MD09G1199300.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000083
Physical Location & Seq
Forward (+)
18239158 .. 18256339
17182 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1199300.v1.1.491

Sequence Viewer

Length: 2673 bp
ATGCTACAGAACCAATATTTGGTGTCTTGTGTCCCAAAGATGTCACGGACAATGAAGCAACTCTTCCTCTTCGCAGCGCCGCTTGGTGGTTTCGCTCTTAATCTTTTGTTCCTAGTTCATGCCCAAGATCAATCAGGCTTCATTAGCATAGATTGTGGCCTAAGGGAAGACTTCAGGTATAATTCAACATGGTCAGGCATTACCTATATTTCAGACGAAAACTTCGTGGCCACTGGTGAACGGAAGTTGGTGTTGCCTGAACACAAAAACACGTATTCCCCGTTATACAACTCTCTTAGAAGCTTCCCTAAAGGTATCCGAAACTGCTACAAAATAAATGTTACAAGTAAAACCAAGTATTTGATCAGAGCAGGTTTCTTTTATGGGAATTATGATGGACAAAATGAAGTACCCCAATTTGAGATACATCTTGGACCTAACTTGTGGGATACAGTGAAGTCGGAAGCAACAAAAATCGAGCTCATACATGTCCCTCTGCAAAACTATGTACATGTTTGTCTGGTGAAGAAAGGCTCCGGGGTTCCATTTATATCAGTCATAGAACTCAGGCCATTAACCGATGCCTCGTACAAAACAGTGAAAGGGTCCTTATCACTTCTCTTGCGGAGTGACACTGGTCTAACAGCTGACCGTGAATACAGGTATCCATTTGATACTCATGATCGAATCTGGGAATACTTTGACGACCACAGCTCTTGGACAAAATTAAATACCTCATCCACCATCAATTCAGAATTCGAGTATCAACTACCATCCGTCGTGATGGGCACAGCTGCAACGCCGACAAGTTCAAATGATTCGTTGAATATTTTATGGGACCCGTATGATGACACTGAAGTACATCATGTTTACTTGCACTTTGCAGAAGTTGAAAAGCTCCAACCCAATCAGTCTAGACAGTTCAACATTACTACAAACGGAGAGCTGTGTTATGGGACACTTGCTCCTGATTATTTGTCCACAACGACAATATTGTGCACCGCTAAATCCCTGAGTGGACCAGGAGTAAACAGTAATTTTTCAATTGTTAAGACTGGAAACTCTACCCTTCCACCCATCCTCAATGCCTACGAGATTTATGGAGTGAAGGAATTCTTAACATCAGATACCAACCAAGACGATGTTGAGGCTATCACAAACATTAAGTCAACGTATAATATTGAAAATAACTGGCAAGGAGATCCTTGTAACCCTCATGTTTACTCATGGGATGGTCTAAACTGTAGCTATCAAGGAAATGATCCCCCAAGAATTATATCCTTGAACTTGTCTTCCAGTGGATTAGAAGGGGAGATTTCTCCTTCTCTGTCAAATCTAACAATGATACAGACATTGGATTTATCAAACAACAACTTAAGAGGATCAATTCCAGAAATTTTGTCACAACTGCAGATGTTAAATGTGTTAAATTTGGAGAATAACAACCTCACAGGCTCAATGCCGGTGGTACTCATTGACAGATGGAAGGATGGTTTACTATCATTAAGTTCGTGCGAAAATCCAAACCTATCCGGAAATGTTTCTTGCAACAAGAAGAAGAACAGGAAGTATAATTTTTTTGTTCCGATAATAAGTGTTTTGGTTGTTGCACTGTCAGTCCTCTTACTCAGTGCAGCAGCTATCTGGTGTCGGATTACTTATAGGAGGAAAAGGCAACTTGGAAATAGCACAGATGCAAATGTAGTCATTCAGCATGGGTCATTTGAGCGAAAAGGGAGACAGTTTACGTACTCTGAGATAGTACAGATTACAAGGGGGTTCAAGATGGTTCTTGGAAAAGGCGGCTTTGGAACAGTTTATCACGGCCACATAGACGACACTCAGGTAGCTGTCAAGATGCTTTCACGGTCATCAGTGCAAGGGTTTCAGCAATTTCTCACAGAGGTTGATCTTCTTATGAGAGTTCATCATAGAAATTTGACAAGTCTTGTTGGATATTGCAATGATGAAACCAATATAGGGCTGATCTACGAGTACATGGCCAATGGAAACCTACGAGAACATCTTTCAGGTAGCAGATCAAATATCTTGAGTTGGGGAGATAGACTTCAAATAGCAATAGAAGCTGCACAAGGACTGGAGTATTTGCACTATGGATGCAGTCCGCCTATAATCCACAGGGATGTGAAATCAACAAACATCTTGCTGAATGAAAAATTCGAAGCCAAACTATCTGATTTTGGCATATCCAGAAGTTTCCCTACAGAGGATGGAACGCATATAACAACAAATATCGCTGGCACTCCCGGGTACCTTGACCCTGAGTATTACGCATCATACAGATTGAATAAAGAAAGTGATGTTTATAGCTTTGGCATTGTACTGTTGGAGATTATCACAAGTCGACCTGTTTTTTCTAGAACGCATGAGCGGAGTTACATTAGTGAATGGTTCAGTTTCATGCTTCAAAAAGGAGACATTTACAGTCTTGTTGATCCGAGGTTAGAAGGAAATTTCTGTACGAACTCAGTCTGGAAAGCTGTGGAAATAGCAAATGCATGCGTATCTCCAACTGCCATCAAAAGGCTAAGCATGAGTCAGGTAGTGGTGGATCTAAAGGAGTGCTTGGCAACAGAACTCGCTCAAACAAACCAGAGGGATGAAACTGAACTAAGAAATTCCATTGCTATGCTACACCCCTCAGTTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

891

Amino Acids

100.14

Weight (kDa)

6.09

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 50 - 368 2.4e-104 Malectin-like domain
PK_Tyr_Ser-Thr PF07714 595 - 790 9.3e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 596 - 856 2.2e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000083)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07560 AT1G51790 AT1G51790 AT1G51790 AT1G51800 AT1G51805 AT1G51805 AT1G51810 AT1G51820 AT1G51820 AT1G51830 AT1G51830 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51850 AT1G51850 AT1G51860 AT1G51860 AT1G51870 AT1G51870 AT1G51880 AT1G51880 AT1G51880 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51910 AT2G04300 AT2G04300 AT2G28960 AT2G28960 AT2G28970 AT2G28970 AT2G28990 AT2G28990 AT2G29000 AT3G21340 AT3G46350 AT5G59616 AT5G59650 AT5G59650 AT5G59660 AT5G59660 AT5G59660 AT5G59670 AT5G59670 AT5G59680
fragaria_vesca FvH4_2g03270 FvH4_3g21300 FvH4_6g32660 FvH4_6g32660 FvH4_6g32670 FvH4_6g32670 FvH4_6g32690 FvH4_6g32690 FvH4_6g32691 FvH4_6g32700 FvH4_6g32740 FvH4_6g32750 FvH4_6g33210 FvH4_6g33210 FvH4_7g24270 FvH4_7g24280 FvH4_7g24291 FvH4_7g24300
malus_domestica MD09G1198400.v1.1 MD09G1198600.v1.1 MD09G1199100.v1.1 MD09G1199200.v1.1 MD09G1199300.v1.1 MD09G1199500.v1.1 MD09G1199600.v1.1 MD17G1179300.v1.1 MD17G1180200.v1.1 MD17G1266000.v1.1
prunus_persica Prupe.1G438900_v2.0.a1 Prupe.1G439000_v2.0.a1 Prupe.1G439100_v2.0.a1 Prupe.3G019400_v2.0.a1 Prupe.3G021900_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G055100_v2.0.a1 Prupe.3G055400_v2.0.a1 Prupe.3G055500_v2.0.a1 Prupe.3G055600_v2.0.a1 Prupe.3G055700_v2.0.a1 Prupe.3G055800_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056100_v2.0.a1 Prupe.3G209600_v2.0.a1 Prupe.3G209700_v2.0.a1 Prupe.6G291100_v2.0.a1 Prupe.7G208900_v2.0.a1 Prupe.8G038300_v2.0.a1
pyrus_communis pycom09g11570 pycom09g11580 pycom09g11610 pycom09g11630 pycom09g11640 pycom17g18840 pycom17g18910 pycom17g18930 pycom17g18940
rosa_chinensis RchiOBHm_Chr1g0317521 RchiOBHm_Chr1g0317541 RchiOBHm_Chr2g0123351 RchiOBHm_Chr2g0141181 RchiOBHm_Chr2g0141191 RchiOBHm_Chr2g0141211 RchiOBHm_Chr2g0141221 RchiOBHm_Chr2g0141261 RchiOBHm_Chr2g0141271 RchiOBHm_Chr2g0141311 RchiOBHm_Chr2g0141331 RchiOBHm_Chr2g0141371 RchiOBHm_Chr2g0141391 RchiOBHm_Chr2g0141401 RchiOBHm_Chr2g0141431 RchiOBHm_Chr2g0141481 RchiOBHm_Chr2g0141491 RchiOBHm_Chr2g0141591 RchiOBHm_Chr2g0141601 RchiOBHm_Chr2g0141611 RchiOBHm_Chr2g0141631 RchiOBHm_Chr2g0141641 RchiOBHm_Chr2g0141661 RchiOBHm_Chr2g0141681 RchiOBHm_Chr2g0141701 RchiOBHm_Chr2g0141781 RchiOBHm_Chr2g0141811 RchiOBHm_Chr2g0141821 RchiOBHm_Chr3g0479311 RchiOBHm_Chr3g0495751 RchiOBHm_Chr4g0404741 RchiOBHm_Chr4g0410341 RchiOBHm_Chr4g0418371 RchiOBHm_Chr4g0438651 RchiOBHm_Chr5g0036411 RchiOBHm_Chr5g0036491 RchiOBHm_Chr5g0042391 RchiOBHm_Chr6g0279871 RchiOBHm_Chr7g0192921 RchiOBHm_Chr7g0214901 RchiOBHm_Chr7g0234931
rosa_laevigata RLG00000003432 RLG00000008806 RLG00000013062 RLG00000019514 RLG00000019923 RLG00000019924 RLG00000019925 RLG00000019930 RLG00000019931 RLG00000019934 RLG00000019937 RLG00000019938 RLG00000033697
rosa_multiflora Rmu_co8002302.1_g000001 Rmu_sc0000147.1_g000031 Rmu_sc0000463.1_g000005 Rmu_sc0000712.1_g000003 Rmu_sc0001483.1_g000017 Rmu_sc0001554.1_g000002 Rmu_sc0001554.1_g000005 Rmu_sc0001554.1_g000008 Rmu_sc0001981.1_g000017 Rmu_sc0002040.1_g000056 Rmu_sc0002755.1_g000018 Rmu_sc0002804.1_g000016 Rmu_sc0002928.1_g000015 Rmu_sc0003040.1_g000008 Rmu_sc0003040.1_g000018 Rmu_sc0004512.1_g000001 Rmu_sc0004512.1_g000004 Rmu_sc0005507.1_g000001 Rmu_sc0005507.1_g000009 Rmu_sc0005507.1_g000033 Rmu_sc0005507.1_g000037 Rmu_sc0005507.1_g000043 Rmu_sc0005507.1_g000047 Rmu_sc0006318.1_g000004 Rmu_sc0006318.1_g000014 Rmu_sc0006318.1_g000021 Rmu_sc0006556.1_g000001 Rmu_sc0007208.1_g000002 Rmu_sc0007208.1_g000003 Rmu_sc0007208.1_g000006 Rmu_sc0007208.1_g000008
rosa_roxburghii Rroxscaffold_1G00044430 Rroxscaffold_2G00103420 Rroxscaffold_2G00103470 Rroxscaffold_2G00103490 Rroxscaffold_2G00103500 Rroxscaffold_2G00103520 Rroxscaffold_2G00103530 Rroxscaffold_2G00103540 Rroxscaffold_2G00103550 Rroxscaffold_2G00103570 Rroxscaffold_2G00103770 Rroxscaffold_2G00103780 Rroxscaffold_2G00103800 Rroxscaffold_2G00103820 Rroxscaffold_2G00103860 Rroxscaffold_2G00103890 Rroxscaffold_2G00103930 Rroxscaffold_2G00103940 Rroxscaffold_2G00104010 Rroxscaffold_2G00104030 Rroxscaffold_2G00104050 Rroxscaffold_2G00104060 Rroxscaffold_2G00104070 Rroxscaffold_2G00104090 Rroxscaffold_2G00104100 Rroxscaffold_2G00104120 Rroxscaffold_2G00104130 Rroxscaffold_4G00331710
rosa_rugosa Rorug02G0367500 Rorug02G0367500 Rorug02G0367600 Rorug02G0367800 Rorug02G0367800 Rorug02G0367900 Rorug02G0368100 Rorug02G0368100 Rorug02G0368100 Rorug02G0368200 Rorug02G0368700 Rorug02G0368900 Rorug05G0155600 Rorug05G0155700 Rorug05G0155800 Rorug05G0156700 Rorug05G0157300
