MD04G1161700.v1.1

Receptor-like protein 12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000047
Physical Location & Seq
Forward (+)
25274513 .. 25277908
3396 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1161700.v1.1.491

Sequence Viewer

Length: 3396 bp
ATGAAAATTTCTCTGTTTTCATGGCTTTTCTTAATACCCTTTTACTACTGTTCCATCAGCATCAACGTATTTCTCGTCTACGGCCAATGTCCCGGCGATCAGCAATCTTTGCTGCTACAATTGAAGAACAATCTCACATTTGACACTGCAACATCTAAGAAACTTGTCAGGTGGAACAACGGCTCGGATCACTGCTCTTGGGAAGGAGTGTCCTGCAAAAAGGGTTGTGTTTCGAGTCTGGACTTGAGCAGCGAGGAAATTACTGGTGGACTTGATAATTCGAGTTCTCTTTTCGGTCTCAAGTCAATCGAGTACCTGAATTTGGCTTACAATAACTTCCAGAATGCTCAGATTCCATCGGAGTTCAAGCAGCTAACCGGTTTGAGTAATCTGAACTTGTCGAATGCTGGCTTTGCGGGTCAGGTTCCGATTGAGATTTCACACCTGACGAGGTTGGTAACTCTTGATTTGTCTACCTTTTATTTATTTTCCCCCGGAACTCCGTCGTTGAATCTTGAGAATCCGAAATTGGATGTGCTGCTTAGGAACTTTTCTGAGCTTGTTGAACTTTATCTTGATGGTGTGAGTATATCAGCACAGGGGACTGAGTGGTGCCAAGCGATATCATCTTCATTGCCAAACTTGAGGGTATTGAGCTTGTCCACTTGTAACCTTTCAGGCCCTATTCATAATTCATTACTGAAGCTTAAGTCGCTCTCAGTTATTCGGATTGAGAACAACAATTTGTCTACTCATGTTCCGGAGTTCTTCTCGAATTTCTCGAATTTGACTTCCTTACGAATCATGAATTCGGGATTAAATGGTTCATTCCCAAAGAAGATCTTCCAGGTGCCGACACTGCAGACTATTGACTTATCCGGTAATCCGCAGCTTCAAGGTTCCTTACCAGAATTTCGGAAGAACGGATCTCTTCGATCGCTGGTTCTCAATGGGGCAAATTTCTCAGGCCAGATGCTTCCGAACTCTATTGGGAACCTCAAATTGCTGTCCAAAATAGATATTGGAAATTGCAATTTCACTGGATCAATCCCAAAGTCAATGGAAGACCTTACACAGTTGGTTTATTTGGACTTGTCGATGAACAAGTTCAATGGTTCAGTTCCATCTTTCAGTATGGCCCAGAATCTAACCCTAATAAATCTTTCTTACAATCAGTTAACGGGTCAGATTAATTCCTCACGCTGGGAAAACCTGACTAATCTGGTGAATCTGGACTTGCGACGCAATCTACTTAATGGGACTATTCCACCATCTGTGTTTTCTCGTCCGATGCTGCAGAAGCTACAGCTTTCTAACAATGAATTTTCTGGTCAGTTGCTTGAATTTGATGCTATATCTGTACTGGACACCCTTGATTTGAGTAGCAACAAGTTGGAAGGGCCAATTCCCATGTCTATCTTGAAATTCCGAGGGCTTAAGATTCTTTTACTAGCTTCAAACAACTTCACTGGCTCTTTTCTTCTGAATGATATTCAGCAGCTAAAAAATCTTTCGAGTCTCGATCTTTCATACAATAGCTTGTCTATTAATTACACTGAGACCAATTCCTCTCATTCTTCATTTCCAAATATTACCACATTGAAGTTAGTTGCTGGCAATTTGAGAAGAATCCCAAGTTTCTTGAGAAATCAATCAAAATTAAGCACTTTGGACCTATCACAAAACCAGATTCATGGTGAGATACCCAACTGGATTTGGAGGCTCAGTAATCTTCTTCAACTAAATCTTTCTTGCAACTCTCTGGTAACCCTACAAGGTCCTTCACTCAATCTCACTTCTAGTTTGTCTATGCTTGACCTTCATTCCAACCAGCTTCAGGGACAAATCCCAATTTTTCCACCATCTGCCGCTTATGTGGATTACTCAAGCAATAATTTCAGCTCTAGCATTCCGCCTGAAATTGGTGATTTCATTTTTTACACTGTGTTCTTCTCTCTTTCAAGCAATCACTTCCACGGGATTATTCCAGAATCAATCTGCAAGGCGCCATATCTTCAGGTTCTTGATTTGTCCAATAATTCTCTAGGTGGAACGATTCCCCGATGCTTGACTGAGATAAGCAGGACACTGGCAGTACTTAATCTAAGGAGAAACAAACTTGATGGCTCTGTCCCTGATAGATTTCCTCAGAGTTGCAGTTTAAAAACTCTCGACCTCAATGGAAATCAGATATCAGGCCTATTTCCAAAATCTCTTGCCAACTGCACAATGTTAGAGGTTCTAAACATGGGAAACAATCAGATAATGGATACCTTTCCTCGCTTGTTGAAGAATATATCCTCCTTGCGTGTCCTTGTTTTGCGATCCAACCATTTTTATGGACAAATTGGATGTAACACGACCAGTGGCCCCTGGCCAAAGCTTCAAATTGTTGACATAGCTCGCAACAATTTTAGTGGTGAAATACCAGGAACATGTTTGAGAACCTGGTCAGCAATGATGGCTGACGAAGATGATGCCATGGCAAAGATTAATCACCTCCGATTTCAAGTTCTGCAGTTCAGTCAGGTATATTATCAGGATGCTATAACAGTTACCACCAAAGGTCTAGAGATGGAATTGGTAAAGATCTTAACAGTCTTCACCTCGATTGACATCTCCTGCAACAATTTCATTGGATCAATACCTGAGGAAGTGGGAGACCTCAAATCACTCTATGGCCTAAACTTGTCCAGTAATGCTTTCACAGGTACAATCCCATCATCACTGGGTAACCTACGACAGCTTGAGTCCTTGGACCTTTCAGACAACAAACTGAGCGGAACAATTCCACAAGAGCTTGTCAAACTTAATTTCCTCTCTGTCCTGGATCTCTCAAACAATCAACTGGAGGGGAGAATTCCAACTGGCACTCAGATTCAATCCTTTTCACCAGATTCTTTCACTGGCAATAAAGGATTGTGTGGGGCACCTTTGAGTCTAACATGCAGTGATAAGAACGCCTCACGAACAGATCAAAACAAAGTGTCAAAAGTTGATTGGCAGTCCATACATACTGGAGTGGGTTATGGAGTGGGAGCAGGAGTTGTTGTTATCCTCTTAATCGTGTGGGAAGAAGGAAGAAACTGGTTAGAGGACAGCATTGACAAAATTCTTCTGGCGATTCTTCCAATGATGGGATATTCTTACAAAACCCGTGCCGAATGGGACGATGAGGAGGAGGAAGACTTGGAAGAAGAGAGCAAGTATATCATGCAAGACTATAGCAGAGACGAGATTGTTTCAGAAGACAGAGTGTTTCGGGGTCCATATTGTGTGTTTTGTTCAAAACTTGATATGAGTAGGAAGAGGGCCATCCATGACCCAAACTGTACATGCCATTTCTCTCCACCTATTACATCTTCTTCTTCCTCTTCGCACTCATTTTCGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

1132

Amino Acids

125.21

Weight (kDa)

5.72

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 72 1.2e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 301 - 411 8.4e-07 Leucine-rich repeat region
LRR_8 PF13855 720 - 779 3.4e-08 Leucine rich repeat
LRR_4 PF12799 720 - 757 3.2e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 869 - 926 5.2e-08 Leucine rich repeat
LRR_14 PF23598 877 - 946 8.9e-06 Leucine-rich repeat region
LRR_8 PF13855 891 - 950 3.6e-08 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000047)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g13590 FvH4_6g10800 FvH4_6g11080 FvH4_6g11081 FvH4_6g11660 FvH4_6g11660 FvH4_6g11660 FvH4_6g11660 FvH4_6g11660 FvH4_6g21370 FvH4_6g21371 FvH4_6g21371 FvH4_6g21371 FvH4_6g21371 FvH4_6g21371 FvH4_6g21371 FvH4_6g21371 FvH4_6g21373 FvH4_6g21374 FvH4_6g21375 FvH4_6g21378 FvH4_7g13570
malus_domestica MD00G1166300.v1.1 MD04G1159400.v1.1 MD04G1159500.v1.1 MD04G1159700.v1.1 MD04G1159800.v1.1 MD04G1160600.v1.1 MD04G1160700.v1.1 MD04G1160800.v1.1 MD04G1161000.v1.1 MD04G1161100.v1.1 MD04G1161200.v1.1 MD04G1161300.v1.1 MD04G1161600.v1.1 MD04G1161700.v1.1 MD04G1210900.v1.1 MD05G1095100.v1.1 MD05G1095300.v1.1 MD05G1095400.v1.1 MD05G1095600.v1.1 MD08G1062000.v1.1 MD08G1062100.v1.1 MD12G1152900.v1.1 MD12G1153300.v1.1 MD12G1172900.v1.1 MD12G1173400.v1.1 MD12G1173600.v1.1 MD12G1173700.v1.1 MD12G1173800.v1.1 MD12G1174000.v1.1 MD12G1174100.v1.1 MD12G1174400.v1.1 MD12G1175000.v1.1
prunus_persica Prupe.6G281000_v2.0.a1 Prupe.6G281100_v2.0.a1 Prupe.6G281200_v2.0.a1 Prupe.6G281200_v2.0.a1 Prupe.6G281200_v2.0.a1 Prupe.6G281200_v2.0.a1 Prupe.6G281300_v2.0.a1 Prupe.6G281400_v2.0.a1 Prupe.6G281400_v2.0.a1 Prupe.6G281400_v2.0.a1 Prupe.6G281500_v2.0.a1 Prupe.6G281500_v2.0.a1 Prupe.6G288800_v2.0.a1 Prupe.7G088700_v2.0.a1 Prupe.7G088700_v2.0.a1 Prupe.8G054300_v2.0.a1 Prupe.8G054400_v2.0.a1
pyrus_communis pycom04g14110 pycom04g14120 pycom04g14130 pycom04g14180 pycom04g14390 pycom04g14410 pycom04g14420 pycom04g14430 pycom04g14450 pycom04g14460 pycom12g14600 pycom12g14610 pycom12g16390 pycom12g16430 pycom12g16460 pycom12g16470 pycom12g16480 pycom12g16490 pycom12g16580 pycom12g16600
