0086500 Rorug03G0089300 Rorug03G0095300 Rorug03G0096000 Rorug03G0096000 Rorug04G0055000 Rorug04G0078300 Rorug04G0104100.1 Rorug04G0113800 Rorug04G0126700 Rorug04G0128000 Rorug04G0145900 Rorug04G0148700 Rorug04G0149100 Rorug04G0149100 Rorug04G0149200 Rorug04G0149300 Rorug04G0154900 Rorug04G0180400 Rorug04G0180500 Rorug04G0180700 Rorug04G0180800 Rorug04G0401500 Rorug04G0402100 Rorug05G0288600 Rorug05G0334100 Rorug07G0176400
rosa_samantha Rh1DG043200 Rh1DG043500 Rh1DG043900 Rh1DG222800 Rh3CG039900 Rh3CG156200 Rh3CG328500 Rh3CG328800 Rh3DG165000 Rh4AG208400 Rh4BG205600 Rh4BG213900 Rh4BG243900 Rh4CG198800 Rh4DG168600 Rh4DG180100 Rh4DG180300 Rh4DG180600 Rh4DG181200 Rh4DG181600 Rh4DG181700 Rh4DG183700 Rh4DG184000 Rh4DG184400 Rh4DG184700 Rh4DG206100 Rh4DG206200 Rh4DG206300 Rh4DG215600 Rh4DG238700 Rh4DG238900 Rh5DG390300
rosa_wichuraiana Rw0G005050 Rw0G009260 Rw0G009280 Rw0G015600 Rw0G023570 Rw1G002140 Rw1G002170 Rw1G002210 Rw1G002240 Rw1G019500 Rw2G015490 Rw3G003020 Rw3G010900 Rw3G012030 Rw3G012790 Rw3G013050 Rw3G013540 Rw3G013640 Rw3G013650 Rw3G026050 Rw3G026070 Rw4G015740 Rw4G015790 Rw4G015910 Rw4G015930 Rw4G015950 Rw4G017900 Rw4G017930 Rw4G018440 Rw4G018450 Rw4G018530 Rw4G020720 Rw4G020760 Rw5G019250 Rw5G034370 Rw7G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 290
AatII GACGTC 1 cut(s) 879
AccBSI CCGCTC 2 cut(s) 968, 2035
AccII CGCG 2 cut(s) 105, 1933
AccIII TCCGGA 2 cut(s) 2844, 2985
AcoI YGGCCR 1 cut(s) 2422
AcuI CTGAAG 6 cut(s) 198, 1080, 1331, 1856, 2759, 2900
AcyI GRCGYC 2 cut(s) 876, 3330
AfaI GTAC 7 cut(s) 9, 725, 867, 1867, 2271, 3171, 3258
AfiI CCNNNNNNNGG 5 cut(s) 32, 1415, 2326, 2420, 3266
AflII CTTAAG 1 cut(s) 1783
AflIII ACRYGT 5 cut(s) 387, 1654, 1777, 2155, 2476
AjiI CACGTC 1 cut(s) 390
AjnI CCWGG 1 cut(s) 252
Alw21I GWGCWC 1 cut(s) 2568
Aor13HI TCCGGA 2 cut(s) 2844, 2985
AoxI GGCC 8 cut(s) 363, 634, 1735, 2128, 2422, 3398, 3655, 3665
ApeKI GCWGC 7 cut(s) 164, 333, 416, 452, 455, 1154, 2696
Asp700I GAANNNNTTC 5 cut(s) 600, 1002, 2789, 3201, 3486
AspA2I CCTAGG 3 cut(s) 637, 1007, 2884
AspS9I GGNCC 7 cut(s) 364, 1047, 1736, 2087, 3308, 3656, 3666
AsuC2I CCSGG 1 cut(s) 3113
AsuHPI GGTGA 9 cut(s) 505, 622, 698, 955, 1296, 1412, 1531, 2087, 3580
AvaII GGWCC 3 cut(s) 1047, 2087, 3308
AvrII CCTAGG 3 cut(s) 637, 1007, 2884
AxyI CCTNAGG 1 cut(s) 3402
