MD17G1179300.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000083
Physical Location & Seq
Reverse (-)
20579071 .. 20583160
4090 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1179300.v1.1.491

Sequence Viewer

Length: 2649 bp
ATGGGAATGTTGAAAAACCTTCGATTTGTATTCCTTTGTTGTTTTTCTCTTATACTTCTAGTTCATGCACAAGATGATCAATCAGGTTTCATCAGCTTGGACTGTGGACAGCCATCAAATTCTAGCTACTCCGAGTCAACAACCGGCATTGACTACATTTCAGATGCAGCCTTCATAAGCACTGGTGTGAGCAAAAGCGTCGCACCTGCATACAAAGCTACTCATCAGCTACAGGCAGCTTATGTCAGGAGCTTTCCTCGGGGGGTCAAGAACTGTTACAAAGTAAACATCACAAAGAACACCAAGTATTTAATCCGAGCTGCTTTCCTGTATGGTAATTATGACGGCCTAAACAAGCTGCCAAAGTTTGATCTTGTCATCGGAGCTAGTTACTGGGATTCAGTGGCATTTATTGATGCATCTTTAAGTACCATCAAAGAAGTTGTTCACATTCCCACGTTAGACTACATAAATGTGTGTTTACTCAACAAGGGAACCGGAGCACCATTCATTTCATCATTGGAATTAAGGCCTTTGAAAAACACTACTTATCTGACTCCAACGGGTTCATTGGCACTCTCCTTTCGATTAGATGTCGGTTCAACAGTTAATCAAACATACAGGTATCCCTATGATGCTTTGGATCGGATTTGGCAACCCTTCAACTTCAATAAGTGGACGCAGTTAGCTACCTCGCTTACCGTTGATGCTCAAAGTCACAATGATTACCATGTACCATCTTTCGTAATGAGATCTGCCAGCACGCCTCTAGATCCTAGTGATACGATGGACTTCAATTGGAATGCCCCGGATACAAGTACTCAATACTATATCTTCATTCACTTTGCTGAATTCCAGCTGCTTAAAGCCAACCAGTCCAGATCATTCAATATCACGTTAAATGGAGATCATTGGTTTGGACCTTTTGCCCCGGCTTATTTATCCACAATCACTGTGTTTAGCCCCTCAGCCTTAACCGGAGGCAATTATAGTTTCTCACTTGTTCAAACGGAGAATTCAACTCTCCCACCAATTCTCAACGCCATGGAGGTCTACACATTGATAGATTTATCACAATCTGAAACCGACACAGATGATGCGTCTGCCATCACAAACATAAAGTCAACATATGGAGTGGACAAAGATTGGCAAGGAGACCCGTGTACTCCTGAAGGCTACATGTGGGAAGGTCTAAATTGTACCTTCAACGGTTCCCCCAGAATTATATCCTTGGAGCTGTCCTCGAGTGGATTGACCGGGGAGATAACTTCATATATATTTGACCTCGCCATGTTACAATCTTTGGATTTATCAAACAACAGCTTAACTGGATCAATACCTGAATTTCTGTCTAAAATGCCAAACCTGAAAGTCCTTAATTTAGGAAGAAACAAGCTCAACGGTTCAGTTCCAGCCGACCTCATTGAAAGATCGAAAAGTGGTTCGCTATCATTGAGTGTTGGAGAAAATGCAGGCCTATGTGCAGAAAATTCATGCAAAAAGGAGGAGACAGAGAAAAAGAAGAACATAATTGTTCCAATAGGAGCAGCAATTGGTGGATTTTTTATTCTTGTGGTACTTGTAGTGGCTATATTGATGTGGCTAAAAAGAGGAAGGAAACAACAAGTTGTAACAGCAGTTTCACATCAACCCAACAATCAAGTTGATTCATTCGAGTCCAAAAAACGACAGTTTACATACTTGGACGTCCTGCAGATTACAAACAACTTTCAGACAATTATTGGCAGAGGTGGATTCGGAAAAGTTTACCATGGTTTAGTCGATGATACTCAAGTAGCTGTAAAGATGCTTTCTCCAACATCAGGTCAAGGATACCAACAGTTTCAAGCAGAGGTCAAACTTCTAATCAGAGTTCATCACAGAAACTTGACAAGTCTTGTGGGATACTGCAACGAAGGAACCAACATGGCGCTCATCTATGAATTTATGGCCAATGGAAATTTGGACTCGCATCTTCTAGGTGAGAATAATAATGCAAATGTTTTGACTTGGGAAGGTAGACTCCAAATAGCAACAGATGCAGCACAAGGATTGGAGTACCTTCACAACGGTTGCAAGCCACCCATAGTCCACAGAGATGTGAAGACAACAAATATTTTGCTAGCCGAAAATTTCCAAGCAAAATTAGCTGATTTTGGTCTGTCCAGAATTTTCCCAACTGATGGACGCACTCATATGTCTACTGCTGTAGCTGGAACTCCGGGATACCTTGATCCCGAGTACCACACAACAGGTTGGCTCAATGAGAAGAGTGATGTTTATAGCTTTGGAGTTGTTCTGTTGGAGATCATCACTGGCCGCCCTGCGATATCAAGAACACAGGAGAAGGTTCATGTAAGTCAATGGGTTAGCTCCATGCTTGCTATGGGGGATATCAAAACTATTCTAGATCCGAGATTACGTGGAGATTACGAAATCAACTCTGCCTGGAAGGCTGTTGAACTAGCAATGAATTGTGTGTCAGATACATCCAACAAAAGGGCAAACATGAGTGAGGTAGTGAGAGGGCTTAAGGATTGTTTGGAAGCTGAGTTGGCTCGAACGAACGTCAACCGTGTGACTGAATCAACAGATTCAGTTATTTACTCTATGAATGTGACTACGGAACTTAGTCCTTTGGCAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

883

Amino Acids

97.48

Weight (kDa)

5.91

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 33 - 353 1.4e-118 Malectin-like domain
LRR_8 PF13855 410 - 466 1.2e-06 Leucine rich repeat
LRR_8 PF13855 432 - 473 1.8e-06 Leucine rich repeat
LRR_4 PF12799 432 - 466 3.6e-08 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 577 - 842 1.6e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 578 - 841 1.9e-41 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000083)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07560 AT1G51790 AT1G51790 AT1G51790 AT1G51800 AT1G51805 AT1G51805 AT1G51810 AT1G51820 AT1G51820 AT1G51830 AT1G51830 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51850 AT1G51850 AT1G51860 AT1G51860 AT1G51870 AT1G51870 AT1G51880 AT1G51880 AT1G51880 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51910 AT2G04300 AT2G04300 AT2G28960 AT2G28960 AT2G28970 AT2G28970 AT2G28990 AT2G28990 AT2G29000 AT3G21340 AT3G46350 AT5G59616 AT5G59650 AT5G59650 AT5G59660 AT5G59660 AT5G59660 AT5G59670 AT5G59670 AT5G59680
fragaria_vesca FvH4_2g03270 FvH4_3g21300 FvH4_6g32660 FvH4_6g32660 FvH4_6g32670 FvH4_6g32670 FvH4_6g32690 FvH4_6g32690 FvH4_6g32691 FvH4_6g32700 FvH4_6g32740 FvH4_6g32750 FvH4_6g33210 FvH4_6g33210 FvH4_7g24270 FvH4_7g24280 FvH4_7g24291 FvH4_7g24300
malus_domestica MD09G1198400.v1.1 MD09G1198600.v1.1 MD09G1199100.v1.1 MD09G1199200.v1.1 MD09G1199300.v1.1 MD09G1199500.v1.1 MD09G1199600.v1.1 MD17G1179300.v1.1 MD17G1180200.v1.1 MD17G1266000.v1.1
prunus_persica Prupe.1G438900_v2.0.a1 Prupe.1G439000_v2.0.a1 Prupe.1G439100_v2.0.a1 Prupe.3G019400_v2.0.a1 Prupe.3G021900_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G055100_v2.0.a1 Prupe.3G055400_v2.0.a1 Prupe.3G055500_v2.0.a1 Prupe.3G055600_v2.0.a1 Prupe.3G055700_v2.0.a1 Prupe.3G055800_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056100_v2.0.a1 Prupe.3G209600_v2.0.a1 Prupe.3G209700_v2.0.a1 Prupe.6G291100_v2.0.a1 Prupe.7G208900_v2.0.a1 Prupe.8G038300_v2.0.a1
pyrus_communis pycom09g11570 pycom09g11580 pycom09g11610 pycom09g11630 pycom09g11640 pycom17g18840 pycom17g18910 pycom17g18930 pycom17g18940
