Rorug02G0368200

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000083
Physical Location & Seq
Reverse (-)
46951562 .. 46955386
3825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0368200.1

Sequence Viewer

Length: 3276 bp
ATGGCTGAAGCTCTCGTTTCCAACCTATTAGCAAAGCTGGCTTTCATCTCTTTTACACAGATGGAACAAGAGGTGAGACTGATCGTGGGCGTTGACAAAGAAGTTAAAAATCTGACCCTCCATCTCGAAGCACTTCAAGCTGTGCTCCTGAATGCAGAGGAGAGACAAATCAAGGAGCCGGATGTCCGGAGTTGGCTGAACAACCTCAAAGACGTGTCCTTTGACATAACCGACGTATTGGACGAGTGGAACACAGAGATTCTCAAACACCGAATTGAGAAGCAAGAAAAGCATGGTGAGGAAGCTGTAGTTGCTCAAAGGAAGGTACGTTTCTTCATTTCATGTCCTAGCTGTCCCTGCAATTGTTTTGGTCAAGTTGGTAGTGTAAGTGTGCGTCATGACATTGCTAAGAAGATAAAAGAAATGAATGAAAGATTAACTTTGATAAGTAGTATAAGACAGAATTATCGTTTTGAGAGAACAATAAGAGGCAATGAACAACTTGAACATCCCAAAACGTCTTCTTTTGTTGATGTATCTAGAATAATTGGTAGAGAAGATGAAAAAAATAATTTAGTTAGCAAATTATTACCAAGCCAGAGTAGTCAAGAAGGGAGGAGTCATCCTGTCGTCCTTATTGTAGGGATGGGAGGAATGGGCAAGACTGCTCTTGCCCAATTGATCTATAACAATGAAAACATTAAAACTCATTTTGATAAGAGAATTTGGGTTTGTGTGTCTGATCCTTTTGAAGAAATTAAGATTGCCAAAGCCATCATTGAGGCTCTCAATATATACGACAGCCGAGTTAATTCAAATGAGTTACAGACTTTATTGCAGTGTGTATCTGAATGTATTGGGCATAAGAGGTTTCTTCTTGTTTTAGATGATGTGTGGAACCTAAACAATACTAAGTGGGAACAATTAAAGGAAGCTTTACAGAATGGTGCTTCCGGTAGTAGAATTTTAGTGACCACACGTAAAGAAATAGTCTCAATTACAATGCAAGTAACTACCGATCATGTGATCTATCTGAACAAGTTGAGTGATGAAGATTGTTTGTTATTGTTTAATCAAACTGCATTTTTAGACGACAACGAAACAGACTTTAAATTGTTTGCAGATATTGGAAAGGAGATTGCAAAAAAGTGTCATGGTTTGCCTCTTGCTGCGAAAGCTTTAGGTAGTCTTATGCAGTATAAGAAAACAAGAACTGAATGGCAAAATGTTTTAGATAGTAAGATATGGAATGTAGAGGAGGTTGAACAAGTTTTCCAATCATTGTTACTAAGCTTTTATGATTTGAATCCAGCGGTCAAAACTTGTCTTTTATATTGTGCCACCTTTCAAAAAGAATATGAGTTTGACAAAAATATTTTGATTGAGTTGTGGATGTCACAAGAATATCTTAGTTTAAACCAAGATAAAGAAACAATAATAGGTCAAAATTATTTCAATAGTTTAGTAATGAGATCTTTCTTTCAAGATTTTAAAAAAGATAATGACGGGAATATTACAAAGTGTAAAATGCATAATATTGTGCATGATTTCGTACAGTTTTTCACAGAAAATGAATACTTTATCATGGAGGCTGACAATGCTAATGAGATAAATATCGCACTAGCTAATAAGGTTCGACATTTGACATTGATGTTTTCCCCCGATGGTTCATTTCCTATTTCAATTTACCATTTTAAGAGATTGAGTACTCTCATAGCTTCTTTTTTAAAAATTACTACAATAAACTCAAATTTATTTTTTGAACTGAGATGTCTTAGGACATTAGATTTGAGTCATAACGACATTAAAGAACTTCCACAACAGATTGGCGAACTAAAACTTTTGCGTCATCTTGACTTGTCTTACAATTTTGATTTGGAAGAATTGCCCAATACTGTGGGTGATTTATGTAATCTGCAAACTCTTCGAATCAATAACTGCTGGAAGCTTGAAAAACTACCTGACACTGTGAGAAAATTGATCAACTTAAAGCATCTTCATATTGAGGAATCCAATAAAGTAAAGTTACCAAAAGACATTGGAAAATTGACAAGTCTGCAAACACCACATGGGTTTACTGTCCAGCATTTCAACTTCGATCACTATAAAATATTCACCTTAGAAGATCTGGAGAAGTTGGACCAACTTCAGGGTAGTCTACGCATAAGTGATTTAGAAGGGGATGCAGATATGGCTGAAAAGGCGCAATTGGTGAAGAAGAAACACCTACTTCATTTGTCTTTAGAATTTGGGGTTGCGGATAGTAAACATCAGGAACTATTGCTGAATGCGTTGCAACCACATACCAATTTAGAAGTATTAATGATCCAAGGTTACCGGGGAATGGTACATCCAAATTGGATGATGTCTTTACACAATTTGAGAAGCCTTACTCTTAAAGGCAACGAAAATTGCGAGTTTTTGCCTCCTCTGGGGAAATTGCCATCCCTTGAAGTTTTGAGAATATCATGGTTGGAAAGGGTGAAAAAGCTCGGAGTTGAGTTCTTGGGAATAGAAGAATTACCAAACAGGAAAACATCATCCATTACCATTGGCAATGCCGTTGCTTTCCCCAAATTGAAACAACTCGAGCTGCATTCACTAGATAGCTTGGAAGAGTGGGAAGGAGTCACAGCTGGGTGGAGCGAAGAAGAAGATGATTCAGAACTTACAATAATGCCATCTCTTTCTTCCTTACAAATTGGGTACTGCAATAAGCTGAAAAAACTGCCGGACTTGGTGTGGAAGACACCGCTGCAGAGACTGAGCATTAAATTTTGTTCAATACTCACAGGAACAGGTAGACAGTGGGCCGAGATGTCTCACATCCCAAACATCGAAATCAATAATGTGTTTGTACAGAAAGGAGGAGTTCAGCTTGTGTCGGCAACTGACGAGGACGATGAACACGATGATGAGAGCGAGATCTGCCGTACTGATTCACTTTACTGGATTTCAACAATGCCTGAGGATATATCGACACGGCAGGTGTCAGAGGAGAGATTGACGGGGAAGGTGAAGTGGTTCAACGACTCTAAAGGTTTCGGCTTCATCACCCCGGACGACGACGACAGAGAAGATTTGTTCGTTCACTTCTCGGCGATCAAATGCGACGGCTACCGCACTATCATGGATGGCCTGGCCGTCGAATTCCAAATCGGGGTAGGCCCTCGCGGCTCGACCCAGGCCATCAACGTCACTGGCCTCAATGGCGCTGCTATGCAGGGGCAACGGAAGGAGAGAGTGGAACGGTTCGGTTGGACGAGGCGGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

1091

Amino Acids

125.19

Weight (kDa)

5.72

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 96 8.9e-20 Rx N-terminal domain
AAA_16 PF13191 182 - 324 2.3e-06 AAA ATPase domain
NB-ARC PF00931 186 - 363 6.8e-36 NB-ARC domain
WHD_DRP PF23559 449 - 518 8.2e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 545 - 645 5.4e-11 Leucine-rich repeat region
LRR_8 PF13855 567 - 623 9.1e-07 Leucine rich repeat
LRR_14 PF23598 615 - 901 6.1e-24 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 707 - 823 1.7e-28 R13L1/DRL21 LRRs
CSD PF00313 1003 - 1068 5.8e-22 'Cold-shock' DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000083)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07560 AT1G51790 AT1G51790 AT1G51790 AT1G51800 AT1G51805 AT1G51805 AT1G51810 AT1G51820 AT1G51820 AT1G51830 AT1G51830 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51850 AT1G51850 AT1G51860 AT1G51860 AT1G51870 AT1G51870 AT1G51880 AT1G51880 AT1G51880 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51910 AT2G04300 AT2G04300 AT2G28960 AT2G28960 AT2G28970 AT2G28970 AT2G28990 AT2G28990 AT2G29000 AT3G21340 AT3G46350 AT5G59616 AT5G59650 AT5G59650 AT5G59660 AT5G59660 AT5G59660 AT5G59670 AT5G59670 AT5G59680
fragaria_vesca FvH4_2g03270 FvH4_3g21300 FvH4_6g32660 FvH4_6g32660 FvH4_6g32670 FvH4_6g32670 FvH4_6g32690 FvH4_6g32690 FvH4_6g32691 FvH4_6g32700 FvH4_6g32740 FvH4_6g32750 FvH4_6g33210 FvH4_6g33210 FvH4_7g24270 FvH4_7g24280 FvH4_7g24291 FvH4_7g24300
malus_domestica MD09G1198400.v1.1 MD09G1198600.v1.1 MD09G1199100.v1.1 MD09G1199200.v1.1 MD09G1199300.v1.1 MD09G1199500.v1.1 MD09G1199600.v1.1 MD17G1179300.v1.1 MD17G1180200.v1.1 MD17G1266000.v1.1
