Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0000134

Physical Location & Seq

Genomic Coordinates Reverse (-)

13303668 .. 13311028

Gene Structure 7361 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g20240.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGAAGATGGCAATAAGAGATAAGATCATGATGCTCATAATATTGGTTTTATGGTGCTTGACATGCAGAAACTCAATCGCTGTTGAAGCACAGTCTACAAATCTTCCTGCTTCAGAAGTTGAAGCTCTGAAAGAAATAGCTGCACAAATAGGAAAGAAAGATTGGAACTTTAGCGTCGACCCTTGCAGCAATGACACAAGCTGGGCCACCCCAATATCTGCTGCCAGGCCTCTGTTCAACAACACCGTCACCTGTAACTGCTCCTTCTCCGATGGTGCATGCCACGTCACTAGCATCTTTCTTAAAGGGCAAGACCTTGCTGGGGTGCTTCCACCGTCTATTGCAAAGTTACCCTACCTGATCAATGTCGATTTCACGAGGAACTACTTCAATGGCAGCATACCGCAAGAATGGGCGTCGACAAAGTTGCAGTATCTATCCATTACTGTCAACAACTTGTCAGGACCAATTCCGACCTACCTGGGAAACATTTCCACTCTAATTTATCTGAGCGTAGAGAATAACATGTTCTCAGGAATTGTTCCTCCTGAGCTTGGAAACTTGGTTAACTTGCAGAATCTTATTGTTAGTGCAAACAGTCTCACAGGAGAACTGCCGGCCGCTCTTATGAATCTGACTAAGTTAACAGAACTGAGGATTAGCAGCAACAACTTCACTGGAAGAATGCCTGACTCTTTTCAAAGTTGGAATCAACTTCAGAAATTAGAGATCCAAGCTAGTGGTCTACAGGGCCCCATTCCATCTACCATTTCTGTCTTGAGTAATATAACAGAACTGAGGATCAGCGACTTAAACGGAGGGGGTTCAGTATTTCCAAACTTGAGTCATATGACAAACTTGCAAACATTAATGCTCCGGAGCTGTAACTTATCTGGACCTATCCCTTCCGATTTATCAGCCATGTCACAGTTGAAAATATTAGATCTAAGCTTCAACAGATTAGAGGGGAGCATTCCTGATTTTGCAGATATGACACTATTGCAGTTCTTGTATCTAACAAGCAACTTGCTTACCGGAGCTATTCCAGACTGGATCAAGAGCAGAGATAATCGCTACGAGATAGATGTTTCTTACAATAATTTTTCTGTGAGCTCTGAGCCATCTTCTTGTACAGAAAACCTGAACTTGTTCAAAAGTATTTCTACAAGCGATAACTCTTTATATGCAGAGTGCCTGAAGAACTCTCCATGTCCAAAAGATCAATACTCATTGCATATCAATTGTGGTGGTAAAGCAACCACCATTGGAGGAATCAACTTTGAAGCCGACCAAGACCCAGGAAGTCCAGCACATTTCGCTCCTTCAGAGCCTAGCTGGGGATTCAGTAGCACGGGACATTTCTGGAGTGTTAACACCACCTCTAAAGACTATATAGCAAATAATATATCTATACTTGGGATGAACAACTCTGAGTTGTACACAGATGCACGCCTCTCTCCTCTTTCTCTAACATATTATGGAAGGTGCTTTGGCAGTGGAAATTATACTGTGAAGCTTTACTTTTCGGAGATTATAATTAGAGGCAATAAATCTTTTTCCAGTCTTGGAAGGCGGATCTTTGATATTTATATTCAGGAGAAGCTAGTGTTGAAGGATTTTGACATTGAAAAGGAAGCCCAAGGGGTTGATAAGCCAGTCATCAAGGAATTTACAGCAGTTGAGGTCAAGAAGAAAACTTTAGAGATCCGATTTCATTGGTCCGGGAAAGGGACAACAGCTTCCCCAAAGAGAGGAGTATATGGTCCCCTTATTTCAGCTATCTCTATAAAATCAGAGTTCAAGCCTCCTCATGATAGCAAGACATGGGCATTTATTGTGGTGGGAGCTTCGGTTTTGTGCCTCATTCTCTTGATTTTGAGCATTCTTTGGTGGAGAGGCTGTCTAGATAAGAAGACATCAAGAGAAGAAGTTCTGAGAGGACTAGATCTGCAAACTGGCTTTTTCACCTTCAAGCAAATAAAAGCTGCCACTAATAACTTTGATCCTGTCAACAAAATTGGGGAAGGTGGTTTTGGATCTGTGTTCAAGGGCATTTTACTGGATGGTACTATAATTGCAGTAAAGCAATTATCTGCAAAATCAAAGCAAGGAAATCGTGAATTTGTGAACGAAATAGGCATGATTTCTGGCTTGCAGCATCCAAATGTCGTGAGATTGTATGGATGTTGTATTGAAGCAAATCAATTACTTTTGGTATATGAATACATGGAAAACAATAGCCTTGCACATGCTTTGTTTGGTCCGGAGAGAGGTCCACTCAAATTGGACTGGCCTACGAGGCAGAAAATATGCATAGGAATAGCAAAGGGTCTGGCATTTTTGCATGACGAATCAGCACTGAAGGTTGTACACAGAGATATTAAAGCTTCGAATGTATTACTTGACCAAGACCTTAATGCTAAGATCTCAGACTTTGGTCTGGCCAAGCTTGACGAAGAAGACAACACCCACATTAGCACCAGAGTTGCTGGAACAATAGGATACATGGCACCAGAATATGCTCTATGGGGTTATTTAACGTACAAAGCAGATGTATATAGTTTTGGTGTTGTCGCATTGGAAATTGTTGTTGGAAAGAACAACATGAAATTTCGAGCAGATGAGAACTTTGTATGCGTTGTGGATTGGGTAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0002682 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009814 GO:0009861 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0031347 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

886

Amino Acids

97.73

Weight (kDa)

6.36

Isoelectric Point (pI)

32.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	159 - 337	1.8e-11	Leucine-rich repeat region
LRR_8	PF13855	190 - 245	6.8e-06	Leucine rich repeat
LRR_8	PF13855	285 - 344	1.7e-06	Leucine rich repeat
Malectin	PF11721	410 - 594	4.3e-37	Malectin domain
Pkinase	PF00069	670 - 828	6.9e-35	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	671 - 824	8.8e-35	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000134)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g06801 FvH4_3g06801 FvH4_3g06810 FvH4_3g06830 FvH4_3g06840 FvH4_3g06840 FvH4_3g06840 FvH4_3g06860 FvH4_3g06860 FvH4_3g06900 FvH4_3g06920 FvH4_3g06940 FvH4_3g06960 FvH4_3g06991 FvH4_3g06991 FvH4_3g07041 FvH4_3g07042 FvH4_3g07044 FvH4_3g07044 FvH4_3g07044 FvH4_3g07044 FvH4_3g20230 FvH4_3g20240 FvH4_3g20240 FvH4_3g20240 FvH4_3g20240 FvH4_4g01431 FvH4_4g01431 FvH4_4g01520
malus_domestica	MD00G1006500.v1.1 MD00G1006600.v1.1 MD00G1186000.v1.1 MD05G1294300.v1.1 MD05G1294500.v1.1 MD05G1294700.v1.1 MD05G1294800.v1.1 MD05G1295100.v1.1 MD10G1272300.v1.1 MD10G1272800.v1.1 MD10G1273000.v1.1 MD10G1273200.v1.1 MD10G1273300.v1.1
prunus_persica	Prupe.4G069300_v2.0.a1 Prupe.4G069400_v2.0.a1 Prupe.4G069400_v2.0.a1 Prupe.4G069500_v2.0.a1 Prupe.4G069600_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069800_v2.0.a1
pyrus_communis	pycom05g27150 pycom10g22770 pycom10g22780 pycom10g22790 pycom10g22800 pycom11g23550 pycom11g23590
rosa_chinensis	RchiOBHm_Chr1g0336311 RchiOBHm_Chr3g0460871 RchiOBHm_Chr5g0012161 RchiOBHm_Chr5g0012181 RchiOBHm_Chr5g0012191 RchiOBHm_Chr5g0012201 RchiOBHm_Chr5g0012221 RchiOBHm_Chr5g0012241 RchiOBHm_Chr5g0012251 RchiOBHm_Chr5g0012261 RchiOBHm_Chr5g0012271 RchiOBHm_Chr5g0012291 RchiOBHm_Chr5g0012301 RchiOBHm_Chr5g0012321 RchiOBHm_Chr5g0034051 RchiOBHm_Chr5g0034071 RchiOBHm_Chr5g0034091 RchiOBHm_Chr5g0080321 RchiOBHm_Chr7g0226661
