Rw2G034510

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000083
Physical Location & Seq
Forward (+)
56802786 .. 56816259
13474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G034510.1

Sequence Viewer

Length: 2652 bp
ATGTTTTCCATAGTTAGACTAAATCAACTTTTTCTTAGTTCTGATACTGAGATTTCTGACCATTTCAGCTTCATTAGCATAGACTGTGGCCTAGCAGCTAATTCTAGCTATAATGAACCAAAAACAGGCGTATTTTACGTGTCAGACACAACTTTCCTGGACAACATCACCGGTGAAAGTAAGCTCTTATTGCCAGAACACAGAGATGACTACCTACGGCAGGTCTGGAATCTCAGGTACTTTCCCGAAGGTATCCGCAACTGTTACACCATAAACATAATTAGTAGCACCAAATATTTGATCAGAGCAACATTCTTTTACGGGGATTATGATGGCCAACACAAGGCACCGGTGTTCGATTTGCATCTTGGAGCAAACTACTGGGATACAGTGAAGTCAAACAACACAATCAAGGAGATCATACATGTCCCCTTGCAAAACTACATTCACATTTGTCTGGTCAACACAGGCTTTGGGACTCCCTATATATCAGCGATAGAACTAAGGCCCTTGGGTACTACAGTTTACAAGACTCAAATGGGCTCCTTGGCACTATTCAATCGTTTGGACACTGGTTCAATAACGAATAAAACATACATGTATAACGACGACGTTCATGATCGCTGGTGGTACCCTTATTCTTACAATGGATGGACACAACTAAGTACCTCGCTTACCATTGACTCTGCAACAAGTGAAAATCCCTACCAACTGCCATCTATTGTCATGAGCACTGCTGCAACACCGAAAAATGAAAATGCTTCCTTGTATATTGGGTGGACTCCCCCGGACAGCAATACCGAATACTATGTCTACATGCACTTCGCTGAAGTTGAAAGTCTCCCAGCCAACCAATCTAGACTCTTGAGCATTACTTGGAATGGAAAGCCCTTCTATCGTCCTTTCAGTCCAGCATACTTATCCTCATTTACTGTATTCACCCAAGGGCCCTTGACTGGAGGGCTTCATAATTTTTCAATCTCTCAAGTAGGAAACTCTACCCTTCCGCCCATCATCAATGCCTTCGAGATTTATACAGTTATCGAGTTCTTACAGGAAGAAACAAACCAAGAAGAAGTGGACACCATGAAGAAAATCAAGTCAACCTATGGAGTGAAGAAAAATTGGCAAGGCGATCCATGTGCTCCTCAACAGTATGTGTGGGCAGGTTTAAACTGCAGTTACGATGGTTATGACTCCCCCAGAATCAATTCCTTAAACTTGTCCTTTAGTGGCTTAACTGGAGATATAGATCCATCTTTATCCAGTCTCACAATGCTACAGACTCTGGATTTATCTAACAACAATTTGACAGGACTGGTGCCTGACTTTTTGTCTCGATTGCCAAACTTAAAAGTCCTAAACTTGGAAAATAACAAGCTCACAGGTTCAGTTCCAGTTGGCTTAATTCAGAAAGCAAATGGTGGTTTGCTGTTATTGAGCTTGTGTGAAAACCAAAATCTAACTGCACCAGTTTCCTGCGGATCAAAGGAAAAGAAGAAGAACAAAAACAATTTTGTTGTTCCAGTAGTAGCGTCAGTTGTTGGAACTTTAGTCCTTTTATTATTAACTGCAGCAGCTGTTTGTTGGGGACTGACAAGGAGAAGGAAACAAGATGTTAATGATTCAATACCAACCATTCAGCCAGAGTCAATCGAGCCATCATCACGACGTCAATTTACTTACTCTGAGGTTCTCCAAATCACCAACAATTTTACGAGAATTATTGGTAGAGGAGGATTTGGAATAGTATATCATGGCACCATAGGTGACACTCAAGTAGCTGTGAAGTTGCTTTCACCATCTTCCGTTCAAGGATATCAAGAATTTCATTCAGAGGTTAATCTTCTAATGAGAGTTCATCACAAAAACTTGACGAGCCTTGTTGGATATTGCAAGGATGGAACCAATGCAGGGCTTATCTATGAATATATGGCTAATGGAAACTTACACAAACATCTTTCAGATCATAGCAGTTCATATATATTGAGTTGGGAGGAAAGACTTCGAATCGCAATTGATGCAGCACATGGACTGGAGTATCTGCACTATGGTTCCAGACCTCCAATAATCCACAGAGATGTGAAATCTACAAATATCTTGTTGAATGAAAACTTTCAAGCCAAACTATCTGATTTTGGCCTGTCTAGAATTTTTCCTGCTGAGGATGGGACTCGTCAATGGACACGTGTCGCTGGAACTCCTGGATACCTTGACCCTGAGTACTACCAATCAAACATGTTAAATGAAAAAAGTGATGTTTATAGCTTTGGGATTGTGCTGTTGGAGATAATCACAAGTAGACCTGTGATATCAAAGACACATGAGAAAATTCACATTGCTCAATGGGTTAATTTCATGGTTGAGAAAGGGGACATTACAAGTATCATAGATTCAAGGCTAAAAGGAAACTTTGATAGTAATTCTGTGTGGAAGGCTGTTGAAATAGCTATATTTTGTGTATCTTCAAGTTCCAACAACAGGCCAACCATGAGTCAGGTAGTGACAGAGCTGAAGGACTGCTTGGCTGCAGAATTGGCTCGAAAGGAGAGCTATGCAACAGAATCGAAAGATTCAGTTGAGATGATGCCACTGAAATTCACCGCTGAGATCAGACCCCTGCCTAGTACTATTGAAAAAAATCTCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

