pycom16g24730

NatA auxiliary

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000179
Physical Location & Seq
Reverse (-)
26357525 .. 26360140
2616 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g24730.3

Sequence Viewer

Length: 1566 bp
ATGGCTTGGAATGATGCCTTGTCGACTACCGGAGAAGCTGTTTTTGATGAGTCTTATTGGGAGTGGCTTGAAGATGTCTTGGGCCGATCTAAACATGTGCTTACTAGCATGGGCCTTTACCATGCCGTGTACGCGTCTTTGTTTAGTTATGATCTCCATCATTCTGTCCTTCATGCGTTCTTCGAGCATTGGTGTTCAGCGACTAACACGCTTCATACAGCTCAAGGAGAGATGTCAATTTCACTTTGGGATCTCCACAAGATAGGGGGATTGCCAATCCAAGGTAAGTTTTATGACGAGGTCGTTCCTAGTGCGGAGGAACTTTCTCTCTGCAATAACCGAGGATTGCCTGCGAGTTGTCGCTGTTTATTTTGGGCATATCACAAGCTTTCCCAAGATGCTCGAAGTAAATCAGGCGTGAAGATTTCTTCATGGATCCGCTTCTGGTATTGGGAGGACACAAGGTACAAGAAGTCCTCGAAGAGAAGAAGTTGCAACAAAACCACTAAGCCAAAGGGAGACTCATATCCATCTGGTGCCATTGGTTCAGCTAGGCGCCGTTCCTCTGCCGAGTTGAGAGCGTTTGAGGACCTTGGCATAGCATCAGAGCATTTTGTTTTTCCAATCGGCGACGTCAACTTTGTTCGTCCTGGGGTTTTCAAAGTTGCTAGCAAGATGGCTGCAGGTGAATCTTTCAGTCTTGCTATCCCGGTTTTGGCCAATATTTATAATGGCTTGAGTGTTGTTTCGAACTCAGCAAACACTGAAGATCGTGCCGCGGTTCTTCTCTACCACTATGTGTATGGTTGGTTGGGTATTTATTCTTATACGGAGATGCTATTTGGAGGCGACCTACCTACAGGCGAGGAGATTGGGGATCCACCTGTTACAGAGCTTATCCCAAGTTCGTCAAGTTTCCCAAGTTTGCTGTGTGTAGGTGGAGGCATGCAAGATCCCGTCATGCATTCTACACCTTTGCCAGCTAGCTCTGAGATCTCTCTGAGAGGGTCAAACCTTAGTGGTACTGAGCAGCTTATTCATCGCATCAATCTTCGTGCGGCAATGGATATCAAGAGCCATATTGAGGGGAGGATTTCGAAATGCCCGTCGGAGGACCTTGCGTTGCTTAAGGAGGACTTGTCGAAGCTTATTTCTGCAATTGACAATCTTAACATTGATTCTTCATCCTTGAAGATCAGGATTGCCGAAATTATGGCCGCCTCAACTGAGTATTCTTCCCTGCGTGTTATTTCCTCGAAGAAGTTGAGTCCAGATGTTAGAGCTCACCAACTTGCTGCAATAGACTTGTCACTAACCCAAGCCCGGTCTTCTCAGCAAGCTGCTTCGGGAGATTATCAATCTACAGAGACTTCTTTAGCTTCCGTCCGGATACGTCTAGATACGTTGATGCGAGAACAAGAACAGCTGGGCACCGAGGCATCAAAACTTGAAAGTGTTCTCGGGGAGCAGGGAGCTACACTTTCTCAATGCCAAGAGGAGATTTCACGCCTAGAACAAGAGAAGACATGGCTATGGAGTTGCCCATCCTGTCTCTCACCGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NARP1 TPR_11 TPR_2

Protein Analysis

522

Amino Acids

57.56

Weight (kDa)

5.87

Isoelectric Point (pI)

58.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000179)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18561 FvH4_1g29681 FvH4_2g15190 FvH4_3g22800 FvH4_3g22801 FvH4_3g26700 FvH4_4g15162 FvH4_4g15163 FvH4_4g15164 FvH4_4g22273 FvH4_7g02320 FvH4_7g03670 FvH4_7g03671
malus_domestica MD07G1097100.v1.1 MD11G1208600.v1.1 MD15G1303300.v1.1