rosa_samantha Rh1AG028000 Rh2AG418700 Rh2AG418900 Rh2AG419000 Rh2AG419100 Rh2AG419400 Rh2AG419600 Rh2AG419800 Rh2AG420400 Rh2AG421000 Rh2BG428500 Rh2BG428600 Rh2BG428800 Rh2BG428900 Rh2BG429300 Rh2BG429400 Rh2BG429700 Rh2BG429900 Rh2BG430000 Rh2BG430700 Rh2CG405100 Rh2CG405200 Rh2CG405300 Rh2CG405500 Rh2CG405600 Rh2CG405700 Rh2CG406000 Rh2CG406100 Rh2CG406300 Rh2CG406600 Rh2CG406700 Rh2CG407500 Rh2CG408100 Rh2DG438500 Rh3DG123900 Rh3DG124000 Rh5BG249700 Rh5BG482500 Rh5CG281500 Rh5CG507300 Rh7DG133300
rosa_wichuraiana Rw0G019720 Rw0G019730 Rw0G019750 Rw0G023010 Rw2G034340 Rw2G034350 Rw2G034370 Rw2G034380 Rw2G034400 Rw2G034430 Rw2G034470 Rw2G034480 Rw2G034510 Rw5G022890 Rw5G026410 Rw5G032710 Rw5G042850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 362
Acc65I GGTACC 1 cut(s) 2271
AccB1I GGYRCC 1 cut(s) 2271
AccB7I CCANNNNNTGG 1 cut(s) 19
AccBSI CCGCTC 1 cut(s) 2392
AccI GTMKAC 1 cut(s) 2365
AccIII TCCGGA 1 cut(s) 1532
AciI CCGC 6 cut(s) 80, 625, 1002, 1803, 2126, 2392
AclWI GGATC 5 cut(s) 1196, 1256, 1390, 2450, 2580
AcoI YGGCCR 3 cut(s) 228, 1825, 2001
AcsI RAATTY 8 cut(s) 755, 1112, 1395, 1429, 1936, 2177, 2473, 2638
AcuI CTGAAG 2 cut(s) 157, 876
AfiI CCNNNNNNNGG 6 cut(s) 19, 86, 1980, 2227, 2544, 2667
AflII CTTAAG 1 cut(s) 1375
AflIII ACRYGT 3 cut(s) 270, 487, 511
AjnI CCWGG 1 cut(s) 1021
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AloI GAACNNNNNNTCC 2 cut(s) 1277, 1309
Alw21I GWGCWC 2 cut(s) 483, 1001
Alw26I GTCTC 2 cut(s) 1732, 2430
Alw44I GTGCAC 1 cut(s) 997
AlwI GGATC 5 cut(s) 1196, 1256, 1390, 2450, 2580
Ama87I CYCGRG 1 cut(s) 2267
Aor13HI TCCGGA 1 cut(s) 1532
AoxI GGCC 5 cut(s) 157, 228, 569, 1825, 2001
ApaLI GTGCAC 1 cut(s) 997
ApeKI GCWGC 5 cut(s) 74, 794, 1634, 1637, 2087
ApoI RAATTY 8 cut(s) 755, 1112, 1395, 1429, 1936, 2177, 2473, 2638
ArsI GACNNNNNNTTYG 4 cut(s) 206, 238, 1151, 1183
Asp700I GAANNNNTTC 2 cut(s) 1112, 1540
Asp718I GGTACC 1 cut(s) 2271
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 4 cut(s) 434, 606, 838, 1019
AsuC2I CCSGG 3 cut(s) 538, 2268, 2269
AsuHPI GGTGA 2 cut(s) 248, 535
AsuII TTCGAA 1 cut(s) 2181
AvaI CYCGRG 1 cut(s) 2267
AvaII GGWCC 4 cut(s) 434, 606, 838, 1019
AxyI CCTNAGG 1 cut(s) 161
BaeGI GKGCMC 2 cut(s) 791, 1001
BalI TGGCCA 2 cut(s) 230, 2003
BanI GGYRCC 1 cut(s) 2271
BanII GRGCYC 1 cut(s) 483
BarI GAAGNNNNNNTAC 2 cut(s) 2206, 2238
BbsI GAAGAC 2 cut(s) 174, 1284
Bbv12I GWGCWC 2 cut(s) 483, 1001
BbvI GCAGC 5 cut(s) 86, 781, 1646, 1649, 2074
