AT2G04300

leucine-rich repeat protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000083
Physical Location & Seq
Forward (+)
1493009 .. 1497016
4008 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G04300.2

Sequence Viewer

Length: 2556 bp
ATGAAAACACATCCTCAAGCAATTCTCTTATGTGTGTTATTCTTCATCACGTTTGGTCTTTTACATGTCGTTGAAGCTGGAAATCAAGAAGGATTCATCAGTTTAGATTGTGGGTTATCCCCCAATGAACCTCCTTACGTCGATGCTGCAACCGACTTAACATACACAACGGACAATGATTTCGTGCAGAGCGGTAAAACTGGTACAATCGATAAGGAATTGGAGTCAACCTACAACAAACCAATTTTACAGCTTAGGTACTTCCCCGAAGGAGTCCGAAACTGTTATACCTTGAACGTCACGCTCGGCACAAACTACCTGATCAGAGCCAGTTTCGTGTATGGTAACTACGATGGTCTTAATAAAGAACTCGAGTTTGACCTTTACCTTGGTCCTAATCTATGGGCAAACGTGAACACAGCTGTATATTTAATGAACGGAGTGACCACAGAAGAAATCATCCACAGTACCAAATCTAAGGTACTCCAGGTTTGTCTTATTAAGACAGGCGAGAGTATACCTATTATTAATAGCTTAGAGCTGCGACCACTTATAAACGATACTTACAATACTCAAAGTGGCTCGCTGAAATACTTATTTCGGAATTATTTCAGCACTTCAAGGAGAATAATACGGTACCCGAATGATGTCAACGATCGTCATTGGTATCCGTTCTTTGATGAGGATGCGTGGACAGAATTGACTACAAATCTCAATGTTAACAGTTCAAATGGTTATGATCCACCAAAATTTGTAATGGCTTCAGCCTCAACACCCATAAGTAAAAATGCGCCCTTCAACTTCACCTGGTCATTGATTCCTTCTACGGCCAAATTTTATAGTTACATGCACTTCGCCGATATTCAGACTCTACAGGCCAATGAAACCCGAGAATTCGACATGATGTTGAATGGAAACCTTGCCTTGGAACGTGCCCTCGAGGTTTTCACCGTGATCGATTTCCCCGAATTGGAAACAAATCAAGATGATGTTATTGCTATCAAGAATATCCAAAATACTTATGGAGTGAGTAAAACTAGCTGGCAAGGAGATCCATGTGTTCCTAAACGGTTTATGTGGGATGGCTTAAACTGCAACAACTCGTATATTTCCACACCACCTACAATAACTTTTTTAAACCTATCATCAAGTCATTTAACGGGGATCATTGCATCTGCCATTCAAAACCTAACCCACCTGCAAAATTTGGACTTGTCAAATAACAATTTGACAGGAGGAGTACCCGAGTTTCTTGCTGGCTTAAAATCACTCTTAGTCATAAACTTAAGTGGGAATAATCTTAGTGGTTCTGTTCCTCAAACCCTTCTCCAGAAGAAAGGACTTAAGTTAAATCTTGAAGGAAATATTTATCTTAATTGTCCGGATGGATCATGTGTAAGCAAAGACGGAAATGGAGGTGCCAAGAAAAAGAATGTTGTAGTATTGGTTGTGGTATCAATTGCACTTGTAGTAGTTCTTGGATCTGCATTAGCTCTTTTTTTGGTGTTTAGAAAAAGAAAAACACCACGCAATGAAGTTTCTAGAACATCTAGATCATTAGACCCGACAATAACGACGAAAAACAGAAGATTTACTTATTCGGAAGTTGTAAAGATGACAAATAATTTTGAGAAAATCCTTGGTAAAGGAGGGTTTGGAATGGTCTATCATGGAACTGTGAATGATGCTGAACAAGTAGCCGTTAAAATGTTATCACCCTCATCATCTCAAGGGTATAAAGAATTCAAAGCAGAGGTAGAACTCCTTCTCAGAGTTCACCATAAAAATTTGGTTGGCCTCGTTGGATATTGTGATGAAGGAGAAAATTTATCTCTCATCTACGAGTACATGGCTAAAGGAGATCTTAAAGAACATATGTTAGGAAACCAAGGTGTATCTATTTTGGACTGGAAAACTAGACTAAAGATAGTGGCCGAGTCCGCGCAAGGGCTGGAATACTTGCATAATGGATGCAAACCACCAATGGTACATAGAGATGTCAAAACCACAAATATATTGTTGGATGAACATTTTCAGGCCAAGCTTGCTGATTTCGGTCTTTCGAGATCTTTTCCTCTTGAAGGAGAAACCCGTGTGGACACAGTTGTTGCTGGAACTCCTGGGTACCTTGATCCAGAATATTATCGAACAAATTGGTTGAACGAGAAAAGTGATGTTTATAGCTTTGGAATCGTACTATTAGAGATCATCACAAACCAACATGTGATCAACCAAAGTCGTGAAAAACCACATATAGCTGAATGGGTTGGGGTGATGCTTACAAAAGGAGACATCAAAAGCATTATAGATCCAAAATTTAGTGGAGATTATGATGCTGGTTCTGTCTGGAGAGCAGTTGAACTAGCAATGTCGTGTGTAAATCCTTCTTCAACTGGAAGACCAACCATGTCTCAAGTTGTAATCGAATTAAATGAATGTTTGGCATCAGAAAACTCAAGGAGAGGAATGAGTCAAAACATGGAGTCAAAGGGATCTATCCAATATACAGAAGTCAGCACGAACTTTGGTACTGAATATACCCCTGAAGCTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001653 