Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000134

Physical Location & Seq

Genomic Coordinates Reverse (-)

36509425 .. 36522844

Gene Structure 13420 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1273300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3012 bp

ATGCTCACATTGATCTTTTGCTTCTCCTCATCATCAACAGTCAAAATTGAAGCACAGTCTCAATCTGATCCTAAAGAAGTGGAAACACTAAAGGAAATAGCTAAACAAATTGGGAAAAAAGACTGGAACTTCAGCATAGACCCATGCATCAATGACACAAATTGGGCTACCCCAAAATCTGCTAACTTGCCTCACTACAACAACACTGTCATCTGCAACTGCTCCACGCCTGATGGTTTTTGCCATGTTGTCAGCATTTTTCTCATGGGGCAAGATCTTGCTGGTGTAGTTCCACCATCAATTGCAAAGTTACCCTACCTAAAGAAAGTTGATTTCTCCCAGAACTACCTCAGCGGTACTATACCGCGTGAATGGGCTTCTACGAAGTTGGAAGTTCTGTCCATTAATGTGAACAACTTATCGGGACCGATCCCTGGCTACTTGGGATACATTACCAGCCTAAAATATATGAACTTACAGAATAACATGTTGTCTGGAACTGTTCCTCATGAGCTTGGGAAATTGGTTAACTTGAACAATCTCATTCTGAATGAAAACTATCTGACGGGAGAGCTGCCTGGGTCTCTTACTAAGCTGACTGAGTTAACAGAACTTAGAATTAGCAGTAACAGCTTCACTGGAAAAATACCTGATTATTTTCAAAGCTGGAAGCATCTTCAGAAACTAGAGATCCAAGCTAGTGGCTTCCAAGGTCCAATTCCATCGAGCATTTCCGTCTTAAGTAATTTAAAAGAACTAAGGATCAGTGACATAAAAAGTGGTGGTTCAAAATTTCCACCCTTGAGTCGTATGACAAACATGCAACGTTTAATGCTGAGGAGGTGTAACTTATATGGACCTATCCCCGCTTATATGGCAGCATTGACAAACTTGAAAATATTAGATCTCAGTTTCAATAGATTGGAAGGAAATATTCCTGATATGGCAGCTCTGTCAAACTTGCAGTGGATATATCTCACAAGCAACTTGCTCACTGGGTCTATTCCAGACTGGATCAAGCTCAAAAATACTAGAACCCAGATAGATCTTTCTTACAATAATTTTTCTCAGACCTCCGAGCCAACTACTTGTAGAGAACAGTTGAACTTGTTCAAAAGCTTTTCTGGACGAGACAACTCATTATCTGGAGGGTGCCTCGAAACTTTTTCGTGTCCAAAAGAGGAGTACTCATTACATATTAACTGTGGAGGAAGTAAAACCACCATTGGAGGAATTATATTTGAAGAGGATCAAGACCTGGGGGGTGATGCAAAATTTGTTCCGGTGATACCTAACTGGGGAATCAGTTCCACTGGATTGTTTTGGGATGTTAACACCACGGCTTATGACTACATAGCAAATAATATGTCCATACTTGAAATGAACAACTCGGAGTTGTACACGAGCGCACGCCTATCGCCTCTTTCTCTCACATATTATGCACGCTGCCTGGGAAATGGAAATTATACCGTGAAACTCCATTTTTCAGAGATAATAATCAGAGGCAATAAATCATTTTACAGTTTTGGAAGACGGATATTTGATGTCTACATTCAGGAGAAACTAGTGTGGAAGGATTTTGACATAGAAAAGGAAGCACATGGGGTGGACAGGGTAGTCATTAAGGAACTTAAAGCAGTTCATGTTAAGGATAGAACTTTACTGATCCGGTTTCATTGGTCAGGGAAAGGGACAACAACCTCCCCAAAGAGAGGAACATATGGTCCTCTTATATCAGCTATCTCTGTAGACTCTGACTTCAAGCCTCCTCTCAGCAGTAGCAAAAGCAAGATGCCTATTGTGGTTGGAACTGCAGTTGGAGCTTCGGTTGTGTGCCTCATTTTCTTGACTTTAGGCATTCTTTGGTGGAGATGTTGTCCAGATAGCATGACATCAAGGGAAAAAGCTCTAAGAGGGCTGGATCTGCAAACTGGTTTTTTCACCTTCAGGCAAATTAAAGCTGCCACTAATAACTTCGATCCAAAAAACAAAATTGGAGAAGGCGGTTTTGGGTCTGTCTACAAGGGTGTATTGGTGGATGGTACTATAATCGCGGTTAAGCAACTATCATCAAAATCAAAGCAAGGAAACCGGGAGTTTGTGAATGAAATAGGCATGATATCCGCCTTGCATCATCCAAATCTTGTTAGATTGTATGGATGTTGTATTGAATCAAACCAATTATTGTTGGTATATGAATATATGGAAAATAATAGCCTGGCACATACTTTGTTCGGCCCAGAGGAAGGTCCTTTAAAGCTGGATTGGCCTACGAGGGAGAAAATATGCATAGGTATAGCGAGAGGTCTGGCTTTTTTGCATGAGGAGTCGGCACTGAAGGTTGTACACCGAGACATTAAAACTACAAATATACTGCTAGACCAAGACCTCAACGCAAAGATCTCCGACTTCGGTTTGGCCAAACTTGACGATGAGGAGAACACCCACATTAGCACCAGAGTTGCTGGAACCATAGGATACATGGCTCCGGAATATGCACTATGGGGTTATTTGACATATAAAGCAGATGTTTACAGTTTTGGTGTGGTTGCATTGGAAATTGTTGCTGGAAAGAACAACATGAAATATCAACCAAATGAGAACTTCGTATGCCTTCTGGATTGGGCTCTTGTATTACAACAAAAGGGGAACTTAATAGAGCTAGTGGATCCAAGGTTGGGGTCCGATTTCGATGTGGAAGAGGTGATTACAATGGTTAAGGTAGCTCTCTTATGCATCAATCCAGCACCAGCACTCAGGCCTACAATGTCTGAAGTAGTGAGCATGCTCGAAGGACGAACCCCTGTTTGTGAATCGGTCATGAACCCAAGTATCTACGGCGATGAACTGAGGTTAGCATCCTTGAGAAACCCGTTTGATCCGATTGCACAGCAGAGTACAAGTGAAACTCAGAGCCTTGTTGGTTCATCAGATGCAACATTGATTCATTCTTCTGCAACTACGTCCTCAGATCTCTATAAAATTGGTCCTAGTAGTAGCACATTATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0002682 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009814 GO:0009861 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0031347 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

1004

Amino Acids

111.2

Weight (kDa)

6.5

Isoelectric Point (pI)

34.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	130 - 258	1.9e-11	Leucine-rich repeat region
Malectin	PF11721	397 - 581	4.2e-37	Malectin domain
Pkinase	PF00069	661 - 927	1.2e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	662 - 928	1.4e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0000134)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g06801 FvH4_3g06801 FvH4_3g06810 FvH4_3g06830 FvH4_3g06840 FvH4_3g06840 FvH4_3g06840 FvH4_3g06860 FvH4_3g06860 FvH4_3g06900 FvH4_3g06920 FvH4_3g06940 FvH4_3g06960 FvH4_3g06991 FvH4_3g06991 FvH4_3g07041 FvH4_3g07042 FvH4_3g07044 FvH4_3g07044 FvH4_3g07044 FvH4_3g07044 FvH4_3g20230 FvH4_3g20240 FvH4_3g20240 FvH4_3g20240 FvH4_3g20240 FvH4_4g01431 FvH4_4g01431 FvH4_4g01520
malus_domestica	MD00G1006500.v1.1 MD00G1006600.v1.1 MD00G1186000.v1.1 MD05G1294300.v1.1 MD05G1294500.v1.1 MD05G1294700.v1.1 MD05G1294800.v1.1 MD05G1295100.v1.1 MD10G1272300.v1.1 MD10G1272800.v1.1 MD10G1273000.v1.1 MD10G1273200.v1.1 MD10G1273300.v1.1
prunus_persica	Prupe.4G069300_v2.0.a1 Prupe.4G069400_v2.0.a1 Prupe.4G069400_v2.0.a1 Prupe.4G069500_v2.0.a1 Prupe.4G069600_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069800_v2.0.a1
pyrus_communis	pycom05g27150 pycom10g22770 pycom10g22780 pycom10g22790 pycom10g22800 pycom11g23550 pycom11g23590
