pycom13g25700

NatA auxiliary

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000179
Physical Location & Seq
Forward (+)
22395329 .. 22399136
3808 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g25700.23

Sequence Viewer

Length: 2706 bp
ATGCTCCTATCATGTAGTTGTTTTTTCATTGCAGTCGCTGCTTTTTCATTGTCATCTCTGCTTTTTCATTGCCATCACTATTTTTTTTCATTATCGACACTGCTTTTCATTATCGTTGCTGCTTCCATTGTCATTGCAGCTTTTCATTGTCGTTGCAGCTTTCCACTGTCATCGCTGTTTTTTTTCCTCTGCTGCCTACATGATGTGACACTACCAACTGCTAAAAATGTGTGCTTAGGGCCTTTAACTGTCTTTATTTGTGCCCTGCGAGGCAAATTTTTTATTTCCCTTTTGTCGTTACTATTTATATTTCTTTTTTTCTTTTATACAGTTATGGCGTTCCTCAATAGCTACAAGAATAGCACTATCACCATTCTTACAAAGGATGGGGAATCTCAAGACAAGGGAACCACACTACCACTTCATGCACTTTTGACTGGTCCTAAAACGCTTATCCGTTCGGTAACGGACTTTGGGCGGTCAAGATCTAATGATAGGGAATGTGCGTCCAATGTTTTGATTTTAAATTCTTCACATCATGTTCGTGACAGCTTATCCGCCTCTTTGGAACTCCTTGGTGATCGTATCCGTAATGGGACCTGGCTTGAAGAAGTTCTAAGTCGGTCTAGAGTCGTTTTACGAATATGGGGCTTTACGACATTGTATTTGCCTCTTTATTTAGCCATGATAGCCATCCTCTTGTTGTTTGTGGGAAATTTTATGACGAAGTTGGTCCTTATGTTGAAGAGCTTTCTTATTGAAATAGTCAAGGCCTGCCTGCTAGTTGTCGGAATTTGTTATTTGCATATCATAAGCCTTCTCAAGAAGTCAAAGGTAAATCTGGGGTCAAGTTTAGGAAATCTCTCAAAAAGAATGGAAGAAACAAAACTACAAAGCCGAAAAGGCGACTATGATCTGACCGATGTTATTAGGTCAGCTAGACGACATACTTCCGCAGAACAAAAAGTCTTTGATGAATTATGTATAGCTTTTGAGCATTTGTTTTTCCAAAAAAATGATGTCAATTTCATCCACCCAGGGGTGTTCAAAGTTGCTAGTAAAATGGCCGTGGGTGAGCTCTTCAACGTTACTATCCTGGTGTTGGCCAATATCTATGACGGTTTGAATGAGATTTCTAATTCAGCAAATCCTGAAGATTGTGTAGCTGTCATTCCTTTCCATTATGTGTATGTTTGGTTGGGCAAATATTTCGGCACCCACTTCTCATCGCAAACTTCCGACAAGGCCAGCCTTGATGATTCACAAACGTGTGCTTTGTTTAGAGATTGTGAAAACTTTCGAATGGATTGCCTAGCCAAAGTAGGGTCAGGGCGCCAAAAACTTATTGACGACTTGTGCAACTTCGTATATTACTTTGAGATAGGAGAATCAGCATGCACAAAATTAGTGAAGACTTTGGAACAACGTAAAGTGTCAAGTTTAGATAGAGATAGGGAAGTAAACACGAGATGTACATGGTTCACATCTAATGGAAAACCCTTTTCGCTCTGCTTAGCCCTATCCCTCTCTAGGGGTATTTTAGTGATAGGTTCTATAGGAACTGAACGATCCTATTTGGTCAAATACCTAGCGATAAACTTCTATGTCCCTTTCATTACAGTATTTCTGAACAAGTTATTGGCTAACAAGCCTAACGGTTTTCTTGTTGATGATAGTGACGACCGTGACTTTGATATGGAGCTAATGCTTCTAACTATGATGAATGCACTAACTATGGATATGATGCTGAAAATAGACCGATTTTATATCACATTTCAATTCGAATTAGCAAAAGCAATGTCTCCTTGCATAATATGGATTCACCCTAAGTCTGGCAACGTCCACGGAGTGGAGAAGTTCTCATTAATAGTTCTGATGACCAGTTTAAGTACAATAATGACATTTGGGATTAATTCTTCTATCCGTGTCGATGTGGGACTCTATGAGCTTTATTATAACGTTGACATGTGTCGTGCTGTGATTGGCCTCCTGGTTGGAGGGAAACTCTTGTGCTTCGGTAAGGGTGCTTCGGCAAGAACCCCTCAGTGGGTTCTGGAAGGTATGGCTTTAATCGGTTACTCCCCTAAGTTCCCAAGTGAGGAAAGTTTCTCAGTTGGAGACGTGCAAAATCCAATAGCATATAGTGGGAGTCCCTTAAGTCTCCGGGCAAGGAAAGTTAACAAGGCTTCAACCTTTACTTTTCTAGGTGGTAGCCCAAAACTCCTTTCCCATATGCCCCAGCCCACTTTCATTGTTGGCAGCCTTTCTCTCTTTTTAATTATTTTTGTGTGTAGGTTTATTGTAGATTTTTGTGATTTTGCTTCACACTATCTTGATCAAGAGTGTGTGAAGCTTTCTACATGTTGTAGTGTTGAAGCTTTTTTGGTTAAAGCTTTCTTTGTAGATGAAGATTTTAGTTTGTATGTTGAAGCTTGTAAGTTGAACTTGTATGTTGAAAGACACCATAATTTGATTTTGCTTCACACTATCTTGATCAAGAATGTCTTTGTAGGTGTAGTTTTTAGGATGAAGCTTTGTAGCTTTGTAGGTGAAGCTTTTAGGTTGAAGCTTTGTAGCTTTGTAGATGAAGCTTTTTGGTTTAATCTTTGTAGCGTTGGGCACCATAAATTGATTTTGCTTCACACCATCTTGATCAAGAGCGACTTAGTTAGCTTGTTTTCTTACATTTATGTTGTTAGTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NARP1 TPR_11 TPR_2

Protein Analysis

902

Amino Acids

101.47

Weight (kDa)

8.44

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000179)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18561 FvH4_1g29681 FvH4_2g15190 FvH4_3g22800 FvH4_3g22801 FvH4_3g26700 FvH4_4g15162 FvH4_4g15163 FvH4_4g15164 FvH4_4g22273 FvH4_7g02320 FvH4_7g03670 FvH4_7g03671
malus_domestica MD07G1097100.v1.1 MD11G1208600.v1.1 MD15G1303300.v1.1
prunus_persica Prupe.2G070800_v2.0.a1
pyrus_communis pycom02g13430 pycom04g06950 pycom04g09420 pycom04g09610 pycom06g05740 pycom07g00010 pycom07g06720 pycom07g09960 pycom07g10670 pycom07g10680 pycom07g10920 pycom07g27810 pycom08g12110 pycom08g13640 pycom09g03570 pycom09g13470 pycom09g13510 pycom10g01180 pycom10g02180 pycom10g06840 pycom12424g00130 pycom12g03090 pycom12g09570 pycom12g09580 pycom12g09590 pycom1310g00020 pycom13g25030 pycom13g25310 pycom13g25700 pycom13g26160 pycom13g27790 pycom13g28780 pycom13g28790 pycom1683g00070 pycom16g19330 pycom16g21210 pycom16g21710 pycom16g23400 pycom16g24730 pycom17g17550 pycom17g27670 pycom420g00110 pycom420g00130 pycom420g00610 pycom520g00600 pycom675g00050
rosa_chinensis RchiOBHm_Chr1g0316731
rosa_laevigata RLG00000030467
rosa_multiflora Rmu_co8112106.1_g000001 Rmu_co8301911.1_g000001 Rmu_sc0000161.1_g000042 Rmu_sc0000161.1_g000043 Rmu_sc0000173.1_g000035 Rmu_sc0000173.1_g000036 Rmu_sc0000480.1_g000024 Rmu_sc0000670.1_g000020 Rmu_sc0000670.1_g000021 Rmu_sc0000962.1_g000043 Rmu_sc0000962.1_g000044 Rmu_sc0001025.1_g000004 Rmu_sc0001075.1_g000026 Rmu_sc0001075.1_g000027 Rmu_sc0001167.1_g000050 Rmu_sc0001227.1_g000043 Rmu_sc0001346.1_g000006 Rmu_sc0001438.1_g000034 Rmu_sc0001946.1_g000002 Rmu_sc0001981.1_g000005 Rmu_sc0002106.1_g000005 Rmu_sc0002187.1_g000004 Rmu_sc0002187.1_g000005 Rmu_sc0002187.1_g000018 Rmu_sc0002539.1_g000097 Rmu_sc0003008.1_g000050 Rmu_sc0003008.1_g000051 Rmu_sc0003069.1_g000033 Rmu_sc0003069.1_g000034 Rmu_sc0003412.1_g000016 Rmu_sc0003553.1_g000005 Rmu_sc0003553.1_g000006 Rmu_sc0003641.1_g000025 