FvH4_6g32690

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000083
Physical Location & Seq
Reverse (-)
25761225 .. 25765680
4456 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32690.t2

Sequence Viewer

Length: 2361 bp
ATGTGGGGGACATTGATGAATTCGTTGGTCTTCGCAGTTTTAGTACTGCTTGGTGGTTTTCCTCTCAACCTGAAGCTCCTTGTTCATGCCCAGGATCAATCAGGCTTCATTAGCATAGATTGTGGACTGCCGGCAGAAAATTTAAGCTATATTGAAAGGACAACCGGCATCAACTATACTTCAGATGCCAACTTCATTGACACTGGTGAAATTAAGTTGGTACATCCTGAATACAGAGATACAAAGCAGCAGCCATACGAGTCTCTAAGGTTGTTTCCTGAAGGGACAAGGAACTGCTACAAAATAAACGTTACAAGTTGCACCAAGTATTTGATCCGAGCATGTTTCTTTTATGGGAACTATGATGGTCAAGATAAATCACCTGAATTTGAACTACATTTTGGTGCTAATTTGTGGGATTCAGTGAGTTTTGATTATGCATCCACAGACATAAACAAGGAGCTTATACATTTTGTACCTGATGGAGTCTCAGATGTACAAGTTTGTCTGGTGAACACTGGCTCCGGGGTTCCATTTATATCAGGGATAGAGCTGAGGCCTTTAAATAATGAATCTTATGAAGCAGAAGTGGGATCGTTGGCGCTTGAATGGCGATATGACACAGGTCTAATTGCTAATAAGAGAGGATACAGGTATCCAGAGGATATTCATGATCGCTTCTGGTATTTCTACGAAAGCCTTGACGATTGGGAACAATTAAATACTTCATCCACAATCGACCCTCTCAATCAGAACATAGTCGAATTACCATCTATTGTTATGAGTACTGCTGTCAAGCCAAAAAATGTAAATGAGTCCTTGAATTTTTCTTGGCAGCCTACTGAGCAAAATGCAGAATATCATATATTCATGCATTTCGCAGAAGTTGAAAAGCTCCAACCAAACCAATCTAGATGGCTATACATTACTAAAAATGGGAAGATGTATAATGACCCATTTGAATTGGGGTACATGTACCCATCTACTAGTTACAGAAGTAAAGCAATGAGTGGAGGACAAAACTTTTCAATCTTCAAGGTCGAGAACTCCACCCTTCCACCCATCCTCAATGCTTTTGAGCTTTATAAGGTGAAAAAATTCATAGAACCGGTTACAAGTCAAGGAGATGAAGAAACTGGCAAGGAGATCCTTGTGCCCCCCAAGTTTACTCATGGCAAGAACTTGGAGAACAACAATCTCAAAGGCTTGGTTCCAGTTGGACTGATTGAAAGAATGAATGATGGTTCACTAACATTAAGTTTGTGCCAAAATCCAAATGTATCTGGACTTGTTTCTTGCACGAAGATGGAAAAGAAGAAGCCAAAGTTCTTAACTCCGGTAGTTCTGTCAATTGCTGGAGTGTCAATCTTCTTATTAACTATAACAGCTATCTTGTGGGGTTTAAGAAGGAAAAAGAAACATGATGCAAAATCCAAAACTGAGTTAGGATCATTGGAGTCAACTAAACGACAATTTACGCATTCTGAGATCCTGAAATTCACCAACAACTTGAAAAGGACTCTTGGGAAAGGTGGATTTGGAACAGTTTATCATGGTTACATAGACAAAACTCAAGTTAATCTTCTGATGAGAGTTCATCACACAAACTTGACCAGCCTTGTTGGATATTGCAATGAGGAATCCAATATAGGGCTCGTCTACGAGTATATGGCCAATGGAAACTTACAAGAACATCTTTCAGATGACAGTTCCACTATTTTGGGTTGGGAAGATAGACTTCGGATAGCAGTAGATGCTGCACAAGGATTGGAGTATCTGCATAATGGTTGTAAGCCACCTATAATTCACAGGGATGTGAAATCAGCAAACATCTTGCTGAGTGAAAATTTCCAAGCCAAAGTAGCTGATTTTGGCCTATCCAGAATTTTCCATACAGACCTTGTAACTCATATAACAACTGGTATCGCTGGTACTCCCAGGTACTACCTAACAAGCAGGTTAAATGAGAAAAGTGATGTTTATAGCTTTGGGATTGTGCTGTTGGAAATTATCACAAGCCGACCTGTTATATGGGGAACTGTTGAGAGAATTCACATTAGTCACTGGGTTGCTTCCATGCTTGCACAAGGAGACATTTACAGTGTTGTTGAGCCAAGACTGGAGAGAAATTTCAGTCCTAACTGTGTGTGGCAAGTTTTGGAGATAGCAATGGCATGTATATCTCCAGACCCCATCAAAAGGCCAATGATGAGTCAGGTAGTGATGGAACTGAAGGAGTGTTTGGCAATCGAACTGGCTCGAACAAAGCAGACAAGAAATGAAACTGATCTAGGACATTCCACTGAGAGATTGTCTCAGAACGAGTCGATCAGAGTACATCCCTCAGTTATAAGGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

787

Amino Acids

89.04

Weight (kDa)

6.09

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 39 - 364 1.3e-102 Malectin-like domain
PK_Tyr_Ser-Thr PF07714 504 - 764 4.9e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 504 - 761 5.1e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000083)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07560 AT1G51790 AT1G51790 AT1G51790 AT1G51800 AT1G51805 AT1G51805 AT1G51810 AT1G51820 AT1G51820 AT1G51830 AT1G51830 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51840 AT1G51850 AT1G51850 AT1G51860 AT1G51860 AT1G51870 AT1G51870 AT1G51880 AT1G51880 AT1G51880 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51890 AT1G51910 AT2G04300 AT2G04300 AT2G28960 AT2G28960 AT2G28970 AT2G28970 AT2G28990 AT2G28990 AT2G29000 AT3G21340 AT3G46350 AT5G59616 AT5G59650 AT5G59650 AT5G59660 AT5G59660 AT5G59660 AT5G59670 AT5G59670 AT5G59680
fragaria_vesca FvH4_2g03270 FvH4_3g21300 FvH4_6g32660 FvH4_6g32660 FvH4_6g32670 FvH4_6g32670 FvH4_6g32690 FvH4_6g32690 FvH4_6g32691 FvH4_6g32700 FvH4_6g32740 FvH4_6g32750 FvH4_6g33210 FvH4_6g33210 FvH4_7g24270 FvH4_7g24280 FvH4_7g24291 FvH4_7g24300
malus_domestica MD09G1198400.v1.1 MD09G1198600.v1.1 MD09G1199100.v1.1 MD09G1199200.v1.1 MD09G1199300.v1.1 MD09G1199500.v1.1 MD09G1199600.v1.1 MD17G1179300.v1.1 MD17G1180200.v1.1 MD17G1266000.v1.1
prunus_persica Prupe.1G438900_v2.0.a1 Prupe.1G439000_v2.0.a1 Prupe.1G439100_v2.0.a1 Prupe.3G019400_v2.0.a1 Prupe.3G021900_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G054500_v2.0.a1 Prupe.3G055100_v2.0.a1 Prupe.3G055400_v2.0.a1 Prupe.3G055500_v2.0.a1 Prupe.3G055600_v2.0.a1 Prupe.3G055700_v2.0.a1 Prupe.3G055800_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G055900_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056000_v2.0.a1 Prupe.3G056100_v2.0.a1 Prupe.3G209600_v2.0.a1 Prupe.3G209700_v2.0.a1 Prupe.6G291100_v2.0.a1 Prupe.7G208900_v2.0.a1 Prupe.8G038300_v2.0.a1
pyrus_communis pycom09g11570 pycom09g11580 pycom09g11610 pycom09g11630 pycom09g11640 pycom17g18840 pycom17g18910 pycom17g18930 pycom17g18940
rosa_chinensis RchiOBHm_Chr1g0317521 RchiOBHm_Chr1g0317541 RchiOBHm_Chr2g0123351 RchiOBHm_Chr2g0141181 RchiOBHm_Chr2g0141191 RchiOBHm_Chr2g0141211 RchiOBHm_Chr2g0141221 RchiOBHm_Chr2g0141261 RchiOBHm_Chr2g0141271 RchiOBHm_Chr2g0141311 RchiOBHm_Chr2g0141331 RchiOBHm_Chr2g0141371 RchiOBHm_Chr2g0141391 RchiOBHm_Chr2g0141401 RchiOBHm_Chr2g0141431 RchiOBHm_Chr2g0141481 RchiOBHm_Chr2g0141491 RchiOBHm_Chr2g0141591 RchiOBHm_Chr2g0141601 RchiOBHm_Chr2g0141611 RchiOBHm_Chr2g0141631 RchiOBHm_Chr2g0141641 RchiOBHm_Chr2g0141661 RchiOBHm_Chr2g0141681 RchiOBHm_Chr2g0141701 RchiOBHm_Chr2g0141781 RchiOBHm_Chr2g0141811 RchiOBHm_Chr2g0141821 RchiOBHm_Chr3g0479311 RchiOBHm_Chr3g0495751 RchiOBHm_Chr4g0404741 RchiOBHm_Chr4g0410341 RchiOBHm_Chr4g0418371 RchiOBHm_Chr4g0438651 RchiOBHm_Chr5g0036411 RchiOBHm_Chr5g0036491 RchiOBHm_Chr5g0042391 RchiOBHm_Chr6g0279871 RchiOBHm_Chr7g0192921 RchiOBHm_Chr7g0214901 RchiOBHm_Chr7g0234931
rosa_laevigata RLG00000003432 RLG00000008806 RLG00000013062 RLG00000019514 RLG00000019923 RLG00000019924 RLG00000019925 RLG00000019930 RLG00000019931 RLG00000019934 RLG00000019937 RLG00000019938 RLG00000033697
rosa_multiflora Rmu_co8002302.1_g000001 Rmu_sc0000147.1_g000031 Rmu_sc0000463.1_g000005 Rmu_sc0000712.1_g000003 Rmu_sc0001483.1_g000017 Rmu_sc0001554.1_g000002 Rmu_sc0001554.1_g000005 Rmu_sc0001554.1_g000008 Rmu_sc0001981.1_g000017 Rmu_sc0002040.1_g000056 Rmu_sc0002755.1_g000018 Rmu_sc0002804.1_g000016 Rmu_sc0002928.1_g000015 Rmu_sc0003040.1_g000008 Rmu_sc0003040.1_g000018 Rmu_sc0004512.1_g000001 Rmu_sc0004512.1_g000004 Rmu_sc0005507.1_g000001 Rmu_sc0005507.1_g000009 Rmu_sc0005507.1_g000033 Rmu_sc0005507.1_g000037 Rmu_sc0005507.1_g000043 Rmu_sc0005507.1_g000047 Rmu_sc0006318.1_g000004 Rmu_sc0006318.1_g000014 Rmu_sc0006318.1_g000021 Rmu_sc0006556.1_g000001 Rmu_sc0007208.1_g000002 Rmu_sc0007208.1_g000003 Rmu_sc0007208.1_g000006 Rmu_sc0007208.1_g000008