883

Amino Acids

99.14

Weight (kDa)

5.93

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 27 - 347 2.7e-111 Malectin-like domain
LRR_8 PF13855 401 - 461 1.2e-08 Leucine rich repeat
LRR_4 PF12799 426 - 462 5e-07 Leucine Rich repeats (2 copies)
PK_Tyr_Ser-Thr PF07714 572 - 838 5.9e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 573 - 836 1.9e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000083)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07560 AT1G51790 AT1G51790 AT1G51790 AT1G51800 AT1G51805 AT1G51805 AT1G51810 AT1G51820 AT1G51820 AT1G51830 AT1G51830 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51850 AT1G51850 AT1G51860 AT1G51860 AT1G51870 AT1G51870 AT1G51880 AT1G51880 AT1G51880 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51910 AT2G04300 AT2G04300 AT2G28960 AT2G28960 AT2G28970 AT2G28970 AT2G28990 AT2G28990 AT2G29000 AT3G21340 AT3G46350 AT5G59616 AT5G59650 AT5G59650 AT5G59660 AT5G59660 AT5G59660 AT5G59670 AT5G59670 AT5G59680
fragaria_vesca FvH4_2g03270 FvH4_3g21300 FvH4_6g32660 FvH4_6g32660 FvH4_6g32670 FvH4_6g32670 FvH4_6g32690 FvH4_6g32690 FvH4_6g32691 FvH4_6g32700 FvH4_6g32740 FvH4_6g32750 FvH4_6g33210 FvH4_6g33210 FvH4_7g24270 FvH4_7g24280 FvH4_7g24291 FvH4_7g24300
malus_domestica MD09G1198400.v1.1 MD09G1198600.v1.1 MD09G1199100.v1.1 MD09G1199200.v1.1 MD09G1199300.v1.1 MD09G1199500.v1.1 MD09G1199600.v1.1 MD17G1179300.v1.1 MD17G1180200.v1.1 MD17G1266000.v1.1
prunus_persica Prupe.1G438900_v2.0.a1 Prupe.1G439000_v2.0.a1 Prupe.1G439100_v2.0.a1 Prupe.3G019400_v2.0.a1 Prupe.3G021900_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G055100_v2.0.a1 Prupe.3G055400_v2.0.a1 Prupe.3G055500_v2.0.a1 Prupe.3G055600_v2.0.a1 Prupe.3G055700_v2.0.a1 Prupe.3G055800_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056100_v2.0.a1 Prupe.3G209600_v2.0.a1 Prupe.3G209700_v2.0.a1 Prupe.6G291100_v2.0.a1 Prupe.7G208900_v2.0.a1 Prupe.8G038300_v2.0.a1
pyrus_communis pycom09g11570 pycom09g11580 pycom09g11610 pycom09g11630 pycom09g11640 pycom17g18840 pycom17g18910 pycom17g18930 pycom17g18940