prunus_persica Prupe.2G070800_v2.0.a1
pyrus_communis pycom02g13430 pycom04g06950 pycom04g09420 pycom04g09610 pycom06g05740 pycom07g00010 pycom07g06720 pycom07g09960 pycom07g10670 pycom07g10680 pycom07g10920 pycom07g27810 pycom08g12110 pycom08g13640 pycom09g03570 pycom09g13470 pycom09g13510 pycom10g01180 pycom10g02180 pycom10g06840 pycom12424g00130 pycom12g03090 pycom12g09570 pycom12g09580 pycom12g09590 pycom1310g00020 pycom13g25030 pycom13g25310 pycom13g25700 pycom13g26160 pycom13g27790 pycom13g28780 pycom13g28790 pycom1683g00070 pycom16g19330 pycom16g21210 pycom16g21710 pycom16g23400 pycom16g24730 pycom17g17550 pycom17g27670 pycom420g00110 pycom420g00130 pycom420g00610 pycom520g00600 pycom675g00050
rosa_chinensis RchiOBHm_Chr1g0316731
rosa_laevigata RLG00000030467
rosa_multiflora Rmu_co8112106.1_g000001 Rmu_co8301911.1_g000001 Rmu_sc0000161.1_g000042 Rmu_sc0000161.1_g000043 Rmu_sc0000173.1_g000035 Rmu_sc0000173.1_g000036 Rmu_sc0000480.1_g000024 Rmu_sc0000670.1_g000020 Rmu_sc0000670.1_g000021 Rmu_sc0000962.1_g000043 Rmu_sc0000962.1_g000044 Rmu_sc0001025.1_g000004 Rmu_sc0001075.1_g000026 Rmu_sc0001075.1_g000027 Rmu_sc0001167.1_g000050 Rmu_sc0001227.1_g000043 Rmu_sc0001346.1_g000006 Rmu_sc0001438.1_g000034 Rmu_sc0001946.1_g000002 Rmu_sc0001981.1_g000005 Rmu_sc0002106.1_g000005 Rmu_sc0002187.1_g000004 Rmu_sc0002187.1_g000005 Rmu_sc0002187.1_g000018 Rmu_sc0002539.1_g000097 Rmu_sc0003008.1_g000050 Rmu_sc0003008.1_g000051 Rmu_sc0003069.1_g000033 Rmu_sc0003069.1_g000034 Rmu_sc0003412.1_g000016 Rmu_sc0003553.1_g000005 Rmu_sc0003553.1_g000006 Rmu_sc0003641.1_g000025 Rmu_sc0003652.1_g000006 Rmu_sc0003687.1_g000019 Rmu_sc0003687.1_g000020 Rmu_sc0004234.1_g000013 Rmu_sc0004249.1_g000005 Rmu_sc0005044.1_g000027 Rmu_sc0005060.1_g000012 Rmu_sc0005816.1_g000005 Rmu_sc0006175.1_g000006 Rmu_sc0006175.1_g000007 Rmu_sc0006695.1_g000046 Rmu_sc0006725.1_g000014 Rmu_sc0006746.1_g000015 Rmu_sc0006746.1_g000016 Rmu_sc0006912.1_g000008 Rmu_sc0007355.1_g000004 Rmu_sc0007705.1_g000001 Rmu_sc0007903.1_g000003 Rmu_sc0007953.1_g000001 Rmu_sc0008630.1_g000001 Rmu_sc0008923.1_g000018 Rmu_sc0008923.1_g000019 Rmu_sc0009186.1_g000003 Rmu_sc0010184.1_g000007 Rmu_sc0010243.1_g000001 Rmu_sc0011242.1_g000004 Rmu_sc0012849.1_g000001 Rmu_sc0012849.1_g000002 Rmu_sc0012883.1_g000001 Rmu_sc0013028.1_g000015 Rmu_sc0015042.1_g000003 Rmu_sc0016730.1_g000002 Rmu_sc0017702.1_g000002 Rmu_sc0017791.1_g000007 Rmu_sc0017874.1_g000005 Rmu_sc0025334.1_g000001 Rmu_sc0025334.1_g000002 Rmu_sc0028789.1_g000001 Rmu_sc0030538.1_g000001 Rmu_sc0033137.1_g000001 Rmu_ssc0000183.1_g000013 Rmu_ssc0000259.1_g000061 Rmu_ssc0000259.1_g000062 Rmu_ssc0000259.1_g000063