rosa_roxburghii Rroxscaffold_1G00044430 Rroxscaffold_2G00103420 Rroxscaffold_2G00103470 Rroxscaffold_2G00103490 Rroxscaffold_2G00103500 Rroxscaffold_2G00103520 Rroxscaffold_2G00103530 Rroxscaffold_2G00103540 Rroxscaffold_2G00103550 Rroxscaffold_2G00103570 Rroxscaffold_2G00103770 Rroxscaffold_2G00103780 Rroxscaffold_2G00103800 Rroxscaffold_2G00103820 Rroxscaffold_2G00103860 Rroxscaffold_2G00103890 Rroxscaffold_2G00103930 Rroxscaffold_2G00103940 Rroxscaffold_2G00104010 Rroxscaffold_2G00104030 Rroxscaffold_2G00104050 Rroxscaffold_2G00104060 Rroxscaffold_2G00104070 Rroxscaffold_2G00104090 Rroxscaffold_2G00104100 Rroxscaffold_2G00104120 Rroxscaffold_2G00104130 Rroxscaffold_4G00331710
rosa_rugosa Rorug02G0367500 Rorug02G0367500 Rorug02G0367600 Rorug02G0367800 Rorug02G0367800 Rorug02G0367900 Rorug02G0368100 Rorug02G0368100 Rorug02G0368100 Rorug02G0368200 Rorug02G0368700 Rorug02G0368900 Rorug05G0155600 Rorug05G0155700 Rorug05G0155800 Rorug05G0156700 Rorug05G0157300
rosa_samantha Rh1AG028000 Rh2AG418700 Rh2AG418900 Rh2AG419000 Rh2AG419100 Rh2AG419400 Rh2AG419600 Rh2AG419800 Rh2AG420400 Rh2AG421000 Rh2BG428500 Rh2BG428600 Rh2BG428800 Rh2BG428900 Rh2BG429300 Rh2BG429400 Rh2BG429700 Rh2BG429900 Rh2BG430000 Rh2BG430700 Rh2CG405100 Rh2CG405200 Rh2CG405300 Rh2CG405500 Rh2CG405600 Rh2CG405700 Rh2CG406000 Rh2CG406100 Rh2CG406300 Rh2CG406600 Rh2CG406700 Rh2CG407500 Rh2CG408100 Rh2DG438500 Rh3DG123900 Rh3DG124000 Rh5BG249700 Rh5BG482500 Rh5CG281500 Rh5CG507300 Rh7DG133300
rosa_wichuraiana Rw0G019720 Rw0G019730 Rw0G019750 Rw0G023010 Rw2G034340 Rw2G034350 Rw2G034370 Rw2G034380 Rw2G034400 Rw2G034430 Rw2G034470 Rw2G034480 Rw2G034510 Rw5G022890 Rw5G026410 Rw5G032710 Rw5G042850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1086, 2351
Acc36I ACCTGC 1 cut(s) 1947
AccI GTMKAC 1 cut(s) 1659
AclI AACGTT 1 cut(s) 309
AclWI GGATC 6 cut(s) 102, 328, 601, 1141, 1456, 1483
AcoI YGGCCR 1 cut(s) 1671
AcuI CTGAAG 4 cut(s) 92, 165, 300, 2252
AfiI CCNNNNNNNGG 2 cut(s) 1650, 2199
AflIII ACRYGT 1 cut(s) 972
AgeI ACCGGT 1 cut(s) 1108
AhlI ACTAGT 1 cut(s) 986
AjnI CCWGG 2 cut(s) 90, 1937
AjuI GAANNNNNNNTTGG 4 cut(s) 384, 416, 2225, 2257
AloI GAACNNNNNNTCC 2 cut(s) 506, 538
AluBI AGCT 9 cut(s) 76, 147, 463, 553, 895, 1081, 1388, 1865, 1986
AluI AGCT 9 cut(s) 76, 147, 463, 553, 895, 1081, 1388, 1865, 1986
Alw26I GTCTC 4 cut(s) 267, 493, 2085, 2319
AlwI GGATC 6 cut(s) 102, 328, 601, 1141, 1456, 1483
AoxI GGCC 4 cut(s) 557, 1671, 1873, 2201
ApeKI GCWGC 4 cut(s) 247, 250, 835, 1757
