Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000134

Physical Location & Seq

Genomic Coordinates Reverse (-)

36431431 .. 36434127

Gene Structure 2697 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1272800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1746 bp

ATGCTGTTTTTCTTCTTTATAGAACAGTATTCGGTGCATATAAATTGTGGTGGAAAACAAGTCACCGTTGGAAGCTTAAAATATGATGCTGATGAAGCTTCAGGAGGCGGAGCAAAGTTTTTTCATCATGAGACAAGGAATTGGGGATTTAGTAGCACTGGTGAATTTGGGATTAAGAATTTTGACCATTACTTCATAGCAGAAAATATCTCCTTACTCAAAATGACCAACTCTGAATTGTACAGAACTGCAAGAATTTCTCCTCTTTCTCTCACATACTATGCCCGTTGCTTAGGAAACGGAAATTACACCGTGAAATTACACTTTGCGGAAATAGTGATGAGAGACAATAGGTCTTATTACAGTGTTGGAAGACGAATTTTTGATGTATATATCCAGGAAAAATTGGTAATGAAAGATTTCAATATTGAAAAGGAAGCACAAGGGGTTGATAAGGAAGTGATTAAGGTATTTAAAGCAGTTGTCAGTGATAGGGCTTTAGAGATCCGATTTTATTGGGCCGGGAAAGGGACATCAAATGCTCCGAGAAGAGGAATATATGGTTCTCTTATATCTGCTATCTCAGTGGAGTCTGATTTCAAACCTCCCGATGATAGAAAAACGAAGATATTCATTGTGGTTGGAGTTCTTTCAGCGCTGTGCCTCTTTTTCATAACTGTTGGCATTCTTTGGCTAACATACTTTAAGGACCGTAGGAGATCTACTAGGGAACAAGATCTGAAAGGATTAGATCTGCAAACTGGTTTCTTTGGGTTCAAACAAATCAAAGCTGCCACTAACAACTTCGACGCTGCAAACAAGCTTGGGGAAGGTGGCTTTGGAGCTGTTTACAAGGGCAAATTACTAGATGGTACTACTATTGCAGTTAAGCAACTATCTTCTACATCACAACAAGGAAACCGTGAATTTGTGAATGAAATAGGCATGATTTCTGCTTTACGACACCCAAATCTCGTAAGATTGTATGGATGTTGCATTGAAGGAAATCAATTATTATTGGTATATGAATATATGGAGAACAATAGCCTTGCACATTCTTTATTTGGTCAAGAGGAAGGCCTATTTAATCTGGACTGGCCTACAAGGCAGAAAATATGCACTGGCATTGCAAGAGGTTTGACTTTTATGCATGAGGAGTCGACACTGAAAATTGTGCATAGAGACATCAAAGCTACAAATATATTGCTTGATGGGGACCTGAACCCTAAAATCTCTGACTTTGGTTTGGCCAAGCTAGACGAAGAGGATAAGACCCGTATTATCACCCGAGTAGCTGGAACTATAGGATACATGGCCCCGGAATATGCATTATGGGGGCATTTAACGGAAAAAGTTGATGTGTACAGTTTTGGGGTTGTTGCACTGGAACTCGTATCTGGAAAAAACAACATAAAGAATTGGCCGAATGAGAGTTATGTATGCCTTCTAGATTGGGCCTGTGTTTTGCAACAGAGGGGAGATCTGATGGAGCTGGTGGATCCAATGTTGGGTTCTGAGTTCAACGAGCAAGAGGCGATGAGAATGATTAAGGTAGCTCTACTATGCACTAATCGATCACCAACACTGAGACCTACAATGTCTGCAGTAGTGAGCATGCTTGAAGGTCGAGCTATAGTTCGTGAACTACTGAACGTAAATTCGAGTATTTACAGTGATGAAGTGAGGTTTAAGTCCTTAAGATATGTGTCTGGGGATTCTGACCCGACTAGTGAAGAGTTTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0002682 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009814 GO:0009861 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0031347 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

582

Amino Acids

65.59

Weight (kDa)

6.82

Isoelectric Point (pI)