rosa_chinensis	RchiOBHm_Chr1g0336311 RchiOBHm_Chr3g0460871 RchiOBHm_Chr5g0012161 RchiOBHm_Chr5g0012181 RchiOBHm_Chr5g0012191 RchiOBHm_Chr5g0012201 RchiOBHm_Chr5g0012221 RchiOBHm_Chr5g0012241 RchiOBHm_Chr5g0012251 RchiOBHm_Chr5g0012261 RchiOBHm_Chr5g0012271 RchiOBHm_Chr5g0012291 RchiOBHm_Chr5g0012301 RchiOBHm_Chr5g0012321 RchiOBHm_Chr5g0034051 RchiOBHm_Chr5g0034071 RchiOBHm_Chr5g0034091 RchiOBHm_Chr5g0080321 RchiOBHm_Chr7g0226661
rosa_laevigata	RLG00000001709 RLG00000003039 RLG00000009208 RLG00000021256 RLG00000031886 RLG00000031891 RLG00000031892 RLG00000031895 RLG00000031896 RLG00000031897 RLG00000031898 RLG00000031899 RLG00000031900 RLG00000031903 RLG00000033520 RLG00000033522 RLG00000033524 RLG00000033525
rosa_multiflora	Rmu_co8172290.1_g000001 Rmu_co8214942.1_g000001 Rmu_co8328697.1_g000001 Rmu_sc0001608.1_g000041 Rmu_sc0003368.1_g000024 Rmu_sc0003701.1_g000002 Rmu_sc0003765.1_g000052 Rmu_sc0005343.1_g000007 Rmu_sc0005343.1_g000008 Rmu_sc0006603.1_g000004 Rmu_sc0007030.1_g000001 Rmu_sc0007030.1_g000006 Rmu_sc0007176.1_g000013 Rmu_sc0009761.1_g000006 Rmu_sc0009761.1_g000007 Rmu_sc0012149.1_g000012 Rmu_sc0013327.1_g000001 Rmu_sc0013327.1_g000003 Rmu_sc0014215.1_g000004 Rmu_ssc0000128.1_g000005 Rmu_ssc0000128.1_g000012 Rmu_ssc0000128.1_g000016 Rmu_ssc0000217.1_g000012 Rmu_ssc0000217.1_g000014 Rmu_ssc0000217.1_g000018 Rmu_ssc0000217.1_g000020 Rmu_ssc0000217.1_g000026 Rmu_ssc0000217.1_g000037
rosa_roxburghii	Rroxscaffold_1G00014960 Rroxscaffold_1G00046140 Rroxscaffold_1G00046190 Rroxscaffold_1G00064330 Rroxscaffold_1G00064370 Rroxscaffold_1G00064380 Rroxscaffold_1G00064390 Rroxscaffold_1G00064400 Rroxscaffold_1G00064420 Rroxscaffold_1G00064430 Rroxscaffold_1G00064460 Rroxscaffold_1G00064470 Rroxscaffold_1G00064480 Rroxscaffold_2G00092560 Rroxscaffold_3G00232520 Rroxscaffold_3G00236850 Rroxscaffold_4G00292720 Rroxscaffold_4G00315770 Rroxscaffold_4G00315780 Rroxscaffold_4G00315790
rosa_rugosa	Rorug01G0123800 Rorug01G0123900 Rorug01G0124000 Rorug01G0124000 Rorug03G0284000 Rorug04G0090200 Rorug05G0000200 Rorug05G0000200 Rorug05G0000300 Rorug05G0000300 Rorug05G0000300 Rorug05G0000300 Rorug05G0000400 Rorug05G0000600 Rorug05G0144300 Rorug05G0144300
rosa_samantha	Rh1CG135900 Rh2DG334300 Rh2DG334500 Rh5AG090300 Rh5AG093400 Rh5AG093500 Rh5AG093900 Rh5AG094000 Rh5AG094100 Rh5AG094200 Rh5AG094400 Rh5AG094600 Rh5AG100500 Rh5AG235400 Rh5AG235500 Rh5BG089400 Rh5BG412100 Rh5CG103100 Rh5CG103800 Rh5CG103900 Rh6AG058500 Rh6BG478600 Rh6CG484600 Rh7AG387000