GO:0002229 GO:0002237 GO:0002238 GO:0002239 GO:0002376 GO:0002831 GO:0002832 GO:0002833 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009787 GO:0009788 GO:0009790 GO:0009791 GO:0009793 GO:0009845 GO:0009888 GO:0009960 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010154 GO:0010200 GO:0010243 GO:0010646 GO:0010648 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023051 GO:0023052 GO:0023057 GO:0031347 GO:0031348 GO:0031349 GO:0032101 GO:0032102 GO:0032103 GO:0032501 GO:0032502 GO:0032879 GO:0036211 GO:0038023 GO:0042221 GO:0042493 GO:0042802 GO:0042803 GO:0042995 GO:0043170 GO:0043207 GO:0043412 GO:0043900 GO:0043901 GO:0043902 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0046983 GO:0048316 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0090351 GO:0090406 GO:0098542 GO:0120025 GO:0140096 GO:1900150 GO:1900424 GO:1900426 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901700 GO:1902288 GO:1902289 GO:1905957 GO:1905958 GO:2000071
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

851

Amino Acids

94.99

Weight (kDa)

5.76

Isoelectric Point (pI)

32.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 35 - 359 6.7e-121 Malectin-like domain
LRR_8 PF13855 417 - 475 1.2e-08 Leucine rich repeat
LRR_4 PF12799 439 - 478 9.9e-06 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 584 - 848 4.4e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 584 - 848 1e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000083)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07560 AT1G51790 AT1G51790 AT1G51790 AT1G51800 AT1G51805 AT1G51805 AT1G51810 AT1G51820 AT1G51820 AT1G51830 AT1G51830 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51850 AT1G51850 AT1G51860 AT1G51860 AT1G51870 AT1G51870 AT1G51880 AT1G51880 AT1G51880 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51910 AT2G04300 AT2G04300 AT2G28960 AT2G28960 AT2G28970 AT2G28970 AT2G28990 AT2G28990 AT2G29000 AT3G21340 AT3G46350 AT5G59616 AT5G59650 AT5G59650 AT5G59660 AT5G59660 AT5G59660 AT5G59670 AT5G59670 AT5G59680
fragaria_vesca FvH4_2g03270 FvH4_3g21300 FvH4_6g32660 FvH4_6g32660 FvH4_6g32670 FvH4_6g32670 FvH4_6g32690 FvH4_6g32690 FvH4_6g32691 FvH4_6g32700 FvH4_6g32740 FvH4_6g32750 FvH4_6g33210 FvH4_6g33210 FvH4_7g24270 FvH4_7g24280 FvH4_7g24291 FvH4_7g24300