rosa_chinensis RchiOBHm_Chr0c32g0501501 RchiOBHm_Chr0c32g0501511 RchiOBHm_Chr0c32g0501521 RchiOBHm_Chr0c39g0503191 RchiOBHm_Chr0c39g0503201 RchiOBHm_Chr0c39g0503231 RchiOBHm_Chr0c39g0503261 RchiOBHm_Chr0c39g0503271 RchiOBHm_Chr0c39g0503311 RchiOBHm_Chr0c39g0503331 RchiOBHm_Chr1g0316151 RchiOBHm_Chr1g0316251 RchiOBHm_Chr1g0316291 RchiOBHm_Chr1g0351861 RchiOBHm_Chr1g0351911 RchiOBHm_Chr1g0353121 RchiOBHm_Chr1g0353151 RchiOBHm_Chr1g0353161 RchiOBHm_Chr1g0353221 RchiOBHm_Chr1g0353251 RchiOBHm_Chr1g0353271 RchiOBHm_Chr1g0353291 RchiOBHm_Chr1g0353341 RchiOBHm_Chr3g0451491 RchiOBHm_Chr3g0461851 RchiOBHm_Chr3g0461931 RchiOBHm_Chr3g0462001 RchiOBHm_Chr3g0462441 RchiOBHm_Chr3g0462461 RchiOBHm_Chr3g0462511 RchiOBHm_Chr3g0463331 RchiOBHm_Chr3g0463341 RchiOBHm_Chr3g0463351 RchiOBHm_Chr3g0477991 RchiOBHm_Chr3g0478021 RchiOBHm_Chr3g0478031 RchiOBHm_Chr3g0478051 RchiOBHm_Chr3g0478091 RchiOBHm_Chr3g0478101 RchiOBHm_Chr3g0478141 RchiOBHm_Chr3g0478161 RchiOBHm_Chr3g0478181 RchiOBHm_Chr3g0478191 RchiOBHm_Chr3g0478311 RchiOBHm_Chr3g0478331 RchiOBHm_Chr3g0478381 RchiOBHm_Chr3g0478431 RchiOBHm_Chr3g0478451 RchiOBHm_Chr3g0478491 RchiOBHm_Chr3g0478511 RchiOBHm_Chr3g0478551 RchiOBHm_Chr3g0478561 RchiOBHm_Chr3g0478581 RchiOBHm_Chr3g0478601 RchiOBHm_Chr3g0481181 RchiOBHm_Chr3g0483751 RchiOBHm_Chr3g0483761 RchiOBHm_Chr3g0483771 RchiOBHm_Chr3g0483781 RchiOBHm_Chr5g0037311 RchiOBHm_Chr5g0039781 RchiOBHm_Chr5g0039851 RchiOBHm_Chr5g0048141 RchiOBHm_Chr6g0257231
rosa_laevigata RLG00000008156 RLG00000019550 RLG00000019551 RLG00000022912 RLG00000022914 RLG00000023271 RLG00000023639 RLG00000023640 RLG00000023644 RLG00000023646 RLG00000023648 RLG00000023650 RLG00000023654 RLG00000023658 RLG00000023660 RLG00000023661 RLG00000023662 RLG00000023664 RLG00000023666 RLG00000023670 RLG00000023671 RLG00000023672 RLG00000023673 RLG00000023882 RLG00000024789 RLG00000024854 RLG00000024855 RLG00000024856 RLG00000024859 RLG00000024860 RLG00000024886 RLG00000024891 RLG00000024892 RLG00000024972 RLG00000028274 RLG00000028275 RLG00000028277 RLG00000028279 RLG00000028281 RLG00000028283 RLG00000028285 RLG00000028287 RLG00000028376 RLG00000030078 RLG00000030682 RLG00000030690 RLG00000033943 RLG00000033946
rosa_multiflora Rmu_co8376673.1_g000001 Rmu_sc0000103.1_g000026 Rmu_sc0000295.1_g000035 Rmu_sc0000923.1_g000003 Rmu_sc0000923.1_g000005 Rmu_sc0001207.1_g000038 Rmu_sc0001207.1_g000054 Rmu_sc0001207.1_g000059 Rmu_sc0001207.1_g000069 Rmu_sc0001207.1_g000073 Rmu_sc0001207.1_g000080 Rmu_sc0001218.1_g000002 Rmu_sc0001218.1_g000019 Rmu_sc0001218.1_g000039 Rmu_sc0001218.1_g000041 Rmu_sc0001218.1_g000042 Rmu_sc0001218.1_g000047 Rmu_sc0001228.1_g000001 Rmu_sc0001333.1_g000006 Rmu_sc0001431.1_g000002 Rmu_sc0001431.1_g000003 Rmu_sc0001487.1_g000001 Rmu_sc0001518.1_g000007 Rmu_sc0001518.1_g000008 Rmu_sc0001933.1_g000003 Rmu_sc0002151.1_g000010 Rmu_sc0002151.1_g000011 Rmu_sc0002531.1_g000014 Rmu_sc0002531.1_g000017 Rmu_sc0003078.1_g000003 Rmu_sc0003078.1_g000006 Rmu_sc0003078.1_g000013 Rmu_sc0003078.1_g000018 Rmu_sc0003078.1_g000020 Rmu_sc0003326.1_g000001 Rmu_sc0003624.1_g000002 Rmu_sc0003624.1_g000010 Rmu_sc0003624.1_g000011 Rmu_sc0003624.1_g000013 Rmu_sc0003624.1_g000014 Rmu_sc0003850.1_g000006 Rmu_sc0003850.1_g000007 Rmu_sc0004308.1_g000017 Rmu_sc0004308.1_g000020 Rmu_sc0004308.1_g000025 Rmu_sc0004778.1_g000001 Rmu_sc0004888.1_g000006 Rmu_sc0005196.1_g000010 Rmu_sc0005196.1_g000011 Rmu_sc0005313.1_g000001 Rmu_sc0005657.1_g000033 Rmu_sc0005657.1_g000044 Rmu_sc0005657.1_g000052 