rosa_wichuraiana	Rw0G004700 Rw1G011820 Rw5G007880 Rw5G008160 Rw5G008170 Rw5G008180 Rw5G008200 Rw5G008210 Rw5G008220 Rw5G008230 Rw5G008240 Rw5G008260 Rw5G021250 Rw5G021260 Rw5G021390 Rw5G021400 Rw5G021410 Rw5G021420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1616
AccB1I	GGYRCC	1 cut(s)	1152
AccI	GTMKAC	3 cut(s)	1548, 1749, 2019
AccII	CGCG	2 cut(s)	367, 2054
AccIII	TCCGGA	1 cut(s)	2488
AciI	CCGC	6 cut(s)	354, 365, 867, 2004, 2054, 2124
AclI	AACGTT	1 cut(s)	826
AcoI	YGGCCR	1 cut(s)	2418
AcsI	RAATTY	2 cut(s)	791, 1274
AcuI	CTGAAG	5 cut(s)	115, 662, 1930, 2357, 2793
AfaI	GTAC	6 cut(s)	358, 1187, 1400, 2044, 2346, 2899
AfiI	CCNNNNNNNGG	5 cut(s)	434, 1298, 1712, 2246, 2678
AflII	CTTAAG	1 cut(s)	739
AflIII	ACRYGT	1 cut(s)	486
AhlI	ACTAGT	1 cut(s)	1564
AjnI	CCWGG	5 cut(s)	433, 577, 1257, 1449, 2217
AjuI	GAANNNNNNNTTGG	4 cut(s)	702, 734, 1992, 2024
AloI	GAACNNNNNNTCC	2 cut(s)	1708, 1740
Alw26I	GTCTC	4 cut(s)	63, 588, 1125, 2346
Aor13HI	TCCGGA	1 cut(s)	2488
AoxI	GGCC	4 cut(s)	2236, 2267, 2418, 2759
ApeKI	GCWGC	5 cut(s)	574, 878, 947, 1446, 1961
ApoI	RAATTY	2 cut(s)	791, 1274
ArsI	GACNNNNNNTTYG	2 cut(s)	1352, 1384
AseI	ATTAAT	1 cut(s)	405
Asp700I	GAANNNNTTC	2 cut(s)	1109, 1306
AspLEI	GCGC	1 cut(s)	1409
AspS9I	GGNCC	8 cut(s)	425, 713, 857, 1724, 2237, 2249, 2682, 2987
AsuC2I	CCSGG	1 cut(s)	2093
AsuHPI	GGTGA	4 cut(s)	1277, 1297, 1933, 2716
AvaII	GGWCC	7 cut(s)	425, 713, 857, 1724, 2249, 2682, 2987
BaeI	ACNNNNGTAYC	2 cut(s)	2815, 2848
BalI	TGGCCA	1 cut(s)	2420
BamHI	GGATCC	1 cut(s)	2668
BanI	GGYRCC	1 cut(s)	1152
BanII	GRGCYC	1 cut(s)	2629
BauI	CACGAG	1 cut(s)	1402
BbsI	GAAGAC	1 cut(s)	1537
BbvCI	CCTCAGC	2 cut(s)	350, 836
BbvI	GCAGC	5 cut(s)	561, 890, 959, 1433, 1948
BccI	CCATC	4 cut(s)	227, 304, 730, 2033
BceAI	ACGGC	2 cut(s)	1356, 2854
BcgI	CGANNNNNNTGC	2 cut(s)	1398, 1432
BciT130I	CCWGG	5 cut(s)	435, 579, 1259, 1451, 2219
BciVI	GTATCC	2 cut(s)	440, 2471
BcnI	CCSGG	1 cut(s)	2093
BcoDI	GTCTC	4 cut(s)	63, 588, 1125, 2346
BcuI	ACTAGT	1 cut(s)	1564
BfaI	CTAG	7 cut(s)	686, 699, 1032, 1565, 2378, 2663, 2991
BfmI	CTRYAG	2 cut(s)	1746, 1812
BfrI	CTTAAG	1 cut(s)	739
BfuI	GTATCC	2 cut(s)	440, 2471
BglII	AGATCT	5 cut(s)	274, 904, 1045, 2400, 2971
BisI	GCNGC	5 cut(s)	575, 879, 948, 1447, 1962
BlsI	GCNGC	5 cut(s)	576, 880, 949, 1448, 1963
BmcAI	AGTACT	1 cut(s)	1187
Bme1390I	CCNGG	6 cut(s)	435, 579, 1259, 1451, 2093, 2219
Bme18I	GGWCC	7 cut(s)	425, 713, 857, 1724, 2249, 2682, 2987
BmgT120I	GGNCC	8 cut(s)	425, 713, 857, 1724, 2237, 2249, 2682, 2987