rosa_chinensis RchiOBHm_Chr1g0317521 RchiOBHm_Chr1g0317541 RchiOBHm_Chr2g0123351 RchiOBHm_Chr2g0141181 RchiOBHm_Chr2g0141191 RchiOBHm_Chr2g0141211 RchiOBHm_Chr2g0141221 RchiOBHm_Chr2g0141261 RchiOBHm_Chr2g0141271 RchiOBHm_Chr2g0141311 RchiOBHm_Chr2g0141331 RchiOBHm_Chr2g0141371 RchiOBHm_Chr2g0141391 RchiOBHm_Chr2g0141401 RchiOBHm_Chr2g0141431 RchiOBHm_Chr2g0141481 RchiOBHm_Chr2g0141491 RchiOBHm_Chr2g0141591 RchiOBHm_Chr2g0141601 RchiOBHm_Chr2g0141611 RchiOBHm_Chr2g0141631 RchiOBHm_Chr2g0141641 RchiOBHm_Chr2g0141661 RchiOBHm_Chr2g0141681 RchiOBHm_Chr2g0141701 RchiOBHm_Chr2g0141781 RchiOBHm_Chr2g0141811 RchiOBHm_Chr2g0141821 RchiOBHm_Chr3g0479311 RchiOBHm_Chr3g0495751 RchiOBHm_Chr4g0404741 RchiOBHm_Chr4g0410341 RchiOBHm_Chr4g0418371 RchiOBHm_Chr4g0438651 RchiOBHm_Chr5g0036411 RchiOBHm_Chr5g0036491 RchiOBHm_Chr5g0042391 RchiOBHm_Chr6g0279871 RchiOBHm_Chr7g0192921 RchiOBHm_Chr7g0214901 RchiOBHm_Chr7g0234931
rosa_laevigata RLG00000003432 RLG00000008806 RLG00000013062 RLG00000019514 RLG00000019923 RLG00000019924 RLG00000019925 RLG00000019930 RLG00000019931 RLG00000019934 RLG00000019937 RLG00000019938 RLG00000033697
rosa_multiflora Rmu_co8002302.1_g000001 Rmu_sc0000147.1_g000031 Rmu_sc0000463.1_g000005 Rmu_sc0000712.1_g000003 Rmu_sc0001483.1_g000017 Rmu_sc0001554.1_g000002 Rmu_sc0001554.1_g000005 Rmu_sc0001554.1_g000008 Rmu_sc0001981.1_g000017 Rmu_sc0002040.1_g000056 Rmu_sc0002755.1_g000018 Rmu_sc0002804.1_g000016 Rmu_sc0002928.1_g000015 Rmu_sc0003040.1_g000008 Rmu_sc0003040.1_g000018 Rmu_sc0004512.1_g000001 Rmu_sc0004512.1_g000004 Rmu_sc0005507.1_g000001 Rmu_sc0005507.1_g000009 Rmu_sc0005507.1_g000033 Rmu_sc0005507.1_g000037 Rmu_sc0005507.1_g000043 Rmu_sc0005507.1_g000047 Rmu_sc0006318.1_g000004 Rmu_sc0006318.1_g000014 Rmu_sc0006318.1_g000021 Rmu_sc0006556.1_g000001 Rmu_sc0007208.1_g000002 Rmu_sc0007208.1_g000003 Rmu_sc0007208.1_g000006 Rmu_sc0007208.1_g000008
rosa_roxburghii Rroxscaffold_1G00044430 Rroxscaffold_2G00103420 Rroxscaffold_2G00103470 Rroxscaffold_2G00103490 Rroxscaffold_2G00103500 Rroxscaffold_2G00103520 Rroxscaffold_2G00103530 Rroxscaffold_2G00103540 Rroxscaffold_2G00103550 Rroxscaffold_2G00103570 Rroxscaffold_2G00103770 Rroxscaffold_2G00103780 Rroxscaffold_2G00103800 Rroxscaffold_2G00103820 Rroxscaffold_2G00103860 Rroxscaffold_2G00103890 Rroxscaffold_2G00103930 Rroxscaffold_2G00103940 Rroxscaffold_2G00104010 Rroxscaffold_2G00104030 Rroxscaffold_2G00104050 Rroxscaffold_2G00104060 Rroxscaffold_2G00104070 Rroxscaffold_2G00104090 Rroxscaffold_2G00104100 Rroxscaffold_2G00104120 Rroxscaffold_2G00104130 Rroxscaffold_4G00331710