BaeI ACNNNNGTAYC 2 cut(s) 1919, 1952
BanII GRGCYC 4 cut(s) 2568, 2757, 2898, 3039
BbsI GAAGAC 3 cut(s) 1136, 2052, 3384
Bbv12I GWGCWC 1 cut(s) 2568
BbvI GCAGC 7 cut(s) 151, 345, 428, 464, 467, 1166, 2708
BceAI ACGGC 1 cut(s) 2821
BciT130I CCWGG 1 cut(s) 254
BciVI GTATCC 1 cut(s) 1597
BclI TGATCA 2 cut(s) 70, 1971
BcnI CCSGG 1 cut(s) 3113
BfmI CTRYAG 3 cut(s) 331, 1506, 3144
BfrI CTTAAG 1 cut(s) 1783
BfuI GTATCC 1 cut(s) 1597
BglII AGATCT 2 cut(s) 1855, 3688
BisI GCNGC 7 cut(s) 165, 334, 417, 453, 456, 1155, 2697
BlnI CCTAGG 3 cut(s) 637, 1007, 2884
BlsI GCNGC 7 cut(s) 166, 335, 418, 454, 457, 1156, 2698
Bme1390I CCNGG 2 cut(s) 254, 3113
Bme18I GGWCC 3 cut(s) 1047, 2087, 3308
BmgBI CACGTC 1 cut(s) 390
BmgT120I GGNCC 7 cut(s) 364, 1047, 1736, 2087, 3308, 3656, 3666
BmiI GGNNCC 5 cut(s) 101, 1351, 2286, 3038, 3310
BmrFI CCNGG 2 cut(s) 254, 3113
BpiI GAAGAC 3 cut(s) 1136, 2052, 3384
BpmI CTGGAG 2 cut(s) 815, 1502
BpuEI CTTGAG 6 cut(s) 341, 1377, 1864, 2021, 3077, 3539
BpuMI CCSGG 1 cut(s) 3113
Bsa29I ATCGAT 1 cut(s) 2718
BsaAI YACGTR 2 cut(s) 727, 2249
BsaBI GATNNNNATC 2 cut(s) 186, 2678
BsaHI GRCGYC 2 cut(s) 876, 3330
BsaJI CCNNGG 9 cut(s) 393, 637, 1007, 1158, 1931, 2588, 2884, 3111, 3645
BsaWI WCCGGW 2 cut(s) 2844, 2985
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 5 cut(s) 32, 1415, 2326, 2420, 3266
Bse1I ACTGG 4 cut(s) 459, 1191, 1485, 3658
Bse21I CCTNAGG 1 cut(s) 3402
Bse3DI GCAATG 2 cut(s) 1122, 1322
Bse8I GATNNNNATC 2 cut(s) 186, 2678
BseAI TCCGGA 2 cut(s) 2844, 2985
BseBI CCWGG 1 cut(s) 254
BseCI ATCGAT 1 cut(s) 2718
BseDI CCNNGG 9 cut(s) 393, 637, 1007, 1158, 1931, 2588, 2884, 3111, 3645
BseJI GATNNNNATC 2 cut(s) 186, 2678
BseLI CCNNNNNNNGG 5 cut(s) 32, 1415, 2326, 2420, 3266
BseMI GCAATG 2 cut(s) 1122, 1322
BseMII CTCAG 4 cut(s) 2478, 2544, 3147, 3483
BseNI ACTGG 4 cut(s) 459, 1191, 1485, 3658
BseRI GAGGAG 4 cut(s) 51, 2093, 3191, 3335
BseXI GCAGC 7 cut(s) 151, 345, 428, 464, 467, 1166, 2708
BseYI CCCAGC 1 cut(s) 2021
BsgI GTGCAG 4 cut(s) 471, 2749, 2890, 3031
Bsh1236I CGCG 2 cut(s) 105, 1933
BshFI GGCC 8 cut(s) 365, 636, 1737, 2130, 2424, 3400, 3657, 3667
BshVI ATCGAT 1 cut(s) 2718
BsiHKAI GWGCWC 1 cut(s) 2568
BsiSI CCGG 4 cut(s) 33, 2845, 2986, 3113
BslFI GGGAC 5 cut(s) 1133, 2396, 3294, 3489, 3606
BslI CCNNNNNNNGG 5 cut(s) 32, 1415, 2326, 2420, 3266