rosa_laevigata	RLG00000001709 RLG00000003039 RLG00000009208 RLG00000021256 RLG00000031886 RLG00000031891 RLG00000031892 RLG00000031895 RLG00000031896 RLG00000031897 RLG00000031898 RLG00000031899 RLG00000031900 RLG00000031903 RLG00000033520 RLG00000033522 RLG00000033524 RLG00000033525
rosa_multiflora	Rmu_co8172290.1_g000001 Rmu_co8214942.1_g000001 Rmu_co8328697.1_g000001 Rmu_sc0001608.1_g000041 Rmu_sc0003368.1_g000024 Rmu_sc0003701.1_g000002 Rmu_sc0003765.1_g000052 Rmu_sc0005343.1_g000007 Rmu_sc0005343.1_g000008 Rmu_sc0006603.1_g000004 Rmu_sc0007030.1_g000001 Rmu_sc0007030.1_g000006 Rmu_sc0007176.1_g000013 Rmu_sc0009761.1_g000006 Rmu_sc0009761.1_g000007 Rmu_sc0012149.1_g000012 Rmu_sc0013327.1_g000001 Rmu_sc0013327.1_g000003 Rmu_sc0014215.1_g000004 Rmu_ssc0000128.1_g000005 Rmu_ssc0000128.1_g000012 Rmu_ssc0000128.1_g000016 Rmu_ssc0000217.1_g000012 Rmu_ssc0000217.1_g000014 Rmu_ssc0000217.1_g000018 Rmu_ssc0000217.1_g000020 Rmu_ssc0000217.1_g000026 Rmu_ssc0000217.1_g000037
rosa_roxburghii	Rroxscaffold_1G00014960 Rroxscaffold_1G00046140 Rroxscaffold_1G00046190 Rroxscaffold_1G00064330 Rroxscaffold_1G00064370 Rroxscaffold_1G00064380 Rroxscaffold_1G00064390 Rroxscaffold_1G00064400 Rroxscaffold_1G00064420 Rroxscaffold_1G00064430 Rroxscaffold_1G00064460 Rroxscaffold_1G00064470 Rroxscaffold_1G00064480 Rroxscaffold_2G00092560 Rroxscaffold_3G00232520 Rroxscaffold_3G00236850 Rroxscaffold_4G00292720 Rroxscaffold_4G00315770 Rroxscaffold_4G00315780 Rroxscaffold_4G00315790
rosa_rugosa	Rorug01G0123800 Rorug01G0123900 Rorug01G0124000 Rorug01G0124000 Rorug03G0284000 Rorug04G0090200 Rorug05G0000200 Rorug05G0000200 Rorug05G0000300 Rorug05G0000300 Rorug05G0000300 Rorug05G0000300 Rorug05G0000400 Rorug05G0000600 Rorug05G0144300 Rorug05G0144300
rosa_samantha	Rh1CG135900 Rh2DG334300 Rh2DG334500 Rh5AG090300 Rh5AG093400 Rh5AG093500 Rh5AG093900 Rh5AG094000 Rh5AG094100 Rh5AG094200 Rh5AG094400 Rh5AG094600 Rh5AG100500 Rh5AG235400 Rh5AG235500 Rh5BG089400 Rh5BG412100 Rh5CG103100 Rh5CG103800 Rh5CG103900 Rh6AG058500 Rh6BG478600 Rh6CG484600 Rh7AG387000
rosa_wichuraiana	Rw0G004700 Rw1G011820 Rw5G007880 Rw5G008160 Rw5G008170 Rw5G008180 Rw5G008200 Rw5G008210 Rw5G008220 Rw5G008230 Rw5G008240 Rw5G008260 Rw5G021250 Rw5G021260 Rw5G021390 Rw5G021400 Rw5G021410 Rw5G021420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1535
AccB1I	GGYRCC	1 cut(s)	2509
AccBSI	CCGCTC	1 cut(s)	623
AccI	GTMKAC	4 cut(s)	95, 177, 419, 745
AccIII	TCCGGA	2 cut(s)	876, 2263
