pycom07g10670

Rhamnogalacturonate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000179
Physical Location & Seq
Reverse (-)
10856892 .. 10857698
807 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g10670.1

Sequence Viewer

Length: 666 bp
ATGCATTGGGAGTCTTGTACCCGTGCGTGTACAAATGCTTCTATCACCCTGCCGGCCAAAGACGAGTTCAAGAGTAGTCCAGTAACCCGTGCTTACGCAAGTTGGTGGTCAAAGGTATATTATGAGAATTTGGGGATGGCAAGTGGTACCAATTCTTCTCATTCACACCTTAATGTATTGAAGGAGGGGTGTTCTGTGGTTCCTACACAACTGATATCTTTTGATTGCGCGAGTGCTGCTACTTTGCAAGATAGACCAGTGGTTTTAGTCCCTCAATGCCCACGCTTATCTTCTCAACATCTGGATGTATTTGAAGAGGCTGGTGATGAAGTTGCTTCGCACGAAGATGGACTTGTTTTGCGGCAGGGTCAACAAGTTTTAAACAAGCGACGTCTTGACGGCGCTCAGGCCGATAGCGATGTCAATTTTCGTCATAAGAAGAAAGAAGTGTTTAGGCTCCCTCAACTCGATGGCAGTATTTATTCTTATACGGAGATGCTATTTGAAGGCGACCTACCTACAGGCGAGGAGATTGGGGATCCACCTGGTACAGAGCTTATCCCAAGTTCGTCAAGTTTCCCAAGTTTGCCGTGTGTAGGTGGAGGCATGCAAGATCCCGTCATGCATTCTATACCTTTGCCAGCTAGCTCTGATCCATCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

24.07

Weight (kDa)

4.99

Isoelectric Point (pI)

63.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000179)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18561 FvH4_1g29681 FvH4_2g15190 FvH4_3g22800 FvH4_3g22801 FvH4_3g26700 FvH4_4g15162 FvH4_4g15163 FvH4_4g15164 FvH4_4g22273 FvH4_7g02320 FvH4_7g03670 FvH4_7g03671
malus_domestica MD07G1097100.v1.1 MD11G1208600.v1.1 MD15G1303300.v1.1
prunus_persica Prupe.2G070800_v2.0.a1
pyrus_communis pycom02g13430 pycom04g06950 pycom04g09420 pycom04g09610 pycom06g05740 pycom07g00010 pycom07g06720 pycom07g09960 pycom07g10670 pycom07g10680 pycom07g10920 pycom07g27810 pycom08g12110 pycom08g13640 pycom09g03570 pycom09g13470 pycom09g13510 pycom10g01180 pycom10g02180 pycom10g06840 pycom12424g00130 pycom12g03090 pycom12g09570 pycom12g09580 pycom12g09590 pycom1310g00020 pycom13g25030 pycom13g25310 pycom13g25700 pycom13g26160 pycom13g27790 pycom13g28780 pycom13g28790 pycom1683g00070 pycom16g19330 pycom16g21210 pycom16g21710 pycom16g23400 pycom16g24730 pycom17g17550 pycom17g27670 pycom420g00110 pycom420g00130 pycom420g00610 pycom520g00600 pycom675g00050
rosa_chinensis RchiOBHm_Chr1g0316731
rosa_laevigata RLG00000030467