malus_domestica MD09G1198400.v1.1 MD09G1198600.v1.1 MD09G1199100.v1.1 MD09G1199200.v1.1 MD09G1199300.v1.1 MD09G1199500.v1.1 MD09G1199600.v1.1 MD17G1179300.v1.1 MD17G1180200.v1.1 MD17G1266000.v1.1
prunus_persica Prupe.1G438900_v2.0.a1 Prupe.1G439000_v2.0.a1 Prupe.1G439100_v2.0.a1 Prupe.3G019400_v2.0.a1 Prupe.3G021900_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G055100_v2.0.a1 Prupe.3G055400_v2.0.a1 Prupe.3G055500_v2.0.a1 Prupe.3G055600_v2.0.a1 Prupe.3G055700_v2.0.a1 Prupe.3G055800_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056100_v2.0.a1 Prupe.3G209600_v2.0.a1 Prupe.3G209700_v2.0.a1 Prupe.6G291100_v2.0.a1 Prupe.7G208900_v2.0.a1 Prupe.8G038300_v2.0.a1
pyrus_communis pycom09g11570 pycom09g11580 pycom09g11610 pycom09g11630 pycom09g11640 pycom17g18840 pycom17g18910 pycom17g18930 pycom17g18940
rosa_chinensis RchiOBHm_Chr1g0317521 RchiOBHm_Chr1g0317541 RchiOBHm_Chr2g0123351 RchiOBHm_Chr2g0141181 RchiOBHm_Chr2g0141191 RchiOBHm_Chr2g0141211 RchiOBHm_Chr2g0141221 RchiOBHm_Chr2g0141261 RchiOBHm_Chr2g0141271 RchiOBHm_Chr2g0141311 RchiOBHm_Chr2g0141331 RchiOBHm_Chr2g0141371 RchiOBHm_Chr2g0141391 RchiOBHm_Chr2g0141401 RchiOBHm_Chr2g0141431 RchiOBHm_Chr2g0141481 RchiOBHm_Chr2g0141491 RchiOBHm_Chr2g0141591 RchiOBHm_Chr2g0141601 RchiOBHm_Chr2g0141611 RchiOBHm_Chr2g0141631 RchiOBHm_Chr2g0141641 RchiOBHm_Chr2g0141661 RchiOBHm_Chr2g0141681 RchiOBHm_Chr2g0141701 RchiOBHm_Chr2g0141781 RchiOBHm_Chr2g0141811 RchiOBHm_Chr2g0141821 RchiOBHm_Chr3g0479311 RchiOBHm_Chr3g0495751 RchiOBHm_Chr4g0404741 RchiOBHm_Chr4g0410341 RchiOBHm_Chr4g0418371 RchiOBHm_Chr4g0438651 RchiOBHm_Chr5g0036411 RchiOBHm_Chr5g0036491 RchiOBHm_Chr5g0042391 RchiOBHm_Chr6g0279871 RchiOBHm_Chr7g0192921 RchiOBHm_Chr7g0214901 RchiOBHm_Chr7g0234931
rosa_laevigata RLG00000003432 RLG00000008806 RLG00000013062 RLG00000019514 RLG00000019923 RLG00000019924 RLG00000019925 RLG00000019930 RLG00000019931 RLG00000019934 RLG00000019937 RLG00000019938 RLG00000033697
rosa_multiflora Rmu_co8002302.1_g000001 Rmu_sc0000147.1_g000031 Rmu_sc0000463.1_g000005 Rmu_sc0000712.1_g000003 Rmu_sc0001483.1_g000017 Rmu_sc0001554.1_g000002 Rmu_sc0001554.1_g000005 Rmu_sc0001554.1_g000008 Rmu_sc0001981.1_g000017 Rmu_sc0002040.1_g000056 Rmu_sc0002755.1_g000018 Rmu_sc0002804.1_g000016 Rmu_sc0002928.1_g000015 Rmu_sc0003040.1_g000008 Rmu_sc0003040.1_g000018 Rmu_sc0004512.1_g000001 Rmu_sc0004512.1_g000004 Rmu_sc0005507.1_g000001 Rmu_sc0005507.1_g000009 Rmu_sc0005507.1_g000033 Rmu_sc0005507.1_g000037 Rmu_sc0005507.1_g000043 Rmu_sc0005507.1_g000047 Rmu_sc0006318.1_g000004 Rmu_sc0006318.1_g000014 Rmu_sc0006318.1_g000021 Rmu_sc0006556.1_g000001 Rmu_sc0007208.1_g000002 Rmu_sc0007208.1_g000003 Rmu_sc0007208.1_g000006 Rmu_sc0007208.1_g000008
