Prupe.3G055600_v2.0.a1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000083
Physical Location & Seq
Forward (+)
3937808 .. 3945754
7947 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G055600.1

Sequence Viewer

Length: 2490 bp
ATGTGTTTCTATATCTCAGGGTTCATTAGCATAGATTGCGGCCTCCAGACAGATTTAGGTTATTCTGAAAAGAGAACTGGCATTAACTACATTTATGACGCAACCTTCATAGACACCGGTGAAAGTAAATTCACATTGCCTAGTCACAGAGATGACTATCAACAGCCATACTGGTCTGTTAGGAGCTTCCCCGAAGGAGCCAGGAATTGCTACAAGATAAATGTTACACGCGGCAACAAATATTTGATCCGAGCAAGTTTTTTATATGGGAATTATGATGGCCAAGATAAGATACCAGAATTTGAACTGCACCTTGGACCTAATTTGTGGGATACTGTGAGCCTTAGAACCTCCGAACCCAATGACAAAGAGCTCATATATGTCCCACTCCGAAACTATATACATGTTTGTTTGGTGAACACAGGCTCCGGGGTTCCATTTATATCTGCTCTAGAACTCAGGCCTTTACCTAATGCATCTTATCAAACGAAAACGGGGTCATTGGCACTTGTCTCCCGGTATGACACGGGCCAAAGACATAATAGGACAGGATACAGGTATCCACTCGATATCCTTGATCGAATCTGGTGTGCCTATAATGACCTTGATAATTGGACACAATTAGACACCATATCCACTATCAACTCTGACTCTGGCGACCCTTACCAACTTCCATCTGTTGTTATGCGCACTGCTTCCACGCCAAAAAATCCATGTGATTCCTTGAGTATTATTTTCTCGTTGCCAGACAAAGATGCTGAATATTACGGTTACCTGCACTTTGCAGAAGTTGAAATCCTCAAACTCAACCAATCTAGATTGCAATACGTTTTTTGGGACGGAGCCGGCACTTTTGGGCCATTTGCTCCTCAATACTTGTCAACAAATACCATTTACAGCACTAACGCTTGGAGCACTCGTGGACCATATGCTAATATCTCAATCACCAGGGCTAAGAACTCTACCCTTCCACCTATCCTCAATGCTTTCGAGATTTATATGGTTAAACATTTCGTAGAAGCAGAAACAAACCAAGAAGATGTTGACGCAATCACATCGATCAAGTCAACTTATAATATTAAAAGAAATTGGCAAGGAGATCCATGTGCCCCTCAAGATTACATTTGGCAAGGAGTAAAGTGTAACTATCAAGAATTTGAGTCACCAAGAATCATATCCTTTGAAGTTACAAAACATACAATCAGGAACTTGTCCTCGAGTGGCTTAACAGGGGAGATAGCTGCTTCTATATCCAATCTCACAATGATACAGTCTTTGGATCTATCAAACAACAACTTAACGGGACCAATTCCAGAATTTTTGTCTAAATTCCTGAATTTGACTGTCCTAAACTTGGAGAAAAACAAGTTCACAGGCTCGGTTCCGGTAGGACTGATTGAAAGAAAGAATAGTGGTTTTCTATCACTAAGTTTGTGCGATAATGCACATCTATCTCGACATGTTTCTTGCATATTGAAGAAGAAGCACAGTTTCGTTATTCCCATAGTGTCAATCGCTGGAATTTTCATCCTCTTAGTTGTAGCAGCTATCTGCTGGTGGGGCTTCAAAAGGAAAAGGCAACATGAAAACATCATGGTTCTTCGTACAGAGTCAAAAACGTATTGTGAGCATCATAGCATATCCAGCCAAGTACCAACCGCCACCTTCAATTTTCTTGATCCAAAACCCCAGCTGACAAAATCAACAAAGCGACAGTTCACATATTCTGAGATACTAAAGATGACCAACAACTTTGAGAGGGTCCTTGGCAAAGGTGGATTTGGAACCGTGTATCATGGCTACATGCCGTGTGTGATGACACTTGGCCATGTGAGGAGAGAGGCAGAGAGGGGCGGCGAAAAAGAGACAGTGAGACCCCACACCAAGCCCAACCATTACGCTTCTACCCATGATACTTACGGTGGTCAAAACCCCAAGATAAAGAACAACTATTATCGATCCTTCAACAATGGAAAACGTAGCAGGCATTCCAACACTTATCGGTCTGGAGGCAACAACCCCTCAAAGACTAACAATCGTGCCCCGCTTCTCTTTGAGCCTAACCCAGAAGAGGAGAAGCGTGGTGTCATTTTCCGCCACAATGACTCGTTGATCATTCGAGTGGATATTTCCAACTTCGATGTTGGCCGTATTCTCGTGGACACTAGTAGCTCGGTGAGTGTCATGTTTGGAGAAGCCTTGAACGAACTTCAGGTCCCTGACCACCTGCTTGACCAAACTGTTTTCTGGATGGGCCTTAGATGGAAACGACAACATGAGATGATGAAGAATTTCAAACCAAATAGGTTGGTGGAGTGCGAGGGTCAACACTTTGCATACTCGGAGATTGTGAAGATTACTAACGATTTTGCATCAACAATTGGAAGAGGCGGATTTGGAAAAGTCTACCTTGGCACTCTTACACATGAGACCCAGGTTGCTGTGAAGTTGCTCAATTCATCGTCGAGGCAAGGCTCAAAAGAATTTTAA