rosa_multiflora Rmu_co8112106.1_g000001 Rmu_co8301911.1_g000001 Rmu_sc0000161.1_g000042 Rmu_sc0000161.1_g000043 Rmu_sc0000173.1_g000035 Rmu_sc0000173.1_g000036 Rmu_sc0000480.1_g000024 Rmu_sc0000670.1_g000020 Rmu_sc0000670.1_g000021 Rmu_sc0000962.1_g000043 Rmu_sc0000962.1_g000044 Rmu_sc0001025.1_g000004 Rmu_sc0001075.1_g000026 Rmu_sc0001075.1_g000027 Rmu_sc0001167.1_g000050 Rmu_sc0001227.1_g000043 Rmu_sc0001346.1_g000006 Rmu_sc0001438.1_g000034 Rmu_sc0001946.1_g000002 Rmu_sc0001981.1_g000005 Rmu_sc0002106.1_g000005 Rmu_sc0002187.1_g000004 Rmu_sc0002187.1_g000005 Rmu_sc0002187.1_g000018 Rmu_sc0002539.1_g000097 Rmu_sc0003008.1_g000050 Rmu_sc0003008.1_g000051 Rmu_sc0003069.1_g000033 Rmu_sc0003069.1_g000034 Rmu_sc0003412.1_g000016 Rmu_sc0003553.1_g000005 Rmu_sc0003553.1_g000006 Rmu_sc0003641.1_g000025 Rmu_sc0003652.1_g000006 Rmu_sc0003687.1_g000019 Rmu_sc0003687.1_g000020 Rmu_sc0004234.1_g000013 Rmu_sc0004249.1_g000005 Rmu_sc0005044.1_g000027 Rmu_sc0005060.1_g000012 Rmu_sc0005816.1_g000005 Rmu_sc0006175.1_g000006 Rmu_sc0006175.1_g000007 Rmu_sc0006695.1_g000046 Rmu_sc0006725.1_g000014 Rmu_sc0006746.1_g000015 Rmu_sc0006746.1_g000016 Rmu_sc0006912.1_g000008 Rmu_sc0007355.1_g000004 Rmu_sc0007705.1_g000001 Rmu_sc0007903.1_g000003 Rmu_sc0007953.1_g000001 Rmu_sc0008630.1_g000001 Rmu_sc0008923.1_g000018 Rmu_sc0008923.1_g000019 Rmu_sc0009186.1_g000003 Rmu_sc0010184.1_g000007 Rmu_sc0010243.1_g000001 Rmu_sc0011242.1_g000004 Rmu_sc0012849.1_g000001 Rmu_sc0012849.1_g000002 Rmu_sc0012883.1_g000001 Rmu_sc0013028.1_g000015 Rmu_sc0015042.1_g000003 Rmu_sc0016730.1_g000002 Rmu_sc0017702.1_g000002 Rmu_sc0017791.1_g000007 Rmu_sc0017874.1_g000005 Rmu_sc0025334.1_g000001 Rmu_sc0025334.1_g000002 Rmu_sc0028789.1_g000001 Rmu_sc0030538.1_g000001 Rmu_sc0033137.1_g000001 Rmu_ssc0000183.1_g000013 Rmu_ssc0000259.1_g000061 Rmu_ssc0000259.1_g000062 Rmu_ssc0000259.1_g000063
rosa_roxburghii Rroxscaffold_173G00435030 Rroxscaffold_173G00435040 Rroxscaffold_1G00033030 Rroxscaffold_2G00093360 Rroxscaffold_2G00113080 Rroxscaffold_2G00146180 Rroxscaffold_3G00227920 Rroxscaffold_3G00251750 Rroxscaffold_4G00328240 Rroxscaffold_5G00333920 Rroxscaffold_5G00338240 Rroxscaffold_5G00342700 Rroxscaffold_6G00412810 Rroxscaffold_6G00423130 Rroxscaffold_7G00175190
rosa_rugosa Rorug01G0029200 Rorug01G0029300 Rorug01G0029400 Rorug01G0029400
rosa_samantha Rh1AG040900 Rh1BG038100 Rh1CG042000 Rh1DG027900
rosa_wichuraiana Rw0G019970 Rw1G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 394
Acc65I GGTACC 1 cut(s) 146
AccB1I GGYRCC 1 cut(s) 146
AccII CGCG 1 cut(s) 230