Rmu_sc0005657.1_g000054 Rmu_sc0005657.1_g000059 Rmu_sc0005657.1_g000064 Rmu_sc0005657.1_g000071 Rmu_sc0005767.1_g000001 Rmu_sc0005767.1_g000005 Rmu_sc0005767.1_g000007 Rmu_sc0005767.1_g000023 Rmu_sc0006119.1_g000004 Rmu_sc0006539.1_g000002 Rmu_sc0006651.1_g000017 Rmu_sc0006651.1_g000021 Rmu_sc0006651.1_g000022 Rmu_sc0007105.1_g000001 Rmu_sc0007641.1_g000001 Rmu_sc0007641.1_g000006 Rmu_sc0007728.1_g000003 Rmu_sc0007777.1_g000005 Rmu_sc0007777.1_g000008 Rmu_sc0007777.1_g000010 Rmu_sc0007777.1_g000019 Rmu_sc0007777.1_g000020 Rmu_sc0007777.1_g000022 Rmu_sc0008105.1_g000002 Rmu_sc0008340.1_g000002 Rmu_sc0008559.1_g000005 Rmu_sc0008560.1_g000002 Rmu_sc0008866.1_g000005 Rmu_sc0011028.1_g000006 Rmu_sc0011028.1_g000009 Rmu_sc0011346.1_g000003 Rmu_sc0011346.1_g000004 Rmu_sc0011346.1_g000012 Rmu_sc0011461.1_g000002 Rmu_sc0013048.1_g000001 Rmu_sc0015960.1_g000001 Rmu_sc0026214.1_g000001
rosa_roxburghii Rroxscaffold_1G00041310 Rroxscaffold_1G00041400 Rroxscaffold_1G00041410 Rroxscaffold_4G00301900 Rroxscaffold_4G00301910 Rroxscaffold_4G00301970 Rroxscaffold_4G00301990 Rroxscaffold_4G00302010 Rroxscaffold_4G00302030 Rroxscaffold_4G00303460 Rroxscaffold_6G00403270 Rroxscaffold_6G00403300 Rroxscaffold_6G00403320 Rroxscaffold_6G00403330 Rroxscaffold_6G00403370 Rroxscaffold_6G00403380 Rroxscaffold_6G00403410 Rroxscaffold_6G00403420 Rroxscaffold_6G00403430 Rroxscaffold_6G00403480 Rroxscaffold_6G00403540 Rroxscaffold_6G00406750 Rroxscaffold_6G00416600 Rroxscaffold_6G00416620 Rroxscaffold_6G00416630 Rroxscaffold_6G00416640 Rroxscaffold_6G00417470 Rroxscaffold_6G00417510 Rroxscaffold_6G00417810 Rroxscaffold_6G00417860 Rroxscaffold_6G00419140
rosa_rugosa Rorug01G0009600 Rorug01G0222400 Rorug01G0222700 Rorug01G0222900 Rorug01G0233900 Rorug01G0234100 Rorug01G0234200 Rorug01G0234400 Rorug01G0235200 Rorug03G0054200 Rorug03G0056600 Rorug03G0056700 Rorug03G0056800 Rorug03G0056900 Rorug03G0057200 Rorug03G0064100 Rorug03G0064100 Rorug03G0064200 Rorug03G0165200 Rorug03G0165300 Rorug03G0165300 Rorug03G0165500 Rorug03G0165500 Rorug03G0165600 Rorug03G0165700 Rorug03G0165900 Rorug03G0166000 Rorug03G0166200 Rorug03G0166300 Rorug03G0166400 Rorug03G0166500 Rorug03G0166700 Rorug04G0127000 Rorug05G0182500 Rorug05G0182900 Rorug05G0183000
rosa_samantha Rh1DG234000 Rh1DG243600 Rh1DG243700 Rh2AG645000 Rh3AG115000 Rh3BG118400 Rh3CG121200 Rh3CG121300 Rh3CG121700 Rh3CG121800 Rh3CG121900 Rh3CG128300 Rh3CG128500 Rh3CG243700 Rh3CG244200 Rh3CG244500 Rh3CG246000 Rh3CG246300 Rh3CG246400 Rh3CG246500 Rh3CG247000 Rh3DG120400 Rh4DG032600 Rh5AG319500 Rh5DG280900 Rh5DG337500 Rh6CG079500
rosa_wichuraiana Rw0G003790 Rw0G006960 Rw0G010120 Rw0G018070 Rw1G001290 Rw1G020570 Rw1G020590 Rw1G021380 Rw1G021390 Rw1G021440 Rw1G021540 Rw2G052980 Rw3G009520 Rw3G009740 Rw3G010350 Rw3G019410 Rw3G019560 Rw3G019590 Rw3G019600 Rw3G019610 Rw3G019620 Rw3G019630 Rw3G019650 Rw4G015840 Rw5G025130 Rw5G025840 Rw6G007960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 612, 850, 2005, 2929
AccBSI CCGCTC 1 cut(s) 2781
AccI GTMKAC 3 cut(s) 78, 473, 749
AccIII TCCGGA 1 cut(s) 760
AciI CCGC 5 cut(s) 416, 887, 1869, 1913, 2781
