FvH4_6g32660

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000083
Physical Location & Seq
Reverse (-)
25694133 .. 25701893
7761 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32660.t1

Sequence Viewer

Length: 2751 bp
ATGTCGCGGGCATTCAAGCAATTCTTCTTTGCAGTACTCGGTTGTTTCTCTCTTATGCTCTTTGTTCATGCCCAGGATCAATCAGGTTACATTAGCATAGATTGTGGGGGAACGCACATATCTAGCTATAACGAGGAGACAACTGGCCTCACCTTCATTTCAGACAAAGATTTCATTGACACGGGTGAATCAAAAATGGTGTCGCCTGAATACAGAGGCATTTGGCAGCAACCGTACGACTCTCTAAGGAGCTTTCCCAAAGGAACTAGGAACTGCTACAGACTGAACCTTACAAGTAATTCCAAATATCTGGTTCGAGCAACTTTCTATTATGGGAATTATGATGGCCAAAATAAGTTACCGGAGTTCGAATTACATCTCGGACCTAATTTGTGGCAATCAGTGACGTTCCAGGATGCATCAAGTTATATCAACAATGAGCTCATACATATCCCGCTCCGAGACCATGTACATGTTTGTCTTGTGAACACAGGCTCCGGGGTTCCCTTCATATCAACGATAGAGATACGGCTGTTTCCTAATTCCACCTTTGAAGAAGAACTCGTGGACGAACAATCCTTGGCACTTGTCTCCAGGTTTGACACCGGTCAAATAGCACCTCGTACAGCATACAGGTATCCTTCTGATGAACTCGATCGCATCTGGTATTCCTACAATGATTGGGCTGATTGGACACAGTTAACCGCCTCATCCAACGTCGACACATCAGGCGATTATTACGAAATACCTTCTGTTGTTATGAACACTGCTGCCACGCCAAGAAAGGAAACTGATTCCTTTATTAATATCTGGCTGCCTGAGTCTGATGATGATACGACCCTGTACTATATTTACTTGCACTTTGCTGAAGTTGAAAAGCTCCAGCCAAACCAGTCTAGATGGTTCAATATCACCTCTAGTGGAGGAAACTTTGCGTCATATTCTCCTGAGTATGGTAAGAATGCTATTTATTTCAACTCCAAGCCTCTCACACGAAACAAAATAAATGTTTCAGTTGTTCTGATGGAAAACTCGACCCTTCCACCAATCCTGAATGCCTTTGAGGTTTATATGATAACAAAACTGGCAGGCTCAGAAACAAATGAAAAGGATTTGAACACAATCACAAACATCAGGAATACTTATAGAATTGGGAAGAATTGGCAAGGAGATCCATGTGCTCCTCAAAAGTACTCGTGGGAAGGTCTAAACTGCACCTATCCTGAAAATAACGAGCCCTCAAGAATAATATCATTGGACTTGTCCTCGAGTGGATTGACAGAGGAGATAACTCCGTGGATATCAGACCTTGCAATGTTACAGACATTGAATTTGTCGAACAACAACTTAACTGGTTCGCTTCCAAGATTTTTATCTCAGCTGCAAAATTTACGTGTCCTGGATCTATCAAACAATAACTTCACAGGATCAATTCCAAACATTTTGTCTCAATTGCCAAACTTAAATGTCCTATACTTGGAGGACAACATGCTCACAGGATCAGTTCCAGAAGGCTTGATGTTGAAAAAAAGTAGCGGTTTTCTATCATTACGGGTGTGCAACAACCCCAATCTCTCTGGAGCTTTTTCTTGCGAGGAGGAAGATGAGAAAAAGAAGAAGCCCAATTTTTTCCTTCTTGTAGGTTTACCAGTAATCGGAGTAGCTTCGATTATTTTCCTAATTGTAGCAGCTATTTGCGCCTGCAAAAGGAAAAGCAAAGATGGAGCTGCCGTAGGGGAACAACCCCGCAATGGGTTAGTGGAACTTGAGCCAACACGACGACAGTTCACATACTCAGATATCCTCAAGATCACTAACAATAGACAAAGGATTCTCGGCAAAGGTGGATTTGGAACTGTTTATCATGGCTACATCGATGACAATACTCAAGTAGCTGTCAAGCTGCTTTCGTCATCATCAAGTCGAGGGCTTGAACAATTCCACGCAGAGGTTAATCTTCTGATGAGGGTTCATCATACAAACTTGACTAGCCTTGTAGGATATTGTGATGATGAAGACAACAAAGGACTCGTTTATGAGTACATGGCCAGGGGAAACTTGCAAGAATATCTTTCAGATAAAAGTTCAAATGTCCTGACTTGGGAAGGTAGACTTTATATAGCAACAGATACTGCACAAGGATTGGAGTATCTACACTATGGTTGTAAACCTCCTATGATCCATAGGGATGTCAAGACAACAAACATCTTGATATCAGAAAATTTCCAAGCCAAACTCTCCGATTTCGGCCTCTCTAGAAATTTCCCTGCAGGCGATGGGACTCATATAGTTACAGGTGTTGCTGGCACCCCTGGCTACCTAGCACCCGAGTACAAACAACTAAACAGATTCAACGAGAAGAGTGATGTCTATAGCTTTGGGATTGTACTGCTGGAGATTATCACAGGTCAACCTGCTGTTTCGAGGACACGAGAGAGAATTGCCATCAATGAATGGGTTGGATCGATCATTCCAAAAGGAGACATTGACACTATTGTGGATCCAAGGTTGAAAGGAATCTTCAATGTTAGCTCTGCGTGGAAAGTAGTGGAAATAGCAATGGCATGTGTCTCTCCAGAGGCCAATAAAAGGCCAACCATGAGTAAAGTTGTTGGAGAACTGAAGCAGTGTCTGGAAACACAACTGTCTCAAAGAAACCAGAGCAGCTATGGATCTGAACTTGGGAATGCCTTTGACATTGTGTCTGAGAATGGCTATTCGATCACTATGCTACGTCCCTCAGTTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