Rmu_sc0003652.1_g000006 Rmu_sc0003687.1_g000019 Rmu_sc0003687.1_g000020 Rmu_sc0004234.1_g000013 Rmu_sc0004249.1_g000005 Rmu_sc0005044.1_g000027 Rmu_sc0005060.1_g000012 Rmu_sc0005816.1_g000005 Rmu_sc0006175.1_g000006 Rmu_sc0006175.1_g000007 Rmu_sc0006695.1_g000046 Rmu_sc0006725.1_g000014 Rmu_sc0006746.1_g000015 Rmu_sc0006746.1_g000016 Rmu_sc0006912.1_g000008 Rmu_sc0007355.1_g000004 Rmu_sc0007705.1_g000001 Rmu_sc0007903.1_g000003 Rmu_sc0007953.1_g000001 Rmu_sc0008630.1_g000001 Rmu_sc0008923.1_g000018 Rmu_sc0008923.1_g000019 Rmu_sc0009186.1_g000003 Rmu_sc0010184.1_g000007 Rmu_sc0010243.1_g000001 Rmu_sc0011242.1_g000004 Rmu_sc0012849.1_g000001 Rmu_sc0012849.1_g000002 Rmu_sc0012883.1_g000001 Rmu_sc0013028.1_g000015 Rmu_sc0015042.1_g000003 Rmu_sc0016730.1_g000002 Rmu_sc0017702.1_g000002 Rmu_sc0017791.1_g000007 Rmu_sc0017874.1_g000005 Rmu_sc0025334.1_g000001 Rmu_sc0025334.1_g000002 Rmu_sc0028789.1_g000001 Rmu_sc0030538.1_g000001 Rmu_sc0033137.1_g000001 Rmu_ssc0000183.1_g000013 Rmu_ssc0000259.1_g000061 Rmu_ssc0000259.1_g000062 Rmu_ssc0000259.1_g000063
rosa_roxburghii Rroxscaffold_173G00435030 Rroxscaffold_173G00435040 Rroxscaffold_1G00033030 Rroxscaffold_2G00093360 Rroxscaffold_2G00113080 Rroxscaffold_2G00146180 Rroxscaffold_3G00227920 Rroxscaffold_3G00251750 Rroxscaffold_4G00328240 Rroxscaffold_5G00333920 Rroxscaffold_5G00338240 Rroxscaffold_5G00342700 Rroxscaffold_6G00412810 Rroxscaffold_6G00423130 Rroxscaffold_7G00175190
rosa_rugosa Rorug01G0029200 Rorug01G0029300 Rorug01G0029400 Rorug01G0029400
rosa_samantha Rh1AG040900 Rh1BG038100 Rh1CG042000 Rh1DG027900
rosa_wichuraiana Rw0G019970 Rw1G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1956
AccB1I GGYRCC 3 cut(s) 1214, 1332, 2619
AccB7I CCANNNNNTGG 1 cut(s) 1849
AciI CCGC 3 cut(s) 478, 558, 954
AclI AACGTT 2 cut(s) 1086, 1959
AclWI GGATC 1 cut(s) 1563
AcoI YGGCCR 2 cut(s) 1065, 1104
AcsI RAATTY 4 cut(s) 275, 526, 715, 792
AcuI CTGAAG 1 cut(s) 1173
AcyI GRCGYC 1 cut(s) 1333
AdeI CACNNNGTG 1 cut(s) 1849
AfaI GTAC 2 cut(s) 1474, 1891
AflII CTTAAG 1 cut(s) 2155
AflIII ACRYGT 3 cut(s) 1268, 1965, 2360
AjiI CACGTC 1 cut(s) 2122
AjnI CCWGG 4 cut(s) 599, 1036, 1095, 1989
AjuI GAANNNNNNNTTGG 4 cut(s) 1622, 1654, 2209, 2241
AleI CACNNNNGTG 1 cut(s) 1267
Alw21I GWGCWC 1 cut(s) 1080
Alw26I GTCTC 3 cut(s) 1806, 2112, 2165
AlwI GGATC 1 cut(s) 1563
AlwNI CAGNNNCTG 1 cut(s) 38
AoxI GGCC 6 cut(s) 239, 771, 1065, 1104, 1245, 1984
ApeKI GCWGC 6 cut(s) 38, 119, 137, 156, 192, 2259
ApoI RAATTY 4 cut(s) 275, 526, 715, 792
