NatA auxiliary
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 172 |
| AccB1I | GGYRCC | 1 cut(s) | 172 |
| AccI | GTMKAC | 1 cut(s) | 307 |
| AccIII | TCCGGA | 1 cut(s) | 52 |
| AclWI | GGATC | 3 cut(s) | 44, 57, 274 |
| AcsI | RAATTY | 1 cut(s) | 371 |
| AcuI | CTGAAG | 2 cut(s) | 126, 266 |
| AfaI | GTAC | 1 cut(s) | 174 |
| AflII | CTTAAG | 1 cut(s) | 400 |
| AgsI | TTSAA | 2 cut(s) | 22, 289 |
| AluBI | AGCT | 4 cut(s) | 146, 184, 188, 292 |
| AluI | AGCT | 4 cut(s) | 146, 184, 188, 292 |
| Alw21I | GWGCWC | 1 cut(s) | 93 |
| Alw26I | GTCTC | 1 cut(s) | 210 |
| Alw44I | GTGCAC | 1 cut(s) | 89 |
| AlwI | GGATC | 3 cut(s) | 44, 57, 274 |
| Aor13HI | TCCGGA | 1 cut(s) | 52 |
| AoxI | GGCC | 1 cut(s) | 107 |
| ApaLI | GTGCAC | 1 cut(s) | 89 |
| ApoI | RAATTY | 1 cut(s) | 371 |
| Asp718I | GGTACC | 1 cut(s) | 172 |
| AspS9I | GGNCC | 1 cut(s) | 107 |
| AsuHPI | GGTGA | 2 cut(s) | 28, 170 |
| AsuNHI | GCTAGC | 1 cut(s) | 184 |
| BaeGI | GKGCMC | 1 cut(s) | 93 |
| BamHI | GGATCC | 1 cut(s) | 49 |
| BanI | GGYRCC | 1 cut(s) | 172 |
| BbsI | GAAGAC | 1 cut(s) | 243 |
| Bbv12I | GWGCWC | 1 cut(s) | 93 |
| BccI | CCATC | 2 cut(s) | 47, 65 |
| BclI | TGATCA | 1 cut(s) | 262 |
| BcoDI | GTCTC | 1 cut(s) | 210 |
| BfaI | CTAG | 2 cut(s) | 185, 415 |
| BfrI | CTTAAG | 1 cut(s) | 400 |
| BglII | AGATCT | 1 cut(s) | 206 |
| BmgT120I | GGNCC | 1 cut(s) | 107 |
| BmiI | GGNNCC | 2 cut(s) | 51, 174 |
| BmrI | ACTGGG | 2 cut(s) | 114, 388 |
| BmsI | GCATC | 1 cut(s) | 265 |
| BmtI | GCTAGC | 1 cut(s) | 188 |
| BmuI | ACTGGG | 2 cut(s) | 114, 388 |
| BpiI | GAAGAC | 1 cut(s) | 243 |
| BpmI | CTGGAG | 1 cut(s) | 140 |
| BpuEI | CTTGAG | 1 cut(s) | 96 |
| Bsa29I | ATCGAT | 1 cut(s) | 120 |
| BsaI | GGTCTC | 1 cut(s) | 210 |
| BsaWI | WCCGGW | 1 cut(s) | 52 |
| Bse1I | ACTGG | 2 cut(s) | 109, 383 |
| Bse3DI | GCAATG | 1 cut(s) | 340 |
| BseAI | TCCGGA | 1 cut(s) | 52 |
| BseCI | ATCGAT | 1 cut(s) | 120 |
| BseGI | GGATG | 1 cut(s) | 150 |
| BseMI | GCAATG | 1 cut(s) | 340 |
| BseMII | CTCAG | 3 cut(s) | 194, 290, 310 |
| BseNI | ACTGG | 2 cut(s) | 109, 383 |
| BseRI | GAGGAG | 1 cut(s) | 264 |
| BseSI | GKGCMC | 1 cut(s) | 93 |
| Bsh1285I | CGRYCG | 1 cut(s) | 304 |