rosa_chinensis RchiOBHm_Chr1g0317521 RchiOBHm_Chr1g0317541 RchiOBHm_Chr2g0123351 RchiOBHm_Chr2g0141181 RchiOBHm_Chr2g0141191 RchiOBHm_Chr2g0141211 RchiOBHm_Chr2g0141221 RchiOBHm_Chr2g0141261 RchiOBHm_Chr2g0141271 RchiOBHm_Chr2g0141311 RchiOBHm_Chr2g0141331 RchiOBHm_Chr2g0141371 RchiOBHm_Chr2g0141391 RchiOBHm_Chr2g0141401 RchiOBHm_Chr2g0141431 RchiOBHm_Chr2g0141481 RchiOBHm_Chr2g0141491 RchiOBHm_Chr2g0141591 RchiOBHm_Chr2g0141601 RchiOBHm_Chr2g0141611 RchiOBHm_Chr2g0141631 RchiOBHm_Chr2g0141641 RchiOBHm_Chr2g0141661 RchiOBHm_Chr2g0141681 RchiOBHm_Chr2g0141701 RchiOBHm_Chr2g0141781 RchiOBHm_Chr2g0141811 RchiOBHm_Chr2g0141821 RchiOBHm_Chr3g0479311 RchiOBHm_Chr3g0495751 RchiOBHm_Chr4g0404741 RchiOBHm_Chr4g0410341 RchiOBHm_Chr4g0418371 RchiOBHm_Chr4g0438651 RchiOBHm_Chr5g0036411 RchiOBHm_Chr5g0036491 RchiOBHm_Chr5g0042391 RchiOBHm_Chr6g0279871 RchiOBHm_Chr7g0192921 RchiOBHm_Chr7g0214901 RchiOBHm_Chr7g0234931
rosa_laevigata RLG00000003432 RLG00000008806 RLG00000013062 RLG00000019514 RLG00000019923 RLG00000019924 RLG00000019925 RLG00000019930 RLG00000019931 RLG00000019934 RLG00000019937 RLG00000019938 RLG00000033697
rosa_multiflora Rmu_co8002302.1_g000001 Rmu_sc0000147.1_g000031 Rmu_sc0000463.1_g000005 Rmu_sc0000712.1_g000003 Rmu_sc0001483.1_g000017 Rmu_sc0001554.1_g000002 Rmu_sc0001554.1_g000005 Rmu_sc0001554.1_g000008 Rmu_sc0001981.1_g000017 Rmu_sc0002040.1_g000056 Rmu_sc0002755.1_g000018 Rmu_sc0002804.1_g000016 Rmu_sc0002928.1_g000015 Rmu_sc0003040.1_g000008 Rmu_sc0003040.1_g000018 Rmu_sc0004512.1_g000001 Rmu_sc0004512.1_g000004 Rmu_sc0005507.1_g000001 Rmu_sc0005507.1_g000009 Rmu_sc0005507.1_g000033 Rmu_sc0005507.1_g000037 Rmu_sc0005507.1_g000043 Rmu_sc0005507.1_g000047 Rmu_sc0006318.1_g000004 Rmu_sc0006318.1_g000014 Rmu_sc0006318.1_g000021 Rmu_sc0006556.1_g000001 Rmu_sc0007208.1_g000002 Rmu_sc0007208.1_g000003 Rmu_sc0007208.1_g000006 Rmu_sc0007208.1_g000008
rosa_roxburghii Rroxscaffold_1G00044430 Rroxscaffold_2G00103420 Rroxscaffold_2G00103470 Rroxscaffold_2G00103490 Rroxscaffold_2G00103500 Rroxscaffold_2G00103520 Rroxscaffold_2G00103530 Rroxscaffold_2G00103540 Rroxscaffold_2G00103550 Rroxscaffold_2G00103570 Rroxscaffold_2G00103770 Rroxscaffold_2G00103780 Rroxscaffold_2G00103800 Rroxscaffold_2G00103820 Rroxscaffold_2G00103860 Rroxscaffold_2G00103890 Rroxscaffold_2G00103930 Rroxscaffold_2G00103940 Rroxscaffold_2G00104010 Rroxscaffold_2G00104030 Rroxscaffold_2G00104050 Rroxscaffold_2G00104060 Rroxscaffold_2G00104070 Rroxscaffold_2G00104090 Rroxscaffold_2G00104100 Rroxscaffold_2G00104120 Rroxscaffold_2G00104130 Rroxscaffold_4G00331710
rosa_rugosa Rorug02G0367500 Rorug02G0367500 Rorug02G0367600 Rorug02G0367800 Rorug02G0367800 Rorug02G0367900 Rorug02G0368100 Rorug02G0368100 Rorug02G0368100 Rorug02G0368200 Rorug02G0368700 Rorug02G0368900 Rorug05G0155600 Rorug05G0155700 Rorug05G0155800 Rorug05G0156700 Rorug05G0157300