ArsI GACNNNNNNTTYG 6 cut(s) 649, 681, 1673, 1705, 1784, 1816
AseI ATTAAT 2 cut(s) 1865, 1911
Asp700I GAANNNNTTC 2 cut(s) 612, 1296
AspLEI GCGC 1 cut(s) 1335
AspS9I GGNCC 4 cut(s) 239, 440, 597, 733
AsuC2I CCSGG 1 cut(s) 2165
AsuHPI GGTGA 5 cut(s) 361, 590, 1085, 1814, 2561
AsuII TTCGAA 2 cut(s) 1300, 1782
AvaII GGWCC 3 cut(s) 440, 597, 733
BaeGI GKGCMC 2 cut(s) 265, 2622
BalI TGGCCA 1 cut(s) 1106
BanI GGYRCC 3 cut(s) 1214, 1332, 2619
BanII GRGCYC 1 cut(s) 1080
BarI GAAGNNNNNNTAC 2 cut(s) 2011, 2043
BauI CACGAG 1 cut(s) 1465
BbsI GAAGAC 1 cut(s) 1418
Bbv12I GWGCWC 1 cut(s) 1080
BbvI GCAGC 6 cut(s) 25, 106, 149, 168, 179, 2271
BccI CCATC 4 cut(s) 81, 380, 701, 2654
BceAI ACGGC 1 cut(s) 1052
BcgI CGANNNNNNTGC 4 cut(s) 1192, 1226, 1290, 1324
BciT130I CCWGG 4 cut(s) 601, 1038, 1097, 1991
BciVI GTATCC 1 cut(s) 596
BclI TGATCA 3 cut(s) 2335, 2493, 2652
BcnI CCSGG 1 cut(s) 2165
BcoDI GTCTC 3 cut(s) 1806, 2112, 2165
BfaI CTAG 8 cut(s) 627, 782, 939, 1056, 1313, 1530, 1589, 2204
BfmI CTRYAG 1 cut(s) 1554
BfoI RGCGCY 1 cut(s) 1336
BfrI CTTAAG 1 cut(s) 2155
BfuI GTATCC 1 cut(s) 596
BglI GCCNNNNNGGC 1 cut(s) 903
BglII AGATCT 1 cut(s) 485
BisI GCNGC 6 cut(s) 39, 120, 138, 157, 193, 2260
BlpI GCTNAGC 1 cut(s) 1513
BlsI GCNGC 6 cut(s) 40, 121, 139, 158, 194, 2261
Bme1390I CCNGG 5 cut(s) 601, 1038, 1097, 1991, 2165
Bme18I GGWCC 3 cut(s) 440, 597, 733
BmgBI CACGTC 1 cut(s) 2122
BmgT120I GGNCC 4 cut(s) 239, 440, 597, 733
BmiI GGNNCC 5 cut(s) 409, 598, 1216, 1334, 2621
BmrFI CCNGG 5 cut(s) 601, 1038, 1097, 1991, 2165
BmsI GCATC 1 cut(s) 1734
BoxI GACNNNNGTC 1 cut(s) 1968
BpiI GAAGAC 1 cut(s) 1418
BplI GAGNNNNNCTC 4 cut(s) 1844, 1876, 1989, 2021
Bpu10I CCTNAGC 1 cut(s) 235
Bpu1102I GCTNAGC 1 cut(s) 1513
Bpu14I TTCGAA 2 cut(s) 1300, 1782
BpuEI CTTGAG 2 cut(s) 381, 806
BpuMI CCSGG 1 cut(s) 2165
BsaBI GATNNNNATC 1 cut(s) 692
BsaHI GRCGYC 1 cut(s) 1333
BsaJI CCNNGG 5 cut(s) 574, 1036, 1037, 1068, 1843
Bse1I ACTGG 2 cut(s) 442, 1881
Bse3DI GCAATG 4 cut(s) 27, 67, 132, 1803
Bse8I GATNNNNATC 1 cut(s) 692
BseBI CCWGG 4 cut(s) 601, 1038, 1097, 1991
BseDI CCNNGG 5 cut(s) 574, 1036, 1037, 1068, 1843
BseGI GGATG 4 cut(s) 391, 693, 1029, 2532
BseJI GATNNNNATC 1 cut(s) 692
BseMI GCAATG 4 cut(s) 27, 67, 132, 1803
BseMII CTCAG 2 cut(s) 2057, 2124
BseNI ACTGG 2 cut(s) 442, 1881
BseSI GKGCMC 2 cut(s) 265, 2622
BseXI GCAGC 6 cut(s) 25, 106, 149, 168, 179, 2271
BseYI CCCAGC 1 cut(s) 2238
Bsh1285I CGRYCG 1 cut(s) 1684
BshFI GGCC 6 cut(s) 241, 773, 1067, 1106, 1247, 1986
BshNI GGYRCC 3 cut(s) 1214, 1332, 2619
BsiEI CGRYCG 1 cut(s) 1684
