MD17G1266000.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000083
Physical Location & Seq
Reverse (-)
32654744 .. 32658912
4169 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1266000.v1.1.491

Sequence Viewer

Length: 2655 bp
ATGGGAATGCTGAAAAACCTTCGATTTGTATTCCTTTGTTGTTTTTCTCTTATACTTCTAGTTCATGCACAAGATGATCAATCAGGTTTCATCAGCTTGGACTGTGGACGTCCATCAAATTCTAACAACTCCGAGTCACCAACCGGCGTCGACTACATTTCAGATGCAGCCTTCATAAGCACTGGTGTCAGCAAAAGCGTCGCACCTGCATACAAAGCTACTTATCCGCTGCAGGCAGCTTATGTCAGGAGCTTTCCTCAGGGGGTCAAGAACTGTTACAAAGTAAACATCACAAAGAACACCAAGTATTTAATCCGAGCGGTTTTCCTGTATGGTAATTATGACGGCCTAAATAAGCTGCCGAAGTTTGATCTTGCCATCGGAGCTAGTTACTGGGATTCAGTGGCATTTACTGATGATGCATCTTTAAGTACGTTCAATGAAGTTGTTCACATTCCCACGTTAGACTACATAAATGTGTGCTTACTCAACGAGGGAACCGGAACACCATTCATTTCATCACTGGAATTAAGGCCTTTGAAAAACACTACTTATGTGACTCCAACGGGTTCCTTGGCACTCTTCTTTCGATATGATGTCGGTTCAACAGTTGATGATCAAACATACAGGTATCCCGATGATGCTTTGGATCGGTTTTGGGAACCCTTCAACTTCAATAAGTGGACGCAGTTAGATACCCCGTTTACCGTTGATGGTCAAAACAATGATTACCAAGTACCATCTATCGTAATGAGATCTGCCAGCACGCCTCTATATGATACGATGGGCTTCGATTGGAATGCCCCGGATCCAAGTACACAATACTATATCTTCAGTCACTTTGCTGAACTCCAGCTGCTTGAAGCCGACCAATTTAGATCATTCGATATCACGCTGAATGGAGATTATTTGTATGGACCTGTTGTCCCGGCTTACTTGTCCACAACCACTGTGCTTAGCAACCCATCCTTAACCGGAGGCAATTACAGTTTCTTACTTGTTCAAACGGAGGATTCAAATCTCCCACCAATTCTCAATGCCATTGAGGTCTACAGATTGATAGATTTATCACAACCTGAAACCGACACAGATGATGTGTCTGCCATCACAAACGTAAAGTCAACATATGGAGTGGACAAAGATTGGCAAGGAGACCCATGTACTCCTGAAGGCTACATGTGGGAAGGTCTAAATTGTACCTTCAACGGTTCCCCCAGAATTATATCCTTGGACCTGTCCTCGAGTGGATTGACCGGGGAGATAACTTCATATATATCTGACCTCGCCGTGTTACAATCTTTGGATTTATCACACAACAGCTTAAAGGGATCAATACCTGAATTTCTGTCTAGAATGCGAAATCTGAAAGTCCTCAACCTAGAAGGAAACAAGCTCAACGGTTCAGTTCCAGCCGACCTCATTGAAAGATCGAAAAGTGGTTCTCTATCATTGAGTGTTGGAGAAAATGCAGACCTATGTGTAGAAAATTCATGCAAAAAGGAGGAGACAAAGAAAAAGAAGAAGAAGAACATAATTGTTCCAATCGTAGCAGCAATTGGTGGATTTTTTATTCTTGTGGTGGTACTTGTAGTGGCTATATTCATGTGGCTAAAAAGAGGAAGGAAGCAAGAAGTTGTAACAGCAGTTTCACATCAACCCAACAATCAAGTTGATTCATTCGAGTCCAAAAAACGACAGTTTACATACTCGGACGTCCTGAAGATTACAAACAACTTTCAGACAATTATTGGCAGAGGTGGATTCGGAAACGTTTACCATGGTTTTGTCGATGATACTCAAGTAGCTGTGAAGATGCTTTCTCCAACATCAGGTCAAGGATACCAACAGTTTCAAGCAGAGGTCAAACTTCTAATCAGAGTTCATCACAGAAACTTGACTAGTCTTGTGGGATACTGCAACGAAGGAACCAACATGGCGCTCATCTATGAATTTATGGCCTACGGAAGTTTGGACTCGCATCTTCTAGGTGAGAATAGTAATGCAAATGTTTTGACTTGGGAAGGTAGACTCCAAATAGCAACAGATGCAGCACAAGGATTGGAGTACCTTCACAATGGTTGCAAGCCACCCATAGTCCACAGAGATGTGAAGACAACAAATATTTTGCTAGCCGAAAATTTCCAAGCAAAATTAGCTGATTTTGGTCTGTCCAGAATTTTCCCAACCGATGGAGGCACTCATATGTCTACTGCTGTAGCTGGAACCCCGGGATACCTTGATCCCGAGTACCACAAAACAGGTTGGCTCAATGAGAAGAGCGATGTTTATAGCTTTGGAGTTGTTCTGTTGGAGATCATCACTGGCCGCCCTGCGATATCAAGAACACATGAGAAGATTCCTGTAAGTCAATGGGTTAGCTCCATGCTTGCTAGAGGGGATATCAAAACTATTCTAGATGCGAGATTACTTGGAGATTACGATATCAACTCTGCCTGGAAGGCTGTTGAACTAGCAATGAATTGTGTGTCGCATACATCCAACAAAAGGCCAAACATGAGTGAGGTAGTGAGAGGGCTTAAGGATTGTTTGGAAGCTGAGTTGGCTCGAACGAACTTCAACCGTGTGACTGAATCAACGGATTCAGTTATTTGCTCTATGAATGTGAATACGGAATTTAGTCCTTTGGTAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