BsmBI CGTCTC 2 cut(s) 359, 2844
BsmFI GGGAC 5 cut(s) 1133, 2396, 3294, 3489, 3606
BsmI GAATGC 1 cut(s) 2396
BsnI GGCC 8 cut(s) 365, 636, 1737, 2130, 2424, 3400, 3657, 3667
Bsp1286I GDGCHC 4 cut(s) 2568, 2757, 2898, 3039
Bsp13I TCCGGA 2 cut(s) 2844, 2985
Bsp1407I TGTACA 2 cut(s) 1865, 3169
BspANI GGCC 8 cut(s) 365, 636, 1737, 2130, 2424, 3400, 3657, 3667
BspCNI CTCAG 4 cut(s) 2479, 2545, 3148, 3484
BspDI ATCGAT 1 cut(s) 2718
BspEI TCCGGA 2 cut(s) 2844, 2985
BspFNI CGCG 2 cut(s) 105, 1933
BspHI TCATGA 1 cut(s) 1621
BspLI GGNNCC 5 cut(s) 101, 1351, 2286, 3038, 3310
BspMAI CTGCAG 1 cut(s) 335
BspQI GCTCTTC 2 cut(s) 3492, 3611
BspTI CTTAAG 1 cut(s) 1783
BsrBI CCGCTC 2 cut(s) 968, 2035
BsrDI GCAATG 2 cut(s) 1122, 1322
BsrGI TGTACA 2 cut(s) 1865, 3169
BsrI ACTGG 4 cut(s) 459, 1191, 1485, 3658
BssECI CCNNGG 9 cut(s) 393, 637, 1007, 1158, 1931, 2588, 2884, 3111, 3645
BssNI GRCGYC 2 cut(s) 876, 3330
BssT1I CCWWGG 6 cut(s) 393, 637, 1007, 1158, 2884, 3645
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 1 cut(s) 535
BstACI GRCGYC 2 cut(s) 876, 3330
BstAFI CTTAAG 1 cut(s) 1783
BstAUI TGTACA 2 cut(s) 1865, 3169
BstBAI YACGTR 2 cut(s) 727, 2249
BstC8I GCNNGC 9 cut(s) 367, 414, 421, 658, 813, 1832, 1901, 2188, 2834
BstDEI CTNAG 8 cut(s) 1185, 2191, 2487, 2553, 3156, 3382, 3402, 3492
BstDSI CCRYGG 1 cut(s) 1931
BstEII GGTNACC 1 cut(s) 1418
BstFNI CGCG 2 cut(s) 105, 1933
BstMWI GCNNNNNNNGC 6 cut(s) 62, 239, 330, 1091, 2183, 3397
BstNI CCWGG 1 cut(s) 254
BstNSI RCATGY 5 cut(s) 8, 1658, 1781, 2159, 2836
BstPI GGTNACC 1 cut(s) 1418
BstSCI CCNGG 2 cut(s) 252, 3111
BstSFI CTRYAG 3 cut(s) 331, 1506, 3144
BstSNI TACGTA 2 cut(s) 727, 2249
BstUI CGCG 2 cut(s) 105, 1933
BstV1I GCAGC 7 cut(s) 151, 345, 428, 464, 467, 1166, 2708
BstV2I GAAGAC 3 cut(s) 1136, 2052, 3384
BstX2I RGATCY 7 cut(s) 187, 400, 974, 1075, 1855, 3061, 3688
BstXI CCANNNNNNTGG 1 cut(s) 1165
BstYI RGATCY 7 cut(s) 187, 400, 974, 1075, 1855, 3061, 3688
Bsu15I ATCGAT 1 cut(s) 2718
Bsu36I CCTNAGG 1 cut(s) 3402
BsuI GTATCC 1 cut(s) 1597
BsuRI GGCC 8 cut(s) 365, 636, 1737, 2130, 2424, 3400, 3657, 3667
BsuTUI ATCGAT 1 cut(s) 2718
BtgI CCRYGG 1 cut(s) 1931
BtgZI GCGATG 1 cut(s) 2677
BtrI CACGTC 1 cut(s) 390
BtsIMutI CAGTG 2 cut(s) 531, 1307
Cac8I GCNNGC 9 cut(s) 367, 414, 421, 658, 813, 1832, 1901, 2188, 2834
CciI TCATGA 1 cut(s) 1621