rosa_roxburghii Rroxscaffold_173G00435030 Rroxscaffold_173G00435040 Rroxscaffold_1G00033030 Rroxscaffold_2G00093360 Rroxscaffold_2G00113080 Rroxscaffold_2G00146180 Rroxscaffold_3G00227920 Rroxscaffold_3G00251750 Rroxscaffold_4G00328240 Rroxscaffold_5G00333920 Rroxscaffold_5G00338240 Rroxscaffold_5G00342700 Rroxscaffold_6G00412810 Rroxscaffold_6G00423130 Rroxscaffold_7G00175190
rosa_rugosa Rorug01G0029200 Rorug01G0029300 Rorug01G0029400 Rorug01G0029400
rosa_samantha Rh1AG040900 Rh1BG038100 Rh1CG042000 Rh1DG027900
rosa_wichuraiana Rw0G019970 Rw1G003400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
AarI CACCTGC 1 cut(s) 674
AatII GACGTC 1 cut(s) 636
Acc36I ACCTGC 1 cut(s) 674
AccB1I GGYRCC 3 cut(s) 536, 555, 1430
AccI GTMKAC 1 cut(s) 23
AccII CGCG 2 cut(s) 134, 779
AccIII TCCGGA 1 cut(s) 1386
AciI CCGC 6 cut(s) 314, 439, 777, 779, 1058, 1218
AclWI GGATC 6 cut(s) 258, 430, 443, 872, 885, 947
AcoI YGGCCR 2 cut(s) 717, 1215
AcuI CTGAAG 1 cut(s) 786
AcyI GRCGYC 2 cut(s) 556, 633
AdeI CACNNNGTG 1 cut(s) 799
AfaI GTAC 3 cut(s) 131, 467, 1024
AfiI CCNNNNNNNGG 5 cut(s) 281, 715, 1084, 1111, 1323
AflII CTTAAG 1 cut(s) 1127
AflIII ACRYGT 2 cut(s) 94, 132
AgsI TTSAA 4 cut(s) 71, 661, 1192, 1451
AjnI CCWGG 1 cut(s) 649
Alw21I GWGCWC 1 cut(s) 1285
Alw26I GTCTC 3 cut(s) 513, 1361, 1556
AlwI GGATC 6 cut(s) 258, 430, 443, 872, 885, 947
Ama87I CYCGRG 1 cut(s) 1460
Aor13HI TCCGGA 1 cut(s) 1386
AoxI GGCC 4 cut(s) 82, 112, 717, 1215
ApeKI GCWGC 4 cut(s) 680, 1030, 1295, 1340
ArsI GACNNNNNNTTYG 4 cut(s) 623, 655, 1091, 1123
Asp700I GAANNNNTTC 1 cut(s) 1455
AspLEI GCGC 1 cut(s) 558
AspS9I GGNCC 4 cut(s) 82, 112, 589, 1114
AsuC2I CCSGG 2 cut(s) 710, 1324
AsuHPI GGTGA 3 cut(s) 698, 1277, 1548
AsuII TTCGAA 2 cut(s) 749, 1097
AsuNHI GCTAGC 2 cut(s) 668, 983
AvaI CYCGRG 1 cut(s) 1460
AvaII GGWCC 2 cut(s) 589, 1114
BaeGI GKGCMC 1 cut(s) 1433
BalI TGGCCA 1 cut(s) 719
BamHI GGATCC 2 cut(s) 435, 877
BanI GGYRCC 3 cut(s) 536, 555, 1430
BanII GRGCYC 1 cut(s) 1285
BbsI GAAGAC 2 cut(s) 1320, 1529
Bbv12I GWGCWC 1 cut(s) 1285