35.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin	PF11721	11 - 194	4.4e-38	Malectin domain
Pkinase	PF00069	271 - 537	6.6e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	272 - 538	4.1e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000134)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g06801 FvH4_3g06801 FvH4_3g06810 FvH4_3g06830 FvH4_3g06840 FvH4_3g06840 FvH4_3g06840 FvH4_3g06860 FvH4_3g06860 FvH4_3g06900 FvH4_3g06920 FvH4_3g06940 FvH4_3g06960 FvH4_3g06991 FvH4_3g06991 FvH4_3g07041 FvH4_3g07042 FvH4_3g07044 FvH4_3g07044 FvH4_3g07044 FvH4_3g07044 FvH4_3g20230 FvH4_3g20240 FvH4_3g20240 FvH4_3g20240 FvH4_3g20240 FvH4_4g01431 FvH4_4g01431 FvH4_4g01520
malus_domestica	MD00G1006500.v1.1 MD00G1006600.v1.1 MD00G1186000.v1.1 MD05G1294300.v1.1 MD05G1294500.v1.1 MD05G1294700.v1.1 MD05G1294800.v1.1 MD05G1295100.v1.1 MD10G1272300.v1.1 MD10G1272800.v1.1 MD10G1273000.v1.1 MD10G1273200.v1.1 MD10G1273300.v1.1
prunus_persica	Prupe.4G069300_v2.0.a1 Prupe.4G069400_v2.0.a1 Prupe.4G069400_v2.0.a1 Prupe.4G069500_v2.0.a1 Prupe.4G069600_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069800_v2.0.a1
pyrus_communis	pycom05g27150 pycom10g22770 pycom10g22780 pycom10g22790 pycom10g22800 pycom11g23550 pycom11g23590
rosa_chinensis	RchiOBHm_Chr1g0336311 RchiOBHm_Chr3g0460871 RchiOBHm_Chr5g0012161 RchiOBHm_Chr5g0012181 RchiOBHm_Chr5g0012191 RchiOBHm_Chr5g0012201 RchiOBHm_Chr5g0012221 RchiOBHm_Chr5g0012241 RchiOBHm_Chr5g0012251 RchiOBHm_Chr5g0012261 RchiOBHm_Chr5g0012271 RchiOBHm_Chr5g0012291 RchiOBHm_Chr5g0012301 RchiOBHm_Chr5g0012321 RchiOBHm_Chr5g0034051 RchiOBHm_Chr5g0034071 RchiOBHm_Chr5g0034091 RchiOBHm_Chr5g0080321 RchiOBHm_Chr7g0226661
rosa_laevigata	RLG00000001709 RLG00000003039 RLG00000009208 RLG00000021256 RLG00000031886 RLG00000031891 RLG00000031892 RLG00000031895 RLG00000031896 RLG00000031897 RLG00000031898 RLG00000031899 RLG00000031900 RLG00000031903 RLG00000033520 RLG00000033522 RLG00000033524 RLG00000033525
rosa_multiflora	Rmu_co8172290.1_g000001 Rmu_co8214942.1_g000001 Rmu_co8328697.1_g000001 Rmu_sc0001608.1_g000041 Rmu_sc0003368.1_g000024 Rmu_sc0003701.1_g000002 Rmu_sc0003765.1_g000052 Rmu_sc0005343.1_g000007 Rmu_sc0005343.1_g000008 Rmu_sc0006603.1_g000004 Rmu_sc0007030.1_g000001 Rmu_sc0007030.1_g000006 Rmu_sc0007176.1_g000013 Rmu_sc0009761.1_g000006 Rmu_sc0009761.1_g000007 Rmu_sc0012149.1_g000012 Rmu_sc0013327.1_g000001 Rmu_sc0013327.1_g000003 Rmu_sc0014215.1_g000004 Rmu_ssc0000128.1_g000005 Rmu_ssc0000128.1_g000012 Rmu_ssc0000128.1_g000016 Rmu_ssc0000217.1_g000012 Rmu_ssc0000217.1_g000014 Rmu_ssc0000217.1_g000018 Rmu_ssc0000217.1_g000020 Rmu_ssc0000217.1_g000026 Rmu_ssc0000217.1_g000037