AsiGI ACCGGT 1 cut(s) 1108
Asp700I GAANNNNTTC 1 cut(s) 1097
AspLEI GCGC 1 cut(s) 604
AsuC2I CCSGG 1 cut(s) 526
AsuHPI GGTGA 5 cut(s) 218, 372, 523, 1102, 1492
BaeGI GKGCMC 1 cut(s) 1158
BalI TGGCCA 1 cut(s) 1673
BanII GRGCYC 1 cut(s) 1656
BbsI GAAGAC 1 cut(s) 22
BbvCI CCTCAGC 1 cut(s) 554
BbvI GCAGC 4 cut(s) 259, 262, 847, 1744
BciT130I CCWGG 2 cut(s) 92, 1939
BciVI GTATCC 2 cut(s) 641, 666
BcnI CCSGG 1 cut(s) 526
BcoDI GTCTC 4 cut(s) 267, 493, 2085, 2319
BcuI ACTAGT 1 cut(s) 986
BfaI CTAG 3 cut(s) 912, 987, 2291
BfoI RGCGCY 1 cut(s) 605
BfuAI ACCTGC 1 cut(s) 1947
BfuI GTATCC 2 cut(s) 641, 666
BisI GCNGC 4 cut(s) 248, 251, 836, 1758
BlsI GCNGC 4 cut(s) 249, 252, 837, 1759
BmcAI AGTACT 2 cut(s) 45, 787
Bme1390I CCNGG 3 cut(s) 92, 526, 1939
BmiI GGNNCC 3 cut(s) 523, 531, 1212
BmrFI CCNGG 3 cut(s) 92, 526, 1939
BmrI ACTGGG 1 cut(s) 2074
BmsI GCATC 5 cut(s) 175, 177, 449, 1414, 1744
BmuI ACTGGG 1 cut(s) 2074
BoxI GACNNNNGTC 1 cut(s) 624
BpiI GAAGAC 1 cut(s) 22
BplI GAGNNNNNCTC 2 cut(s) 2299, 2331
BpmI CTGGAG 3 cut(s) 1377, 2141, 2169
Bpu10I CCTNAGC 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 1557
BpuMI CCSGG 1 cut(s) 526
BsaJI CCNNGG 3 cut(s) 90, 525, 1937
BsaWI WCCGGW 2 cut(s) 1108, 1336
BsaXI ACNNNNNCTCC 2 cut(s) 506, 536
Bsc4I CCNNNNNNNGG 2 cut(s) 1650, 2199
Bse118I RCCGGY 3 cut(s) 130, 164, 1108
Bse1I ACTGG 8 cut(s) 208, 523, 1141, 1214, 1924, 2069, 2124, 2259
Bse3DI GCAATG 3 cut(s) 1011, 1639, 2175
BseBI CCWGG 2 cut(s) 92, 1939
BseDI CCNNGG 3 cut(s) 90, 525, 1937
BseGI GGATG 6 cut(s) 223, 440, 728, 1062, 1819, 2338
BseLI CCNNNNNNNGG 2 cut(s) 1650, 2199
BseMI GCAATG 3 cut(s) 1011, 1639, 2175
BseMII CTCAG 9 cut(s) 504, 545, 834, 1431, 1476, 1829, 2295, 2330, 2358
BseNI ACTGG 8 cut(s) 208, 523, 1141, 1214, 1924, 2069, 2124, 2259
BseSI GKGCMC 1 cut(s) 1158
BseXI GCAGC 4 cut(s) 259, 262, 847, 1744
BsgI GTGCAG 1 cut(s) 1743
BshFI GGCC 4 cut(s) 559, 1673, 1875, 2203
BshTI ACCGGT 1 cut(s) 1108
BsiSI CCGG 5 cut(s) 131, 165, 525, 1109, 1337
BslFI GGGAC 2 cut(s) 22, 298
BslI CCNNNNNNNGG 2 cut(s) 1650, 2199
BsmAI GTCTC 4 cut(s) 267, 493, 2085, 2319
BsmFI GGGAC 2 cut(s) 22, 298
BsmI GAATGC 1 cut(s) 1480
BsnI GGCC 4 cut(s) 559, 1673, 1875, 2203
Bsp1286I GDGCHC 2 cut(s) 1158, 1656
Bsp1407I TGTACA 1 cut(s) 496