BbvI GCAGC 4 cut(s) 667, 1042, 1282, 1327
BccI CCATC 4 cut(s) 165, 538, 670, 1552
BceAI ACGGC 2 cut(s) 110, 543
BciT130I CCWGG 1 cut(s) 651
BciVI GTATCC 1 cut(s) 1383
BcnI CCSGG 2 cut(s) 710, 1324
BcoDI GTCTC 3 cut(s) 513, 1361, 1556
BfaI CTAG 7 cut(s) 105, 309, 552, 669, 984, 1397, 1511
BfmI CTRYAG 3 cut(s) 681, 858, 1362
BfoI RGCGCY 1 cut(s) 559
BfrI CTTAAG 1 cut(s) 1127
BfuAI ACCTGC 1 cut(s) 674
BfuI GTATCC 1 cut(s) 1383
BglII AGATCT 1 cut(s) 993
BisI GCNGC 7 cut(s) 681, 777, 1031, 1059, 1218, 1296, 1341
BlsI GCNGC 7 cut(s) 682, 778, 1032, 1060, 1219, 1297, 1342
Bme1390I CCNGG 3 cut(s) 651, 710, 1324
Bme18I GGWCC 2 cut(s) 589, 1114
BmeT110I CYCGRG 1 cut(s) 1460
BmgT120I GGNCC 4 cut(s) 82, 112, 589, 1114
BmiI GGNNCC 5 cut(s) 437, 538, 557, 879, 1432
BmrFI CCNGG 3 cut(s) 651, 710, 1324
BmsI GCATC 7 cut(s) 4, 388, 611, 825, 1053, 1398, 1448
BmtI GCTAGC 2 cut(s) 672, 987
BpiI GAAGAC 2 cut(s) 1320, 1529
Bpu14I TTCGAA 2 cut(s) 749, 1097
BpuEI CTTGAG 2 cut(s) 207, 757
BpuMI CCSGG 2 cut(s) 710, 1324
BsaBI GATNNNNATC 1 cut(s) 156
BsaHI GRCGYC 2 cut(s) 556, 633
BsaJI CCNNGG 7 cut(s) 280, 340, 592, 650, 777, 1434, 1559
BsaWI WCCGGW 2 cut(s) 29, 1386
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 1124, 1154
Bsc4I CCNNNNNNNGG 5 cut(s) 281, 715, 1084, 1111, 1323
Bse3DI GCAATG 1 cut(s) 1068
Bse8I GATNNNNATC 1 cut(s) 156
BseAI TCCGGA 1 cut(s) 1386
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 7 cut(s) 280, 340, 592, 650, 777, 1434, 1559
BseGI GGATG 2 cut(s) 1184, 1544
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 5 cut(s) 281, 715, 1084, 1111, 1323
BseMI GCAATG 1 cut(s) 1068
BseMII CTCAG 6 cut(s) 768, 981, 992, 1017, 1218, 1346
BseRI GAGGAG 2 cut(s) 881, 1511
BseSI GKGCMC 1 cut(s) 1433
BseXI GCAGC 4 cut(s) 667, 1042, 1282, 1327
BseYI CCCAGC 1 cut(s) 1426
Bsh1236I CGCG 2 cut(s) 134, 779
BshFI GGCC 4 cut(s) 84, 114, 719, 1217
BshNI GGYRCC 3 cut(s) 536, 555, 1430
BsiHKAI GWGCWC 1 cut(s) 1285
BsiHKCI CYCGRG 1 cut(s) 1460
BsiSI CCGG 4 cut(s) 30, 710, 1324, 1387
BslI CCNNNNNNNGG 5 cut(s) 281, 715, 1084, 1111, 1323
BsmAI GTCTC 3 cut(s) 513, 1361, 1556
BsmI GAATGC 1 cut(s) 964
BsnI GGCC 4 cut(s) 84, 114, 719, 1217
BsoBI CYCGRG 1 cut(s) 1460
Bsp119I TTCGAA 2 cut(s) 749, 1097
Bsp1286I GDGCHC 2 cut(s) 1285, 1433
Bsp13I TCCGGA 1 cut(s) 1386
Bsp143I GATC 9 cut(s) 86, 151, 250, 435, 769, 877, 952, 993, 1194