AclWI GGATC 6 cut(s) 195, 934, 1051, 2319, 2647, 2838
AcoI YGGCCR 2 cut(s) 82, 2375
AcuI CTGAAG 3 cut(s) 722, 1820, 2000
AcyI GRCGYC 1 cut(s) 2006
AfaI GTAC 5 cut(s) 314, 1362, 2097, 2713, 3334
AfiI CCNNNNNNNGG 5 cut(s) 322, 1203, 1837, 1922, 3137
AflII CTTAAG 2 cut(s) 707, 1436
AflIII ACRYGT 1 cut(s) 2435
AgeI ACCGGT 1 cut(s) 377
AjnI CCWGG 5 cut(s) 846, 2372, 2428, 2447, 2826
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
Alw26I GTCTC 5 cut(s) 302, 1523, 1553, 2655, 3225
AlwI GGATC 6 cut(s) 195, 934, 1051, 2319, 2647, 2838
Aor13HI TCCGGA 1 cut(s) 760
ApeKI GCWGC 7 cut(s) 112, 249, 370, 538, 889, 1294, 1498
AseI ATTAAT 3 cut(s) 1191, 1548, 2493
AsiGI ACCGGT 1 cut(s) 377
Asp700I GAANNNNTTC 2 cut(s) 842, 1106
AspLEI GCGC 1 cut(s) 2008
AspS9I GGNCC 9 cut(s) 680, 1138, 1400, 1672, 1778, 2369, 2758, 3266, 3312
AsuC2I CCSGG 2 cut(s) 93, 495
AsuHPI GGTGA 7 cut(s) 1237, 1709, 1937, 2432, 2489, 2596, 2883
AvaII GGWCC 4 cut(s) 1672, 1778, 2758, 3266
AxyI CCTNAGG 1 cut(s) 2649
BaeGI GKGCMC 1 cut(s) 2932
BalI TGGCCA 1 cut(s) 2377
BanI GGYRCC 4 cut(s) 612, 850, 2005, 2929
BbsI GAAGAC 4 cut(s) 1071, 2593, 3192, 3255
BbvI GCAGC 7 cut(s) 99, 261, 382, 525, 901, 1281, 1510
BceAI ACGGC 2 cut(s) 97, 196
BcgI CGANNNNNNTGC 2 cut(s) 2459, 2493
BciT130I CCWGG 5 cut(s) 848, 2374, 2430, 2449, 2828
BciVI GTATCC 1 cut(s) 2263
BcnI CCSGG 2 cut(s) 93, 495
BcoDI GTCTC 5 cut(s) 302, 1523, 1553, 2655, 3225
BfaI CTAG 5 cut(s) 1451, 1800, 1905, 2045, 2570
BfmI CTRYAG 5 cut(s) 860, 1295, 1304, 2516, 3223
BfoI RGCGCY 1 cut(s) 2009
BfrI CTTAAG 2 cut(s) 707, 1436
BfuI GTATCC 1 cut(s) 2263
BglII AGATCT 2 cut(s) 840, 2589
BisI GCNGC 8 cut(s) 113, 250, 371, 539, 890, 1295, 1499, 1869
BlsI GCNGC 8 cut(s) 114, 251, 372, 540, 891, 1296, 1500, 1870
BmcAI AGTACT 1 cut(s) 2097
Bme1390I CCNGG 7 cut(s) 93, 495, 848, 2374, 2430, 2449, 2828
Bme18I GGWCC 4 cut(s) 1672, 1778, 2758, 3266
BmgT120I GGNCC 9 cut(s) 680, 1138, 1400, 1672, 1778, 2369, 2758, 3266, 3312
BmiI GGNNCC 9 cut(s) 426, 614, 852, 901, 995, 2007, 2371, 2931, 3267
BmrFI CCNGG 7 cut(s) 93, 495, 848, 2374, 2430, 2449, 2828
BmrI ACTGGG 1 cut(s) 2738
BmsI GCATC 7 cut(s) 69, 963, 1281, 1339, 2054, 2467, 2533
BmuI ACTGGG 1 cut(s) 2738
BpiI GAAGAC 4 cut(s) 1071, 2593, 3192, 3255
BplI GAGNNNNNCTC 2 cut(s) 755, 787
BpmI CTGGAG 2 cut(s) 2870, 3039
Bpu10I CCTNAGC 1 cut(s) 542
BpuEI CTTGAG 7 cut(s) 265, 284, 536, 664, 1663, 1870, 2768
BpuMI CCSGG 2 cut(s) 93, 495
BsaBI GATNNNNATC 1 cut(s) 2615
BsaHI GRCGYC 1 cut(s) 2006
BsaI GGTCTC 3 cut(s) 302, 1553, 2655
BsaJI CCNNGG 6 cut(s) 493, 1429, 1975, 2372, 2481, 2754
BsaWI WCCGGW 3 cut(s) 377, 760, 878
BsaXI ACNNNNNCTCC 6 cut(s) 1711, 1741, 3030, 3036, 3060, 3066
Bsc4I CCNNNNNNNGG 5 cut(s) 322, 1203, 1837, 1922, 3137
Bse118I RCCGGY 1 cut(s) 377
Bse21I CCTNAGG 1 cut(s) 2649
Bse3DI GCAATG 2 cut(s) 632, 2463
Bse8I GATNNNNATC 1 cut(s) 2615
BseAI TCCGGA 1 cut(s) 760
BseBI CCWGG 5 cut(s) 848, 2374, 2430, 2449, 2828
BseDI CCNNGG 6 cut(s) 493, 1429, 1975, 2372, 2481, 2754
BseGI GGATG 4 cut(s) 538, 2357, 2548, 3315
BseJI GATNNNNATC 1 cut(s) 2615
BseLI CCNNNNNNNGG 5 cut(s) 322, 1203, 1837, 1922, 3137
BseMI GCAATG 2 cut(s) 632, 2463