885

Amino Acids

98.04

Weight (kDa)

5.31

Isoelectric Point (pI)

31.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 33 - 352 1.8e-108 Malectin-like domain
LRR_8 PF13855 409 - 465 8.5e-08 Leucine rich repeat
LRR_4 PF12799 431 - 465 3.4e-06 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 579 - 845 1.1e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 580 - 843 3.5e-40 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000083)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07560 AT1G51790 AT1G51790 AT1G51790 AT1G51800 AT1G51805 AT1G51805 AT1G51810 AT1G51820 AT1G51820 AT1G51830 AT1G51830 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51850 AT1G51850 AT1G51860 AT1G51860 AT1G51870 AT1G51870 AT1G51880 AT1G51880 AT1G51880 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51910 AT2G04300 AT2G04300 AT2G28960 AT2G28960 AT2G28970 AT2G28970 AT2G28990 AT2G28990 AT2G29000 AT3G21340 AT3G46350 AT5G59616 AT5G59650 AT5G59650 AT5G59660 AT5G59660 AT5G59660 AT5G59670 AT5G59670 AT5G59680
fragaria_vesca FvH4_2g03270 FvH4_3g21300 FvH4_6g32660 FvH4_6g32660 FvH4_6g32670 FvH4_6g32670 FvH4_6g32690 FvH4_6g32690 FvH4_6g32691 FvH4_6g32700 FvH4_6g32740 FvH4_6g32750 FvH4_6g33210 FvH4_6g33210 FvH4_7g24270 FvH4_7g24280 FvH4_7g24291 FvH4_7g24300
malus_domestica MD09G1198400.v1.1 MD09G1198600.v1.1 MD09G1199100.v1.1 MD09G1199200.v1.1 MD09G1199300.v1.1 MD09G1199500.v1.1 MD09G1199600.v1.1 MD17G1179300.v1.1 MD17G1180200.v1.1 MD17G1266000.v1.1
prunus_persica Prupe.1G438900_v2.0.a1 Prupe.1G439000_v2.0.a1 Prupe.1G439100_v2.0.a1 Prupe.3G019400_v2.0.a1 Prupe.3G021900_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G055100_v2.0.a1 Prupe.3G055400_v2.0.a1 Prupe.3G055500_v2.0.a1 Prupe.3G055600_v2.0.a1 Prupe.3G055700_v2.0.a1 Prupe.3G055800_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056100_v2.0.a1 Prupe.3G209600_v2.0.a1 Prupe.3G209700_v2.0.a1 Prupe.6G291100_v2.0.a1 Prupe.7G208900_v2.0.a1 Prupe.8G038300_v2.0.a1
pyrus_communis pycom09g11570 pycom09g11580 pycom09g11610 pycom09g11630 pycom09g11640 pycom17g18840 pycom17g18910 pycom17g18930 pycom17g18940
