pycom04g09610

spliceosomal complex assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000179
Physical Location & Seq
Forward (+)
12353074 .. 12354965
1892 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g09610.1

Sequence Viewer

Length: 891 bp
ATGGCTGCAGGTGAATCTTTCAATCTTACTATCCCGGTCTTGGCCAATATTTATAACGACTTGAATGTTGTTTCGAACTTGGCAAACACTGAAGATCGTGTTGCGGTTCTTCCCTACCACTATGTGCATGGTTGGTTGGGTATTTATTCTTATACGGAGATGCTATTTGGAGGTGACCTACCTACAGGCGGGGAGATTGGGGATCCACCTGGTACAGAGCTTGTCCCAAGTCCGTCAAGTTTCCCAAGTTTGCCATGTGTAGGTGGATGCATGCAAGATCCCGTCATGCATTCTATACCTTTGCCAGCTAGCTCTGAGATCTCTCTAAGAGGGCCAAACCTTAGTGGTACTGAGCAGCTTATTCATCGTATCAATCTTCGTGCGACAATAGATATCAAGAGCCATATTGAGGGGAGGATTTCAAAATGCCCGTCGGAGGACCTTGCGTTGCTTAAGGAAGACTTGTCGAAACTTGTTTATGCAATTGACAATCTTAACATTGATTCTTCATCCTTGAAGATCAAGATTGCCGAACTTATGGCCGCCTCAACTGAGTATTCTTCCTTGCGTGTTATTTCTTCGAAGAAGTTGAGTCCAGATGTTAGAGCTCACCAACTTGCTGCAATAGACTTGTCACTAACCCAAGCTCGGTCTTCTCAGCAAGCTGCTTCGGGAGATTATCAATCCACAGAGAATTCTTTAGCTTCCGTTCAGGTACGTCTAGATACGTTGATGCGAGAGCAAGAACAGCTGGGCATCGAGGCATCAAAACTTGAAAGTGTTCTCGGGGAGCAGGGAGCTACACTTTCTCAATGCCAAGAGGAGATTTCATGCCTAGAACAAGAGAAAGACATGGCTATGGAGTTGCCCATCCTGTCTCTCACTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve zf-CCHC

Protein Analysis

297

Amino Acids

32.01

Weight (kDa)

4.63

Isoelectric Point (pI)

