MD12G1174400.v1.1

Receptor-like protein 12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000047
Physical Location & Seq
Forward (+)
25457213 .. 25467840
10628 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1174400.v1.1.491

Sequence Viewer

Length: 3414 bp
ATGAAAACTCCTCTGCTTTCATGGCTTTTCTTACTCCGCATTTGCTATGTTTCACTCACCTTCCATACGCTTGTGGTCTCTACCCAATGTCCCGGCGATCAGCAATCTTTGTTGCTAAAATTGAAGAACACCCTCGCATTTAACCATACAGCGTCTAAAAAACTTGTGAAGTGGAAGAATGGATCGGATTACTGCTCTTGGGAAGGAGTGTCCTGCAAAAAGGGTTGTGTTTCGAATCTGGACTTGAGCAGCGAGGCGATTAGTGGTGGACTTGATAATTCGAGTTCTCTTTTCAGTCTGAAGTCAATCGAGAACCTGAATTTGGCTTACAATAACTTCAACTACACTCAGATTCCTTCCGAGTTCAAGCAGCTAACCCGATTGATTAATCTGAACTTGTCAAATGCTGGCTTTGCGGGGCAGGTTCCGATTGAGATTTCGCACTTGACGAGGTTGGTAACTCTTGATTTGTCTACCTTTTATTTCCCCGGAACTCCTTCGTTGAATCTTGAGAATCCGAAATTGAATGTGCTGCTTGCGAATTTTTCTGAGCTTGTTGAACTTTATCTTGATGGTGTGAATATATCAGCACAGGGGACCCATTGGTGCCAAGCGATATCATCTTCATTGCCAAAACTGAGGGTGTTGAGCTTGTCCACTTGTAATCTTTCAGGCCCTATTGATAGTTCACTGCTGAAGCTTCAGTCTCTCTCAGTTATTCGTATTGAGAACAACAATTTGTCTACTAAAGTTCCAGAATTCTTCTCGAATTTCCCGAATTTGACTTCCTTACGAATGGTGAATTCTGGATTACATGGTGCATTCCCAAAGAAGATCTTCCAGGTACCTACACTGCAGACTATTGACTTATCTGATAATGCGCAGCTTCAAGGTTCCTTACCAGAATTTCCGAAGAATGGATCTCTTCGATCCCTGGTTCTCAATGGGGCAAATTTTTCAGGCCAGTTGCTTCCAAACTCTATTGGGAACCTCGAATTCCTGTCCAAAATAGATATTGCAACTTGCAATTTCACTGGATCAATCCCGAAGTCAATCGAAGACCTTACGCAGTTGGTTTATCTGGACTTGGCGACGAACAAGTTCAATGGTTCAGTTCCATCCTTCAGTATGGCCAAGAATTTGACCCTAATAAATCTTTCCTACAATCAGCTAACGGGTCAGATTAATTCCTCGCACTGGGAAAACCTGACTAATCTGGTGAATCTCGACTTGCGACACAATCTACTTGATGGGACTATTCCGTCATCTCTGTTTTCTCTTCCTTTGCTGAAGAAACTACAGCTTTCTAACAATCAATTCTCTGGTCTGCTGCTTGAATTTGCTAATAACTCTGTACTGGACACCCTTGATTTGAGTAGCAACAAGTTGGAAGGTTCAATTCCCAAGTCTATCTTGAATTTCCAAGGGCTTAAGATTCTTTTACTTTCTTCAAACAACTTCACTGGCTCTTTCCTTCTGAATGATATTCAGCAGTTAAGAAATCTTTCGAATCTTGATCTTTCTTTCAATAGCTTGTCTATTAATTACAATGACATCAATTCCTCTCCTTCTTCATTTTCAAATATTACCACATTGAAGTTAGCTTCTTGCAATTTGAGAAGAATCCCAGGTTTCTTGAGAAGTCAATCAACATTAAACACTTTGGACCTATCAGAAAACCAGATTCATGGTGAGATACCCAACTGGATTTGGAAGTTGAGTAGTCTTAGTCAACTAAATCTTTCTTGCAACTCTCTGGTAAATCTACAAGGTCCTTTCCTCAATCTTACTTCTAATATGTCTGTGCTTGACCTTCATTCCAACCAGCTTCAGGGACAAATTCCATTGCTTCCAAGATTGACCATTTATATGGATTACTCAAGAAATAATTTCAGCTCAAGCATTCCGGCCAACATTGGTGATTTCCTTATGTACACTATGTTCTTCTCTCTTGCAAGCAACAAGTTAAATGGAAGCATTCCAGAATCAATGTGCAAGGCACCATATCTTCAGGTTCTTGATTTGTCCAATAATTCTCTGAGTGGCCCGATTCCCCAATGCTTGACTACCATAAGCGGTGGGACACTGGCAATACTTAATCTAAGGAGAAACAGACTTAATGGTTCTGTTCCTGATGGATTTCCTCAGCATTGCAGTTTAAGAACTCTTGACCTCAATGGAAATCAGATAGAAGGTAAATTTCCAAAATCTCTTGGCAATTGCAGAATGCTTGAGGTTTTAAACATTGGAAACAATCAGATAAGAGATACCTATCCTTGCTTGTTGAAGAAGATATCCTCCTTGCGTGTCCTTGTTTTGCGATCCAACCGTTTTTATGGACACATGGTATGCAACAAAATCAGTGGTACCTGGCCAAAGCTTCAAATTGTTGACATAGCTCGCAACAATTTTAATGGTGAAATACGAGGAAGATGCTTGAGAACCTGGAAAGCAATGATGACTGATGAAAGTGATGCCAAGTCAAAAACTAATCACCTTCGATTTCAATTTCTAAAGTTCAGTCAGGTATACTATCAGGATGCTATAACAGTTACAAATAAAGGTCTGGAAATGGAGTTGGTAAAGATTCTAACTGTTTTCACCTACATTGACATCTCATGCAACAACTTTAGTGGATCAATACCTGCGGAGGTGGGAGAACTCAAATCCCTCTATGGCCTCAACTTGTCCATCAATTCTTTCACAGGCCCAATCCCATCATCATTAGGTAACCTACGACAACTTGAGTCGTTAGACCTTTCAAACAACAGCTTGAGTGGGCAAATTCCAGTGGAGTTTGGGGGCCTTACTTTCCTTTCTTTCATGGATGTCTCAAACAATCAACTGGTCGGGAAGATACCAATCAGTACCCAGATTTCAACATTTCCAGCGGAGTCCTTCGCAGGCAATAAAGGATTATGTGGGCCACCTTCACTTGTAAAATGCAGTGATACCAACGTGTCACCAAATGCAGCACCAAAGGGTCGAGACAAAGCATCAAAAGTTGAGTTTGATTGGCAGTCCATATATACTGGAGTGGGTTATGGAGTGGGAGGAGGAGTCGTTGTTATCCTCTTAATGGTGTGGGAAGAAGGAAGAAACTGGTTAGAGGACAGCATTGACAAAATTCTTCTGGTAATTCTTCCAATGATGGGATATTCTTACAGAACTCATGACGAATGGGACGATGATGAGGAGGACGAAGATTCCGAAGAAGAGAGCACATATATCATGCAAGACTATAGCGGAGACGAGATTGATTCAGAAGACAGAGTGTTTCGGGGTCCATATTGTGTGTTTTGTTCAAAACTTGATATAAGTAGGAAGAGGGCCATCCATAACCCAAACTGTACATGCAGTTTTTCACCACCTATTACATCATTTTCTTCCTCTTCCTACTCATTTTCTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009888 GO:0010073 GO:0032501 GO:0032502 GO:0048507 GO:0048856
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

1138

Amino Acids

125.81

Weight (kDa)

6.43

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 72 1.5e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 528 - 586 7.2e-07 Leucine rich repeat
LRR_8 PF13855 695 - 754 9.2e-07 Leucine rich repeat
LRR_8 PF13855 719 - 778 3.5e-07 Leucine rich repeat
LRR_8 PF13855 890 - 949 6.3e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000047)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g13590 FvH4_6g10800 FvH4_6g11080 FvH4_6g11081 FvH4_6g11660 FvH4_6g11660 FvH4_6g11660 FvH4_6g11660 FvH4_6g11660 FvH4_6g21370 FvH4_6g21371 FvH4_6g21371 FvH4_6g21371 FvH4_6g21371 FvH4_6g21371 FvH4_6g21371 FvH4_6g21371 FvH4_6g21373 FvH4_6g21374 FvH4_6g21375 FvH4_6g21378 FvH4_7g13570
malus_domestica MD00G1166300.v1.1 MD04G1159400.v1.1 MD04G1159500.v1.1 MD04G1159700.v1.1 MD04G1159800.v1.1 MD04G1160600.v1.1 MD04G1160700.v1.1 MD04G1160800.v1.1 MD04G1161000.v1.1 MD04G1161100.v1.1 MD04G1161200.v1.1 MD04G1161300.v1.1 MD04G1161600.v1.1 MD04G1161700.v1.1 MD04G1210900.v1.1 MD05G1095100.v1.1 MD05G1095300.v1.1 MD05G1095400.v1.1 MD05G1095600.v1.1 MD08G1062000.v1.1 MD08G1062100.v1.1 MD12G1152900.v1.1 MD12G1153300.v1.1 MD12G1172900.v1.1 MD12G1173400.v1.1 MD12G1173600.v1.1 MD12G1173700.v1.1 MD12G1173800.v1.1 MD12G1174000.v1.1 MD12G1174100.v1.1 MD12G1174400.v1.1 MD12G1175000.v1.1
prunus_persica Prupe.6G281000_v2.0.a1 Prupe.6G281100_v2.0.a1 Prupe.6G281200_v2.0.a1 Prupe.6G281200_v2.0.a1 Prupe.6G281200_v2.0.a1 Prupe.6G281200_v2.0.a1 Prupe.6G281300_v2.0.a1 Prupe.6G281400_v2.0.a1 Prupe.6G281400_v2.0.a1 Prupe.6G281400_v2.0.a1 Prupe.6G281500_v2.0.a1 Prupe.6G281500_v2.0.a1 Prupe.6G288800_v2.0.a1 Prupe.7G088700_v2.0.a1 Prupe.7G088700_v2.0.a1 Prupe.8G054300_v2.0.a1 Prupe.8G054400_v2.0.a1
pyrus_communis pycom04g14110 pycom04g14120 pycom04g14130 pycom04g14180 pycom04g14390 pycom04g14410 pycom04g14420 pycom04g14430 pycom04g14450 pycom04g14460 pycom12g14600 pycom12g14610 pycom12g16390 pycom12g16430 pycom12g16460 pycom12g16470 pycom12g16480 pycom12g16490 pycom12g16580 pycom12g16600