917

Amino Acids

102.87

Weight (kDa)

5.38

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 33 - 358 2.8e-100 Malectin-like domain
LRR_8 PF13855 418 - 474 2.1e-06 Leucine rich repeat
LRR_8 PF13855 440 - 498 1.3e-08 Leucine rich repeat
LRR_4 PF12799 440 - 479 7.5e-07 Leucine Rich repeats (2 copies)
LRR_4 PF12799 462 - 496 1.8e-06 Leucine Rich repeats (2 copies)
Pkinase PF00069 608 - 871 3.4e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 609 - 873 4.4e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000083)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07560 AT1G51790 AT1G51790 AT1G51790 AT1G51800 AT1G51805 AT1G51805 AT1G51810 AT1G51820 AT1G51820 AT1G51830 AT1G51830 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51850 AT1G51850 AT1G51860 AT1G51860 AT1G51870 AT1G51870 AT1G51880 AT1G51880 AT1G51880 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51910 AT2G04300 AT2G04300 AT2G28960 AT2G28960 AT2G28970 AT2G28970 AT2G28990 AT2G28990 AT2G29000 AT3G21340 AT3G46350 AT5G59616 AT5G59650 AT5G59650 AT5G59660 AT5G59660 AT5G59660 AT5G59670 AT5G59670 AT5G59680
fragaria_vesca FvH4_2g03270 FvH4_3g21300 FvH4_6g32660 FvH4_6g32660 FvH4_6g32670 FvH4_6g32670 FvH4_6g32690 FvH4_6g32690 FvH4_6g32691 FvH4_6g32700 FvH4_6g32740 FvH4_6g32750 FvH4_6g33210 FvH4_6g33210 FvH4_7g24270 FvH4_7g24280 FvH4_7g24291 FvH4_7g24300
malus_domestica MD09G1198400.v1.1 MD09G1198600.v1.1 MD09G1199100.v1.1 MD09G1199200.v1.1 MD09G1199300.v1.1 MD09G1199500.v1.1 MD09G1199600.v1.1 MD17G1179300.v1.1 MD17G1180200.v1.1 MD17G1266000.v1.1
prunus_persica Prupe.1G438900_v2.0.a1 Prupe.1G439000_v2.0.a1 Prupe.1G439100_v2.0.a1 Prupe.3G019400_v2.0.a1 Prupe.3G021900_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G055100_v2.0.a1 Prupe.3G055400_v2.0.a1 Prupe.3G055500_v2.0.a1 Prupe.3G055600_v2.0.a1 Prupe.3G055700_v2.0.a1 Prupe.3G055800_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056100_v2.0.a1 Prupe.3G209600_v2.0.a1 Prupe.3G209700_v2.0.a1 Prupe.6G291100_v2.0.a1 Prupe.7G208900_v2.0.a1 Prupe.8G038300_v2.0.a1
pyrus_communis pycom09g11570 pycom09g11580 pycom09g11610 pycom09g11630 pycom09g11640 pycom17g18840 pycom17g18910 pycom17g18930 pycom17g18940
rosa_chinensis RchiOBHm_Chr1g0317521 RchiOBHm_Chr1g0317541 RchiOBHm_Chr2g0123351 RchiOBHm_Chr2g0141181 RchiOBHm_Chr2g0141191 RchiOBHm_Chr2g0141211 RchiOBHm_Chr2g0141221 RchiOBHm_Chr2g0141261 RchiOBHm_Chr2g0141271 RchiOBHm_Chr2g0141311 RchiOBHm_Chr2g0141331 RchiOBHm_Chr2g0141371 RchiOBHm_Chr2g0141391 RchiOBHm_Chr2g0141401 RchiOBHm_Chr2g0141431 RchiOBHm_Chr2g0141481 RchiOBHm_Chr2g0141491 RchiOBHm_Chr2g0141591 RchiOBHm_Chr2g0141601 RchiOBHm_Chr2g0141611 RchiOBHm_Chr2g0141631 RchiOBHm_Chr2g0141641 RchiOBHm_Chr2g0141661 RchiOBHm_Chr2g0141681 RchiOBHm_Chr2g0141701 RchiOBHm_Chr2g0141781 RchiOBHm_Chr2g0141811 RchiOBHm_Chr2g0141821 RchiOBHm_Chr3g0479311 RchiOBHm_Chr3g0495751 RchiOBHm_Chr4g0404741 RchiOBHm_Chr4g0410341 RchiOBHm_Chr4g0418371 RchiOBHm_Chr4g0438651 RchiOBHm_Chr5g0036411 RchiOBHm_Chr5g0036491 RchiOBHm_Chr5g0042391 RchiOBHm_Chr6g0279871 RchiOBHm_Chr7g0192921 RchiOBHm_Chr7g0214901 RchiOBHm_Chr7g0234931