prunus_persica Prupe.1G438900_v2.0.a1 Prupe.1G439000_v2.0.a1 Prupe.1G439100_v2.0.a1 Prupe.3G019400_v2.0.a1 Prupe.3G021900_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G055100_v2.0.a1 Prupe.3G055400_v2.0.a1 Prupe.3G055500_v2.0.a1 Prupe.3G055600_v2.0.a1 Prupe.3G055700_v2.0.a1 Prupe.3G055800_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056100_v2.0.a1 Prupe.3G209600_v2.0.a1 Prupe.3G209700_v2.0.a1 Prupe.6G291100_v2.0.a1 Prupe.7G208900_v2.0.a1 Prupe.8G038300_v2.0.a1
pyrus_communis pycom09g11570 pycom09g11580 pycom09g11610 pycom09g11630 pycom09g11640 pycom17g18840 pycom17g18910 pycom17g18930 pycom17g18940
rosa_chinensis RchiOBHm_Chr1g0317521 RchiOBHm_Chr1g0317541 RchiOBHm_Chr2g0123351 RchiOBHm_Chr2g0141181 RchiOBHm_Chr2g0141191 RchiOBHm_Chr2g0141211 RchiOBHm_Chr2g0141221 RchiOBHm_Chr2g0141261 RchiOBHm_Chr2g0141271 RchiOBHm_Chr2g0141311 RchiOBHm_Chr2g0141331 RchiOBHm_Chr2g0141371 RchiOBHm_Chr2g0141391 RchiOBHm_Chr2g0141401 RchiOBHm_Chr2g0141431 RchiOBHm_Chr2g0141481 RchiOBHm_Chr2g0141491 RchiOBHm_Chr2g0141591 RchiOBHm_Chr2g0141601 RchiOBHm_Chr2g0141611 RchiOBHm_Chr2g0141631 RchiOBHm_Chr2g0141641 RchiOBHm_Chr2g0141661 RchiOBHm_Chr2g0141681 RchiOBHm_Chr2g0141701 RchiOBHm_Chr2g0141781 RchiOBHm_Chr2g0141811 RchiOBHm_Chr2g0141821 RchiOBHm_Chr3g0479311 RchiOBHm_Chr3g0495751 RchiOBHm_Chr4g0404741 RchiOBHm_Chr4g0410341 RchiOBHm_Chr4g0418371 RchiOBHm_Chr4g0438651 RchiOBHm_Chr5g0036411 RchiOBHm_Chr5g0036491 RchiOBHm_Chr5g0042391 RchiOBHm_Chr6g0279871 RchiOBHm_Chr7g0192921 RchiOBHm_Chr7g0214901 RchiOBHm_Chr7g0234931
rosa_laevigata RLG00000003432 RLG00000008806 RLG00000013062 RLG00000019514 RLG00000019923 RLG00000019924 RLG00000019925 RLG00000019930 RLG00000019931 RLG00000019934 RLG00000019937 RLG00000019938 RLG00000033697
rosa_multiflora Rmu_co8002302.1_g000001 Rmu_sc0000147.1_g000031 Rmu_sc0000463.1_g000005 Rmu_sc0000712.1_g000003 Rmu_sc0001483.1_g000017 Rmu_sc0001554.1_g000002 Rmu_sc0001554.1_g000005 Rmu_sc0001554.1_g000008 Rmu_sc0001981.1_g000017 Rmu_sc0002040.1_g000056 Rmu_sc0002755.1_g000018 Rmu_sc0002804.1_g000016 Rmu_sc0002928.1_g000015 Rmu_sc0003040.1_g000008 Rmu_sc0003040.1_g000018 Rmu_sc0004512.1_g000001 Rmu_sc0004512.1_g000004 Rmu_sc0005507.1_g000001 Rmu_sc0005507.1_g000009 Rmu_sc0005507.1_g000033 Rmu_sc0005507.1_g000037 Rmu_sc0005507.1_g000043 Rmu_sc0005507.1_g000047 Rmu_sc0006318.1_g000004 Rmu_sc0006318.1_g000014 Rmu_sc0006318.1_g000021 Rmu_sc0006556.1_g000001 Rmu_sc0007208.1_g000002 Rmu_sc0007208.1_g000003 Rmu_sc0007208.1_g000006 Rmu_sc0007208.1_g000008