BseRI GAGGAG 2 cut(s) 3191, 3194
BseSI GKGCMC 1 cut(s) 2932
BseXI GCAGC 7 cut(s) 99, 261, 382, 525, 901, 1281, 1510
BseYI CCCAGC 1 cut(s) 1203
BsgI GTGCAG 1 cut(s) 2209
Bsh1285I CGRYCG 1 cut(s) 938
BshNI GGYRCC 4 cut(s) 612, 850, 2005, 2929
BshTI ACCGGT 1 cut(s) 377
BsiEI CGRYCG 1 cut(s) 938
BsiSI CCGG 5 cut(s) 93, 378, 495, 761, 879
BslFI GGGAC 6 cut(s) 75, 616, 1273, 1854, 2117, 3182
BslI CCNNNNNNNGG 5 cut(s) 322, 1203, 1837, 1922, 3137
BsmAI GTCTC 5 cut(s) 302, 1523, 1553, 2655, 3225
BsmBI CGTCTC 1 cut(s) 3225
BsmFI GGGAC 6 cut(s) 75, 616, 1273, 1854, 2117, 3182
BsmI GAATGC 3 cut(s) 349, 409, 1908
Bso31I GGTCTC 3 cut(s) 302, 1553, 2655
Bsp1286I GDGCHC 1 cut(s) 2932
Bsp13I TCCGGA 1 cut(s) 760
Bsp1407I TGTACA 1 cut(s) 3332
Bsp19I CCATGG 1 cut(s) 2481
BspACI CCGC 5 cut(s) 416, 887, 1869, 1913, 2781
BspEI TCCGGA 1 cut(s) 760
BspHI TCATGA 1 cut(s) 804
BspLI GGNNCC 9 cut(s) 426, 614, 852, 901, 995, 2007, 2371, 2931, 3267
BspMAI CTGCAG 3 cut(s) 864, 1299, 2520
BspPI GGATC 6 cut(s) 195, 934, 1051, 2319, 2647, 2838
BspT107I GGYRCC 4 cut(s) 612, 850, 2005, 2929
BspTI CTTAAG 2 cut(s) 707, 1436
BspTNI GGTCTC 3 cut(s) 302, 1553, 2655
BsrBI CCGCTC 1 cut(s) 2781
BsrDI GCAATG 2 cut(s) 632, 2463
BsrFI RCCGGY 1 cut(s) 377
BsrGI TGTACA 1 cut(s) 3332
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 6 cut(s) 493, 1429, 1975, 2372, 2481, 2754
BssNI GRCGYC 1 cut(s) 2006
BssT1I CCWWGG 2 cut(s) 2481, 2754
Bst2UI CCWGG 5 cut(s) 848, 2374, 2430, 2449, 2828
Bst4CI ACNGT 6 cut(s) 50, 1077, 1945, 2554, 2599, 3332
Bst6I CTCTTC 4 cut(s) 936, 3192, 3302, 3379
BstACI GRCGYC 1 cut(s) 2006
BstAFI CTTAAG 2 cut(s) 707, 1436
BstAPI GCANNNNNTGC 1 cut(s) 109
BstAUI TGTACA 1 cut(s) 3332
BstC8I GCNNGC 3 cut(s) 409, 1615, 2404
BstDSI CCRYGG 2 cut(s) 1975, 2481
BstEII GGTNACC 2 cut(s) 1765, 2732
BstF5I GGATG 4 cut(s) 538, 2357, 2548, 3315
BstH2I RGCGCY 1 cut(s) 2009
BstHHI GCGC 1 cut(s) 2008
BstMAI GTCTC 5 cut(s) 302, 1523, 1553, 2655, 3225
BstMCI CGRYCG 1 cut(s) 938
BstMWI GCNNNNNNNGC 6 cut(s) 109, 413, 712, 859, 1300, 2462
BstNI CCWGG 5 cut(s) 848, 2374, 2430, 2449, 2828
BstNSI RCATGY 3 cut(s) 2439, 2949, 3339
BstPI GGTNACC 2 cut(s) 1765, 2732
BstSCI CCNGG 7 cut(s) 91, 493, 846, 2372, 2428, 2447, 2826
BstSFI CTRYAG 5 cut(s) 860, 1295, 1304, 2516, 3223
BstSLI GKGCMC 1 cut(s) 2932
BstV1I GCAGC 7 cut(s) 99, 261, 382, 525, 901, 1281, 1510
BstV2I GAAGAC 4 cut(s) 1071, 2593, 3192, 3255
BstX2I RGATCY 4 cut(s) 840, 926, 2589, 2830
BstXI CCANNNNNNTGG 2 cut(s) 1694, 2339
BstYI RGATCY 4 cut(s) 840, 926, 2589, 2830
Bsu36I CCTNAGG 1 cut(s) 2649
BsuI GTATCC 1 cut(s) 2263
BtgI CCRYGG 2 cut(s) 1975, 2481
BtsCI GGATG 4 cut(s) 538, 2357, 2548, 3315
BtsI GCAGTG 4 cut(s) 144, 190, 857, 2956
Cac8I GCNNGC 3 cut(s) 409, 1615, 2404
CciI TCATGA 1 cut(s) 804
CfoI GCGC 1 cut(s) 2008
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 9 cut(s) 680, 1138, 1400, 1672, 1778, 2369, 2758, 3266, 3312
CseI GACGC 1 cut(s) 1251
CsiI ACCWGGT 1 cut(s) 2447
Csp6I GTAC 5 cut(s) 313, 1361, 2096, 2712, 3333
CspAI ACCGGT 1 cut(s) 377
CspCI CAANNNNNGTGG 2 cut(s) 2398, 2433
CviQI GTAC 5 cut(s) 313, 1361, 2096, 2712, 3333
DinI GGCGCC 1 cut(s) 2007
