pycom520g00600

spliceosomal complex assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0000179
Physical Location & Seq
Forward (+)
491117 .. 494284
3168 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom520g00600.10

Sequence Viewer

Length: 2322 bp
ATGTGCCAGGCAAATATGCATTGGGAATCTTGTACCCGTGCATACACAAATGCCTCTATAACCCTGCCAACCAAGGATGAGTTCAAGAGTAATCTAGTGACATGTGCCTACGTAGGTTGGTGGTCAAAGGTACACCATGAAGAATTGGGGATGACAAGTGGCACCAATTCTTCTCGTTCACACCATGATGCGCCAAGAGAAGTGAATGTAGCTCATTTGCAAGTCGGACCTGTAGTTTTAACTCTTCAACGCCCGTGTTTGTCTTCTCAAAATGCAGATGCTTCTGAAGAGGTGGGTGATGGAGTTGATTCGCACGAAGATAAACTTATTTTGCGGCAGCATCAACAAGTTTTGAACAAACGACCACTTGAAGGTAAAGTGTTTAGACCTCTTCAGTTTGACGGCTGTATGTTACTTGAGAAAGATGTATGTATTTCCTTCTTTCTTGTGATTTCTTCTACTTTCCTTGGTTTAGCTTTTGTTTTTAACACCTTTCTTTGGGCGTTCATTCCTTTACAAAGATGCTATTTGGAGGGAATCACTACAAATAAGGAGATTGGGGATCCGCTCGGTGAAGAGCTTGTTCCAAATTTGCCCAGTTGTCCAAGTTTACTGTGTGTAGGTGAAGGTATGCAAGAGCCCATCATGCGTCTAACACCTTTGCCAGCTAGCTCTGAGATCTCTCTTAGAAGCCTGAACATTAGTGGCATTAACAAGTGTCATGTTGAGAAGAGGATTGTGAAGTGCCCATTGGAGGACCTTGCGTTGCTCAAGGAAGACTTATCGAAGCTTGTTTCTATGATTGACAACCTTAACGTTGATTCCTCGCTCTTGAGAGTCAAGATTGCCAAAATTATGGCCGCCTCGACTGAGTATTCTTCCTTTCATGCTATTTCCTCAAAGAAGTTGAGTCCAGACGTTAGAGCTCAACAACTTGCAGTAATAAACTTATCAATTCAAGGTACATGGATTTCTTTGGCTTCGGTCCAAGCACGTTTAGAAGCGTTTCCGCATGAGCGAGAGCAGTTGGAAATTGAACATCAAGAGGAGGTTTCTTACCTACGGGCCATGGAAATGCTCACTTTGTCCCCTACTGATGTAGAGACTTTGAAGACTTCGGAAGGCTTTAGTGTCTTCCATTTTTTGTGCTTGAAGCCTTCTTTGTTTTTGCTCATTTTTTTTCACATTCTTGAATTTTTCTTTATTCTTCAAAGTGTTTGTGTTTTTCTTGATCGTGTGACTGTATTTGAGGTTTTGAAGCTTCAAGTTGCCTACGTACCCTCGGTTAAGGATGGATCAAGTCATGACGTAGTTCAAAGGAGTGATGGATTGTTTTTTTTTTTTTTTTTTTTTTTTGAAGATTTTGGTGGTGATCTTGATGATAATTTTAATGGTGATTTTGGTTTTTTGGTGCTGTGTTGCAGTTTTAGCTTGTGCAGGCACGAAACTTTGTGCCATGTGCAGTTTAGGCTCGTGCAGGCGAGGAGCTTTGTGCCGTTTATAGTTTGGGCTCATGCAGGTGAGGAGCTTTGTGCTGTGTGTAGTTTAGGCTCGTGTAAGCGAGGAGCTTTGCTTGTTTGCTGCCGCCATGATGAAGTAGACGCCGTTGGTGTAGACCTCATGTATTACATAAGTTGTGATGATGGCTTTTGTAATCCCAAGGCAAGATCTCCAGTTTGGAAAGACCTTGGGCGGACCTTCTTGTTAAATGCTGATGAGTCCATATTATACTATATATTTGACCCTAATCTTAGTTATAATTTATTGGTTATTTTGTCGACTTCCTCTGGAGAAAAAAGCGATCAAACGGATGAATTTTATGACTGTTCAAAATCCCAGATTTTTCTACCCGGCCGTTTGACCGTGGCGATGAAATTAAGGCCCAGCGCGCAGCTTTCCCAGATTGACATTTTGAGGAAGATTCCTTTAAGGATTTGTAGGGGACGTCTTCAGCTTTCAAAAGAGACATTTTGGGACCAAATCGGAGTTGATTTGGTCGGTCAAAAGTTTGATTTTCTGAGAAGTCCGAATTTTAGTTTAAATAGGAAACCTTTTATTTTCTGTTTTATTATTTTATTATTTTTTAGTAGTATAACTAAGAGGGTTTTTGAAGTTTATTTTCTAGGTGTTTTCTATGTTTTTTTTTTCTATTTCAATAAAATTCCTTTTGTTTTATGGTTGTTCGTAACTAATTTCCATTTGCTAGGGCGATGCCTTGAATTATACATGCATTACTTTGAATTATTAAGCATTATAAGTAATAGGTTGCAATTCGGTCGGAATACCACCGGAGAATTGAGTATTGCTTCTTGTGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve zf-CCHC

Protein Analysis

774

Amino Acids

87.97

Weight (kDa)

5.81

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000179)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18561 FvH4_1g29681 FvH4_2g15190 FvH4_3g22800 FvH4_3g22801 FvH4_3g26700 FvH4_4g15162 FvH4_4g15163 FvH4_4g15164 FvH4_4g22273 FvH4_7g02320 FvH4_7g03670 FvH4_7g03671
malus_domestica MD07G1097100.v1.1 MD11G1208600.v1.1 MD15G1303300.v1.1
prunus_persica Prupe.2G070800_v2.0.a1
pyrus_communis pycom02g13430 pycom04g06950 pycom04g09420 pycom04g09610 pycom06g05740 pycom07g00010 pycom07g06720 pycom07g09960 pycom07g10670 pycom07g10680 pycom07g10920 pycom07g27810 pycom08g12110 pycom08g13640 pycom09g03570 pycom09g13470 pycom09g13510 pycom10g01180 pycom10g02180 pycom10g06840 pycom12424g00130 pycom12g03090 pycom12g09570 pycom12g09580 pycom12g09590 pycom1310g00020 pycom13g25030 pycom13g25310 pycom13g25700 pycom13g26160 pycom13g27790 pycom13g28780 pycom13g28790 pycom1683g00070 pycom16g19330 pycom16g21210 pycom16g21710 pycom16g23400 pycom16g24730 pycom17g17550 pycom17g27670 pycom420g00110 pycom420g00130 pycom420g00610 pycom520g00600 pycom675g00050
rosa_chinensis RchiOBHm_Chr1g0316731
rosa_laevigata RLG00000030467
rosa_multiflora Rmu_co8112106.1_g000001 Rmu_co8301911.1_g000001 Rmu_sc0000161.1_g000042 Rmu_sc0000161.1_g000043 Rmu_sc0000173.1_g000035 Rmu_sc0000173.1_g000036 Rmu_sc0000480.1_g000024 Rmu_sc0000670.1_g000020 Rmu_sc0000670.1_g000021 Rmu_sc0000962.1_g000043 Rmu_sc0000962.1_g000044 Rmu_sc0001025.1_g000004 Rmu_sc0001075.1_g000026 Rmu_sc0001075.1_g000027 Rmu_sc0001167.1_g000050 Rmu_sc0001227.1_g000043 Rmu_sc0001346.1_g000006 Rmu_sc0001438.1_g000034 Rmu_sc0001946.1_g000002 Rmu_sc0001981.1_g000005 Rmu_sc0002106.1_g000005 Rmu_sc0002187.1_g000004 Rmu_sc0002187.1_g000005 Rmu_sc0002187.1_g000018 Rmu_sc0002539.1_g000097 Rmu_sc0003008.1_g000050 Rmu_sc0003008.1_g000051 Rmu_sc0003069.1_g000033 Rmu_sc0003069.1_g000034 Rmu_sc0003412.1_g000016 Rmu_sc0003553.1_g000005 