rosa_rugosa Rorug02G0367500 Rorug02G0367500 Rorug02G0367600 Rorug02G0367800 Rorug02G0367800 Rorug02G0367900 Rorug02G0368100 Rorug02G0368100 Rorug02G0368100 Rorug02G0368200 Rorug02G0368700 Rorug02G0368900 Rorug05G0155600 Rorug05G0155700 Rorug05G0155800 Rorug05G0156700 Rorug05G0157300
rosa_samantha Rh1AG028000 Rh2AG418700 Rh2AG418900 Rh2AG419000 Rh2AG419100 Rh2AG419400 Rh2AG419600 Rh2AG419800 Rh2AG420400 Rh2AG421000 Rh2BG428500 Rh2BG428600 Rh2BG428800 Rh2BG428900 Rh2BG429300 Rh2BG429400 Rh2BG429700 Rh2BG429900 Rh2BG430000 Rh2BG430700 Rh2CG405100 Rh2CG405200 Rh2CG405300 Rh2CG405500 Rh2CG405600 Rh2CG405700 Rh2CG406000 Rh2CG406100 Rh2CG406300 Rh2CG406600 Rh2CG406700 Rh2CG407500 Rh2CG408100 Rh2DG438500 Rh3DG123900 Rh3DG124000 Rh5BG249700 Rh5BG482500 Rh5CG281500 Rh5CG507300 Rh7DG133300
rosa_wichuraiana Rw0G019720 Rw0G019730 Rw0G019750 Rw0G023010 Rw2G034340 Rw2G034350 Rw2G034370 Rw2G034380 Rw2G034400 Rw2G034430 Rw2G034470 Rw2G034480 Rw2G034510 Rw5G022890 Rw5G026410 Rw5G032710 Rw5G042850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 214
AatII GACGTC 1 cut(s) 1710
Acc36I ACCTGC 1 cut(s) 214
AccB7I CCANNNNNTGG 1 cut(s) 2183
AccI GTMKAC 3 cut(s) 1053, 2020, 2201
AciI CCGC 1 cut(s) 2320
AclWI GGATC 5 cut(s) 651, 767, 1339, 2228, 2405
AcoI YGGCCR 2 cut(s) 1950, 2317
AcsI RAATTY 9 cut(s) 118, 851, 1015, 1343, 1489, 1943, 1960, 2131, 2169
AcuI CTGAAG 1 cut(s) 1191
AcyI GRCGYC 1 cut(s) 1707
AfaI GTAC 8 cut(s) 430, 735, 820, 1165, 1201, 1578, 2060, 2243
AfiI CCNNNNNNNGG 3 cut(s) 1823, 2183, 2499
AflII CTTAAG 1 cut(s) 2531
AflIII ACRYGT 1 cut(s) 1179
AjnI CCWGG 1 cut(s) 2447
AleI CACNNNNGTG 1 cut(s) 185
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 2 cut(s) 1149, 1502
AlwI GGATC 5 cut(s) 651, 767, 1339, 2228, 2405
Ama87I CYCGRG 3 cut(s) 258, 1243, 2237
AoxI GGCC 5 cut(s) 346, 530, 1474, 1950, 2317
ApeKI GCWGC 7 cut(s) 167, 236, 320, 358, 859, 1547, 2042
ApoI RAATTY 9 cut(s) 118, 851, 1015, 1343, 1489, 1943, 1960, 2131, 2169
ArsI GACNNNNNNTTYG 2 cut(s) 1106, 1138
Asp700I GAANNNNTTC 1 cut(s) 444
AspLEI GCGC 1 cut(s) 1933
AspS9I GGNCC 1 cut(s) 920
AsuC2I CCSGG 4 cut(s) 809, 932, 1258, 2223
AsuHPI GGTGA 1 cut(s) 1994
AsuNHI GCTAGC 1 cut(s) 2122
AvaI CYCGRG 3 cut(s) 258, 1243, 2237
AvaII GGWCC 1 cut(s) 920
BaeI ACNNNNGTAYC 2 cut(s) 1779, 1812
BalI TGGCCA 1 cut(s) 1952
BbsI GAAGAC 1 cut(s) 2111
Bbv12I GWGCWC 1 cut(s) 505
BbvCI CCTCAGC 1 cut(s) 967
BbvI GCAGC 7 cut(s) 179, 248, 307, 345, 846, 1559, 2054
BccI CCATC 6 cut(s) 121, 440, 745, 781, 1115, 2177
BceAI ACGGC 1 cut(s) 361
BcgI CGANNNNNNTGC 2 cut(s) 181, 215
BciT130I CCWGG 1 cut(s) 2449