rosa_samantha Rh1AG028000 Rh2AG418700 Rh2AG418900 Rh2AG419000 Rh2AG419100 Rh2AG419400 Rh2AG419600 Rh2AG419800 Rh2AG420400 Rh2AG421000 Rh2BG428500 Rh2BG428600 Rh2BG428800 Rh2BG428900 Rh2BG429300 Rh2BG429400 Rh2BG429700 Rh2BG429900 Rh2BG430000 Rh2BG430700 Rh2CG405100 Rh2CG405200 Rh2CG405300 Rh2CG405500 Rh2CG405600 Rh2CG405700 Rh2CG406000 Rh2CG406100 Rh2CG406300 Rh2CG406600 Rh2CG406700 Rh2CG407500 Rh2CG408100 Rh2DG438500 Rh3DG123900 Rh3DG124000 Rh5BG249700 Rh5BG482500 Rh5CG281500 Rh5CG507300 Rh7DG133300
rosa_wichuraiana Rw0G019720 Rw0G019730 Rw0G019750 Rw0G023010 Rw2G034340 Rw2G034350 Rw2G034370 Rw2G034380 Rw2G034400 Rw2G034430 Rw2G034470 Rw2G034480 Rw2G034510 Rw5G022890 Rw5G026410 Rw5G032710 Rw5G042850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1675
Acc36I ACCTGC 2 cut(s) 211, 1157
Acc65I GGTACC 1 cut(s) 630
AccB1I GGYRCC 4 cut(s) 346, 630, 1321, 1760
AccI GTMKAC 2 cut(s) 813, 2302
AciI CCGC 4 cut(s) 256, 1007, 1482, 2604
AclWI GGATC 3 cut(s) 1130, 1247, 1492
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 4 cut(s) 1826, 2151, 2331, 2597
AcuI CTGAAG 2 cut(s) 849, 2534
AcvI CACGTG 1 cut(s) 2189
AcyI GRCGYC 1 cut(s) 1672
AfaI GTAC 6 cut(s) 239, 517, 632, 667, 2225, 2629
AfiI CCNNNNNNNGG 7 cut(s) 125, 220, 343, 956, 1366, 1914, 2481
AflIII ACRYGT 6 cut(s) 138, 424, 597, 2186, 2188, 2238
AgeI ACCGGT 2 cut(s) 170, 349
AhdI GACNNNNNGTC 1 cut(s) 1333
AjnI CCWGG 2 cut(s) 156, 2203
AjuI GAANNNNNNNTTGG 2 cut(s) 2507, 2539
Alw21I GWGCWC 2 cut(s) 734, 1147
Alw26I GTCTC 3 cut(s) 845, 1274, 1341
AlwI GGATC 3 cut(s) 1130, 1247, 1492
AlwNI CAGNNNCTG 2 cut(s) 1288, 1580
AoxI GGCC 6 cut(s) 88, 334, 506, 947, 2140, 2483
ApaI GGGCCC 1 cut(s) 951
ApeKI GCWGC 6 cut(s) 95, 737, 1574, 1577, 2024, 2528
ApoI RAATTY 4 cut(s) 1826, 2151, 2331, 2597
ArsI GACNNNNNNTTYG 2 cut(s) 795, 827
AsiGI ACCGGT 2 cut(s) 170, 349
Asp700I GAANNNNTTC 3 cut(s) 1210, 2004, 2115
Asp718I GGTACC 1 cut(s) 630
AspS9I GGNCC 3 cut(s) 507, 947, 948
AsuC2I CCSGG 1 cut(s) 788
AsuHPI GGTGA 7 cut(s) 160, 185, 931, 1696, 1781, 1791, 2593
AsuII TTCGAA 1 cut(s) 2008
BaeGI GKGCMC 1 cut(s) 951
BaeI ACNNNNGTAYC 4 cut(s) 507, 540, 2024, 2057
BalI TGGCCA 1 cut(s) 336
BanI GGYRCC 4 cut(s) 346, 630, 1321, 1760
BanII GRGCYC 2 cut(s) 545, 951
BbrPI CACGTG 1 cut(s) 2189
Bbv12I GWGCWC 2 cut(s) 734, 1147
BbvCI CCTCAGC 1 cut(s) 2163
BbvI GCAGC 6 cut(s) 107, 724, 1586, 1589, 2036, 2515
BceAI ACGGC 1 cut(s) 233
BcgI CGANNNNNNTGC 4 cut(s) 1124, 1158, 2547, 2581
BciT130I CCWGG 2 cut(s) 158, 2205
BciVI GTATCC 3 cut(s) 263, 379, 2201