AciI CCGC 1 cut(s) 361
AclWI GGATC 4 cut(s) 533, 546, 608, 647
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 127
AcyI GRCGYC 1 cut(s) 391
AfaI GTAC 4 cut(s) 19, 31, 148, 550
AfiI CCNNNNNNNGG 1 cut(s) 596
AgsI TTSAA 4 cut(s) 70, 181, 314, 506
AjnI CCWGG 1 cut(s) 544
AjuI GAANNNNNNNTTGG 2 cut(s) 50, 82
AluBI AGCT 3 cut(s) 556, 644, 648
AluI AGCT 3 cut(s) 556, 644, 648
AlwI GGATC 4 cut(s) 533, 546, 608, 647
AoxI GGCC 2 cut(s) 54, 408
ApeKI GCWGC 1 cut(s) 236
ApoI RAATTY 1 cut(s) 127
Asp718I GGTACC 1 cut(s) 146
AspLEI GCGC 2 cut(s) 230, 404
AsuHPI GGTGA 2 cut(s) 37, 335
AsuNHI GCTAGC 1 cut(s) 644
BamHI GGATCC 1 cut(s) 538
BanI GGYRCC 1 cut(s) 146
BarI GAAGNNNNNNTAC 6 cut(s) 22, 54, 139, 171, 498, 530
BbvI GCAGC 1 cut(s) 223
BccI CCATC 4 cut(s) 130, 341, 464, 664
BceAI ACGGC 2 cut(s) 415, 574
BciT130I CCWGG 1 cut(s) 546
BfaI CTAG 1 cut(s) 645
BfmI CTRYAG 1 cut(s) 519
BfoI RGCGCY 1 cut(s) 405
BisI GCNGC 2 cut(s) 237, 362
BlsI GCNGC 2 cut(s) 238, 363
Bme1390I CCNGG 1 cut(s) 546
BmiI GGNNCC 4 cut(s) 148, 201, 458, 540
BmrFI CCNGG 1 cut(s) 546
BmsI GCATC 1 cut(s) 486
BmtI GCTAGC 1 cut(s) 648
Bpu10I CCTNAGC 1 cut(s) 405
BsaHI GRCGYC 1 cut(s) 391
Bsc4I CCNNNNNNNGG 1 cut(s) 596
Bse118I RCCGGY 1 cut(s) 52
Bse1I ACTGG 2 cut(s) 80, 257
BseBI CCWGG 1 cut(s) 546
BseGI GGATG 2 cut(s) 141, 310
BseLI CCNNNNNNNGG 1 cut(s) 596
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 2 cut(s) 80, 257
BseRI GAGGAG 1 cut(s) 542
BseXI GCAGC 1 cut(s) 223
Bsh1236I CGCG 1 cut(s) 230
BshFI GGCC 2 cut(s) 56, 410
BshNI GGYRCC 1 cut(s) 146
BsiSI CCGG 1 cut(s) 53
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 1 cut(s) 596
BsmFI GGGAC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 2 cut(s) 56, 410
Bsp1407I TGTACA 1 cut(s) 29
Bsp143I GATC 3 cut(s) 538, 613, 652
BspACI CCGC 1 cut(s) 361
BspANI GGCC 2 cut(s) 56, 410
BspCNI CTCAG 1 cut(s) 418
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 4 cut(s) 148, 201, 458, 540
BspOI GCTAGC 1 cut(s) 648
BspPI GGATC 4 cut(s) 533, 546, 608, 647
BspT107I GGYRCC 1 cut(s) 146
BsrFI RCCGGY 1 cut(s) 52
BsrGI TGTACA 1 cut(s) 29
BsrI ACTGG 2 cut(s) 80, 257
BssAI RCCGGY 1 cut(s) 52
BssMI GATC 3 cut(s) 538, 613, 652
BssNI GRCGYC 1 cut(s) 391
Bst2UI CCWGG 1 cut(s) 546
Bst6I CTCTTC 1 cut(s) 309
BstACI GRCGYC 1 cut(s) 391
BstAUI TGTACA 1 cut(s) 29
BstC8I GCNNGC 4 cut(s) 54, 608, 642, 646