BciVI GTATCC 5 cut(s) 636, 805, 1826, 1898, 2219
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 4 cut(s) 809, 932, 1258, 2223
BcoDI GTCTC 2 cut(s) 1149, 1502
BfaI CTAG 9 cut(s) 59, 123, 387, 770, 777, 1979, 2123, 2408, 2465
BfmI CTRYAG 3 cut(s) 230, 1712, 2208
BfoI RGCGCY 1 cut(s) 1934
BfrI CTTAAG 1 cut(s) 2531
BfuAI ACCTGC 1 cut(s) 214
BfuI GTATCC 5 cut(s) 636, 805, 1826, 1898, 2219
BglI GCCNNNNNGGC 1 cut(s) 2453
BglII AGATCT 1 cut(s) 752
BisI GCNGC 8 cut(s) 168, 237, 321, 359, 860, 1548, 2043, 2320
BlsI GCNGC 8 cut(s) 169, 238, 322, 360, 861, 1549, 2044, 2321
BmcAI AGTACT 1 cut(s) 820
Bme1390I CCNGG 5 cut(s) 809, 932, 1258, 2223, 2449
Bme18I GGWCC 1 cut(s) 920
BmeT110I CYCGRG 3 cut(s) 258, 1243, 2237
BmgT120I GGNCC 1 cut(s) 920
BmiI GGNNCC 3 cut(s) 496, 1213, 1921
BmrFI CCNGG 5 cut(s) 809, 932, 1258, 2223, 2449
BmrI ACTGGG 1 cut(s) 403
BmsI GCATC 9 cut(s) 154, 406, 428, 625, 697, 1087, 1797, 1981, 2029
BmtI GCTAGC 1 cut(s) 2126
BmuI ACTGGG 1 cut(s) 403
BpiI GAAGAC 1 cut(s) 2111
BplI GAGNNNNNCTC 4 cut(s) 241, 273, 1226, 1258
Bpu10I CCTNAGC 1 cut(s) 967
BpuEI CTTGAG 1 cut(s) 1776
BpuMI CCSGG 4 cut(s) 809, 932, 1258, 2223
BsaAI YACGTR 1 cut(s) 2423
BsaHI GRCGYC 1 cut(s) 1707
BsaI GGTCTC 1 cut(s) 1149
BsaJI CCNNGG 7 cut(s) 257, 807, 930, 1044, 1230, 1257, 1771
BsaWI WCCGGW 2 cut(s) 497, 977
Bsc4I CCNNNNNNNGG 3 cut(s) 1823, 2183, 2499
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 5 cut(s) 187, 398, 874, 1333, 2320
Bse3DI GCAATG 1 cut(s) 2475
BseBI CCWGG 1 cut(s) 2449
BseDI CCNNGG 7 cut(s) 257, 807, 930, 1044, 1230, 1257, 1771
BseGI GGATG 1 cut(s) 2489
BseLI CCNNNNNNNGG 3 cut(s) 1823, 2183, 2499
BseMI GCAATG 1 cut(s) 2475
BseMII CTCAG 2 cut(s) 981, 2541
BseNI ACTGG 5 cut(s) 187, 398, 874, 1333, 2320
BseRI GAGGAG 1 cut(s) 1520
BseXI GCAGC 7 cut(s) 179, 248, 307, 345, 846, 1559, 2054
BsgI GTGCAG 1 cut(s) 1503
BshFI GGCC 5 cut(s) 348, 532, 1476, 1952, 2319
BsiHKAI GWGCWC 1 cut(s) 505
BsiHKCI CYCGRG 3 cut(s) 258, 1243, 2237
BsiSI CCGG 7 cut(s) 144, 498, 809, 932, 978, 1257, 2222
BslI CCNNNNNNNGG 3 cut(s) 1823, 2183, 2499
BsmAI GTCTC 2 cut(s) 1149, 1502
BsmI GAATGC 1 cut(s) 808
BsnI GGCC 5 cut(s) 348, 532, 1476, 1952, 2319
Bso31I GGTCTC 1 cut(s) 1149
BsoBI CYCGRG 3 cut(s) 258, 1243, 2237
Bsp1286I GDGCHC 1 cut(s) 505
Bsp19I CCATGG 2 cut(s) 1044, 1771
BspACI CCGC 1 cut(s) 2320
BspANI GGCC 5 cut(s) 348, 532, 1476, 1952, 2319
BspCNI CTCAG 2 cut(s) 980, 2542
BspLI GGNNCC 3 cut(s) 496, 1213, 1921
BspMAI CTGCAG 1 cut(s) 1716
BspMI ACCTGC 1 cut(s) 214
BspOI GCTAGC 1 cut(s) 2126
BspPI GGATC 5 cut(s) 651, 767, 1339, 2228, 2405