Cfr13I GGNCC 7 cut(s) 364, 1047, 1736, 2087, 3308, 3656, 3666
Cfr42I CCGCGG 1 cut(s) 1934
ClaI ATCGAT 1 cut(s) 2718
CseI GACGC 1 cut(s) 3338
Csp6I GTAC 7 cut(s) 8, 724, 866, 1866, 2270, 3170, 3257
CviQI GTAC 7 cut(s) 8, 724, 866, 1866, 2270, 3170, 3257
DdeI CTNAG 8 cut(s) 1185, 2191, 2487, 2553, 3156, 3382, 3402, 3492
DraI TTTAAA 1 cut(s) 3447
EaeI YGGCCR 1 cut(s) 2422
EciI GGCGGA 1 cut(s) 3195
Ecl136II GAGCTC 1 cut(s) 2566
Eco105I TACGTA 2 cut(s) 727, 2249
Eco130I CCWWGG 6 cut(s) 393, 637, 1007, 1158, 2884, 3645
Eco147I AGGCCT 1 cut(s) 636
Eco24I GRGCYC 4 cut(s) 2568, 2757, 2898, 3039
Eco32I GATATC 1 cut(s) 88
Eco47I GGWCC 3 cut(s) 1047, 2087, 3308
Eco53kI GAGCTC 1 cut(s) 2566
Eco57I CTGAAG 6 cut(s) 198, 1080, 1331, 1856, 2759, 2900
Eco81I CCTNAGG 1 cut(s) 3402
Eco91I GGTNACC 1 cut(s) 1418
EcoICRI GAGCTC 1 cut(s) 2566
EcoO109I RGGNCCY 1 cut(s) 2087
EcoO65I GGTNACC 1 cut(s) 1418
EcoRI GAATTC 1 cut(s) 509
EcoRII CCWGG 1 cut(s) 252
EcoRV GATATC 1 cut(s) 88
EcoT14I CCWWGG 6 cut(s) 393, 637, 1007, 1158, 2884, 3645
EcoT22I ATGCAT 2 cut(s) 6, 1557
EcoT38I GRGCYC 4 cut(s) 2568, 2757, 2898, 3039
ErhI CCWWGG 6 cut(s) 393, 637, 1007, 1158, 2884, 3645
Esp3I CGTCTC 2 cut(s) 359, 2844
FalI AAGNNNNNCTT 8 cut(s) 2044, 2076, 2174, 2206, 2192, 2224, 3590, 3622
FaqI GGGAC 5 cut(s) 1133, 2396, 3294, 3489, 3606
FauI CCCGC 1 cut(s) 374
FauNDI CATATG 1 cut(s) 3097
FbaI TGATCA 2 cut(s) 70, 1971
Fnu4HI GCNGC 7 cut(s) 165, 334, 417, 453, 456, 1155, 2697
FriOI GRGCYC 4 cut(s) 2568, 2757, 2898, 3039
Fsp4HI GCNGC 7 cut(s) 165, 334, 417, 453, 456, 1155, 2697
GluI GCNGC 7 cut(s) 165, 334, 417, 453, 456, 1155, 2697
GsaI CCCAGC 1 cut(s) 2025
GsuI CTGGAG 2 cut(s) 815, 1502
HaeIII GGCC 8 cut(s) 365, 636, 1737, 2130, 2424, 3400, 3657, 3667
HapII CCGG 4 cut(s) 33, 2845, 2986, 3113
HgaI GACGC 1 cut(s) 3338
Hin1I GRCGYC 2 cut(s) 876, 3330
HincII GTYRAC 2 cut(s) 2040, 2220
HindII GTYRAC 2 cut(s) 2040, 2220
HindIII AAGCTT 3 cut(s) 1171, 1763, 2305
HpaI GTTAAC 1 cut(s) 2040
HpaII CCGG 4 cut(s) 33, 2845, 2986, 3113
HphI GGTGA 9 cut(s) 505, 622, 698, 955, 1296, 1412, 1531, 2087, 3580
Hpy166II GTNNAC 3 cut(s) 724, 2040, 2220
Hpy8I GTNNAC 3 cut(s) 724, 2040, 2220
Hpy99I CGWCG 2 cut(s) 2618, 2621
HpyCH4III ACNGT 1 cut(s) 535
HpyCH4IV ACGT 5 cut(s) 389, 726, 876, 2248, 2476
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 239, 330, 1091, 2183, 3397