BsiHKAI GWGCWC 1 cut(s) 1080
BsiSI CCGG 1 cut(s) 2164
BslFI GGGAC 4 cut(s) 610, 1592, 1950, 2136
BsmAI GTCTC 3 cut(s) 1806, 2112, 2165
BsmBI CGTCTC 1 cut(s) 2112
BsmFI GGGAC 4 cut(s) 610, 1592, 1950, 2136
BsmI GAATGC 1 cut(s) 1729
BsnI GGCC 6 cut(s) 241, 773, 1067, 1106, 1247, 1986
Bsp119I TTCGAA 2 cut(s) 1300, 1782
Bsp1286I GDGCHC 3 cut(s) 265, 1080, 2622
Bsp1407I TGTACA 1 cut(s) 1472
Bsp143I GATC 7 cut(s) 485, 580, 913, 1568, 2335, 2493, 2652
Bsp1720I GCTNAGC 1 cut(s) 1513
BspACI CCGC 3 cut(s) 478, 558, 954
BspANI GGCC 6 cut(s) 241, 773, 1067, 1106, 1247, 1986
BspCNI CTCAG 2 cut(s) 2056, 2123
BspLI GGNNCC 5 cut(s) 409, 598, 1216, 1334, 2621
BspPI GGATC 1 cut(s) 1563
BspQI GCTCTTC 2 cut(s) 740, 1085
BspT104I TTCGAA 2 cut(s) 1300, 1782
BspT107I GGYRCC 3 cut(s) 1214, 1332, 2619
BspTI CTTAAG 1 cut(s) 2155
BsrDI GCAATG 4 cut(s) 27, 67, 132, 1803
BsrGI TGTACA 1 cut(s) 1472
BsrI ACTGG 2 cut(s) 442, 1881
BssECI CCNNGG 5 cut(s) 574, 1036, 1037, 1068, 1843
BssMI GATC 7 cut(s) 485, 580, 913, 1568, 2335, 2493, 2652
BssNI GRCGYC 1 cut(s) 1333
BssSI CACGAG 1 cut(s) 1465
BssT1I CCWWGG 1 cut(s) 574
Bst2BI CACGAG 1 cut(s) 1465
Bst2UI CCWGG 4 cut(s) 601, 1038, 1097, 1991
Bst4CI ACNGT 7 cut(s) 168, 250, 331, 1121, 1621, 1658, 1685
Bst6I CTCTTC 2 cut(s) 740, 1085
BstACI GRCGYC 1 cut(s) 1333
BstAFI CTTAAG 1 cut(s) 2155
BstAPI GCANNNNNTGC 1 cut(s) 38
BstAUI TGTACA 1 cut(s) 1472
BstBI TTCGAA 2 cut(s) 1300, 1782
BstC8I GCNNGC 4 cut(s) 775, 779, 1249, 1396
BstDEI CTNAG 8 cut(s) 235, 617, 1513, 1827, 2043, 2085, 2110, 2665
BstDSI CCRYGG 2 cut(s) 1068, 1843
BstF5I GGATG 4 cut(s) 391, 693, 1029, 2532
BstH2I RGCGCY 1 cut(s) 1336
BstHHI GCGC 1 cut(s) 1335
BstKTI GATC 7 cut(s) 488, 583, 916, 1571, 2338, 2496, 2655
BstMAI GTCTC 3 cut(s) 1806, 2112, 2165
BstMBI GATC 7 cut(s) 485, 580, 913, 1568, 2335, 2493, 2652
BstMCI CGRYCG 1 cut(s) 1684
BstMWI GCNNNNNNNGC 3 cut(s) 38, 689, 903
BstNI CCWGG 4 cut(s) 601, 1038, 1097, 1991
BstNSI RCATGY 3 cut(s) 1398, 1969, 2364
BstPAI GACNNNNGTC 1 cut(s) 1968
BstSCI CCNGG 5 cut(s) 599, 1036, 1095, 1989, 2163
BstSFI CTRYAG 1 cut(s) 1554
BstSLI GKGCMC 2 cut(s) 265, 2622
BstV1I GCAGC 6 cut(s) 25, 106, 149, 168, 179, 2271
BstV2I GAAGAC 1 cut(s) 1418
BstX2I RGATCY 1 cut(s) 485
BstYI RGATCY 1 cut(s) 485
BsuI GTATCC 1 cut(s) 596
BsuRI GGCC 6 cut(s) 241, 773, 1067, 1106, 1247, 1986
BtgI CCRYGG 2 cut(s) 1068, 1843
BtgZI GCGATG 2 cut(s) 156, 1212
BtrI CACGTC 1 cut(s) 2122
BtsCI GGATG 4 cut(s) 391, 693, 1029, 2532
BtsI GCAGTG 1 cut(s) 98
BtsIMutI CAGTG 3 cut(s) 98, 164, 2051