62.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000179)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18561 FvH4_1g29681 FvH4_2g15190 FvH4_3g22800 FvH4_3g22801 FvH4_3g26700 FvH4_4g15162 FvH4_4g15163 FvH4_4g15164 FvH4_4g22273 FvH4_7g02320 FvH4_7g03670 FvH4_7g03671
malus_domestica MD07G1097100.v1.1 MD11G1208600.v1.1 MD15G1303300.v1.1
prunus_persica Prupe.2G070800_v2.0.a1
pyrus_communis pycom02g13430 pycom04g06950 pycom04g09420 pycom04g09610 pycom06g05740 pycom07g00010 pycom07g06720 pycom07g09960 pycom07g10670 pycom07g10680 pycom07g10920 pycom07g27810 pycom08g12110 pycom08g13640 pycom09g03570 pycom09g13470 pycom09g13510 pycom10g01180 pycom10g02180 pycom10g06840 pycom12424g00130 pycom12g03090 pycom12g09570 pycom12g09580 pycom12g09590 pycom1310g00020 pycom13g25030 pycom13g25310 pycom13g25700 pycom13g26160 pycom13g27790 pycom13g28780 pycom13g28790 pycom1683g00070 pycom16g19330 pycom16g21210 pycom16g21710 pycom16g23400 pycom16g24730 pycom17g17550 pycom17g27670 pycom420g00110 pycom420g00130 pycom420g00610 pycom520g00600 pycom675g00050
rosa_chinensis RchiOBHm_Chr1g0316731
rosa_laevigata RLG00000030467
rosa_multiflora Rmu_co8112106.1_g000001 Rmu_co8301911.1_g000001 Rmu_sc0000161.1_g000042 Rmu_sc0000161.1_g000043 Rmu_sc0000173.1_g000035 Rmu_sc0000173.1_g000036 Rmu_sc0000480.1_g000024 Rmu_sc0000670.1_g000020 Rmu_sc0000670.1_g000021 Rmu_sc0000962.1_g000043 Rmu_sc0000962.1_g000044 Rmu_sc0001025.1_g000004 Rmu_sc0001075.1_g000026 Rmu_sc0001075.1_g000027 Rmu_sc0001167.1_g000050 Rmu_sc0001227.1_g000043 Rmu_sc0001346.1_g000006 Rmu_sc0001438.1_g000034 Rmu_sc0001946.1_g000002 Rmu_sc0001981.1_g000005 Rmu_sc0002106.1_g000005 Rmu_sc0002187.1_g000004 Rmu_sc0002187.1_g000005 Rmu_sc0002187.1_g000018 Rmu_sc0002539.1_g000097 Rmu_sc0003008.1_g000050 Rmu_sc0003008.1_g000051 Rmu_sc0003069.1_g000033 Rmu_sc0003069.1_g000034 Rmu_sc0003412.1_g000016 Rmu_sc0003553.1_g000005 Rmu_sc0003553.1_g000006 Rmu_sc0003641.1_g000025 Rmu_sc0003652.1_g000006 Rmu_sc0003687.1_g000019 Rmu_sc0003687.1_g000020 Rmu_sc0004234.1_g000013 Rmu_sc0004249.1_g000005 Rmu_sc0005044.1_g000027 Rmu_sc0005060.1_g000012 Rmu_sc0005816.1_g000005 Rmu_sc0006175.1_g000006 Rmu_sc0006175.1_g000007 Rmu_sc0006695.1_g000046 Rmu_sc0006725.1_g000014 Rmu_sc0006746.1_g000015 Rmu_sc0006746.1_g000016 Rmu_sc0006912.1_g000008 Rmu_sc0007355.1_g000004 Rmu_sc0007705.1_g000001 Rmu_sc0007903.1_g000003 Rmu_sc0007953.1_g000001 Rmu_sc0008630.1_g000001 Rmu_sc0008923.1_g000018 Rmu_sc0008923.1_g000019 Rmu_sc0009186.1_g000003 Rmu_sc0010184.1_g000007 