BceAI ACGGC 1 cut(s) 1840
BciT130I CCWGG 1 cut(s) 1023
BciVI GTATCC 3 cut(s) 326, 442, 675
BclI TGATCA 1 cut(s) 363
BcnI CCSGG 3 cut(s) 538, 2268, 2269
BcoDI GTCTC 2 cut(s) 1732, 2430
BfaI CTAG 3 cut(s) 113, 915, 2379
BfmI CTRYAG 4 cut(s) 5, 1243, 1409, 2223
BfoI RGCGCY 1 cut(s) 80
BfrI CTTAAG 1 cut(s) 1375
BfuAI ACCTGC 1 cut(s) 362
BfuI GTATCC 3 cut(s) 326, 442, 675
BisI GCNGC 7 cut(s) 75, 80, 795, 1635, 1638, 1804, 2088
BlpI GCTNAGC 1 cut(s) 2549
BlsI GCNGC 7 cut(s) 76, 81, 796, 1636, 1639, 1805, 2089
Bme1390I CCNGG 4 cut(s) 538, 1023, 2268, 2269
Bme18I GGWCC 4 cut(s) 434, 606, 838, 1019
BmeT110I CYCGRG 1 cut(s) 2267
BmgT120I GGNCC 4 cut(s) 434, 606, 838, 1019
BmiI GGNNCC 6 cut(s) 535, 543, 607, 839, 840, 2273
BmrFI CCNGG 4 cut(s) 538, 1023, 2268, 2269
BmsI GCATC 5 cut(s) 571, 1684, 1848, 2108, 2303
BoxI GACNNNNGTC 1 cut(s) 636
BpiI GAAGAC 2 cut(s) 174, 1284
BpmI CTGGAG 1 cut(s) 2120
Bpu1102I GCTNAGC 1 cut(s) 2549
Bpu14I TTCGAA 1 cut(s) 2181
BpuEI CTTGAG 1 cut(s) 2071
BpuMI CCSGG 3 cut(s) 538, 2268, 2269
BsaAI YACGTR 2 cut(s) 273, 1749
BsaJI CCNNGG 3 cut(s) 537, 2267, 2459
BsaWI WCCGGW 1 cut(s) 1532
BsaXI ACNNNNNCTCC 2 cut(s) 949, 979
Bsc4I CCNNNNNNNGG 6 cut(s) 19, 86, 1980, 2227, 2544, 2667
Bse118I RCCGGY 1 cut(s) 1462
Bse1I ACTGG 6 cut(s) 238, 640, 1060, 1196, 1296, 2103
Bse21I CCTNAGG 1 cut(s) 161
Bse3DI GCAATG 2 cut(s) 1969, 2643
BseAI TCCGGA 1 cut(s) 1532
BseBI CCWGG 1 cut(s) 1023
BseDI CCNNGG 3 cut(s) 537, 2267, 2459
BseGI GGATG 9 cut(s) 737, 773, 1077, 1237, 1495, 2123, 2149, 2236, 2626
BseLI CCNNNNNNNGG 6 cut(s) 19, 86, 1980, 2227, 2544, 2667
BseMI GCAATG 2 cut(s) 1969, 2643
BseMII CTCAG 7 cut(s) 580, 1004, 1642, 1746, 1856, 2274, 2502
BseNI ACTGG 6 cut(s) 238, 640, 1060, 1196, 1296, 2103
BseSI GKGCMC 2 cut(s) 791, 1001
BseXI GCAGC 5 cut(s) 86, 781, 1646, 1649, 2074
BsgI GTGCAG 2 cut(s) 1653, 2073
BshFI GGCC 5 cut(s) 159, 230, 571, 1827, 2003
BshNI GGYRCC 1 cut(s) 2271
BsiHKAI GWGCWC 2 cut(s) 483, 1001
BsiHKCI CYCGRG 1 cut(s) 2267
BsiSI CCGG 4 cut(s) 537, 1463, 1533, 2268
BslFI GGGAC 4 cut(s) 17, 476, 851, 970
BslI CCNNNNNNNGG 6 cut(s) 19, 86, 1980, 2227, 2544, 2667
BsmAI GTCTC 2 cut(s) 1732, 2430
BsmFI GGGAC 4 cut(s) 17, 476, 851, 970
BsnI GGCC 5 cut(s) 159, 230, 571, 1827, 2003
BsoBI CYCGRG 1 cut(s) 2267
Bsp119I TTCGAA 1 cut(s) 2181
Bsp1286I GDGCHC 3 cut(s) 483, 791, 1001
Bsp13I TCCGGA 1 cut(s) 1532
Bsp1407I TGTACA 1 cut(s) 508
Bsp1720I GCTNAGC 1 cut(s) 2549
BspACI CCGC 6 cut(s) 80, 625, 1002, 1803, 2126, 2392
BspANI GGCC 5 cut(s) 159, 230, 571, 1827, 2003
BspCNI CTCAG 7 cut(s) 579, 1005, 1641, 1747, 1855, 2275, 2501