Bsp143I GATC 9 cut(s) 94, 333, 593, 673, 1146, 1448, 1488, 2287, 2328
BspANI GGCC 4 cut(s) 559, 1673, 1875, 2203
BspCNI CTCAG 9 cut(s) 503, 546, 835, 1432, 1477, 1830, 2296, 2329, 2357
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 3 cut(s) 523, 531, 1212
BspMI ACCTGC 1 cut(s) 1947
BspPI GGATC 6 cut(s) 102, 328, 601, 1141, 1456, 1483
BsrDI GCAATG 3 cut(s) 1011, 1639, 2175
BsrFI RCCGGY 3 cut(s) 130, 164, 1108
BsrGI TGTACA 1 cut(s) 496
BsrI ACTGG 8 cut(s) 208, 523, 1141, 1214, 1924, 2069, 2124, 2259
BssAI RCCGGY 3 cut(s) 130, 164, 1108
BssECI CCNNGG 3 cut(s) 90, 525, 1937
BssMI GATC 9 cut(s) 94, 333, 593, 673, 1146, 1448, 1488, 2287, 2328
Bst2UI CCWGG 2 cut(s) 92, 1939
Bst4CI ACNGT 5 cut(s) 1546, 1709, 2041, 2102, 2144
BstAPI GCANNNNNTGC 1 cut(s) 1754
BstAUI TGTACA 1 cut(s) 496
BstC8I GCNNGC 2 cut(s) 132, 2082
BstF5I GGATG 6 cut(s) 223, 440, 728, 1062, 1819, 2338
BstH2I RGCGCY 1 cut(s) 605
BstHHI GCGC 1 cut(s) 604
BstKTI GATC 9 cut(s) 97, 336, 596, 676, 1149, 1451, 1491, 2290, 2331
BstMAI GTCTC 4 cut(s) 267, 493, 2085, 2319
BstMBI GATC 9 cut(s) 94, 333, 593, 673, 1146, 1448, 1488, 2287, 2328
BstMWI GCNNNNNNNGC 5 cut(s) 111, 610, 844, 1754, 1862
BstNI CCWGG 2 cut(s) 92, 1939
BstNSI RCATGY 3 cut(s) 345, 976, 2178
BstPAI GACNNNNGTC 1 cut(s) 624
BstSCI CCNGG 3 cut(s) 90, 524, 1937
BstSLI GKGCMC 1 cut(s) 1158
BstV1I GCAGC 4 cut(s) 259, 262, 847, 1744
BstV2I GAAGAC 1 cut(s) 22
BstX2I RGATCY 2 cut(s) 1146, 1488
BstXI CCANNNNNNTGG 1 cut(s) 1720
BstYI RGATCY 2 cut(s) 1146, 1488
BsuI GTATCC 2 cut(s) 641, 666
BsuRI GGCC 4 cut(s) 559, 1673, 1875, 2203
BtsCI GGATG 6 cut(s) 223, 440, 728, 1062, 1819, 2338
BtsIMutI CAGTG 6 cut(s) 201, 429, 516, 2062, 2107, 2301
BveI ACCTGC 1 cut(s) 1947
Cac8I GCNNGC 2 cut(s) 132, 2082
CciI TCATGA 1 cut(s) 670
CfoI GCGC 1 cut(s) 604
Cfr10I RCCGGY 3 cut(s) 130, 164, 1108
CspAI ACCGGT 1 cut(s) 1108
DpnI GATC 9 cut(s) 96, 335, 595, 675, 1148, 1450, 1490, 2289, 2330
DpnII GATC 9 cut(s) 94, 333, 593, 673, 1146, 1448, 1488, 2287, 2328
DraI TTTAAA 1 cut(s) 564
EaeI YGGCCR 1 cut(s) 1671
Eco147I AGGCCT 1 cut(s) 559
Eco24I GRGCYC 1 cut(s) 1656
Eco57I CTGAAG 4 cut(s) 92, 165, 300, 2252
EcoRI GAATTC 2 cut(s) 19, 2049
EcoRII CCWGG 2 cut(s) 90, 1937
EcoT22I ATGCAT 2 cut(s) 442, 876
EcoT38I GRGCYC 1 cut(s) 1656
FalI AAGNNNNNCTT 6 cut(s) 1566, 1598, 1680, 1712, 1722, 1754