Rmu_sc0003553.1_g000006 Rmu_sc0003641.1_g000025 Rmu_sc0003652.1_g000006 Rmu_sc0003687.1_g000019 Rmu_sc0003687.1_g000020 Rmu_sc0004234.1_g000013 Rmu_sc0004249.1_g000005 Rmu_sc0005044.1_g000027 Rmu_sc0005060.1_g000012 Rmu_sc0005816.1_g000005 Rmu_sc0006175.1_g000006 Rmu_sc0006175.1_g000007 Rmu_sc0006695.1_g000046 Rmu_sc0006725.1_g000014 Rmu_sc0006746.1_g000015 Rmu_sc0006746.1_g000016 Rmu_sc0006912.1_g000008 Rmu_sc0007355.1_g000004 Rmu_sc0007705.1_g000001 Rmu_sc0007903.1_g000003 Rmu_sc0007953.1_g000001 Rmu_sc0008630.1_g000001 Rmu_sc0008923.1_g000018 Rmu_sc0008923.1_g000019 Rmu_sc0009186.1_g000003 Rmu_sc0010184.1_g000007 Rmu_sc0010243.1_g000001 Rmu_sc0011242.1_g000004 Rmu_sc0012849.1_g000001 Rmu_sc0012849.1_g000002 Rmu_sc0012883.1_g000001 Rmu_sc0013028.1_g000015 Rmu_sc0015042.1_g000003 Rmu_sc0016730.1_g000002 Rmu_sc0017702.1_g000002 Rmu_sc0017791.1_g000007 Rmu_sc0017874.1_g000005 Rmu_sc0025334.1_g000001 Rmu_sc0025334.1_g000002 Rmu_sc0028789.1_g000001 Rmu_sc0030538.1_g000001 Rmu_sc0033137.1_g000001 Rmu_ssc0000183.1_g000013 Rmu_ssc0000259.1_g000061 Rmu_ssc0000259.1_g000062 Rmu_ssc0000259.1_g000063
rosa_roxburghii Rroxscaffold_173G00435030 Rroxscaffold_173G00435040 Rroxscaffold_1G00033030 Rroxscaffold_2G00093360 Rroxscaffold_2G00113080 Rroxscaffold_2G00146180 Rroxscaffold_3G00227920 Rroxscaffold_3G00251750 Rroxscaffold_4G00328240 Rroxscaffold_5G00333920 Rroxscaffold_5G00338240 Rroxscaffold_5G00342700 Rroxscaffold_6G00412810 Rroxscaffold_6G00423130 Rroxscaffold_7G00175190
rosa_rugosa Rorug01G0029200 Rorug01G0029300 Rorug01G0029400 Rorug01G0029400
rosa_samantha Rh1AG040900 Rh1BG038100 Rh1CG042000 Rh1DG027900
rosa_wichuraiana Rw0G019970 Rw1G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1758, 2255
AarI CACCTGC 1 cut(s) 1508
AatII GACGTC 1 cut(s) 1948
Acc36I ACCTGC 1 cut(s) 1508
AccB1I GGYRCC 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 568
AccI GTMKAC 3 cut(s) 1599, 1614, 1778
AccII CGCG 1 cut(s) 1889
AciI CCGC 6 cut(s) 334, 566, 861, 1010, 1585, 1693
AclI AACGTT 1 cut(s) 816
AclWI GGATC 3 cut(s) 557, 570, 1303
AcoI YGGCCR 2 cut(s) 858, 1852
AcsI RAATTY 5 cut(s) 589, 1193, 1814, 2029, 2160
AcuI CTGAAG 3 cut(s) 306, 377, 1934
AcyI GRCGYC 2 cut(s) 1602, 1945
AfaI GTAC 4 cut(s) 34, 132, 964, 1278
AfiI CCNNNNNNNGG 3 cut(s) 371, 498, 754
