FvH4_2g15190

Plant mobile domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000179
Physical Location & Seq
Forward (+)
13334665 .. 13337724
3060 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15190.t1

Sequence Viewer

Length: 2634 bp
ATGCTGAAGTCTGCCCATCATGATCAAAATGCTCCTCAGACACCGTTCGTACTTCTCAGCGATCGTATGTTCAATGGTTCAGTGCAATGGAGTGGGTTTCGTGCCAACAGAGCCTTCTCCTACACGGAACGTTATTGGGAGTGGTTAGAAGACATCTTAAGCCGAAATGGAGACCTCATCCACAAAACTGGCCTCTATCGAGCGGTGTTTGCATCGCTCTTCAGCTATGACCGTGTTGCGCCTGTGGTGCGGGCCTTCTGCGAGTATTGGTGCCCCAGCACGAATACTCTTCACACATCATGTGGCGAAATGTCTATTTCTTTGAGGGACTTGACATATATTTGTGGCTTGCCCATCACTGGAAGGTTTTACGACGAAGTTGTTCCACCTATCGAAGAATTTTCTGCCAAGGGTAAAGGAGGCGAACCGGTCATTCCCAAGAGTTGCAAGTTCTTATTTCTCTCATACCATAAGCTTTGCAAGGATGATAAAGGACAGAAGGTGAAGATCGTATCGTGGATCCAGTATTGGTACAAAGGAAGCGTGAGATACAAGAGGCCACCTACCAAGAGTGTACGGAACAAGAAAGATGCGCCTCAAAACACATACAACCCATCTGGTGAAATCCACGTGGTTCAAGCCCGAACTGATGACGATTCTGCACCATTTGATGACTTGGGCGTAGATGACGAAGACATAGAGTCTACTTACTTGGCTGCATTTTATGCGTGCTGGTTGTGTCTATTCATCTTTCCCATGGACGATGCAGGCTTAATCCGACCTAGTGTGTTCAAAGTCGCCAGGTTATCAATTCTGTCTAGCGATACCTGTGTTGGCCAACATTTATCAAGGCTTGAACGAGATTTCCGCTTCCAAGGACCCAGGGAATTGCTCGGCCACATTACCTTTTCACTACGTGTATGCGTGGTTGGGGCATTTTCGGCCAAGCATTTTGGAGATGCACAAGCTCGTTCTTTATTCAACACGTGTAGTAATGTGGGAATGGACACTTTAGCCATGATCCATAAAGAGCGCAAAGTGATCGTTGATCGCGAGAACATTAGCCGCCCAGACATCGTCTCCCTCATTTGCCTTCGATCCGGACTTCTTACTCTTAGACAAGACAACCAACGAGTCGTTCAACCCTACAGTCCTCATCGTTTCAGCCGACAGTTTGGTTACGTTCAAGATGTTCCAGGAAGCCTTAAAAAAGACACTCGCACGGGCGCATTATCCTTGATGTACTGGCATTGGGAAAGCCTTACTAAACGCGAAACGGGAAGCACATTGACGTTGCCACCTGAGAATGCTCTAACAGAGTATATGGTGACTCGTGGTTATGTTGAATGGTGGTCTAAAGTATATTGCTCCACGCCCAAGAAAACTACAGCAGAAATACCTGTTGGCGGGTCACAGCACAAGCCTCTAAAAGTGAAAGAAGACAAGATTGTCACTCGCACGGCGCCGCCTCGTCAAAAAGTGGTTCCTCGTGAAGTTGAAGTTGACAACAACCCTCATGCATCTTCCATCAAGGCGTTGCCCCCAAGTAGTAGCCCTCCGAATGAGCCGCATCAAACTCTAGAAGGTCATGGGGACTCATCCGATCATCGTCTTGCCCAGAGTAACCTTAAGCTGAAGGGGATTGACACCCAAGCAAAAAGGTTTAGTGGAAATAGTAGCAGCTCCAGGGACATGAACTTCAAACGTCAAAGAATTGAACCTGACCTTGGCTCCATTTATTTCAATCCCAACGATGAATTTCTCCTCAATAATGATTTCCTTGGCGACGTTCCAGGTCCTTCGCAACAAACACCGAACTACGTCGAGACTTCATCATTTGGTGGAGGACATATTTTAGTGGGCCTTGATACCCCCTTTCTCTCCTGGGCGTCACAACCAATAAAGACCGGCTCTTTTATTGAGGATGATGCATGCATCATCAAACATAATGAATTGCAACAAGTTAATTGTGAAGATATGACTGCAAAGGTGAACCCTTCTAGGCTTTTAAACAATGTGGCATTCTCTGAAGTCACCCGTGGTGGAGTTGATGTCATGGACACCAACCAGGGTCTCCGAAAGATTCGTATGCAGGCCGCCACTCTAATTTGTGAAGAAATTGAAGACATCATAACGAAGCACTCGTCAAGGACCATTCTTTCTAAGAAAAAAGACCTTGACGAGATGGTGGCCGAACTTAAATGTTATGGGGTTGATCCTTCATCAGTAAAAGACAAGGTAGAGAGCTTGATGACTTGTGTTACTCAATACAACGTGGCAAGGCTTGCATGCACACATAAAGCTACTCCAGGCACATGTGATCAACGTTTGTCTCATACGGAGGCTGAAATTGCAAAGTCGATCGAACTCCGTCAAACCGACCTTGGGCAACGTCAACCTGCAATTGAGTCCTTGGAGAATTTGAAGAAGGAGCAACAGAAGTTGGAGCAGAAAAAAACTTCCCTAGACGCGAGTCTTCTACTTCATGACAAAAAACTTGCTGATTTAGAGAAGCAGAAAGTCCAAATCAAGGAGATTCCAGAGTTGACTACTGAGGAGCTTAAGGAAGCAAAGAGGTTGACAGATGATTTTGAAAAGCAGCGCAACAGCTTCAAAGGCCTAACTTGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMD

Protein Analysis

878

Amino Acids

99.19

Weight (kDa)

8.09

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 64 - 267 1.5e-11 Plant mobile domain
PMD PF10536 379 - 452 2.6e-08 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000179)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18561 FvH4_1g29681 FvH4_2g15190 FvH4_3g22800 FvH4_3g22801 FvH4_3g26700 FvH4_4g15162 FvH4_4g15163 FvH4_4g15164 FvH4_4g22273 FvH4_7g02320 FvH4_7g03670 FvH4_7g03671
malus_domestica MD07G1097100.v1.1 MD11G1208600.v1.1 MD15G1303300.v1.1
prunus_persica Prupe.2G070800_v2.0.a1
pyrus_communis pycom02g13430 pycom04g06950 pycom04g09420 pycom04g09610 pycom06g05740 pycom07g00010 pycom07g06720 pycom07g09960 pycom07g10670 pycom07g10680 pycom07g10920 pycom07g27810 pycom08g12110 pycom08g13640 pycom09g03570 pycom09g13470 pycom09g13510 pycom10g01180 pycom10g02180 pycom10g06840 pycom12424g00130 pycom12g03090 pycom12g09570 pycom12g09580 pycom12g09590 pycom1310g00020 pycom13g25030 pycom13g25310 pycom13g25700 pycom13g26160 pycom13g27790 pycom13g28780 pycom13g28790 pycom1683g00070 pycom16g19330 pycom16g21210 pycom16g21710 pycom16g23400 pycom16g24730 pycom17g17550 pycom17g27670 pycom420g00110 pycom420g00130 pycom420g00610 pycom520g00600 pycom675g00050
rosa_chinensis RchiOBHm_Chr1g0316731
rosa_laevigata RLG00000030467
rosa_multiflora Rmu_co8112106.1_g000001 Rmu_co8301911.1_g000001 Rmu_sc0000161.1_g000042 Rmu_sc0000161.1_g000043 Rmu_sc0000173.1_g000035 Rmu_sc0000173.1_g000036 Rmu_sc0000480.1_g000024 Rmu_sc0000670.1_g000020 Rmu_sc0000670.1_g000021 Rmu_sc0000962.1_g000043 Rmu_sc0000962.1_g000044 Rmu_sc0001025.1_g000004 Rmu_sc0001075.1_g000026 Rmu_sc0001075.1_g000027 Rmu_sc0001167.1_g000050 Rmu_sc0001227.1_g000043 Rmu_sc0001346.1_g000006 Rmu_sc0001438.1_g000034 Rmu_sc0001946.1_g000002 Rmu_sc0001981.1_g000005 Rmu_sc0002106.1_g000005 Rmu_sc0002187.1_g000004 Rmu_sc0002187.1_g000005 Rmu_sc0002187.1_g000018 Rmu_sc0002539.1_g000097 Rmu_sc0003008.1_g000050 Rmu_sc0003008.1_g000051 Rmu_sc0003069.1_g000033 Rmu_sc0003069.1_g000034 Rmu_sc0003412.1_g000016 Rmu_sc0003553.1_g000005 Rmu_sc0003553.1_g000006 Rmu_sc0003641.1_g000025 Rmu_sc0003652.1_g000006 Rmu_sc0003687.1_g000019 Rmu_sc0003687.1_g000020 Rmu_sc0004234.1_g000013 Rmu_sc0004249.1_g000005 Rmu_sc0005044.1_g000027 Rmu_sc0005060.1_g000012 Rmu_sc0005816.1_g000005 Rmu_sc0006175.1_g000006 Rmu_sc0006175.1_g000007 