rosa_laevigata RLG00000003432 RLG00000008806 RLG00000013062 RLG00000019514 RLG00000019923 RLG00000019924 RLG00000019925 RLG00000019930 RLG00000019931 RLG00000019934 RLG00000019937 RLG00000019938 RLG00000033697
rosa_multiflora Rmu_co8002302.1_g000001 Rmu_sc0000147.1_g000031 Rmu_sc0000463.1_g000005 Rmu_sc0000712.1_g000003 Rmu_sc0001483.1_g000017 Rmu_sc0001554.1_g000002 Rmu_sc0001554.1_g000005 Rmu_sc0001554.1_g000008 Rmu_sc0001981.1_g000017 Rmu_sc0002040.1_g000056 Rmu_sc0002755.1_g000018 Rmu_sc0002804.1_g000016 Rmu_sc0002928.1_g000015 Rmu_sc0003040.1_g000008 Rmu_sc0003040.1_g000018 Rmu_sc0004512.1_g000001 Rmu_sc0004512.1_g000004 Rmu_sc0005507.1_g000001 Rmu_sc0005507.1_g000009 Rmu_sc0005507.1_g000033 Rmu_sc0005507.1_g000037 Rmu_sc0005507.1_g000043 Rmu_sc0005507.1_g000047 Rmu_sc0006318.1_g000004 Rmu_sc0006318.1_g000014 Rmu_sc0006318.1_g000021 Rmu_sc0006556.1_g000001 Rmu_sc0007208.1_g000002 Rmu_sc0007208.1_g000003 Rmu_sc0007208.1_g000006 Rmu_sc0007208.1_g000008
rosa_roxburghii Rroxscaffold_1G00044430 Rroxscaffold_2G00103420 Rroxscaffold_2G00103470 Rroxscaffold_2G00103490 Rroxscaffold_2G00103500 Rroxscaffold_2G00103520 Rroxscaffold_2G00103530 Rroxscaffold_2G00103540 Rroxscaffold_2G00103550 Rroxscaffold_2G00103570 Rroxscaffold_2G00103770 Rroxscaffold_2G00103780 Rroxscaffold_2G00103800 Rroxscaffold_2G00103820 Rroxscaffold_2G00103860 Rroxscaffold_2G00103890 Rroxscaffold_2G00103930 Rroxscaffold_2G00103940 Rroxscaffold_2G00104010 Rroxscaffold_2G00104030 Rroxscaffold_2G00104050 Rroxscaffold_2G00104060 Rroxscaffold_2G00104070 Rroxscaffold_2G00104090 Rroxscaffold_2G00104100 Rroxscaffold_2G00104120 Rroxscaffold_2G00104130 Rroxscaffold_4G00331710
rosa_rugosa Rorug02G0367500 Rorug02G0367500 Rorug02G0367600 Rorug02G0367800 Rorug02G0367800 Rorug02G0367900 Rorug02G0368100 Rorug02G0368100 Rorug02G0368100 Rorug02G0368200 Rorug02G0368700 Rorug02G0368900 Rorug05G0155600 Rorug05G0155700 Rorug05G0155800 Rorug05G0156700 Rorug05G0157300
rosa_samantha Rh1AG028000 Rh2AG418700 Rh2AG418900 Rh2AG419000 Rh2AG419100 Rh2AG419400 Rh2AG419600 Rh2AG419800 Rh2AG420400 Rh2AG421000 Rh2BG428500 Rh2BG428600 Rh2BG428800 Rh2BG428900 Rh2BG429300 Rh2BG429400 Rh2BG429700 Rh2BG429900 Rh2BG430000 Rh2BG430700 Rh2CG405100 Rh2CG405200 Rh2CG405300 Rh2CG405500 Rh2CG405600 Rh2CG405700 Rh2CG406000 Rh2CG406100 Rh2CG406300 Rh2CG406600 Rh2CG406700 Rh2CG407500 Rh2CG408100 Rh2DG438500 Rh3DG123900 Rh3DG124000 Rh5BG249700 Rh5BG482500 Rh5CG281500 Rh5CG507300 Rh7DG133300