AciI	CCGC	3 cut(s)	404, 621, 1573
AclWI	GGATC	7 cut(s)	724, 809, 1061, 1583, 1699, 1997, 2044
AcoI	YGGCCR	2 cut(s)	618, 2442
AcsI	RAATTY	3 cut(s)	1667, 2120, 2609
AcuI	CTGAAG	5 cut(s)	96, 701, 1217, 1308, 2381
AcyI	GRCGYC	1 cut(s)	416
AfaI	GTAC	5 cut(s)	1132, 1439, 2068, 2370, 2543
AfiI	CCNNNNNNNGG	6 cut(s)	322, 554, 1337, 1728, 1751, 2270
AflIII	ACRYGT	1 cut(s)	525
AjiI	CACGTC	1 cut(s)	286
AjnI	CCWGG	3 cut(s)	224, 480, 1297
AjuI	GAANNNNNNNTTGG	6 cut(s)	145, 177, 2016, 2048, 2574, 2606
Alw21I	GWGCWC	1 cut(s)	1115
Alw26I	GTCTC	1 cut(s)	605
AlwI	GGATC	7 cut(s)	724, 809, 1061, 1583, 1699, 1997, 2044
Aor13HI	TCCGGA	2 cut(s)	876, 2263
AoxI	GGCC	6 cut(s)	204, 227, 618, 751, 2291, 2442
ApaI	GGGCCC	1 cut(s)	755
ApeKI	GCWGC	7 cut(s)	140, 186, 221, 396, 663, 1985, 2155
ApoI	RAATTY	3 cut(s)	1667, 2120, 2609
AseI	ATTAAT	1 cut(s)	869
Asp700I	GAANNNNTTC	4 cut(s)	386, 490, 1148, 1929
AspS9I	GGNCC	9 cut(s)	204, 464, 751, 752, 896, 1719, 1763, 2261, 2273
AsuC2I	CCSGG	1 cut(s)	1723
AsuHPI	GGTGA	2 cut(s)	241, 1957
AsuII	TTCGAA	1 cut(s)	2390
AvaII	GGWCC	6 cut(s)	464, 896, 1719, 1763, 2261, 2273
BaeGI	GKGCMC	1 cut(s)	755
BalI	TGGCCA	1 cut(s)	2444
BanI	GGYRCC	1 cut(s)	2509
BanII	GRGCYC	2 cut(s)	755, 1115
BauI	CACGAG	1 cut(s)	376
BbsI	GAAGAC	2 cut(s)	1919, 2466
Bbv12I	GWGCWC	1 cut(s)	1115
BbvI	GCAGC	7 cut(s)	127, 198, 208, 408, 675, 1972, 2167
BccI	CCATC	4 cut(s)	266, 769, 1129, 2057
BcgI	CGANNNNNNTGC	4 cut(s)	409, 443, 2096, 2130
BciT130I	CCWGG	3 cut(s)	226, 482, 1299
BciVI	GTATCC	1 cut(s)	2495
BclI	TGATCA	1 cut(s)	360
BcnI	CCSGG	1 cut(s)	1723
BcoDI	GTCTC	1 cut(s)	605
BfaI	CTAG	6 cut(s)	291, 738, 1332, 1604, 1904, 1943
BfmI	CTRYAG	1 cut(s)	746
BfuI	GTATCC	1 cut(s)	2495
BglI	GCCNNNNNGGC	1 cut(s)	2299
BglII	AGATCT	3 cut(s)	943, 1945, 2424
BisI	GCNGC	8 cut(s)	141, 187, 222, 397, 621, 664, 1986, 2156
BlsI	GCNGC	8 cut(s)	142, 188, 223, 398, 622, 665, 1987, 2157
Bme1390I	CCNGG	4 cut(s)	226, 482, 1299, 1723
Bme18I	GGWCC	6 cut(s)	464, 896, 1719, 1763, 2261, 2273
BmgBI	CACGTC	1 cut(s)	286
BmgT120I	GGNCC	9 cut(s)	204, 464, 751, 752, 896, 1719, 1763, 2261, 2273
BmiI	GGNNCC	4 cut(s)	753, 754, 1765, 2511
BmrFI	CCNGG	4 cut(s)	226, 482, 1299, 1723
BmsI	GCATC	4 cut(s)	21, 303, 1435, 2167
BoxI	GACNNNNGTC	1 cut(s)	2436
BpiI	GAAGAC	2 cut(s)	1919, 2466
BplI	GAGNNNNNCTC	2 cut(s)	2262, 2294
BpmI	CTGGAG	1 cut(s)	1384
Bpu10I	CCTNAGC	1 cut(s)	549
Bpu14I	TTCGAA	1 cut(s)	2390
BpuEI	CTTGAG	2 cut(s)	799, 862
BpuMI	CCSGG	1 cut(s)	1723
BsaHI	GRCGYC	1 cut(s)	416
BsaJI	CCNNGG	3 cut(s)	481, 1297, 1639