BstDEI CTNAG 1 cut(s) 405
BstF5I GGATG 2 cut(s) 141, 310
BstFNI CGCG 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 2 cut(s) 230, 404
BstKTI GATC 3 cut(s) 541, 616, 655
BstMBI GATC 3 cut(s) 538, 613, 652
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNI CCWGG 1 cut(s) 546
BstNSI RCATGY 1 cut(s) 610
BstSCI CCNGG 1 cut(s) 544
BstSFI CTRYAG 1 cut(s) 519
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 1 cut(s) 223
BstX2I RGATCY 2 cut(s) 538, 613
BstYI RGATCY 2 cut(s) 538, 613
BsuRI GGCC 2 cut(s) 56, 410
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 2 cut(s) 141, 310
BtsIMutI CAGTG 1 cut(s) 264
Cac8I GCNNGC 4 cut(s) 54, 608, 642, 646
CfoI GCGC 2 cut(s) 230, 404
Cfr10I RCCGGY 1 cut(s) 52
CsiI ACCWGGT 1 cut(s) 544
Csp6I GTAC 4 cut(s) 18, 30, 147, 549
CviAII CATG 2 cut(s) 607, 622
CviJI RGCY 7 cut(s) 56, 320, 410, 457, 556, 644, 648
CviKI_1 RGCY 7 cut(s) 56, 320, 410, 457, 556, 644, 648
CviQI GTAC 4 cut(s) 18, 30, 147, 549
DdeI CTNAG 1 cut(s) 405
DpnI GATC 3 cut(s) 540, 615, 654
DpnII GATC 3 cut(s) 538, 613, 652
DraI TTTAAA 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
Eco32I GATATC 1 cut(s) 216
EcoRII CCWGG 1 cut(s) 544
EcoRV GATATC 1 cut(s) 216
EcoT22I ATGCAT 2 cut(s) 6, 627
FaeI CATG 2 cut(s) 610, 625
FaiI YATR 7 cut(s) 118, 123, 435, 489, 608, 623, 632
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 1 cut(s) 254
FatI CATG 2 cut(s) 606, 621
Fnu4HI GCNGC 2 cut(s) 237, 362
FokI GGATG 2 cut(s) 148, 317
Fsp4HI GCNGC 2 cut(s) 237, 362
FspBI CTAG 1 cut(s) 645
GlaI GCGC 2 cut(s) 229, 403
GluI GCNGC 2 cut(s) 237, 362
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 2 cut(s) 56, 410
HapII CCGG 1 cut(s) 53
HhaI GCGC 2 cut(s) 230, 404
Hin1I GRCGYC 1 cut(s) 391
Hin1II CATG 2 cut(s) 610, 625
Hin6I GCGC 2 cut(s) 228, 402
HinP1I GCGC 2 cut(s) 228, 402
HincII GTYRAC 1 cut(s) 371
HindII GTYRAC 1 cut(s) 371
HinfI GANTC 1 cut(s) 11
HpaII CCGG 1 cut(s) 53
HphI GGTGA 2 cut(s) 37, 335
Hpy166II GTNNAC 2 cut(s) 30, 371
Hpy188I TCNGA 2 cut(s) 652, 665
Hpy188III TCNNGA 3 cut(s) 70, 302, 395
Hpy8I GTNNAC 2 cut(s) 30, 371
Hpy99I CGWCG 1 cut(s) 393
HpyAV CCTTC 2 cut(s) 175, 500
HpyCH4IV ACGT 1 cut(s) 391
HpyCH4V TGCA 4 cut(s) 4, 247, 610, 625
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 1 cut(s) 405
HpySE526I ACGT 1 cut(s) 391
Hsp92I GRCGYC 1 cut(s) 391
Hsp92II CATG 2 cut(s) 610, 625