rosa_roxburghii Rroxscaffold_1G00044430 Rroxscaffold_2G00103420 Rroxscaffold_2G00103470 Rroxscaffold_2G00103490 Rroxscaffold_2G00103500 Rroxscaffold_2G00103520 Rroxscaffold_2G00103530 Rroxscaffold_2G00103540 Rroxscaffold_2G00103550 Rroxscaffold_2G00103570 Rroxscaffold_2G00103770 Rroxscaffold_2G00103780 Rroxscaffold_2G00103800 Rroxscaffold_2G00103820 Rroxscaffold_2G00103860 Rroxscaffold_2G00103890 Rroxscaffold_2G00103930 Rroxscaffold_2G00103940 Rroxscaffold_2G00104010 Rroxscaffold_2G00104030 Rroxscaffold_2G00104050 Rroxscaffold_2G00104060 Rroxscaffold_2G00104070 Rroxscaffold_2G00104090 Rroxscaffold_2G00104100 Rroxscaffold_2G00104120 Rroxscaffold_2G00104130 Rroxscaffold_4G00331710
rosa_rugosa Rorug02G0367500 Rorug02G0367500 Rorug02G0367600 Rorug02G0367800 Rorug02G0367800 Rorug02G0367900 Rorug02G0368100 Rorug02G0368100 Rorug02G0368100 Rorug02G0368200 Rorug02G0368700 Rorug02G0368900 Rorug05G0155600 Rorug05G0155700 Rorug05G0155800 Rorug05G0156700 Rorug05G0157300
rosa_samantha Rh1AG028000 Rh2AG418700 Rh2AG418900 Rh2AG419000 Rh2AG419100 Rh2AG419400 Rh2AG419600 Rh2AG419800 Rh2AG420400 Rh2AG421000 Rh2BG428500 Rh2BG428600 Rh2BG428800 Rh2BG428900 Rh2BG429300 Rh2BG429400 Rh2BG429700 Rh2BG429900 Rh2BG430000 Rh2BG430700 Rh2CG405100 Rh2CG405200 Rh2CG405300 Rh2CG405500 Rh2CG405600 Rh2CG405700 Rh2CG406000 Rh2CG406100 Rh2CG406300 Rh2CG406600 Rh2CG406700 Rh2CG407500 Rh2CG408100 Rh2DG438500 Rh3DG123900 Rh3DG124000 Rh5BG249700 Rh5BG482500 Rh5CG281500 Rh5CG507300 Rh7DG133300
rosa_wichuraiana Rw0G019720 Rw0G019730 Rw0G019750 Rw0G023010 Rw2G034340 Rw2G034350 Rw2G034370 Rw2G034380 Rw2G034400 Rw2G034430 Rw2G034470 Rw2G034480 Rw2G034510 Rw5G022890 Rw5G026410 Rw5G032710 Rw5G042850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2977
Acc36I ACCTGC 1 cut(s) 2977
AccI GTMKAC 2 cut(s) 2158, 2803
AccII CGCG 1 cut(s) 3174
AccIII TCCGGA 1 cut(s) 186
AciI CCGC 7 cut(s) 1313, 2258, 2753, 3121, 3174, 3268, 3271
AclWI GGATC 2 cut(s) 737, 2320
AcoI YGGCCR 1 cut(s) 3141
AcsI RAATTY 6 cut(s) 723, 963, 1750, 2246, 2774, 3149
AcuI CTGAAG 2 cut(s) 27, 2132
AfaI GTAC 7 cut(s) 327, 1554, 1708, 2349, 2708, 2859, 2935
AfiI CCNNNNNNNGG 4 cut(s) 2149, 2344, 2432, 3160
AflIII ACRYGT 2 cut(s) 213, 977
AjiI CACGTC 1 cut(s) 214
AjnI CCWGG 2 cut(s) 3138, 3183
AloI GAACNNNNNNTCC 2 cut(s) 1257, 1289
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 5 cut(s) 70, 157, 997, 2755, 2826
AlwI GGATC 2 cut(s) 737, 2320
AlwNI CAGNNNCTG 1 cut(s) 2764
Ama87I CYCGRG 1 cut(s) 2588
Aor13HI TCCGGA 1 cut(s) 186
AoxI GGCC 6 cut(s) 2811, 3136, 3141, 3166, 3186, 3202
ApeKI GCWGC 4 cut(s) 1169, 2593, 2755, 3215
ApoI RAATTY 6 cut(s) 723, 963, 1750, 2246, 2774, 3149
ArsI GACNNNNNNTTYG 4 cut(s) 203, 235, 1771, 1803
AseI ATTAAT 1 cut(s) 2321