BspEI TCCGGA 1 cut(s) 1532
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 6 cut(s) 535, 543, 607, 839, 840, 2273
BspMAI CTGCAG 1 cut(s) 1413
BspMI ACCTGC 1 cut(s) 362
BspPI GGATC 5 cut(s) 1196, 1256, 1390, 2450, 2580
BspT104I TTCGAA 1 cut(s) 2181
BspT107I GGYRCC 1 cut(s) 2271
BspTI CTTAAG 1 cut(s) 1375
BsrBI CCGCTC 1 cut(s) 2392
BsrDI GCAATG 2 cut(s) 1969, 2643
BsrFI RCCGGY 1 cut(s) 1462
BsrGI TGTACA 1 cut(s) 508
BsrI ACTGG 6 cut(s) 238, 640, 1060, 1196, 1296, 2103
BssAI RCCGGY 1 cut(s) 1462
BssECI CCNNGG 3 cut(s) 537, 2267, 2459
Bst2UI CCWGG 1 cut(s) 1023
Bst6I CTCTTC 2 cut(s) 68, 74
BstAFI CTTAAG 1 cut(s) 1375
BstAUI TGTACA 1 cut(s) 508
BstBAI YACGTR 2 cut(s) 273, 1749
BstBI TTCGAA 1 cut(s) 2181
BstC8I GCNNGC 2 cut(s) 2260, 2521
BstENI CCTNNNNNAGG 1 cut(s) 2665
BstF5I GGATG 9 cut(s) 737, 773, 1077, 1237, 1495, 2123, 2149, 2236, 2626
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstMAI GTCTC 2 cut(s) 1732, 2430
BstMWI GCNNNNNNNGC 2 cut(s) 144, 2084
BstNI CCWGG 1 cut(s) 1023
BstNSI RCATGY 3 cut(s) 491, 515, 2523
BstPAI GACNNNNGTC 1 cut(s) 636
BstSCI CCNGG 4 cut(s) 536, 1021, 2266, 2267
BstSFI CTRYAG 4 cut(s) 5, 1243, 1409, 2223
BstSLI GKGCMC 2 cut(s) 791, 1001
BstSNI TACGTA 1 cut(s) 1749
BstV1I GCAGC 5 cut(s) 86, 781, 1646, 1649, 2074
BstV2I GAAGAC 2 cut(s) 174, 1284
BstX2I RGATCY 2 cut(s) 1201, 2572
BstYI RGATCY 2 cut(s) 1201, 2572
Bsu36I CCTNAGG 1 cut(s) 161
BsuI GTATCC 3 cut(s) 326, 442, 675
BsuRI GGCC 5 cut(s) 159, 230, 571, 1827, 2003
BtsCI GGATG 9 cut(s) 737, 773, 1077, 1237, 1495, 2123, 2149, 2236, 2626
BtsIMutI CAGTG 9 cut(s) 231, 459, 603, 633, 852, 1303, 1610, 1636, 1881
BveI ACCTGC 1 cut(s) 362
Cac8I GCNNGC 2 cut(s) 2260, 2521
CciI TCATGA 1 cut(s) 679
CfoI GCGC 1 cut(s) 79
Cfr10I RCCGGY 1 cut(s) 1462
Cfr13I GGNCC 4 cut(s) 434, 606, 838, 1019
Cfr9I CCCGGG 1 cut(s) 2267
CspCI CAANNNNNGTGG 4 cut(s) 698, 733, 1446, 1481
EaeI YGGCCR 3 cut(s) 228, 1825, 2001
Eam1104I CTCTTC 2 cut(s) 68, 74
EarI CTCTTC 2 cut(s) 68, 74
EciI GGCGGA 1 cut(s) 2115
Ecl136II GAGCTC 1 cut(s) 481
Eco105I TACGTA 1 cut(s) 1749
Eco24I GRGCYC 1 cut(s) 483
Eco47I GGWCC 4 cut(s) 434, 606, 838, 1019
Eco53kI GAGCTC 1 cut(s) 481
Eco57I CTGAAG 2 cut(s) 157, 876
Eco81I CCTNAGG 1 cut(s) 161
Eco88I CYCGRG 1 cut(s) 2267
EcoICRI GAGCTC 1 cut(s) 481
EcoNI CCTNNNNNAGG 1 cut(s) 2665
EcoO109I RGGNCCY 2 cut(s) 606, 838
EcoRI GAATTC 2 cut(s) 755, 1112
EcoRII CCWGG 1 cut(s) 1021
EcoT22I ATGCAT 1 cut(s) 2521
EcoT38I GRGCYC 1 cut(s) 483
FalI AAGNNNNNCTT 6 cut(s) 47, 79, 1100, 1132, 2570, 2602
FaqI GGGAC 4 cut(s) 17, 476, 851, 970