FaqI GGGAC 2 cut(s) 22, 298
FblI GTMKAC 1 cut(s) 1659
Fnu4HI GCNGC 4 cut(s) 248, 251, 836, 1758
FokI GGATG 6 cut(s) 210, 427, 715, 1049, 1826, 2325
FriOI GRGCYC 1 cut(s) 1656
Fsp4HI GCNGC 4 cut(s) 248, 251, 836, 1758
FspBI CTAG 3 cut(s) 912, 987, 2291
GlaI GCGC 1 cut(s) 603
GluI GCNGC 4 cut(s) 248, 251, 836, 1758
GsuI CTGGAG 3 cut(s) 1377, 2141, 2169
HaeII RGCGCY 1 cut(s) 605
HaeIII GGCC 4 cut(s) 559, 1673, 1875, 2203
HapII CCGG 5 cut(s) 131, 165, 525, 1109, 1337
HhaI GCGC 1 cut(s) 604
Hin6I GCGC 1 cut(s) 602
HinP1I GCGC 1 cut(s) 602
HincII GTYRAC 1 cut(s) 1461
HindII GTYRAC 1 cut(s) 1461
HpaII CCGG 5 cut(s) 131, 165, 525, 1109, 1337
HphI GGTGA 5 cut(s) 218, 372, 523, 1102, 1492
Hpy166II GTNNAC 6 cut(s) 125, 514, 1167, 1247, 1461, 1660
Hpy8I GTNNAC 6 cut(s) 125, 514, 1167, 1247, 1461, 1660
HpyAV CCTTC 4 cut(s) 275, 1064, 1401, 2227
HpyCH4III ACNGT 5 cut(s) 1546, 1709, 2041, 2102, 2144
HpyCH4IV ACGT 1 cut(s) 309
HpyF10VI GCNNNNNNNGC 5 cut(s) 111, 610, 844, 1754, 1862
HpySE526I ACGT 1 cut(s) 309
HspAI GCGC 1 cut(s) 602
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Kzo9I GATC 9 cut(s) 94, 333, 593, 673, 1146, 1448, 1488, 2287, 2328
LmnI GCTCC 4 cut(s) 81, 460, 527, 900
Lsp1109I GCAGC 4 cut(s) 259, 262, 847, 1744
LweI GCATC 5 cut(s) 175, 177, 449, 1414, 1744
MaeI CTAG 3 cut(s) 912, 987, 2291
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 6 cut(s) 310, 989, 1111, 1556, 1903, 2060
MalI GATC 9 cut(s) 96, 335, 595, 675, 1148, 1450, 1490, 2289, 2330
MboI GATC 9 cut(s) 94, 333, 593, 673, 1146, 1448, 1488, 2287, 2328
MboII GAAGA 9 cut(s) 22, 952, 1024, 1142, 1315, 1327, 1360, 1574, 1742
MfeI CAATTG 1 cut(s) 1350
MflI RGATCY 2 cut(s) 1146, 1488
MhlI GDGCHC 2 cut(s) 1158, 1656
MlsI TGGCCA 1 cut(s) 1673
MluNI TGGCCA 1 cut(s) 1673
MlyI GAGTC 7 cut(s) 269, 495, 824, 1466, 1513, 2221, 2333
MmeI TCCRAC 4 cut(s) 922, 1198, 1603, 1983
MnlI CCTC 9 cut(s) 72, 549, 638, 655, 753, 1007, 1076, 1630, 2353
Mox20I TGGCCA 1 cut(s) 1673
Mph1103I ATGCAT 2 cut(s) 442, 876
MroNI GCCGGC 1 cut(s) 130
MroXI GAANNNNTTC 1 cut(s) 1097
MscI TGGCCA 1 cut(s) 1673
MslI CAYNNNNRTG 3 cut(s) 402, 1304, 1812
Msp20I TGGCCA 1 cut(s) 1673
MspI CCGG 5 cut(s) 131, 165, 525, 1109, 1337
MspR9I CCNGG 3 cut(s) 92, 526, 1939
MunI CAATTG 1 cut(s) 1350