BsaWI	WCCGGW	3 cut(s)	876, 1034, 2263
BsaXI	ACNNNNNCTCC	4 cut(s)	1836, 1866, 1885, 1915
Bsc4I	CCNNNNNNNGG	6 cut(s)	322, 554, 1337, 1728, 1751, 2270
Bse118I	RCCGGY	1 cut(s)	616
Bse1I	ACTGG	7 cut(s)	682, 1055, 1560, 1655, 1960, 2064, 2294
Bse3DI	GCAATG	2 cut(s)	196, 1229
BseAI	TCCGGA	2 cut(s)	876, 2263
BseBI	CCWGG	3 cut(s)	226, 482, 1299
BseDI	CCNNGG	3 cut(s)	481, 1297, 1639
BseGI	GGATG	4 cut(s)	1425, 2068, 2158, 2189
BseLI	CCNNNNNNNGG	6 cut(s)	322, 554, 1337, 1728, 1751, 2270
BseMI	GCAATG	2 cut(s)	196, 1229
BseMII	CTCAG	9 cut(s)	500, 540, 546, 644, 788, 1107, 1422, 1925, 2442
BseNI	ACTGG	7 cut(s)	682, 1055, 1560, 1655, 1960, 2064, 2294
BseRI	GAGGAG	3 cut(s)	1449, 1767, 1797
BseSI	GKGCMC	1 cut(s)	755
BseX3I	CGGCCG	1 cut(s)	618
BseXI	GCAGC	7 cut(s)	127, 198, 208, 408, 675, 1972, 2167
BseYI	CCCAGC	3 cut(s)	201, 320, 1335
BsgI	GTGCAG	1 cut(s)	126
Bsh1285I	CGRYCG	1 cut(s)	621
BshFI	GGCC	6 cut(s)	206, 229, 620, 753, 2293, 2444
BshNI	GGYRCC	1 cut(s)	2509
BsiEI	CGRYCG	1 cut(s)	621
BsiHKAI	GWGCWC	1 cut(s)	1115
BsiSI	CCGG	5 cut(s)	617, 877, 1035, 1722, 2264
BslFI	GGGAC	3 cut(s)	1368, 1744, 1749
BslI	CCNNNNNNNGG	6 cut(s)	322, 554, 1337, 1728, 1751, 2270
BsmAI	GTCTC	1 cut(s)	605
BsmFI	GGGAC	3 cut(s)	1368, 1744, 1749
BsmI	GAATGC	3 cut(s)	690, 972, 1881
BsnI	GGCC	6 cut(s)	206, 229, 620, 753, 2293, 2444
Bsp119I	TTCGAA	1 cut(s)	2390
Bsp120I	GGGCCC	1 cut(s)	751
Bsp1286I	GDGCHC	2 cut(s)	755, 1115
Bsp13I	TCCGGA	2 cut(s)	876, 2263
Bsp1407I	TGTACA	3 cut(s)	1130, 1437, 2368
BspACI	CCGC	3 cut(s)	404, 621, 1573
BspANI	GGCC	6 cut(s)	206, 229, 620, 753, 2293, 2444
BspCNI	CTCAG	9 cut(s)	501, 541, 545, 645, 789, 1108, 1423, 1926, 2441
BspEI	TCCGGA	2 cut(s)	876, 2263
BspHI	TCATGA	2 cut(s)	27, 1810
BspLI	GGNNCC	4 cut(s)	753, 754, 1765, 2511
BspPI	GGATC	7 cut(s)	724, 809, 1061, 1583, 1699, 1997, 2044
BspT104I	TTCGAA	1 cut(s)	2390
BspT107I	GGYRCC	1 cut(s)	2509
BsrBI	CCGCTC	1 cut(s)	623
BsrDI	GCAATG	2 cut(s)	196, 1229
BsrFI	RCCGGY	1 cut(s)	616
BsrGI	TGTACA	3 cut(s)	1130, 1437, 2368
BsrI	ACTGG	7 cut(s)	682, 1055, 1560, 1655, 1960, 2064, 2294
BssAI	RCCGGY	1 cut(s)	616
BssECI	CCNNGG	3 cut(s)	481, 1297, 1639
BssNI	GRCGYC	1 cut(s)	416
BssSI	CACGAG	1 cut(s)	376
BssT1I	CCWWGG	1 cut(s)	1639
Bst2BI	CACGAG	1 cut(s)	376
Bst2UI	CCWGG	3 cut(s)	226, 482, 1299
Bst4CI	ACNGT	7 cut(s)	93, 247, 336, 448, 599, 930, 1510
BstACI	GRCGYC	1 cut(s)	416
BstAUI	TGTACA	3 cut(s)	1130, 1437, 2368
BstBI	TTCGAA	1 cut(s)	2390
BstC8I	GCNNGC	4 cut(s)	280, 618, 1450, 2153
BstF5I	GGATG	4 cut(s)	1425, 2068, 2158, 2189
BstMAI	GTCTC	1 cut(s)	605