rosa_chinensis RchiOBHm_Chr0c32g0501501 RchiOBHm_Chr0c32g0501511 RchiOBHm_Chr0c32g0501521 RchiOBHm_Chr0c39g0503191 RchiOBHm_Chr0c39g0503201 RchiOBHm_Chr0c39g0503231 RchiOBHm_Chr0c39g0503261 RchiOBHm_Chr0c39g0503271 RchiOBHm_Chr0c39g0503311 RchiOBHm_Chr0c39g0503331 RchiOBHm_Chr1g0316151 RchiOBHm_Chr1g0316251 RchiOBHm_Chr1g0316291 RchiOBHm_Chr1g0351861 RchiOBHm_Chr1g0351911 RchiOBHm_Chr1g0353121 RchiOBHm_Chr1g0353151 RchiOBHm_Chr1g0353161 RchiOBHm_Chr1g0353221 RchiOBHm_Chr1g0353251 RchiOBHm_Chr1g0353271 RchiOBHm_Chr1g0353291 RchiOBHm_Chr1g0353341 RchiOBHm_Chr3g0451491 RchiOBHm_Chr3g0461851 RchiOBHm_Chr3g0461931 RchiOBHm_Chr3g0462001 RchiOBHm_Chr3g0462441 RchiOBHm_Chr3g0462461 RchiOBHm_Chr3g0462511 RchiOBHm_Chr3g0463331 RchiOBHm_Chr3g0463341 RchiOBHm_Chr3g0463351 RchiOBHm_Chr3g0477991 RchiOBHm_Chr3g0478021 RchiOBHm_Chr3g0478031 RchiOBHm_Chr3g0478051 RchiOBHm_Chr3g0478091 RchiOBHm_Chr3g0478101 RchiOBHm_Chr3g0478141 RchiOBHm_Chr3g0478161 RchiOBHm_Chr3g0478181 RchiOBHm_Chr3g0478191 RchiOBHm_Chr3g0478311 RchiOBHm_Chr3g0478331 RchiOBHm_Chr3g0478381 RchiOBHm_Chr3g0478431 RchiOBHm_Chr3g0478451 RchiOBHm_Chr3g0478491 RchiOBHm_Chr3g0478511 RchiOBHm_Chr3g0478551 RchiOBHm_Chr3g0478561 RchiOBHm_Chr3g0478581 RchiOBHm_Chr3g0478601 RchiOBHm_Chr3g0481181 RchiOBHm_Chr3g0483751 RchiOBHm_Chr3g0483761 RchiOBHm_Chr3g0483771 RchiOBHm_Chr3g0483781 RchiOBHm_Chr5g0037311 RchiOBHm_Chr5g0039781 RchiOBHm_Chr5g0039851 RchiOBHm_Chr5g0048141 RchiOBHm_Chr6g0257231
rosa_laevigata RLG00000008156 RLG00000019550 RLG00000019551 RLG00000022912 RLG00000022914 RLG00000023271 RLG00000023639 RLG00000023640 RLG00000023644 RLG00000023646 RLG00000023648 RLG00000023650 RLG00000023654 RLG00000023658 RLG00000023660 RLG00000023661 RLG00000023662 RLG00000023664 RLG00000023666 RLG00000023670 RLG00000023671 RLG00000023672 RLG00000023673 RLG00000023882 RLG00000024789 RLG00000024854 RLG00000024855 RLG00000024856 RLG00000024859 RLG00000024860 RLG00000024886 RLG00000024891 RLG00000024892 RLG00000024972 RLG00000028274 RLG00000028275 RLG00000028277 RLG00000028279 RLG00000028281 RLG00000028283 RLG00000028285 RLG00000028287 RLG00000028376 RLG00000030078 RLG00000030682 RLG00000030690 RLG00000033943 RLG00000033946
rosa_multiflora Rmu_co8376673.1_g000001 Rmu_sc0000103.1_g000026 Rmu_sc0000295.1_g000035 Rmu_sc0000923.1_g000003 Rmu_sc0000923.1_g000005 Rmu_sc0001207.1_g000038 Rmu_sc0001207.1_g000054 Rmu_sc0001207.1_g000059 Rmu_sc0001207.1_g000069 Rmu_sc0001207.1_g000073 Rmu_sc0001207.1_g000080 Rmu_sc0001218.1_g000002 Rmu_sc0001218.1_g000019 Rmu_sc0001218.1_g000039 Rmu_sc0001218.1_g000041 Rmu_sc0001218.1_g000042 Rmu_sc0001218.1_g000047 Rmu_sc0001228.1_g000001 Rmu_sc0001333.1_g000006 Rmu_sc0001431.1_g000002 Rmu_sc0001431.1_g000003 Rmu_sc0001487.1_g000001 Rmu_sc0001518.1_g000007 Rmu_sc0001518.1_g000008 Rmu_sc0001933.1_g000003 Rmu_sc0002151.1_g000010 Rmu_sc0002151.1_g000011 Rmu_sc0002531.1_g000014 Rmu_sc0002531.1_g000017 Rmu_sc0003078.1_g000003 Rmu_sc0003078.1_g000006 Rmu_sc0003078.1_g000013 Rmu_sc0003078.1_g000018 Rmu_sc0003078.1_g000020 Rmu_sc0003326.1_g000001 Rmu_sc0003624.1_g000002 Rmu_sc0003624.1_g000010 Rmu_sc0003624.1_g000011 