Asp700I GAANNNNTTC 2 cut(s) 132, 3023
AspLEI GCGC 2 cut(s) 2206, 3215
AspS9I GGNCC 3 cut(s) 2140, 2811, 3167
AsuC2I CCSGG 2 cut(s) 2339, 3059
AsuHPI GGTGA 8 cut(s) 85, 308, 1913, 2107, 2224, 2494, 3028, 3046
AsuII TTCGAA 1 cut(s) 1927
AvaI CYCGRG 1 cut(s) 2588
AvaII GGWCC 1 cut(s) 2140
AxyI CCTNAGG 1 cut(s) 2967
BbsI GAAGAC 2 cut(s) 513, 2753
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 4 cut(s) 1156, 2580, 2742, 3202
BccI CCATC 9 cut(s) 55, 129, 640, 782, 1658, 2452, 2689, 3128, 3197
BceAI ACGGC 5 cut(s) 2546, 2916, 2999, 3128, 3130
BcgI CGANNNNNNTGC 2 cut(s) 1907, 1941
BciT130I CCWGG 2 cut(s) 3140, 3185
BclI TGATCA 1 cut(s) 1980
BcnI CCSGG 2 cut(s) 2339, 3059
BcoDI GTCTC 5 cut(s) 70, 157, 997, 2755, 2826
BfaI CTAG 4 cut(s) 348, 540, 1622, 2603
BfmI CTRYAG 2 cut(s) 306, 2756
BfoI RGCGCY 1 cut(s) 3216
BfuAI ACCTGC 1 cut(s) 2977
BglI GCCNNNNNGGC 2 cut(s) 3174, 3210
BglII AGATCT 3 cut(s) 1472, 2125, 2925
BisI GCNGC 6 cut(s) 1170, 2594, 2756, 3175, 3216, 3269
BlsI GCNGC 6 cut(s) 1171, 2595, 2757, 3176, 3217, 3270
BmcAI AGTACT 1 cut(s) 1708
Bme1390I CCNGG 4 cut(s) 2339, 3059, 3140, 3185
Bme18I GGWCC 1 cut(s) 2140
BmeT110I CYCGRG 1 cut(s) 2588
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 3 cut(s) 2140, 2811, 3167
BmiI GGNNCC 2 cut(s) 177, 899
BmrFI CCNGG 4 cut(s) 2339, 3059, 3140, 3185
BmsI GCATC 2 cut(s) 2002, 2173
BpiI GAAGAC 2 cut(s) 513, 2753
BpmI CTGGAG 1 cut(s) 2150
Bpu14I TTCGAA 1 cut(s) 1927
BpuMI CCSGG 2 cut(s) 2339, 3059
BsaAI YACGTR 1 cut(s) 980
BsaBI GATNNNNATC 2 cut(s) 1305, 1613
BsaJI CCNNGG 4 cut(s) 2329, 2338, 3057, 3183
BsaWI WCCGGW 2 cut(s) 186, 953
Bsc4I CCNNNNNNNGG 4 cut(s) 2149, 2344, 2432, 3160
Bse1I ACTGG 2 cut(s) 2954, 3205
Bse21I CCTNAGG 1 cut(s) 2967
Bse3DI GCAATG 3 cut(s) 402, 499, 2563
Bse8I GATNNNNATC 2 cut(s) 1305, 1613
BseAI TCCGGA 1 cut(s) 186
BseBI CCWGG 2 cut(s) 3140, 3185
BseDI CCNNGG 4 cut(s) 2329, 2338, 3057, 3183
BseJI GATNNNNATC 2 cut(s) 1305, 1613
BseLI CCNNNNNNNGG 4 cut(s) 2149, 2344, 2432, 3160
BseMI GCAATG 3 cut(s) 402, 499, 2563
BseMII CTCAG 3 cut(s) 1757, 2756, 2958
BseNI ACTGG 2 cut(s) 2954, 3205
BseRI GAGGAG 6 cut(s) 173, 631, 1271, 2418, 2883, 3011
BseXI GCAGC 4 cut(s) 1156, 2580, 2742, 3202
BseYI CCCAGC 1 cut(s) 2636
Bsh1236I CGCG 1 cut(s) 3174
BshFI GGCC 6 cut(s) 2813, 3138, 3143, 3168, 3188, 3204
BsiHKAI GWGCWC 1 cut(s) 147
BsiHKCI CYCGRG 1 cut(s) 2588
BsiSI CCGG 6 cut(s) 179, 187, 954, 2338, 2732, 3059
BslFI GGGAC 1 cut(s) 339
BslI CCNNNNNNNGG 4 cut(s) 2149, 2344, 2432, 3160
BsmAI GTCTC 5 cut(s) 70, 157, 997, 2755, 2826
BsmFI GGGAC 1 cut(s) 339
BsmI GAATGC 3 cut(s) 157, 2293, 2596