| BshFI | GGCC | 1 cut(s) | 109 |
| BshNI | GGYRCC | 1 cut(s) | 172 |
| BshVI | ATCGAT | 1 cut(s) | 120 |
| BsiEI | CGRYCG | 1 cut(s) | 304 |
| BsiHKAI | GWGCWC | 1 cut(s) | 93 |
| BsiSI | CCGG | 1 cut(s) | 53 |
| BslFI | GGGAC | 2 cut(s) | 43, 110 |
| BsmAI | GTCTC | 1 cut(s) | 210 |
| BsmFI | GGGAC | 2 cut(s) | 43, 110 |
| BsnI | GGCC | 1 cut(s) | 109 |
| Bso31I | GGTCTC | 1 cut(s) | 210 |
| Bsp1286I | GDGCHC | 1 cut(s) | 93 |
| Bsp13I | TCCGGA | 1 cut(s) | 52 |
| Bsp143I | GATC | 5 cut(s) | 49, 206, 262, 279, 301 |
| BspANI | GGCC | 1 cut(s) | 109 |
| BspCNI | CTCAG | 3 cut(s) | 195, 289, 309 |
| BspDI | ATCGAT | 1 cut(s) | 120 |
| BspEI | TCCGGA | 1 cut(s) | 52 |
| BspHI | TCATGA | 1 cut(s) | 259 |
| BspLI | GGNNCC | 2 cut(s) | 51, 174 |
| BspOI | GCTAGC | 1 cut(s) | 188 |
| BspPI | GGATC | 3 cut(s) | 44, 57, 274 |
| BspT107I | GGYRCC | 1 cut(s) | 172 |
| BspTI | CTTAAG | 1 cut(s) | 400 |
| BspTNI | GGTCTC | 1 cut(s) | 210 |
| BsrDI | GCAATG | 1 cut(s) | 340 |
| BsrI | ACTGG | 2 cut(s) | 109, 383 |
| BssMI | GATC | 5 cut(s) | 49, 206, 262, 279, 301 |
| BssNAI | GTATAC | 1 cut(s) | 308 |
| Bst1107I | GTATAC | 1 cut(s) | 308 |
| Bst4CI | ACNGT | 2 cut(s) | 34, 317 |
| Bst6I | CTCTTC | 1 cut(s) | 208 |
| BstAFI | CTTAAG | 1 cut(s) | 400 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 344 |
| BstC8I | GCNNGC | 1 cut(s) | 186 |
| BstDEI | CTNAG | 3 cut(s) | 203, 276, 296 |
| BstF5I | GGATG | 1 cut(s) | 150 |
| BstKTI | GATC | 5 cut(s) | 52, 209, 265, 282, 304 |
| BstMAI | GTCTC | 1 cut(s) | 210 |
| BstMBI | GATC | 5 cut(s) | 49, 206, 262, 279, 301 |
| BstMCI | CGRYCG | 1 cut(s) | 304 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 344, 411 |
| BstNSI | RCATGY | 1 cut(s) | 236 |
| BstSLI | GKGCMC | 1 cut(s) | 93 |
| BstV2I | GAAGAC | 1 cut(s) | 243 |
| BstX2I | RGATCY | 3 cut(s) | 49, 206, 279 |
| BstYI | RGATCY | 3 cut(s) | 49, 206, 279 |
| BstZ17I | GTATAC | 1 cut(s) | 308 |
| Bsu15I | ATCGAT | 1 cut(s) | 120 |
| BsuRI | GGCC | 1 cut(s) | 109 |
| BsuTUI | ATCGAT | 1 cut(s) | 120 |
| BtsCI | GGATG | 1 cut(s) | 150 |
| BtsIMutI | CAGTG | 1 cut(s) | 327 |
| Cac8I | GCNNGC | 1 cut(s) | 186 |
| CciI | TCATGA | 1 cut(s) | 259 |