Rmu_sc0003624.1_g000013 Rmu_sc0003624.1_g000014 Rmu_sc0003850.1_g000006 Rmu_sc0003850.1_g000007 Rmu_sc0004308.1_g000017 Rmu_sc0004308.1_g000020 Rmu_sc0004308.1_g000025 Rmu_sc0004778.1_g000001 Rmu_sc0004888.1_g000006 Rmu_sc0005196.1_g000010 Rmu_sc0005196.1_g000011 Rmu_sc0005313.1_g000001 Rmu_sc0005657.1_g000033 Rmu_sc0005657.1_g000044 Rmu_sc0005657.1_g000052 Rmu_sc0005657.1_g000054 Rmu_sc0005657.1_g000059 Rmu_sc0005657.1_g000064 Rmu_sc0005657.1_g000071 Rmu_sc0005767.1_g000001 Rmu_sc0005767.1_g000005 Rmu_sc0005767.1_g000007 Rmu_sc0005767.1_g000023 Rmu_sc0006119.1_g000004 Rmu_sc0006539.1_g000002 Rmu_sc0006651.1_g000017 Rmu_sc0006651.1_g000021 Rmu_sc0006651.1_g000022 Rmu_sc0007105.1_g000001 Rmu_sc0007641.1_g000001 Rmu_sc0007641.1_g000006 Rmu_sc0007728.1_g000003 Rmu_sc0007777.1_g000005 Rmu_sc0007777.1_g000008 Rmu_sc0007777.1_g000010 Rmu_sc0007777.1_g000019 Rmu_sc0007777.1_g000020 Rmu_sc0007777.1_g000022 Rmu_sc0008105.1_g000002 Rmu_sc0008340.1_g000002 Rmu_sc0008559.1_g000005 Rmu_sc0008560.1_g000002 Rmu_sc0008866.1_g000005 Rmu_sc0011028.1_g000006 Rmu_sc0011028.1_g000009 Rmu_sc0011346.1_g000003 Rmu_sc0011346.1_g000004 Rmu_sc0011346.1_g000012 Rmu_sc0011461.1_g000002 Rmu_sc0013048.1_g000001 Rmu_sc0015960.1_g000001 Rmu_sc0026214.1_g000001
rosa_roxburghii Rroxscaffold_1G00041310 Rroxscaffold_1G00041400 Rroxscaffold_1G00041410 Rroxscaffold_4G00301900 Rroxscaffold_4G00301910 Rroxscaffold_4G00301970 Rroxscaffold_4G00301990 Rroxscaffold_4G00302010 Rroxscaffold_4G00302030 Rroxscaffold_4G00303460 Rroxscaffold_6G00403270 Rroxscaffold_6G00403300 Rroxscaffold_6G00403320 Rroxscaffold_6G00403330 Rroxscaffold_6G00403370 Rroxscaffold_6G00403380 Rroxscaffold_6G00403410 Rroxscaffold_6G00403420 Rroxscaffold_6G00403430 Rroxscaffold_6G00403480 Rroxscaffold_6G00403540 Rroxscaffold_6G00406750 Rroxscaffold_6G00416600 Rroxscaffold_6G00416620 Rroxscaffold_6G00416630 Rroxscaffold_6G00416640 Rroxscaffold_6G00417470 Rroxscaffold_6G00417510 Rroxscaffold_6G00417810 Rroxscaffold_6G00417860 Rroxscaffold_6G00419140
rosa_rugosa Rorug01G0009600 Rorug01G0222400 Rorug01G0222700 Rorug01G0222900 Rorug01G0233900 Rorug01G0234100 Rorug01G0234200 Rorug01G0234400 Rorug01G0235200 Rorug03G0054200 Rorug03G0056600 Rorug03G0056700 Rorug03G0056800 Rorug03G0056900 Rorug03G0057200 Rorug03G0064100 Rorug03G0064100 Rorug03G0064200 Rorug03G0165200 Rorug03G0165300 Rorug03G0165300 Rorug03G0165500 Rorug03G0165500 Rorug03G0165600 Rorug03G0165700 Rorug03G0165900 Rorug03G0166000 Rorug03G0166200 Rorug03G0166300 Rorug03G0166400 Rorug03G0166500 Rorug03G0166700 Rorug04G0127000 Rorug05G0182500 Rorug05G0182900 Rorug05G0183000
rosa_samantha Rh1DG234000 Rh1DG243600 Rh1DG243700 Rh2AG645000 Rh3AG115000 Rh3BG118400 Rh3CG121200 Rh3CG121300 Rh3CG121700 Rh3CG121800 Rh3CG121900 Rh3CG128300 Rh3CG128500 Rh3CG243700 Rh3CG244200 Rh3CG244500 Rh3CG246000 Rh3CG246300 Rh3CG246400 Rh3CG246500 Rh3CG247000 Rh3DG120400 Rh4DG032600 Rh5AG319500 Rh5DG280900 Rh5DG337500 Rh6CG079500