Rmu_sc0006695.1_g000046 Rmu_sc0006725.1_g000014 Rmu_sc0006746.1_g000015 Rmu_sc0006746.1_g000016 Rmu_sc0006912.1_g000008 Rmu_sc0007355.1_g000004 Rmu_sc0007705.1_g000001 Rmu_sc0007903.1_g000003 Rmu_sc0007953.1_g000001 Rmu_sc0008630.1_g000001 Rmu_sc0008923.1_g000018 Rmu_sc0008923.1_g000019 Rmu_sc0009186.1_g000003 Rmu_sc0010184.1_g000007 Rmu_sc0010243.1_g000001 Rmu_sc0011242.1_g000004 Rmu_sc0012849.1_g000001 Rmu_sc0012849.1_g000002 Rmu_sc0012883.1_g000001 Rmu_sc0013028.1_g000015 Rmu_sc0015042.1_g000003 Rmu_sc0016730.1_g000002 Rmu_sc0017702.1_g000002 Rmu_sc0017791.1_g000007 Rmu_sc0017874.1_g000005 Rmu_sc0025334.1_g000001 Rmu_sc0025334.1_g000002 Rmu_sc0028789.1_g000001 Rmu_sc0030538.1_g000001 Rmu_sc0033137.1_g000001 Rmu_ssc0000183.1_g000013 Rmu_ssc0000259.1_g000061 Rmu_ssc0000259.1_g000062 Rmu_ssc0000259.1_g000063
rosa_roxburghii Rroxscaffold_173G00435030 Rroxscaffold_173G00435040 Rroxscaffold_1G00033030 Rroxscaffold_2G00093360 Rroxscaffold_2G00113080 Rroxscaffold_2G00146180 Rroxscaffold_3G00227920 Rroxscaffold_3G00251750 Rroxscaffold_4G00328240 Rroxscaffold_5G00333920 Rroxscaffold_5G00338240 Rroxscaffold_5G00342700 Rroxscaffold_6G00412810 Rroxscaffold_6G00423130 Rroxscaffold_7G00175190
rosa_rugosa Rorug01G0029200 Rorug01G0029300 Rorug01G0029400 Rorug01G0029400
rosa_samantha Rh1AG040900 Rh1BG038100 Rh1CG042000 Rh1DG027900
rosa_wichuraiana Rw0G019970 Rw1G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1448
Acc36I ACCTGC 1 cut(s) 2407
AccB1I GGYRCC 2 cut(s) 270, 1462
AccBSI CCGCTC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 704
AccII CGCG 3 cut(s) 1053, 1272, 2471
AccIII TCCGGA 1 cut(s) 1100
AciI CCGC 8 cut(s) 203, 250, 868, 1066, 1407, 1466, 1568, 2097
AclI AACGTT 2 cut(s) 130, 2326
AclWI GGATC 5 cut(s) 514, 527, 1015, 1092, 2210
AcoI YGGCCR 4 cut(s) 835, 895, 942, 2190
AcsI RAATTY 3 cut(s) 398, 1757, 2419
AcuI CTGAAG 4 cut(s) 26, 205, 1655, 2049
AcvI CACGTG 2 cut(s) 631, 987
AcyI GRCGYC 2 cut(s) 1463, 1889
AdeI CACNNNGTG 1 cut(s) 917
AfaI GTAC 4 cut(s) 51, 533, 576, 1244
AfiI CCNNNNNNNGG 5 cut(s) 359, 1406, 1727, 2385, 2530
AflII CTTAAG 3 cut(s) 157, 1628, 2561
AflIII ACRYGT 4 cut(s) 916, 984, 986, 2315
AgeI ACCGGT 1 cut(s) 427
AhdI GACNNNNNGTC 1 cut(s) 700
AjnI CCWGG 8 cut(s) 800, 881, 1195, 1685, 1792, 1883, 2067, 2308
AjuI GAANNNNNNNTTGG 8 cut(s) 98, 130, 1122, 1154, 1386, 1418, 1710, 1742
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
AluBI AGCT 9 cut(s) 225, 475, 968, 1633, 1683, 2247, 2303, 2560, 2610
AluI AGCT 9 cut(s) 225, 475, 968, 1633, 1683, 2247, 2303, 2560, 2610
Alw26I GTCTC 5 cut(s) 165, 1084, 1820, 2078, 2337
AlwI GGATC 5 cut(s) 514, 527, 1015, 1092, 2210