| Cfr13I | GGNCC | 1 cut(s) | 107 |
| ClaI | ATCGAT | 1 cut(s) | 120 |
| Csp6I | GTAC | 1 cut(s) | 173 |
| CviAII | CATG | 3 cut(s) | 167, 233, 260 |
| CviJI | RGCY | 8 cut(s) | 5, 109, 146, 184, 188, 292, 357, 414 |
| CviKI_1 | RGCY | 8 cut(s) | 5, 109, 146, 184, 188, 292, 357, 414 |
| CviQI | GTAC | 1 cut(s) | 173 |
| DdeI | CTNAG | 3 cut(s) | 203, 276, 296 |
| DpnI | GATC | 5 cut(s) | 51, 208, 264, 281, 303 |
| DpnII | GATC | 5 cut(s) | 49, 206, 262, 279, 301 |
| Eam1104I | CTCTTC | 1 cut(s) | 208 |
| EarI | CTCTTC | 1 cut(s) | 208 |
| Eco31I | GGTCTC | 1 cut(s) | 210 |
| Eco32I | GATATC | 1 cut(s) | 397 |
| Eco57I | CTGAAG | 2 cut(s) | 126, 266 |
| EcoRI | GAATTC | 1 cut(s) | 371 |
| EcoRV | GATATC | 1 cut(s) | 397 |
| EcoT22I | ATGCAT | 1 cut(s) | 340 |
| FaeI | CATG | 3 cut(s) | 170, 236, 263 |
| FaiI | YATR | 6 cut(s) | 135, 168, 191, 234, 261, 308 |
| FaqI | GGGAC | 2 cut(s) | 43, 110 |
| FatI | CATG | 3 cut(s) | 166, 232, 259 |
| FbaI | TGATCA | 1 cut(s) | 262 |
| FblI | GTMKAC | 1 cut(s) | 307 |
| FokI | GGATG | 1 cut(s) | 137 |
| FspBI | CTAG | 2 cut(s) | 185, 415 |
| GsuI | CTGGAG | 1 cut(s) | 140 |
| HaeIII | GGCC | 1 cut(s) | 109 |
| HapII | CCGG | 1 cut(s) | 53 |
| Hin1II | CATG | 3 cut(s) | 170, 236, 263 |
| HincII | GTYRAC | 1 cut(s) | 102 |
| HindII | GTYRAC | 1 cut(s) | 102 |
| HindIII | AAGCTT | 1 cut(s) | 290 |
| HpaII | CCGG | 1 cut(s) | 53 |
| HphI | GGTGA | 2 cut(s) | 28, 170 |
| Hpy166II | GTNNAC | 3 cut(s) | 91, 102, 308 |
| Hpy188I | TCNGA | 2 cut(s) | 267, 279 |
| Hpy188III | TCNNGA | 4 cut(s) | 22, 53, 210, 260 |
| Hpy8I | GTNNAC | 3 cut(s) | 91, 102, 308 |
| HpyAV | CCTTC | 1 cut(s) | 150 |
| HpyCH4III | ACNGT | 2 cut(s) | 34, 317 |
| HpyCH4V | TGCA | 4 cut(s) | 85, 91, 338, 347 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 344, 411 |
| HpyF3I | CTNAG | 3 cut(s) | 203, 276, 296 |
| Hsp92II | CATG | 3 cut(s) | 170, 236, 263 |
| Kpn2I | TCCGGA | 1 cut(s) | 52 |
| KpnI | GGTACC | 1 cut(s) | 176 |
| Ksp22I | TGATCA | 1 cut(s) | 262 |
| Kzo9I | GATC | 5 cut(s) | 49, 206, 262, 279, 301 |
| LmnI | GCTCC | 1 cut(s) | 330 |
| LpnPI | CCDG | 7 cut(s) | 66, 90, 170, 194, 195, 364, 391 |
| LweI | GCATC | 1 cut(s) | 265 |
