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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfiI | CCNNNNNNNGG | 1 cut(s) | 144 |
| BmsI | GCATC | 1 cut(s) | 27 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 144 |
| BseGI | GGATG | 1 cut(s) | 42 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 144 |
| BsiSI | CCGG | 1 cut(s) | 138 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 144 |
| BsmI | GAATGC | 1 cut(s) | 115 |
| BstF5I | GGATG | 1 cut(s) | 42 |
| BtsCI | GGATG | 1 cut(s) | 42 |
| FaiI | YATR | 1 cut(s) | 104 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 14, 46 |
| FokI | GGATG | 1 cut(s) | 49 |
| HapII | CCGG | 1 cut(s) | 138 |
| HincII | GTYRAC | 2 cut(s) | 27, 67 |
| HindII | GTYRAC | 2 cut(s) | 27, 67 |
| HpaII | CCGG | 1 cut(s) | 138 |
| Hpy166II | GTNNAC | 3 cut(s) | 27, 67, 89 |
| Hpy188III | TCNNGA | 1 cut(s) | 55 |
| Hpy8I | GTNNAC | 3 cut(s) | 27, 67, 89 |
| LpnPI | CCDG | 1 cut(s) | 151 |
| LweI | GCATC | 1 cut(s) | 27 |
| MluCI | AATT | 1 cut(s) | 93 |
| MnlI | CCTC | 2 cut(s) | 91, 130 |
| MspI | CCGG | 1 cut(s) | 138 |
| Mva1269I | GAATGC | 1 cut(s) | 115 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 60 |
| PctI | GAATGC | 1 cut(s) | 115 |
| SetI | ASST | 1 cut(s) | 32 |
| SfaNI | GCATC | 1 cut(s) | 27 |
| SgeI | CNNG | 7 cut(s) | 17, 29, 34, 67, 82, 150, 154 |
| Sse9I | AATT | 1 cut(s) | 93 |
| TasI | AATT | 1 cut(s) | 93 |
| TspDTI | ATGAA | 1 cut(s) | 125 |
| TspGWI | ACGGA | 1 cut(s) | 49 |