FbaI TGATCA 1 cut(s) 363
FblI GTMKAC 1 cut(s) 2365
Fnu4HI GCNGC 7 cut(s) 75, 80, 795, 1635, 1638, 1804, 2088
FokI GGATG 9 cut(s) 724, 760, 1064, 1244, 1502, 2130, 2156, 2243, 2633
FriOI GRGCYC 1 cut(s) 483
Fsp4HI GCNGC 7 cut(s) 75, 80, 795, 1635, 1638, 1804, 2088
FspBI CTAG 3 cut(s) 113, 915, 2379
GlaI GCGC 1 cut(s) 78
GluI GCNGC 7 cut(s) 75, 80, 795, 1635, 1638, 1804, 2088
GsuI CTGGAG 1 cut(s) 2120
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 5 cut(s) 159, 230, 571, 1827, 2003
HapII CCGG 4 cut(s) 537, 1463, 1533, 2268
HhaI GCGC 1 cut(s) 79
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 2 cut(s) 1170, 2366
HindII GTYRAC 2 cut(s) 1170, 2366
HindIII AAGCTT 1 cut(s) 301
HinfI GANTC 3 cut(s) 687, 818, 2557
HpaII CCGG 4 cut(s) 537, 1463, 1533, 2268
HphI GGTGA 2 cut(s) 248, 535
Hpy99I CGWCG 1 cut(s) 782
HpyAV CCTTC 6 cut(s) 1079, 1102, 1301, 1332, 1480, 2462
HpyCH4IV ACGT 3 cut(s) 272, 1172, 1748
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 2084
HpySE526I ACGT 3 cut(s) 272, 1172, 1748
HspAI GCGC 1 cut(s) 77
KflI GGGWCCC 1 cut(s) 838
Kpn2I TCCGGA 1 cut(s) 1532
KpnI GGTACC 1 cut(s) 2275
Ksp22I TGATCA 1 cut(s) 363
LmnI GCTCC 3 cut(s) 539, 903, 970
Lsp1109I GCAGC 5 cut(s) 86, 781, 1646, 1649, 2074
LweI GCATC 5 cut(s) 571, 1684, 1848, 2108, 2303
MaeI CTAG 3 cut(s) 113, 915, 2379
MaeII ACGT 3 cut(s) 272, 1172, 1748
MaeIII GTNAC 6 cut(s) 42, 340, 629, 1208, 1401, 2396
MbiI CCGCTC 1 cut(s) 2392
MboII GAAGA 8 cut(s) 55, 61, 179, 538, 1284, 1567, 1570, 1904
MfeI CAATTG 1 cut(s) 1044
MflI RGATCY 2 cut(s) 1201, 2572
MhlI GDGCHC 3 cut(s) 483, 791, 1001
MlsI TGGCCA 2 cut(s) 230, 2003
MluNI TGGCCA 2 cut(s) 230, 2003
MlyI GAGTC 1 cut(s) 2566
MmeI TCCRAC 6 cut(s) 441, 925, 1631, 1933, 2328, 2555
Mox20I TGGCCA 2 cut(s) 230, 2003
Mph1103I ATGCAT 1 cut(s) 2521
MroI TCCGGA 1 cut(s) 1532
MroXI GAANNNNTTC 2 cut(s) 1112, 1540
MscI TGGCCA 2 cut(s) 230, 2003
MslI CAYNNNNRTG 2 cut(s) 2142, 2648
Msp20I TGGCCA 2 cut(s) 230, 2003
MspA1I CMGCKG 2 cut(s) 647, 794
MspCI CTTAAG 1 cut(s) 1375
MspI CCGG 4 cut(s) 537, 1463, 1533, 2268
MspR9I CCNGG 4 cut(s) 538, 1023, 2268, 2269
MunI CAATTG 1 cut(s) 1044
MvaI CCWGG 1 cut(s) 1023
MwoI GCNNNNNNNGC 2 cut(s) 144, 2084
NciI CCSGG 3 cut(s) 538, 2268, 2269
NlaIV GGNNCC 6 cut(s) 535, 543, 607, 839, 840, 2273
NmuCI GTSAC 3 cut(s) 42, 629, 1401
NsiI ATGCAT 1 cut(s) 2521
NspI RCATGY 3 cut(s) 491, 515, 2523
NspV TTCGAA 1 cut(s) 2181
PaeI GCATGC 1 cut(s) 2523
PagI TCATGA 1 cut(s) 679
PciI ACATGT 2 cut(s) 487, 511
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 278