BstMCI	CGRYCG	1 cut(s)	621
BstMWI	GCNNNNNNNGC	4 cut(s)	63, 86, 1316, 2299
BstNI	CCWGG	3 cut(s)	226, 482, 1299
BstNSI	RCATGY	4 cut(s)	66, 282, 529, 2252
BstPAI	GACNNNNGTC	1 cut(s)	2436
BstSCI	CCNGG	4 cut(s)	224, 480, 1297, 1721
BstSFI	CTRYAG	1 cut(s)	746
BstSLI	GKGCMC	1 cut(s)	755
BstV1I	GCAGC	7 cut(s)	127, 198, 208, 408, 675, 1972, 2167
BstV2I	GAAGAC	2 cut(s)	1919, 2466
BstX2I	RGATCY	7 cut(s)	729, 943, 1575, 1704, 1945, 2036, 2424
BstXI	CCANNNNNNTGG	1 cut(s)	740
BstYI	RGATCY	7 cut(s)	729, 943, 1575, 1704, 1945, 2036, 2424
BstZI	CGGCCG	1 cut(s)	618
BsuI	GTATCC	1 cut(s)	2495
BsuRI	GGCC	6 cut(s)	206, 229, 620, 753, 2293, 2444
BtrI	CACGTC	1 cut(s)	286
BtsCI	GGATG	4 cut(s)	1425, 2068, 2158, 2189
BtsI	GCAGTG	1 cut(s)	1501
BtsIMutI	CAGTG	3 cut(s)	675, 1501, 2357
Cac8I	GCNNGC	4 cut(s)	280, 618, 1450, 2153
CciI	TCATGA	2 cut(s)	27, 1810
Cfr10I	RCCGGY	1 cut(s)	616
Cfr13I	GGNCC	9 cut(s)	204, 464, 751, 752, 896, 1719, 1763, 2261, 2273
CseI	GACGC	2 cut(s)	163, 405
Csp6I	GTAC	5 cut(s)	1131, 1438, 2067, 2369, 2542
CspCI	CAANNNNNGTGG	4 cut(s)	1228, 1263, 2265, 2300
CviQI	GTAC	5 cut(s)	1131, 1438, 2067, 2369, 2542
EaeI	YGGCCR	2 cut(s)	618, 2442
EagI	CGGCCG	1 cut(s)	618
EciI	GGCGGA	1 cut(s)	1588
Ecl136II	GAGCTC	1 cut(s)	1113
EclXI	CGGCCG	1 cut(s)	618
Eco130I	CCWWGG	1 cut(s)	1639
Eco147I	AGGCCT	1 cut(s)	229
Eco24I	GRGCYC	2 cut(s)	755, 1115
Eco47I	GGWCC	6 cut(s)	464, 896, 1719, 1763, 2261, 2273
Eco52I	CGGCCG	1 cut(s)	618
Eco53kI	GAGCTC	1 cut(s)	1113
Eco57I	CTGAAG	5 cut(s)	96, 701, 1217, 1308, 2381
EcoICRI	GAGCTC	1 cut(s)	1113
EcoO109I	RGGNCCY	2 cut(s)	751, 752
EcoRII	CCWGG	3 cut(s)	224, 480, 1297
EcoT14I	CCWWGG	1 cut(s)	1639
EcoT22I	ATGCAT	1 cut(s)	2315
EcoT38I	GRGCYC	2 cut(s)	755, 1115
ErhI	CCWWGG	1 cut(s)	1639
FalI	AAGNNNNNCTT	2 cut(s)	1505, 1537
FaqI	GGGAC	3 cut(s)	1368, 1744, 1749
FauNDI	CATATG	1 cut(s)	849
FbaI	TGATCA	1 cut(s)	360
FblI	GTMKAC	4 cut(s)	95, 177, 419, 745
Fnu4HI	GCNGC	8 cut(s)	141, 187, 222, 397, 621, 664, 1986, 2156
FokI	GGATG	4 cut(s)	1432, 2075, 2145, 2196
FriOI	GRGCYC	2 cut(s)	755, 1115
Fsp4HI	GCNGC	8 cut(s)	141, 187, 222, 397, 621, 664, 1986, 2156
FspBI	CTAG	6 cut(s)	291, 738, 1332, 1604, 1904, 1943
GluI	GCNGC	8 cut(s)	141, 187, 222, 397, 621, 664, 1986, 2156
GsaI	CCCAGC	3 cut(s)	205, 324, 1339
GsuI	CTGGAG	1 cut(s)	1384
HaeIII	GGCC	6 cut(s)	206, 229, 620, 753, 2293, 2444
HapII	CCGG	5 cut(s)	617, 877, 1035, 1722, 2264
HgaI	GACGC	2 cut(s)	163, 405
Hin1I	GRCGYC	1 cut(s)	416
HincII	GTYRAC	7 cut(s)	178, 420, 451, 568, 645, 1372, 2011
