pycom420g00130

spliceosomal complex assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0000179
Physical Location & Seq
Forward (+)
124487 .. 124939
453 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom420g00130.1

Sequence Viewer

Length: 453 bp
ATGGCTGCAGGTGAATCTTTCAGTCTTGCTATCCCGATCTTGGCCAACATTTATAACGGCTTGAGTGTTGTTTCGAACTCGGCGAACACTGAAGATCGTGCTGCGGTTCTTCCCTACCACTATGTGTATGGTTGGTTGGGTGAGTACTTCGGCACTCATTTTTTTTCTTCAACTCTAGACAAGTCGGGGCCTTTGATGACGAAGTATTCTGGCGTGCTTTCTTCGAAGAGCCTCAATGATTCCCAGGCGCAAGCACTGTTTAGAAGTTGTGAAGGCTTGAAGATGGACCATCTGGCGAGAGTTGGCTTGGTGCGGCGAGGCATCATGGATGATTCGCATCTTCACTTCTCGGACTTATCTTACCTTATAAGTCTTCGCTCGGGGTATGTTTCTTTGCGGCAAGAAGATCGATGCATTGTTCAACCGTATAGTCCCCACCGTTTCAGCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve zf-CCHC

Protein Analysis

151

Amino Acids

16.73

Weight (kDa)