BsnI GGCC 6 cut(s) 2813, 3138, 3143, 3168, 3188, 3204
BsoBI CYCGRG 1 cut(s) 2588
Bsp119I TTCGAA 1 cut(s) 1927
Bsp1286I GDGCHC 1 cut(s) 147
Bsp13I TCCGGA 1 cut(s) 186
Bsp1407I TGTACA 1 cut(s) 2857
BspACI CCGC 7 cut(s) 1313, 2258, 2753, 3121, 3174, 3268, 3271
BspANI GGCC 6 cut(s) 2813, 3138, 3143, 3168, 3188, 3204
BspCNI CTCAG 3 cut(s) 1758, 2757, 2959
BspEI TCCGGA 1 cut(s) 186
BspFNI CGCG 1 cut(s) 3174
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 2 cut(s) 177, 899
BspMAI CTGCAG 1 cut(s) 2760
BspMI ACCTGC 1 cut(s) 2977
BspPI GGATC 2 cut(s) 737, 2320
BspT104I TTCGAA 1 cut(s) 1927
BsrDI GCAATG 3 cut(s) 402, 499, 2563
BsrGI TGTACA 1 cut(s) 2857
BsrI ACTGG 2 cut(s) 2954, 3205
BssECI CCNNGG 4 cut(s) 2329, 2338, 3057, 3183
BssT1I CCWWGG 1 cut(s) 2329
Bst2UI CCWGG 2 cut(s) 3140, 3185
Bst4CI ACNGT 6 cut(s) 1557, 1897, 1969, 2080, 2808, 3252
Bst6I CTCTTC 2 cut(s) 1929, 2610
BstAUI TGTACA 1 cut(s) 2857
BstBAI YACGTR 1 cut(s) 980
BstBI TTCGAA 1 cut(s) 1927
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 9 cut(s) 408, 912, 1289, 1409, 1766, 1775, 2119, 2765, 2967
BstEII GGTNACC 1 cut(s) 2333
BstFNI CGCG 1 cut(s) 3174
BstH2I RGCGCY 1 cut(s) 3216
BstHHI GCGC 2 cut(s) 2206, 3215
BstMAI GTCTC 5 cut(s) 70, 157, 997, 2755, 2826
BstNI CCWGG 2 cut(s) 3140, 3185
BstPI GGTNACC 1 cut(s) 2333
BstSCI CCNGG 4 cut(s) 2337, 3057, 3138, 3183
BstSFI CTRYAG 2 cut(s) 306, 2756
BstUI CGCG 1 cut(s) 3174
BstV1I GCAGC 4 cut(s) 1156, 2580, 2742, 3202
BstV2I GAAGAC 2 cut(s) 513, 2753
BstX2I RGATCY 3 cut(s) 1472, 2125, 2925
BstXI CCANNNNNNTGG 1 cut(s) 1897
BstYI RGATCY 3 cut(s) 1472, 2125, 2925
Bsu36I CCTNAGG 1 cut(s) 2967
BsuRI GGCC 6 cut(s) 2813, 3138, 3143, 3168, 3188, 3204
BtrI CACGTC 1 cut(s) 214
BtsI GCAGTG 1 cut(s) 845
BtsIMutI CAGTG 4 cut(s) 845, 1965, 2813, 3198
BveI ACCTGC 1 cut(s) 2977
Cac8I GCNNGC 1 cut(s) 39
CaiI CAGNNNCTG 1 cut(s) 2764
CciI TCATGA 1 cut(s) 397
CfoI GCGC 2 cut(s) 2206, 3215
Cfr13I GGNCC 3 cut(s) 2140, 2811, 3167
CseI GACGC 2 cut(s) 383, 1835
Csp6I GTAC 7 cut(s) 326, 1553, 1707, 2348, 2707, 2858, 2934
CviQI GTAC 7 cut(s) 326, 1553, 1707, 2348, 2707, 2858, 2934
DdeI CTNAG 9 cut(s) 408, 912, 1289, 1409, 1766, 1775, 2119, 2765, 2967
DraI TTTAAA 4 cut(s) 1111, 1416, 1492, 1728
EaeI YGGCCR 1 cut(s) 3141
Eam1104I CTCTTC 2 cut(s) 1929, 2610
EarI CTCTTC 2 cut(s) 1929, 2610
Eco130I CCWWGG 1 cut(s) 2329
Eco47I GGWCC 1 cut(s) 2140
Eco57I CTGAAG 2 cut(s) 27, 2132
Eco81I CCTNAGG 1 cut(s) 2967
Eco88I CYCGRG 1 cut(s) 2588
Eco91I GGTNACC 1 cut(s) 2333
EcoO109I RGGNCCY 1 cut(s) 3167
EcoO65I GGTNACC 1 cut(s) 2333
EcoRI GAATTC 1 cut(s) 3149
EcoRII CCWGG 2 cut(s) 3138, 3183