Aor13HI TCCGGA 1 cut(s) 1100
ApeKI GCWGC 3 cut(s) 716, 1680, 2599
ApoI RAATTY 3 cut(s) 398, 1757, 2419
ArsI GACNNNNNNTTYG 2 cut(s) 2129, 2161
AsiGI ACCGGT 1 cut(s) 427
Asp700I GAANNNNTTC 1 cut(s) 381
AspLEI GCGC 6 cut(s) 241, 595, 1035, 1229, 1465, 2604
AspS9I GGNCC 5 cut(s) 252, 878, 1796, 1861, 2151
AsuHPI GGTGA 5 cut(s) 514, 632, 1339, 2002, 2026
AvaII GGWCC 3 cut(s) 878, 1796, 2151
BaeGI GKGCMC 1 cut(s) 275
BaeI ACNNNNGTAYC 2 cut(s) 816, 849
BalI TGGCCA 1 cut(s) 837
BamHI GGATCC 1 cut(s) 519
BanI GGYRCC 2 cut(s) 270, 1462
BauI CACGAG 2 cut(s) 1332, 1488
BbrPI CACGTG 2 cut(s) 631, 987
BbsI GAAGAC 5 cut(s) 156, 699, 1446, 2130, 2468
BbvI GCAGC 3 cut(s) 703, 1692, 2611
BccI CCATC 5 cut(s) 24, 362, 622, 1535, 2179
BceAI ACGGC 1 cut(s) 1476
BcgI CGANNNNNNTGC 2 cut(s) 1024, 1058
BciT130I CCWGG 8 cut(s) 802, 883, 1197, 1687, 1794, 1885, 2069, 2310
BclI TGATCA 2 cut(s) 22, 2320
BcoDI GTCTC 5 cut(s) 165, 1084, 1820, 2078, 2337
BfaI CTAG 5 cut(s) 783, 819, 1580, 2001, 2465
BfmI CTRYAG 2 cut(s) 1147, 1386
BfoI RGCGCY 1 cut(s) 1466
BfrI CTTAAG 3 cut(s) 157, 1628, 2561
BfuAI ACCTGC 1 cut(s) 2407
BisI GCNGC 7 cut(s) 717, 1066, 1466, 1568, 1681, 2097, 2600
BlsI GCNGC 7 cut(s) 718, 1067, 1467, 1569, 1682, 2098, 2601
Bme1390I CCNGG 8 cut(s) 802, 883, 1197, 1687, 1794, 1885, 2069, 2310
Bme18I GGWCC 3 cut(s) 878, 1796, 2151
BmeRI GACNNNNNGTC 1 cut(s) 700
BmgT120I GGNCC 5 cut(s) 252, 878, 1796, 1861, 2151
BmiI GGNNCC 6 cut(s) 272, 521, 880, 1464, 1485, 1732
BmrFI CCNGG 8 cut(s) 802, 883, 1197, 1687, 1794, 1885, 2069, 2310
BmsI GCATC 8 cut(s) 221, 580, 754, 949, 1529, 1579, 1918, 1944
BoxI GACNNNNGTC 1 cut(s) 2472
BpiI GAAGAC 5 cut(s) 156, 699, 1446, 2130, 2468
BpmI CTGGAG 2 cut(s) 1669, 2292
BsaAI YACGTR 3 cut(s) 631, 917, 987
BsaHI GRCGYC 2 cut(s) 1463, 1889
BsaI GGTCTC 2 cut(s) 165, 2078
BsaWI WCCGGW 2 cut(s) 427, 1100
BsaXI ACNNNNNCTCC 6 cut(s) 1064, 1094, 1715, 1745, 2037, 2067
Bsc4I CCNNNNNNNGG 5 cut(s) 359, 1406, 1727, 2385, 2530
Bse118I RCCGGY 2 cut(s) 427, 1907
Bse1I ACTGG 4 cut(s) 193, 364, 523, 1250
Bse3DI GCAATG 1 cut(s) 92
BseAI TCCGGA 1 cut(s) 1100
BseBI CCWGG 8 cut(s) 802, 883, 1197, 1687, 1794, 1885, 2069, 2310
BseGI GGATG 5 cut(s) 177, 490, 1598, 1930, 2634
BseLI CCNNNNNNNGG 5 cut(s) 359, 1406, 1727, 2385, 2530
BseMI GCAATG 1 cut(s) 92
BseMII CTCAG 4 cut(s) 50, 70, 1293, 2544
BseNI ACTGG 4 cut(s) 193, 364, 523, 1250
BseRI GAGGAG 3 cut(s) 24, 1754, 2570
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 3 cut(s) 703, 1692, 2611
BseYI CCCAGC 1 cut(s) 275
BsgI GTGCAG 1 cut(s) 645
Bsh1236I CGCG 3 cut(s) 1053, 1272, 2471
Bsh1285I CGRYCG 2 cut(s) 64, 2364
BshNI GGYRCC 2 cut(s) 270, 1462
BshTI ACCGGT 1 cut(s) 427