Rmu_sc0010243.1_g000001 Rmu_sc0011242.1_g000004 Rmu_sc0012849.1_g000001 Rmu_sc0012849.1_g000002 Rmu_sc0012883.1_g000001 Rmu_sc0013028.1_g000015 Rmu_sc0015042.1_g000003 Rmu_sc0016730.1_g000002 Rmu_sc0017702.1_g000002 Rmu_sc0017791.1_g000007 Rmu_sc0017874.1_g000005 Rmu_sc0025334.1_g000001 Rmu_sc0025334.1_g000002 Rmu_sc0028789.1_g000001 Rmu_sc0030538.1_g000001 Rmu_sc0033137.1_g000001 Rmu_ssc0000183.1_g000013 Rmu_ssc0000259.1_g000061 Rmu_ssc0000259.1_g000062 Rmu_ssc0000259.1_g000063
rosa_roxburghii Rroxscaffold_173G00435030 Rroxscaffold_173G00435040 Rroxscaffold_1G00033030 Rroxscaffold_2G00093360 Rroxscaffold_2G00113080 Rroxscaffold_2G00146180 Rroxscaffold_3G00227920 Rroxscaffold_3G00251750 Rroxscaffold_4G00328240 Rroxscaffold_5G00333920 Rroxscaffold_5G00338240 Rroxscaffold_5G00342700 Rroxscaffold_6G00412810 Rroxscaffold_6G00423130 Rroxscaffold_7G00175190
rosa_rugosa Rorug01G0029200 Rorug01G0029300 Rorug01G0029400 Rorug01G0029400
rosa_samantha Rh1AG040900 Rh1BG038100 Rh1CG042000 Rh1DG027900
rosa_wichuraiana Rw0G019970 Rw1G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 54
AciI CCGC 3 cut(s) 104, 189, 543
AclWI GGATC 3 cut(s) 197, 210, 272
AcoI YGGCCR 2 cut(s) 42, 540
AcsI RAATTY 1 cut(s) 694
AcuI CTGAAG 1 cut(s) 111
AdeI CACNNNGTG 1 cut(s) 124
AfaI GTAC 3 cut(s) 214, 349, 717
AfiI CCNNNNNNNGG 6 cut(s) 40, 188, 260, 409, 436, 648
AflII CTTAAG 1 cut(s) 452
AgsI TTSAA 5 cut(s) 22, 64, 423, 517, 776
AjnI CCWGG 1 cut(s) 208
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 1 cut(s) 882
AlwI GGATC 3 cut(s) 197, 210, 272
Ama87I CYCGRG 1 cut(s) 785
AoxI GGCC 3 cut(s) 42, 332, 540
ApeKI GCWGC 4 cut(s) 5, 355, 620, 665
ApoI RAATTY 1 cut(s) 694
ArsI GACNNNNNNTTYG 2 cut(s) 416, 448
Asp700I GAANNNNTTC 1 cut(s) 780
AspS9I GGNCC 2 cut(s) 332, 439
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 3 cut(s) 23, 185, 602
AsuII TTCGAA 2 cut(s) 74, 581
AsuNHI GCTAGC 1 cut(s) 308
AvaI CYCGRG 1 cut(s) 785
AvaII GGWCC 1 cut(s) 439
BalI TGGCCA 1 cut(s) 44
BamHI GGATCC 1 cut(s) 202
BanII GRGCYC 1 cut(s) 610
BbsI GAAGAC 2 cut(s) 465, 645
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 3 cut(s) 367, 607, 652
BccI CCATC 1 cut(s) 878
BciT130I CCWGG 1 cut(s) 210
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 1 cut(s) 882
BfaI CTAG 3 cut(s) 309, 722, 836
BfmI CTRYAG 2 cut(s) 6, 183
BfrI CTTAAG 1 cut(s) 452
BglII AGATCT 1 cut(s) 318
BisI GCNGC 5 cut(s) 6, 356, 543, 621, 666
BlsI GCNGC 5 cut(s) 7, 357, 544, 622, 667
Bme1390I CCNGG 2 cut(s) 35, 210
Bme18I GGWCC 1 cut(s) 439
BmeT110I CYCGRG 1 cut(s) 785