Protein Analysis

830

Amino Acids

94.24

Weight (kDa)

8.78

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000083)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07560 AT1G51790 AT1G51790 AT1G51790 AT1G51800 AT1G51805 AT1G51805 AT1G51810 AT1G51820 AT1G51820 AT1G51830 AT1G51830 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51850 AT1G51850 AT1G51860 AT1G51860 AT1G51870 AT1G51870 AT1G51880 AT1G51880 AT1G51880 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51910 AT2G04300 AT2G04300 AT2G28960 AT2G28960 AT2G28970 AT2G28970 AT2G28990 AT2G28990 AT2G29000 AT3G21340 AT3G46350 AT5G59616 AT5G59650 AT5G59650 AT5G59660 AT5G59660 AT5G59660 AT5G59670 AT5G59670 AT5G59680
fragaria_vesca FvH4_2g03270 FvH4_3g21300 FvH4_6g32660 FvH4_6g32660 FvH4_6g32670 FvH4_6g32670 FvH4_6g32690 FvH4_6g32690 FvH4_6g32691 FvH4_6g32700 FvH4_6g32740 FvH4_6g32750 FvH4_6g33210 FvH4_6g33210 FvH4_7g24270 FvH4_7g24280 FvH4_7g24291 FvH4_7g24300
malus_domestica MD09G1198400.v1.1 MD09G1198600.v1.1 MD09G1199100.v1.1 MD09G1199200.v1.1 MD09G1199300.v1.1 MD09G1199500.v1.1 MD09G1199600.v1.1 MD17G1179300.v1.1 MD17G1180200.v1.1 MD17G1266000.v1.1
prunus_persica Prupe.1G438900_v2.0.a1 Prupe.1G439000_v2.0.a1 Prupe.1G439100_v2.0.a1 Prupe.3G019400_v2.0.a1 Prupe.3G021900_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G055100_v2.0.a1 Prupe.3G055400_v2.0.a1 Prupe.3G055500_v2.0.a1 Prupe.3G055600_v2.0.a1 Prupe.3G055700_v2.0.a1 Prupe.3G055800_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056100_v2.0.a1 Prupe.3G209600_v2.0.a1 Prupe.3G209700_v2.0.a1 Prupe.6G291100_v2.0.a1 Prupe.7G208900_v2.0.a1 Prupe.8G038300_v2.0.a1
pyrus_communis pycom09g11570 pycom09g11580 pycom09g11610 pycom09g11630 pycom09g11640 pycom17g18840 pycom17g18910 pycom17g18930 pycom17g18940
rosa_chinensis RchiOBHm_Chr1g0317521 RchiOBHm_Chr1g0317541 RchiOBHm_Chr2g0123351 RchiOBHm_Chr2g0141181 RchiOBHm_Chr2g0141191 RchiOBHm_Chr2g0141211 RchiOBHm_Chr2g0141221 RchiOBHm_Chr2g0141261 RchiOBHm_Chr2g0141271 RchiOBHm_Chr2g0141311 RchiOBHm_Chr2g0141331 RchiOBHm_Chr2g0141371 RchiOBHm_Chr2g0141391 RchiOBHm_Chr2g0141401 RchiOBHm_Chr2g0141431 RchiOBHm_Chr2g0141481 RchiOBHm_Chr2g0141491 RchiOBHm_Chr2g0141591 RchiOBHm_Chr2g0141601 RchiOBHm_Chr2g0141611 RchiOBHm_Chr2g0141631 RchiOBHm_Chr2g0141641 RchiOBHm_Chr2g0141661 RchiOBHm_Chr2g0141681 RchiOBHm_Chr2g0141701 RchiOBHm_Chr2g0141781 RchiOBHm_Chr2g0141811 RchiOBHm_Chr2g0141821 RchiOBHm_Chr3g0479311 RchiOBHm_Chr3g0495751 RchiOBHm_Chr4g0404741 RchiOBHm_Chr4g0410341 RchiOBHm_Chr4g0418371 RchiOBHm_Chr4g0438651 RchiOBHm_Chr5g0036411 RchiOBHm_Chr5g0036491 RchiOBHm_Chr5g0042391 RchiOBHm_Chr6g0279871 RchiOBHm_Chr7g0192921 RchiOBHm_Chr7g0214901 RchiOBHm_Chr7g0234931