rosa_roxburghii Rroxscaffold_1G00044430 Rroxscaffold_2G00103420 Rroxscaffold_2G00103470 Rroxscaffold_2G00103490 Rroxscaffold_2G00103500 Rroxscaffold_2G00103520 Rroxscaffold_2G00103530 Rroxscaffold_2G00103540 Rroxscaffold_2G00103550 Rroxscaffold_2G00103570 Rroxscaffold_2G00103770 Rroxscaffold_2G00103780 Rroxscaffold_2G00103800 Rroxscaffold_2G00103820 Rroxscaffold_2G00103860 Rroxscaffold_2G00103890 Rroxscaffold_2G00103930 Rroxscaffold_2G00103940 Rroxscaffold_2G00104010 Rroxscaffold_2G00104030 Rroxscaffold_2G00104050 Rroxscaffold_2G00104060 Rroxscaffold_2G00104070 Rroxscaffold_2G00104090 Rroxscaffold_2G00104100 Rroxscaffold_2G00104120 Rroxscaffold_2G00104130 Rroxscaffold_4G00331710
rosa_rugosa Rorug02G0367500 Rorug02G0367500 Rorug02G0367600 Rorug02G0367800 Rorug02G0367800 Rorug02G0367900 Rorug02G0368100 Rorug02G0368100 Rorug02G0368100 Rorug02G0368200 Rorug02G0368700 Rorug02G0368900 Rorug05G0155600 Rorug05G0155700 Rorug05G0155800 Rorug05G0156700 Rorug05G0157300
rosa_samantha Rh1AG028000 Rh2AG418700 Rh2AG418900 Rh2AG419000 Rh2AG419100 Rh2AG419400 Rh2AG419600 Rh2AG419800 Rh2AG420400 Rh2AG421000 Rh2BG428500 Rh2BG428600 Rh2BG428800 Rh2BG428900 Rh2BG429300 Rh2BG429400 Rh2BG429700 Rh2BG429900 Rh2BG430000 Rh2BG430700 Rh2CG405100 Rh2CG405200 Rh2CG405300 Rh2CG405500 Rh2CG405600 Rh2CG405700 Rh2CG406000 Rh2CG406100 Rh2CG406300 Rh2CG406600 Rh2CG406700 Rh2CG407500 Rh2CG408100 Rh2DG438500 Rh3DG123900 Rh3DG124000 Rh5BG249700 Rh5BG482500 Rh5CG281500 Rh5CG507300 Rh7DG133300
rosa_wichuraiana Rw0G019720 Rw0G019730 Rw0G019750 Rw0G023010 Rw2G034340 Rw2G034350 Rw2G034370 Rw2G034380 Rw2G034400 Rw2G034430 Rw2G034470 Rw2G034480 Rw2G034510 Rw5G022890 Rw5G026410 Rw5G032710 Rw5G042850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
AarI CACCTGC 1 cut(s) 1206
AbsI CCTCGAGG 1 cut(s) 938
Acc36I ACCTGC 1 cut(s) 1206
Acc65I GGTACC 2 cut(s) 636, 2124
AccB1I GGYRCC 3 cut(s) 636, 1418, 2124
AccBSI CCGCTC 1 cut(s) 192
AccI GTMKAC 1 cut(s) 517
AccII CGCG 1 cut(s) 1943
AccIII TCCGGA 1 cut(s) 1381
AciI CCGC 2 cut(s) 192, 1941
AclWI GGATC 8 cut(s) 734, 1046, 1172, 1396, 1489, 2126, 2303, 2500
AcoI YGGCCR 2 cut(s) 828, 1932
AcsI RAATTY 8 cut(s) 749, 833, 893, 1204, 1742, 1786, 1825, 2315
AcuI CTGAAG 1 cut(s) 747
AfiI CCNNNNNNNGG 4 cut(s) 925, 970, 1947, 2081
AflII CTTAAG 2 cut(s) 1285, 1343