rosa_chinensis RchiOBHm_Chr1g0317521 RchiOBHm_Chr1g0317541 RchiOBHm_Chr2g0123351 RchiOBHm_Chr2g0141181 RchiOBHm_Chr2g0141191 RchiOBHm_Chr2g0141211 RchiOBHm_Chr2g0141221 RchiOBHm_Chr2g0141261 RchiOBHm_Chr2g0141271 RchiOBHm_Chr2g0141311 RchiOBHm_Chr2g0141331 RchiOBHm_Chr2g0141371 RchiOBHm_Chr2g0141391 RchiOBHm_Chr2g0141401 RchiOBHm_Chr2g0141431 RchiOBHm_Chr2g0141481 RchiOBHm_Chr2g0141491 RchiOBHm_Chr2g0141591 RchiOBHm_Chr2g0141601 RchiOBHm_Chr2g0141611 RchiOBHm_Chr2g0141631 RchiOBHm_Chr2g0141641 RchiOBHm_Chr2g0141661 RchiOBHm_Chr2g0141681 RchiOBHm_Chr2g0141701 RchiOBHm_Chr2g0141781 RchiOBHm_Chr2g0141811 RchiOBHm_Chr2g0141821 RchiOBHm_Chr3g0479311 RchiOBHm_Chr3g0495751 RchiOBHm_Chr4g0404741 RchiOBHm_Chr4g0410341 RchiOBHm_Chr4g0418371 RchiOBHm_Chr4g0438651 RchiOBHm_Chr5g0036411 RchiOBHm_Chr5g0036491 RchiOBHm_Chr5g0042391 RchiOBHm_Chr6g0279871 RchiOBHm_Chr7g0192921 RchiOBHm_Chr7g0214901 RchiOBHm_Chr7g0234931
rosa_laevigata RLG00000003432 RLG00000008806 RLG00000013062 RLG00000019514 RLG00000019923 RLG00000019924 RLG00000019925 RLG00000019930 RLG00000019931 RLG00000019934 RLG00000019937 RLG00000019938 RLG00000033697
rosa_multiflora Rmu_co8002302.1_g000001 Rmu_sc0000147.1_g000031 Rmu_sc0000463.1_g000005 Rmu_sc0000712.1_g000003 Rmu_sc0001483.1_g000017 Rmu_sc0001554.1_g000002 Rmu_sc0001554.1_g000005 Rmu_sc0001554.1_g000008 Rmu_sc0001981.1_g000017 Rmu_sc0002040.1_g000056 Rmu_sc0002755.1_g000018 Rmu_sc0002804.1_g000016 Rmu_sc0002928.1_g000015 Rmu_sc0003040.1_g000008 Rmu_sc0003040.1_g000018 Rmu_sc0004512.1_g000001 Rmu_sc0004512.1_g000004 Rmu_sc0005507.1_g000001 Rmu_sc0005507.1_g000009 Rmu_sc0005507.1_g000033 Rmu_sc0005507.1_g000037 Rmu_sc0005507.1_g000043 Rmu_sc0005507.1_g000047 Rmu_sc0006318.1_g000004 Rmu_sc0006318.1_g000014 Rmu_sc0006318.1_g000021 Rmu_sc0006556.1_g000001 Rmu_sc0007208.1_g000002 Rmu_sc0007208.1_g000003 Rmu_sc0007208.1_g000006 Rmu_sc0007208.1_g000008
rosa_roxburghii Rroxscaffold_1G00044430 Rroxscaffold_2G00103420 Rroxscaffold_2G00103470 Rroxscaffold_2G00103490 Rroxscaffold_2G00103500 Rroxscaffold_2G00103520 Rroxscaffold_2G00103530 Rroxscaffold_2G00103540 Rroxscaffold_2G00103550 Rroxscaffold_2G00103570 Rroxscaffold_2G00103770 Rroxscaffold_2G00103780 Rroxscaffold_2G00103800 Rroxscaffold_2G00103820 Rroxscaffold_2G00103860 Rroxscaffold_2G00103890 Rroxscaffold_2G00103930 Rroxscaffold_2G00103940 Rroxscaffold_2G00104010 Rroxscaffold_2G00104030 Rroxscaffold_2G00104050 Rroxscaffold_2G00104060 Rroxscaffold_2G00104070 Rroxscaffold_2G00104090 Rroxscaffold_2G00104100 Rroxscaffold_2G00104120 Rroxscaffold_2G00104130 Rroxscaffold_4G00331710
rosa_rugosa Rorug02G0367500 Rorug02G0367500 Rorug02G0367600 Rorug02G0367800 Rorug02G0367800 Rorug02G0367900 Rorug02G0368100 Rorug02G0368100 Rorug02G0368100 Rorug02G0368200 Rorug02G0368700 Rorug02G0368900 Rorug05G0155600 Rorug05G0155700 Rorug05G0155800 Rorug05G0156700 Rorug05G0157300