BsiEI CGRYCG 2 cut(s) 64, 2364
BsiSI CCGG 3 cut(s) 428, 1101, 1908
BslFI GGGAC 3 cut(s) 341, 1607, 1703
BslI CCNNNNNNNGG 5 cut(s) 359, 1406, 1727, 2385, 2530
BsmAI GTCTC 5 cut(s) 165, 1084, 1820, 2078, 2337
BsmBI CGTCTC 1 cut(s) 1084
BsmFI GGGAC 3 cut(s) 341, 1607, 1703
BsmI GAATGC 2 cut(s) 1312, 2021
Bso31I GGTCTC 2 cut(s) 165, 2078
Bsp1286I GDGCHC 1 cut(s) 275
Bsp13I TCCGGA 1 cut(s) 1100
Bsp19I CCATGG 1 cut(s) 756
Bsp68I TCGCGA 1 cut(s) 1053
BspACI CCGC 8 cut(s) 203, 250, 868, 1066, 1407, 1466, 1568, 2097
BspCNI CTCAG 4 cut(s) 49, 69, 1294, 2545
BspEI TCCGGA 1 cut(s) 1100
BspFNI CGCG 3 cut(s) 1053, 1272, 2471
BspHI TCATGA 2 cut(s) 19, 2485
BspLI GGNNCC 6 cut(s) 272, 521, 880, 1464, 1485, 1732
BspMI ACCTGC 1 cut(s) 2407
BspPI GGATC 5 cut(s) 514, 527, 1015, 1092, 2210
BspQI GCTCTTC 1 cut(s) 224
BspT107I GGYRCC 2 cut(s) 270, 1462
BspTI CTTAAG 3 cut(s) 157, 1628, 2561
BspTNI GGTCTC 2 cut(s) 165, 2078
BsrBI CCGCTC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 92
BsrFI RCCGGY 2 cut(s) 427, 1907
BsrI ACTGG 4 cut(s) 193, 364, 523, 1250
BssAI RCCGGY 2 cut(s) 427, 1907
BssNI GRCGYC 2 cut(s) 1463, 1889
BssSI CACGAG 2 cut(s) 1332, 1488
BssT1I CCWWGG 7 cut(s) 408, 756, 874, 1726, 1780, 2383, 2412
Bst2BI CACGAG 2 cut(s) 1332, 1488
Bst2UI CCWGG 8 cut(s) 802, 883, 1197, 1687, 1794, 1885, 2069, 2310
Bst4CI ACNGT 4 cut(s) 45, 233, 1151, 1173
Bst6I CTCTTC 2 cut(s) 224, 294
BstACI GRCGYC 2 cut(s) 1463, 1889
BstAFI CTTAAG 3 cut(s) 157, 1628, 2561
BstAPI GCANNNNNTGC 2 cut(s) 725, 2285
BstBAI YACGTR 3 cut(s) 631, 917, 987
BstC8I GCNNGC 8 cut(s) 252, 350, 730, 769, 1933, 2094, 2286, 2290
BstDEI CTNAG 6 cut(s) 36, 56, 1115, 1302, 2163, 2553
BstDSI CCRYGG 2 cut(s) 756, 2038
BstF5I GGATG 5 cut(s) 177, 490, 1598, 1930, 2634
BstFNI CGCG 3 cut(s) 1053, 1272, 2471
BstH2I RGCGCY 1 cut(s) 1466
BstHHI GCGC 6 cut(s) 241, 595, 1035, 1229, 1465, 2604
BstMAI GTCTC 5 cut(s) 165, 1084, 1820, 2078, 2337
BstMCI CGRYCG 2 cut(s) 64, 2364
BstMWI GCNNNNNNNGC 8 cut(s) 110, 209, 247, 725, 941, 2285, 2351, 2616
BstNI CCWGG 8 cut(s) 802, 883, 1197, 1687, 1794, 1885, 2069, 2310
BstNSI RCATGY 3 cut(s) 1935, 2292, 2319
BstPAI GACNNNNGTC 1 cut(s) 2472
BstSCI CCNGG 8 cut(s) 800, 881, 1195, 1685, 1792, 1883, 2067, 2308
BstSFI CTRYAG 2 cut(s) 1147, 1386
BstSLI GKGCMC 1 cut(s) 275
BstUI CGCG 3 cut(s) 1053, 1272, 2471
BstV1I GCAGC 3 cut(s) 703, 1692, 2611
BstV2I GAAGAC 5 cut(s) 156, 699, 1446, 2130, 2468
BstX2I RGATCY 1 cut(s) 519
BstXI CCANNNNNNTGG 1 cut(s) 188
BstYI RGATCY 1 cut(s) 519
BtgI CCRYGG 2 cut(s) 756, 2038
BtgZI GCGATG 1 cut(s) 198
BtsCI GGATG 5 cut(s) 177, 490, 1598, 1930, 2634
BtsIMutI CAGTG 2 cut(s) 87, 357