rosa_laevigata RLG00000003432 RLG00000008806 RLG00000013062 RLG00000019514 RLG00000019923 RLG00000019924 RLG00000019925 RLG00000019930 RLG00000019931 RLG00000019934 RLG00000019937 RLG00000019938 RLG00000033697
rosa_multiflora Rmu_co8002302.1_g000001 Rmu_sc0000147.1_g000031 Rmu_sc0000463.1_g000005 Rmu_sc0000712.1_g000003 Rmu_sc0001483.1_g000017 Rmu_sc0001554.1_g000002 Rmu_sc0001554.1_g000005 Rmu_sc0001554.1_g000008 Rmu_sc0001981.1_g000017 Rmu_sc0002040.1_g000056 Rmu_sc0002755.1_g000018 Rmu_sc0002804.1_g000016 Rmu_sc0002928.1_g000015 Rmu_sc0003040.1_g000008 Rmu_sc0003040.1_g000018 Rmu_sc0004512.1_g000001 Rmu_sc0004512.1_g000004 Rmu_sc0005507.1_g000001 Rmu_sc0005507.1_g000009 Rmu_sc0005507.1_g000033 Rmu_sc0005507.1_g000037 Rmu_sc0005507.1_g000043 Rmu_sc0005507.1_g000047 Rmu_sc0006318.1_g000004 Rmu_sc0006318.1_g000014 Rmu_sc0006318.1_g000021 Rmu_sc0006556.1_g000001 Rmu_sc0007208.1_g000002 Rmu_sc0007208.1_g000003 Rmu_sc0007208.1_g000006 Rmu_sc0007208.1_g000008
rosa_roxburghii Rroxscaffold_1G00044430 Rroxscaffold_2G00103420 Rroxscaffold_2G00103470 Rroxscaffold_2G00103490 Rroxscaffold_2G00103500 Rroxscaffold_2G00103520 Rroxscaffold_2G00103530 Rroxscaffold_2G00103540 Rroxscaffold_2G00103550 Rroxscaffold_2G00103570 Rroxscaffold_2G00103770 Rroxscaffold_2G00103780 Rroxscaffold_2G00103800 Rroxscaffold_2G00103820 Rroxscaffold_2G00103860 Rroxscaffold_2G00103890 Rroxscaffold_2G00103930 Rroxscaffold_2G00103940 Rroxscaffold_2G00104010 Rroxscaffold_2G00104030 Rroxscaffold_2G00104050 Rroxscaffold_2G00104060 Rroxscaffold_2G00104070 Rroxscaffold_2G00104090 Rroxscaffold_2G00104100 Rroxscaffold_2G00104120 Rroxscaffold_2G00104130 Rroxscaffold_4G00331710
rosa_rugosa Rorug02G0367500 Rorug02G0367500 Rorug02G0367600 Rorug02G0367800 Rorug02G0367800 Rorug02G0367900 Rorug02G0368100 Rorug02G0368100 Rorug02G0368100 Rorug02G0368200 Rorug02G0368700 Rorug02G0368900 Rorug05G0155600 Rorug05G0155700 Rorug05G0155800 Rorug05G0156700 Rorug05G0157300
rosa_samantha Rh1AG028000 Rh2AG418700 Rh2AG418900 Rh2AG419000 Rh2AG419100 Rh2AG419400 Rh2AG419600 Rh2AG419800 Rh2AG420400 Rh2AG421000 Rh2BG428500 Rh2BG428600 Rh2BG428800 Rh2BG428900 Rh2BG429300 Rh2BG429400 Rh2BG429700 Rh2BG429900 Rh2BG430000 Rh2BG430700 Rh2CG405100 Rh2CG405200 Rh2CG405300 Rh2CG405500 Rh2CG405600 Rh2CG405700 Rh2CG406000 Rh2CG406100 Rh2CG406300 Rh2CG406600 Rh2CG406700 Rh2CG407500 Rh2CG408100 Rh2DG438500 Rh3DG123900 Rh3DG124000 Rh5BG249700 Rh5BG482500 Rh5CG281500 Rh5CG507300 Rh7DG133300
rosa_wichuraiana Rw0G019720 Rw0G019730 Rw0G019750 Rw0G023010 Rw2G034340 Rw2G034350 Rw2G034370 Rw2G034380 Rw2G034400 Rw2G034430 Rw2G034470 Rw2G034480 Rw2G034510 Rw5G022890 Rw5G026410 Rw5G032710 Rw5G042850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1074