HspAI GCGC 2 cut(s) 228, 402
KpnI GGTACC 1 cut(s) 150
KroI GCCGGC 1 cut(s) 52
KroNI GCCGGC 1 cut(s) 54
Kzo9I GATC 3 cut(s) 538, 613, 652
LmnI GCTCC 1 cut(s) 462
Lsp1109I GCAGC 1 cut(s) 223
LweI GCATC 1 cut(s) 486
MabI ACCWGGT 1 cut(s) 544
MaeI CTAG 1 cut(s) 645
MaeII ACGT 1 cut(s) 391
MaeIII GTNAC 1 cut(s) 82
MalI GATC 3 cut(s) 540, 615, 654
MboI GATC 3 cut(s) 538, 613, 652
MboII GAAGA 5 cut(s) 147, 282, 326, 356, 451
MflI RGATCY 2 cut(s) 538, 613
MluCI AATT 3 cut(s) 127, 151, 424
MlyI GAGTC 1 cut(s) 20
MnlI CCTC 6 cut(s) 178, 282, 310, 471, 520, 596
Mph1103I ATGCAT 2 cut(s) 6, 627
MroNI GCCGGC 1 cut(s) 52
MseI TTAA 2 cut(s) 171, 380
MslI CAYNNNNRTG 3 cut(s) 171, 303, 345
MspI CCGG 1 cut(s) 53
MspR9I CCNGG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 1 cut(s) 546
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 1 cut(s) 236
NaeI GCCGGC 1 cut(s) 54
NdeII GATC 3 cut(s) 538, 613, 652
NgoMIV GCCGGC 1 cut(s) 52
NheI GCTAGC 1 cut(s) 644
NlaIII CATG 2 cut(s) 610, 625
NlaIV GGNNCC 4 cut(s) 148, 201, 458, 540
NsiI ATGCAT 2 cut(s) 6, 627
NspI RCATGY 1 cut(s) 610
PaeI GCATGC 1 cut(s) 610
PctI GAATGC 1 cut(s) 625
PdiI GCCGGC 1 cut(s) 54
PkrI GCNGC 2 cut(s) 238, 363
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 544
PspGI CCWGG 1 cut(s) 544
PspN4I GGNNCC 4 cut(s) 148, 201, 458, 540
PsuI RGATCY 2 cut(s) 538, 613
RsaI GTAC 4 cut(s) 19, 31, 148, 550
RsaNI GTAC 4 cut(s) 18, 30, 147, 549
RseI CAYNNNNRTG 3 cut(s) 171, 303, 345
SaqAI TTAA 2 cut(s) 171, 380
SatI GCNGC 2 cut(s) 237, 362
Sau3AI GATC 3 cut(s) 538, 613, 652
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 1 cut(s) 546
SexAI ACCWGGT 1 cut(s) 544
SfaNI GCATC 1 cut(s) 486
SfcI CTRYAG 1 cut(s) 519
SmiMI CAYNNNNRTG 3 cut(s) 171, 303, 345
SphI GCATGC 1 cut(s) 610
Sse9I AATT 3 cut(s) 127, 151, 424
SsiI CCGC 1 cut(s) 361
SspMI CTAG 1 cut(s) 645
StyD4I CCNGG 1 cut(s) 544
TaiI ACGT 1 cut(s) 394
TaqI TCGA 1 cut(s) 468
TasI AATT 3 cut(s) 127, 151, 424
TatI WGTACW 1 cut(s) 29
TauI GCSGC 1 cut(s) 364
Tru1I TTAA 2 cut(s) 171, 380
Tru9I TTAA 2 cut(s) 171, 380
TscAI CASTG 1 cut(s) 264
TseI GCWGC 1 cut(s) 236
TspDTI ATGAA 1 cut(s) 342
TspGWI ACGGA 1 cut(s) 506
TspRI CASTG 1 cut(s) 264
XapI RAATTY 1 cut(s) 127
XceI RCATGY 1 cut(s) 610
XspI CTAG 1 cut(s) 645
ZraI GACGTC 1 cut(s) 392
Zsp2I ATGCAT 2 cut(s) 6, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.