7.83

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000179)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18561 FvH4_1g29681 FvH4_2g15190 FvH4_3g22800 FvH4_3g22801 FvH4_3g26700 FvH4_4g15162 FvH4_4g15163 FvH4_4g15164 FvH4_4g22273 FvH4_7g02320 FvH4_7g03670 FvH4_7g03671
malus_domestica MD07G1097100.v1.1 MD11G1208600.v1.1 MD15G1303300.v1.1
prunus_persica Prupe.2G070800_v2.0.a1
pyrus_communis pycom02g13430 pycom04g06950 pycom04g09420 pycom04g09610 pycom06g05740 pycom07g00010 pycom07g06720 pycom07g09960 pycom07g10670 pycom07g10680 pycom07g10920 pycom07g27810 pycom08g12110 pycom08g13640 pycom09g03570 pycom09g13470 pycom09g13510 pycom10g01180 pycom10g02180 pycom10g06840 pycom12424g00130 pycom12g03090 pycom12g09570 pycom12g09580 pycom12g09590 pycom1310g00020 pycom13g25030 pycom13g25310 pycom13g25700 pycom13g26160 pycom13g27790 pycom13g28780 pycom13g28790 pycom1683g00070 pycom16g19330 pycom16g21210 pycom16g21710 pycom16g23400 pycom16g24730 pycom17g17550 pycom17g27670 pycom420g00110 pycom420g00130 pycom420g00610 pycom520g00600 pycom675g00050
rosa_chinensis RchiOBHm_Chr1g0316731
rosa_laevigata RLG00000030467
rosa_multiflora Rmu_co8112106.1_g000001 Rmu_co8301911.1_g000001 Rmu_sc0000161.1_g000042 Rmu_sc0000161.1_g000043 Rmu_sc0000173.1_g000035 Rmu_sc0000173.1_g000036 Rmu_sc0000480.1_g000024 Rmu_sc0000670.1_g000020 Rmu_sc0000670.1_g000021 Rmu_sc0000962.1_g000043 Rmu_sc0000962.1_g000044 Rmu_sc0001025.1_g000004 Rmu_sc0001075.1_g000026 Rmu_sc0001075.1_g000027 Rmu_sc0001167.1_g000050 Rmu_sc0001227.1_g000043 Rmu_sc0001346.1_g000006 Rmu_sc0001438.1_g000034 Rmu_sc0001946.1_g000002 Rmu_sc0001981.1_g000005 Rmu_sc0002106.1_g000005 Rmu_sc0002187.1_g000004 Rmu_sc0002187.1_g000005 Rmu_sc0002187.1_g000018 Rmu_sc0002539.1_g000097 Rmu_sc0003008.1_g000050 Rmu_sc0003008.1_g000051 Rmu_sc0003069.1_g000033 Rmu_sc0003069.1_g000034 Rmu_sc0003412.1_g000016 Rmu_sc0003553.1_g000005 Rmu_sc0003553.1_g000006 Rmu_sc0003641.1_g000025 Rmu_sc0003652.1_g000006 Rmu_sc0003687.1_g000019 Rmu_sc0003687.1_g000020 Rmu_sc0004234.1_g000013 Rmu_sc0004249.1_g000005 Rmu_sc0005044.1_g000027 Rmu_sc0005060.1_g000012 Rmu_sc0005816.1_g000005 Rmu_sc0006175.1_g000006 Rmu_sc0006175.1_g000007 Rmu_sc0006695.1_g000046 Rmu_sc0006725.1_g000014 Rmu_sc0006746.1_g000015 Rmu_sc0006746.1_g000016 Rmu_sc0006912.1_g000008 Rmu_sc0007355.1_g000004 Rmu_sc0007705.1_g000001 Rmu_sc0007903.1_g000003 Rmu_sc0007953.1_g000001 Rmu_sc0008630.1_g000001 Rmu_sc0008923.1_g000018 Rmu_sc0008923.1_g000019 Rmu_sc0009186.1_g000003 Rmu_sc0010184.1_g000007 Rmu_sc0010243.1_g000001 Rmu_sc0011242.1_g000004 Rmu_sc0012849.1_g000001 Rmu_sc0012849.1_g000002 Rmu_sc0012883.1_g000001 Rmu_sc0013028.1_g000015 Rmu_sc0015042.1_g000003 Rmu_sc0016730.1_g000002 Rmu_sc0017702.1_g000002 Rmu_sc0017791.1_g000007 Rmu_sc0017874.1_g000005 Rmu_sc0025334.1_g000001 Rmu_sc0025334.1_g000002 Rmu_sc0028789.1_g000001 Rmu_sc0030538.1_g000001 Rmu_sc0033137.1_g000001 Rmu_ssc0000183.1_g000013 Rmu_ssc0000259.1_g000061 Rmu_ssc0000259.1_g000062 Rmu_ssc0000259.1_g000063
rosa_roxburghii Rroxscaffold_173G00435030 Rroxscaffold_173G00435040 Rroxscaffold_1G00033030 Rroxscaffold_2G00093360 Rroxscaffold_2G00113080 Rroxscaffold_2G00146180 Rroxscaffold_3G00227920 Rroxscaffold_3G00251750 Rroxscaffold_4G00328240 Rroxscaffold_5G00333920 Rroxscaffold_5G00338240 Rroxscaffold_5G00342700 Rroxscaffold_6G00412810 Rroxscaffold_6G00423130 Rroxscaffold_7G00175190