BmgT120I GGNCC 2 cut(s) 332, 439
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 2 cut(s) 35, 210
BmsI GCATC 5 cut(s) 150, 257, 723, 765, 773
BmtI GCTAGC 1 cut(s) 312
BpiI GAAGAC 2 cut(s) 465, 645
Bpu14I TTCGAA 2 cut(s) 74, 581
BpuMI CCSGG 1 cut(s) 35
Bsc4I CCNNNNNNNGG 6 cut(s) 40, 188, 260, 409, 436, 648
BseBI CCWGG 1 cut(s) 210
BseGI GGATG 3 cut(s) 272, 509, 870
BseLI CCNNNNNNNGG 6 cut(s) 40, 188, 260, 409, 436, 648
BseMII CTCAG 5 cut(s) 306, 342, 543, 671, 876
BseRI GAGGAG 1 cut(s) 836
BseXI GCAGC 3 cut(s) 367, 607, 652
BseYI CCCAGC 1 cut(s) 751
BshFI GGCC 3 cut(s) 44, 334, 542
BsiHKAI GWGCWC 1 cut(s) 610
BsiHKCI CYCGRG 1 cut(s) 785
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 6 cut(s) 40, 188, 260, 409, 436, 648
BsmAI GTCTC 1 cut(s) 882
BsmFI GGGAC 1 cut(s) 209
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 3 cut(s) 44, 334, 542
BsoBI CYCGRG 1 cut(s) 785
Bsp119I TTCGAA 2 cut(s) 74, 581
Bsp1286I GDGCHC 1 cut(s) 610
Bsp143I GATC 5 cut(s) 94, 202, 277, 318, 519
BspACI CCGC 3 cut(s) 104, 189, 543
BspANI GGCC 3 cut(s) 44, 334, 542
BspCNI CTCAG 5 cut(s) 307, 343, 544, 670, 877
BspLI GGNNCC 1 cut(s) 204
BspMAI CTGCAG 1 cut(s) 10
BspOI GCTAGC 1 cut(s) 312
BspPI GGATC 3 cut(s) 197, 210, 272
BspT104I TTCGAA 2 cut(s) 74, 581
BspTI CTTAAG 1 cut(s) 452
BssMI GATC 5 cut(s) 94, 202, 277, 318, 519
Bst2UI CCWGG 1 cut(s) 210
BstAFI CTTAAG 1 cut(s) 452
BstBI TTCGAA 2 cut(s) 74, 581
BstC8I GCNNGC 4 cut(s) 272, 306, 310, 663
BstDEI CTNAG 7 cut(s) 315, 326, 341, 351, 552, 657, 885
BstEII GGTNACC 1 cut(s) 173
BstF5I GGATG 3 cut(s) 272, 509, 870
BstKTI GATC 5 cut(s) 97, 205, 280, 321, 522
BstMAI GTCTC 1 cut(s) 882
BstMBI GATC 5 cut(s) 94, 202, 277, 318, 519
BstMWI GCNNNNNNNGC 1 cut(s) 748
BstNI CCWGG 1 cut(s) 210
BstNSI RCATGY 1 cut(s) 274
BstPI GGTNACC 1 cut(s) 173
BstSCI CCNGG 2 cut(s) 33, 208
BstSFI CTRYAG 2 cut(s) 6, 183
BstV1I GCAGC 3 cut(s) 367, 607, 652
BstV2I GAAGAC 2 cut(s) 465, 645
BstX2I RGATCY 3 cut(s) 202, 277, 318
BstYI RGATCY 3 cut(s) 202, 277, 318
BsuRI GGCC 3 cut(s) 44, 334, 542
BtsCI GGATG 3 cut(s) 272, 509, 870
BtsIMutI CAGTG 2 cut(s) 87, 882
Cac8I GCNNGC 4 cut(s) 272, 306, 310, 663
Cfr13I GGNCC 2 cut(s) 332, 439
CsiI ACCWGGT 1 cut(s) 208
Csp6I GTAC 3 cut(s) 213, 348, 716
CviAII CATG 6 cut(s) 128, 255, 271, 286, 831, 853
CviQI GTAC 3 cut(s) 213, 348, 716
DdeI CTNAG 7 cut(s) 315, 326, 341, 351, 552, 657, 885
DpnI GATC 5 cut(s) 96, 204, 279, 320, 521
DpnII GATC 5 cut(s) 94, 202, 277, 318, 519
DraIII CACNNNGTG 1 cut(s) 124
EaeI YGGCCR 2 cut(s) 42, 540