PdmI GAANNNNTTC 2 cut(s) 1112, 1540
PfeI GAWTC 2 cut(s) 687, 818
PflMI CCANNNNNTGG 1 cut(s) 19
PkrI GCNGC 7 cut(s) 76, 81, 796, 1636, 1639, 1805, 2089
PleI GAGTC 1 cut(s) 2565
PpsI GAGTC 1 cut(s) 2565
Ppu21I YACGTR 2 cut(s) 273, 1749
PpuMI RGGWCCY 2 cut(s) 606, 838
PscI ACATGT 2 cut(s) 487, 511
PshAI GACNNNNGTC 1 cut(s) 636
Psp124BI GAGCTC 1 cut(s) 483
Psp5II RGGWCCY 2 cut(s) 606, 838
Psp6I CCWGG 1 cut(s) 1021
PspGI CCWGG 1 cut(s) 1021
PspN4I GGNNCC 6 cut(s) 535, 543, 607, 839, 840, 2273
PspPI GGNCC 4 cut(s) 434, 606, 838, 1019
PspPPI RGGWCCY 2 cut(s) 606, 838
PstI CTGCAG 1 cut(s) 1413
PsuI RGATCY 2 cut(s) 1201, 2572
PvuII CAGCTG 2 cut(s) 647, 794
RseI CAYNNNNRTG 2 cut(s) 2142, 2648
SacI GAGCTC 1 cut(s) 483
SalI GTCGAC 1 cut(s) 2364
SatI GCNGC 7 cut(s) 75, 80, 795, 1635, 1638, 1804, 2088
Sau96I GGNCC 4 cut(s) 434, 606, 838, 1019
SchI GAGTC 1 cut(s) 2566
ScrFI CCNGG 4 cut(s) 538, 1023, 2268, 2269
SduI GDGCHC 3 cut(s) 483, 791, 1001
SfaNI GCATC 5 cut(s) 571, 1684, 1848, 2108, 2303
SfcI CTRYAG 4 cut(s) 5, 1243, 1409, 2223
SfuI TTCGAA 1 cut(s) 2181
SinI GGWCC 4 cut(s) 434, 606, 838, 1019
SmaI CCCGGG 1 cut(s) 2269
SmiMI CAYNNNNRTG 2 cut(s) 2142, 2648
SmlI CTYRAG 2 cut(s) 1375, 2050
SmoI CTYRAG 2 cut(s) 1375, 2050
SnaBI TACGTA 1 cut(s) 1749
SphI GCATGC 1 cut(s) 2523
SsiI CCGC 6 cut(s) 80, 625, 1002, 1803, 2126, 2392
SspI AATATT 4 cut(s) 17, 829, 993, 1180
SspMI CTAG 3 cut(s) 113, 915, 2379
SstI GAGCTC 1 cut(s) 483
StyD4I CCNGG 4 cut(s) 536, 1021, 2266, 2267
TaiI ACGT 3 cut(s) 275, 1175, 1751
TaqI TCGA 5 cut(s) 477, 685, 759, 2181, 2365
TatI WGTACW 5 cut(s) 508, 859, 1762, 1995, 2341
TauI GCSGC 2 cut(s) 82, 1806
TfiI GAWTC 2 cut(s) 687, 818
TscAI CASTG 9 cut(s) 238, 459, 603, 640, 859, 1303, 1617, 1636, 1881
TseFI GTSAC 3 cut(s) 42, 629, 1401
TseI GCWGC 5 cut(s) 74, 794, 1634, 1637, 2087
Tsp45I GTSAC 3 cut(s) 42, 629, 1401
TspDTI ATGAA 9 cut(s) 68, 107, 130, 420, 1916, 1983, 2187, 2410, 2637
TspGWI ACGGA 4 cut(s) 61, 256, 766, 954
TspMI CCCGGG 1 cut(s) 2267
TspRI CASTG 9 cut(s) 238, 459, 603, 640, 859, 1303, 1617, 1636, 1881
Van91I CCANNNNNTGG 1 cut(s) 19
Vha464I CTTAAG 1 cut(s) 1375
VneI GTGCAC 1 cut(s) 997
VpaK11BI GGWCC 4 cut(s) 434, 606, 838, 1019
XagI CCTNNNNNAGG 1 cut(s) 2665
XapI RAATTY 8 cut(s) 755, 1112, 1395, 1429, 1936, 2177, 2473, 2638
XbaI TCTAGA 2 cut(s) 914, 2378
XceI RCATGY 3 cut(s) 491, 515, 2523
XmaI CCCGGG 1 cut(s) 2267
XmiI GTMKAC 1 cut(s) 2365
XmnI GAANNNNTTC 2 cut(s) 1112, 1540
XspI CTAG 3 cut(s) 113, 915, 2379
Zsp2I ATGCAT 1 cut(s) 2521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.