rosa_roxburghii	Rroxscaffold_1G00014960 Rroxscaffold_1G00046140 Rroxscaffold_1G00046190 Rroxscaffold_1G00064330 Rroxscaffold_1G00064370 Rroxscaffold_1G00064380 Rroxscaffold_1G00064390 Rroxscaffold_1G00064400 Rroxscaffold_1G00064420 Rroxscaffold_1G00064430 Rroxscaffold_1G00064460 Rroxscaffold_1G00064470 Rroxscaffold_1G00064480 Rroxscaffold_2G00092560 Rroxscaffold_3G00232520 Rroxscaffold_3G00236850 Rroxscaffold_4G00292720 Rroxscaffold_4G00315770 Rroxscaffold_4G00315780 Rroxscaffold_4G00315790
rosa_rugosa	Rorug01G0123800 Rorug01G0123900 Rorug01G0124000 Rorug01G0124000 Rorug03G0284000 Rorug04G0090200 Rorug05G0000200 Rorug05G0000200 Rorug05G0000300 Rorug05G0000300 Rorug05G0000300 Rorug05G0000300 Rorug05G0000400 Rorug05G0000600 Rorug05G0144300 Rorug05G0144300
rosa_samantha	Rh1CG135900 Rh2DG334300 Rh2DG334500 Rh5AG090300 Rh5AG093400 Rh5AG093500 Rh5AG093900 Rh5AG094000 Rh5AG094100 Rh5AG094200 Rh5AG094400 Rh5AG094600 Rh5AG100500 Rh5AG235400 Rh5AG235500 Rh5BG089400 Rh5BG412100 Rh5CG103100 Rh5CG103800 Rh5CG103900 Rh6AG058500 Rh6BG478600 Rh6CG484600 Rh7AG387000
rosa_wichuraiana	Rw0G004700 Rw1G011820 Rw5G007880 Rw5G008160 Rw5G008170 Rw5G008180 Rw5G008200 Rw5G008210 Rw5G008220 Rw5G008230 Rw5G008240 Rw5G008260 Rw5G021250 Rw5G021260 Rw5G021390 Rw5G021400 Rw5G021410 Rw5G021420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1160
AciI	CCGC	2 cut(s)	108, 329
AclWI	GGATC	3 cut(s)	499, 1493, 1506
AcoI	YGGCCR	2 cut(s)	1248, 1421
AcsI	RAATTY	6 cut(s)	164, 178, 255, 378, 926, 1657
AcuI	CTGAAG	1 cut(s)	84
AfaI	GTAC	3 cut(s)	242, 874, 1364
AfeI	AGCGCT	1 cut(s)	657
AfiI	CCNNNNNNNGG	3 cut(s)	528, 551, 1508
AflII	CTTAAG	1 cut(s)	1696
AgsI	TTSAA	7 cut(s)	424, 431, 601, 778, 1001, 1522, 1622
AhdI	GACNNNNNGTC	1 cut(s)	352
AhlI	ACTAGT	1 cut(s)	1727
AjnI	CCWGG	1 cut(s)	396
AjuI	GAANNNNNNNTTGG	4 cut(s)	822, 854, 1495, 1527
Alw26I	GTCTC	4 cut(s)	125, 339, 1176, 1582
AlwI	GGATC	3 cut(s)	499, 1493, 1506
Ama87I	CYCGRG	1 cut(s)	1287
Aor51HI	AGCGCT	1 cut(s)	657
AoxI	GGCC	7 cut(s)	519, 1078, 1097, 1248, 1314, 1421, 1455
ApeKI	GCWGC	2 cut(s)	791, 812
ApoI	RAATTY	6 cut(s)	164, 178, 255, 378, 926, 1657