rosa_samantha Rh1AG028000 Rh2AG418700 Rh2AG418900 Rh2AG419000 Rh2AG419100 Rh2AG419400 Rh2AG419600 Rh2AG419800 Rh2AG420400 Rh2AG421000 Rh2BG428500 Rh2BG428600 Rh2BG428800 Rh2BG428900 Rh2BG429300 Rh2BG429400 Rh2BG429700 Rh2BG429900 Rh2BG430000 Rh2BG430700 Rh2CG405100 Rh2CG405200 Rh2CG405300 Rh2CG405500 Rh2CG405600 Rh2CG405700 Rh2CG406000 Rh2CG406100 Rh2CG406300 Rh2CG406600 Rh2CG406700 Rh2CG407500 Rh2CG408100 Rh2DG438500 Rh3DG123900 Rh3DG124000 Rh5BG249700 Rh5BG482500 Rh5CG281500 Rh5CG507300 Rh7DG133300
rosa_wichuraiana Rw0G019720 Rw0G019730 Rw0G019750 Rw0G023010 Rw2G034340 Rw2G034350 Rw2G034370 Rw2G034380 Rw2G034400 Rw2G034430 Rw2G034470 Rw2G034480 Rw2G034510 Rw5G022890 Rw5G026410 Rw5G032710 Rw5G042850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 214
AatII GACGTC 2 cut(s) 112, 1716
Acc36I ACCTGC 1 cut(s) 214
AccB7I CCANNNNNTGG 1 cut(s) 2189
AccBSI CCGCTC 1 cut(s) 320
AccI GTMKAC 4 cut(s) 150, 1050, 2026, 2207
AciI CCGC 3 cut(s) 227, 320, 2326
AclI AACGTT 1 cut(s) 1770
AclWI GGATC 5 cut(s) 657, 803, 816, 1336, 2234
AcoI YGGCCR 1 cut(s) 2323
AcsI RAATTY 7 cut(s) 118, 1340, 1486, 1949, 2137, 2175, 2633
AcuI CTGAAG 3 cut(s) 817, 1188, 1739
AcyI GRCGYC 3 cut(s) 109, 147, 1713
AfaI GTAC 8 cut(s) 433, 738, 817, 1162, 1198, 1584, 2066, 2249
AfiI CCNNNNNNNGG 3 cut(s) 1829, 2189, 2505
AflII CTTAAG 1 cut(s) 2537
AflIII ACRYGT 1 cut(s) 1176
AhdI GACNNNNNGTC 1 cut(s) 923
AhlI ACTAGT 1 cut(s) 1898
AjnI CCWGG 1 cut(s) 2453
Alw26I GTCTC 2 cut(s) 1146, 1499
AlwI GGATC 5 cut(s) 657, 803, 816, 1336, 2234
Ama87I CYCGRG 3 cut(s) 1240, 2227, 2243
AoxI GGCC 5 cut(s) 346, 533, 1956, 2323, 2507
ApeKI GCWGC 7 cut(s) 167, 229, 236, 358, 856, 1550, 2048
ApoI RAATTY 7 cut(s) 118, 1340, 1486, 1949, 2137, 2175, 2633
ArsI GACNNNNNNTTYG 2 cut(s) 1103, 1135
Asp700I GAANNNNTTC 1 cut(s) 447
AspLEI GCGC 1 cut(s) 1939
AspS9I GGNCC 2 cut(s) 917, 1231
AsuC2I CCSGG 5 cut(s) 806, 929, 1255, 2228, 2229
AsuHPI GGTGA 2 cut(s) 129, 2000
AsuNHI GCTAGC 1 cut(s) 2128
AvaI CYCGRG 3 cut(s) 1240, 2227, 2243
AvaII GGWCC 2 cut(s) 917, 1231
AxyI CCTNAGG 1 cut(s) 258
BaeI ACNNNNGTAYC 2 cut(s) 1785, 1818
BamHI GGATCC 1 cut(s) 808
BbsI GAAGAC 1 cut(s) 2117
BbvI GCAGC 7 cut(s) 179, 216, 248, 345, 843, 1562, 2060
BccI CCATC 8 cut(s) 121, 386, 707, 748, 778, 973, 1112, 2183
BceAI ACGGC 2 cut(s) 361, 1271
BcgI CGANNNNNNTGC 4 cut(s) 181, 215, 782, 816
BciT130I CCWGG 1 cut(s) 2455
BciVI GTATCC 4 cut(s) 642, 1832, 1904, 2225
BclI TGATCA 2 cut(s) 76, 616