| MaeI | CTAG | 2 cut(s) | 185, 415 |
| MaeIII | GTNAC | 1 cut(s) | 34 |
| MalI | GATC | 5 cut(s) | 51, 208, 264, 281, 303 |
| MboI | GATC | 5 cut(s) | 49, 206, 262, 279, 301 |
| MboII | GAAGA | 4 cut(s) | 34, 225, 243, 404 |
| MflI | RGATCY | 3 cut(s) | 49, 206, 279 |
| MhlI | GDGCHC | 1 cut(s) | 93 |
| MluCI | AATT | 1 cut(s) | 371 |
| MnlI | CCTC | 7 cut(s) | 40, 49, 120, 138, 164, 186, 285 |
| Mph1103I | ATGCAT | 1 cut(s) | 340 |
| MroI | TCCGGA | 1 cut(s) | 52 |
| MseI | TTAA | 2 cut(s) | 12, 401 |
| MspCI | CTTAAG | 1 cut(s) | 400 |
| MspI | CCGG | 1 cut(s) | 53 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 344, 411 |
| NdeII | GATC | 5 cut(s) | 49, 206, 262, 279, 301 |
| NheI | GCTAGC | 1 cut(s) | 184 |
| NlaIII | CATG | 3 cut(s) | 170, 236, 263 |
| NlaIV | GGNNCC | 2 cut(s) | 51, 174 |
| NmuCI | GTSAC | 1 cut(s) | 34 |
| NsiI | ATGCAT | 1 cut(s) | 340 |
| NspI | RCATGY | 1 cut(s) | 236 |
| PagI | TCATGA | 1 cut(s) | 259 |
| Ple19I | CGATCG | 1 cut(s) | 304 |
| PspN4I | GGNNCC | 2 cut(s) | 51, 174 |
| PspPI | GGNCC | 1 cut(s) | 107 |
| PsuI | RGATCY | 3 cut(s) | 49, 206, 279 |
| PvuI | CGATCG | 1 cut(s) | 304 |
| RsaI | GTAC | 1 cut(s) | 174 |
| RsaNI | GTAC | 1 cut(s) | 173 |
| SaqAI | TTAA | 2 cut(s) | 12, 401 |
| Sau3AI | GATC | 5 cut(s) | 49, 206, 262, 279, 301 |
| Sau96I | GGNCC | 1 cut(s) | 107 |
| SduI | GDGCHC | 1 cut(s) | 93 |
| SetI | ASST | 6 cut(s) | 96, 148, 178, 186, 190, 294 |
| SfaNI | GCATC | 1 cut(s) | 265 |
| SmlI | CTYRAG | 2 cut(s) | 111, 400 |
| SmoI | CTYRAG | 2 cut(s) | 111, 400 |
| Sse9I | AATT | 1 cut(s) | 371 |
| SspMI | CTAG | 2 cut(s) | 185, 415 |
| TaaI | ACNGT | 2 cut(s) | 34, 317 |
| TaqI | TCGA | 3 cut(s) | 45, 120, 196 |
| TasI | AATT | 1 cut(s) | 371 |
| Tru1I | TTAA | 2 cut(s) | 12, 401 |
| Tru9I | TTAA | 2 cut(s) | 12, 401 |
| TscAI | CASTG | 1 cut(s) | 327 |
| TseFI | GTSAC | 1 cut(s) | 34 |
| Tsp45I | GTSAC | 1 cut(s) | 34 |
| TspRI | CASTG | 1 cut(s) | 327 |
| Vha464I | CTTAAG | 1 cut(s) | 400 |
| VneI | GTGCAC | 1 cut(s) | 89 |
| XapI | RAATTY | 1 cut(s) | 371 |
| XceI | RCATGY | 1 cut(s) | 236 |
| XmiI | GTMKAC | 1 cut(s) | 307 |
| XspI | CTAG | 2 cut(s) | 185, 415 |
| Zsp2I | ATGCAT | 1 cut(s) | 340 |