Asp700I	GAANNNNTTC	2 cut(s)	419, 629
AspLEI	GCGC	1 cut(s)	658
AspS9I	GGNCC	5 cut(s)	519, 709, 1216, 1315, 1455
AsuC2I	CCSGG	2 cut(s)	523, 1319
AsuHPI	GGTGA	4 cut(s)	55, 173, 1276, 1569
AvaI	CYCGRG	1 cut(s)	1287
AvaII	GGWCC	2 cut(s)	709, 1216
BalI	TGGCCA	1 cut(s)	1250
BamHI	GGATCC	1 cut(s)	1498
BbsI	GAAGAC	1 cut(s)	379
BbvI	GCAGC	2 cut(s)	778, 799
BccI	CCATC	3 cut(s)	863, 1205, 1480
BciT130I	CCWGG	1 cut(s)	398
BciVI	GTATCC	1 cut(s)	1301
BcnI	CCSGG	2 cut(s)	523, 1319
BcoDI	GTCTC	4 cut(s)	125, 339, 1176, 1582
BcuI	ACTAGT	1 cut(s)	1727
BfaI	CTAG	5 cut(s)	726, 866, 1256, 1448, 1728
BfmI	CTRYAG	3 cut(s)	1302, 1602, 1632
BfoI	RGCGCY	1 cut(s)	659
BfrI	CTTAAG	1 cut(s)	1696
BfuI	GTATCC	1 cut(s)	1301
BglI	GCCNNNNNGGC	1 cut(s)	1105
BglII	AGATCT	4 cut(s)	719, 736, 751, 1480
BisI	GCNGC	2 cut(s)	792, 813
BlsI	GCNGC	2 cut(s)	793, 814
Bme1390I	CCNGG	3 cut(s)	398, 523, 1319
Bme18I	GGWCC	2 cut(s)	709, 1216
BmeRI	GACNNNNNGTC	1 cut(s)	352
BmeT110I	CYCGRG	1 cut(s)	1287
BmgT120I	GGNCC	5 cut(s)	519, 709, 1216, 1315, 1455
BmiI	GGNNCC	3 cut(s)	1217, 1317, 1500
BmrFI	CCNGG	3 cut(s)	398, 523, 1319
BmsI	GCATC	1 cut(s)	76
BpiI	GAAGAC	1 cut(s)	379
Bpu10I	CCTNAGC	1 cut(s)	292
BpuMI	CCSGG	2 cut(s)	523, 1319
Bsa29I	ATCGAT	1 cut(s)	1573
BsaI	GGTCTC	1 cut(s)	1582
BsaJI	CCNNGG	1 cut(s)	1317
Bsc4I	CCNNNNNNNGG	3 cut(s)	528, 551, 1508
Bse1I	ACTGG	5 cut(s)	163, 766, 1100, 1126, 1389
Bse3DI	GCAATG	1 cut(s)	1125
BseBI	CCWGG	1 cut(s)	398
BseCI	ATCGAT	1 cut(s)	1573
BseDI	CCNNGG	1 cut(s)	1317
BseGI	GGATG	1 cut(s)	995
BseLI	CCNNNNNNNGG	3 cut(s)	528, 551, 1508
BseMI	GCAATG	1 cut(s)	1125
BseMII	CTCAG	3 cut(s)	597, 1506, 1577
BseNI	ACTGG	5 cut(s)	163, 766, 1100, 1126, 1389
BseRI	GAGGAG	2 cut(s)	252, 1169
BseXI	GCAGC	2 cut(s)	778, 799
BshFI	GGCC	7 cut(s)	521, 1080, 1099, 1250, 1316, 1423, 1457
BshVI	ATCGAT	1 cut(s)	1573
BsiHKCI	CYCGRG	1 cut(s)	1287
BsiSI	CCGG	2 cut(s)	522, 1319
BslFI	GGGAC	2 cut(s)	544, 1229
BslI	CCNNNNNNNGG	3 cut(s)	528, 551, 1508
BsmAI	GTCTC	4 cut(s)	125, 339, 1176, 1582
BsmFI	GGGAC	2 cut(s)	544, 1229
BsmI	GAATGC	1 cut(s)	684
BsnI	GGCC	7 cut(s)	521, 1080, 1099, 1250, 1316, 1423, 1457
Bso31I	GGTCTC	1 cut(s)	1582
BsoBI	CYCGRG	1 cut(s)	1287
Bsp1407I	TGTACA	2 cut(s)	240, 1362
Bsp143I	GATC	7 cut(s)	504, 719, 736, 751, 1480, 1498, 1574
BspACI	CCGC	2 cut(s)	108, 329
BspANI	GGCC	7 cut(s)	521, 1080, 1099, 1250, 1316, 1423, 1457
BspCNI	CTCAG	3 cut(s)	596, 1507, 1578