BclI TGATCA 1 cut(s) 300
BcnI CCSGG 1 cut(s) 788
BcoDI GTCTC 3 cut(s) 845, 1274, 1341
BfaI CTAG 5 cut(s) 92, 105, 858, 2148, 2625
BfmI CTRYAG 5 cut(s) 519, 1177, 1280, 1572, 2529
BfuAI ACCTGC 2 cut(s) 211, 1157
BfuI GTATCC 3 cut(s) 263, 379, 2201
BisI GCNGC 6 cut(s) 96, 738, 1575, 1578, 2025, 2529
BlsI GCNGC 6 cut(s) 97, 739, 1576, 1579, 2026, 2530
BmcAI AGTACT 2 cut(s) 2225, 2629
Bme1390I CCNGG 3 cut(s) 158, 788, 2205
BmeRI GACNNNNNGTC 1 cut(s) 1333
BmgT120I GGNCC 3 cut(s) 507, 947, 948
BmiI GGNNCC 8 cut(s) 348, 544, 632, 949, 1323, 1762, 1906, 2056
BmrFI CCNGG 3 cut(s) 158, 788, 2205
BmrI ACTGGG 1 cut(s) 391
BmsI GCATC 3 cut(s) 373, 2011, 2577
BmuI ACTGGG 1 cut(s) 391
BoxI GACNNNNGTC 1 cut(s) 2189
BpmI CTGGAG 3 cut(s) 978, 1263, 2057
Bpu10I CCTNAGC 1 cut(s) 2163
Bpu14I TTCGAA 1 cut(s) 2008
BpuEI CTTGAG 3 cut(s) 886, 969, 1761
BpuMI CCSGG 1 cut(s) 788
BsaAI YACGTR 2 cut(s) 139, 2189
BsaBI GATNNNNATC 2 cut(s) 363, 1251
BsaHI GRCGYC 1 cut(s) 1672
BsaJI CCNNGG 4 cut(s) 510, 546, 786, 943
BsaWI WCCGGW 2 cut(s) 170, 349
Bsc4I CCNNNNNNNGG 7 cut(s) 125, 220, 343, 956, 1366, 1914, 2481
Bse118I RCCGGY 2 cut(s) 170, 349
Bse3DI GCAATG 1 cut(s) 2337
Bse8I GATNNNNATC 2 cut(s) 363, 1251
BseBI CCWGG 2 cut(s) 158, 2205
BseDI CCNNGG 4 cut(s) 510, 546, 786, 943
BseGI GGATG 3 cut(s) 656, 1906, 2173
BseJI GATNNNNATC 2 cut(s) 363, 1251
BseLI CCNNNNNNNGG 7 cut(s) 125, 220, 343, 956, 1366, 1914, 2481
BseMI GCAATG 1 cut(s) 2337
BseMII CTCAG 6 cut(s) 39, 247, 1680, 2154, 2211, 2598
BseRI GAGGAG 2 cut(s) 1137, 1749
BseSI GKGCMC 1 cut(s) 951
BseXI GCAGC 6 cut(s) 107, 724, 1586, 1589, 2036, 2515
BseYI CCCAGC 1 cut(s) 844
BsgI GTGCAG 2 cut(s) 1452, 2030
BshFI GGCC 6 cut(s) 90, 336, 508, 949, 2142, 2485
BshNI GGYRCC 4 cut(s) 346, 630, 1321, 1760
BshTI ACCGGT 2 cut(s) 170, 349
BsiHKAI GWGCWC 2 cut(s) 734, 1147
BsiSI CCGG 3 cut(s) 171, 350, 788
BslFI GGGAC 5 cut(s) 413, 490, 1605, 2185, 2387
BslI CCNNNNNNNGG 7 cut(s) 125, 220, 343, 956, 1366, 1914, 2481
BsmAI GTCTC 3 cut(s) 845, 1274, 1341
BsmFI GGGAC 5 cut(s) 413, 490, 1605, 2185, 2387
BsnI GGCC 6 cut(s) 90, 336, 508, 949, 2142, 2485
Bsp119I TTCGAA 1 cut(s) 2008
Bsp120I GGGCCC 1 cut(s) 947
Bsp1286I GDGCHC 4 cut(s) 545, 734, 951, 1147
Bsp143I GATC 8 cut(s) 300, 417, 619, 1135, 1252, 1484, 1966, 2610
BspACI CCGC 4 cut(s) 256, 1007, 1482, 2604
BspANI GGCC 6 cut(s) 90, 336, 508, 949, 2142, 2485
BspCNI CTCAG 6 cut(s) 40, 246, 1681, 2155, 2212, 2599
BspHI TCATGA 2 cut(s) 616, 726
BspLI GGNNCC 8 cut(s) 348, 544, 632, 949, 1323, 1762, 1906, 2056
BspMAI CTGCAG 3 cut(s) 1181, 1576, 2533
BspMI ACCTGC 2 cut(s) 211, 1157