BmiI	GGNNCC	6 cut(s)	426, 1154, 2470, 2487, 2670, 2683
BmrFI	CCNGG	6 cut(s)	435, 579, 1259, 1451, 2093, 2219
BmrI	ACTGGG	2 cut(s)	1005, 1306
BmsI	GCATC	8 cut(s)	156, 682, 1258, 1782, 2140, 2745, 2867, 2923
BmuI	ACTGGG	2 cut(s)	1005, 1306
BpiI	GAAGAC	1 cut(s)	1537
BplI	GAGNNNNNCTC	4 cut(s)	1140, 1172, 1172, 1204
BpmI	CTGGAG	1 cut(s)	1167
Bpu10I	CCTNAGC	2 cut(s)	350, 836
BpuEI	CTTGAG	2 cut(s)	823, 2884
BpuMI	CCSGG	1 cut(s)	2093
BsaBI	GATNNNNATC	1 cut(s)	1496
BsaI	GGTCTC	1 cut(s)	588
BsaJI	CCNNGG	7 cut(s)	433, 578, 709, 1258, 1338, 1450, 2672
BsaWI	WCCGGW	3 cut(s)	1282, 1668, 2488
BsaXI	ACNNNNNCTCC	4 cut(s)	1812, 1842, 1861, 1891
Bsc4I	CCNNNNNNNGG	5 cut(s)	434, 1298, 1712, 2246, 2678
Bse1I	ACTGG	7 cut(s)	128, 643, 1000, 1016, 1301, 1318, 1936
Bse8I	GATNNNNATC	1 cut(s)	1496
BseAI	TCCGGA	1 cut(s)	2488
BseBI	CCWGG	5 cut(s)	435, 579, 1259, 1451, 2219
BseDI	CCNNGG	7 cut(s)	433, 578, 709, 1258, 1338, 1450, 2672
BseGI	GGATG	5 cut(s)	1333, 2044, 2134, 2165, 2858
BseJI	GATNNNNATC	1 cut(s)	1496
BseLI	CCNNNNNNNGG	5 cut(s)	434, 1298, 1712, 2246, 2678
BseNI	ACTGG	7 cut(s)	128, 643, 1000, 1016, 1301, 1318, 1936
BseRI	GAGGAG	6 cut(s)	16, 853, 1196, 1758, 2339, 2450
BseXI	GCAGC	5 cut(s)	561, 890, 959, 1433, 1948
Bsh1236I	CGCG	2 cut(s)	367, 2054
BshFI	GGCC	4 cut(s)	2238, 2269, 2420, 2761
BshNI	GGYRCC	1 cut(s)	1152
BsiSI	CCGG	4 cut(s)	1283, 1669, 2092, 2489
BslFI	GGGAC	2 cut(s)	438, 1705
BslI	CCNNNNNNNGG	5 cut(s)	434, 1298, 1712, 2246, 2678
BsmAI	GTCTC	4 cut(s)	63, 588, 1125, 2346
BsmFI	GGGAC	2 cut(s)	438, 1705
BsmI	GAATGC	1 cut(s)	1857
BsnI	GGCC	4 cut(s)	2238, 2269, 2420, 2761
Bso31I	GGTCTC	1 cut(s)	588
Bsp1286I	GDGCHC	1 cut(s)	2629
Bsp13I	TCCGGA	1 cut(s)	2488
Bsp1407I	TGTACA	2 cut(s)	1398, 2344
BspACI	CCGC	6 cut(s)	354, 365, 867, 2004, 2054, 2124
BspANI	GGCC	4 cut(s)	2238, 2269, 2420, 2761
BspEI	TCCGGA	1 cut(s)	2488
BspFNI	CGCG	2 cut(s)	367, 2054
BspHI	TCATGA	2 cut(s)	508, 2820
BspLI	GGNNCC	6 cut(s)	426, 1154, 2470, 2487, 2670, 2683
BspMAI	CTGCAG	1 cut(s)	1816
BspT107I	GGYRCC	1 cut(s)	1152
BspTI	CTTAAG	1 cut(s)	739
BspTNI	GGTCTC	1 cut(s)	588
BsrGI	TGTACA	2 cut(s)	1398, 2344
BsrI	ACTGG	7 cut(s)	128, 643, 1000, 1016, 1301, 1318, 1936
BssECI	CCNNGG	7 cut(s)	433, 578, 709, 1258, 1338, 1450, 2672
BssSI	CACGAG	1 cut(s)	1402
BssT1I	CCWWGG	2 cut(s)	709, 2672
Bst2BI	CACGAG	1 cut(s)	1402
Bst2UI	CCWGG	5 cut(s)	435, 579, 1259, 1451, 2219
Bst4CI	ACNGT	9 cut(s)	40, 57, 208, 502, 1101, 1205, 1471, 1523, 2537
Bst6I	CTCTTC	2 cut(s)	1239, 2694
BstAFI	CTTAAG	1 cut(s)	739