BtuMI TCGCGA 1 cut(s) 1053
BveI ACCTGC 1 cut(s) 2407
Cac8I GCNNGC 8 cut(s) 252, 350, 730, 769, 1933, 2094, 2286, 2290
CciI TCATGA 2 cut(s) 19, 2485
CfoI GCGC 6 cut(s) 241, 595, 1035, 1229, 1465, 2604
Cfr10I RCCGGY 2 cut(s) 427, 1907
Cfr13I GGNCC 5 cut(s) 252, 878, 1796, 1861, 2151
CseI GACGC 2 cut(s) 1878, 2477
Csp6I GTAC 4 cut(s) 50, 532, 575, 1243
CspAI ACCGGT 1 cut(s) 427
CviQI GTAC 4 cut(s) 50, 532, 575, 1243
DdeI CTNAG 6 cut(s) 36, 56, 1115, 1302, 2163, 2553
DinI GGCGCC 1 cut(s) 1464
DraI TTTAAA 1 cut(s) 2010
DraIII CACNNNGTG 1 cut(s) 917
DrdI GACNNNNNNGTC 1 cut(s) 1448
DriI GACNNNNNGTC 1 cut(s) 700
DseDI GACNNNNNNGTC 1 cut(s) 1448
EaeI YGGCCR 4 cut(s) 835, 895, 942, 2190
Eam1104I CTCTTC 2 cut(s) 224, 294
Eam1105I GACNNNNNGTC 1 cut(s) 700
EarI CTCTTC 2 cut(s) 224, 294
Eco130I CCWWGG 7 cut(s) 408, 756, 874, 1726, 1780, 2383, 2412
Eco147I AGGCCT 1 cut(s) 2619
Eco31I GGTCTC 2 cut(s) 165, 2078
Eco47I GGWCC 3 cut(s) 878, 1796, 2151
Eco57I CTGAAG 4 cut(s) 26, 205, 1655, 2049
Eco72I CACGTG 2 cut(s) 631, 987
EcoO109I RGGNCCY 2 cut(s) 878, 1796
EcoRII CCWGG 8 cut(s) 800, 881, 1195, 1685, 1792, 1883, 2067, 2308
EcoT14I CCWWGG 7 cut(s) 408, 756, 874, 1726, 1780, 2383, 2412
EcoT22I ATGCAT 3 cut(s) 1522, 1933, 1937
EgeI GGCGCC 1 cut(s) 1464
EheI GGCGCC 1 cut(s) 1464
ErhI CCWWGG 7 cut(s) 408, 756, 874, 1726, 1780, 2383, 2412
Esp3I CGTCTC 1 cut(s) 1084
FaqI GGGAC 3 cut(s) 341, 1607, 1703
FauI CCCGC 2 cut(s) 243, 1400
FbaI TGATCA 2 cut(s) 22, 2320
FblI GTMKAC 1 cut(s) 704
Fnu4HI GCNGC 7 cut(s) 717, 1066, 1466, 1568, 1681, 2097, 2600
FokI GGATG 4 cut(s) 164, 497, 1585, 1937
Fsp4HI GCNGC 7 cut(s) 717, 1066, 1466, 1568, 1681, 2097, 2600
FspBI CTAG 5 cut(s) 783, 819, 1580, 2001, 2465
GlaI GCGC 6 cut(s) 240, 594, 1034, 1228, 1464, 2603
GluI GCNGC 7 cut(s) 717, 1066, 1466, 1568, 1681, 2097, 2600
GsaI CCCAGC 1 cut(s) 279
GsuI CTGGAG 2 cut(s) 1669, 2292
HaeII RGCGCY 1 cut(s) 1466
HapII CCGG 3 cut(s) 428, 1101, 1908
HgaI GACGC 2 cut(s) 1878, 2477
HhaI GCGC 6 cut(s) 241, 595, 1035, 1229, 1465, 2604
Hin1I GRCGYC 2 cut(s) 1463, 1889
Hin6I GCGC 6 cut(s) 239, 593, 1033, 1227, 1463, 2602
HinP1I GCGC 6 cut(s) 239, 593, 1033, 1227, 1463, 2602
HincII GTYRAC 4 cut(s) 1504, 2396, 2547, 2580
HindII GTYRAC 4 cut(s) 1504, 2396, 2547, 2580
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 9 cut(s) 656, 701, 1134, 1330, 1595, 2083, 2408, 2473, 2536
HpaII CCGG 3 cut(s) 428, 1101, 1908
HphI GGTGA 5 cut(s) 514, 632, 1339, 2002, 2026
Hpy166II GTNNAC 7 cut(s) 575, 705, 1504, 1993, 2396, 2547, 2580
Hpy188I TCNGA 6 cut(s) 39, 779, 1560, 1603, 2029, 2078
Hpy188III TCNNGA 9 cut(s) 20, 1052, 1101, 1187, 1490, 1580, 1825, 2486, 2540