BspPI GGATC 3 cut(s) 1130, 1247, 1492
BspT104I TTCGAA 1 cut(s) 2008
BspT107I GGYRCC 4 cut(s) 346, 630, 1321, 1760
BsrDI GCAATG 1 cut(s) 2337
BsrFI RCCGGY 2 cut(s) 170, 349
BssAI RCCGGY 2 cut(s) 170, 349
BssECI CCNNGG 4 cut(s) 510, 546, 786, 943
BssMI GATC 8 cut(s) 300, 417, 619, 1135, 1252, 1484, 1966, 2610
BssNI GRCGYC 1 cut(s) 1672
BssT1I CCWWGG 3 cut(s) 510, 546, 943
Bst2UI CCWGG 2 cut(s) 158, 2205
Bst4CI ACNGT 7 cut(s) 86, 263, 391, 523, 934, 1039, 1155
BstACI GRCGYC 1 cut(s) 1672
BstAPI GCANNNNNTGC 1 cut(s) 2021
BstBAI YACGTR 2 cut(s) 139, 2189
BstBI TTCGAA 1 cut(s) 2008
BstDEI CTNAG 9 cut(s) 35, 48, 233, 503, 662, 1689, 2163, 2220, 2607
BstENI CCTNNNNNAGG 1 cut(s) 218
BstF5I GGATG 3 cut(s) 656, 1906, 2173
BstKTI GATC 8 cut(s) 303, 420, 622, 1138, 1255, 1487, 1969, 2613
BstMAI GTCTC 3 cut(s) 845, 1274, 1341
BstMBI GATC 8 cut(s) 300, 417, 619, 1135, 1252, 1484, 1966, 2610
BstMWI GCNNNNNNNGC 4 cut(s) 75, 190, 2021, 2537
BstNI CCWGG 2 cut(s) 158, 2205
BstNSI RCATGY 4 cut(s) 428, 601, 820, 2242
BstPAI GACNNNNGTC 1 cut(s) 2189
BstSCI CCNGG 3 cut(s) 156, 786, 2203
BstSFI CTRYAG 5 cut(s) 519, 1177, 1280, 1572, 2529
BstSLI GKGCMC 1 cut(s) 951
BstV1I GCAGC 6 cut(s) 107, 724, 1586, 1589, 2036, 2515
BstX2I RGATCY 1 cut(s) 1252
BstYI RGATCY 1 cut(s) 1252
BsuI GTATCC 3 cut(s) 263, 379, 2201
BsuRI GGCC 6 cut(s) 90, 336, 508, 949, 2142, 2485
BtsCI GGATG 3 cut(s) 656, 1906, 2173
BtsI GCAGTG 1 cut(s) 732
BtsIMutI CAGTG 4 cut(s) 396, 570, 732, 2591
BveI ACCTGC 2 cut(s) 211, 1157
CaiI CAGNNNCTG 2 cut(s) 1288, 1580
CciI TCATGA 2 cut(s) 616, 726
Cfr10I RCCGGY 2 cut(s) 170, 349
Cfr13I GGNCC 3 cut(s) 507, 947, 948
CseI GACGC 1 cut(s) 1524
Csp6I GTAC 6 cut(s) 238, 516, 631, 666, 2224, 2628
CspAI ACCGGT 2 cut(s) 170, 349
CviQI GTAC 6 cut(s) 238, 516, 631, 666, 2224, 2628
DdeI CTNAG 9 cut(s) 35, 48, 233, 503, 662, 1689, 2163, 2220, 2607
DpnI GATC 8 cut(s) 302, 419, 621, 1137, 1254, 1486, 1968, 2612
DpnII GATC 8 cut(s) 300, 417, 619, 1135, 1252, 1484, 1966, 2610
DraI TTTAAA 1 cut(s) 1173
DriI GACNNNNNGTC 1 cut(s) 1333
EaeI YGGCCR 1 cut(s) 334
Eam1105I GACNNNNNGTC 1 cut(s) 1333
EciI GGCGGA 1 cut(s) 996
Eco130I CCWWGG 3 cut(s) 510, 546, 943
Eco24I GRGCYC 2 cut(s) 545, 951
Eco32I GATATC 2 cut(s) 1820, 2313
Eco57I CTGAAG 2 cut(s) 849, 2534
Eco72I CACGTG 1 cut(s) 2189
EcoNI CCTNNNNNAGG 1 cut(s) 218
EcoO109I RGGNCCY 3 cut(s) 507, 947, 948
EcoRII CCWGG 2 cut(s) 156, 2203
EcoRV GATATC 2 cut(s) 1820, 2313
EcoT14I CCWWGG 3 cut(s) 510, 546, 943
EcoT38I GRGCYC 2 cut(s) 545, 951
ErhI CCWWGG 3 cut(s) 510, 546, 943
FalI AAGNNNNNCTT 2 cut(s) 2507, 2539
FaqI GGGAC 5 cut(s) 413, 490, 1605, 2185, 2387