HindII	GTYRAC	7 cut(s)	178, 420, 451, 568, 645, 1372, 2011
HindIII	AAGCTT	4 cut(s)	949, 1514, 2385, 2447
HinfI	GANTC	8 cut(s)	577, 631, 692, 709, 844, 1272, 1341, 2351
HpaI	GTTAAC	3 cut(s)	568, 645, 1372
HpaII	CCGG	5 cut(s)	617, 877, 1035, 1722, 2264
HphI	GGTGA	2 cut(s)	241, 1957
Hpy99I	CGWCG	2 cut(s)	179, 421
HpyAV	CCTTC	9 cut(s)	274, 915, 1332, 1476, 1563, 1606, 1978, 2018, 2356
HpyCH4III	ACNGT	7 cut(s)	93, 247, 336, 448, 599, 930, 1510
HpyCH4IV	ACGT	2 cut(s)	285, 2540
HpyF10VI	GCNNNNNNNGC	4 cut(s)	63, 86, 1316, 2299
HpySE526I	ACGT	2 cut(s)	285, 2540
Hsp92I	GRCGYC	1 cut(s)	416
Kpn2I	TCCGGA	2 cut(s)	876, 2263
KroI	GCCGGC	1 cut(s)	616
KroNI	GCCGGC	1 cut(s)	618
Ksp22I	TGATCA	1 cut(s)	360
KspAI	GTTAAC	3 cut(s)	568, 645, 1372
LmnI	GCTCC	7 cut(s)	266, 879, 879, 969, 1037, 1324, 1844
Lsp1109I	GCAGC	7 cut(s)	127, 198, 208, 408, 675, 1972, 2167
LweI	GCATC	4 cut(s)	21, 303, 1435, 2167
MaeI	CTAG	6 cut(s)	291, 738, 1332, 1604, 1904, 1943
MaeII	ACGT	2 cut(s)	285, 2540
MaeIII	GTNAC	6 cut(s)	247, 254, 286, 348, 884, 924
MbiI	CCGCTC	1 cut(s)	623
MfeI	CAATTG	1 cut(s)	1240
MflI	RGATCY	7 cut(s)	729, 943, 1575, 1704, 1945, 2036, 2424
MhlI	GDGCHC	2 cut(s)	755, 1115
MlsI	TGGCCA	1 cut(s)	2444
MluNI	TGGCCA	1 cut(s)	2444
MlyI	GAGTC	2 cut(s)	686, 853
MmeI	TCCRAC	3 cut(s)	497, 686, 2572
Mox20I	TGGCCA	1 cut(s)	2444
Mph1103I	ATGCAT	1 cut(s)	2315
MroI	TCCGGA	2 cut(s)	876, 2263
MroNI	GCCGGC	1 cut(s)	616
MroXI	GAANNNNTTC	4 cut(s)	386, 490, 1148, 1929
MscI	TGGCCA	1 cut(s)	2444
MseI	TTAA	9 cut(s)	303, 567, 644, 812, 869, 1371, 2382, 2415, 2537
MslI	CAYNNNNRTG	1 cut(s)	2163
Msp20I	TGGCCA	1 cut(s)	2444
MspI	CCGG	5 cut(s)	617, 877, 1035, 1722, 2264
MspR9I	CCNGG	4 cut(s)	226, 482, 1299, 1723
MunI	CAATTG	1 cut(s)	1240
Mva1269I	GAATGC	3 cut(s)	690, 972, 1881
MvaI	CCWGG	3 cut(s)	226, 482, 1299
MwoI	GCNNNNNNNGC	4 cut(s)	63, 86, 1316, 2299
NaeI	GCCGGC	1 cut(s)	618
NciI	CCSGG	1 cut(s)	1723
NdeI	CATATG	1 cut(s)	849
NgoMIV	GCCGGC	1 cut(s)	616
NlaIV	GGNNCC	4 cut(s)	753, 754, 1765, 2511
NmuCI	GTSAC	3 cut(s)	247, 286, 924
NsiI	ATGCAT	1 cut(s)	2315
NspI	RCATGY	4 cut(s)	66, 282, 529, 2252
NspV	TTCGAA	1 cut(s)	2390
PaeI	GCATGC	1 cut(s)	282
PagI	TCATGA	2 cut(s)	27, 1810
PceI	AGGCCT	1 cut(s)	229
PciI	ACATGT	1 cut(s)	525
PctI	GAATGC	3 cut(s)	690, 972, 1881
PdiI	GCCGGC	1 cut(s)	618
PdmI	GAANNNNTTC	4 cut(s)	386, 490, 1148, 1929
PfeI	GAWTC	6 cut(s)	577, 631, 709, 1272, 1341, 2351
PfoI	TCCNGGA	1 cut(s)	1721
PkrI	GCNGC	8 cut(s)	142, 188, 223, 398, 622, 665, 1987, 2157
PleI	GAGTC	2 cut(s)	686, 852
PpsI	GAGTC	2 cut(s)	686, 852
PscI	ACATGT	1 cut(s)	525