AflIII ACRYGT 1 cut(s) 101
AjnI CCWGG 1 cut(s) 6
AjuI GAANNNNNNNTTGG 4 cut(s) 65, 97, 734, 766
Alw21I GWGCWC 1 cut(s) 928
Alw26I GTCTC 2 cut(s) 1097, 1958
AlwI GGATC 3 cut(s) 557, 570, 1303
AoxI GGCC 4 cut(s) 858, 1065, 1852, 1880
ApeKI GCWGC 3 cut(s) 337, 1581, 1891
ApoI RAATTY 5 cut(s) 589, 1193, 1814, 2029, 2160
Asp700I GAANNNNTTC 1 cut(s) 1005
AspLEI GCGC 3 cut(s) 193, 1889, 1891
AspS9I GGNCC 7 cut(s) 227, 757, 985, 1065, 1695, 1881, 1975
AsuC2I CCSGG 1 cut(s) 1851
AsuHPI GGTGA 6 cut(s) 308, 584, 635, 1382, 1406, 1532
AsuNHI GCTAGC 1 cut(s) 668
AvaII GGWCC 5 cut(s) 227, 757, 985, 1695, 1975
BaeGI GKGCMC 1 cut(s) 749
BamHI GGATCC 1 cut(s) 562
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 3 cut(s) 642, 928, 1513
BauI CACGAG 2 cut(s) 1472, 1552
BbsI GAAGAC 5 cut(s) 255, 783, 1118, 1126, 1940
Bbv12I GWGCWC 1 cut(s) 928
BbvI GCAGC 3 cut(s) 349, 1568, 1903
BccI CCATC 5 cut(s) 293, 650, 1286, 1319, 1637
BceAI ACGGC 4 cut(s) 418, 1480, 1589, 1839
BcgI CGANNNNNNTGC 2 cut(s) 2258, 2292
BciT130I CCWGG 1 cut(s) 8
BcnI CCSGG 1 cut(s) 1851
BcoDI GTCTC 2 cut(s) 1097, 1958
BfaI CTAG 4 cut(s) 95, 669, 2123, 2204
BfmI CTRYAG 1 cut(s) 231
BfuAI ACCTGC 1 cut(s) 1508
BglII AGATCT 2 cut(s) 678, 1667
BisI GCNGC 6 cut(s) 335, 338, 861, 1582, 1585, 1892
BlsI GCNGC 6 cut(s) 336, 339, 862, 1583, 1586, 1893
Bme1390I CCNGG 2 cut(s) 8, 1851
Bme18I GGWCC 5 cut(s) 227, 757, 985, 1695, 1975
BmgT120I GGNCC 7 cut(s) 227, 757, 985, 1065, 1695, 1881, 1975
BmiI GGNNCC 3 cut(s) 163, 564, 1976
BmrFI CCNGG 2 cut(s) 8, 1851
BmrI ACTGGG 1 cut(s) 591
BmsI GCATC 5 cut(s) 178, 268, 349, 512, 2201
BmtI GCTAGC 1 cut(s) 672
BmuI ACTGGG 1 cut(s) 591
BpiI GAAGAC 5 cut(s) 255, 783, 1118, 1126, 1940
BpmI CTGGAG 2 cut(s) 1656, 1809
BpuEI CTTGAG 3 cut(s) 437, 755, 853
BpuMI CCSGG 1 cut(s) 1851
BsaAI YACGTR 2 cut(s) 112, 1276
BsaHI GRCGYC 2 cut(s) 1602, 1945
BsaJI CCNNGG 7 cut(s) 72, 466, 1068, 1281, 1659, 1687, 1863
BsaWI WCCGGW 1 cut(s) 2288
Bsc4I CCNNNNNNNGG 3 cut(s) 371, 498, 754
Bse1I ACTGG 2 cut(s) 597, 1673
BseBI CCWGG 1 cut(s) 8
BseDI CCNNGG 7 cut(s) 72, 466, 1068, 1281, 1659, 1687, 1863
BseGI GGATG 4 cut(s) 82, 156, 1297, 1816
BseLI CCNNNNNNNGG 3 cut(s) 371, 498, 754
BseMII CTCAG 3 cut(s) 666, 861, 2009
BseNI ACTGG 2 cut(s) 597, 1673
BsePI GCGCGC 1 cut(s) 1887
BseRI GAGGAG 4 cut(s) 1061, 1498, 1538, 1578