AarI CACCTGC 1 cut(s) 2235
Acc16I TGCGCA 1 cut(s) 689
Acc36I ACCTGC 2 cut(s) 783, 2235
AccI GTMKAC 1 cut(s) 2406
AccII CGCG 1 cut(s) 231
AciI CCGC 7 cut(s) 39, 231, 1659, 1854, 2045, 2095, 2391
AclWI GGATC 5 cut(s) 241, 1094, 1287, 1673, 1953
AcoI YGGCCR 3 cut(s) 280, 1825, 2146
AcsI RAATTY 9 cut(s) 128, 299, 1154, 1316, 1328, 1336, 1521, 2290, 2483
AcuI CTGAAG 1 cut(s) 2195
AfaI GTAC 2 cut(s) 1606, 1653
AfiI CCNNNNNNNGG 2 cut(s) 1354, 2071
AflIII ACRYGT 2 cut(s) 403, 1459
AgeI ACCGGT 1 cut(s) 116
AhlI ACTAGT 1 cut(s) 2165
AjnI CCWGG 3 cut(s) 200, 947, 2433
AjuI GAANNNNNNNTTGG 2 cut(s) 297, 329
AloI GAACNNNNNNTCC 4 cut(s) 410, 442, 2199, 2231
AluBI AGCT 6 cut(s) 186, 373, 1241, 1547, 1693, 2172
AluI AGCT 6 cut(s) 186, 373, 1241, 1547, 1693, 2172
Alw21I GWGCWC 2 cut(s) 375, 917
Alw26I GTCTC 4 cut(s) 517, 1859, 1867, 2423
AlwI GGATC 5 cut(s) 241, 1094, 1287, 1673, 1953
Ama87I CYCGRG 1 cut(s) 1216
AoxI GGCC 8 cut(s) 40, 280, 461, 529, 857, 1825, 2146, 2254
ApeKI GCWGC 2 cut(s) 1241, 1544
ApoI RAATTY 9 cut(s) 128, 299, 1154, 1316, 1328, 1336, 1521, 2290, 2483
ArsI GACNNNNNNTTYG 4 cut(s) 482, 514, 2199, 2231
AsiGI ACCGGT 1 cut(s) 116
Asp700I GAANNNNTTC 1 cut(s) 2291
AspLEI GCGC 1 cut(s) 690
AspS9I GGNCC 8 cut(s) 317, 529, 857, 923, 1304, 1762, 2215, 2254
AsuC2I CCSGG 2 cut(s) 430, 517
AsuHPI GGTGA 5 cut(s) 131, 427, 937, 1155, 2188
AvaI CYCGRG 1 cut(s) 1216
AvaII GGWCC 5 cut(s) 317, 923, 1304, 1762, 2215
BaeGI GKGCMC 2 cut(s) 1111, 2044
BalI TGGCCA 2 cut(s) 282, 1827
BanII GRGCYC 1 cut(s) 375
BauI CACGAG 2 cut(s) 918, 2156
Bbv12I GWGCWC 2 cut(s) 375, 917
BbvI GCAGC 2 cut(s) 1228, 1556
BccI CCATC 4 cut(s) 272, 682, 2245, 2256
BceAI ACGGC 2 cut(s) 1792, 2133
BcgI CGANNNNNNTGC 2 cut(s) 1038, 1072
BciT130I CCWGG 3 cut(s) 202, 949, 2435
BciVI GTATCC 3 cut(s) 325, 545, 570
BclI TGATCA 1 cut(s) 2112
BcnI CCSGG 2 cut(s) 430, 517
BcoDI GTCTC 4 cut(s) 517, 1859, 1867, 2423
BcuI ACTAGT 1 cut(s) 2165
BfaI CTAG 4 cut(s) 141, 452, 816, 2166
BfuAI ACCTGC 2 cut(s) 783, 2235
BfuI GTATCC 3 cut(s) 325, 545, 570
BisI GCNGC 5 cut(s) 40, 232, 1242, 1545, 1855
BlsI GCNGC 5 cut(s) 41, 233, 1243, 1546, 1856
Bme1390I CCNGG 5 cut(s) 202, 430, 517, 949, 2435
Bme18I GGWCC 5 cut(s) 317, 923, 1304, 1762, 2215
BmeT110I CYCGRG 1 cut(s) 1216
BmgT120I GGNCC 8 cut(s) 317, 529, 857, 923, 1304, 1762, 2215, 2254
BmiI GGNNCC 9 cut(s) 199, 427, 435, 844, 1305, 1383, 1763, 1786, 2217
BmrFI CCNGG 5 cut(s) 202, 430, 517, 949, 2435
BmsI GCATC 4 cut(s) 485, 745, 1639, 2381
BpmI CTGGAG 2 cut(s) 29, 2028
BpuEI CTTGAG 2 cut(s) 745, 1098
BpuMI CCSGG 2 cut(s) 430, 517
Bsa29I ATCGAT 2 cut(s) 1058, 1957
BsaBI GATNNNNATC 1 cut(s) 156