rosa_rugosa Rorug01G0029200 Rorug01G0029300 Rorug01G0029400 Rorug01G0029400
rosa_samantha Rh1AG040900 Rh1BG038100 Rh1CG042000 Rh1DG027900
rosa_wichuraiana Rw0G019970 Rw1G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 54, 368
Acc36I ACCTGC 1 cut(s) 438
AciI CCGC 3 cut(s) 104, 313, 397
AcoI YGGCCR 1 cut(s) 42
AcuI CTGAAG 1 cut(s) 111
AdeI CACNNNGTG 1 cut(s) 124
AfaI GTAC 1 cut(s) 146
AfiI CCNNNNNNNGG 1 cut(s) 40
AgsI TTSAA 3 cut(s) 171, 280, 422
AjnI CCWGG 1 cut(s) 243
Ama87I CYCGRG 1 cut(s) 379
AoxI GGCC 2 cut(s) 42, 188
ApeKI GCWGC 2 cut(s) 5, 101
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 2 cut(s) 188, 286
AsuHPI GGTGA 2 cut(s) 23, 152
AsuII TTCGAA 2 cut(s) 74, 224
AvaI CYCGRG 1 cut(s) 379
AvaII GGWCC 1 cut(s) 286
BalI TGGCCA 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 365
BbvI GCAGC 1 cut(s) 88
BccI CCATC 2 cut(s) 277, 297
BceAI ACGGC 1 cut(s) 73
BcgI CGANNNNNNTGC 2 cut(s) 389, 423
BciT130I CCWGG 1 cut(s) 245
BfaI CTAG 1 cut(s) 176
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 1 cut(s) 438
BisI GCNGC 4 cut(s) 6, 102, 314, 398
BlsI GCNGC 4 cut(s) 7, 103, 315, 399
BmcAI AGTACT 1 cut(s) 146
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 1 cut(s) 286
BmeT110I CYCGRG 1 cut(s) 379
BmgT120I GGNCC 2 cut(s) 188, 286
BmiI GGNNCC 1 cut(s) 189
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 3 cut(s) 330, 346, 401
BpiI GAAGAC 1 cut(s) 365
Bpu14I TTCGAA 2 cut(s) 74, 224
BpuEI CTTGAG 1 cut(s) 82
Bsa29I ATCGAT 1 cut(s) 409
BsaBI GATNNNNATC 1 cut(s) 336
BsaJI CCNNGG 1 cut(s) 243
Bsc4I CCNNNNNNNGG 1 cut(s) 40
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 1 cut(s) 245
BseCI ATCGAT 1 cut(s) 409
BseDI CCNNGG 1 cut(s) 243
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 1 cut(s) 40
BseXI GCAGC 1 cut(s) 88
BshFI GGCC 2 cut(s) 44, 190
BshVI ATCGAT 1 cut(s) 409
BsiHKCI CYCGRG 1 cut(s) 379
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 417
BsnI GGCC 2 cut(s) 44, 190
BsoBI CYCGRG 1 cut(s) 379
Bsp119I TTCGAA 2 cut(s) 74, 224
Bsp143I GATC 3 cut(s) 36, 94, 406
BspACI CCGC 3 cut(s) 104, 313, 397
BspANI GGCC 2 cut(s) 44, 190
BspDI ATCGAT 1 cut(s) 409
BspLI GGNNCC 1 cut(s) 189
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 438
BspQI GCTCTTC 1 cut(s) 221
BspT104I TTCGAA 2 cut(s) 74, 224
BssECI CCNNGG 1 cut(s) 243
BssMI GATC 3 cut(s) 36, 94, 406
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 3 cut(s) 258, 426, 440
Bst6I CTCTTC 1 cut(s) 221
BstBI TTCGAA 2 cut(s) 74, 224
BstC8I GCNNGC 2 cut(s) 215, 252
BstF5I GGATG 1 cut(s) 334
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 3 cut(s) 39, 97, 409
BstMBI GATC 3 cut(s) 36, 94, 406
BstNI CCWGG 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 243
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 1 cut(s) 88
BstV2I GAAGAC 1 cut(s) 365
Bsu15I ATCGAT 1 cut(s) 409
BsuRI GGCC 2 cut(s) 44, 190
BsuTUI ATCGAT 1 cut(s) 409
BtsCI GGATG 1 cut(s) 334
BtsIMutI CAGTG 2 cut(s) 87, 254
BveI ACCTGC 1 cut(s) 438
Cac8I GCNNGC 2 cut(s) 215, 252
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 2 cut(s) 188, 286
