Leucine rich repeat N-terminal domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1542, 1574 |
| Acc36I | ACCTGC | 1 cut(s) | 615 |
| AccBSI | CCGCTC | 1 cut(s) | 1326 |
| AccI | GTMKAC | 1 cut(s) | 350 |
| AciI | CCGC | 1 cut(s) | 1326 |
| AclWI | GGATC | 3 cut(s) | 530, 1192, 1399 |
| AcoI | YGGCCR | 1 cut(s) | 917 |
| AcsI | RAATTY | 5 cut(s) | 743, 875, 1046, 1175, 1332 |
| AcuI | CTGAAG | 1 cut(s) | 40 |
| AfaI | GTAC | 2 cut(s) | 639, 814 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 379, 1327, 1484, 1532, 1609, 1710 |
| AflII | CTTAAG | 1 cut(s) | 586 |
| AflIII | ACRYGT | 1 cut(s) | 1288 |
| AjnI | CCWGG | 2 cut(s) | 238, 989 |
| AluBI | AGCT | 7 cut(s) | 444, 915, 944, 1126, 1345, 1354, 1442 |
| AluI | AGCT | 7 cut(s) | 444, 915, 944, 1126, 1345, 1354, 1442 |
| AlwI | GGATC | 3 cut(s) | 530, 1192, 1399 |
| AoxI | GGCC | 6 cut(s) | 408, 917, 1472, 1600, 1611, 1630 |
| ApeKI | GCWGC | 1 cut(s) | 912 |
| ApoI | RAATTY | 5 cut(s) | 743, 875, 1046, 1175, 1332 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1038, 1070 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1175, 1687 |
| AspLEI | GCGC | 1 cut(s) | 888 |
| AspS9I | GGNCC | 7 cut(s) | 217, 323, 408, 1054, 1296, 1472, 1630 |
| AsuC2I | CCSGG | 1 cut(s) | 1168 |
| AsuHPI | GGTGA | 6 cut(s) | 254, 479, 1141, 1141, 1171, 1268 |
| AsuNHI | GCTAGC | 1 cut(s) | 1019 |
| AvaII | GGWCC | 4 cut(s) | 217, 323, 1054, 1296 |
| AxyI | CCTNAGG | 1 cut(s) | 1498 |
| BalI | TGGCCA | 1 cut(s) | 919 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 312, 344 |
| BbsI | GAAGAC | 1 cut(s) | 147 |
| BbvI | GCAGC | 1 cut(s) | 924 |
| BccI | CCATC | 7 cut(s) | 14, 399, 997, 1114, 1273, 1345, 1646 |
| BciT130I | CCWGG | 2 cut(s) | 240, 991 |
| BciVI | GTATCC | 1 cut(s) | 1707 |
| BcnI | CCSGG | 1 cut(s) | 1168 |
| BfaI | CTAG | 7 cut(s) | 317, 713, 776, 941, 1020, 1115, 1239 |
| BfrI | CTTAAG | 1 cut(s) | 586 |
| BfuAI | ACCTGC | 1 cut(s) | 615 |
| BfuI | GTATCC | 1 cut(s) | 1707 |
| BisI | GCNGC | 1 cut(s) | 913 |
| BlsI | GCNGC | 1 cut(s) | 914 |
| BmcAI | AGTACT | 1 cut(s) | 639 |
| Bme1390I | CCNGG | 3 cut(s) | 240, 991, 1168 |
| Bme18I | GGWCC | 4 cut(s) | 217, 323, 1054, 1296 |
| BmgT120I | GGNCC | 7 cut(s) | 217, 323, 408, 1054, 1296, 1472, 1630 |
| BmiI | GGNNCC | 2 cut(s) | 1297, 1473 |
| BmrFI | CCNGG | 3 cut(s) | 240, 991, 1168 |
| BmrI | ACTGGG | 1 cut(s) | 598 |
| BmsI | GCATC | 2 cut(s) | 968, 1006 |
| BmtI | GCTAGC | 1 cut(s) | 1023 |
| BmuI | ACTGGG | 1 cut(s) | 598 |
| BpiI | GAAGAC | 1 cut(s) | 147 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1322 |
| BpuEI | CTTGAG | 3 cut(s) | 208, 412, 430 |