Hpy8I GTNNAC 7 cut(s) 575, 705, 1504, 1993, 2396, 2547, 2580
Hpy99I CGWCG 3 cut(s) 377, 1790, 1826
HpyCH4III ACNGT 4 cut(s) 45, 233, 1151, 1173
HpyF10VI GCNNNNNNNGC 8 cut(s) 110, 209, 247, 725, 941, 2285, 2351, 2616
HpyF3I CTNAG 6 cut(s) 36, 56, 1115, 1302, 2163, 2553
Hsp92I GRCGYC 2 cut(s) 1463, 1889
HspAI GCGC 6 cut(s) 239, 593, 1033, 1227, 1463, 2602
KasI GGCGCC 1 cut(s) 1462
Kpn2I TCCGGA 1 cut(s) 1100
Ksp22I TGATCA 2 cut(s) 22, 2320
LguI GCTCTTC 1 cut(s) 224
LmnI GCTCC 7 cut(s) 37, 1373, 1688, 1736, 2431, 2446, 2557
Lsp1109I GCAGC 3 cut(s) 703, 1692, 2611
LweI GCATC 8 cut(s) 221, 580, 754, 949, 1529, 1579, 1918, 1944
MaeI CTAG 5 cut(s) 783, 819, 1580, 2001, 2465
MaeIII GTNAC 8 cut(s) 1178, 1327, 1410, 1450, 1622, 1890, 2032, 2260
MbiI CCGCTC 1 cut(s) 203
MfeI CAATTG 1 cut(s) 2403
MflI RGATCY 1 cut(s) 519
MhlI GDGCHC 1 cut(s) 275
MlsI TGGCCA 1 cut(s) 837
MluNI TGGCCA 1 cut(s) 837
Mly113I GGCGCC 1 cut(s) 1463
MlyI GAGTC 6 cut(s) 710, 1143, 1324, 1589, 2417, 2482
MmeI TCCRAC 2 cut(s) 802, 2424
Mox20I TGGCCA 1 cut(s) 837
Mph1103I ATGCAT 3 cut(s) 1522, 1933, 1937
MroI TCCGGA 1 cut(s) 1100
MroXI GAANNNNTTC 1 cut(s) 381
MscI TGGCCA 1 cut(s) 837
MseI TTAA 8 cut(s) 158, 773, 1206, 1629, 1965, 2009, 2199, 2562
Msp20I TGGCCA 1 cut(s) 837
MspCI CTTAAG 3 cut(s) 157, 1628, 2561
MspI CCGG 3 cut(s) 428, 1101, 1908
MspR9I CCNGG 8 cut(s) 802, 883, 1197, 1687, 1794, 1885, 2069, 2310
MunI CAATTG 1 cut(s) 2403
Mva1269I GAATGC 2 cut(s) 1312, 2021
MvaI CCWGG 8 cut(s) 802, 883, 1197, 1687, 1794, 1885, 2069, 2310
MvnI CGCG 3 cut(s) 1053, 1272, 2471
MwoI GCNNNNNNNGC 8 cut(s) 110, 209, 247, 725, 941, 2285, 2351, 2616
NarI GGCGCC 1 cut(s) 1463
NcoI CCATGG 1 cut(s) 756
NlaIV GGNNCC 6 cut(s) 272, 521, 880, 1464, 1485, 1732
NmeAIII GCCGAG 1 cut(s) 873
NmuCI GTSAC 5 cut(s) 1327, 1410, 1450, 1890, 2032
NruI TCGCGA 1 cut(s) 1053
NsiI ATGCAT 3 cut(s) 1522, 1933, 1937
NspI RCATGY 3 cut(s) 1935, 2292, 2319
PaeI GCATGC 2 cut(s) 1935, 2292
PagI TCATGA 2 cut(s) 19, 2485
PasI CCCWGGG 1 cut(s) 882
PceI AGGCCT 1 cut(s) 2619
PciI ACATGT 1 cut(s) 2315
PciSI GCTCTTC 1 cut(s) 224
PcsI WCGNNNNNNNCGW 3 cut(s) 1050, 1165, 2141
PctI GAATGC 2 cut(s) 1312, 2021
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 3 cut(s) 656, 2083, 2536
PflFI GACNNNGTC 1 cut(s) 1076
PfoI TCCNGGA 1 cut(s) 1195
PinAI ACCGGT 1 cut(s) 427
PkrI GCNGC 7 cut(s) 718, 1067, 1467, 1569, 1682, 2098, 2601
Ple19I CGATCG 2 cut(s) 64, 2364
PleI GAGTC 6 cut(s) 709, 1142, 1324, 1589, 2416, 2481
PluTI GGCGCC 1 cut(s) 1466
PmaCI CACGTG 2 cut(s) 631, 987
PmlI CACGTG 2 cut(s) 631, 987
PpsI GAGTC 6 cut(s) 709, 1142, 1324, 1589, 2416, 2481
Ppu21I YACGTR 3 cut(s) 631, 917, 987
PpuMI RGGWCCY 2 cut(s) 878, 1796