rosa_wichuraiana Rw0G019720 Rw0G019730 Rw0G019750 Rw0G023010 Rw2G034340 Rw2G034350 Rw2G034370 Rw2G034380 Rw2G034400 Rw2G034430 Rw2G034470 Rw2G034480 Rw2G034510 Rw5G022890 Rw5G026410 Rw5G032710 Rw5G042850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2422
AccB1I GGYRCC 1 cut(s) 2306
AccBSI CCGCTC 1 cut(s) 457
AccI GTMKAC 2 cut(s) 720, 2110
AccII CGCG 1 cut(s) 7
AciI CCGC 5 cut(s) 7, 455, 705, 1536, 1747
AcoI YGGCCR 2 cut(s) 346, 2046
AcsI RAATTY 4 cut(s) 1330, 1387, 2221, 2260
AcuI CTGAAG 2 cut(s) 888, 2642
AfaI GTAC 9 cut(s) 36, 236, 471, 625, 845, 1193, 2042, 2333, 2388
AfiI CCNNNNNNNGG 9 cut(s) 953, 1477, 1655, 1707, 1751, 1752, 1948, 2101, 2589
AflIII ACRYGT 2 cut(s) 472, 1393
AgeI ACCGGT 1 cut(s) 605
AhdI GACNNNNNGTC 1 cut(s) 2701
AjnI CCWGG 6 cut(s) 72, 411, 593, 1398, 2048, 2311
AleI CACNNNNGTG 1 cut(s) 2495
AloI GAACNNNNNNTCC 2 cut(s) 479, 511
Alw21I GWGCWC 2 cut(s) 444, 1183
Alw26I GTCTC 7 cut(s) 131, 456, 595, 1452, 2475, 2575, 2652
AlwNI CAGNNNCTG 2 cut(s) 2132, 2632
Ama87I CYCGRG 2 cut(s) 1267, 2327
AoxI GGCC 6 cut(s) 145, 346, 2046, 2248, 2580, 2591
ApeKI GCWGC 8 cut(s) 226, 770, 814, 1381, 1688, 1727, 1903, 2664
ApoI RAATTY 4 cut(s) 1330, 1387, 2221, 2260
ArsI GACNNNNNNTTYG 6 cut(s) 185, 217, 1315, 1347, 1430, 1462
AseI ATTAAT 1 cut(s) 804
AsiGI ACCGGT 1 cut(s) 605
AspLEI GCGC 1 cut(s) 1700
AspS9I GGNCC 1 cut(s) 383
AsuC2I CCSGG 1 cut(s) 499
AsuHPI GGTGA 3 cut(s) 142, 197, 904
AsuII TTCGAA 1 cut(s) 369
AvaI CYCGRG 2 cut(s) 1267, 2327
AvaII GGWCC 1 cut(s) 383
BaeI ACNNNNGTAYC 2 cut(s) 518, 551
BalI TGGCCA 2 cut(s) 348, 2048
BamHI GGATCC 1 cut(s) 2500
BanI GGYRCC 1 cut(s) 2306
BanII GRGCYC 2 cut(s) 444, 1239
BauI CACGAG 3 cut(s) 563, 1195, 2430
BbsI GAAGAC 1 cut(s) 2022
Bbv12I GWGCWC 2 cut(s) 444, 1183
BbvI GCAGC 8 cut(s) 238, 757, 801, 1368, 1700, 1714, 1890, 2676
BccI CCATC 6 cut(s) 338, 894, 1018, 1715, 2270, 2453
BceAI ACGGC 2 cut(s) 545, 1715
BcgI CGANNNNNNTGC 2 cut(s) 2710, 2744
BciT130I CCWGG 6 cut(s) 74, 413, 595, 1400, 2050, 2313
BciVI GTATCC 1 cut(s) 648
BcnI CCSGG 1 cut(s) 499
BcoDI GTCTC 7 cut(s) 131, 456, 595, 1452, 2475, 2575, 2652
BfaI CTAG 7 cut(s) 123, 267, 897, 918, 1989, 2256, 2321
BfmI CTRYAG 3 cut(s) 277, 2268, 2371
BfuAI ACCTGC 1 cut(s) 2422
BfuI GTATCC 1 cut(s) 648
BisI GCNGC 8 cut(s) 227, 771, 815, 1382, 1689, 1728, 1904, 2665
BlsI GCNGC 8 cut(s) 228, 772, 816, 1383, 1690, 1729, 1905, 2666
BmcAI AGTACT 2 cut(s) 36, 1193
Bme1390I CCNGG 7 cut(s) 74, 413, 499, 595, 1400, 2050, 2313
Bme18I GGWCC 1 cut(s) 383
BmeRI GACNNNNNGTC 1 cut(s) 2701
BmeT110I CYCGRG 2 cut(s) 1267, 2327
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 4 cut(s) 496, 504, 2308, 2502
BmrFI CCNGG 7 cut(s) 74, 413, 499, 595, 1400, 2050, 2313
BmsI GCATC 3 cut(s) 406, 428, 669
BoxI GACNNNNGTC 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 2022
BpmI CTGGAG 5 cut(s) 577, 866, 1599, 2414, 2559
Bpu14I TTCGAA 1 cut(s) 369
BpuEI CTTGAG 4 cut(s) 1225, 1787, 1790, 1872
BpuMI CCSGG 1 cut(s) 499