Mva1269I GAATGC 1 cut(s) 1480
MvaI CCWGG 2 cut(s) 92, 1939
MwoI GCNNNNNNNGC 5 cut(s) 111, 610, 844, 1754, 1862
NaeI GCCGGC 1 cut(s) 132
NciI CCSGG 1 cut(s) 526
NdeII GATC 9 cut(s) 94, 333, 593, 673, 1146, 1448, 1488, 2287, 2328
NgoMIV GCCGGC 1 cut(s) 130
NlaIV GGNNCC 3 cut(s) 523, 531, 1212
NmuCI GTSAC 1 cut(s) 2060
NsiI ATGCAT 2 cut(s) 442, 876
NspI RCATGY 3 cut(s) 345, 976, 2178
PagI TCATGA 1 cut(s) 670
PceI AGGCCT 1 cut(s) 559
PciI ACATGT 1 cut(s) 972
PctI GAATGC 1 cut(s) 1480
PdiI GCCGGC 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 1097
PfeI GAWTC 3 cut(s) 419, 572, 1640
PinAI ACCGGT 1 cut(s) 1108
PkrI GCNGC 4 cut(s) 249, 252, 837, 1759
PleI GAGTC 7 cut(s) 268, 494, 823, 1465, 1513, 2220, 2332
PpsI GAGTC 7 cut(s) 268, 494, 823, 1465, 1513, 2220, 2332
PscI ACATGT 1 cut(s) 972
PshAI GACNNNNGTC 1 cut(s) 624
PsiI TTATAA 2 cut(s) 1086, 2351
Psp1406I AACGTT 1 cut(s) 309
Psp6I CCWGG 2 cut(s) 90, 1937
PspGI CCWGG 2 cut(s) 90, 1937
PspN4I GGNNCC 3 cut(s) 523, 531, 1212
PsuI RGATCY 2 cut(s) 1146, 1488
RseI CAYNNNNRTG 3 cut(s) 402, 1304, 1812
SatI GCNGC 4 cut(s) 248, 251, 836, 1758
Sau3AI GATC 9 cut(s) 94, 333, 593, 673, 1146, 1448, 1488, 2287, 2328
ScaI AGTACT 2 cut(s) 45, 787
SchI GAGTC 7 cut(s) 269, 495, 824, 1466, 1513, 2221, 2333
ScrFI CCNGG 3 cut(s) 92, 526, 1939
SduI GDGCHC 2 cut(s) 1158, 1656
SfaNI GCATC 5 cut(s) 175, 177, 449, 1414, 1744
SmiMI CAYNNNNRTG 3 cut(s) 402, 1304, 1812
SmlI CTYRAG 1 cut(s) 1572
SmoI CTYRAG 1 cut(s) 1572
SpeI ACTAGT 1 cut(s) 986
SseBI AGGCCT 1 cut(s) 559
SspMI CTAG 3 cut(s) 912, 987, 2291
StuI AGGCCT 1 cut(s) 559
StyD4I CCNGG 3 cut(s) 90, 524, 1937
TaaI ACNGT 5 cut(s) 1546, 1709, 2041, 2102, 2144
TaiI ACGT 1 cut(s) 312
TaqI TCGA 6 cut(s) 738, 762, 1041, 2250, 2260, 2327
TatI WGTACW 4 cut(s) 43, 496, 785, 2335
TfiI GAWTC 3 cut(s) 419, 572, 1640
TscAI CASTG 6 cut(s) 208, 429, 523, 2069, 2107, 2308
TseFI GTSAC 1 cut(s) 2060
TseI GCWGC 4 cut(s) 247, 250, 835, 1757
Tsp45I GTSAC 1 cut(s) 2060
TspRI CASTG 6 cut(s) 208, 429, 523, 2069, 2107, 2308
XbaI TCTAGA 1 cut(s) 911
XceI RCATGY 3 cut(s) 345, 976, 2178
XmiI GTMKAC 1 cut(s) 1659
XmnI GAANNNNTTC 1 cut(s) 1097
XspI CTAG 3 cut(s) 912, 987, 2291
ZrmI AGTACT 2 cut(s) 45, 787
Zsp2I ATGCAT 2 cut(s) 442, 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.