BseSI GKGCMC 1 cut(s) 749
BseX3I CGGCCG 1 cut(s) 1852
BseXI GCAGC 3 cut(s) 349, 1568, 1903
BseYI CCCAGC 1 cut(s) 1883
BsgI GTGCAG 3 cut(s) 1456, 1481, 1496
Bsh1236I CGCG 1 cut(s) 1889
Bsh1285I CGRYCG 2 cut(s) 1855, 2278
BshFI GGCC 4 cut(s) 860, 1067, 1854, 1882
BshNI GGYRCC 1 cut(s) 161
BsiEI CGRYCG 2 cut(s) 1855, 2278
BsiHKAI GWGCWC 1 cut(s) 928
BsiSI CCGG 2 cut(s) 1851, 2289
BslFI GGGAC 3 cut(s) 1072, 1956, 1988
BslI CCNNNNNNNGG 3 cut(s) 371, 498, 754
BsmAI GTCTC 2 cut(s) 1097, 1958
BsmFI GGGAC 3 cut(s) 1072, 1956, 1988
BsnI GGCC 4 cut(s) 860, 1067, 1854, 1882
Bsp1286I GDGCHC 4 cut(s) 642, 749, 928, 1513
Bsp143I GATC 7 cut(s) 562, 678, 1231, 1295, 1372, 1667, 1801
Bsp19I CCATGG 1 cut(s) 1068
BspACI CCGC 6 cut(s) 334, 566, 861, 1010, 1585, 1693
BspANI GGCC 4 cut(s) 860, 1067, 1854, 1882
BspCNI CTCAG 3 cut(s) 667, 862, 2010
BspFNI CGCG 1 cut(s) 1889
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 3 cut(s) 163, 564, 1976
BspMI ACCTGC 1 cut(s) 1508
BspOI GCTAGC 1 cut(s) 672
BspPI GGATC 3 cut(s) 557, 570, 1303
BspQI GCTCTTC 1 cut(s) 570
BspT107I GGYRCC 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 568
BsrI ACTGG 2 cut(s) 597, 1673
BssECI CCNNGG 7 cut(s) 72, 466, 1068, 1281, 1659, 1687, 1863
BssHII GCGCGC 1 cut(s) 1887
BssMI GATC 7 cut(s) 562, 678, 1231, 1295, 1372, 1667, 1801
BssNI GRCGYC 2 cut(s) 1602, 1945
BssSI CACGAG 2 cut(s) 1472, 1552
BssT1I CCWWGG 5 cut(s) 72, 466, 1068, 1659, 1687
Bst2BI CACGAG 2 cut(s) 1472, 1552
Bst2UI CCWGG 1 cut(s) 8
Bst4CI ACNGT 4 cut(s) 615, 1243, 1826, 1864
Bst6I CTCTTC 5 cut(s) 249, 282, 396, 570, 725
BstACI GRCGYC 2 cut(s) 1602, 1945
BstBAI YACGTR 2 cut(s) 112, 1276
BstC8I GCNNGC 5 cut(s) 666, 670, 1439, 1479, 1889
BstDEI CTNAG 6 cut(s) 675, 686, 870, 1751, 2018, 2097
BstDSI CCRYGG 2 cut(s) 1068, 1863
BstF5I GGATG 4 cut(s) 82, 156, 1297, 1816
BstFNI CGCG 1 cut(s) 1889
BstHHI GCGC 3 cut(s) 193, 1889, 1891
BstKTI GATC 7 cut(s) 565, 681, 1234, 1298, 1375, 1670, 1804
BstMAI GTCTC 2 cut(s) 1097, 1958
BstMBI GATC 7 cut(s) 562, 678, 1231, 1295, 1372, 1667, 1801
BstMCI CGRYCG 2 cut(s) 1855, 2278
BstMWI GCNNNNNNNGC 4 cut(s) 646, 1428, 1468, 1888
BstNI CCWGG 1 cut(s) 8
BstNSI RCATGY 2 cut(s) 105, 2230
BstSCI CCNGG 2 cut(s) 6, 1849
BstSFI CTRYAG 1 cut(s) 231
BstSLI GKGCMC 1 cut(s) 749
BstSNI TACGTA 2 cut(s) 112, 1276