Bsa29I ATCGAT 2 cut(s) 1875, 2465
BsaAI YACGTR 1 cut(s) 1394
BsaBI GATNNNNATC 1 cut(s) 1372
BsaI GGTCTC 1 cut(s) 456
BsaJI CCNNGG 7 cut(s) 72, 498, 579, 1295, 2049, 2311, 2504
BsaWI WCCGGW 2 cut(s) 361, 605
BsaXI ACNNNNNCTCC 4 cut(s) 479, 509, 1716, 1746
Bsc4I CCNNNNNNNGG 9 cut(s) 953, 1477, 1655, 1707, 1751, 1752, 1948, 2101, 2589
Bse118I RCCGGY 1 cut(s) 605
Bse1I ACTGG 5 cut(s) 148, 892, 1089, 1357, 1649
Bse3DI GCAATG 3 cut(s) 1320, 1756, 2565
Bse8I GATNNNNATC 1 cut(s) 1372
BseBI CCWGG 6 cut(s) 74, 413, 595, 1400, 2050, 2313
BseCI ATCGAT 2 cut(s) 1875, 2465
BseDI CCNNGG 7 cut(s) 72, 498, 579, 1295, 2049, 2311, 2504
BseGI GGATG 3 cut(s) 421, 710, 2194
BseJI GATNNNNATC 1 cut(s) 1372
BseLI CCNNNNNNNGG 9 cut(s) 953, 1477, 1655, 1707, 1751, 1752, 1948, 2101, 2589
BseMI GCAATG 3 cut(s) 1320, 1756, 2565
BseMII CTCAG 6 cut(s) 810, 939, 1107, 1391, 1809, 2697
BseNI ACTGG 5 cut(s) 148, 892, 1089, 1357, 1649
BseRI GAGGAG 4 cut(s) 149, 1173, 1298, 1610
BseXI GCAGC 8 cut(s) 238, 757, 801, 1368, 1700, 1714, 1890, 2676
BsgI GTGCAG 2 cut(s) 1198, 2118
Bsh1236I CGCG 1 cut(s) 7
Bsh1285I CGRYCG 1 cut(s) 658
BshFI GGCC 6 cut(s) 147, 348, 2048, 2250, 2582, 2593
BshNI GGYRCC 1 cut(s) 2306
BshTI ACCGGT 1 cut(s) 605
BshVI ATCGAT 2 cut(s) 1875, 2465
BsiEI CGRYCG 1 cut(s) 658
BsiHKAI GWGCWC 2 cut(s) 444, 1183
BsiHKCI CYCGRG 2 cut(s) 1267, 2327
BsiSI CCGG 3 cut(s) 362, 498, 606
BsiWI CGTACG 1 cut(s) 234
BslFI GGGAC 2 cut(s) 2293, 2721
BslI CCNNNNNNNGG 9 cut(s) 953, 1477, 1655, 1707, 1751, 1752, 1948, 2101, 2589
BsmAI GTCTC 7 cut(s) 131, 456, 595, 1452, 2475, 2575, 2652
BsmFI GGGAC 2 cut(s) 2293, 2721
BsmI GAATGC 4 cut(s) 11, 967, 1060, 2692
BsnI GGCC 6 cut(s) 147, 348, 2048, 2250, 2582, 2593
Bso31I GGTCTC 1 cut(s) 456
BsoBI CYCGRG 2 cut(s) 1267, 2327
Bsp119I TTCGAA 1 cut(s) 369
Bsp1286I GDGCHC 3 cut(s) 444, 1183, 1239
Bsp1407I TGTACA 1 cut(s) 469
BspACI CCGC 5 cut(s) 7, 455, 705, 1536, 1747
BspANI GGCC 6 cut(s) 147, 348, 2048, 2250, 2582, 2593
BspCNI CTCAG 6 cut(s) 811, 940, 1106, 1390, 1808, 2698
BspDI ATCGAT 2 cut(s) 1875, 2465
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 4 cut(s) 496, 504, 2308, 2502
BspMAI CTGCAG 1 cut(s) 2272
BspMI ACCTGC 1 cut(s) 2422
BspT104I TTCGAA 1 cut(s) 369
BspT107I GGYRCC 1 cut(s) 2306
BspTNI GGTCTC 1 cut(s) 456
BsrBI CCGCTC 1 cut(s) 457
BsrDI GCAATG 3 cut(s) 1320, 1756, 2565
BsrFI RCCGGY 1 cut(s) 605
BsrGI TGTACA 1 cut(s) 469
BsrI ACTGG 5 cut(s) 148, 892, 1089, 1357, 1649
BssAI RCCGGY 1 cut(s) 605
BssECI CCNNGG 7 cut(s) 72, 498, 579, 1295, 2049, 2311, 2504
BssSI CACGAG 3 cut(s) 563, 1195, 2430
BssT1I CCWWGG 2 cut(s) 579, 2504
Bst2BI CACGAG 3 cut(s) 563, 1195, 2430
Bst2UI CCWGG 6 cut(s) 74, 413, 595, 1400, 2050, 2313
Bst4CI ACNGT 5 cut(s) 234, 699, 1785, 1858, 2646
Bst6I CTCTTC 1 cut(s) 2354
BstAUI TGTACA 1 cut(s) 469
BstBAI YACGTR 1 cut(s) 1394
BstBI TTCGAA 1 cut(s) 369
BstC8I GCNNGC 5 cut(s) 9, 1090, 1702, 2272, 2305