AflIII ACRYGT 2 cut(s) 64, 2221
AjnI CCWGG 3 cut(s) 486, 806, 2119
Alw26I GTCTC 2 cut(s) 2283, 2415
AlwI GGATC 8 cut(s) 734, 1046, 1172, 1396, 1489, 2126, 2303, 2500
Aor13HI TCCGGA 1 cut(s) 1381
AoxI GGCC 5 cut(s) 828, 876, 1795, 1932, 2037
ApeKI GCWGC 2 cut(s) 146, 541
ApoI RAATTY 8 cut(s) 749, 833, 893, 1204, 1742, 1786, 1825, 2315
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
AseI ATTAAT 1 cut(s) 528
Asp700I GAANNNNTTC 3 cut(s) 608, 1764, 2031
Asp718I GGTACC 2 cut(s) 636, 2124
AspLEI GCGC 2 cut(s) 793, 1945
AspS9I GGNCC 1 cut(s) 392
AsuHPI GGTGA 5 cut(s) 796, 940, 1707, 1769, 2284
AvaI CYCGRG 4 cut(s) 371, 888, 938, 1244
AvaII GGWCC 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 937
BanI GGYRCC 3 cut(s) 636, 1418, 2124
BarI GAAGNNNNNNTAC 2 cut(s) 1749, 1781
BbsI GAAGAC 1 cut(s) 2404
BbvI GCAGC 2 cut(s) 133, 528
BccI CCATC 3 cut(s) 347, 1076, 1379
BceAI ACGGC 2 cut(s) 843, 1685
BcgI CGANNNNNNTGC 2 cut(s) 1235, 1269
BciT130I CCWGG 3 cut(s) 488, 808, 2121
BciVI GTATCC 1 cut(s) 678
BclI TGATCA 2 cut(s) 321, 2226
BcoDI GTCTC 2 cut(s) 2283, 2415
BfaI CTAG 6 cut(s) 1038, 1542, 1551, 1917, 2363, 2554
BfmI CTRYAG 1 cut(s) 872
BfrI CTTAAG 2 cut(s) 1285, 1343
BfuAI ACCTGC 1 cut(s) 1206
BfuI GTATCC 1 cut(s) 678
BglII AGATCT 2 cut(s) 1861, 2066
BisI GCNGC 2 cut(s) 147, 542
BlsI GCNGC 2 cut(s) 148, 543
Bme1390I CCNGG 3 cut(s) 488, 808, 2121
Bme18I GGWCC 1 cut(s) 392
BmeT110I CYCGRG 4 cut(s) 371, 888, 938, 1244
BmgT120I GGNCC 1 cut(s) 392
BmiI GGNNCC 3 cut(s) 638, 1420, 2126
BmrFI CCNGG 3 cut(s) 488, 808, 2121
BmsI GCATC 8 cut(s) 133, 676, 1181, 1675, 1961, 2265, 2323, 2453
BpiI GAAGAC 1 cut(s) 2404
BpmI CTGGAG 3 cut(s) 470, 1313, 2368
Bpu10I CCTNAGC 1 cut(s) 254
BpuEI CTTGAG 3 cut(s) 1713, 2397, 2440
Bsa29I ATCGAT 2 cut(s) 210, 957
BsaJI CCNNGG 5 cut(s) 388, 924, 1639, 1888, 2120
BsaWI WCCGGW 1 cut(s) 1381
Bsc4I CCNNNNNNNGG 4 cut(s) 925, 970, 1947, 2081
Bse1I ACTGG 4 cut(s) 205, 330, 1913, 2398
Bse3DI GCAATG 3 cut(s) 1167, 1537, 2373
BseAI TCCGGA 1 cut(s) 1381
BseBI CCWGG 3 cut(s) 488, 808, 2121
BseCI ATCGAT 2 cut(s) 210, 957
BseDI CCNNGG 5 cut(s) 388, 924, 1639, 1888, 2120
BseGI GGATG 7 cut(s) 10, 459, 691, 1087, 1390, 1976, 2029
BseLI CCNNNNNNNGG 4 cut(s) 925, 970, 1947, 2081
BseMI GCAATG 3 cut(s) 1167, 1537, 2373
BseMII CTCAG 1 cut(s) 1783
BseNI ACTGG 4 cut(s) 205, 330, 1913, 2398
BseRI GAGGAG 1 cut(s) 1251
BseSI GKGCMC 1 cut(s) 937
BseXI GCAGC 2 cut(s) 133, 528
BsgI GTGCAG 1 cut(s) 206
Bsh1236I CGCG 1 cut(s) 1943
Bsh1285I CGRYCG 1 cut(s) 658
BshFI GGCC 5 cut(s) 830, 878, 1797, 1934, 2039