BcnI CCSGG 5 cut(s) 806, 929, 1255, 2228, 2229
BcoDI GTCTC 2 cut(s) 1146, 1499
BcuI ACTAGT 1 cut(s) 1898
BfmI CTRYAG 3 cut(s) 230, 1051, 2214
BfoI RGCGCY 1 cut(s) 1940
BfrI CTTAAG 1 cut(s) 2537
BfuAI ACCTGC 1 cut(s) 214
BfuI GTATCC 4 cut(s) 642, 1832, 1904, 2225
BglI GCCNNNNNGGC 1 cut(s) 2459
BglII AGATCT 1 cut(s) 755
BisI GCNGC 8 cut(s) 168, 230, 237, 359, 857, 1551, 2049, 2326
BlpI GCTNAGC 1 cut(s) 956
BlsI GCNGC 8 cut(s) 169, 231, 238, 360, 858, 1552, 2050, 2327
Bme1390I CCNGG 6 cut(s) 806, 929, 1255, 2228, 2229, 2455
Bme18I GGWCC 2 cut(s) 917, 1231
BmeRI GACNNNNNGTC 1 cut(s) 923
BmeT110I CYCGRG 3 cut(s) 1240, 2227, 2243
BmgT120I GGNCC 2 cut(s) 917, 1231
BmiI GGNNCC 7 cut(s) 499, 571, 663, 810, 1210, 1927, 2224
BmrFI CCNGG 6 cut(s) 806, 929, 1255, 2228, 2229, 2455
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 8 cut(s) 154, 409, 431, 631, 1803, 1987, 2035, 2407
BmtI GCTAGC 1 cut(s) 2132
BmuI ACTGGG 1 cut(s) 403
BpiI GAAGAC 1 cut(s) 2117
BplI GAGNNNNNCTC 2 cut(s) 241, 273
BpmI CTGGAG 1 cut(s) 836
Bpu1102I GCTNAGC 1 cut(s) 956
BpuEI CTTGAG 1 cut(s) 1782
BpuMI CCSGG 5 cut(s) 806, 929, 1255, 2228, 2229
BsaBI GATNNNNATC 1 cut(s) 1017
BsaHI GRCGYC 3 cut(s) 109, 147, 1713
BsaI GGTCTC 1 cut(s) 1146
BsaJI CCNNGG 7 cut(s) 573, 804, 1227, 1254, 1777, 2226, 2227
BsaWI WCCGGW 2 cut(s) 500, 974
Bsc4I CCNNNNNNNGG 3 cut(s) 1829, 2189, 2505
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 4 cut(s) 187, 398, 528, 2326
Bse21I CCTNAGG 1 cut(s) 258
Bse3DI GCAATG 1 cut(s) 2481
Bse8I GATNNNNATC 1 cut(s) 1017
BseBI CCWGG 1 cut(s) 2455
BseDI CCNNGG 7 cut(s) 573, 804, 1227, 1254, 1777, 2226, 2227
BseGI GGATG 2 cut(s) 965, 2495
BseJI GATNNNNATC 1 cut(s) 1017
BseLI CCNNNNNNNGG 3 cut(s) 1829, 2189, 2505
BseMI GCAATG 1 cut(s) 2481
BseMII CTCAG 2 cut(s) 272, 2547
BseNI ACTGG 4 cut(s) 187, 398, 528, 2326
BseRI GAGGAG 1 cut(s) 1517
BseXI GCAGC 7 cut(s) 179, 216, 248, 345, 843, 1562, 2060
BshFI GGCC 5 cut(s) 348, 535, 1958, 2325, 2509
BsiHKCI CYCGRG 3 cut(s) 1240, 2227, 2243
BsiSI CCGG 7 cut(s) 144, 501, 806, 929, 975, 1254, 2228
BslFI GGGAC 1 cut(s) 911
BslI CCNNNNNNNGG 3 cut(s) 1829, 2189, 2505
BsmAI GTCTC 2 cut(s) 1146, 1499
BsmFI GGGAC 1 cut(s) 911
BsmI GAATGC 3 cut(s) 12, 805, 1359
BsnI GGCC 5 cut(s) 348, 535, 1958, 2325, 2509
Bso31I GGTCTC 1 cut(s) 1146
BsoBI CYCGRG 3 cut(s) 1240, 2227, 2243
Bsp1720I GCTNAGC 1 cut(s) 956
Bsp19I CCATGG 1 cut(s) 1777
BspACI CCGC 3 cut(s) 227, 320, 2326
BspANI GGCC 5 cut(s) 348, 535, 1958, 2325, 2509
BspCNI CTCAG 2 cut(s) 271, 2548
BspLI GGNNCC 7 cut(s) 499, 571, 663, 810, 1210, 1927, 2224
BspMAI CTGCAG 1 cut(s) 234
