spliceosomal complex assembly
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 224 |
| AclWI | GGATC | 3 cut(s) | 38, 51, 113 |
| AfaI | GTAC | 2 cut(s) | 55, 190 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 101, 250, 277 |
| AgsI | TTSAA | 1 cut(s) | 265 |
| AjnI | CCWGG | 1 cut(s) | 49 |
| AluBI | AGCT | 4 cut(s) | 61, 149, 153, 199 |
| AluI | AGCT | 4 cut(s) | 61, 149, 153, 199 |
| AlwI | GGATC | 3 cut(s) | 38, 51, 113 |
| AoxI | GGCC | 1 cut(s) | 173 |
| ApeKI | GCWGC | 1 cut(s) | 196 |
| AspS9I | GGNCC | 2 cut(s) | 173, 280 |
| AsuNHI | GCTAGC | 1 cut(s) | 149 |
| AvaII | GGWCC | 1 cut(s) | 280 |
| BamHI | GGATCC | 1 cut(s) | 43 |
| BbvI | GCAGC | 1 cut(s) | 208 |
| BceAI | ACGGC | 1 cut(s) | 79 |
| BciT130I | CCWGG | 1 cut(s) | 51 |
| BfaI | CTAG | 1 cut(s) | 150 |
| BglII | AGATCT | 1 cut(s) | 159 |
| BisI | GCNGC | 2 cut(s) | 197, 225 |
| BlsI | GCNGC | 2 cut(s) | 198, 226 |
| Bme1390I | CCNGG | 1 cut(s) | 51 |
| Bme18I | GGWCC | 1 cut(s) | 280 |
| BmgT120I | GGNCC | 2 cut(s) | 173, 280 |
| BmiI | GGNNCC | 1 cut(s) | 45 |
| BmrFI | CCNGG | 1 cut(s) | 51 |
| BmsI | GCATC | 1 cut(s) | 219 |
| BmtI | GCTAGC | 1 cut(s) | 153 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 101, 250, 277 |
| Bse3DI | GCAATG | 1 cut(s) | 234 |
| BseBI | CCWGG | 1 cut(s) | 51 |
| BseGI | GGATG | 1 cut(s) | 349 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 101, 250, 277 |
| BseMI | GCAATG | 1 cut(s) | 234 |
| BseMII | CTCAG | 3 cut(s) | 147, 158, 183 |
| BseRI | GAGGAG | 1 cut(s) | 47 |
| BseXI | GCAGC | 1 cut(s) | 208 |
| BshFI | GGCC | 1 cut(s) | 175 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 101, 250, 277 |
| BsmI | GAATGC | 1 cut(s) | 130 |
| BsnI | GGCC | 1 cut(s) | 175 |
| Bsp143I | GATC | 3 cut(s) | 43, 118, 159 |
| BspACI | CCGC | 1 cut(s) | 224 |
| BspANI | GGCC | 1 cut(s) | 175 |
| BspCNI | CTCAG | 3 cut(s) | 148, 159, 184 |
| BspLI | GGNNCC | 1 cut(s) | 45 |
| BspOI | GCTAGC | 1 cut(s) | 153 |
| BspPI | GGATC | 3 cut(s) | 38, 51, 113 |
| BsrDI | GCAATG | 1 cut(s) | 234 |
| BssMI | GATC | 3 cut(s) | 43, 118, 159 |
| Bst2UI | CCWGG | 1 cut(s) | 51 |
| BstC8I | GCNNGC | 3 cut(s) | 113, 147, 151 |
| BstDEI | CTNAG | 4 cut(s) | 156, 167, 182, 192 |
| BstF5I | GGATG | 1 cut(s) | 349 |
| BstKTI | GATC | 3 cut(s) | 46, 121, 162 |
| BstMBI | GATC | 3 cut(s) | 43, 118, 159 |
| BstNI | CCWGG | 1 cut(s) | 51 |
| BstNSI | RCATGY | 1 cut(s) | 115 |
| BstSCI | CCNGG | 1 cut(s) | 49 |
| BstV1I | GCAGC | 1 cut(s) | 208 |
| BstX2I | RGATCY | 3 cut(s) | 43, 118, 159 |
| BstYI | RGATCY | 3 cut(s) | 43, 118, 159 |
| BsuRI | GGCC | 1 cut(s) | 175 |
| BtgZI | GCGATG | 1 cut(s) | 191 |
| BtsCI | GGATG | 1 cut(s) | 349 |
| Cac8I | GCNNGC | 3 cut(s) | 113, 147, 151 |
| Cfr13I | GGNCC | 2 cut(s) | 173, 280 |
| CsiI | ACCWGGT | 1 cut(s) | 49 |
| Csp6I | GTAC | 2 cut(s) | 54, 189 |
| CviAII | CATG | 2 cut(s) | 112, 127 |
| CviJI | RGCY | 6 cut(s) | 61, 149, 153, 175, 199, 243 |
| CviKI_1 | RGCY | 6 cut(s) | 61, 149, 153, 175, 199, 243 |
| CviQI | GTAC | 2 cut(s) | 54, 189 |
| DdeI | CTNAG | 4 cut(s) | 156, 167, 182, 192 |
| DpnI | GATC | 3 cut(s) | 45, 120, 161 |
| DpnII | GATC | 3 cut(s) | 43, 118, 159 |
| Eco32I | GATATC | 1 cut(s) | 235 |
| Eco47I | GGWCC | 1 cut(s) | 280 |