| BpuMI | CCSGG | 1 cut(s) | 1168 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1566 |
| BsaJI | CCNNGG | 4 cut(s) | 517, 790, 1299, 1614 |
| BsaWI | WCCGGW | 2 cut(s) | 1181, 1397 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 379, 1327, 1484, 1532, 1609, 1710 |
| Bse1I | ACTGG | 5 cut(s) | 260, 373, 604, 670, 1038 |
| Bse21I | CCTNAGG | 1 cut(s) | 1498 |
| Bse3DI | GCAATG | 1 cut(s) | 1462 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1566 |
| BseBI | CCWGG | 2 cut(s) | 240, 991 |
| BseDI | CCNNGG | 4 cut(s) | 517, 790, 1299, 1614 |
| BseGI | GGATG | 1 cut(s) | 899 |
| BseJI | GATNNNNATC | 1 cut(s) | 1566 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 379, 1327, 1484, 1532, 1609, 1710 |
| BseMI | GCAATG | 1 cut(s) | 1462 |
| BseMII | CTCAG | 5 cut(s) | 1085, 1313, 1433, 1512, 1572 |
| BseNI | ACTGG | 5 cut(s) | 260, 373, 604, 670, 1038 |
| BseRI | GAGGAG | 1 cut(s) | 333 |
| BseXI | GCAGC | 1 cut(s) | 924 |
| BshFI | GGCC | 6 cut(s) | 410, 919, 1474, 1602, 1613, 1632 |
| BsiSI | CCGG | 4 cut(s) | 455, 1167, 1182, 1398 |
| BslFI | GGGAC | 1 cut(s) | 396 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 379, 1327, 1484, 1532, 1609, 1710 |
| BsmFI | GGGAC | 1 cut(s) | 396 |
| BsmI | GAATGC | 3 cut(s) | 450, 1367, 1621 |
| BsnI | GGCC | 6 cut(s) | 410, 919, 1474, 1602, 1613, 1632 |
| Bsp143I | GATC | 7 cut(s) | 522, 568, 804, 866, 1184, 1404, 1567 |
| Bsp19I | CCATGG | 1 cut(s) | 517 |
| BspACI | CCGC | 1 cut(s) | 1326 |
| BspANI | GGCC | 6 cut(s) | 410, 919, 1474, 1602, 1613, 1632 |
| BspCNI | CTCAG | 5 cut(s) | 1084, 1314, 1432, 1511, 1573 |
| BspLI | GGNNCC | 2 cut(s) | 1297, 1473 |
| BspMI | ACCTGC | 1 cut(s) | 615 |
| BspOI | GCTAGC | 1 cut(s) | 1023 |
| BspPI | GGATC | 3 cut(s) | 530, 1192, 1399 |
| BspTI | CTTAAG | 1 cut(s) | 586 |
| BsrBI | CCGCTC | 1 cut(s) | 1326 |
| BsrDI | GCAATG | 1 cut(s) | 1462 |
| BsrI | ACTGG | 5 cut(s) | 260, 373, 604, 670, 1038 |
| BssECI | CCNNGG | 4 cut(s) | 517, 790, 1299, 1614 |
| BssMI | GATC | 7 cut(s) | 522, 568, 804, 866, 1184, 1404, 1567 |
| BssT1I | CCWWGG | 4 cut(s) | 517, 790, 1299, 1614 |
| Bst2UI | CCWGG | 2 cut(s) | 240, 991 |
| Bst4CI | ACNGT | 1 cut(s) | 1677 |
| BstAFI | CTTAAG | 1 cut(s) | 586 |
| BstC8I | GCNNGC | 4 cut(s) | 917, 996, 1021, 1453 |
| BstDEI | CTNAG | 7 cut(s) | 307, 900, 1071, 1322, 1419, 1498, 1581 |
| BstDSI | CCRYGG | 1 cut(s) | 517 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1482 |
| BstF5I | GGATG | 1 cut(s) | 899 |
| BstHHI | GCGC | 1 cut(s) | 888 |
| BstKTI | GATC | 7 cut(s) | 525, 571, 807, 869, 1187, 1407, 1570 |