BshNI GGYRCC 3 cut(s) 636, 1418, 2124
BshVI ATCGAT 2 cut(s) 210, 957
BsiEI CGRYCG 1 cut(s) 658
BsiHKCI CYCGRG 4 cut(s) 371, 888, 938, 1244
BsiSI CCGG 1 cut(s) 1382
BslI CCNNNNNNNGG 4 cut(s) 925, 970, 1947, 2081
BsmAI GTCTC 2 cut(s) 2283, 2415
BsnI GGCC 5 cut(s) 830, 878, 1797, 1934, 2039
BsoBI CYCGRG 4 cut(s) 371, 888, 938, 1244
Bsp1286I GDGCHC 1 cut(s) 937
Bsp13I TCCGGA 1 cut(s) 1381
BspACI CCGC 2 cut(s) 192, 1941
BspANI GGCC 5 cut(s) 830, 878, 1797, 1934, 2039
BspCNI CTCAG 1 cut(s) 1782
BspDI ATCGAT 2 cut(s) 210, 957
BspEI TCCGGA 1 cut(s) 1381
BspFNI CGCG 1 cut(s) 1943
BspLI GGNNCC 3 cut(s) 638, 1420, 2126
BspMI ACCTGC 1 cut(s) 1206
BspPI GGATC 8 cut(s) 734, 1046, 1172, 1396, 1489, 2126, 2303, 2500
BspT107I GGYRCC 3 cut(s) 636, 1418, 2124
BspTI CTTAAG 2 cut(s) 1285, 1343
BsrBI CCGCTC 1 cut(s) 192
BsrDI GCAATG 3 cut(s) 1167, 1537, 2373
BsrI ACTGG 4 cut(s) 205, 330, 1913, 2398
BssECI CCNNGG 5 cut(s) 388, 924, 1639, 1888, 2120
BssNAI GTATAC 1 cut(s) 518
BssT1I CCWWGG 4 cut(s) 388, 924, 1639, 1888
Bst1107I GTATAC 1 cut(s) 518
Bst2UI CCWGG 3 cut(s) 488, 808, 2121
Bst4CI ACNGT 8 cut(s) 284, 467, 636, 725, 952, 1071, 1678, 2104
BstAFI CTTAAG 2 cut(s) 1285, 1343
BstC8I GCNNGC 5 cut(s) 584, 1043, 1258, 2046, 2551
BstDEI CTNAG 6 cut(s) 254, 477, 535, 1273, 1301, 1769
BstENI CCTNNNNNAGG 1 cut(s) 2079
BstF5I GGATG 7 cut(s) 10, 459, 691, 1087, 1390, 1976, 2029
BstFNI CGCG 1 cut(s) 1943
BstHHI GCGC 2 cut(s) 793, 1945
BstMAI GTCTC 2 cut(s) 2283, 2415
BstMCI CGRYCG 1 cut(s) 658
BstMWI GCNNNNNNNGC 3 cut(s) 1092, 1940, 2045
BstNI CCWGG 3 cut(s) 488, 808, 2121
BstNSI RCATGY 3 cut(s) 68, 850, 2225
BstSCI CCNGG 3 cut(s) 486, 806, 2119
BstSFI CTRYAG 1 cut(s) 872
BstSLI GKGCMC 1 cut(s) 937
BstUI CGCG 1 cut(s) 1943
BstV1I GCAGC 2 cut(s) 133, 528
BstV2I GAAGAC 1 cut(s) 2404
BstX2I RGATCY 6 cut(s) 1051, 1481, 1861, 2066, 2308, 2492
BstYI RGATCY 6 cut(s) 1051, 1481, 1861, 2066, 2308, 2492
BstZ17I GTATAC 1 cut(s) 518
Bsu15I ATCGAT 2 cut(s) 210, 957
BsuI GTATCC 1 cut(s) 678
BsuRI GGCC 5 cut(s) 830, 878, 1797, 1934, 2039
BsuTUI ATCGAT 2 cut(s) 210, 957
BtsCI GGATG 7 cut(s) 10, 459, 691, 1087, 1390, 1976, 2029
BveI ACCTGC 1 cut(s) 1206
Cac8I GCNNGC 5 cut(s) 584, 1043, 1258, 2046, 2551
CfoI GCGC 2 cut(s) 793, 1945
Cfr13I GGNCC 1 cut(s) 392
ClaI ATCGAT 2 cut(s) 210, 957
CsiI ACCWGGT 1 cut(s) 806
DdeI CTNAG 6 cut(s) 254, 477, 535, 1273, 1301, 1769
DraI TTTAAA 1 cut(s) 1137
EaeI YGGCCR 2 cut(s) 828, 1932
Eco130I CCWWGG 4 cut(s) 388, 924, 1639, 1888
Eco47I GGWCC 1 cut(s) 392
Eco57I CTGAAG 1 cut(s) 747