BspMI ACCTGC 1 cut(s) 214
BspOI GCTAGC 1 cut(s) 2132
BspPI GGATC 5 cut(s) 657, 803, 816, 1336, 2234
BspQI GCTCTTC 1 cut(s) 2270
BspTI CTTAAG 1 cut(s) 2537
BspTNI GGTCTC 1 cut(s) 1146
BsrBI CCGCTC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 2481
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 4 cut(s) 187, 398, 528, 2326
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 7 cut(s) 573, 804, 1227, 1254, 1777, 2226, 2227
BssNI GRCGYC 3 cut(s) 109, 147, 1713
BssT1I CCWWGG 3 cut(s) 573, 1227, 1777
Bst2UI CCWGG 1 cut(s) 2455
Bst6I CTCTTC 2 cut(s) 587, 2270
BstACI GRCGYC 3 cut(s) 109, 147, 1713
BstAFI CTTAAG 1 cut(s) 2537
BstAPI GCANNNNNTGC 1 cut(s) 2045
BstC8I GCNNGC 6 cut(s) 234, 763, 767, 2084, 2130, 2388
BstDEI CTNAG 3 cut(s) 258, 956, 2556
BstDSI CCRYGG 1 cut(s) 1777
BstF5I GGATG 2 cut(s) 965, 2495
BstH2I RGCGCY 1 cut(s) 1940
BstHHI GCGC 1 cut(s) 1939
BstMAI GTCTC 2 cut(s) 1146, 1499
BstMWI GCNNNNNNNGC 6 cut(s) 215, 383, 2045, 2153, 2459, 2561
BstNI CCWGG 1 cut(s) 2455
BstNSI RCATGY 1 cut(s) 1180
BstSCI CCNGG 6 cut(s) 804, 927, 1253, 2226, 2227, 2453
BstSFI CTRYAG 3 cut(s) 230, 1051, 2214
BstV1I GCAGC 7 cut(s) 179, 216, 248, 345, 843, 1562, 2060
BstV2I GAAGAC 1 cut(s) 2117
BstX2I RGATCY 2 cut(s) 755, 808
BstYI RGATCY 2 cut(s) 755, 808
Bsu36I CCTNAGG 1 cut(s) 258
BsuI GTATCC 4 cut(s) 642, 1832, 1904, 2225
BsuRI GGCC 5 cut(s) 348, 535, 1958, 2325, 2509
BtgI CCRYGG 1 cut(s) 1777
BtgZI GCGATG 1 cut(s) 2295
BtsCI GGATG 2 cut(s) 965, 2495
BtsIMutI CAGTG 5 cut(s) 180, 408, 521, 948, 2319
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 6 cut(s) 234, 763, 767, 2084, 2130, 2388
CfoI GCGC 1 cut(s) 1939
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 2 cut(s) 917, 1231
Cfr9I CCCGGG 1 cut(s) 2227
CseI GACGC 3 cut(s) 136, 187, 694
Csp6I GTAC 8 cut(s) 432, 737, 816, 1161, 1197, 1583, 2065, 2248
CviQI GTAC 8 cut(s) 432, 737, 816, 1161, 1197, 1583, 2065, 2248
DdeI CTNAG 3 cut(s) 258, 956, 2556
DriI GACNNNNNGTC 1 cut(s) 923
EaeI YGGCCR 1 cut(s) 2323
Eam1104I CTCTTC 2 cut(s) 587, 2270
Eam1105I GACNNNNNGTC 1 cut(s) 923
EarI CTCTTC 2 cut(s) 587, 2270
Eco130I CCWWGG 3 cut(s) 573, 1227, 1777
Eco147I AGGCCT 1 cut(s) 535
Eco31I GGTCTC 1 cut(s) 1146
Eco32I GATATC 4 cut(s) 889, 2337, 2401, 2443
Eco47I GGWCC 2 cut(s) 917, 1231
Eco57I CTGAAG 3 cut(s) 817, 1188, 1739
Eco81I CCTNAGG 1 cut(s) 258
Eco88I CYCGRG 3 cut(s) 1240, 2227, 2243
EcoRII CCWGG 1 cut(s) 2453
EcoRV GATATC 4 cut(s) 889, 2337, 2401, 2443
EcoT14I CCWWGG 3 cut(s) 573, 1227, 1777
EcoT22I ATGCAT 1 cut(s) 424
ErhI CCWWGG 3 cut(s) 573, 1227, 1777
FaqI GGGAC 1 cut(s) 911