FbaI TGATCA 1 cut(s) 300
FblI GTMKAC 2 cut(s) 813, 2302
Fnu4HI GCNGC 6 cut(s) 96, 738, 1575, 1578, 2025, 2529
FokI GGATG 3 cut(s) 663, 1913, 2180
FriOI GRGCYC 2 cut(s) 545, 951
Fsp4HI GCNGC 6 cut(s) 96, 738, 1575, 1578, 2025, 2529
FspBI CTAG 5 cut(s) 92, 105, 858, 2148, 2625
GluI GCNGC 6 cut(s) 96, 738, 1575, 1578, 2025, 2529
GsaI CCCAGC 1 cut(s) 848
GsuI CTGGAG 3 cut(s) 978, 1263, 2057
HaeIII GGCC 6 cut(s) 90, 336, 508, 949, 2142, 2485
HapII CCGG 3 cut(s) 171, 350, 788
HgaI GACGC 1 cut(s) 1524
Hin1I GRCGYC 1 cut(s) 1672
HincII GTYRAC 2 cut(s) 463, 1104
HindII GTYRAC 2 cut(s) 463, 1104
HpaII CCGG 3 cut(s) 171, 350, 788
HphI GGTGA 7 cut(s) 160, 185, 931, 1696, 1781, 1791, 2593
Hpy166II GTNNAC 7 cut(s) 463, 526, 780, 814, 1081, 1104, 2303
Hpy8I GTNNAC 7 cut(s) 463, 526, 780, 814, 1081, 1104, 2303
Hpy99I CGWCG 3 cut(s) 611, 614, 1674
HpyAV CCTTC 7 cut(s) 242, 901, 1013, 1033, 1599, 2428, 2509
HpyCH4III ACNGT 7 cut(s) 86, 263, 391, 523, 934, 1039, 1155
HpyCH4IV ACGT 4 cut(s) 138, 612, 1672, 2188
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 190, 2021, 2537
HpyF3I CTNAG 9 cut(s) 35, 48, 233, 503, 662, 1689, 2163, 2220, 2607
HpySE526I ACGT 4 cut(s) 138, 612, 1672, 2188
Hsp92I GRCGYC 1 cut(s) 1672
KpnI GGTACC 1 cut(s) 634
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 8 cut(s) 300, 417, 619, 1135, 1252, 1484, 1966, 2610
LmnI GCTCC 3 cut(s) 371, 548, 1150
Lsp1109I GCAGC 6 cut(s) 107, 724, 1586, 1589, 2036, 2515
LweI GCATC 3 cut(s) 373, 2011, 2577
MaeI CTAG 5 cut(s) 92, 105, 858, 2148, 2625
MaeII ACGT 4 cut(s) 138, 612, 1672, 2188
MaeIII GTNAC 4 cut(s) 263, 1181, 1769, 2503
MalI GATC 8 cut(s) 302, 419, 621, 1137, 1254, 1486, 1968, 2612
MboI GATC 8 cut(s) 300, 417, 619, 1135, 1252, 1484, 1966, 2610
MboII GAAGA 9 cut(s) 1070, 1085, 1102, 1129, 1510, 1513, 1797, 1838, 2457
MfeI CAATTG 1 cut(s) 2016
MflI RGATCY 1 cut(s) 1252
MhlI GDGCHC 4 cut(s) 545, 734, 951, 1147
MlsI TGGCCA 1 cut(s) 336
MluNI TGGCCA 1 cut(s) 336
MmeI TCCRAC 4 cut(s) 1525, 1867, 2265, 2499
Mox20I TGGCCA 1 cut(s) 336
MroXI GAANNNNTTC 3 cut(s) 1210, 2004, 2115
MscI TGGCCA 1 cut(s) 336
MslI CAYNNNNRTG 2 cut(s) 204, 2079
Msp20I TGGCCA 1 cut(s) 336
MspA1I CMGCKG 2 cut(s) 1580, 2606
MspI CCGG 3 cut(s) 171, 350, 788
MspR9I CCNGG 3 cut(s) 158, 788, 2205
MssI GTTTAAAC 1 cut(s) 1173
MunI CAATTG 1 cut(s) 2016
MvaI CCWGG 2 cut(s) 158, 2205
MwoI GCNNNNNNNGC 4 cut(s) 75, 190, 2021, 2537
NciI CCSGG 1 cut(s) 788
NdeII GATC 8 cut(s) 300, 417, 619, 1135, 1252, 1484, 1966, 2610
NlaIV GGNNCC 8 cut(s) 348, 544, 632, 949, 1323, 1762, 1906, 2056
NmuCI GTSAC 2 cut(s) 1769, 2503