rosa_wichuraiana Rw0G003790 Rw0G006960 Rw0G010120 Rw0G018070 Rw1G001290 Rw1G020570 Rw1G020590 Rw1G021380 Rw1G021390 Rw1G021440 Rw1G021540 Rw2G052980 Rw3G009520 Rw3G009740 Rw3G010350 Rw3G019410 Rw3G019560 Rw3G019590 Rw3G019600 Rw3G019610 Rw3G019620 Rw3G019630 Rw3G019650 Rw4G015840 Rw5G025130 Rw5G025840 Rw6G007960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1261
Acc16I TGCGCA 1 cut(s) 882
Acc36I ACCTGC 2 cut(s) 412, 2653
Acc65I GGTACC 2 cut(s) 844, 2366
AccB1I GGYRCC 4 cut(s) 606, 844, 1999, 2366
AccI GTMKAC 3 cut(s) 473, 743, 2529
AciI CCGC 6 cut(s) 37, 416, 2076, 2648, 2891, 3246
AclWI GGATC 6 cut(s) 190, 924, 928, 1045, 2316, 2644
AcoI YGGCCR 3 cut(s) 1131, 1908, 2372
AcuI CTGAAG 7 cut(s) 320, 686, 716, 1108, 1310, 1814, 1994
AfaI GTAC 6 cut(s) 846, 1356, 1934, 2368, 2869, 3352
AfiI CCNNNNNNNGG 6 cut(s) 322, 917, 1197, 1831, 3079, 3152
AflII CTTAAG 1 cut(s) 1430
AflIII ACRYGT 1 cut(s) 2958
AjnI CCWGG 5 cut(s) 840, 933, 1627, 2369, 2444
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
Alw21I GWGCWC 1 cut(s) 3224
Alw26I GTCTC 5 cut(s) 82, 711, 2836, 2982, 3243
AlwI GGATC 6 cut(s) 190, 924, 928, 1045, 2316, 2644
ApeKI GCWGC 6 cut(s) 249, 370, 532, 883, 1330, 2972
AseI ATTAAT 3 cut(s) 387, 1185, 1542
Asp700I GAANNNNTTC 4 cut(s) 496, 836, 1100, 1504
Asp718I GGTACC 2 cut(s) 844, 2366
AspLEI GCGC 1 cut(s) 883
AsuC2I CCSGG 2 cut(s) 93, 489
AsuII TTCGAA 2 cut(s) 233, 1508
AvaII GGWCC 4 cut(s) 597, 1666, 1772, 3284
BaeI ACNNNNGTAYC 2 cut(s) 2943, 2976
BalI TGGCCA 2 cut(s) 1133, 2374
BanI GGYRCC 4 cut(s) 606, 844, 1999, 2366
BbsI GAAGAC 2 cut(s) 1065, 3273
Bbv12I GWGCWC 1 cut(s) 3224
BbvCI CCTCAGC 1 cut(s) 2145
BbvI GCAGC 6 cut(s) 261, 382, 519, 895, 1317, 2984
BccI CCATC 8 cut(s) 566, 1126, 1244, 2129, 2699, 2725, 3145, 3341
BcgI CGANNNNNNTGC 2 cut(s) 2415, 2449
BciT130I CCWGG 5 cut(s) 842, 935, 1629, 2371, 2446
BcnI CCSGG 2 cut(s) 93, 489
BcoDI GTCTC 5 cut(s) 82, 711, 2836, 2982, 3243
BfmI CTRYAG 3 cut(s) 854, 1298, 3241
BfrI CTTAAG 1 cut(s) 1430
BfuAI ACCTGC 2 cut(s) 412, 2653
BglII AGATCT 1 cut(s) 834
BisI GCNGC 6 cut(s) 250, 371, 533, 884, 1331, 2973
BlsI GCNGC 6 cut(s) 251, 372, 534, 885, 1332, 2974
Bme1390I CCNGG 7 cut(s) 93, 489, 842, 935, 1629, 2371, 2446
Bme18I GGWCC 4 cut(s) 597, 1666, 1772, 3284
BmrFI CCNGG 7 cut(s) 93, 489, 842, 935, 1629, 2371, 2446
BmrI ACTGGG 1 cut(s) 1207
BmsI GCATC 4 cut(s) 2423, 2464, 2530, 3005
BmuI ACTGGG 1 cut(s) 1207
BpiI GAAGAC 2 cut(s) 1065, 3273
BpmI CTGGAG 1 cut(s) 3054
Bpu10I CCTNAGC 1 cut(s) 2145
Bpu14I TTCGAA 2 cut(s) 233, 1508
BpuEI CTTGAG 9 cut(s) 265, 530, 1657, 1864, 1882, 2252, 2458, 2765, 2794
BpuMI CCSGG 2 cut(s) 93, 489
BsaBI GATNNNNATC 2 cut(s) 2271, 2861
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 4 cut(s) 487, 933, 1423, 1627
BsaXI ACNNNNNCTCC 6 cut(s) 3045, 3048, 3051, 3075, 3078, 3081
Bsc4I CCNNNNNNNGG 6 cut(s) 322, 917, 1197, 1831, 3079, 3152
Bse3DI GCAATG 4 cut(s) 626, 1844, 2149, 2460
Bse8I GATNNNNATC 2 cut(s) 2271, 2861
BseBI CCWGG 5 cut(s) 842, 935, 1629, 2371, 2446
BseDI CCNNGG 4 cut(s) 487, 933, 1423, 1627