Cac8I GCNNGC 4 cut(s) 775, 779, 1249, 1396
CaiI CAGNNNCTG 1 cut(s) 38
CfoI GCGC 1 cut(s) 1335
Cfr13I GGNCC 4 cut(s) 239, 440, 597, 733
CseI GACGC 1 cut(s) 495
Csp6I GTAC 2 cut(s) 1473, 1890
CspCI CAANNNNNGTGG 2 cut(s) 2233, 2268
CviAII CATG 9 cut(s) 12, 200, 425, 539, 685, 1395, 1476, 1966, 2361
CviQI GTAC 2 cut(s) 1473, 1890
DdeI CTNAG 8 cut(s) 235, 617, 1513, 1827, 2043, 2085, 2110, 2665
DinI GGCGCC 1 cut(s) 1334
DpnI GATC 7 cut(s) 487, 582, 915, 1570, 2337, 2495, 2654
DpnII GATC 7 cut(s) 485, 580, 913, 1568, 2335, 2493, 2652
DraI TTTAAA 1 cut(s) 525
DraIII CACNNNGTG 1 cut(s) 1849
EaeI YGGCCR 2 cut(s) 1065, 1104
Eam1104I CTCTTC 2 cut(s) 740, 1085
EarI CTCTTC 2 cut(s) 740, 1085
EciI GGCGGA 1 cut(s) 547
Ecl136II GAGCTC 1 cut(s) 1078
Eco130I CCWWGG 1 cut(s) 574
Eco147I AGGCCT 1 cut(s) 773
Eco24I GRGCYC 1 cut(s) 1080
Eco47I GGWCC 3 cut(s) 440, 597, 733
Eco53kI GAGCTC 1 cut(s) 1078
Eco57I CTGAAG 1 cut(s) 1173
EcoICRI GAGCTC 1 cut(s) 1078
EcoO109I RGGNCCY 2 cut(s) 239, 597
EcoRII CCWGG 4 cut(s) 599, 1036, 1095, 1989
EcoT14I CCWWGG 1 cut(s) 574
EcoT38I GRGCYC 1 cut(s) 1080
EgeI GGCGCC 1 cut(s) 1334
EheI GGCGCC 1 cut(s) 1334
ErhI CCWWGG 1 cut(s) 574
Esp3I CGTCTC 1 cut(s) 2112
FaeI CATG 9 cut(s) 15, 203, 428, 542, 688, 1398, 1479, 1969, 2364
FalI AAGNNNNNCTT 6 cut(s) 2429, 2461, 2489, 2521, 2648, 2680
FaqI GGGAC 4 cut(s) 610, 1592, 1950, 2136
FatI CATG 9 cut(s) 11, 199, 424, 538, 684, 1394, 1475, 1965, 2360
FauNDI CATATG 1 cut(s) 2232
FbaI TGATCA 3 cut(s) 2335, 2493, 2652
Fnu4HI GCNGC 6 cut(s) 39, 120, 138, 157, 193, 2260
FokI GGATG 4 cut(s) 398, 680, 1016, 2539
FriOI GRGCYC 1 cut(s) 1080
Fsp4HI GCNGC 6 cut(s) 39, 120, 138, 157, 193, 2260
FspBI CTAG 8 cut(s) 627, 782, 939, 1056, 1313, 1530, 1589, 2204
GlaI GCGC 1 cut(s) 1334
GluI GCNGC 6 cut(s) 39, 120, 138, 157, 193, 2260
GsaI CCCAGC 1 cut(s) 2242
HaeII RGCGCY 1 cut(s) 1336
HaeIII GGCC 6 cut(s) 241, 773, 1067, 1106, 1247, 1986
HapII CCGG 1 cut(s) 2164
HgaI GACGC 1 cut(s) 495
HhaI GCGC 1 cut(s) 1335
Hin1I GRCGYC 1 cut(s) 1333
Hin1II CATG 9 cut(s) 15, 203, 428, 542, 688, 1398, 1479, 1969, 2364
Hin6I GCGC 1 cut(s) 1333
HinP1I GCGC 1 cut(s) 1333
HincII GTYRAC 2 cut(s) 1963, 2179
HindII GTYRAC 2 cut(s) 1963, 2179
HindIII AAGCTT 8 cut(s) 2351, 2376, 2391, 2430, 2531, 2553, 2567, 2589
HinfI GANTC 7 cut(s) 392, 630, 1259, 1388, 1819, 1938, 2149
HpaI GTTAAC 1 cut(s) 2179
HpaII CCGG 1 cut(s) 2164
HphI GGTGA 5 cut(s) 361, 590, 1085, 1814, 2561
Hpy166II GTNNAC 5 cut(s) 1462, 1482, 1843, 1963, 2179
Hpy188I TCNGA 5 cut(s) 791, 918, 1240, 1629, 1875