BstAUI	TGTACA	2 cut(s)	1398, 2344
BstC8I	GCNNGC	3 cut(s)	1411, 1444, 2786
BstDSI	CCRYGG	1 cut(s)	1338
BstF5I	GGATG	5 cut(s)	1333, 2044, 2134, 2165, 2858
BstFNI	CGCG	2 cut(s)	367, 2054
BstHHI	GCGC	1 cut(s)	1409
BstMAI	GTCTC	4 cut(s)	63, 588, 1125, 2346
BstMWI	GCNNNNNNNGC	5 cut(s)	630, 875, 1820, 1924, 2266
BstNI	CCWGG	5 cut(s)	435, 579, 1259, 1451, 2219
BstNSI	RCATGY	3 cut(s)	490, 823, 2788
BstSCI	CCNGG	6 cut(s)	433, 577, 1257, 1449, 2091, 2217
BstSFI	CTRYAG	2 cut(s)	1746, 1812
BstUI	CGCG	2 cut(s)	367, 2054
BstV1I	GCAGC	5 cut(s)	561, 890, 959, 1433, 1948
BstV2I	GAAGAC	1 cut(s)	1537
BstX2I	RGATCY	8 cut(s)	274, 690, 904, 1045, 1921, 2400, 2668, 2971
BstXI	CCANNNNNNTGG	1 cut(s)	701
BstYI	RGATCY	8 cut(s)	274, 690, 904, 1045, 1921, 2400, 2668, 2971
BsuI	GTATCC	2 cut(s)	440, 2471
BsuRI	GGCC	4 cut(s)	2238, 2269, 2420, 2761
BtgI	CCRYGG	1 cut(s)	1338
BtgZI	GCGATG	1 cut(s)	2856
BtsCI	GGATG	5 cut(s)	1333, 2044, 2134, 2165, 2858
BtsI	GCAGTG	1 cut(s)	971
BtsIMutI	CAGTG	7 cut(s)	204, 636, 772, 971, 993, 1311, 2333
Cac8I	GCNNGC	3 cut(s)	1411, 1444, 2786
CciI	TCATGA	2 cut(s)	508, 2820
CfoI	GCGC	1 cut(s)	1409
Cfr13I	GGNCC	8 cut(s)	425, 713, 857, 1724, 2237, 2249, 2682, 2987
Csp6I	GTAC	6 cut(s)	357, 1186, 1399, 2043, 2345, 2898
CviQI	GTAC	6 cut(s)	357, 1186, 1399, 2043, 2345, 2898
DraI	TTTAAA	2 cut(s)	750, 2256
DrdI	GACNNNNNNGTC	1 cut(s)	1616
DseDI	GACNNNNNNGTC	1 cut(s)	1616
EaeI	YGGCCR	1 cut(s)	2418
Eam1104I	CTCTTC	2 cut(s)	1239, 2694
EarI	CTCTTC	2 cut(s)	1239, 2694
EciI	GGCGGA	1 cut(s)	2113
Eco130I	CCWWGG	2 cut(s)	709, 2672
Eco147I	AGGCCT	1 cut(s)	2761
Eco24I	GRGCYC	1 cut(s)	2629
Eco31I	GGTCTC	1 cut(s)	588
Eco32I	GATATC	1 cut(s)	2121
Eco47I	GGWCC	7 cut(s)	425, 713, 857, 1724, 2249, 2682, 2987
Eco57I	CTGAAG	5 cut(s)	115, 662, 1930, 2357, 2793
EcoO109I	RGGNCCY	1 cut(s)	2249
EcoRII	CCWGG	5 cut(s)	433, 577, 1257, 1449, 2217
EcoRV	GATATC	1 cut(s)	2121
EcoT14I	CCWWGG	2 cut(s)	709, 2672
EcoT22I	ATGCAT	3 cut(s)	149, 2291, 2738
EcoT38I	GRGCYC	1 cut(s)	2629
ErhI	CCWWGG	2 cut(s)	709, 2672
FalI	AAGNNNNNCTT	2 cut(s)	2636, 2668
FaqI	GGGAC	2 cut(s)	438, 1705
FauI	CCCGC	1 cut(s)	874
FauNDI	CATATG	1 cut(s)	1720
FblI	GTMKAC	3 cut(s)	1548, 1749, 2019
Fnu4HI	GCNGC	5 cut(s)	575, 879, 948, 1447, 1962
FokI	GGATG	5 cut(s)	1340, 2051, 2121, 2172, 2845
FriOI	GRGCYC	1 cut(s)	2629
Fsp4HI	GCNGC	5 cut(s)	575, 879, 948, 1447, 1962
FspBI	CTAG	7 cut(s)	686, 699, 1032, 1565, 2378, 2663, 2991
GlaI	GCGC	1 cut(s)	1408
GluI	GCNGC	5 cut(s)	575, 879, 948, 1447, 1962