BspACI CCGC 6 cut(s) 314, 439, 777, 779, 1058, 1218
BspANI GGCC 4 cut(s) 84, 114, 719, 1217
BspCNI CTCAG 6 cut(s) 767, 982, 993, 1018, 1219, 1345
BspEI TCCGGA 1 cut(s) 1386
BspFNI CGCG 2 cut(s) 134, 779
BspLI GGNNCC 5 cut(s) 437, 538, 557, 879, 1432
BspMAI CTGCAG 1 cut(s) 685
BspMI ACCTGC 1 cut(s) 674
BspOI GCTAGC 2 cut(s) 672, 987
BspPI GGATC 6 cut(s) 258, 430, 443, 872, 885, 947
BspT104I TTCGAA 2 cut(s) 749, 1097
BspT107I GGYRCC 3 cut(s) 536, 555, 1430
BspTI CTTAAG 1 cut(s) 1127
BsrDI GCAATG 1 cut(s) 1068
BssECI CCNNGG 7 cut(s) 280, 340, 592, 650, 777, 1434, 1559
BssMI GATC 9 cut(s) 86, 151, 250, 435, 769, 877, 952, 993, 1194
BssNI GRCGYC 2 cut(s) 556, 633
BssT1I CCWWGG 2 cut(s) 280, 592
Bst2UI CCWGG 1 cut(s) 651
Bst6I CTCTTC 1 cut(s) 476
BstACI GRCGYC 2 cut(s) 556, 633
BstAFI CTTAAG 1 cut(s) 1127
BstBI TTCGAA 2 cut(s) 749, 1097
BstC8I GCNNGC 6 cut(s) 351, 670, 947, 981, 985, 1338
BstDEI CTNAG 8 cut(s) 507, 754, 990, 1001, 1016, 1026, 1227, 1332
BstDSI CCRYGG 1 cut(s) 777
BstF5I GGATG 2 cut(s) 1184, 1544
BstFNI CGCG 2 cut(s) 134, 779
BstH2I RGCGCY 1 cut(s) 559
BstHHI GCGC 1 cut(s) 558
BstKTI GATC 9 cut(s) 89, 154, 253, 438, 772, 880, 955, 996, 1197
BstMAI GTCTC 3 cut(s) 513, 1361, 1556
BstMBI GATC 9 cut(s) 86, 151, 250, 435, 769, 877, 952, 993, 1194
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 1 cut(s) 651
BstNSI RCATGY 2 cut(s) 98, 949
BstSCI CCNGG 3 cut(s) 649, 708, 1322
BstSFI CTRYAG 3 cut(s) 681, 858, 1362
BstSLI GKGCMC 1 cut(s) 1433
BstUI CGCG 2 cut(s) 134, 779
BstV1I GCAGC 4 cut(s) 667, 1042, 1282, 1327
BstV2I GAAGAC 2 cut(s) 1320, 1529
BstX2I RGATCY 5 cut(s) 250, 435, 877, 952, 993
BstYI RGATCY 5 cut(s) 250, 435, 877, 952, 993
BsuI GTATCC 1 cut(s) 1383
BsuRI GGCC 4 cut(s) 84, 114, 719, 1217
BtgI CCRYGG 1 cut(s) 777
BtgZI GCGATG 1 cut(s) 1025
BtsCI GGATG 2 cut(s) 1184, 1544
BtsIMutI CAGTG 1 cut(s) 762
BveI ACCTGC 1 cut(s) 674
Cac8I GCNNGC 6 cut(s) 351, 670, 947, 981, 985, 1338
CfoI GCGC 1 cut(s) 558
Cfr13I GGNCC 4 cut(s) 82, 112, 589, 1114
Cfr42I CCGCGG 1 cut(s) 780
CseI GACGC 1 cut(s) 123
Csp6I GTAC 3 cut(s) 130, 466, 1023
CviAII CATG 8 cut(s) 95, 109, 122, 173, 432, 946, 961, 1527
CviQI GTAC 3 cut(s) 130, 466, 1023
DdeI CTNAG 8 cut(s) 507, 754, 990, 1001, 1016, 1026, 1227, 1332
DinI GGCGCC 1 cut(s) 557
DpnI GATC 9 cut(s) 88, 153, 252, 437, 771, 879, 954, 995, 1196