BspTI CTTAAG 1 cut(s) 2531
BspTNI GGTCTC 1 cut(s) 1149
BsrDI GCAATG 1 cut(s) 2475
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 5 cut(s) 187, 398, 874, 1333, 2320
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 7 cut(s) 257, 807, 930, 1044, 1230, 1257, 1771
BssNI GRCGYC 1 cut(s) 1707
BssT1I CCWWGG 3 cut(s) 1044, 1230, 1771
Bst2UI CCWGG 1 cut(s) 2449
Bst6I CTCTTC 1 cut(s) 2264
BstACI GRCGYC 1 cut(s) 1707
BstAFI CTTAAG 1 cut(s) 2531
BstAPI GCANNNNNTGC 1 cut(s) 2039
BstBAI YACGTR 1 cut(s) 2423
BstC8I GCNNGC 6 cut(s) 760, 764, 1474, 2078, 2124, 2382
BstDEI CTNAG 3 cut(s) 967, 2550, 2630
BstDSI CCRYGG 2 cut(s) 1044, 1771
BstF5I GGATG 1 cut(s) 2489
BstH2I RGCGCY 1 cut(s) 1934
BstHHI GCGC 1 cut(s) 1933
BstMAI GTCTC 2 cut(s) 1149, 1502
BstMWI GCNNNNNNNGC 5 cut(s) 215, 2039, 2147, 2453, 2555
BstNI CCWGG 1 cut(s) 2449
BstNSI RCATGY 1 cut(s) 1183
BstSCI CCNGG 5 cut(s) 807, 930, 1256, 2221, 2447
BstSFI CTRYAG 3 cut(s) 230, 1712, 2208
BstV1I GCAGC 7 cut(s) 179, 248, 307, 345, 846, 1559, 2054
BstV2I GAAGAC 1 cut(s) 2111
BstX2I RGATCY 3 cut(s) 752, 772, 2410
BstYI RGATCY 3 cut(s) 752, 772, 2410
BsuI GTATCC 5 cut(s) 636, 805, 1826, 1898, 2219
BsuRI GGCC 5 cut(s) 348, 532, 1476, 1952, 2319
BtgI CCRYGG 2 cut(s) 1044, 1771
BtsCI GGATG 1 cut(s) 2489
BtsIMutI CAGTG 4 cut(s) 180, 408, 951, 2313
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 6 cut(s) 760, 764, 1474, 2078, 2124, 2382
CfoI GCGC 1 cut(s) 1933
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 920
CseI GACGC 4 cut(s) 187, 688, 1089, 2196
Csp6I GTAC 8 cut(s) 429, 734, 819, 1164, 1200, 1577, 2059, 2242
CspCI CAANNNNNGTGG 2 cut(s) 1881, 1916
CviQI GTAC 8 cut(s) 429, 734, 819, 1164, 1200, 1577, 2059, 2242
DdeI CTNAG 3 cut(s) 967, 2550, 2630
EaeI YGGCCR 2 cut(s) 1950, 2317
Eam1104I CTCTTC 1 cut(s) 2264
EarI CTCTTC 1 cut(s) 2264
Eco130I CCWWGG 3 cut(s) 1044, 1230, 1771
Eco147I AGGCCT 2 cut(s) 532, 1476
Eco31I GGTCTC 1 cut(s) 1149
Eco32I GATATC 2 cut(s) 2331, 2395
Eco47I GGWCC 1 cut(s) 920
Eco57I CTGAAG 1 cut(s) 1191
Eco88I CYCGRG 3 cut(s) 258, 1243, 2237
EcoRI GAATTC 2 cut(s) 851, 1015
EcoRII CCWGG 1 cut(s) 2447
EcoRV GATATC 2 cut(s) 2331, 2395
EcoT14I CCWWGG 3 cut(s) 1044, 1230, 1771
EcoT22I ATGCAT 1 cut(s) 421
ErhI CCWWGG 3 cut(s) 1044, 1230, 1771
FauNDI CATATG 2 cut(s) 1129, 2196
FbaI TGATCA 1 cut(s) 76
FblI GTMKAC 3 cut(s) 1053, 2020, 2201
Fnu4HI GCNGC 8 cut(s) 168, 237, 321, 359, 860, 1548, 2043, 2320
FokI GGATG 1 cut(s) 2476
Fsp4HI GCNGC 8 cut(s) 168, 237, 321, 359, 860, 1548, 2043, 2320