BstDEI CTNAG 8 cut(s) 245, 819, 948, 1093, 1377, 1795, 2706, 2740
BstDSI CCRYGG 1 cut(s) 1295
BstENI CCTNNNNNAGG 1 cut(s) 1705
BstF5I GGATG 3 cut(s) 421, 710, 2194
BstFNI CGCG 1 cut(s) 7
BstHHI GCGC 1 cut(s) 1700
BstMAI GTCTC 7 cut(s) 131, 456, 595, 1452, 2475, 2575, 2652
BstMCI CGRYCG 1 cut(s) 658
BstMWI GCNNNNNNNGC 2 cut(s) 1697, 2313
BstNI CCWGG 6 cut(s) 74, 413, 595, 1400, 2050, 2313
BstNSI RCATGY 3 cut(s) 476, 1492, 2568
BstPAI GACNNNNGTC 1 cut(s) 606
BstSCI CCNGG 7 cut(s) 72, 411, 497, 593, 1398, 2048, 2311
BstSFI CTRYAG 3 cut(s) 277, 2268, 2371
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 8 cut(s) 238, 757, 801, 1368, 1700, 1714, 1890, 2676
BstV2I GAAGAC 1 cut(s) 2022
BstX2I RGATCY 4 cut(s) 1171, 1402, 2500, 2672
BstXI CCANNNNNNTGG 1 cut(s) 310
BstYI RGATCY 4 cut(s) 1171, 1402, 2500, 2672
Bsu15I ATCGAT 2 cut(s) 1875, 2465
BsuI GTATCC 1 cut(s) 648
BsuRI GGCC 6 cut(s) 147, 348, 2048, 2250, 2582, 2593
BsuTUI ATCGAT 2 cut(s) 1875, 2465
BtgI CCRYGG 1 cut(s) 1295
BtgZI GCGATG 1 cut(s) 2289
BtsCI GGATG 3 cut(s) 421, 710, 2194
BtsI GCAGTG 2 cut(s) 765, 2633
BtsIMutI CAGTG 3 cut(s) 408, 765, 2633
BveI ACCTGC 1 cut(s) 2422
Cac8I GCNNGC 5 cut(s) 9, 1090, 1702, 2272, 2305
CaiI CAGNNNCTG 2 cut(s) 2132, 2632
CfoI GCGC 1 cut(s) 1700
Cfr10I RCCGGY 1 cut(s) 605
Cfr13I GGNCC 1 cut(s) 383
ClaI ATCGAT 2 cut(s) 1875, 2465
CseI GACGC 1 cut(s) 924
Csp6I GTAC 9 cut(s) 35, 235, 470, 624, 844, 1192, 2041, 2332, 2387
CspAI ACCGGT 1 cut(s) 605
CviAII CATG 9 cut(s) 68, 467, 473, 1176, 1489, 1865, 2044, 2565, 2599
CviQI GTAC 9 cut(s) 35, 235, 470, 624, 844, 1192, 2041, 2332, 2387
DdeI CTNAG 8 cut(s) 245, 819, 948, 1093, 1377, 1795, 2706, 2740
DriI GACNNNNNGTC 1 cut(s) 2701
EaeI YGGCCR 2 cut(s) 346, 2046
Eam1104I CTCTTC 1 cut(s) 2354
Eam1105I GACNNNNNGTC 1 cut(s) 2701
EarI CTCTTC 1 cut(s) 2354
Ecl136II GAGCTC 1 cut(s) 442
Eco130I CCWWGG 2 cut(s) 579, 2504
Eco24I GRGCYC 2 cut(s) 444, 1239
Eco31I GGTCTC 1 cut(s) 456
Eco32I GATATC 3 cut(s) 1302, 1801, 2214
Eco47I GGWCC 1 cut(s) 383
Eco53kI GAGCTC 1 cut(s) 442
Eco57I CTGAAG 2 cut(s) 888, 2642
Eco88I CYCGRG 2 cut(s) 1267, 2327
EcoICRI GAGCTC 1 cut(s) 442
EcoNI CCTNNNNNAGG 1 cut(s) 1705
EcoRII CCWGG 6 cut(s) 72, 411, 593, 1398, 2048, 2311
EcoRV GATATC 3 cut(s) 1302, 1801, 2214
EcoT14I CCWWGG 2 cut(s) 579, 2504
EcoT22I ATGCAT 1 cut(s) 421
EcoT38I GRGCYC 2 cut(s) 444, 1239
ErhI CCWWGG 2 cut(s) 579, 2504
FaeI CATG 9 cut(s) 71, 470, 476, 1179, 1492, 1868, 2047, 2568, 2602
FalI AAGNNNNNCTT 6 cut(s) 8, 40, 2055, 2087, 2097, 2129
FaqI GGGAC 2 cut(s) 2293, 2721
FatI CATG 9 cut(s) 67, 466, 472, 1175, 1488, 1864, 2043, 2564, 2598
FauI CCCGC 2 cut(s) 462, 1754
FblI GTMKAC 2 cut(s) 720, 2110
Fnu4HI GCNGC 8 cut(s) 227, 771, 815, 1382, 1689, 1728, 1904, 2665
FokI GGATG 3 cut(s) 428, 697, 2201
FriOI GRGCYC 2 cut(s) 444, 1239
Fsp4HI GCNGC 8 cut(s) 227, 771, 815, 1382, 1689, 1728, 1904, 2665