ClaI ATCGAT 1 cut(s) 409
Csp6I GTAC 1 cut(s) 145
CviAII CATG 1 cut(s) 325
CviJI RGCY 7 cut(s) 5, 44, 60, 190, 231, 276, 306
CviKI_1 RGCY 7 cut(s) 5, 44, 60, 190, 231, 276, 306
CviQI GTAC 1 cut(s) 145
DpnI GATC 3 cut(s) 38, 96, 408
DpnII GATC 3 cut(s) 36, 94, 406
DraIII CACNNNGTG 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 111
Eco88I CYCGRG 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 243
EcoT22I ATGCAT 1 cut(s) 416
FaeI CATG 1 cut(s) 328
FaiI YATR 7 cut(s) 54, 123, 129, 326, 368, 387, 429
FaqI GGGAC 1 cut(s) 417
FatI CATG 1 cut(s) 324
Fnu4HI GCNGC 4 cut(s) 6, 102, 314, 398
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 4 cut(s) 6, 102, 314, 398
FspBI CTAG 1 cut(s) 176
GlaI GCGC 1 cut(s) 249
GluI GCNGC 4 cut(s) 6, 102, 314, 398
HaeIII GGCC 2 cut(s) 44, 190
HhaI GCGC 1 cut(s) 250
Hin1II CATG 1 cut(s) 328
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HinfI GANTC 3 cut(s) 14, 239, 332
HphI GGTGA 2 cut(s) 23, 152
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 2 cut(s) 34, 176
HpyAV CCTTC 1 cut(s) 266
HpyCH4III ACNGT 3 cut(s) 258, 426, 440
HpyCH4V TGCA 2 cut(s) 8, 414
Hsp92II CATG 1 cut(s) 328
HspAI GCGC 1 cut(s) 248
Kzo9I GATC 3 cut(s) 36, 94, 406
LguI GCTCTTC 1 cut(s) 221
LpnPI CCDG 5 cut(s) 195, 230, 257, 278, 433
Lsp1109I GCAGC 1 cut(s) 88
LweI GCATC 3 cut(s) 330, 346, 401
MaeI CTAG 1 cut(s) 176
MalI GATC 3 cut(s) 38, 96, 408
MboI GATC 3 cut(s) 36, 94, 406
MboII GAAGA 9 cut(s) 101, 104, 159, 213, 238, 292, 332, 365, 416
MlsI TGGCCA 1 cut(s) 44
MluNI TGGCCA 1 cut(s) 44
MnlI CCTC 2 cut(s) 242, 311
Mox20I TGGCCA 1 cut(s) 44
Mph1103I ATGCAT 1 cut(s) 416
MscI TGGCCA 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 44
MspR9I CCNGG 1 cut(s) 245
MvaI CCWGG 1 cut(s) 245
NdeII GATC 3 cut(s) 36, 94, 406
NlaIII CATG 1 cut(s) 328
NlaIV GGNNCC 1 cut(s) 189
NmeAIII GCCGAG 1 cut(s) 59
NsiI ATGCAT 1 cut(s) 416
NspV TTCGAA 2 cut(s) 74, 224
PciSI GCTCTTC 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PfeI GAWTC 3 cut(s) 14, 239, 332
PkrI GCNGC 4 cut(s) 7, 103, 315, 399
PsiI TTATAA 2 cut(s) 54, 368
Psp6I CCWGG 1 cut(s) 243
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 1 cut(s) 189
PspPI GGNCC 2 cut(s) 188, 286
PstI CTGCAG 1 cut(s) 10
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
SapI GCTCTTC 1 cut(s) 221
SatI GCNGC 4 cut(s) 6, 102, 314, 398
Sau3AI GATC 3 cut(s) 36, 94, 406
Sau96I GGNCC 2 cut(s) 188, 286
ScaI AGTACT 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 245
SetI ASST 3 cut(s) 13, 366, 452
SfaNI GCATC 3 cut(s) 330, 346, 401
SfcI CTRYAG 1 cut(s) 6
SfuI TTCGAA 2 cut(s) 74, 224
SinI GGWCC 1 cut(s) 286
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
SsiI CCGC 3 cut(s) 104, 313, 397
SspMI CTAG 1 cut(s) 176
StyD4I CCNGG 1 cut(s) 243
TaaI ACNGT 3 cut(s) 258, 426, 440
TaqI TCGA 3 cut(s) 74, 224, 409
TatI WGTACW 1 cut(s) 144
TauI GCSGC 2 cut(s) 316, 400
TfiI GAWTC 3 cut(s) 14, 239, 332
TscAI CASTG 2 cut(s) 94, 261
TseI GCWGC 2 cut(s) 5, 101
TspRI CASTG 2 cut(s) 94, 261
VpaK11BI GGWCC 1 cut(s) 286
XbaI TCTAGA 1 cut(s) 175
XcmI CCANNNNNNNNNTGG 1 cut(s) 125
XspI CTAG 1 cut(s) 176
ZrmI AGTACT 1 cut(s) 146
Zsp2I ATGCAT 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.