FspBI CTAG 9 cut(s) 59, 123, 387, 770, 777, 1979, 2123, 2408, 2465
GlaI GCGC 1 cut(s) 1932
GluI GCNGC 8 cut(s) 168, 237, 321, 359, 860, 1548, 2043, 2320
HaeII RGCGCY 1 cut(s) 1934
HaeIII GGCC 5 cut(s) 348, 532, 1476, 1952, 2319
HapII CCGG 7 cut(s) 144, 498, 809, 932, 978, 1257, 2222
HgaI GACGC 4 cut(s) 187, 688, 1089, 2196
HhaI GCGC 1 cut(s) 1933
Hin1I GRCGYC 1 cut(s) 1707
Hin6I GCGC 1 cut(s) 1931
HinP1I GCGC 1 cut(s) 1931
HincII GTYRAC 3 cut(s) 138, 1125, 2572
HindII GTYRAC 3 cut(s) 138, 1125, 2572
HpaII CCGG 7 cut(s) 144, 498, 809, 932, 978, 1257, 2222
HphI GGTGA 1 cut(s) 1994
Hpy188III TCNNGA 9 cut(s) 247, 268, 770, 879, 1169, 2166, 2237, 2334, 2408
Hpy99I CGWCG 1 cut(s) 203
HpyCH4IV ACGT 5 cut(s) 458, 896, 1707, 2422, 2568
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 2039, 2147, 2453, 2555
HpyF3I CTNAG 3 cut(s) 967, 2550, 2630
HpySE526I ACGT 5 cut(s) 458, 896, 1707, 2422, 2568
Hsp92I GRCGYC 1 cut(s) 1707
HspAI GCGC 1 cut(s) 1931
Ksp22I TGATCA 1 cut(s) 76
LmnI GCTCC 6 cut(s) 249, 383, 500, 1234, 1544, 2378
Lsp1109I GCAGC 7 cut(s) 179, 248, 307, 345, 846, 1559, 2054
LweI GCATC 9 cut(s) 154, 406, 428, 625, 697, 1087, 1797, 1981, 2029
MaeI CTAG 9 cut(s) 59, 123, 387, 770, 777, 1979, 2123, 2408, 2465
MaeII ACGT 5 cut(s) 458, 896, 1707, 2422, 2568
MaeIII GTNAC 7 cut(s) 275, 389, 716, 1293, 1630, 2578, 2617
MboII GAAGA 6 cut(s) 826, 1398, 1534, 1967, 2116, 2281
MfeI CAATTG 2 cut(s) 796, 1551
MflI RGATCY 3 cut(s) 752, 772, 2410
MhlI GDGCHC 1 cut(s) 505
MlsI TGGCCA 1 cut(s) 1952
MluNI TGGCCA 1 cut(s) 1952
MlyI GAGTC 5 cut(s) 143, 550, 1685, 1961, 2016
MmeI TCCRAC 5 cut(s) 584, 1441, 1841, 2283, 2517
Mox20I TGGCCA 1 cut(s) 1952
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 1 cut(s) 444
MscI TGGCCA 1 cut(s) 1952
MslI CAYNNNNRTG 4 cut(s) 185, 473, 2097, 2195
Msp20I TGGCCA 1 cut(s) 1952
MspA1I CMGCKG 1 cut(s) 859
MspCI CTTAAG 1 cut(s) 2531
MspI CCGG 7 cut(s) 144, 498, 809, 932, 978, 1257, 2222
MspR9I CCNGG 5 cut(s) 809, 932, 1258, 2223, 2449
MunI CAATTG 2 cut(s) 796, 1551
Mva1269I GAATGC 1 cut(s) 808
MvaI CCWGG 1 cut(s) 2449
MwoI GCNNNNNNNGC 5 cut(s) 215, 2039, 2147, 2453, 2555
NciI CCSGG 4 cut(s) 809, 932, 1258, 2223
NcoI CCATGG 2 cut(s) 1044, 1771
NdeI CATATG 2 cut(s) 1129, 2196
NheI GCTAGC 1 cut(s) 2122
NlaIV GGNNCC 3 cut(s) 496, 1213, 1921
NmuCI GTSAC 3 cut(s) 716, 2578, 2617
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 1 cut(s) 1183
OliI CACNNNNGTG 1 cut(s) 185
PaeR7I CTCGAG 1 cut(s) 1243
PaqCI CACCTGC 1 cut(s) 214
PceI AGGCCT 2 cut(s) 532, 1476
PciI ACATGT 1 cut(s) 1179
PctI GAATGC 1 cut(s) 808