HpyF3I CTNAG 8 cut(s) 1185, 2191, 2487, 2553, 3156, 3382, 3402, 3492
HpySE526I ACGT 5 cut(s) 389, 726, 876, 2248, 2476
Hsp92I GRCGYC 2 cut(s) 876, 3330
Kpn2I TCCGGA 2 cut(s) 2844, 2985
Ksp22I TGATCA 2 cut(s) 70, 1971
KspAI GTTAAC 1 cut(s) 2040
KspI CCGCGG 1 cut(s) 1934
LguI GCTCTTC 2 cut(s) 3492, 3611
LmnI GCTCC 7 cut(s) 1090, 1337, 2032, 2483, 2988, 3042, 3322
Lsp1109I GCAGC 7 cut(s) 151, 345, 428, 464, 467, 1166, 2708
MaeII ACGT 5 cut(s) 389, 726, 876, 2248, 2476
MaeIII GTNAC 6 cut(s) 784, 961, 1418, 2204, 2294, 2657
MbiI CCGCTC 2 cut(s) 968, 2035
MfeI CAATTG 7 cut(s) 1356, 2120, 2398, 2708, 2780, 2794, 3303
MflI RGATCY 7 cut(s) 187, 400, 974, 1075, 1855, 3061, 3688
MhlI GDGCHC 4 cut(s) 2568, 2757, 2898, 3039
MlyI GAGTC 8 cut(s) 75, 438, 1468, 2212, 2365, 2418, 2678, 3110
MmeI TCCRAC 7 cut(s) 517, 609, 822, 1269, 1402, 1700, 2843
Mph1103I ATGCAT 2 cut(s) 6, 1557
MroI TCCGGA 2 cut(s) 2844, 2985
MroXI GAANNNNTTC 5 cut(s) 600, 1002, 2789, 3201, 3486
MslI CAYNNNNRTG 2 cut(s) 2227, 3100
MspA1I CMGCKG 2 cut(s) 419, 1933
MspCI CTTAAG 1 cut(s) 1783
MspI CCGG 4 cut(s) 33, 2845, 2986, 3113
MspR9I CCNGG 2 cut(s) 254, 3113
MunI CAATTG 7 cut(s) 1356, 2120, 2398, 2708, 2780, 2794, 3303
Mva1269I GAATGC 1 cut(s) 2396
MvaI CCWGG 1 cut(s) 254
MvnI CGCG 2 cut(s) 105, 1933
MwoI GCNNNNNNNGC 6 cut(s) 62, 239, 330, 1091, 2183, 3397
NciI CCSGG 1 cut(s) 3113
NdeI CATATG 1 cut(s) 3097
NlaIV GGNNCC 5 cut(s) 101, 1351, 2286, 3038, 3310
NmeAIII GCCGAG 5 cut(s) 229, 300, 1806, 2400, 2585
NmuCI GTSAC 5 cut(s) 784, 961, 1418, 2204, 2657
NsiI ATGCAT 2 cut(s) 6, 1557
NspI RCATGY 5 cut(s) 8, 1658, 1781, 2159, 2836
PaeI GCATGC 1 cut(s) 2836
PagI TCATGA 1 cut(s) 1621
PceI AGGCCT 1 cut(s) 636
PciI ACATGT 3 cut(s) 1654, 1777, 2155
PciSI GCTCTTC 2 cut(s) 3492, 3611
PcsI WCGNNNNNNNCGW 3 cut(s) 2585, 3128, 3283
PctI GAATGC 1 cut(s) 2396
PdmI GAANNNNTTC 5 cut(s) 600, 1002, 2789, 3201, 3486
PfeI GAWTC 7 cut(s) 128, 1666, 2377, 3124, 3197, 3365, 3547
PfoI TCCNGGA 1 cut(s) 252
PkrI GCNGC 7 cut(s) 166, 335, 418, 454, 457, 1156, 2698
PleI GAGTC 8 cut(s) 75, 437, 1468, 2211, 2364, 2417, 2677, 3110
PpsI GAGTC 8 cut(s) 75, 437, 1468, 2211, 2364, 2417, 2677, 3110
Ppu21I YACGTR 2 cut(s) 727, 2249
PpuMI RGGWCCY 1 cut(s) 2087
PscI ACATGT 3 cut(s) 1654, 1777, 2155
PsiI TTATAA 1 cut(s) 290