GsuI	CTGGAG	1 cut(s)	1167
HaeIII	GGCC	4 cut(s)	2238, 2269, 2420, 2761
HapII	CCGG	4 cut(s)	1283, 1669, 2092, 2489
HhaI	GCGC	1 cut(s)	1409
Hin6I	GCGC	1 cut(s)	1407
HinP1I	GCGC	1 cut(s)	1407
HincII	GTYRAC	3 cut(s)	529, 606, 1333
HindII	GTYRAC	3 cut(s)	529, 606, 1333
HindIII	AAGCTT	1 cut(s)	1117
HinfI	GANTC	7 cut(s)	805, 1302, 1751, 2171, 2327, 2813, 2944
HpaI	GTTAAC	3 cut(s)	529, 606, 1333
HpaII	CCGG	4 cut(s)	1283, 1669, 2092, 2489
HphI	GGTGA	4 cut(s)	1277, 1297, 1933, 2716
HpyAV	CCTTC	8 cut(s)	920, 1567, 1954, 1994, 2240, 2332, 2624, 2786
HpyCH4III	ACNGT	9 cut(s)	40, 57, 208, 502, 1101, 1205, 1471, 1523, 2537
HpyCH4IV	ACGT	2 cut(s)	826, 2963
HpyF10VI	GCNNNNNNNGC	5 cut(s)	630, 875, 1820, 1924, 2266
HpySE526I	ACGT	2 cut(s)	826, 2963
HspAI	GCGC	1 cut(s)	1407
Kpn2I	TCCGGA	1 cut(s)	2488
KspAI	GTTAAC	3 cut(s)	529, 606, 1333
LmnI	GCTCC	3 cut(s)	227, 1820, 2491
Lsp1109I	GCAGC	5 cut(s)	561, 890, 959, 1433, 1948
LweI	GCATC	8 cut(s)	156, 682, 1258, 1782, 2140, 2745, 2867, 2923
MaeI	CTAG	7 cut(s)	686, 699, 1032, 1565, 2378, 2663, 2991
MaeII	ACGT	2 cut(s)	826, 2963
MaeIII	GTNAC	4 cut(s)	309, 626, 767, 845
MboII	GAAGA	5 cut(s)	668, 1256, 1542, 2711, 2943
MfeI	CAATTG	1 cut(s)	300
MflI	RGATCY	8 cut(s)	274, 690, 904, 1045, 1921, 2400, 2668, 2971
MhlI	GDGCHC	1 cut(s)	2629
MlsI	TGGCCA	1 cut(s)	2420
MluNI	TGGCCA	1 cut(s)	2420
MlyI	GAGTC	3 cut(s)	814, 1745, 2336
MmeI	TCCRAC	4 cut(s)	369, 1786, 1798, 2430
Mox20I	TGGCCA	1 cut(s)	2420
Mph1103I	ATGCAT	3 cut(s)	149, 2291, 2738
MroI	TCCGGA	1 cut(s)	2488
MroXI	GAANNNNTTC	2 cut(s)	1109, 1306
MscI	TGGCCA	1 cut(s)	2420
MslI	CAYNNNNRTG	1 cut(s)	407
Msp20I	TGGCCA	1 cut(s)	2420
MspA1I	CMGCKG	1 cut(s)	354
MspCI	CTTAAG	1 cut(s)	739
MspI	CCGG	4 cut(s)	1283, 1669, 2092, 2489
MspR9I	CCNGG	6 cut(s)	435, 579, 1259, 1451, 2093, 2219
MunI	CAATTG	1 cut(s)	300
Mva1269I	GAATGC	1 cut(s)	1857
MvaI	CCWGG	5 cut(s)	435, 579, 1259, 1451, 2219
MvnI	CGCG	2 cut(s)	367, 2054
MwoI	GCNNNNNNNGC	5 cut(s)	630, 875, 1820, 1924, 2266
NciI	CCSGG	1 cut(s)	2093
NdeI	CATATG	1 cut(s)	1720
NlaIV	GGNNCC	6 cut(s)	426, 1154, 2470, 2487, 2670, 2683
NmuCI	GTSAC	1 cut(s)	767
NsiI	ATGCAT	3 cut(s)	149, 2291, 2738
NspI	RCATGY	3 cut(s)	490, 823, 2788
PaeI	GCATGC	1 cut(s)	2788
PagI	TCATGA	2 cut(s)	508, 2820
PceI	AGGCCT	1 cut(s)	2761
PciI	ACATGT	1 cut(s)	486
PctI	GAATGC	1 cut(s)	1857
PdmI	GAANNNNTTC	2 cut(s)	1109, 1306
PfeI	GAWTC	4 cut(s)	1302, 2171, 2813, 2944
PkrI	GCNGC	5 cut(s)	576, 880, 949, 1448, 1963
PleI	GAGTC	3 cut(s)	813, 1745, 2335
PpsI	GAGTC	3 cut(s)	813, 1745, 2335