DpnII GATC 9 cut(s) 86, 151, 250, 435, 769, 877, 952, 993, 1194
DraIII CACNNNGTG 1 cut(s) 799
EaeI YGGCCR 2 cut(s) 717, 1215
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
Ecl136II GAGCTC 1 cut(s) 1283
Eco130I CCWWGG 2 cut(s) 280, 592
Eco24I GRGCYC 1 cut(s) 1285
Eco32I GATATC 1 cut(s) 1069
Eco47I GGWCC 2 cut(s) 589, 1114
Eco53kI GAGCTC 1 cut(s) 1283
Eco57I CTGAAG 1 cut(s) 786
Eco88I CYCGRG 1 cut(s) 1460
EcoICRI GAGCTC 1 cut(s) 1283
EcoO109I RGGNCCY 2 cut(s) 589, 1114
EcoRII CCWGG 1 cut(s) 649
EcoRV GATATC 1 cut(s) 1069
EcoT14I CCWWGG 2 cut(s) 280, 592
EcoT22I ATGCAT 1 cut(s) 966
EcoT38I GRGCYC 1 cut(s) 1285
EgeI GGCGCC 1 cut(s) 557
EheI GGCGCC 1 cut(s) 557
ErhI CCWWGG 2 cut(s) 280, 592
FaeI CATG 8 cut(s) 98, 112, 125, 176, 435, 949, 964, 1530
FalI AAGNNNNNCTT 2 cut(s) 1121, 1153
FatI CATG 8 cut(s) 94, 108, 121, 172, 431, 945, 960, 1526
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 7 cut(s) 681, 777, 1031, 1059, 1218, 1296, 1341
FokI GGATG 2 cut(s) 1171, 1531
FriOI GRGCYC 1 cut(s) 1285
Fsp4HI GCNGC 7 cut(s) 681, 777, 1031, 1059, 1218, 1296, 1341
FspBI CTAG 7 cut(s) 105, 309, 552, 669, 984, 1397, 1511
GlaI GCGC 1 cut(s) 557
GluI GCNGC 7 cut(s) 681, 777, 1031, 1059, 1218, 1296, 1341
GsaI CCCAGC 1 cut(s) 1430
HaeII RGCGCY 1 cut(s) 559
HaeIII GGCC 4 cut(s) 84, 114, 719, 1217
HapII CCGG 4 cut(s) 30, 710, 1324, 1387
HgaI GACGC 1 cut(s) 123
HhaI GCGC 1 cut(s) 558
Hin1I GRCGYC 2 cut(s) 556, 633
Hin1II CATG 8 cut(s) 98, 112, 125, 176, 435, 949, 964, 1530
Hin6I GCGC 1 cut(s) 556
HinP1I GCGC 1 cut(s) 556
HincII GTYRAC 2 cut(s) 24, 637
HindII GTYRAC 2 cut(s) 24, 637
HindIII AAGCTT 2 cut(s) 386, 1145
HinfI GANTC 5 cut(s) 50, 521, 689, 1178, 1267
HpaII CCGG 4 cut(s) 30, 710, 1324, 1387
HphI GGTGA 3 cut(s) 698, 1277, 1548
Hpy166II GTNNAC 3 cut(s) 24, 130, 637
Hpy188I TCNGA 4 cut(s) 607, 991, 1002, 1111
Hpy188III TCNNGA 6 cut(s) 1072, 1198, 1271, 1347, 1387, 1397
Hpy8I GTNNAC 3 cut(s) 24, 130, 637
Hpy99I CGWCG 2 cut(s) 635, 1111
HpyAV CCTTC 1 cut(s) 179
HpyCH4IV ACGT 3 cut(s) 633, 1393, 1403
HpyCH4V TGCA 7 cut(s) 333, 495, 683, 949, 964, 1157, 1298
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 8 cut(s) 507, 754, 990, 1001, 1016, 1026, 1227, 1332
HpySE526I ACGT 3 cut(s) 633, 1393, 1403
Hsp92I GRCGYC 2 cut(s) 556, 633
Hsp92II CATG 8 cut(s) 98, 112, 125, 176, 435, 949, 964, 1530