Psp124BI GAGCTC 1 cut(s) 2568
Psp5II RGGWCCY 1 cut(s) 2087
Psp6I CCWGG 1 cut(s) 252
PspEI GGTNACC 1 cut(s) 1418
PspFI CCCAGC 1 cut(s) 2021
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 5 cut(s) 101, 1351, 2286, 3038, 3310
PspPI GGNCC 7 cut(s) 364, 1047, 1736, 2087, 3308, 3656, 3666
PspPPI RGGWCCY 1 cut(s) 2087
PstI CTGCAG 1 cut(s) 335
PsuI RGATCY 7 cut(s) 187, 400, 974, 1075, 1855, 3061, 3688
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 7 cut(s) 9, 725, 867, 1867, 2271, 3171, 3258
RsaNI GTAC 7 cut(s) 8, 724, 866, 1866, 2270, 3170, 3257
RseI CAYNNNNRTG 2 cut(s) 2227, 3100
SacI GAGCTC 1 cut(s) 2568
SacII CCGCGG 1 cut(s) 1934
SapI GCTCTTC 2 cut(s) 3492, 3611
SatI GCNGC 7 cut(s) 165, 334, 417, 453, 456, 1155, 2697
Sau96I GGNCC 7 cut(s) 364, 1047, 1736, 2087, 3308, 3656, 3666
SchI GAGTC 8 cut(s) 75, 438, 1468, 2212, 2365, 2418, 2678, 3110
ScrFI CCNGG 2 cut(s) 254, 3113
SduI GDGCHC 4 cut(s) 2568, 2757, 2898, 3039
SfcI CTRYAG 3 cut(s) 331, 1506, 3144
Sfr303I CCGCGG 1 cut(s) 1934
SgrBI CCGCGG 1 cut(s) 1934
SinI GGWCC 3 cut(s) 1047, 2087, 3308
SmiMI CAYNNNNRTG 2 cut(s) 2227, 3100
SmlI CTYRAG 7 cut(s) 356, 1392, 1783, 1843, 2000, 3056, 3518
SmoI CTYRAG 7 cut(s) 356, 1392, 1783, 1843, 2000, 3056, 3518
SnaBI TACGTA 2 cut(s) 727, 2249
SphI GCATGC 1 cut(s) 2836
SseBI AGGCCT 1 cut(s) 636
SspI AATATT 2 cut(s) 373, 601
SstI GAGCTC 1 cut(s) 2568
StuI AGGCCT 1 cut(s) 636
StyD4I CCNGG 2 cut(s) 252, 3111
StyI CCWWGG 6 cut(s) 393, 637, 1007, 1158, 2884, 3645
TaaI ACNGT 1 cut(s) 535
TaiI ACGT 5 cut(s) 392, 729, 879, 2251, 2479
TatI WGTACW 5 cut(s) 7, 865, 1865, 2269, 3169
TfiI GAWTC 7 cut(s) 128, 1666, 2377, 3124, 3197, 3365, 3547
TscAI CASTG 2 cut(s) 538, 1314
TseFI GTSAC 5 cut(s) 784, 961, 1418, 2204, 2657
TseI GCWGC 7 cut(s) 164, 333, 416, 452, 455, 1154, 2696
Tsp45I GTSAC 5 cut(s) 784, 961, 1418, 2204, 2657
TspGWI ACGGA 5 cut(s) 1678, 2279, 2331, 2594, 2693
TspRI CASTG 2 cut(s) 538, 1314
Vha464I CTTAAG 1 cut(s) 1783
VpaK11BI GGWCC 3 cut(s) 1047, 2087, 3308
XbaI TCTAGA 3 cut(s) 1216, 1852, 3193
XceI RCATGY 5 cut(s) 8, 1658, 1781, 2159, 2836
XcmI CCANNNNNNNNNTGG 1 cut(s) 1412
XmaJI CCTAGG 3 cut(s) 637, 1007, 2884
XmnI GAANNNNTTC 5 cut(s) 600, 1002, 2789, 3201, 3486
ZraI GACGTC 1 cut(s) 877
Zsp2I ATGCAT 2 cut(s) 6, 1557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.