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 5 cut(s) 398, 1667, 1755, 2585, 2594
PflMI CCANNNNNTGG 1 cut(s) 2183
PfoI TCCNGGA 1 cut(s) 2221
PkrI GCNGC 8 cut(s) 169, 238, 322, 360, 861, 1549, 2044, 2321
PleI GAGTC 5 cut(s) 142, 550, 1684, 1961, 2016
PpsI GAGTC 5 cut(s) 142, 550, 1684, 1961, 2016
Ppu21I YACGTR 1 cut(s) 2423
PscI ACATGT 1 cut(s) 1179
Psp6I CCWGG 1 cut(s) 2447
PspGI CCWGG 1 cut(s) 2447
PspN4I GGNNCC 3 cut(s) 496, 1213, 1921
PspPI GGNCC 1 cut(s) 920
PspXI VCTCGAGB 1 cut(s) 1243
PstI CTGCAG 1 cut(s) 1716
PsuI RGATCY 3 cut(s) 752, 772, 2410
PvuII CAGCTG 1 cut(s) 859
RsaI GTAC 8 cut(s) 430, 735, 820, 1165, 1201, 1578, 2060, 2243
RsaNI GTAC 8 cut(s) 429, 734, 819, 1164, 1200, 1577, 2059, 2242
RseI CAYNNNNRTG 4 cut(s) 185, 473, 2097, 2195
SatI GCNGC 8 cut(s) 168, 237, 321, 359, 860, 1548, 2043, 2320
Sau96I GGNCC 1 cut(s) 920
ScaI AGTACT 1 cut(s) 820
SchI GAGTC 5 cut(s) 143, 550, 1685, 1961, 2016
ScrFI CCNGG 5 cut(s) 809, 932, 1258, 2223, 2449
SduI GDGCHC 1 cut(s) 505
SfaNI GCATC 9 cut(s) 154, 406, 428, 625, 697, 1087, 1797, 1981, 2029
SfcI CTRYAG 3 cut(s) 230, 1712, 2208
Sfr274I CTCGAG 1 cut(s) 1243
SinI GGWCC 1 cut(s) 920
SlaI CTCGAG 1 cut(s) 1243
SmiMI CAYNNNNRTG 4 cut(s) 185, 473, 2097, 2195
SmlI CTYRAG 3 cut(s) 1243, 1791, 2531
SmoI CTYRAG 3 cut(s) 1243, 1791, 2531
SseBI AGGCCT 2 cut(s) 532, 1476
SsiI CCGC 1 cut(s) 2320
SspI AATATT 1 cut(s) 2116
SspMI CTAG 9 cut(s) 59, 123, 387, 770, 777, 1979, 2123, 2408, 2465
StuI AGGCCT 2 cut(s) 532, 1476
StyD4I CCNGG 5 cut(s) 807, 930, 1256, 2221, 2447
StyI CCWWGG 3 cut(s) 1044, 1230, 1771
TaiI ACGT 5 cut(s) 461, 899, 1710, 2425, 2571
TaqI TCGA 7 cut(s) 22, 586, 1244, 1433, 1674, 1782, 2560
TatI WGTACW 2 cut(s) 818, 1163
TauI GCSGC 1 cut(s) 2322
TfiI GAWTC 5 cut(s) 398, 1667, 1755, 2585, 2594
TscAI CASTG 4 cut(s) 187, 408, 958, 2320
TseFI GTSAC 3 cut(s) 716, 2578, 2617
TseI GCWGC 7 cut(s) 167, 236, 320, 358, 859, 1547, 2042
Tsp45I GTSAC 3 cut(s) 716, 2578, 2617
TspGWI ACGGA 2 cut(s) 1025, 2639
TspRI CASTG 4 cut(s) 187, 408, 958, 2320
Van91I CCANNNNNTGG 1 cut(s) 2183
Vha464I CTTAAG 1 cut(s) 2531
VpaK11BI GGWCC 1 cut(s) 920
XapI RAATTY 9 cut(s) 118, 851, 1015, 1343, 1489, 1943, 1960, 2131, 2169
XbaI TCTAGA 2 cut(s) 769, 2407
XceI RCATGY 1 cut(s) 1183
XcmI CCANNNNNNNNNTGG 2 cut(s) 1960, 2383
XhoI CTCGAG 1 cut(s) 1243
XmiI GTMKAC 3 cut(s) 1053, 2020, 2201
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 9 cut(s) 59, 123, 387, 770, 777, 1979, 2123, 2408, 2465
ZraI GACGTC 1 cut(s) 1708
ZrmI AGTACT 1 cut(s) 820
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.