BstUI CGCG 1 cut(s) 1889
BstV1I GCAGC 3 cut(s) 349, 1568, 1903
BstV2I GAAGAC 5 cut(s) 255, 783, 1118, 1126, 1940
BstX2I RGATCY 3 cut(s) 562, 678, 1667
BstXI CCANNNNNNTGG 1 cut(s) 856
BstYI RGATCY 3 cut(s) 562, 678, 1667
BstZI CGGCCG 1 cut(s) 1852
BsuRI GGCC 4 cut(s) 860, 1067, 1854, 1882
BtgI CCRYGG 2 cut(s) 1068, 1863
BtgZI GCGATG 2 cut(s) 1883, 2224
BtsCI GGATG 4 cut(s) 82, 156, 1297, 1816
BveI ACCTGC 1 cut(s) 1508
Cac8I GCNNGC 5 cut(s) 666, 670, 1439, 1479, 1889
CciI TCATGA 1 cut(s) 1303
CfoI GCGC 3 cut(s) 193, 1889, 1891
Cfr13I GGNCC 7 cut(s) 227, 757, 985, 1065, 1695, 1881, 1975
CseI GACGC 2 cut(s) 638, 1610
Csp6I GTAC 4 cut(s) 33, 131, 963, 1277
CviQI GTAC 4 cut(s) 33, 131, 963, 1277
DdeI CTNAG 6 cut(s) 675, 686, 870, 1751, 2018, 2097
DpnI GATC 7 cut(s) 564, 680, 1233, 1297, 1374, 1669, 1803
DpnII GATC 7 cut(s) 562, 678, 1231, 1295, 1372, 1667, 1801
DraI TTTAAA 1 cut(s) 2040
EaeI YGGCCR 2 cut(s) 858, 1852
EagI CGGCCG 1 cut(s) 1852
Eam1104I CTCTTC 5 cut(s) 249, 282, 396, 570, 725
EarI CTCTTC 5 cut(s) 249, 282, 396, 570, 725
EciI GGCGGA 1 cut(s) 1708
Ecl136II GAGCTC 1 cut(s) 926
EclXI CGGCCG 1 cut(s) 1852
Eco105I TACGTA 2 cut(s) 112, 1276
Eco130I CCWWGG 5 cut(s) 72, 466, 1068, 1659, 1687
Eco24I GRGCYC 3 cut(s) 642, 928, 1513
Eco47I GGWCC 5 cut(s) 227, 757, 985, 1695, 1975
Eco52I CGGCCG 1 cut(s) 1852
Eco53kI GAGCTC 1 cut(s) 926
Eco57I CTGAAG 3 cut(s) 306, 377, 1934
EcoICRI GAGCTC 1 cut(s) 926
EcoO109I RGGNCCY 1 cut(s) 757
EcoRII CCWGG 1 cut(s) 6
EcoT14I CCWWGG 5 cut(s) 72, 466, 1068, 1659, 1687
EcoT22I ATGCAT 2 cut(s) 21, 2232
EcoT38I GRGCYC 3 cut(s) 642, 928, 1513
ErhI CCWWGG 5 cut(s) 72, 466, 1068, 1659, 1687
FalI AAGNNNNNCTT 4 cut(s) 309, 341, 764, 796
FaqI GGGAC 3 cut(s) 1072, 1956, 1988
FblI GTMKAC 3 cut(s) 1599, 1614, 1778
Fnu4HI GCNGC 6 cut(s) 335, 338, 861, 1582, 1585, 1892
FokI GGATG 4 cut(s) 89, 163, 1304, 1823
FriOI GRGCYC 3 cut(s) 642, 928, 1513
Fsp4HI GCNGC 6 cut(s) 335, 338, 861, 1582, 1585, 1892
FspBI CTAG 4 cut(s) 95, 669, 2123, 2204
GlaI GCGC 3 cut(s) 192, 1888, 1890
GluI GCNGC 6 cut(s) 335, 338, 861, 1582, 1585, 1892
GsaI CCCAGC 1 cut(s) 1887
GsuI CTGGAG 2 cut(s) 1656, 1809
HaeIII GGCC 4 cut(s) 860, 1067, 1854, 1882
HapII CCGG 2 cut(s) 1851, 2289
HgaI GACGC 2 cut(s) 638, 1610
HhaI GCGC 3 cut(s) 193, 1889, 1891