FspBI CTAG 7 cut(s) 123, 267, 897, 918, 1989, 2256, 2321
GlaI GCGC 1 cut(s) 1699
GluI GCNGC 8 cut(s) 227, 771, 815, 1382, 1689, 1728, 1904, 2665
GsuI CTGGAG 5 cut(s) 577, 866, 1599, 2414, 2559
HaeIII GGCC 6 cut(s) 147, 348, 2048, 2250, 2582, 2593
HapII CCGG 3 cut(s) 362, 498, 606
HgaI GACGC 1 cut(s) 924
HhaI GCGC 1 cut(s) 1700
Hin1II CATG 9 cut(s) 71, 470, 476, 1179, 1492, 1868, 2047, 2568, 2602
Hin6I GCGC 1 cut(s) 1698
HinP1I GCGC 1 cut(s) 1698
HincII GTYRAC 3 cut(s) 702, 721, 2411
HindII GTYRAC 3 cut(s) 702, 721, 2411
HinfI GANTC 9 cut(s) 188, 239, 794, 821, 1831, 2028, 2281, 2349, 2517
HpaI GTTAAC 1 cut(s) 702
HpaII CCGG 3 cut(s) 362, 498, 606
HphI GGTGA 3 cut(s) 142, 197, 904
Hpy166II GTNNAC 9 cut(s) 487, 568, 702, 721, 1646, 1788, 2111, 2168, 2411
Hpy8I GTNNAC 9 cut(s) 487, 568, 702, 721, 1646, 1788, 2111, 2168, 2411
Hpy99I CGWCG 2 cut(s) 722, 1782
HpyAV CCTTC 9 cut(s) 163, 517, 651, 759, 1049, 1196, 1505, 1643, 2099
HpyCH4III ACNGT 5 cut(s) 234, 699, 1785, 1858, 2646
HpyCH4IV ACGT 4 cut(s) 407, 717, 1393, 2734
HpyF10VI GCNNNNNNNGC 2 cut(s) 1697, 2313
HpyF3I CTNAG 8 cut(s) 245, 819, 948, 1093, 1377, 1795, 2706, 2740
HpySE526I ACGT 4 cut(s) 407, 717, 1393, 2734
Hsp92II CATG 9 cut(s) 71, 470, 476, 1179, 1492, 1868, 2047, 2568, 2602
HspAI GCGC 1 cut(s) 1698
KspAI GTTAAC 1 cut(s) 702
LmnI GCTCC 7 cut(s) 249, 462, 500, 885, 1186, 1580, 1724
Lsp1109I GCAGC 8 cut(s) 238, 757, 801, 1368, 1700, 1714, 1890, 2676
LweI GCATC 3 cut(s) 406, 428, 669
MaeI CTAG 7 cut(s) 123, 267, 897, 918, 1989, 2256, 2321
MaeII ACGT 4 cut(s) 407, 717, 1393, 2734
MaeIII GTNAC 5 cut(s) 86, 357, 403, 1317, 2290
MbiI CCGCTC 1 cut(s) 457
MfeI CAATTG 1 cut(s) 1451
MflI RGATCY 4 cut(s) 1171, 1402, 2500, 2672
MhlI GDGCHC 3 cut(s) 444, 1183, 1239
MlsI TGGCCA 2 cut(s) 348, 2048
MluNI TGGCCA 2 cut(s) 348, 2048
MlyI GAGTC 4 cut(s) 233, 830, 2022, 2275
MmeI TCCRAC 3 cut(s) 738, 2440, 2593
Mox20I TGGCCA 2 cut(s) 348, 2048
Mph1103I ATGCAT 1 cut(s) 421
MscI TGGCCA 2 cut(s) 348, 2048
MseI TTAA 5 cut(s) 701, 804, 1349, 1463, 1953
MslI CAYNNNNRTG 3 cut(s) 471, 2187, 2495
Msp20I TGGCCA 2 cut(s) 348, 2048
MspA1I CMGCKG 1 cut(s) 1381
MspI CCGG 3 cut(s) 362, 498, 606
MspR9I CCNGG 7 cut(s) 74, 413, 499, 595, 1400, 2050, 2313
MunI CAATTG 1 cut(s) 1451
Mva1269I GAATGC 4 cut(s) 11, 967, 1060, 2692
MvaI CCWGG 6 cut(s) 74, 413, 595, 1400, 2050, 2313
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 2 cut(s) 1697, 2313
NciI CCSGG 1 cut(s) 499
NlaIII CATG 9 cut(s) 71, 470, 476, 1179, 1492, 1868, 2047, 2568, 2602
NlaIV GGNNCC 4 cut(s) 496, 504, 2308, 2502
NmeAIII GCCGAG 1 cut(s) 1815
NmuCI GTSAC 1 cut(s) 403
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 3 cut(s) 476, 1492, 2568
NspV TTCGAA 1 cut(s) 369
OliI CACNNNNGTG 1 cut(s) 2495
PaeR7I CTCGAG 1 cut(s) 1267
PciI ACATGT 1 cut(s) 472
PctI GAATGC 4 cut(s) 11, 967, 1060, 2692
PfeI GAWTC 5 cut(s) 188, 794, 1831, 2349, 2517