BsaI GGTCTC 2 cut(s) 1867, 2423
BsaJI CCNNGG 6 cut(s) 313, 429, 948, 1765, 2410, 2433
BsaWI WCCGGW 2 cut(s) 116, 1384
BsaXI ACNNNNNCTCC 4 cut(s) 410, 440, 904, 934
Bsc4I CCNNNNNNNGG 2 cut(s) 1354, 2071
Bse118I RCCGGY 2 cut(s) 116, 845
Bse1I ACTGG 2 cut(s) 82, 176
Bse3DI GCAATG 1 cut(s) 134
Bse8I GATNNNNATC 1 cut(s) 156
BseBI CCWGG 3 cut(s) 202, 949, 2435
BseCI ATCGAT 2 cut(s) 1058, 1957
BseDI CCNNGG 6 cut(s) 313, 429, 948, 1765, 2410, 2433
BseGI GGATG 2 cut(s) 1527, 2256
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 2 cut(s) 1354, 2071
BseMI GCAATG 1 cut(s) 134
BseMII CTCAG 3 cut(s) 30, 472, 1719
BseNI ACTGG 2 cut(s) 82, 176
BseRI GAGGAG 3 cut(s) 858, 1849, 2087
BseSI GKGCMC 2 cut(s) 1111, 2044
BseXI GCAGC 2 cut(s) 1228, 1556
BseYI CCCAGC 1 cut(s) 1689
BsgI GTGCAG 2 cut(s) 293, 761
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 8 cut(s) 42, 282, 463, 531, 859, 1827, 2148, 2256
BshTI ACCGGT 1 cut(s) 116
BshVI ATCGAT 2 cut(s) 1058, 1957
BsiHKAI GWGCWC 2 cut(s) 375, 917
BsiHKCI CYCGRG 1 cut(s) 1216
BsiSI CCGG 5 cut(s) 117, 429, 517, 846, 1385
BslFI GGGAC 4 cut(s) 368, 851, 1317, 2201
BslI CCNNNNNNNGG 2 cut(s) 1354, 2071
BsmAI GTCTC 4 cut(s) 517, 1859, 1867, 2423
BsmFI GGGAC 4 cut(s) 368, 851, 1317, 2201
BsmI GAATGC 1 cut(s) 1987
BsnI GGCC 8 cut(s) 42, 282, 463, 531, 859, 1827, 2148, 2256
Bso31I GGTCTC 2 cut(s) 1867, 2423
BsoBI CYCGRG 1 cut(s) 1216
Bsp1286I GDGCHC 4 cut(s) 375, 917, 1111, 2044
Bsp143I GATC 8 cut(s) 246, 577, 1059, 1099, 1279, 1678, 1958, 2112
BspACI CCGC 7 cut(s) 39, 231, 1659, 1854, 2045, 2095, 2391
BspANI GGCC 8 cut(s) 42, 282, 463, 531, 859, 1827, 2148, 2256
BspCNI CTCAG 3 cut(s) 29, 471, 1720
BspDI ATCGAT 2 cut(s) 1058, 1957
BspFNI CGCG 1 cut(s) 231
BspLI GGNNCC 9 cut(s) 199, 427, 435, 844, 1305, 1383, 1763, 1786, 2217
BspMI ACCTGC 2 cut(s) 783, 2235
BspPI GGATC 5 cut(s) 241, 1094, 1287, 1673, 1953
BspTNI GGTCTC 2 cut(s) 1867, 2423
BsrDI GCAATG 1 cut(s) 134
BsrFI RCCGGY 2 cut(s) 116, 845
BsrI ACTGG 2 cut(s) 82, 176
BssAI RCCGGY 2 cut(s) 116, 845
BssECI CCNNGG 6 cut(s) 313, 429, 948, 1765, 2410, 2433
BssMI GATC 8 cut(s) 246, 577, 1059, 1099, 1279, 1678, 1958, 2112
BssSI CACGAG 2 cut(s) 918, 2156
BssT1I CCWWGG 3 cut(s) 313, 1765, 2410
Bst2BI CACGAG 2 cut(s) 918, 2156
Bst2UI CCWGG 3 cut(s) 202, 949, 2435
Bst6I CTCTTC 2 cut(s) 2064, 2380
BstAPI GCANNNNNTGC 1 cut(s) 36
BstC8I GCNNGC 2 cut(s) 847, 1985
BstDEI CTNAG 8 cut(s) 16, 344, 458, 954, 1427, 1534, 1728, 2258
BstEII GGTNACC 1 cut(s) 770
BstF5I GGATG 2 cut(s) 1527, 2256
BstFNI CGCG 1 cut(s) 231
BstHHI GCGC 1 cut(s) 690
BstKTI GATC 8 cut(s) 249, 580, 1062, 1102, 1282, 1681, 1961, 2115
BstMAI GTCTC 4 cut(s) 517, 1859, 1867, 2423
BstMBI GATC 8 cut(s) 246, 577, 1059, 1099, 1279, 1678, 1958, 2112