EcoT14I CCWWGG 1 cut(s) 2329
EcoT22I ATGCAT 1 cut(s) 1533
ErhI CCWWGG 1 cut(s) 2329
FalI AAGNNNNNCTT 4 cut(s) 424, 456, 1392, 1424
FaqI GGGAC 1 cut(s) 339
FbaI TGATCA 1 cut(s) 1980
FblI GTMKAC 2 cut(s) 2158, 2803
Fnu4HI GCNGC 6 cut(s) 1170, 2594, 2756, 3175, 3216, 3269
Fsp4HI GCNGC 6 cut(s) 1170, 2594, 2756, 3175, 3216, 3269
FspBI CTAG 4 cut(s) 348, 540, 1622, 2603
GlaI GCGC 2 cut(s) 2205, 3214
GluI GCNGC 6 cut(s) 1170, 2594, 2756, 3175, 3216, 3269
GsaI CCCAGC 1 cut(s) 2640
GsuI CTGGAG 1 cut(s) 2150
HaeII RGCGCY 1 cut(s) 3216
HaeIII GGCC 6 cut(s) 2813, 3138, 3143, 3168, 3188, 3204
HapII CCGG 6 cut(s) 179, 187, 954, 2338, 2732, 3059
HgaI GACGC 2 cut(s) 383, 1835
HhaI GCGC 2 cut(s) 2206, 3215
Hin6I GCGC 2 cut(s) 2204, 3213
HinP1I GCGC 2 cut(s) 2204, 3213
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 4 cut(s) 933, 1176, 1291, 1946
HpaII CCGG 6 cut(s) 179, 187, 954, 2338, 2732, 3059
HphI GGTGA 8 cut(s) 85, 308, 1913, 2107, 2224, 2494, 3028, 3046
Hpy166II GTNNAC 6 cut(s) 94, 2076, 2159, 2267, 2804, 3091
Hpy188I TCNGA 7 cut(s) 114, 742, 850, 1035, 2495, 2665, 2995
Hpy8I GTNNAC 6 cut(s) 94, 2076, 2159, 2267, 2804, 3091
Hpy99I CGWCG 5 cut(s) 236, 3068, 3071, 3116, 3149
HpyAV CCTTC 6 cut(s) 316, 605, 2171, 2618, 3007, 3229
HpyCH4III ACNGT 6 cut(s) 1557, 1897, 1969, 2080, 2808, 3252
HpyCH4IV ACGT 6 cut(s) 213, 234, 328, 518, 979, 3195
HpyF3I CTNAG 9 cut(s) 408, 912, 1289, 1409, 1766, 1775, 2119, 2765, 2967
HpySE526I ACGT 6 cut(s) 213, 234, 328, 518, 979, 3195
HspAI GCGC 2 cut(s) 2204, 3213
Kpn2I TCCGGA 1 cut(s) 186
Ksp22I TGATCA 1 cut(s) 1980
LmnI GCTCC 3 cut(s) 150, 175, 2643
Lsp1109I GCAGC 4 cut(s) 1156, 2580, 2742, 3202
LweI GCATC 2 cut(s) 2002, 2173
MaeI CTAG 4 cut(s) 348, 540, 1622, 2603
MaeII ACGT 6 cut(s) 213, 234, 328, 518, 979, 3195
MaeIII GTNAC 9 cut(s) 822, 970, 1009, 1284, 1395, 2025, 2333, 2629, 3196
MfeI CAATTG 3 cut(s) 361, 677, 2207
MflI RGATCY 3 cut(s) 1472, 2125, 2925
MhlI GDGCHC 1 cut(s) 147
MlyI GAGTC 4 cut(s) 628, 1801, 2637, 3026
MmeI TCCRAC 4 cut(s) 45, 2118, 2454, 3239
Mph1103I ATGCAT 1 cut(s) 1533
MroI TCCGGA 1 cut(s) 186
MroXI GAANNNNTTC 2 cut(s) 132, 3023
MslI CAYNNNNRTG 2 cut(s) 2913, 3128
MspA1I CMGCKG 3 cut(s) 1313, 2636, 2755
MspI CCGG 6 cut(s) 179, 187, 954, 2338, 2732, 3059
MspR9I CCNGG 4 cut(s) 2339, 3059, 3140, 3185
MssI GTTTAAAC 1 cut(s) 1416
MunI CAATTG 3 cut(s) 361, 677, 2207
Mva1269I GAATGC 3 cut(s) 157, 2293, 2596
MvaI CCWGG 2 cut(s) 3140, 3185
MvnI CGCG 1 cut(s) 3174
NciI CCSGG 2 cut(s) 2339, 3059
NlaIV GGNNCC 2 cut(s) 177, 899
NmeAIII GCCGAG 3 cut(s) 830, 2839, 3077
NmuCI GTSAC 4 cut(s) 970, 1395, 2629, 3196
NsiI ATGCAT 1 cut(s) 1533