| EcoO109I | RGGNCCY | 1 cut(s) | 280 |
| EcoRII | CCWGG | 1 cut(s) | 49 |
| EcoRV | GATATC | 1 cut(s) | 235 |
| EcoT22I | ATGCAT | 1 cut(s) | 132 |
| FaeI | CATG | 2 cut(s) | 115, 130 |
| FaiI | YATR | 3 cut(s) | 113, 128, 246 |
| FatI | CATG | 2 cut(s) | 111, 126 |
| Fnu4HI | GCNGC | 2 cut(s) | 197, 225 |
| FokI | GGATG | 1 cut(s) | 336 |
| Fsp4HI | GCNGC | 2 cut(s) | 197, 225 |
| FspBI | CTAG | 1 cut(s) | 150 |
| GluI | GCNGC | 2 cut(s) | 197, 225 |
| HaeIII | GGCC | 1 cut(s) | 175 |
| Hin1II | CATG | 2 cut(s) | 115, 130 |
| HinfI | GANTC | 1 cut(s) | 343 |
| Hpy188I | TCNGA | 3 cut(s) | 157, 168, 277 |
| Hpy188III | TCNNGA | 1 cut(s) | 238 |
| Hpy99I | CGWCG | 1 cut(s) | 277 |
| HpyCH4V | TGCA | 3 cut(s) | 115, 130, 322 |
| HpyF3I | CTNAG | 4 cut(s) | 156, 167, 182, 192 |
| Hsp92II | CATG | 2 cut(s) | 115, 130 |
| Kzo9I | GATC | 3 cut(s) | 43, 118, 159 |
| LpnPI | CCDG | 4 cut(s) | 12, 36, 63, 159 |
| Lsp1109I | GCAGC | 1 cut(s) | 208 |
| LweI | GCATC | 1 cut(s) | 219 |
| MabI | ACCWGGT | 1 cut(s) | 49 |
| MaeI | CTAG | 1 cut(s) | 150 |
| MalI | GATC | 3 cut(s) | 45, 120, 161 |
| MboI | GATC | 3 cut(s) | 43, 118, 159 |
| MboII | GAAGA | 2 cut(s) | 209, 338 |
| MfeI | CAATTG | 1 cut(s) | 323 |
| MflI | RGATCY | 3 cut(s) | 43, 118, 159 |
| MluCI | AATT | 1 cut(s) | 323 |
| MmeI | TCCRAC | 1 cut(s) | 255 |
| MnlI | CCTC | 8 cut(s) | 5, 25, 101, 164, 244, 249, 271, 292 |
| Mph1103I | ATGCAT | 1 cut(s) | 132 |
| MseI | TTAA | 2 cut(s) | 294, 335 |
| MspR9I | CCNGG | 1 cut(s) | 51 |
| MunI | CAATTG | 1 cut(s) | 323 |
| Mva1269I | GAATGC | 1 cut(s) | 130 |
| MvaI | CCWGG | 1 cut(s) | 51 |
| NdeII | GATC | 3 cut(s) | 43, 118, 159 |
| NheI | GCTAGC | 1 cut(s) | 149 |
| NlaIII | CATG | 2 cut(s) | 115, 130 |
| NlaIV | GGNNCC | 1 cut(s) | 45 |
| NsiI | ATGCAT | 1 cut(s) | 132 |
| NspI | RCATGY | 1 cut(s) | 115 |
| PaeI | GCATGC | 1 cut(s) | 115 |
| PctI | GAATGC | 1 cut(s) | 130 |
| PfeI | GAWTC | 1 cut(s) | 343 |
| PkrI | GCNGC | 2 cut(s) | 198, 226 |
| PpuMI | RGGWCCY | 1 cut(s) | 280 |
| Psp5II | RGGWCCY | 1 cut(s) | 280 |
| Psp6I | CCWGG | 1 cut(s) | 49 |
| PspGI | CCWGG | 1 cut(s) | 49 |
| PspN4I | GGNNCC | 1 cut(s) | 45 |
| PspPI | GGNCC | 2 cut(s) | 173, 280 |
| PspPPI | RGGWCCY | 1 cut(s) | 280 |
| PsuI | RGATCY | 3 cut(s) | 43, 118, 159 |
| RsaI | GTAC | 2 cut(s) | 55, 190 |
| RsaNI | GTAC | 2 cut(s) | 54, 189 |
| SaqAI | TTAA | 2 cut(s) | 294, 335 |
| SatI | GCNGC | 2 cut(s) | 197, 225 |
| Sau3AI | GATC | 3 cut(s) | 43, 118, 159 |
| Sau96I | GGNCC | 2 cut(s) | 173, 280 |
| ScrFI | CCNGG | 1 cut(s) | 51 |
| SexAI | ACCWGGT | 1 cut(s) | 49 |
| SfaNI | GCATC | 1 cut(s) | 219 |
| SinI | GGWCC | 1 cut(s) | 280 |
| SphI | GCATGC | 1 cut(s) | 115 |
| Sse9I | AATT | 1 cut(s) | 323 |
| SsiI | CCGC | 1 cut(s) | 224 |
| SspMI | CTAG | 1 cut(s) | 150 |
| StyD4I | CCNGG | 1 cut(s) | 49 |
| TaqI | TCGA | 1 cut(s) | 308 |
| TasI | AATT | 1 cut(s) | 323 |
| TauI | GCSGC | 1 cut(s) | 227 |
| TfiI | GAWTC | 1 cut(s) | 343 |
| Tru1I | TTAA | 2 cut(s) | 294, 335 |
| Tru9I | TTAA | 2 cut(s) | 294, 335 |
| TseI | GCWGC | 1 cut(s) | 196 |
| TspDTI | ATGAA | 2 cut(s) | 194, 338 |
| VpaK11BI | GGWCC | 1 cut(s) | 280 |
| XceI | RCATGY | 1 cut(s) | 115 |
| XspI | CTAG | 1 cut(s) | 150 |
| Zsp2I | ATGCAT | 1 cut(s) | 132 |