Eco88I CYCGRG 4 cut(s) 371, 888, 938, 1244
EcoNI CCTNNNNNAGG 1 cut(s) 2079
EcoRI GAATTC 2 cut(s) 893, 1742
EcoRII CCWGG 3 cut(s) 486, 806, 2119
EcoT14I CCWWGG 4 cut(s) 388, 924, 1639, 1888
ErhI CCWWGG 4 cut(s) 388, 924, 1639, 1888
FalI AAGNNNNNCTT 4 cut(s) 1579, 1611, 1848, 1880
FauNDI CATATG 1 cut(s) 1875
FbaI TGATCA 2 cut(s) 321, 2226
FblI GTMKAC 1 cut(s) 517
Fnu4HI GCNGC 2 cut(s) 147, 542
FokI GGATG 6 cut(s) 446, 698, 1094, 1397, 1983, 2036
Fsp4HI GCNGC 2 cut(s) 147, 542
FspBI CTAG 6 cut(s) 1038, 1542, 1551, 1917, 2363, 2554
GlaI GCGC 2 cut(s) 792, 1944
GluI GCNGC 2 cut(s) 147, 542
GsuI CTGGAG 3 cut(s) 470, 1313, 2368
HaeIII GGCC 5 cut(s) 830, 878, 1797, 1934, 2039
HapII CCGG 1 cut(s) 1382
HhaI GCGC 2 cut(s) 793, 1945
Hin6I GCGC 2 cut(s) 791, 1943
HinP1I GCGC 2 cut(s) 791, 1943
HincII GTYRAC 3 cut(s) 228, 652, 721
HindII GTYRAC 3 cut(s) 228, 652, 721
HindIII AAGCTT 1 cut(s) 2042
HinfI GANTC 9 cut(s) 93, 224, 273, 817, 868, 1937, 2190, 2470, 2483
HpaI GTTAAC 1 cut(s) 721
HpaII CCGG 1 cut(s) 1382
HphI GGTGA 5 cut(s) 796, 940, 1707, 1769, 2284
Hpy166II GTNNAC 8 cut(s) 228, 415, 518, 652, 693, 721, 1777, 2098
Hpy188I TCNGA 7 cut(s) 278, 326, 603, 867, 1603, 1772, 2449
Hpy8I GTNNAC 8 cut(s) 228, 415, 518, 652, 693, 721, 1777, 2098
Hpy99I CGWCG 2 cut(s) 143, 1579
HpyCH4III ACNGT 8 cut(s) 284, 467, 636, 725, 952, 1071, 1678, 2104
HpyCH4IV ACGT 5 cut(s) 50, 138, 297, 411, 931
HpyF10VI GCNNNNNNNGC 3 cut(s) 1092, 1940, 2045
HpyF3I CTNAG 6 cut(s) 254, 477, 535, 1273, 1301, 1769
HpySE526I ACGT 5 cut(s) 50, 138, 297, 411, 931
HspAI GCGC 2 cut(s) 791, 1943
Kpn2I TCCGGA 1 cut(s) 1381
KpnI GGTACC 2 cut(s) 640, 2128
Ksp22I TGATCA 2 cut(s) 321, 2226
KspAI GTTAAC 1 cut(s) 721
Lsp1109I GCAGC 2 cut(s) 133, 528
LweI GCATC 8 cut(s) 133, 676, 1181, 1675, 1961, 2265, 2323, 2453
MabI ACCWGGT 1 cut(s) 806
MaeI CTAG 6 cut(s) 1038, 1542, 1551, 1917, 2363, 2554
MaeII ACGT 5 cut(s) 50, 138, 297, 411, 931
MaeIII GTNAC 4 cut(s) 298, 344, 442, 842
MbiI CCGCTC 1 cut(s) 192
MboII GAAGA 6 cut(s) 34, 464, 1345, 1599, 2379, 2409
MfeI CAATTG 1 cut(s) 1458
MflI RGATCY 6 cut(s) 1051, 1481, 1861, 2066, 2308, 2492
MhlI GDGCHC 1 cut(s) 937
MlyI GAGTC 6 cut(s) 233, 282, 862, 1946, 2479, 2492
MmeI TCCRAC 2 cut(s) 1783, 2001
MroI TCCGGA 1 cut(s) 1381
MroXI GAANNNNTTC 3 cut(s) 608, 1764, 2031
MslI CAYNNNNRTG 1 cut(s) 1995
MspA1I CMGCKG 1 cut(s) 422
MspCI CTTAAG 2 cut(s) 1285, 1343
MspI CCGG 1 cut(s) 1382
MspR9I CCNGG 3 cut(s) 488, 808, 2121
MunI CAATTG 1 cut(s) 1458
MvaI CCWGG 3 cut(s) 488, 808, 2121
MvnI CGCG 1 cut(s) 1943