NspI RCATGY 4 cut(s) 428, 601, 820, 2242
NspV TTCGAA 1 cut(s) 2008
PagI TCATGA 2 cut(s) 616, 726
PciI ACATGT 3 cut(s) 424, 597, 2238
PdmI GAANNNNTTC 3 cut(s) 1210, 2004, 2115
PfeI GAWTC 7 cut(s) 229, 1206, 1625, 2010, 2393, 2564, 2573
PfoI TCCNGGA 2 cut(s) 156, 2203
PinAI ACCGGT 2 cut(s) 170, 349
PkrI GCNGC 6 cut(s) 97, 739, 1576, 1579, 2026, 2530
PmaCI CACGTG 1 cut(s) 2189
PmeI GTTTAAAC 1 cut(s) 1173
PmlI CACGTG 1 cut(s) 2189
Ppu21I YACGTR 2 cut(s) 139, 2189
PscI ACATGT 3 cut(s) 424, 597, 2238
PshAI GACNNNNGTC 1 cut(s) 2189
Psp6I CCWGG 2 cut(s) 156, 2203
PspCI CACGTG 1 cut(s) 2189
PspFI CCCAGC 1 cut(s) 844
PspGI CCWGG 2 cut(s) 156, 2203
PspN4I GGNNCC 8 cut(s) 348, 544, 632, 949, 1323, 1762, 1906, 2056
PspOMI GGGCCC 1 cut(s) 947
PspPI GGNCC 3 cut(s) 507, 947, 948
PstI CTGCAG 3 cut(s) 1181, 1576, 2533
PstNI CAGNNNCTG 2 cut(s) 1288, 1580
PsuI RGATCY 1 cut(s) 1252
PvuII CAGCTG 1 cut(s) 1580
RsaI GTAC 6 cut(s) 239, 517, 632, 667, 2225, 2629
RsaNI GTAC 6 cut(s) 238, 516, 631, 666, 2224, 2628
RseI CAYNNNNRTG 2 cut(s) 204, 2079
SatI GCNGC 6 cut(s) 96, 738, 1575, 1578, 2025, 2529
Sau3AI GATC 8 cut(s) 300, 417, 619, 1135, 1252, 1484, 1966, 2610
Sau96I GGNCC 3 cut(s) 507, 947, 948
ScaI AGTACT 2 cut(s) 2225, 2629
ScrFI CCNGG 3 cut(s) 158, 788, 2205
SduI GDGCHC 4 cut(s) 545, 734, 951, 1147
SfaNI GCATC 3 cut(s) 373, 2011, 2577
SfcI CTRYAG 5 cut(s) 519, 1177, 1280, 1572, 2529
SfuI TTCGAA 1 cut(s) 2008
SgrAI CRCCGGYG 2 cut(s) 170, 349
SmiMI CAYNNNNRTG 2 cut(s) 204, 2079
SmlI CTYRAG 3 cut(s) 865, 984, 1776
SmoI CTYRAG 3 cut(s) 865, 984, 1776
SsiI CCGC 4 cut(s) 256, 1007, 1482, 2604
SspI AATATT 1 cut(s) 296
SspMI CTAG 5 cut(s) 92, 105, 858, 2148, 2625
StyD4I CCNGG 3 cut(s) 156, 786, 2203
StyI CCWWGG 3 cut(s) 510, 546, 943
TaaI ACNGT 7 cut(s) 86, 263, 391, 523, 934, 1039, 1155
TaiI ACGT 4 cut(s) 141, 615, 1675, 2191
TaqI TCGA 9 cut(s) 357, 1026, 1044, 1339, 1656, 2008, 2542, 2567, 2646
TatI WGTACW 2 cut(s) 2223, 2627
TfiI GAWTC 7 cut(s) 229, 1206, 1625, 2010, 2393, 2564, 2573
TscAI CASTG 4 cut(s) 396, 577, 739, 2598
TseFI GTSAC 2 cut(s) 1769, 2503
TseI GCWGC 6 cut(s) 95, 737, 1574, 1577, 2024, 2528
Tsp45I GTSAC 2 cut(s) 1769, 2503
TspGWI ACGGA 1 cut(s) 1798
TspRI CASTG 4 cut(s) 396, 577, 739, 2598
XagI CCTNNNNNAGG 1 cut(s) 218
XapI RAATTY 4 cut(s) 1826, 2151, 2331, 2597
XbaI TCTAGA 2 cut(s) 857, 2147
XceI RCATGY 4 cut(s) 428, 601, 820, 2242
XmiI GTMKAC 2 cut(s) 813, 2302
XmnI GAANNNNTTC 3 cut(s) 1210, 2004, 2115
XspI CTAG 5 cut(s) 92, 105, 858, 2148, 2625
ZraI GACGTC 1 cut(s) 1673
ZrmI AGTACT 2 cut(s) 2225, 2629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.