NspV TTCGAA 1 cut(s) 1927
PaeR7I CTCGAG 1 cut(s) 2588
PagI TCATGA 1 cut(s) 397
PaqCI CACCTGC 1 cut(s) 2977
PcsI WCGNNNNNNNCGW 3 cut(s) 240, 2412, 2907
PctI GAATGC 3 cut(s) 157, 2293, 2596
PdmI GAANNNNTTC 2 cut(s) 132, 3023
PfeI GAWTC 6 cut(s) 259, 1306, 1929, 2009, 2660, 2939
PkrI GCNGC 6 cut(s) 1171, 2595, 2757, 3176, 3217, 3270
PleI GAGTC 4 cut(s) 627, 1800, 2636, 3026
PmeI GTTTAAAC 1 cut(s) 1416
PpsI GAGTC 4 cut(s) 627, 1800, 2636, 3026
Ppu21I YACGTR 1 cut(s) 980
PshBI ATTAAT 1 cut(s) 2321
Psp6I CCWGG 2 cut(s) 3138, 3183
PspEI GGTNACC 1 cut(s) 2333
PspFI CCCAGC 1 cut(s) 2636
PspGI CCWGG 2 cut(s) 3138, 3183
PspN4I GGNNCC 2 cut(s) 177, 899
PspPI GGNCC 3 cut(s) 2140, 2811, 3167
PspXI VCTCGAGB 1 cut(s) 2588
PstI CTGCAG 1 cut(s) 2760
PstNI CAGNNNCTG 1 cut(s) 2764
PsuI RGATCY 3 cut(s) 1472, 2125, 2925
PvuII CAGCTG 1 cut(s) 2636
RsaI GTAC 7 cut(s) 327, 1554, 1708, 2349, 2708, 2859, 2935
RsaNI GTAC 7 cut(s) 326, 1553, 1707, 2348, 2707, 2858, 2934
RseI CAYNNNNRTG 2 cut(s) 2913, 3128
SatI GCNGC 6 cut(s) 1170, 2594, 2756, 3175, 3216, 3269
Sau96I GGNCC 3 cut(s) 2140, 2811, 3167
ScaI AGTACT 1 cut(s) 1708
SchI GAGTC 4 cut(s) 628, 1801, 2637, 3026
ScrFI CCNGG 4 cut(s) 2339, 3059, 3140, 3185
SduI GDGCHC 1 cut(s) 147
SfaNI GCATC 2 cut(s) 2002, 2173
SfcI CTRYAG 2 cut(s) 306, 2756
Sfr274I CTCGAG 1 cut(s) 2588
SfuI TTCGAA 1 cut(s) 1927
SinI GGWCC 1 cut(s) 2140
SlaI CTCGAG 1 cut(s) 2588
SmiMI CAYNNNNRTG 2 cut(s) 2913, 3128
SmlI CTYRAG 1 cut(s) 2588
SmoI CTYRAG 1 cut(s) 2588
SsiI CCGC 7 cut(s) 1313, 2258, 2753, 3121, 3174, 3268, 3271
SspI AATATT 4 cut(s) 1375, 1513, 1537, 2112
SspMI CTAG 4 cut(s) 348, 540, 1622, 2603
StyD4I CCNGG 4 cut(s) 2337, 3057, 3138, 3183
StyI CCWWGG 1 cut(s) 2329
TaaI ACNGT 6 cut(s) 1557, 1897, 1969, 2080, 2808, 3252
TaiI ACGT 6 cut(s) 216, 237, 331, 521, 982, 3198
TaqI TCGA 9 cut(s) 126, 1636, 1927, 2097, 2589, 2838, 2978, 3147, 3179
TatI WGTACW 2 cut(s) 1706, 2857
TauI GCSGC 2 cut(s) 3177, 3271
TfiI GAWTC 6 cut(s) 259, 1306, 1929, 2009, 2660, 2939
TscAI CASTG 4 cut(s) 845, 1972, 2813, 3205
TseFI GTSAC 4 cut(s) 970, 1395, 2629, 3196
TseI GCWGC 4 cut(s) 1169, 2593, 2755, 3215
Tsp45I GTSAC 4 cut(s) 970, 1395, 2629, 3196
TspGWI ACGGA 1 cut(s) 3247
TspRI CASTG 4 cut(s) 845, 1972, 2813, 3205
VpaK11BI GGWCC 1 cut(s) 2140
VspI ATTAAT 1 cut(s) 2321
XapI RAATTY 6 cut(s) 723, 963, 1750, 2246, 2774, 3149
XbaI TCTAGA 1 cut(s) 539
XhoI CTCGAG 1 cut(s) 2588
XmiI GTMKAC 2 cut(s) 2158, 2803
XmnI GAANNNNTTC 2 cut(s) 132, 3023
XspI CTAG 4 cut(s) 348, 540, 1622, 2603
ZrmI AGTACT 1 cut(s) 1708
Zsp2I ATGCAT 1 cut(s) 1533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.