Ecl136II GAGCTC 1 cut(s) 608
Eco24I GRGCYC 1 cut(s) 610
Eco32I GATATC 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 439
Eco53kI GAGCTC 1 cut(s) 608
Eco57I CTGAAG 1 cut(s) 111
Eco88I CYCGRG 1 cut(s) 785
Eco91I GGTNACC 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 608
EcoO109I RGGNCCY 1 cut(s) 439
EcoO65I GGTNACC 1 cut(s) 173
EcoRI GAATTC 1 cut(s) 694
EcoRII CCWGG 1 cut(s) 208
EcoRV GATATC 1 cut(s) 394
EcoT22I ATGCAT 2 cut(s) 272, 291
EcoT38I GRGCYC 1 cut(s) 610
FaeI CATG 6 cut(s) 131, 258, 274, 289, 834, 856
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 1 cut(s) 209
FatI CATG 6 cut(s) 127, 254, 270, 285, 830, 852
FauI CCCGC 1 cut(s) 182
Fnu4HI GCNGC 5 cut(s) 6, 356, 543, 621, 666
FokI GGATG 3 cut(s) 279, 496, 857
FriOI GRGCYC 1 cut(s) 610
Fsp4HI GCNGC 5 cut(s) 6, 356, 543, 621, 666
FspBI CTAG 3 cut(s) 309, 722, 836
GluI GCNGC 5 cut(s) 6, 356, 543, 621, 666
GsaI CCCAGC 1 cut(s) 755
HaeIII GGCC 3 cut(s) 44, 334, 542
HapII CCGG 1 cut(s) 35
Hin1II CATG 6 cut(s) 131, 258, 274, 289, 834, 856
HinfI GANTC 3 cut(s) 14, 503, 592
HpaII CCGG 1 cut(s) 35
HphI GGTGA 3 cut(s) 23, 185, 602
Hpy188I TCNGA 2 cut(s) 316, 436
Hpy188III TCNNGA 5 cut(s) 397, 523, 596, 672, 722
Hpy99I CGWCG 1 cut(s) 436
HpyCH4IV ACGT 2 cut(s) 718, 728
HpyCH4V TGCA 7 cut(s) 8, 127, 270, 274, 289, 482, 623
HpyF10VI GCNNNNNNNGC 1 cut(s) 748
HpyF3I CTNAG 7 cut(s) 315, 326, 341, 351, 552, 657, 885
HpySE526I ACGT 2 cut(s) 718, 728
Hsp92II CATG 6 cut(s) 131, 258, 274, 289, 834, 856
Kzo9I GATC 5 cut(s) 94, 202, 277, 318, 519
LmnI GCTCC 2 cut(s) 790, 797
Lsp1109I GCAGC 3 cut(s) 367, 607, 652
LweI GCATC 5 cut(s) 150, 257, 723, 765, 773
MabI ACCWGGT 1 cut(s) 208
MaeI CTAG 3 cut(s) 309, 722, 836
MaeII ACGT 2 cut(s) 718, 728
MaeIII GTNAC 2 cut(s) 173, 633
MalI GATC 5 cut(s) 96, 204, 279, 320, 521
MboI GATC 5 cut(s) 94, 202, 277, 318, 519
MfeI CAATTG 1 cut(s) 483
MflI RGATCY 3 cut(s) 202, 277, 318
MhlI GDGCHC 1 cut(s) 610
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 2 cut(s) 483, 694
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 1 cut(s) 601
MmeI TCCRAC 1 cut(s) 414
MnlI CCTC 8 cut(s) 164, 323, 403, 408, 430, 556, 754, 814
Mox20I TGGCCA 1 cut(s) 44
Mph1103I ATGCAT 2 cut(s) 272, 291
MroXI GAANNNNTTC 1 cut(s) 780
MscI TGGCCA 1 cut(s) 44
MseI TTAA 2 cut(s) 453, 495
MslI CAYNNNNRTG 1 cut(s) 857
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 751
MspCI CTTAAG 1 cut(s) 452
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 2 cut(s) 35, 210
MunI CAATTG 1 cut(s) 483