PshAI	GACNNNNGTC	1 cut(s)	2436
PshBI	ATTAAT	1 cut(s)	869
PsiI	TTATAA	1 cut(s)	1535
Psp124BI	GAGCTC	1 cut(s)	1115
Psp6I	CCWGG	3 cut(s)	224, 480, 1297
PspFI	CCCAGC	3 cut(s)	201, 320, 1335
PspGI	CCWGG	3 cut(s)	224, 480, 1297
PspN4I	GGNNCC	4 cut(s)	753, 754, 1765, 2511
PspOMI	GGGCCC	1 cut(s)	751
PspPI	GGNCC	9 cut(s)	204, 464, 751, 752, 896, 1719, 1763, 2261, 2273
PsuI	RGATCY	7 cut(s)	729, 943, 1575, 1704, 1945, 2036, 2424
RsaI	GTAC	5 cut(s)	1132, 1439, 2068, 2370, 2543
RsaNI	GTAC	5 cut(s)	1131, 1438, 2067, 2369, 2542
RseI	CAYNNNNRTG	1 cut(s)	2163
SacI	GAGCTC	1 cut(s)	1115
SalI	GTCGAC	2 cut(s)	176, 418
SaqAI	TTAA	9 cut(s)	303, 567, 644, 812, 869, 1371, 2382, 2415, 2537
SatI	GCNGC	8 cut(s)	141, 187, 222, 397, 621, 664, 1986, 2156
Sau96I	GGNCC	9 cut(s)	204, 464, 751, 752, 896, 1719, 1763, 2261, 2273
SchI	GAGTC	2 cut(s)	686, 853
ScrFI	CCNGG	4 cut(s)	226, 482, 1299, 1723
SduI	GDGCHC	2 cut(s)	755, 1115
SfaNI	GCATC	4 cut(s)	21, 303, 1435, 2167
SfcI	CTRYAG	1 cut(s)	746
SfuI	TTCGAA	1 cut(s)	2390
SinI	GGWCC	6 cut(s)	464, 896, 1719, 1763, 2261, 2273
SmiMI	CAYNNNNRTG	1 cut(s)	2163
SmlI	CTYRAG	2 cut(s)	778, 841
SmoI	CTYRAG	2 cut(s)	778, 841
SphI	GCATGC	1 cut(s)	282
SseBI	AGGCCT	1 cut(s)	229
SsiI	CCGC	3 cut(s)	404, 621, 1573
SspI	AATATT	2 cut(s)	42, 939
SspMI	CTAG	6 cut(s)	291, 738, 1332, 1604, 1904, 1943
SstI	GAGCTC	1 cut(s)	1115
StuI	AGGCCT	1 cut(s)	229
StyD4I	CCNGG	4 cut(s)	224, 480, 1297, 1721
StyI	CCWWGG	1 cut(s)	1639
TaaI	ACNGT	7 cut(s)	93, 247, 336, 448, 599, 930, 1510
TaiI	ACGT	2 cut(s)	288, 2543
TaqI	TCGA	5 cut(s)	177, 369, 419, 2390, 2614
TatI	WGTACW	3 cut(s)	1130, 1437, 2368
TauI	GCSGC	1 cut(s)	623
TfiI	GAWTC	6 cut(s)	577, 631, 709, 1272, 1341, 2351
Tru1I	TTAA	9 cut(s)	303, 567, 644, 812, 869, 1371, 2382, 2415, 2537
Tru9I	TTAA	9 cut(s)	303, 567, 644, 812, 869, 1371, 2382, 2415, 2537
TscAI	CASTG	3 cut(s)	682, 1501, 2364
TseFI	GTSAC	3 cut(s)	247, 286, 924
TseI	GCWGC	7 cut(s)	140, 186, 221, 396, 663, 1985, 2155
Tsp45I	GTSAC	3 cut(s)	247, 286, 924
TspDTI	ATGAA	6 cut(s)	17, 644, 1436, 1703, 2235, 2621
TspGWI	ACGGA	1 cut(s)	831
TspRI	CASTG	3 cut(s)	682, 1501, 2364
VpaK11BI	GGWCC	6 cut(s)	464, 896, 1719, 1763, 2261, 2273
VspI	ATTAAT	1 cut(s)	869
XapI	RAATTY	3 cut(s)	1667, 2120, 2609
XbaI	TCTAGA	1 cut(s)	1903
XceI	RCATGY	4 cut(s)	66, 282, 529, 2252
XmiI	GTMKAC	4 cut(s)	95, 177, 419, 745
XmnI	GAANNNNTTC	4 cut(s)	386, 490, 1148, 1929
XspI	CTAG	6 cut(s)	291, 738, 1332, 1604, 1904, 1943
Zsp2I	ATGCAT	1 cut(s)	2315

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.