Hpy8I GTNNAC 5 cut(s) 1462, 1482, 1843, 1963, 2179
HpyAV CCTTC 2 cut(s) 827, 2051
HpyCH4III ACNGT 7 cut(s) 168, 250, 331, 1121, 1621, 1658, 1685
HpyCH4IV ACGT 6 cut(s) 1086, 1268, 1426, 1839, 1959, 2121
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 689, 903
HpyF3I CTNAG 8 cut(s) 235, 617, 1513, 1827, 2043, 2085, 2110, 2665
HpySE526I ACGT 6 cut(s) 1086, 1268, 1426, 1839, 1959, 2121
Hsp92I GRCGYC 1 cut(s) 1333
Hsp92II CATG 9 cut(s) 15, 203, 428, 542, 688, 1398, 1479, 1969, 2364
HspAI GCGC 1 cut(s) 1333
KasI GGCGCC 1 cut(s) 1332
Ksp22I TGATCA 3 cut(s) 2335, 2493, 2652
KspAI GTTAAC 1 cut(s) 2179
Kzo9I GATC 7 cut(s) 485, 580, 913, 1568, 2335, 2493, 2652
LguI GCTCTTC 2 cut(s) 740, 1085
LmnI GCTCC 2 cut(s) 9, 1699
Lsp1109I GCAGC 6 cut(s) 25, 106, 149, 168, 179, 2271
LweI GCATC 1 cut(s) 1734
MaeI CTAG 8 cut(s) 627, 782, 939, 1056, 1313, 1530, 1589, 2204
MaeII ACGT 6 cut(s) 1086, 1268, 1426, 1839, 1959, 2121
MaeIII GTNAC 8 cut(s) 205, 297, 463, 545, 1087, 1676, 1685, 2075
MalI GATC 7 cut(s) 487, 582, 915, 1570, 2337, 2495, 2654
MboI GATC 7 cut(s) 485, 580, 913, 1568, 2335, 2493, 2652
MboII GAAGA 9 cut(s) 522, 620, 757, 890, 1072, 1166, 1423, 1908, 2420
MflI RGATCY 1 cut(s) 485
MhlI GDGCHC 3 cut(s) 265, 1080, 2622
MlsI TGGCCA 1 cut(s) 1106
MluNI TGGCCA 1 cut(s) 1106
Mly113I GGCGCC 1 cut(s) 1333
MlyI GAGTC 3 cut(s) 639, 1932, 2158
MmeI TCCRAC 4 cut(s) 769, 1263, 1975, 2095
Mox20I TGGCCA 1 cut(s) 1106
MroXI GAANNNNTTC 2 cut(s) 612, 1296
MscI TGGCCA 1 cut(s) 1106
MslI CAYNNNNRTG 2 cut(s) 543, 1267
Msp20I TGGCCA 1 cut(s) 1106
MspCI CTTAAG 1 cut(s) 2155
MspI CCGG 1 cut(s) 2164
MspR9I CCNGG 5 cut(s) 601, 1038, 1097, 1991, 2165
Mva1269I GAATGC 1 cut(s) 1729
MvaI CCWGG 4 cut(s) 601, 1038, 1097, 1991
MwoI GCNNNNNNNGC 3 cut(s) 38, 689, 903
NarI GGCGCC 1 cut(s) 1333
NciI CCSGG 1 cut(s) 2165
NdeI CATATG 1 cut(s) 2232
NdeII GATC 7 cut(s) 485, 580, 913, 1568, 2335, 2493, 2652
NlaIII CATG 9 cut(s) 15, 203, 428, 542, 688, 1398, 1479, 1969, 2364
NlaIV GGNNCC 5 cut(s) 409, 598, 1216, 1334, 2621
NmuCI GTSAC 4 cut(s) 205, 545, 1676, 1685
NspI RCATGY 3 cut(s) 1398, 1969, 2364
NspV TTCGAA 2 cut(s) 1300, 1782
OliI CACNNNNGTG 1 cut(s) 1267
PaeI GCATGC 1 cut(s) 1398
PasI CCCWGGG 1 cut(s) 1037
PceI AGGCCT 1 cut(s) 773
PciI ACATGT 2 cut(s) 1965, 2360
PciSI GCTCTTC 2 cut(s) 740, 1085
PctI GAATGC 1 cut(s) 1729
PdmI GAANNNNTTC 2 cut(s) 612, 1296
PfeI GAWTC 4 cut(s) 392, 1259, 1388, 1819
PflMI CCANNNNNTGG 1 cut(s) 1849
PkrI GCNGC 6 cut(s) 40, 121, 139, 158, 194, 2261
PleI GAGTC 3 cut(s) 638, 1932, 2157