| BstMBI | GATC | 7 cut(s) | 522, 568, 804, 866, 1184, 1404, 1567 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 847, 1004, 1351, 1448 |
| BstNI | CCWGG | 2 cut(s) | 240, 991 |
| BstNSI | RCATGY | 2 cut(s) | 854, 1292 |
| BstSCI | CCNGG | 3 cut(s) | 238, 989, 1166 |
| BstV1I | GCAGC | 1 cut(s) | 924 |
| BstV2I | GAAGAC | 1 cut(s) | 147 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 239, 418 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1498 |
| BsuI | GTATCC | 1 cut(s) | 1707 |
| BsuRI | GGCC | 6 cut(s) | 410, 919, 1474, 1602, 1613, 1632 |
| BtgI | CCRYGG | 1 cut(s) | 517 |
| BtgZI | GCGATG | 2 cut(s) | 1013, 1665 |
| BtsCI | GGATG | 1 cut(s) | 899 |
| BtsIMutI | CAGTG | 4 cut(s) | 64, 611, 814, 964 |
| BveI | ACCTGC | 1 cut(s) | 615 |
| Cac8I | GCNNGC | 4 cut(s) | 917, 996, 1021, 1453 |
| CfoI | GCGC | 1 cut(s) | 888 |
| Cfr13I | GGNCC | 7 cut(s) | 217, 323, 408, 1054, 1296, 1472, 1630 |
| Csp6I | GTAC | 2 cut(s) | 638, 813 |
| CspCI | CAANNNNNGTGG | 6 cut(s) | 40, 75, 940, 975, 1176, 1211 |
| CviAII | CATG | 4 cut(s) | 297, 518, 851, 1289 |
| CviQI | GTAC | 2 cut(s) | 638, 813 |
| DdeI | CTNAG | 7 cut(s) | 307, 900, 1071, 1322, 1419, 1498, 1581 |
| DpnI | GATC | 7 cut(s) | 524, 570, 806, 868, 1186, 1406, 1569 |
| DpnII | GATC | 7 cut(s) | 522, 568, 804, 866, 1184, 1404, 1567 |
| EaeI | YGGCCR | 1 cut(s) | 917 |
| Eco130I | CCWWGG | 4 cut(s) | 517, 790, 1299, 1614 |
| Eco32I | GATATC | 1 cut(s) | 463 |
| Eco47I | GGWCC | 4 cut(s) | 217, 323, 1054, 1296 |
| Eco57I | CTGAAG | 1 cut(s) | 40 |
| Eco81I | CCTNAGG | 1 cut(s) | 1498 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1482 |
| EcoO109I | RGGNCCY | 4 cut(s) | 323, 1054, 1296, 1472 |
| EcoRII | CCWGG | 2 cut(s) | 238, 989 |
| EcoRV | GATATC | 1 cut(s) | 463 |
| EcoT14I | CCWWGG | 4 cut(s) | 517, 790, 1299, 1614 |
| ErhI | CCWWGG | 4 cut(s) | 517, 790, 1299, 1614 |
| FaeI | CATG | 4 cut(s) | 300, 521, 854, 1292 |
| FaqI | GGGAC | 1 cut(s) | 396 |
| FatI | CATG | 4 cut(s) | 296, 517, 850, 1288 |
| FauI | CCCGC | 1 cut(s) | 1319 |
| FblI | GTMKAC | 1 cut(s) | 350 |
| Fnu4HI | GCNGC | 1 cut(s) | 913 |
| FokI | GGATG | 1 cut(s) | 906 |
| Fsp4HI | GCNGC | 1 cut(s) | 913 |
| FspBI | CTAG | 7 cut(s) | 317, 713, 776, 941, 1020, 1115, 1239 |
| GlaI | GCGC | 1 cut(s) | 887 |
| GluI | GCNGC | 1 cut(s) | 913 |
| HaeIII | GGCC | 6 cut(s) | 410, 919, 1474, 1602, 1613, 1632 |
| HapII | CCGG | 4 cut(s) | 455, 1167, 1182, 1398 |
| HhaI | GCGC | 1 cut(s) | 888 |
| Hin1II | CATG | 4 cut(s) | 300, 521, 854, 1292 |
| Hin6I | GCGC | 1 cut(s) | 886 |
| HinP1I | GCGC | 1 cut(s) | 886 |
| HinfI | GANTC | 5 cut(s) | 235, 533, 598, 752, 1375 |