DraI TTTAAA 1 cut(s) 2163
EaeI YGGCCR 2 cut(s) 82, 2375
Eam1104I CTCTTC 4 cut(s) 936, 3192, 3302, 3379
EarI CTCTTC 4 cut(s) 936, 3192, 3302, 3379
EciI GGCGGA 1 cut(s) 1902
Eco130I CCWWGG 2 cut(s) 2481, 2754
Eco147I AGGCCT 1 cut(s) 2199
Eco31I GGTCTC 3 cut(s) 302, 1553, 2655
Eco32I GATATC 2 cut(s) 624, 2193
Eco47I GGWCC 4 cut(s) 1672, 1778, 2758, 3266
Eco57I CTGAAG 3 cut(s) 722, 1820, 2000
Eco81I CCTNAGG 1 cut(s) 2649
Eco91I GGTNACC 2 cut(s) 1765, 2732
EcoO109I RGGNCCY 2 cut(s) 680, 1778
EcoO65I GGTNACC 2 cut(s) 1765, 2732
EcoRI GAATTC 2 cut(s) 808, 2859
EcoRII CCWGG 5 cut(s) 846, 2372, 2428, 2447, 2826
EcoRV GATATC 2 cut(s) 624, 2193
EcoT14I CCWWGG 2 cut(s) 2481, 2754
EgeI GGCGCC 1 cut(s) 2007
EheI GGCGCC 1 cut(s) 2007
ErhI CCWWGG 2 cut(s) 2481, 2754
Esp3I CGTCTC 1 cut(s) 3225
FalI AAGNNNNNCTT 2 cut(s) 827, 859
FaqI GGGAC 6 cut(s) 75, 616, 1273, 1854, 2117, 3182
FauI CCCGC 1 cut(s) 409
FblI GTMKAC 3 cut(s) 78, 473, 749
Fnu4HI GCNGC 8 cut(s) 113, 250, 371, 539, 890, 1295, 1499, 1869
FokI GGATG 4 cut(s) 545, 2364, 2555, 3302
Fsp4HI GCNGC 8 cut(s) 113, 250, 371, 539, 890, 1295, 1499, 1869
FspBI CTAG 5 cut(s) 1451, 1800, 1905, 2045, 2570
GlaI GCGC 1 cut(s) 2007
GluI GCNGC 8 cut(s) 113, 250, 371, 539, 890, 1295, 1499, 1869
GsaI CCCAGC 1 cut(s) 1207
GsuI CTGGAG 2 cut(s) 2870, 3039
HaeII RGCGCY 1 cut(s) 2009
HapII CCGG 5 cut(s) 93, 378, 495, 761, 879
HgaI GACGC 1 cut(s) 1251
HhaI GCGC 1 cut(s) 2008
Hin1I GRCGYC 1 cut(s) 2006
Hin6I GCGC 1 cut(s) 2006
HinP1I GCGC 1 cut(s) 2006
HincII GTYRAC 2 cut(s) 1179, 2395
HindII GTYRAC 2 cut(s) 1179, 2395
HindIII AAGCTT 2 cut(s) 704, 2381
HpaI GTTAAC 1 cut(s) 1179
HpaII CCGG 5 cut(s) 93, 378, 495, 761, 879
HphI GGTGA 7 cut(s) 1237, 1709, 1937, 2432, 2489, 2596, 2883
Hpy166II GTNNAC 7 cut(s) 79, 269, 474, 663, 750, 1179, 2395
Hpy8I GTNNAC 7 cut(s) 79, 269, 474, 663, 750, 1179, 2395
Hpy99I CGWCG 2 cut(s) 508, 1245
HpyAV CCTTC 5 cut(s) 197, 1391, 1791, 1829, 3071
HpyCH4III ACNGT 6 cut(s) 50, 1077, 1945, 2554, 2599, 3332
HpyCH4IV ACGT 1 cut(s) 66
HpyF10VI GCNNNNNNNGC 6 cut(s) 109, 413, 712, 859, 1300, 2462
HpySE526I ACGT 1 cut(s) 66
Hsp92I GRCGYC 1 cut(s) 2006
HspAI GCGC 1 cut(s) 2006
KasI GGCGCC 1 cut(s) 2005
Kpn2I TCCGGA 1 cut(s) 760
KspAI GTTAAC 1 cut(s) 1179
LmnI GCTCC 1 cut(s) 3038
Lsp1109I GCAGC 7 cut(s) 99, 261, 382, 525, 901, 1281, 1510
LweI GCATC 7 cut(s) 69, 963, 1281, 1339, 2054, 2467, 2533
MabI ACCWGGT 1 cut(s) 2447
MaeI CTAG 5 cut(s) 1451, 1800, 1905, 2045, 2570
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 6 cut(s) 457, 668, 1765, 2354, 2554, 2732
MbiI CCGCTC 1 cut(s) 2781
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 4 cut(s) 840, 926, 2589, 2830
MhlI GDGCHC 1 cut(s) 2932
MlsI TGGCCA 1 cut(s) 2377
MluNI TGGCCA 1 cut(s) 2377
Mly113I GGCGCC 1 cut(s) 2006
MlyI GAGTC 4 cut(s) 244, 1525, 2759, 2947
MmeI TCCRAC 4 cut(s) 1374, 1851, 2352, 2888
Mox20I TGGCCA 1 cut(s) 2377
MroI TCCGGA 1 cut(s) 760
MroXI GAANNNNTTC 2 cut(s) 842, 1106
MscI TGGCCA 1 cut(s) 2377
MslI CAYNNNNRTG 1 cut(s) 2337
Msp20I TGGCCA 1 cut(s) 2377
MspCI CTTAAG 2 cut(s) 707, 1436
MspI CCGG 5 cut(s) 93, 378, 495, 761, 879