MwoI GCNNNNNNNGC 3 cut(s) 1092, 1940, 2045
NdeI CATATG 1 cut(s) 1875
NlaIV GGNNCC 3 cut(s) 638, 1420, 2126
NmeAIII GCCGAG 2 cut(s) 285, 1960
NmuCI GTSAC 2 cut(s) 298, 442
NspI RCATGY 3 cut(s) 68, 850, 2225
PaeR7I CTCGAG 2 cut(s) 371, 938
PaqCI CACCTGC 1 cut(s) 1206
PciI ACATGT 2 cut(s) 64, 2221
PcsI WCGNNNNNNNCGW 1 cut(s) 963
PdmI GAANNNNTTC 3 cut(s) 608, 1764, 2031
PfeI GAWTC 3 cut(s) 93, 817, 2190
PkrI GCNGC 2 cut(s) 148, 543
Ple19I CGATCG 1 cut(s) 658
PleI GAGTC 6 cut(s) 232, 281, 862, 1945, 2478, 2491
PpsI GAGTC 6 cut(s) 232, 281, 862, 1945, 2478, 2491
PscI ACATGT 2 cut(s) 64, 2221
PshBI ATTAAT 1 cut(s) 528
PsiI TTATAA 1 cut(s) 554
Psp6I CCWGG 3 cut(s) 486, 806, 2119
PspGI CCWGG 3 cut(s) 486, 806, 2119
PspN4I GGNNCC 3 cut(s) 638, 1420, 2126
PspPI GGNCC 1 cut(s) 392
PspXI VCTCGAGB 2 cut(s) 371, 938
PsuI RGATCY 6 cut(s) 1051, 1481, 1861, 2066, 2308, 2492
PvuI CGATCG 1 cut(s) 658
PvuII CAGCTG 1 cut(s) 422
RseI CAYNNNNRTG 1 cut(s) 1995
SatI GCNGC 2 cut(s) 147, 542
Sau96I GGNCC 1 cut(s) 392
SchI GAGTC 6 cut(s) 233, 282, 862, 1946, 2479, 2492
ScrFI CCNGG 3 cut(s) 488, 808, 2121
SduI GDGCHC 1 cut(s) 937
SexAI ACCWGGT 1 cut(s) 806
SfaNI GCATC 8 cut(s) 133, 676, 1181, 1675, 1961, 2265, 2323, 2453
SfcI CTRYAG 1 cut(s) 872
Sfr274I CTCGAG 2 cut(s) 371, 938
SinI GGWCC 1 cut(s) 392
SlaI CTCGAG 2 cut(s) 371, 938
SmiMI CAYNNNNRTG 1 cut(s) 1995
SmlI CTYRAG 8 cut(s) 15, 371, 938, 1285, 1343, 1728, 2412, 2455
SmoI CTYRAG 8 cut(s) 15, 371, 938, 1285, 1343, 1728, 2412, 2455
SsiI CCGC 2 cut(s) 192, 1941
SspI AATATT 2 cut(s) 1366, 2141
SspMI CTAG 6 cut(s) 1038, 1542, 1551, 1917, 2363, 2554
StyD4I CCNGG 3 cut(s) 486, 806, 2119
StyI CCWWGG 4 cut(s) 388, 924, 1639, 1888
TaaI ACNGT 8 cut(s) 284, 467, 636, 725, 952, 1071, 1678, 2104
TaiI ACGT 5 cut(s) 53, 141, 300, 414, 934
TaqI TCGA 9 cut(s) 141, 210, 372, 897, 939, 957, 2063, 2146, 2424
TaqII GACCGA 1 cut(s) 2045
TatI WGTACW 1 cut(s) 1845
TfiI GAWTC 3 cut(s) 93, 817, 2190
TseFI GTSAC 2 cut(s) 298, 442
TseI GCWGC 2 cut(s) 146, 541
Tsp45I GTSAC 2 cut(s) 298, 442
TspGWI ACGGA 4 cut(s) 185, 453, 660, 1422
Vha464I CTTAAG 2 cut(s) 1285, 1343
VpaK11BI GGWCC 1 cut(s) 392
VspI ATTAAT 1 cut(s) 528
XagI CCTNNNNNAGG 1 cut(s) 2079
XapI RAATTY 8 cut(s) 749, 833, 893, 1204, 1742, 1786, 1825, 2315
XbaI TCTAGA 2 cut(s) 1541, 1550
XceI RCATGY 3 cut(s) 68, 850, 2225
XcmI CCANNNNNNNNNTGG 1 cut(s) 1019
XhoI CTCGAG 2 cut(s) 371, 938
XmiI GTMKAC 1 cut(s) 517
XmnI GAANNNNTTC 3 cut(s) 608, 1764, 2031
XspI CTAG 6 cut(s) 1038, 1542, 1551, 1917, 2363, 2554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.