HspAI GCGC 1 cut(s) 556
KasI GGCGCC 1 cut(s) 555
Kpn2I TCCGGA 1 cut(s) 1386
KspI CCGCGG 1 cut(s) 780
Kzo9I GATC 9 cut(s) 86, 151, 250, 435, 769, 877, 952, 993, 1194
LmnI GCTCC 2 cut(s) 1465, 1472
Lsp1109I GCAGC 4 cut(s) 667, 1042, 1282, 1327
LweI GCATC 7 cut(s) 4, 388, 611, 825, 1053, 1398, 1448
MaeI CTAG 7 cut(s) 105, 309, 552, 669, 984, 1397, 1511
MaeII ACGT 3 cut(s) 633, 1393, 1403
MaeIII GTNAC 2 cut(s) 886, 1308
MalI GATC 9 cut(s) 88, 153, 252, 437, 771, 879, 954, 995, 1196
MboI GATC 9 cut(s) 86, 151, 250, 435, 769, 877, 952, 993, 1194
MfeI CAATTG 1 cut(s) 1158
MflI RGATCY 5 cut(s) 250, 435, 877, 952, 993
MhlI GDGCHC 2 cut(s) 1285, 1433
MlsI TGGCCA 1 cut(s) 719
MluCI AATT 3 cut(s) 237, 1158, 1209
MluI ACGCGT 1 cut(s) 132
MluNI TGGCCA 1 cut(s) 719
Mly113I GGCGCC 1 cut(s) 556
MlyI GAGTC 3 cut(s) 59, 515, 1276
MmeI TCCRAC 1 cut(s) 1089
Mox20I TGGCCA 1 cut(s) 719
Mph1103I ATGCAT 1 cut(s) 966
MroI TCCGGA 1 cut(s) 1386
MroXI GAANNNNTTC 1 cut(s) 1455
MscI TGGCCA 1 cut(s) 719
MseI TTAA 2 cut(s) 1128, 1170
MslI CAYNNNNRTG 1 cut(s) 1531
Msp20I TGGCCA 1 cut(s) 719
MspA1I CMGCKG 2 cut(s) 779, 1426
MspCI CTTAAG 1 cut(s) 1127
MspI CCGG 4 cut(s) 30, 710, 1324, 1387
MspR9I CCNGG 3 cut(s) 651, 710, 1324
MunI CAATTG 1 cut(s) 1158
Mva1269I GAATGC 1 cut(s) 964
MvaI CCWGG 1 cut(s) 651
MvnI CGCG 2 cut(s) 134, 779
MwoI GCNNNNNNNGC 1 cut(s) 131
NarI GGCGCC 1 cut(s) 556
NciI CCSGG 2 cut(s) 710, 1324
NdeII GATC 9 cut(s) 86, 151, 250, 435, 769, 877, 952, 993, 1194
NheI GCTAGC 2 cut(s) 668, 983
NlaIII CATG 8 cut(s) 98, 112, 125, 176, 435, 949, 964, 1530
NlaIV GGNNCC 5 cut(s) 437, 538, 557, 879, 1432
NmeAIII GCCGAG 1 cut(s) 595
NmuCI GTSAC 1 cut(s) 1308
NsiI ATGCAT 1 cut(s) 966
NspI RCATGY 2 cut(s) 98, 949
NspV TTCGAA 2 cut(s) 749, 1097
PaeI GCATGC 1 cut(s) 949
PaqCI CACCTGC 1 cut(s) 674
PciI ACATGT 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 1103
PctI GAATGC 1 cut(s) 964
PdmI GAANNNNTTC 1 cut(s) 1455
PfeI GAWTC 2 cut(s) 689, 1178
PflFI GACNNNGTC 1 cut(s) 299
PkrI GCNGC 7 cut(s) 682, 778, 1032, 1060, 1219, 1297, 1342
PleI GAGTC 3 cut(s) 58, 515, 1275
PluTI GGCGCC 1 cut(s) 559
PpsI GAGTC 3 cut(s) 58, 515, 1275
PpuMI RGGWCCY 2 cut(s) 589, 1114
PscI ACATGT 1 cut(s) 94
PsiI TTATAA 1 cut(s) 729
Psp124BI GAGCTC 1 cut(s) 1285
Psp5II RGGWCCY 2 cut(s) 589, 1114
Psp6I CCWGG 1 cut(s) 649