PpuMI	RGGWCCY	1 cut(s)	2249
PscI	ACATGT	1 cut(s)	486
PshBI	ATTAAT	1 cut(s)	405
Psp1406I	AACGTT	1 cut(s)	826
Psp5II	RGGWCCY	1 cut(s)	2249
Psp6I	CCWGG	5 cut(s)	433, 577, 1257, 1449, 2217
PspGI	CCWGG	5 cut(s)	433, 577, 1257, 1449, 2217
PspN4I	GGNNCC	6 cut(s)	426, 1154, 2470, 2487, 2670, 2683
PspPI	GGNCC	8 cut(s)	425, 713, 857, 1724, 2237, 2249, 2682, 2987
PspPPI	RGGWCCY	1 cut(s)	2249
PstI	CTGCAG	1 cut(s)	1816
PsuI	RGATCY	8 cut(s)	274, 690, 904, 1045, 1921, 2400, 2668, 2971
RsaI	GTAC	6 cut(s)	358, 1187, 1400, 2044, 2346, 2899
RsaNI	GTAC	6 cut(s)	357, 1186, 1399, 2043, 2345, 2898
RseI	CAYNNNNRTG	1 cut(s)	407
SatI	GCNGC	5 cut(s)	575, 879, 948, 1447, 1962
Sau96I	GGNCC	8 cut(s)	425, 713, 857, 1724, 2237, 2249, 2682, 2987
ScaI	AGTACT	1 cut(s)	1187
SchI	GAGTC	3 cut(s)	814, 1745, 2336
ScrFI	CCNGG	6 cut(s)	435, 579, 1259, 1451, 2093, 2219
SduI	GDGCHC	1 cut(s)	2629
SfaNI	GCATC	8 cut(s)	156, 682, 1258, 1782, 2140, 2745, 2867, 2923
SfcI	CTRYAG	2 cut(s)	1746, 1812
SinI	GGWCC	7 cut(s)	425, 713, 857, 1724, 2249, 2682, 2987
SmiMI	CAYNNNNRTG	1 cut(s)	407
SmlI	CTYRAG	3 cut(s)	739, 802, 2863
SmoI	CTYRAG	3 cut(s)	739, 802, 2863
SpeI	ACTAGT	1 cut(s)	1564
SphI	GCATGC	1 cut(s)	2788
SseBI	AGGCCT	1 cut(s)	2761
SsiI	CCGC	6 cut(s)	354, 365, 867, 2004, 2054, 2124
SspI	AATATT	2 cut(s)	900, 934
SspMI	CTAG	7 cut(s)	686, 699, 1032, 1565, 2378, 2663, 2991
StuI	AGGCCT	1 cut(s)	2761
StyD4I	CCNGG	6 cut(s)	433, 577, 1257, 1449, 2091, 2217
StyI	CCWWGG	2 cut(s)	709, 2672
TaaI	ACNGT	9 cut(s)	40, 57, 208, 502, 1101, 1205, 1471, 1523, 2537
TaiI	ACGT	2 cut(s)	829, 2966
TaqI	TCGA	5 cut(s)	725, 1158, 1977, 2691, 2790
TaqII	GACCGA	2 cut(s)	442, 2806
TatI	WGTACW	4 cut(s)	1185, 1398, 2344, 2897
TfiI	GAWTC	4 cut(s)	1302, 2171, 2813, 2944
TscAI	CASTG	7 cut(s)	211, 643, 772, 971, 1000, 1318, 2340
TseFI	GTSAC	1 cut(s)	767
TseI	GCWGC	5 cut(s)	574, 878, 947, 1446, 1961
Tsp45I	GTSAC	1 cut(s)	767
TspGWI	ACGGA	2 cut(s)	724, 1549
TspRI	CASTG	7 cut(s)	211, 643, 772, 971, 1000, 1318, 2340
Vha464I	CTTAAG	1 cut(s)	739
VpaK11BI	GGWCC	7 cut(s)	425, 713, 857, 1724, 2249, 2682, 2987
VspI	ATTAAT	1 cut(s)	405
XapI	RAATTY	2 cut(s)	791, 1274
XceI	RCATGY	3 cut(s)	490, 823, 2788
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2479
XmiI	GTMKAC	3 cut(s)	1548, 1749, 2019
XmnI	GAANNNNTTC	2 cut(s)	1109, 1306
XspI	CTAG	7 cut(s)	686, 699, 1032, 1565, 2378, 2663, 2991
ZrmI	AGTACT	1 cut(s)	1187
Zsp2I	ATGCAT	3 cut(s)	149, 2291, 2738

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.