BstMWI GCNNNNNNNGC 3 cut(s) 36, 1560, 1644
BstNI CCWGG 3 cut(s) 202, 949, 2435
BstNSI RCATGY 3 cut(s) 407, 1463, 1807
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 5 cut(s) 200, 428, 515, 947, 2433
BstSLI GKGCMC 2 cut(s) 1111, 2044
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 1228, 1556
BstX2I RGATCY 2 cut(s) 1099, 1279
BstYI RGATCY 2 cut(s) 1099, 1279
Bsu15I ATCGAT 2 cut(s) 1058, 1957
BsuI GTATCC 3 cut(s) 325, 545, 570
BsuRI GGCC 8 cut(s) 42, 282, 463, 531, 859, 1827, 2148, 2256
BsuTUI ATCGAT 2 cut(s) 1058, 1957
BtsCI GGATG 2 cut(s) 1527, 2256
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 2 cut(s) 690, 1875
BveI ACCTGC 2 cut(s) 783, 2235
Cac8I GCNNGC 2 cut(s) 847, 1985
CfoI GCGC 1 cut(s) 690
Cfr10I RCCGGY 2 cut(s) 116, 845
Cfr13I GGNCC 8 cut(s) 317, 529, 857, 923, 1304, 1762, 2215, 2254
ClaI ATCGAT 2 cut(s) 1058, 1957
CseI GACGC 2 cut(s) 107, 1055
Csp6I GTAC 2 cut(s) 1605, 1652
CspAI ACCGGT 1 cut(s) 116
CviQI GTAC 2 cut(s) 1605, 1652
DdeI CTNAG 8 cut(s) 16, 344, 458, 954, 1427, 1534, 1728, 2258
DpnI GATC 8 cut(s) 248, 579, 1061, 1101, 1281, 1680, 1960, 2114
DpnII GATC 8 cut(s) 246, 577, 1059, 1099, 1279, 1678, 1958, 2112
EaeI YGGCCR 3 cut(s) 280, 1825, 2146
Eam1104I CTCTTC 2 cut(s) 2064, 2380
EarI CTCTTC 2 cut(s) 2064, 2380
EciI GGCGGA 2 cut(s) 2084, 2406
Ecl136II GAGCTC 1 cut(s) 373
Eco130I CCWWGG 3 cut(s) 313, 1765, 2410
Eco147I AGGCCT 1 cut(s) 463
Eco24I GRGCYC 1 cut(s) 375
Eco31I GGTCTC 2 cut(s) 1867, 2423
Eco32I GATATC 1 cut(s) 571
Eco47I GGWCC 5 cut(s) 317, 923, 1304, 1762, 2215
Eco53kI GAGCTC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 2195
Eco88I CYCGRG 1 cut(s) 1216
Eco91I GGTNACC 1 cut(s) 770
EcoICRI GAGCTC 1 cut(s) 373
EcoO109I RGGNCCY 2 cut(s) 1762, 2215
EcoO65I GGTNACC 1 cut(s) 770
EcoRII CCWGG 3 cut(s) 200, 947, 2433
EcoRV GATATC 1 cut(s) 571
EcoT14I CCWWGG 3 cut(s) 313, 1765, 2410
EcoT22I ATGCAT 1 cut(s) 478
EcoT38I GRGCYC 1 cut(s) 375
ErhI CCWWGG 3 cut(s) 313, 1765, 2410
FalI AAGNNNNNCTT 2 cut(s) 2394, 2426
FaqI GGGAC 4 cut(s) 368, 851, 1317, 2201
FauI CCCGC 1 cut(s) 2052
FauNDI CATATG 1 cut(s) 928
FbaI TGATCA 1 cut(s) 2112
FblI GTMKAC 1 cut(s) 2406
Fnu4HI GCNGC 5 cut(s) 40, 232, 1242, 1545, 1855
FokI GGATG 2 cut(s) 1514, 2263
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 5 cut(s) 40, 232, 1242, 1545, 1855
FspAI RTGCGCAY 1 cut(s) 689
FspBI CTAG 4 cut(s) 141, 452, 816, 2166
FspI TGCGCA 1 cut(s) 689
GlaI GCGC 1 cut(s) 689
GluI GCNGC 5 cut(s) 40, 232, 1242, 1545, 1855
GsaI CCCAGC 1 cut(s) 1693
GsuI CTGGAG 2 cut(s) 29, 2028
HaeIII GGCC 8 cut(s) 42, 282, 463, 531, 859, 1827, 2148, 2256
HapII CCGG 5 cut(s) 117, 429, 517, 846, 1385
HgaI GACGC 2 cut(s) 107, 1055