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 748
NciI CCSGG 1 cut(s) 35
NdeII GATC 5 cut(s) 94, 202, 277, 318, 519
NheI GCTAGC 1 cut(s) 308
NlaIII CATG 6 cut(s) 131, 258, 274, 289, 834, 856
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 2 cut(s) 173, 633
NsiI ATGCAT 2 cut(s) 272, 291
NspI RCATGY 1 cut(s) 274
NspV TTCGAA 2 cut(s) 74, 581
PaeI GCATGC 1 cut(s) 274
PctI GAATGC 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 780
PfeI GAWTC 2 cut(s) 14, 503
PkrI GCNGC 5 cut(s) 7, 357, 544, 622, 667
PleI GAGTC 1 cut(s) 600
PpsI GAGTC 1 cut(s) 600
PpuMI RGGWCCY 1 cut(s) 439
PsiI TTATAA 1 cut(s) 54
Psp124BI GAGCTC 1 cut(s) 610
Psp5II RGGWCCY 1 cut(s) 439
Psp6I CCWGG 1 cut(s) 208
PspEI GGTNACC 1 cut(s) 173
PspFI CCCAGC 1 cut(s) 751
PspGI CCWGG 1 cut(s) 208
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 2 cut(s) 332, 439
PspPPI RGGWCCY 1 cut(s) 439
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 3 cut(s) 202, 277, 318
PvuII CAGCTG 1 cut(s) 751
RsaI GTAC 3 cut(s) 214, 349, 717
RsaNI GTAC 3 cut(s) 213, 348, 716
RseI CAYNNNNRTG 1 cut(s) 857
SacI GAGCTC 1 cut(s) 610
SaqAI TTAA 2 cut(s) 453, 495
SatI GCNGC 5 cut(s) 6, 356, 543, 621, 666
Sau3AI GATC 5 cut(s) 94, 202, 277, 318, 519
Sau96I GGNCC 2 cut(s) 332, 439
SchI GAGTC 1 cut(s) 601
ScrFI CCNGG 2 cut(s) 35, 210
SduI GDGCHC 1 cut(s) 610
SexAI ACCWGGT 1 cut(s) 208
SfaNI GCATC 5 cut(s) 150, 257, 723, 765, 773
SfcI CTRYAG 2 cut(s) 6, 183
SfuI TTCGAA 2 cut(s) 74, 581
SinI GGWCC 1 cut(s) 439
SmiMI CAYNNNNRTG 1 cut(s) 857
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
SphI GCATGC 1 cut(s) 274
Sse9I AATT 2 cut(s) 483, 694
SsiI CCGC 3 cut(s) 104, 189, 543
SspI AATATT 1 cut(s) 49
SspMI CTAG 3 cut(s) 309, 722, 836
SstI GAGCTC 1 cut(s) 610
StyD4I CCNGG 2 cut(s) 33, 208
TaiI ACGT 2 cut(s) 721, 731
TaqI TCGA 4 cut(s) 74, 467, 581, 759
TaqII GACCGA 1 cut(s) 639
TasI AATT 2 cut(s) 483, 694
TauI GCSGC 1 cut(s) 545
TfiI GAWTC 2 cut(s) 14, 503
Tru1I TTAA 2 cut(s) 453, 495
Tru9I TTAA 2 cut(s) 453, 495
TscAI CASTG 2 cut(s) 94, 889
TseFI GTSAC 2 cut(s) 173, 633
TseI GCWGC 4 cut(s) 5, 355, 620, 665
Tsp45I GTSAC 2 cut(s) 173, 633
TspDTI ATGAA 3 cut(s) 353, 498, 819
TspGWI ACGGA 3 cut(s) 170, 222, 697
TspRI CASTG 2 cut(s) 94, 889
Vha464I CTTAAG 1 cut(s) 452
VpaK11BI GGWCC 1 cut(s) 439
XapI RAATTY 1 cut(s) 694
XbaI TCTAGA 1 cut(s) 721
XceI RCATGY 1 cut(s) 274
XcmI CCANNNNNNNNNTGG 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 780
XspI CTAG 3 cut(s) 309, 722, 836
Zsp2I ATGCAT 2 cut(s) 272, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.