FauNDI CATATG 2 cut(s) 1126, 2202
FbaI TGATCA 2 cut(s) 76, 616
FblI GTMKAC 4 cut(s) 150, 1050, 2026, 2207
Fnu4HI GCNGC 8 cut(s) 168, 230, 237, 359, 857, 1551, 2049, 2326
FokI GGATG 2 cut(s) 952, 2482
Fsp4HI GCNGC 8 cut(s) 168, 230, 237, 359, 857, 1551, 2049, 2326
GlaI GCGC 1 cut(s) 1938
GluI GCNGC 8 cut(s) 168, 230, 237, 359, 857, 1551, 2049, 2326
GsuI CTGGAG 1 cut(s) 836
HaeII RGCGCY 1 cut(s) 1940
HaeIII GGCC 5 cut(s) 348, 535, 1958, 2325, 2509
HapII CCGG 7 cut(s) 144, 501, 806, 929, 975, 1254, 2228
HgaI GACGC 3 cut(s) 136, 187, 694
HhaI GCGC 1 cut(s) 1939
Hin1I GRCGYC 3 cut(s) 109, 147, 1713
Hin6I GCGC 1 cut(s) 1937
HinP1I GCGC 1 cut(s) 1937
HincII GTYRAC 2 cut(s) 151, 1122
HindII GTYRAC 2 cut(s) 151, 1122
HpaII CCGG 7 cut(s) 144, 501, 806, 929, 975, 1254, 2228
HphI GGTGA 2 cut(s) 129, 2000
Hpy99I CGWCG 2 cut(s) 152, 203
HpyCH4IV ACGT 6 cut(s) 109, 434, 461, 1113, 1713, 1770
HpyF10VI GCNNNNNNNGC 6 cut(s) 215, 383, 2045, 2153, 2459, 2561
HpyF3I CTNAG 3 cut(s) 258, 956, 2556
HpySE526I ACGT 6 cut(s) 109, 434, 461, 1113, 1713, 1770
Hsp92I GRCGYC 3 cut(s) 109, 147, 1713
HspAI GCGC 1 cut(s) 1937
Ksp22I TGATCA 2 cut(s) 76, 616
LguI GCTCTTC 1 cut(s) 2270
LmnI GCTCC 3 cut(s) 249, 383, 2384
Lsp1109I GCAGC 7 cut(s) 179, 216, 248, 345, 843, 1562, 2060
LweI GCATC 8 cut(s) 154, 409, 431, 631, 1803, 1987, 2035, 2407
MaeII ACGT 6 cut(s) 109, 434, 461, 1113, 1713, 1770
MaeIII GTNAC 8 cut(s) 135, 275, 389, 556, 836, 1290, 1636, 2584
MbiI CCGCTC 1 cut(s) 320
MfeI CAATTG 1 cut(s) 1554
MflI RGATCY 2 cut(s) 755, 808
MlyI GAGTC 5 cut(s) 143, 553, 1691, 1967, 2022
MmeI TCCRAC 5 cut(s) 587, 1438, 1847, 2289, 2523
Mph1103I ATGCAT 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 6 cut(s) 311, 428, 530, 971, 1322, 2538
MslI CAYNNNNRTG 3 cut(s) 476, 2103, 2201
MspA1I CMGCKG 2 cut(s) 229, 856
MspCI CTTAAG 1 cut(s) 2537
MspI CCGG 7 cut(s) 144, 501, 806, 929, 975, 1254, 2228
MspR9I CCNGG 6 cut(s) 806, 929, 1255, 2228, 2229, 2455
MunI CAATTG 1 cut(s) 1554
Mva1269I GAATGC 3 cut(s) 12, 805, 1359
MvaI CCWGG 1 cut(s) 2455
MwoI GCNNNNNNNGC 6 cut(s) 215, 383, 2045, 2153, 2459, 2561
NciI CCSGG 5 cut(s) 806, 929, 1255, 2228, 2229
NcoI CCATGG 1 cut(s) 1777
NdeI CATATG 2 cut(s) 1126, 2202
NheI GCTAGC 1 cut(s) 2128
NlaIV GGNNCC 7 cut(s) 499, 571, 663, 810, 1210, 1927, 2224
NmuCI GTSAC 4 cut(s) 135, 556, 836, 2584
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 1 cut(s) 1180
PaeR7I CTCGAG 1 cut(s) 1240
PaqCI CACCTGC 1 cut(s) 214
PceI AGGCCT 1 cut(s) 535
PciI ACATGT 1 cut(s) 1176
PciSI GCTCTTC 1 cut(s) 2270
PctI GAATGC 3 cut(s) 12, 805, 1359