HhaI GCGC 1 cut(s) 690
Hin6I GCGC 1 cut(s) 688
HinP1I GCGC 1 cut(s) 688
HincII GTYRAC 4 cut(s) 882, 1045, 1068, 2327
HindII GTYRAC 4 cut(s) 882, 1045, 1068, 2327
HinfI GANTC 7 cut(s) 582, 650, 719, 1160, 1170, 1610, 2105
HpaII CCGG 5 cut(s) 117, 429, 517, 846, 1385
HphI GGTGA 5 cut(s) 131, 427, 937, 1155, 2188
Hpy188I TCNGA 7 cut(s) 67, 251, 355, 392, 649, 1729, 2344
Hpy99I CGWCG 1 cut(s) 2467
HpyAV CCTTC 5 cut(s) 115, 188, 977, 1675, 1972
HpyCH4IV ACGT 3 cut(s) 828, 1619, 1978
HpyCH4V TGCA 9 cut(s) 310, 476, 778, 785, 823, 1445, 1470, 2336, 2372
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 1560, 1644
HpyF3I CTNAG 8 cut(s) 16, 344, 458, 954, 1427, 1534, 1728, 2258
HpySE526I ACGT 3 cut(s) 828, 1619, 1978
HspAI GCGC 1 cut(s) 688
KroI GCCGGC 1 cut(s) 845
KroNI GCCGGC 1 cut(s) 847
Ksp22I TGATCA 1 cut(s) 2112
Kzo9I GATC 8 cut(s) 246, 577, 1059, 1099, 1279, 1678, 1958, 2112
LmnI GCTCC 6 cut(s) 183, 197, 431, 842, 871, 912
Lsp1109I GCAGC 2 cut(s) 1228, 1556
LweI GCATC 4 cut(s) 485, 745, 1639, 2381
MaeI CTAG 4 cut(s) 141, 452, 816, 2166
MaeII ACGT 3 cut(s) 828, 1619, 1978
MaeIII GTNAC 6 cut(s) 143, 223, 770, 1142, 1161, 1186
MalI GATC 8 cut(s) 248, 579, 1061, 1101, 1281, 1680, 1960, 2114
MboI GATC 8 cut(s) 246, 577, 1059, 1099, 1279, 1678, 1958, 2112
MboII GAAGA 8 cut(s) 1049, 1489, 1492, 1592, 2081, 2299, 2365, 2397
MfeI CAATTG 1 cut(s) 2379
MflI RGATCY 2 cut(s) 1099, 1279
MhlI GDGCHC 4 cut(s) 375, 917, 1111, 2044
MlsI TGGCCA 2 cut(s) 282, 1827
MluNI TGGCCA 2 cut(s) 282, 1827
MlyI GAGTC 4 cut(s) 644, 1169, 1619, 2099
MmeI TCCRAC 2 cut(s) 2016, 2157
Mox20I TGGCCA 2 cut(s) 282, 1827
Mph1103I ATGCAT 1 cut(s) 478
MroNI GCCGGC 1 cut(s) 845
MroXI GAANNNNTTC 1 cut(s) 2291
MscI TGGCCA 2 cut(s) 282, 1827
MseI TTAA 6 cut(s) 84, 1005, 1080, 1226, 1298, 2488
MslI CAYNNNNRTG 2 cut(s) 150, 2120
Msp20I TGGCCA 2 cut(s) 282, 1827
MspA1I CMGCKG 1 cut(s) 1693
MspI CCGG 5 cut(s) 117, 429, 517, 846, 1385
MspR9I CCNGG 5 cut(s) 202, 430, 517, 949, 2435
MunI CAATTG 1 cut(s) 2379
Mva1269I GAATGC 1 cut(s) 1987
MvaI CCWGG 3 cut(s) 202, 949, 2435
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 3 cut(s) 36, 1560, 1644
NaeI GCCGGC 1 cut(s) 847
NciI CCSGG 2 cut(s) 430, 517
NdeI CATATG 1 cut(s) 928
NdeII GATC 8 cut(s) 246, 577, 1059, 1099, 1279, 1678, 1958, 2112
NgoMIV GCCGGC 1 cut(s) 845
NlaIV GGNNCC 9 cut(s) 199, 427, 435, 844, 1305, 1383, 1763, 1786, 2217
NmuCI GTSAC 2 cut(s) 143, 1161
NsbI TGCGCA 1 cut(s) 689
NsiI ATGCAT 1 cut(s) 478
NspI RCATGY 3 cut(s) 407, 1463, 1807
PaeR7I CTCGAG 1 cut(s) 1216
PaqCI CACCTGC 1 cut(s) 2235
PceI AGGCCT 1 cut(s) 463
PciI ACATGT 2 cut(s) 403, 1459
PctI GAATGC 1 cut(s) 1987
PdiI GCCGGC 1 cut(s) 847
PdmI GAANNNNTTC 1 cut(s) 2291