PscI ACATGT 1 cut(s) 2315
PshAI GACNNNNGTC 1 cut(s) 2472
Psp1406I AACGTT 2 cut(s) 130, 2326
Psp5II RGGWCCY 2 cut(s) 878, 1796
Psp6I CCWGG 8 cut(s) 800, 881, 1195, 1685, 1792, 1883, 2067, 2308
PspCI CACGTG 2 cut(s) 631, 987
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 8 cut(s) 800, 881, 1195, 1685, 1792, 1883, 2067, 2308
PspN4I GGNNCC 6 cut(s) 272, 521, 880, 1464, 1485, 1732
PspPI GGNCC 5 cut(s) 252, 878, 1796, 1861, 2151
PspPPI RGGWCCY 2 cut(s) 878, 1796
PsuI RGATCY 1 cut(s) 519
PsyI GACNNNGTC 1 cut(s) 1076
PvuI CGATCG 2 cut(s) 64, 2364
RruI TCGCGA 1 cut(s) 1053
RsaI GTAC 4 cut(s) 51, 533, 576, 1244
RsaNI GTAC 4 cut(s) 50, 532, 575, 1243
SapI GCTCTTC 1 cut(s) 224
SaqAI TTAA 8 cut(s) 158, 773, 1206, 1629, 1965, 2009, 2199, 2562
SatI GCNGC 7 cut(s) 717, 1066, 1466, 1568, 1681, 2097, 2600
Sau96I GGNCC 5 cut(s) 252, 878, 1796, 1861, 2151
SchI GAGTC 6 cut(s) 710, 1143, 1324, 1589, 2417, 2482
ScrFI CCNGG 8 cut(s) 802, 883, 1197, 1687, 1794, 1885, 2069, 2310
SduI GDGCHC 1 cut(s) 275
SfaNI GCATC 8 cut(s) 221, 580, 754, 949, 1529, 1579, 1918, 1944
SfcI CTRYAG 2 cut(s) 1147, 1386
SfoI GGCGCC 1 cut(s) 1464
SinI GGWCC 3 cut(s) 878, 1796, 2151
SmlI CTYRAG 3 cut(s) 157, 1628, 2561
SmoI CTYRAG 3 cut(s) 157, 1628, 2561
SphI GCATGC 2 cut(s) 1935, 2292
SseBI AGGCCT 1 cut(s) 2619
SsiI CCGC 8 cut(s) 203, 250, 868, 1066, 1407, 1466, 1568, 2097
SspDI GGCGCC 1 cut(s) 1462
SspMI CTAG 5 cut(s) 783, 819, 1580, 2001, 2465
StuI AGGCCT 1 cut(s) 2619
StyD4I CCNGG 8 cut(s) 800, 881, 1195, 1685, 1792, 1883, 2067, 2308
StyI CCWWGG 7 cut(s) 408, 756, 874, 1726, 1780, 2383, 2412
TaaI ACNGT 4 cut(s) 45, 233, 1151, 1173
TaqI TCGA 6 cut(s) 199, 393, 1096, 1824, 2360, 2364
TatI WGTACW 1 cut(s) 1242
TauI GCSGC 4 cut(s) 1068, 1468, 1570, 2099
TfiI GAWTC 3 cut(s) 656, 2083, 2536
Tru1I TTAA 8 cut(s) 158, 773, 1206, 1629, 1965, 2009, 2199, 2562
Tru9I TTAA 8 cut(s) 158, 773, 1206, 1629, 1965, 2009, 2199, 2562
TscAI CASTG 2 cut(s) 87, 364
TseFI GTSAC 5 cut(s) 1327, 1410, 1450, 1890, 2032
TseI GCWGC 3 cut(s) 716, 1680, 2599
Tsp45I GTSAC 5 cut(s) 1327, 1410, 1450, 1890, 2032
TspDTI ATGAA 7 cut(s) 736, 1709, 1770, 1821, 1965, 2211, 2474
TspGWI ACGGA 4 cut(s) 140, 592, 2354, 2360
TspRI CASTG 2 cut(s) 87, 364
Tth111I GACNNNGTC 1 cut(s) 1076
Vha464I CTTAAG 3 cut(s) 157, 1628, 2561
VpaK11BI GGWCC 3 cut(s) 878, 1796, 2151
XapI RAATTY 3 cut(s) 398, 1757, 2419
XbaI TCTAGA 1 cut(s) 1579
XceI RCATGY 3 cut(s) 1935, 2292, 2319
XmiI GTMKAC 1 cut(s) 704
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 5 cut(s) 783, 819, 1580, 2001, 2465
Zsp2I ATGCAT 3 cut(s) 1522, 1933, 1937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.