BseGI GGATG 4 cut(s) 1118, 2545, 2833, 3333
BseJI GATNNNNATC 2 cut(s) 2271, 2861
BseLI CCNNNNNNNGG 6 cut(s) 322, 917, 1197, 1831, 3079, 3152
BseMI GCAATG 4 cut(s) 626, 1844, 2149, 2460
BseMII CTCAG 6 cut(s) 362, 540, 629, 726, 2030, 2159
BseRI GAGGAG 3 cut(s) 3069, 3072, 3209
BseXI GCAGC 6 cut(s) 261, 382, 519, 895, 1317, 2984
BshNI GGYRCC 4 cut(s) 606, 844, 1999, 2366
BsiHKAI GWGCWC 1 cut(s) 3224
BsiSI CCGG 3 cut(s) 93, 489, 1907
BslFI GGGAC 6 cut(s) 75, 610, 1267, 1848, 2095, 3197
BslI CCNNNNNNNGG 6 cut(s) 322, 917, 1197, 1831, 3079, 3152
BsmAI GTCTC 5 cut(s) 82, 711, 2836, 2982, 3243
BsmBI CGTCTC 1 cut(s) 3243
BsmFI GGGAC 6 cut(s) 75, 610, 1267, 1848, 2095, 3197
BsmI GAATGC 4 cut(s) 821, 1902, 1977, 2232
Bso31I GGTCTC 1 cut(s) 82
Bsp119I TTCGAA 2 cut(s) 233, 1508
Bsp1286I GDGCHC 1 cut(s) 3224
Bsp1407I TGTACA 2 cut(s) 1932, 3350
Bsp143I GATC 9 cut(s) 97, 182, 834, 920, 929, 1037, 1516, 2321, 2636
BspACI CCGC 6 cut(s) 37, 416, 2076, 2648, 2891, 3246
BspCNI CTCAG 6 cut(s) 361, 541, 630, 725, 2031, 2158
BspHI TCATGA 1 cut(s) 3172
BspMAI CTGCAG 1 cut(s) 858
BspMI ACCTGC 2 cut(s) 412, 2653
BspPI GGATC 6 cut(s) 190, 924, 928, 1045, 2316, 2644
BspT104I TTCGAA 2 cut(s) 233, 1508
BspT107I GGYRCC 4 cut(s) 606, 844, 1999, 2366
BspTI CTTAAG 1 cut(s) 1430
BspTNI GGTCTC 1 cut(s) 82
BsrDI GCAATG 4 cut(s) 626, 1844, 2149, 2460
BsrGI TGTACA 2 cut(s) 1932, 3350
BssECI CCNNGG 4 cut(s) 487, 933, 1423, 1627
BssMI GATC 9 cut(s) 97, 182, 834, 920, 929, 1037, 1516, 2321, 2636
BssNAI GTATAC 1 cut(s) 2530
BssT1I CCWWGG 1 cut(s) 1423
Bst1107I GTATAC 1 cut(s) 2530
Bst2UI CCWGG 5 cut(s) 842, 935, 1629, 2371, 2446
Bst4CI ACNGT 4 cut(s) 2330, 2551, 2596, 3350
Bst6I CTCTTC 5 cut(s) 930, 1284, 3210, 3320, 3397
BstAFI CTTAAG 1 cut(s) 1430
BstAUI TGTACA 2 cut(s) 1932, 3350
BstBI TTCGAA 2 cut(s) 233, 1508
BstC8I GCNNGC 5 cut(s) 409, 537, 1957, 2401, 2905
BstDEI CTNAG 8 cut(s) 348, 549, 638, 712, 1727, 2039, 2102, 2145
BstEII GGTNACC 1 cut(s) 2729
BstF5I GGATG 4 cut(s) 1118, 2545, 2833, 3333
BstHHI GCGC 1 cut(s) 883
BstKTI GATC 9 cut(s) 100, 185, 837, 923, 932, 1040, 1519, 2324, 2639
BstMAI GTCTC 5 cut(s) 82, 711, 2836, 2982, 3243
BstMBI GATC 9 cut(s) 97, 182, 834, 920, 929, 1037, 1516, 2321, 2636
BstMWI GCNNNNNNNGC 2 cut(s) 22, 413
BstNI CCWGG 5 cut(s) 842, 935, 1629, 2371, 2446
BstNSI RCATGY 1 cut(s) 3357
BstPI GGTNACC 1 cut(s) 2729
BstSCI CCNGG 7 cut(s) 91, 487, 840, 933, 1627, 2369, 2444
BstSFI CTRYAG 3 cut(s) 854, 1298, 3241
BstV1I GCAGC 6 cut(s) 261, 382, 519, 895, 1317, 2984
BstV2I GAAGAC 2 cut(s) 1065, 3273
BstX2I RGATCY 2 cut(s) 834, 920
BstXI CCANNNNNNTGG 2 cut(s) 1688, 1870
BstYI RGATCY 2 cut(s) 834, 920
BstZ17I GTATAC 1 cut(s) 2530
BtsCI GGATG 4 cut(s) 1118, 2545, 2833, 3333
BtsI GCAGTG 3 cut(s) 689, 851, 2953
BtsIMutI CAGTG 9 cut(s) 689, 851, 1032, 1195, 1461, 2084, 2368, 2796, 2953
BveI ACCTGC 2 cut(s) 412, 2653
Cac8I GCNNGC 5 cut(s) 409, 537, 1957, 2401, 2905
CciI TCATGA 1 cut(s) 3172
CfoI GCGC 1 cut(s) 883
CseI GACGC 1 cut(s) 141
Csp6I GTAC 6 cut(s) 845, 1355, 1933, 2367, 2868, 3351