Hin1I GRCGYC 2 cut(s) 1602, 1945
Hin6I GCGC 3 cut(s) 191, 1887, 1889
HinP1I GCGC 3 cut(s) 191, 1887, 1889
HincII GTYRAC 1 cut(s) 1779
HindII GTYRAC 1 cut(s) 1779
HindIII AAGCTT 2 cut(s) 788, 1259
HinfI GANTC 8 cut(s) 26, 308, 537, 821, 837, 910, 1718, 1921
HpaII CCGG 2 cut(s) 1851, 2289
HphI GGTGA 6 cut(s) 308, 584, 635, 1382, 1406, 1532
Hpy166II GTNNAC 6 cut(s) 133, 179, 611, 1600, 1615, 1779
Hpy188I TCNGA 8 cut(s) 227, 286, 676, 1120, 1985, 2019, 2028, 2280
Hpy8I GTNNAC 6 cut(s) 133, 179, 611, 1600, 1615, 1779
HpyAV CCTTC 6 cut(s) 365, 448, 620, 1115, 1167, 1708
HpyCH4III ACNGT 4 cut(s) 615, 1243, 1826, 1864
HpyCH4IV ACGT 7 cut(s) 111, 816, 918, 994, 1275, 1308, 1945
HpyF10VI GCNNNNNNNGC 4 cut(s) 646, 1428, 1468, 1888
HpyF3I CTNAG 6 cut(s) 675, 686, 870, 1751, 2018, 2097
HpySE526I ACGT 7 cut(s) 111, 816, 918, 994, 1275, 1308, 1945
Hsp92I GRCGYC 2 cut(s) 1602, 1945
HspAI GCGC 3 cut(s) 191, 1887, 1889
Kzo9I GATC 7 cut(s) 562, 678, 1231, 1295, 1372, 1667, 1801
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 3 cut(s) 1485, 1525, 1565
Lsp1109I GCAGC 3 cut(s) 349, 1568, 1903
LweI GCATC 5 cut(s) 178, 268, 349, 512, 2201
MaeI CTAG 4 cut(s) 95, 669, 2123, 2204
MaeII ACGT 7 cut(s) 111, 816, 918, 994, 1275, 1308, 1945
MaeIII GTNAC 4 cut(s) 97, 411, 1237, 2185
MalI GATC 7 cut(s) 564, 680, 1233, 1297, 1374, 1669, 1803
MbiI CCGCTC 1 cut(s) 568
MboI GATC 7 cut(s) 562, 678, 1231, 1295, 1372, 1667, 1801
MflI RGATCY 3 cut(s) 562, 678, 1667
MhlI GDGCHC 4 cut(s) 642, 749, 928, 1513
MlyI GAGTC 3 cut(s) 846, 919, 1727
MmeI TCCRAC 3 cut(s) 205, 1008, 2258
Mph1103I ATGCAT 2 cut(s) 21, 2232
MroXI GAANNNNTTC 1 cut(s) 1005
MslI CAYNNNNRTG 3 cut(s) 186, 1073, 1518
MspI CCGG 2 cut(s) 1851, 2289
MspR9I CCNGG 2 cut(s) 8, 1851
MvaI CCWGG 1 cut(s) 8
MvnI CGCG 1 cut(s) 1889
MwoI GCNNNNNNNGC 4 cut(s) 646, 1428, 1468, 1888
NciI CCSGG 1 cut(s) 1851
NcoI CCATGG 1 cut(s) 1068
NdeII GATC 7 cut(s) 562, 678, 1231, 1295, 1372, 1667, 1801
NheI GCTAGC 1 cut(s) 668
NlaIV GGNNCC 3 cut(s) 163, 564, 1976
NmuCI GTSAC 2 cut(s) 97, 1237
NsiI ATGCAT 2 cut(s) 21, 2232
NspI RCATGY 2 cut(s) 105, 2230
PagI TCATGA 1 cut(s) 1303
PaqCI CACCTGC 1 cut(s) 1508
PauI GCGCGC 1 cut(s) 1887
PciI ACATGT 1 cut(s) 101
PciSI GCTCTTC 1 cut(s) 570
PdmI GAANNNNTTC 1 cut(s) 1005
PfeI GAWTC 5 cut(s) 26, 308, 537, 821, 1921