PspFI CCCAGC 1 cut(s) 1426
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 5 cut(s) 437, 538, 557, 879, 1432
PspPI GGNCC 4 cut(s) 82, 112, 589, 1114
PspPPI RGGWCCY 2 cut(s) 589, 1114
PstI CTGCAG 1 cut(s) 685
PsuI RGATCY 5 cut(s) 250, 435, 877, 952, 993
PsyI GACNNNGTC 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 1426
RsaI GTAC 3 cut(s) 131, 467, 1024
RsaNI GTAC 3 cut(s) 130, 466, 1023
RseI CAYNNNNRTG 1 cut(s) 1531
SacI GAGCTC 1 cut(s) 1285
SacII CCGCGG 1 cut(s) 780
SalI GTCGAC 1 cut(s) 22
SaqAI TTAA 2 cut(s) 1128, 1170
SatI GCNGC 7 cut(s) 681, 777, 1031, 1059, 1218, 1296, 1341
Sau3AI GATC 9 cut(s) 86, 151, 250, 435, 769, 877, 952, 993, 1194
Sau96I GGNCC 4 cut(s) 82, 112, 589, 1114
SchI GAGTC 3 cut(s) 59, 515, 1276
ScrFI CCNGG 3 cut(s) 651, 710, 1324
SduI GDGCHC 2 cut(s) 1285, 1433
SfaNI GCATC 7 cut(s) 4, 388, 611, 825, 1053, 1398, 1448
SfcI CTRYAG 3 cut(s) 681, 858, 1362
SfoI GGCGCC 1 cut(s) 557
Sfr303I CCGCGG 1 cut(s) 780
SfuI TTCGAA 2 cut(s) 749, 1097
SgrBI CCGCGG 1 cut(s) 780
SinI GGWCC 2 cut(s) 589, 1114
SmiMI CAYNNNNRTG 1 cut(s) 1531
SmlI CTYRAG 3 cut(s) 222, 736, 1127
SmoI CTYRAG 3 cut(s) 222, 736, 1127
SphI GCATGC 1 cut(s) 949
Sse9I AATT 3 cut(s) 237, 1158, 1209
SsiI CCGC 6 cut(s) 314, 439, 777, 779, 1058, 1218
SspDI GGCGCC 1 cut(s) 555
SspI AATATT 1 cut(s) 724
SspMI CTAG 7 cut(s) 105, 309, 552, 669, 984, 1397, 1511
SstI GAGCTC 1 cut(s) 1285
StyD4I CCNGG 3 cut(s) 649, 708, 1322
StyI CCWWGG 2 cut(s) 280, 592
TaiI ACGT 3 cut(s) 636, 1396, 1406
TaqI TCGA 8 cut(s) 23, 183, 403, 479, 749, 1097, 1142, 1256
TasI AATT 3 cut(s) 237, 1158, 1209
TauI GCSGC 3 cut(s) 779, 1061, 1220
TfiI GAWTC 2 cut(s) 689, 1178
Tru1I TTAA 2 cut(s) 1128, 1170
Tru9I TTAA 2 cut(s) 1128, 1170
TscAI CASTG 1 cut(s) 769
TseFI GTSAC 1 cut(s) 1308
TseI GCWGC 4 cut(s) 680, 1030, 1295, 1340
Tsp45I GTSAC 1 cut(s) 1308
TspDTI ATGAA 5 cut(s) 161, 203, 420, 1028, 1173
TspGWI ACGGA 2 cut(s) 845, 1372
TspRI CASTG 1 cut(s) 769
Tth111I GACNNNGTC 1 cut(s) 299
Vha464I CTTAAG 1 cut(s) 1127
VpaK11BI GGWCC 2 cut(s) 589, 1114
XbaI TCTAGA 1 cut(s) 1396
XceI RCATGY 2 cut(s) 98, 949
XcmI CCANNNNNNNNNTGG 1 cut(s) 800
XmiI GTMKAC 1 cut(s) 23
XmnI GAANNNNTTC 1 cut(s) 1455
XspI CTAG 7 cut(s) 105, 309, 552, 669, 984, 1397, 1511
ZraI GACGTC 1 cut(s) 634
Zsp2I ATGCAT 1 cut(s) 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.