CspCI CAANNNNNGTGG 4 cut(s) 2344, 2379, 2614, 2649
CviAII CATG 9 cut(s) 21, 815, 1688, 2344, 2619, 2824, 3173, 3232, 3354
CviQI GTAC 6 cut(s) 845, 1355, 1933, 2367, 2868, 3351
DdeI CTNAG 8 cut(s) 348, 549, 638, 712, 1727, 2039, 2102, 2145
DpnI GATC 9 cut(s) 99, 184, 836, 922, 931, 1039, 1518, 2323, 2638
DpnII GATC 9 cut(s) 97, 182, 834, 920, 929, 1037, 1516, 2321, 2636
DraI TTTAAA 1 cut(s) 2241
DrdI GACNNNNNNGTC 1 cut(s) 1261
DseDI GACNNNNNNGTC 1 cut(s) 1261
EaeI YGGCCR 3 cut(s) 1131, 1908, 2372
Eam1104I CTCTTC 5 cut(s) 930, 1284, 3210, 3320, 3397
EarI CTCTTC 5 cut(s) 930, 1284, 3210, 3320, 3397
Eco130I CCWWGG 1 cut(s) 1423
Eco31I GGTCTC 1 cut(s) 82
Eco32I GATATC 2 cut(s) 618, 2295
Eco47I GGWCC 4 cut(s) 597, 1666, 1772, 3284
Eco57I CTGAAG 7 cut(s) 320, 686, 716, 1108, 1310, 1814, 1994
Eco91I GGTNACC 1 cut(s) 2729
EcoO109I RGGNCCY 4 cut(s) 597, 674, 1772, 2803
EcoO65I GGTNACC 1 cut(s) 2729
EcoRI GAATTC 3 cut(s) 758, 802, 995
EcoRII CCWGG 5 cut(s) 840, 933, 1627, 2369, 2444
EcoRV GATATC 2 cut(s) 618, 2295
EcoT14I CCWWGG 1 cut(s) 1423
ErhI CCWWGG 1 cut(s) 1423
Esp3I CGTCTC 1 cut(s) 3243
FaeI CATG 9 cut(s) 24, 818, 1691, 2347, 2622, 2827, 3176, 3235, 3357
FalI AAGNNNNNCTT 4 cut(s) 821, 853, 1397, 1429
FaqI GGGAC 6 cut(s) 75, 610, 1267, 1848, 2095, 3197
FatI CATG 9 cut(s) 20, 814, 1687, 2343, 2618, 2823, 3172, 3231, 3353
FauI CCCGC 1 cut(s) 409
FblI GTMKAC 3 cut(s) 473, 743, 2529
Fnu4HI GCNGC 6 cut(s) 250, 371, 533, 884, 1331, 2973
FokI GGATG 4 cut(s) 1105, 2552, 2840, 3320
Fsp4HI GCNGC 6 cut(s) 250, 371, 533, 884, 1331, 2973
FspI TGCGCA 1 cut(s) 882
GlaI GCGC 1 cut(s) 882
GluI GCNGC 6 cut(s) 250, 371, 533, 884, 1331, 2973
GsuI CTGGAG 1 cut(s) 3054
HapII CCGG 3 cut(s) 93, 489, 1907
HgaI GACGC 1 cut(s) 141
HhaI GCGC 1 cut(s) 883
Hin1II CATG 9 cut(s) 24, 818, 1691, 2347, 2622, 2827, 3176, 3235, 3357
Hin6I GCGC 1 cut(s) 881
HinP1I GCGC 1 cut(s) 881
HincII GTYRAC 2 cut(s) 1733, 2392
HindII GTYRAC 2 cut(s) 1733, 2392
HindIII AAGCTT 2 cut(s) 698, 2378
HpaII CCGG 3 cut(s) 93, 489, 1907
Hpy166II GTNNAC 9 cut(s) 269, 474, 657, 689, 744, 1733, 1935, 2392, 2530
Hpy8I GTNNAC 9 cut(s) 269, 474, 657, 689, 744, 1733, 1935, 2392, 2530
Hpy99I CGWCG 1 cut(s) 1096
HpyCH4III ACNGT 4 cut(s) 2330, 2551, 2596, 3350
HpyCH4IV ACGT 1 cut(s) 2958
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 413
HpyF3I CTNAG 8 cut(s) 348, 549, 638, 712, 1727, 2039, 2102, 2145
HpySE526I ACGT 1 cut(s) 2958
Hsp92II CATG 9 cut(s) 24, 818, 1691, 2347, 2622, 2827, 3176, 3235, 3357
HspAI GCGC 1 cut(s) 881
KflI GGGWCCC 1 cut(s) 597
KpnI GGTACC 2 cut(s) 848, 2370
Kzo9I GATC 9 cut(s) 97, 182, 834, 920, 929, 1037, 1516, 2321, 2636
Lsp1109I GCAGC 6 cut(s) 261, 382, 519, 895, 1317, 2984
LweI GCATC 4 cut(s) 2423, 2464, 2530, 3005
MaeII ACGT 1 cut(s) 2958
MaeIII GTNAC 4 cut(s) 457, 2551, 2729, 2961
MalI GATC 9 cut(s) 99, 184, 836, 922, 931, 1039, 1518, 2323, 2638
MboI GATC 9 cut(s) 97, 182, 834, 920, 929, 1037, 1516, 2321, 2636
MfeI CAATTG 1 cut(s) 2218
MflI RGATCY 2 cut(s) 834, 920