| HpaII | CCGG | 4 cut(s) | 455, 1167, 1182, 1398 |
| HphI | GGTGA | 6 cut(s) | 254, 479, 1141, 1141, 1171, 1268 |
| Hpy166II | GTNNAC | 1 cut(s) | 351 |
| Hpy188I | TCNGA | 2 cut(s) | 804, 1074 |
| Hpy8I | GTNNAC | 1 cut(s) | 351 |
| Hpy99I | CGWCG | 1 cut(s) | 1088 |
| HpyAV | CCTTC | 3 cut(s) | 314, 371, 1454 |
| HpyCH4III | ACNGT | 1 cut(s) | 1677 |
| HpyCH4IV | ACGT | 1 cut(s) | 1358 |
| HpyCH4V | TGCA | 6 cut(s) | 300, 617, 768, 850, 1367, 1451 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 847, 1004, 1351, 1448 |
| HpyF3I | CTNAG | 7 cut(s) | 307, 900, 1071, 1322, 1419, 1498, 1581 |
| HpySE526I | ACGT | 1 cut(s) | 1358 |
| Hsp92II | CATG | 4 cut(s) | 300, 521, 854, 1292 |
| HspAI | GCGC | 1 cut(s) | 886 |
| Kzo9I | GATC | 7 cut(s) | 522, 568, 804, 866, 1184, 1404, 1567 |
| LmnI | GCTCC | 3 cut(s) | 621, 1123, 1618 |
| Lsp1109I | GCAGC | 1 cut(s) | 924 |
| LweI | GCATC | 2 cut(s) | 968, 1006 |
| MaeI | CTAG | 7 cut(s) | 317, 713, 776, 941, 1020, 1115, 1239 |
| MaeII | ACGT | 1 cut(s) | 1358 |
| MaeIII | GTNAC | 3 cut(s) | 310, 1026, 1099 |
| MalI | GATC | 7 cut(s) | 524, 570, 806, 868, 1186, 1406, 1569 |
| MbiI | CCGCTC | 1 cut(s) | 1326 |
| MboI | GATC | 7 cut(s) | 522, 568, 804, 866, 1184, 1404, 1567 |
| MfeI | CAATTG | 3 cut(s) | 630, 763, 1155 |
| MlsI | TGGCCA | 1 cut(s) | 919 |
| MluNI | TGGCCA | 1 cut(s) | 919 |
| MmeI | TCCRAC | 2 cut(s) | 393, 1460 |
| Mox20I | TGGCCA | 1 cut(s) | 919 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1175, 1687 |
| MscI | TGGCCA | 1 cut(s) | 919 |
| MseI | TTAA | 8 cut(s) | 12, 227, 587, 611, 642, 647, 830, 1139 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1542 |
| Msp20I | TGGCCA | 1 cut(s) | 919 |
| MspA1I | CMGCKG | 1 cut(s) | 915 |
| MspCI | CTTAAG | 1 cut(s) | 586 |
| MspI | CCGG | 4 cut(s) | 455, 1167, 1182, 1398 |
| MspR9I | CCNGG | 3 cut(s) | 240, 991, 1168 |
| MunI | CAATTG | 3 cut(s) | 630, 763, 1155 |
| Mva1269I | GAATGC | 3 cut(s) | 450, 1367, 1621 |
| MvaI | CCWGG | 2 cut(s) | 240, 991 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 847, 1004, 1351, 1448 |
| NciI | CCSGG | 1 cut(s) | 1168 |
| NcoI | CCATGG | 1 cut(s) | 517 |
| NdeII | GATC | 7 cut(s) | 522, 568, 804, 866, 1184, 1404, 1567 |
| NheI | GCTAGC | 1 cut(s) | 1019 |
| NlaIII | CATG | 4 cut(s) | 300, 521, 854, 1292 |
| NlaIV | GGNNCC | 2 cut(s) | 1297, 1473 |
| NmuCI | GTSAC | 1 cut(s) | 1026 |
| NspI | RCATGY | 2 cut(s) | 854, 1292 |
| PciI | ACATGT | 1 cut(s) | 1288 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1634 |
| PctI | GAATGC | 3 cut(s) | 450, 1367, 1621 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1175, 1687 |