PluTI GGCGCC 1 cut(s) 1336
PpsI GAGTC 3 cut(s) 638, 1932, 2157
PpuMI RGGWCCY 1 cut(s) 597
PscI ACATGT 2 cut(s) 1965, 2360
PshAI GACNNNNGTC 1 cut(s) 1968
PshBI ATTAAT 2 cut(s) 1865, 1911
PsiI TTATAA 1 cut(s) 1956
Psp124BI GAGCTC 1 cut(s) 1080
Psp1406I AACGTT 2 cut(s) 1086, 1959
Psp5II RGGWCCY 1 cut(s) 597
Psp6I CCWGG 4 cut(s) 599, 1036, 1095, 1989
PspFI CCCAGC 1 cut(s) 2238
PspGI CCWGG 4 cut(s) 599, 1036, 1095, 1989
PspN4I GGNNCC 5 cut(s) 409, 598, 1216, 1334, 2621
PspPI GGNCC 4 cut(s) 239, 440, 597, 733
PspPPI RGGWCCY 1 cut(s) 597
PstNI CAGNNNCTG 1 cut(s) 38
PsuI RGATCY 1 cut(s) 485
RsaI GTAC 2 cut(s) 1474, 1891
RsaNI GTAC 2 cut(s) 1473, 1890
RseI CAYNNNNRTG 2 cut(s) 543, 1267
SacI GAGCTC 1 cut(s) 1080
SapI GCTCTTC 2 cut(s) 740, 1085
SatI GCNGC 6 cut(s) 39, 120, 138, 157, 193, 2260
Sau3AI GATC 7 cut(s) 485, 580, 913, 1568, 2335, 2493, 2652
Sau96I GGNCC 4 cut(s) 239, 440, 597, 733
SchI GAGTC 3 cut(s) 639, 1932, 2158
ScrFI CCNGG 5 cut(s) 601, 1038, 1097, 1991, 2165
SduI GDGCHC 3 cut(s) 265, 1080, 2622
SfaNI GCATC 1 cut(s) 1734
SfcI CTRYAG 1 cut(s) 1554
SfoI GGCGCC 1 cut(s) 1334
SfuI TTCGAA 2 cut(s) 1300, 1782
SinI GGWCC 3 cut(s) 440, 597, 733
SmiMI CAYNNNNRTG 2 cut(s) 543, 1267
SmlI CTYRAG 3 cut(s) 396, 821, 2155
SmoI CTYRAG 3 cut(s) 396, 821, 2155
SphI GCATGC 1 cut(s) 1398
SseBI AGGCCT 1 cut(s) 773
SsiI CCGC 3 cut(s) 478, 558, 954
SspDI GGCGCC 1 cut(s) 1332
SspI AATATT 1 cut(s) 1208
SspMI CTAG 8 cut(s) 627, 782, 939, 1056, 1313, 1530, 1589, 2204
SstI GAGCTC 1 cut(s) 1080
StuI AGGCCT 1 cut(s) 773
StyD4I CCNGG 5 cut(s) 599, 1036, 1095, 1989, 2163
StyI CCWWGG 1 cut(s) 574
TaaI ACNGT 7 cut(s) 168, 250, 331, 1121, 1621, 1658, 1685
TaiI ACGT 6 cut(s) 1089, 1271, 1429, 1842, 1962, 2124
TaqI TCGA 4 cut(s) 95, 1300, 1782, 1929
TaqII GACCGA 3 cut(s) 612, 935, 1773
TatI WGTACW 2 cut(s) 1472, 1889
TfiI GAWTC 4 cut(s) 392, 1259, 1388, 1819
TscAI CASTG 3 cut(s) 105, 171, 2051
TseFI GTSAC 4 cut(s) 205, 545, 1676, 1685
TseI GCWGC 6 cut(s) 38, 119, 137, 156, 192, 2259
Tsp45I GTSAC 4 cut(s) 205, 545, 1676, 1685
TspGWI ACGGA 5 cut(s) 446, 482, 578, 1860, 1913
TspRI CASTG 3 cut(s) 105, 171, 2051
Van91I CCANNNNNTGG 1 cut(s) 1849
Vha464I CTTAAG 1 cut(s) 2155
VpaK11BI GGWCC 3 cut(s) 440, 597, 733
VspI ATTAAT 2 cut(s) 1865, 1911
XapI RAATTY 4 cut(s) 275, 526, 715, 792
XbaI TCTAGA 1 cut(s) 626
XceI RCATGY 3 cut(s) 1398, 1969, 2364
XmnI GAANNNNTTC 2 cut(s) 612, 1296
XspI CTAG 8 cut(s) 627, 782, 939, 1056, 1313, 1530, 1589, 2204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.