MhlI GDGCHC 1 cut(s) 3224
MlsI TGGCCA 2 cut(s) 1133, 2374
MluNI TGGCCA 2 cut(s) 1133, 2374
MlyI GAGTC 3 cut(s) 2756, 2903, 3069
MmeI TCCRAC 3 cut(s) 1368, 1845, 2349
Mox20I TGGCCA 2 cut(s) 1133, 2374
MroXI GAANNNNTTC 4 cut(s) 496, 836, 1100, 1504
MscI TGGCCA 2 cut(s) 1133, 2374
MslI CAYNNNNRTG 1 cut(s) 1868
Msp20I TGGCCA 2 cut(s) 1133, 2374
MspA1I CMGCKG 1 cut(s) 2891
MspCI CTTAAG 1 cut(s) 1430
MspI CCGG 3 cut(s) 93, 489, 1907
MspR9I CCNGG 7 cut(s) 93, 489, 842, 935, 1629, 2371, 2446
MunI CAATTG 1 cut(s) 2218
Mva1269I GAATGC 4 cut(s) 821, 1902, 1977, 2232
MvaI CCWGG 5 cut(s) 842, 935, 1629, 2371, 2446
MwoI GCNNNNNNNGC 2 cut(s) 22, 413
NciI CCSGG 2 cut(s) 93, 489
NdeII GATC 9 cut(s) 97, 182, 834, 920, 929, 1037, 1516, 2321, 2636
NlaIII CATG 9 cut(s) 24, 818, 1691, 2347, 2622, 2827, 3176, 3235, 3357
NmuCI GTSAC 1 cut(s) 2961
NsbI TGCGCA 1 cut(s) 882
NspI RCATGY 1 cut(s) 3357
NspV TTCGAA 2 cut(s) 233, 1508
PagI TCATGA 1 cut(s) 3172
PcsI WCGNNNNNNNCGW 4 cut(s) 446, 773, 3183, 3207
PctI GAATGC 4 cut(s) 821, 1902, 1977, 2232
PdmI GAANNNNTTC 4 cut(s) 496, 836, 1100, 1504
PkrI GCNGC 6 cut(s) 251, 372, 534, 885, 1332, 2974
PleI GAGTC 3 cut(s) 2755, 2902, 3068
PpsI GAGTC 3 cut(s) 2755, 2902, 3068
PpuMI RGGWCCY 2 cut(s) 597, 1772
PshBI ATTAAT 3 cut(s) 387, 1185, 1542
Psp5II RGGWCCY 2 cut(s) 597, 1772
Psp6I CCWGG 5 cut(s) 840, 933, 1627, 2369, 2444
PspEI GGTNACC 1 cut(s) 2729
PspGI CCWGG 5 cut(s) 840, 933, 1627, 2369, 2444
PspPPI RGGWCCY 2 cut(s) 597, 1772
PsrI GAACNNNNNNTAC 2 cut(s) 1925, 1957
PstI CTGCAG 1 cut(s) 858
PsuI RGATCY 2 cut(s) 834, 920
RsaI GTAC 6 cut(s) 846, 1356, 1934, 2368, 2869, 3352
RsaNI GTAC 6 cut(s) 845, 1355, 1933, 2367, 2868, 3351
RseI CAYNNNNRTG 1 cut(s) 1868
SatI GCNGC 6 cut(s) 250, 371, 533, 884, 1331, 2973
Sau3AI GATC 9 cut(s) 97, 182, 834, 920, 929, 1037, 1516, 2321, 2636
SchI GAGTC 3 cut(s) 2756, 2903, 3069
ScrFI CCNGG 7 cut(s) 93, 489, 842, 935, 1629, 2371, 2446
SduI GDGCHC 1 cut(s) 3224
SfaNI GCATC 4 cut(s) 2423, 2464, 2530, 3005
SfcI CTRYAG 3 cut(s) 854, 1298, 3241
SfuI TTCGAA 2 cut(s) 233, 1508
SinI GGWCC 4 cut(s) 597, 1666, 1772, 3284
SmiMI CAYNNNNRTG 1 cut(s) 1868
SsiI CCGC 6 cut(s) 37, 416, 2076, 2648, 2891, 3246
SspI AATATT 1 cut(s) 1585
StyD4I CCNGG 7 cut(s) 91, 487, 840, 933, 1627, 2369, 2444
StyI CCWWGG 1 cut(s) 1423
TaaI ACNGT 4 cut(s) 2330, 2551, 2596, 3350
TaiI ACGT 1 cut(s) 2961
TatI WGTACW 3 cut(s) 1354, 1932, 3350
TscAI CASTG 9 cut(s) 696, 858, 1039, 1202, 1468, 2091, 2368, 2796, 2953
TseFI GTSAC 1 cut(s) 2961
TseI GCWGC 6 cut(s) 249, 370, 532, 883, 1330, 2972
Tsp45I GTSAC 1 cut(s) 2961
TspDTI ATGAA 8 cut(s) 9, 17, 615, 1563, 1676, 1805, 2481, 2812
TspGWI ACGGA 1 cut(s) 1251
TspRI CASTG 9 cut(s) 696, 858, 1039, 1202, 1468, 2091, 2368, 2796, 2953
Vha464I CTTAAG 1 cut(s) 1430
VpaK11BI GGWCC 4 cut(s) 597, 1666, 1772, 3284
VspI ATTAAT 3 cut(s) 387, 1185, 1542
XceI RCATGY 1 cut(s) 3357
XmiI GTMKAC 3 cut(s) 473, 743, 2529
XmnI GAANNNNTTC 4 cut(s) 496, 836, 1100, 1504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.