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 7 cut(s) 398, 1013, 1673, 1761, 2356, 2591, 2600
PflMI CCANNNNNTGG 1 cut(s) 2189
PkrI GCNGC 8 cut(s) 169, 231, 238, 360, 858, 1552, 2050, 2327
PleI GAGTC 5 cut(s) 142, 553, 1690, 1967, 2022
PpsI GAGTC 5 cut(s) 142, 553, 1690, 1967, 2022
PscI ACATGT 1 cut(s) 1176
Psp1406I AACGTT 1 cut(s) 1770
Psp6I CCWGG 1 cut(s) 2453
PspGI CCWGG 1 cut(s) 2453
PspN4I GGNNCC 7 cut(s) 499, 571, 663, 810, 1210, 1927, 2224
PspPI GGNCC 2 cut(s) 917, 1231
PspXI VCTCGAGB 1 cut(s) 1240
PstI CTGCAG 1 cut(s) 234
PsuI RGATCY 2 cut(s) 755, 808
PvuII CAGCTG 1 cut(s) 856
RsaI GTAC 8 cut(s) 433, 738, 817, 1162, 1198, 1584, 2066, 2249
RsaNI GTAC 8 cut(s) 432, 737, 816, 1161, 1197, 1583, 2065, 2248
RseI CAYNNNNRTG 3 cut(s) 476, 2103, 2201
SalI GTCGAC 1 cut(s) 149
SapI GCTCTTC 1 cut(s) 2270
SaqAI TTAA 6 cut(s) 311, 428, 530, 971, 1322, 2538
SatI GCNGC 8 cut(s) 168, 230, 237, 359, 857, 1551, 2049, 2326
Sau96I GGNCC 2 cut(s) 917, 1231
SchI GAGTC 5 cut(s) 143, 553, 1691, 1967, 2022
ScrFI CCNGG 6 cut(s) 806, 929, 1255, 2228, 2229, 2455
SfaNI GCATC 8 cut(s) 154, 409, 431, 631, 1803, 1987, 2035, 2407
SfcI CTRYAG 3 cut(s) 230, 1051, 2214
Sfr274I CTCGAG 1 cut(s) 1240
SinI GGWCC 2 cut(s) 917, 1231
SlaI CTCGAG 1 cut(s) 1240
SmaI CCCGGG 1 cut(s) 2229
SmiMI CAYNNNNRTG 3 cut(s) 476, 2103, 2201
SmlI CTYRAG 3 cut(s) 1240, 1797, 2537
SmoI CTYRAG 3 cut(s) 1240, 1797, 2537
SpeI ACTAGT 1 cut(s) 1898
SseBI AGGCCT 1 cut(s) 535
SsiI CCGC 3 cut(s) 227, 320, 2326
SspI AATATT 1 cut(s) 2122
StuI AGGCCT 1 cut(s) 535
StyD4I CCNGG 6 cut(s) 804, 927, 1253, 2226, 2227, 2453
StyI CCWWGG 3 cut(s) 573, 1227, 1777
TaiI ACGT 6 cut(s) 112, 437, 464, 1116, 1716, 1773
TatI WGTACW 2 cut(s) 815, 1160
TauI GCSGC 1 cut(s) 2328
TfiI GAWTC 7 cut(s) 398, 1013, 1673, 1761, 2356, 2591, 2600
Tru1I TTAA 6 cut(s) 311, 428, 530, 971, 1322, 2538
Tru9I TTAA 6 cut(s) 311, 428, 530, 971, 1322, 2538
TscAI CASTG 5 cut(s) 187, 408, 528, 955, 2326
TseFI GTSAC 4 cut(s) 135, 556, 836, 2584
TseI GCWGC 7 cut(s) 167, 229, 236, 358, 856, 1550, 2048
Tsp45I GTSAC 4 cut(s) 135, 556, 836, 2584
TspGWI ACGGA 4 cut(s) 1022, 1977, 2612, 2645
TspMI CCCGGG 1 cut(s) 2227
TspRI CASTG 5 cut(s) 187, 408, 528, 955, 2326
Van91I CCANNNNNTGG 1 cut(s) 2189
Vha464I CTTAAG 1 cut(s) 2537
VpaK11BI GGWCC 2 cut(s) 917, 1231
XapI RAATTY 7 cut(s) 118, 1340, 1486, 1949, 2137, 2175, 2633
XbaI TCTAGA 2 cut(s) 1349, 2413
XceI RCATGY 1 cut(s) 1180
XhoI CTCGAG 1 cut(s) 1240
XmaI CCCGGG 1 cut(s) 2227
XmiI GTMKAC 4 cut(s) 150, 1050, 2026, 2207
XmnI GAANNNNTTC 1 cut(s) 447
ZraI GACGTC 2 cut(s) 110, 1714
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.