PfeI GAWTC 3 cut(s) 582, 719, 1170
PinAI ACCGGT 1 cut(s) 116
PkrI GCNGC 5 cut(s) 41, 233, 1243, 1546, 1856
PleI GAGTC 4 cut(s) 644, 1168, 1618, 2099
PpsI GAGTC 4 cut(s) 644, 1168, 1618, 2099
PpuMI RGGWCCY 2 cut(s) 1762, 2215
PscI ACATGT 2 cut(s) 403, 1459
PsiI TTATAA 1 cut(s) 1074
Psp124BI GAGCTC 1 cut(s) 375
Psp5II RGGWCCY 2 cut(s) 1762, 2215
Psp6I CCWGG 3 cut(s) 200, 947, 2433
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 1 cut(s) 1689
PspGI CCWGG 3 cut(s) 200, 947, 2433
PspN4I GGNNCC 9 cut(s) 199, 427, 435, 844, 1305, 1383, 1763, 1786, 2217
PspPI GGNCC 8 cut(s) 317, 529, 857, 923, 1304, 1762, 2215, 2254
PspPPI RGGWCCY 2 cut(s) 1762, 2215
PspXI VCTCGAGB 1 cut(s) 1216
PsuI RGATCY 2 cut(s) 1099, 1279
PvuII CAGCTG 1 cut(s) 1693
RsaI GTAC 2 cut(s) 1606, 1653
RsaNI GTAC 2 cut(s) 1605, 1652
RseI CAYNNNNRTG 2 cut(s) 150, 2120
SacI GAGCTC 1 cut(s) 375
SaqAI TTAA 6 cut(s) 84, 1005, 1080, 1226, 1298, 2488
SatI GCNGC 5 cut(s) 40, 232, 1242, 1545, 1855
Sau3AI GATC 8 cut(s) 246, 577, 1059, 1099, 1279, 1678, 1958, 2112
Sau96I GGNCC 8 cut(s) 317, 529, 857, 923, 1304, 1762, 2215, 2254
SchI GAGTC 4 cut(s) 644, 1169, 1619, 2099
ScrFI CCNGG 5 cut(s) 202, 430, 517, 949, 2435
SduI GDGCHC 4 cut(s) 375, 917, 1111, 2044
SfaNI GCATC 4 cut(s) 485, 745, 1639, 2381
Sfr274I CTCGAG 1 cut(s) 1216
SgrAI CRCCGGYG 1 cut(s) 116
SinI GGWCC 5 cut(s) 317, 923, 1304, 1762, 2215
SlaI CTCGAG 1 cut(s) 1216
SmiMI CAYNNNNRTG 2 cut(s) 150, 2120
SmlI CTYRAG 3 cut(s) 724, 1113, 1216
SmoI CTYRAG 3 cut(s) 724, 1113, 1216
SpeI ACTAGT 1 cut(s) 2165
SseBI AGGCCT 1 cut(s) 463
SsiI CCGC 7 cut(s) 39, 231, 1659, 1854, 2045, 2095, 2391
SspI AATATT 3 cut(s) 242, 764, 1078
SspMI CTAG 4 cut(s) 141, 452, 816, 2166
SstI GAGCTC 1 cut(s) 375
StuI AGGCCT 1 cut(s) 463
StyD4I CCNGG 5 cut(s) 200, 428, 515, 947, 2433
StyI CCWWGG 3 cut(s) 313, 1765, 2410
TaiI ACGT 3 cut(s) 831, 1622, 1981
TaqII GACCGA 1 cut(s) 1992
TauI GCSGC 3 cut(s) 42, 234, 1857
TfiI GAWTC 3 cut(s) 582, 719, 1170
Tru1I TTAA 6 cut(s) 84, 1005, 1080, 1226, 1298, 2488
Tru9I TTAA 6 cut(s) 84, 1005, 1080, 1226, 1298, 2488
TscAI CASTG 2 cut(s) 697, 1875
TseFI GTSAC 2 cut(s) 143, 1161
TseI GCWGC 2 cut(s) 1241, 1544
Tsp45I GTSAC 2 cut(s) 143, 1161
TspDTI ATGAA 6 cut(s) 13, 97, 1516, 1599, 2300, 2448
TspGWI ACGGA 1 cut(s) 855
TspRI CASTG 2 cut(s) 697, 1875
VpaK11BI GGWCC 5 cut(s) 317, 923, 1304, 1762, 2215
XapI RAATTY 9 cut(s) 128, 299, 1154, 1316, 1328, 1336, 1521, 2290, 2483
XbaI TCTAGA 2 cut(s) 451, 815
XceI RCATGY 3 cut(s) 407, 1463, 1807
XhoI CTCGAG 1 cut(s) 1216
XmiI GTMKAC 1 cut(s) 2406
XmnI GAANNNNTTC 1 cut(s) 2291
XspI CTAG 4 cut(s) 141, 452, 816, 2166
Zsp2I ATGCAT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.