Pfl23II CGTACG 1 cut(s) 234
PfoI TCCNGGA 2 cut(s) 411, 1398
PinAI ACCGGT 1 cut(s) 605
PkrI GCNGC 8 cut(s) 228, 772, 816, 1383, 1690, 1729, 1905, 2666
Ple19I CGATCG 1 cut(s) 658
PleI GAGTC 4 cut(s) 233, 829, 2022, 2275
PpsI GAGTC 4 cut(s) 233, 829, 2022, 2275
Ppu21I YACGTR 1 cut(s) 1394
PscI ACATGT 1 cut(s) 472
PshAI GACNNNNGTC 1 cut(s) 606
PshBI ATTAAT 1 cut(s) 804
Psp124BI GAGCTC 1 cut(s) 444
Psp6I CCWGG 6 cut(s) 72, 411, 593, 1398, 2048, 2311
PspGI CCWGG 6 cut(s) 72, 411, 593, 1398, 2048, 2311
PspLI CGTACG 1 cut(s) 234
PspN4I GGNNCC 4 cut(s) 496, 504, 2308, 2502
PspPI GGNCC 1 cut(s) 383
PspXI VCTCGAGB 1 cut(s) 1267
PstI CTGCAG 1 cut(s) 2272
PstNI CAGNNNCTG 2 cut(s) 2132, 2632
PsuI RGATCY 4 cut(s) 1171, 1402, 2500, 2672
PvuI CGATCG 1 cut(s) 658
PvuII CAGCTG 1 cut(s) 1381
RsaI GTAC 9 cut(s) 36, 236, 471, 625, 845, 1193, 2042, 2333, 2388
RsaNI GTAC 9 cut(s) 35, 235, 470, 624, 844, 1192, 2041, 2332, 2387
RseI CAYNNNNRTG 3 cut(s) 471, 2187, 2495
SacI GAGCTC 1 cut(s) 444
SalI GTCGAC 1 cut(s) 719
SaqAI TTAA 5 cut(s) 701, 804, 1349, 1463, 1953
SatI GCNGC 8 cut(s) 227, 771, 815, 1382, 1689, 1728, 1904, 2665
Sau96I GGNCC 1 cut(s) 383
SbfI CCTGCAGG 1 cut(s) 2272
ScaI AGTACT 2 cut(s) 36, 1193
SchI GAGTC 4 cut(s) 233, 830, 2022, 2275
ScrFI CCNGG 7 cut(s) 74, 413, 499, 595, 1400, 2050, 2313
SdaI CCTGCAGG 1 cut(s) 2272
SduI GDGCHC 3 cut(s) 444, 1183, 1239
SfaNI GCATC 3 cut(s) 406, 428, 669
SfcI CTRYAG 3 cut(s) 277, 2268, 2371
Sfr274I CTCGAG 1 cut(s) 1267
SfuI TTCGAA 1 cut(s) 369
SinI GGWCC 1 cut(s) 383
SlaI CTCGAG 1 cut(s) 1267
SmiMI CAYNNNNRTG 3 cut(s) 471, 2187, 2495
SmlI CTYRAG 5 cut(s) 1240, 1267, 1766, 1805, 1887
SmoI CTYRAG 5 cut(s) 1240, 1267, 1766, 1805, 1887
Sse8387I CCTGCAGG 1 cut(s) 2272
SsiI CCGC 5 cut(s) 7, 455, 705, 1536, 1747
SspMI CTAG 7 cut(s) 123, 267, 897, 918, 1989, 2256, 2321
SstI GAGCTC 1 cut(s) 444
StyD4I CCNGG 7 cut(s) 72, 411, 497, 593, 1398, 2048, 2311
StyI CCWWGG 2 cut(s) 579, 2504
TaaI ACNGT 5 cut(s) 234, 699, 1785, 1858, 2646
TaiI ACGT 4 cut(s) 410, 720, 1396, 2737
TatI WGTACW 7 cut(s) 34, 469, 843, 1191, 2040, 2331, 2386
TfiI GAWTC 5 cut(s) 188, 794, 1831, 2349, 2517
Tru1I TTAA 5 cut(s) 701, 804, 1349, 1463, 1953
Tru9I TTAA 5 cut(s) 701, 804, 1349, 1463, 1953
TscAI CASTG 3 cut(s) 408, 772, 2633
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 8 cut(s) 226, 770, 814, 1381, 1688, 1727, 1903, 2664
Tsp45I GTSAC 1 cut(s) 403
TspGWI ACGGA 1 cut(s) 1284
TspRI CASTG 3 cut(s) 408, 772, 2633
VpaK11BI GGWCC 1 cut(s) 383
VspI ATTAAT 1 cut(s) 804
XagI CCTNNNNNAGG 1 cut(s) 1705
XapI RAATTY 4 cut(s) 1330, 1387, 2221, 2260
XbaI TCTAGA 2 cut(s) 896, 2255
XceI RCATGY 3 cut(s) 476, 1492, 2568
XcmI CCANNNNNNNNNTGG 1 cut(s) 2666
XhoI CTCGAG 1 cut(s) 1267
XmiI GTMKAC 2 cut(s) 720, 2110
XspI CTAG 7 cut(s) 123, 267, 897, 918, 1989, 2256, 2321
ZrmI AGTACT 2 cut(s) 36, 1193
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.