PkrI GCNGC 6 cut(s) 336, 339, 862, 1583, 1586, 1893
PleI GAGTC 3 cut(s) 845, 918, 1726
PpsI GAGTC 3 cut(s) 845, 918, 1726
Ppu21I YACGTR 2 cut(s) 112, 1276
PpuMI RGGWCCY 1 cut(s) 757
PscI ACATGT 1 cut(s) 101
PsiI TTATAA 2 cut(s) 1758, 2255
Psp124BI GAGCTC 1 cut(s) 928
Psp1406I AACGTT 1 cut(s) 816
Psp5II RGGWCCY 1 cut(s) 757
Psp6I CCWGG 1 cut(s) 6
PspFI CCCAGC 1 cut(s) 1883
PspGI CCWGG 1 cut(s) 6
PspN4I GGNNCC 3 cut(s) 163, 564, 1976
PspPI GGNCC 7 cut(s) 227, 757, 985, 1065, 1695, 1881, 1975
PspPPI RGGWCCY 1 cut(s) 757
PsuI RGATCY 3 cut(s) 562, 678, 1667
PteI GCGCGC 1 cut(s) 1887
RsaI GTAC 4 cut(s) 34, 132, 964, 1278
RsaNI GTAC 4 cut(s) 33, 131, 963, 1277
RseI CAYNNNNRTG 3 cut(s) 186, 1073, 1518
SacI GAGCTC 1 cut(s) 928
SalI GTCGAC 1 cut(s) 1777
SapI GCTCTTC 1 cut(s) 570
SatI GCNGC 6 cut(s) 335, 338, 861, 1582, 1585, 1892
Sau3AI GATC 7 cut(s) 562, 678, 1231, 1295, 1372, 1667, 1801
Sau96I GGNCC 7 cut(s) 227, 757, 985, 1065, 1695, 1881, 1975
SchI GAGTC 3 cut(s) 846, 919, 1727
ScrFI CCNGG 2 cut(s) 8, 1851
SduI GDGCHC 4 cut(s) 642, 749, 928, 1513
SfaNI GCATC 5 cut(s) 178, 268, 349, 512, 2201
SfcI CTRYAG 1 cut(s) 231
SinI GGWCC 5 cut(s) 227, 757, 985, 1695, 1975
SmiMI CAYNNNNRTG 3 cut(s) 186, 1073, 1518
SmlI CTYRAG 3 cut(s) 416, 770, 832
SmoI CTYRAG 3 cut(s) 416, 770, 832
SnaBI TACGTA 2 cut(s) 112, 1276
SsiI CCGC 6 cut(s) 334, 566, 861, 1010, 1585, 1693
SspMI CTAG 4 cut(s) 95, 669, 2123, 2204
SstI GAGCTC 1 cut(s) 928
StyD4I CCNGG 2 cut(s) 6, 1849
StyI CCWWGG 5 cut(s) 72, 466, 1068, 1659, 1687
TaaI ACNGT 4 cut(s) 615, 1243, 1826, 1864
TaiI ACGT 7 cut(s) 114, 819, 921, 997, 1278, 1311, 1948
TaqI TCGA 3 cut(s) 785, 866, 1778
TaqII GACCGA 3 cut(s) 973, 1988, 2264
TauI GCSGC 3 cut(s) 337, 863, 1587
TfiI GAWTC 5 cut(s) 26, 308, 537, 821, 1921
TseFI GTSAC 2 cut(s) 97, 1237
TseI GCWGC 3 cut(s) 337, 1581, 1891
Tsp45I GTSAC 2 cut(s) 97, 1237
TspDTI ATGAA 6 cut(s) 153, 496, 875, 1608, 1827, 1886
TspGWI ACGGA 1 cut(s) 1823
VpaK11BI GGWCC 5 cut(s) 227, 757, 985, 1695, 1975
XapI RAATTY 5 cut(s) 589, 1193, 1814, 2029, 2160
XceI RCATGY 2 cut(s) 105, 2230
XmiI GTMKAC 3 cut(s) 1599, 1614, 1778
XmnI GAANNNNTTC 1 cut(s) 1005
XspI CTAG 4 cut(s) 95, 669, 2123, 2204
ZraI GACGTC 1 cut(s) 1946
Zsp2I ATGCAT 2 cut(s) 21, 2232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.