| PfeI | GAWTC | 5 cut(s) | 235, 533, 598, 752, 1375 |
| PfoI | TCCNGGA | 1 cut(s) | 1166 |
| PkrI | GCNGC | 1 cut(s) | 914 |
| PpuMI | RGGWCCY | 3 cut(s) | 323, 1054, 1296 |
| PscI | ACATGT | 1 cut(s) | 1288 |
| PsiI | TTATAA | 2 cut(s) | 1542, 1574 |
| Psp5II | RGGWCCY | 3 cut(s) | 323, 1054, 1296 |
| Psp6I | CCWGG | 2 cut(s) | 238, 989 |
| PspGI | CCWGG | 2 cut(s) | 238, 989 |
| PspN4I | GGNNCC | 2 cut(s) | 1297, 1473 |
| PspPI | GGNCC | 7 cut(s) | 217, 323, 408, 1054, 1296, 1472, 1630 |
| PspPPI | RGGWCCY | 3 cut(s) | 323, 1054, 1296 |
| PvuII | CAGCTG | 1 cut(s) | 915 |
| RsaI | GTAC | 2 cut(s) | 639, 814 |
| RsaNI | GTAC | 2 cut(s) | 638, 813 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1542 |
| SaqAI | TTAA | 8 cut(s) | 12, 227, 587, 611, 642, 647, 830, 1139 |
| SatI | GCNGC | 1 cut(s) | 913 |
| Sau3AI | GATC | 7 cut(s) | 522, 568, 804, 866, 1184, 1404, 1567 |
| Sau96I | GGNCC | 7 cut(s) | 217, 323, 408, 1054, 1296, 1472, 1630 |
| ScaI | AGTACT | 1 cut(s) | 639 |
| ScrFI | CCNGG | 3 cut(s) | 240, 991, 1168 |
| SfaNI | GCATC | 2 cut(s) | 968, 1006 |
| SinI | GGWCC | 4 cut(s) | 217, 323, 1054, 1296 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1542 |
| SmlI | CTYRAG | 4 cut(s) | 187, 427, 445, 586 |
| SmoI | CTYRAG | 4 cut(s) | 187, 427, 445, 586 |
| SsiI | CCGC | 1 cut(s) | 1326 |
| SspMI | CTAG | 7 cut(s) | 317, 713, 776, 941, 1020, 1115, 1239 |
| StyD4I | CCNGG | 3 cut(s) | 238, 989, 1166 |
| StyI | CCWWGG | 4 cut(s) | 517, 790, 1299, 1614 |
| TaaI | ACNGT | 1 cut(s) | 1677 |
| TaiI | ACGT | 1 cut(s) | 1361 |
| TaqI | TCGA | 5 cut(s) | 19, 202, 691, 1310, 1427 |
| TatI | WGTACW | 1 cut(s) | 637 |
| TfiI | GAWTC | 5 cut(s) | 235, 533, 598, 752, 1375 |
| Tru1I | TTAA | 8 cut(s) | 12, 227, 587, 611, 642, 647, 830, 1139 |
| Tru9I | TTAA | 8 cut(s) | 12, 227, 587, 611, 642, 647, 830, 1139 |
| TscAI | CASTG | 4 cut(s) | 64, 611, 814, 964 |
| TseFI | GTSAC | 1 cut(s) | 1026 |
| TseI | GCWGC | 1 cut(s) | 912 |
| Tsp45I | GTSAC | 1 cut(s) | 1026 |
| TspDTI | ATGAA | 7 cut(s) | 27, 114, 285, 353, 1404, 1473, 1614 |
| TspGWI | ACGGA | 1 cut(s) | 987 |
| TspRI | CASTG | 4 cut(s) | 64, 611, 814, 964 |
| Vha464I | CTTAAG | 1 cut(s) | 586 |
| VpaK11BI | GGWCC | 4 cut(s) | 217, 323, 1054, 1296 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1482 |
| XapI | RAATTY | 5 cut(s) | 743, 875, 1046, 1175, 1332 |
| XbaI | TCTAGA | 1 cut(s) | 1114 |
| XceI | RCATGY | 2 cut(s) | 854, 1292 |
| XmiI | GTMKAC | 1 cut(s) | 350 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1175, 1687 |
| XspI | CTAG | 7 cut(s) | 317, 713, 776, 941, 1020, 1115, 1239 |
| ZrmI | AGTACT | 1 cut(s) | 639 |