MspR9I CCNGG 7 cut(s) 93, 495, 848, 2374, 2430, 2449, 2828
MunI CAATTG 1 cut(s) 119
Mva1269I GAATGC 3 cut(s) 349, 409, 1908
MvaI CCWGG 5 cut(s) 848, 2374, 2430, 2449, 2828
MwoI GCNNNNNNNGC 6 cut(s) 109, 413, 712, 859, 1300, 2462
NarI GGCGCC 1 cut(s) 2006
NciI CCSGG 2 cut(s) 93, 495
NcoI CCATGG 1 cut(s) 2481
NlaIV GGNNCC 9 cut(s) 426, 614, 852, 901, 995, 2007, 2371, 2931, 3267
NspI RCATGY 3 cut(s) 2439, 2949, 3339
PagI TCATGA 1 cut(s) 804
PceI AGGCCT 1 cut(s) 2199
PciI ACATGT 1 cut(s) 2435
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 779
PctI GAATGC 3 cut(s) 349, 409, 1908
PdmI GAANNNNTTC 2 cut(s) 842, 1106
PfoI TCCNGGA 1 cut(s) 2826
PinAI ACCGGT 1 cut(s) 377
PkrI GCNGC 8 cut(s) 114, 251, 372, 540, 891, 1296, 1500, 1870
Ple19I CGATCG 1 cut(s) 938
PleI GAGTC 4 cut(s) 243, 1524, 2758, 2946
PluTI GGCGCC 1 cut(s) 2009
PpsI GAGTC 4 cut(s) 243, 1524, 2758, 2946
PpuMI RGGWCCY 1 cut(s) 1778
PscI ACATGT 1 cut(s) 2435
PshBI ATTAAT 3 cut(s) 1191, 1548, 2493
Psp5II RGGWCCY 1 cut(s) 1778
Psp6I CCWGG 5 cut(s) 846, 2372, 2428, 2447, 2826
PspEI GGTNACC 2 cut(s) 1765, 2732
PspFI CCCAGC 1 cut(s) 1203
PspGI CCWGG 5 cut(s) 846, 2372, 2428, 2447, 2826
PspN4I GGNNCC 9 cut(s) 426, 614, 852, 901, 995, 2007, 2371, 2931, 3267
PspPI GGNCC 9 cut(s) 680, 1138, 1400, 1672, 1778, 2369, 2758, 3266, 3312
PspPPI RGGWCCY 1 cut(s) 1778
PstI CTGCAG 3 cut(s) 864, 1299, 2520
PsuI RGATCY 4 cut(s) 840, 926, 2589, 2830
PvuI CGATCG 1 cut(s) 938
RsaI GTAC 5 cut(s) 314, 1362, 2097, 2713, 3334
RsaNI GTAC 5 cut(s) 313, 1361, 2096, 2712, 3333
RseI CAYNNNNRTG 1 cut(s) 2337
SatI GCNGC 8 cut(s) 113, 250, 371, 539, 890, 1295, 1499, 1869
Sau96I GGNCC 9 cut(s) 680, 1138, 1400, 1672, 1778, 2369, 2758, 3266, 3312
ScaI AGTACT 1 cut(s) 2097
SchI GAGTC 4 cut(s) 244, 1525, 2759, 2947
ScrFI CCNGG 7 cut(s) 93, 495, 848, 2374, 2430, 2449, 2828
SduI GDGCHC 1 cut(s) 2932
SexAI ACCWGGT 1 cut(s) 2447
SfaNI GCATC 7 cut(s) 69, 963, 1281, 1339, 2054, 2467, 2533
SfcI CTRYAG 5 cut(s) 860, 1295, 1304, 2516, 3223
SfoI GGCGCC 1 cut(s) 2007
SinI GGWCC 4 cut(s) 1672, 1778, 2758, 3266
SmiMI CAYNNNNRTG 1 cut(s) 2337
SmlI CTYRAG 9 cut(s) 244, 299, 515, 643, 707, 1436, 1642, 1885, 2747
SmoI CTYRAG 9 cut(s) 244, 299, 515, 643, 707, 1436, 1642, 1885, 2747
SseBI AGGCCT 1 cut(s) 2199
SsiI CCGC 5 cut(s) 416, 887, 1869, 1913, 2781
SspDI GGCGCC 1 cut(s) 2005
SspI AATATT 1 cut(s) 1591
SspMI CTAG 5 cut(s) 1451, 1800, 1905, 2045, 2570
StuI AGGCCT 1 cut(s) 2199
StyD4I CCNGG 7 cut(s) 91, 493, 846, 2372, 2428, 2447, 2826
StyI CCWWGG 2 cut(s) 2481, 2754
TaaI ACNGT 6 cut(s) 50, 1077, 1945, 2554, 2599, 3332
TaiI ACGT 1 cut(s) 69
TaqII GACCGA 1 cut(s) 284
TatI WGTACW 3 cut(s) 1360, 2095, 3332
TauI GCSGC 1 cut(s) 1871
TseI GCWGC 7 cut(s) 112, 249, 370, 538, 889, 1294, 1498
TspGWI ACGGA 2 cut(s) 492, 939
Vha464I CTTAAG 2 cut(s) 707, 1436
VpaK11BI GGWCC 4 cut(s) 1672, 1778, 2758, 3266
VspI ATTAAT 3 cut(s) 1191, 1548, 2493
XbaI TCTAGA 1 cut(s) 2569
XceI RCATGY 3 cut(s) 2439, 2949, 3339
XmiI GTMKAC 3 cut(s) 78, 473, 749
XmnI GAANNNNTTC 2 cut(s) 842, 1106
XspI CTAG 5 cut(s) 1451, 1800, 1905, 2045, 2570
ZrmI AGTACT 1 cut(s) 2097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.