BspDI	ATCGAT	1 cut(s)	1573
BspHI	TCATGA	1 cut(s)	127
BspLI	GGNNCC	3 cut(s)	1217, 1317, 1500
BspMAI	CTGCAG	1 cut(s)	1606
BspPI	GGATC	3 cut(s)	499, 1493, 1506
BspTI	CTTAAG	1 cut(s)	1696
BspTNI	GGTCTC	1 cut(s)	1582
BsrDI	GCAATG	1 cut(s)	1125
BsrGI	TGTACA	2 cut(s)	240, 1362
BsrI	ACTGG	5 cut(s)	163, 766, 1100, 1126, 1389
BssECI	CCNNGG	1 cut(s)	1317
BssMI	GATC	7 cut(s)	504, 719, 736, 751, 1480, 1498, 1574
Bst2UI	CCWGG	1 cut(s)	398
Bst4CI	ACNGT	9 cut(s)	27, 67, 313, 365, 679, 713, 923, 1367, 1673
Bst6I	CTCTTC	3 cut(s)	544, 1257, 1728
BstAFI	CTTAAG	1 cut(s)	1696
BstAUI	TGTACA	2 cut(s)	240, 1362
BstC8I	GCNNGC	1 cut(s)	1616
BstDEI	CTNAG	4 cut(s)	292, 583, 1515, 1586
BstF5I	GGATG	1 cut(s)	995
BstH2I	RGCGCY	1 cut(s)	659
BstHHI	GCGC	1 cut(s)	658
BstKTI	GATC	7 cut(s)	507, 722, 739, 754, 1483, 1501, 1577
BstMAI	GTCTC	4 cut(s)	125, 339, 1176, 1582
BstMBI	GATC	7 cut(s)	504, 719, 736, 751, 1480, 1498, 1574
BstMWI	GCNNNNNNNGC	2 cut(s)	95, 1105
BstNI	CCWGG	1 cut(s)	398
BstNSI	RCATGY	1 cut(s)	1618
BstSCI	CCNGG	3 cut(s)	396, 521, 1317
BstSFI	CTRYAG	3 cut(s)	1302, 1602, 1632
BstV1I	GCAGC	2 cut(s)	778, 799
BstV2I	GAAGAC	1 cut(s)	379
BstX2I	RGATCY	6 cut(s)	504, 719, 736, 751, 1480, 1498
BstYI	RGATCY	6 cut(s)	504, 719, 736, 751, 1480, 1498
Bsu15I	ATCGAT	1 cut(s)	1573
BsuI	GTATCC	1 cut(s)	1301
BsuRI	GGCC	7 cut(s)	521, 1080, 1099, 1250, 1316, 1423, 1457
BsuTUI	ATCGAT	1 cut(s)	1573
BtgZI	GCGATG	1 cut(s)	1550
BtsCI	GGATG	1 cut(s)	995
BtsIMutI	CAGTG	9 cut(s)	156, 370, 493, 591, 1119, 1163, 1382, 1583, 1678
Cac8I	GCNNGC	1 cut(s)	1616
CciI	TCATGA	1 cut(s)	127
CfoI	GCGC	1 cut(s)	658
Cfr13I	GGNCC	5 cut(s)	519, 709, 1216, 1315, 1455
ClaI	ATCGAT	1 cut(s)	1573
CseI	GACGC	1 cut(s)	818
Csp6I	GTAC	3 cut(s)	241, 873, 1363
CviAII	CATG	5 cut(s)	128, 946, 1151, 1312, 1615
CviQI	GTAC	3 cut(s)	241, 873, 1363
DdeI	CTNAG	4 cut(s)	292, 583, 1515, 1586
DpnI	GATC	7 cut(s)	506, 721, 738, 753, 1482, 1500, 1576
DpnII	GATC	7 cut(s)	504, 719, 736, 751, 1480, 1498, 1574
DraI	TTTAAA	1 cut(s)	475
DriI	GACNNNNNGTC	1 cut(s)	352
EaeI	YGGCCR	2 cut(s)	1248, 1421
Eam1104I	CTCTTC	3 cut(s)	544, 1257, 1728
Eam1105I	GACNNNNNGTC	1 cut(s)	352
EarI	CTCTTC	3 cut(s)	544, 1257, 1728
EciI	GGCGGA	1 cut(s)	123
Eco147I	AGGCCT	1 cut(s)	1080
Eco31I	GGTCTC	1 cut(s)	1582
Eco47I	GGWCC	2 cut(s)	709, 1216
Eco47III	AGCGCT	1 cut(s)	657
Eco57I	CTGAAG	1 cut(s)	84
Eco88I	CYCGRG	1 cut(s)	1287
EcoO109I	RGGNCCY	1 cut(s)	1216
EcoRII	CCWGG	1 cut(s)	396
EcoT22I	ATGCAT	2 cut(s)	1152, 1330
FaeI	CATG	5 cut(s)	131, 949, 1154, 1315, 1618
FaqI	GGGAC	2 cut(s)	544, 1229
FatI	CATG	5 cut(s)	127, 945, 1150, 1311, 1614
FblI	GTMKAC	1 cut(s)	1160
Fnu4HI	GCNGC	2 cut(s)	792, 813
FokI	GGATG	1 cut(s)	1002
Fsp4HI	GCNGC	2 cut(s)	792, 813
FspBI	CTAG	5 cut(s)	726, 866, 1256, 1448, 1728
GlaI	GCGC	1 cut(s)	657
GluI	GCNGC	2 cut(s)	792, 813
HaeII	RGCGCY	1 cut(s)	659
HaeIII	GGCC	7 cut(s)	521, 1080, 1099, 1250, 1316, 1423, 1457
HapII	CCGG	2 cut(s)	522, 1319
HgaI	GACGC	1 cut(s)	818
HhaI	GCGC	1 cut(s)	658
Hin1II	CATG	5 cut(s)	131, 949, 1154, 1315, 1618
Hin6I	GCGC	1 cut(s)	656
HinP1I	GCGC	1 cut(s)	656
HincII	GTYRAC	1 cut(s)	1161
HindII	GTYRAC	1 cut(s)	1161
HindIII	AAGCTT	3 cut(s)	73, 96, 821
HinfI	GANTC	3 cut(s)	590, 1157, 1715
HpaII	CCGG	2 cut(s)	522, 1319
HphI	GGTGA	4 cut(s)	55, 173, 1276, 1569
Hpy166II	GTNNAC	4 cut(s)	850, 1161, 1363, 1643
Hpy188I	TCNGA	9 cut(s)	235, 509, 546, 595, 741, 1237, 1485, 1516, 1720
Hpy188III	TCNNGA	8 cut(s)	102, 128, 608, 1070, 1091, 1398, 1448, 1640
Hpy8I	GTNNAC	4 cut(s)	850, 1161, 1363, 1643
Hpy99I	CGWCG	1 cut(s)	812
HpyAV	CCTTC	5 cut(s)	824, 995, 1070, 1454, 1616
HpyCH4III	ACNGT	9 cut(s)	27, 67, 313, 365, 679, 713, 923, 1367, 1673
HpyCH4IV	ACGT	1 cut(s)	1653
HpyF10VI	GCNNNNNNNGC	2 cut(s)	95, 1105
HpyF3I	CTNAG	4 cut(s)	292, 583, 1515, 1586
HpySE526I	ACGT	1 cut(s)	1653
Hsp92II	CATG	5 cut(s)	131, 949, 1154, 1315, 1618
HspAI	GCGC	1 cut(s)	656
Kzo9I	GATC	7 cut(s)	504, 719, 736, 751, 1480, 1498, 1574
LmnI	GCTCC	4 cut(s)	110, 547, 842, 1489
Lsp1109I	GCAGC	2 cut(s)	778, 799
LweI	GCATC	1 cut(s)	76
MaeI	CTAG	5 cut(s)	726, 866, 1256, 1448, 1728
MaeII	ACGT	1 cut(s)	1653
MaeIII	GTNAC	1 cut(s)	61
MalI	GATC	7 cut(s)	506, 721, 738, 753, 1482, 1500, 1576
MboI	GATC	7 cut(s)	504, 719, 736, 751, 1480, 1498, 1574
MboII	GAAGA	7 cut(s)	4, 384, 561, 637, 891, 1274, 1745
MflI	RGATCY	6 cut(s)	504, 719, 736, 751, 1480, 1498
MlsI	TGGCCA	1 cut(s)	1250
MluNI	TGGCCA	1 cut(s)	1250
MlyI	GAGTC	2 cut(s)	599, 1166
MmeI	TCCRAC	3 cut(s)	49, 349, 622
Mox20I	TGGCCA	1 cut(s)	1250
Mph1103I	ATGCAT	2 cut(s)	1152, 1330
MroXI	GAANNNNTTC	2 cut(s)	419, 629
MscI	TGGCCA	1 cut(s)	1250
Msp20I	TGGCCA	1 cut(s)	1250
MspCI	CTTAAG	1 cut(s)	1696
MspI	CCGG	2 cut(s)	522, 1319
MspR9I	CCNGG	3 cut(s)	398, 523, 1319
Mva1269I	GAATGC	1 cut(s)	684
MvaI	CCWGG	1 cut(s)	398
MwoI	GCNNNNNNNGC	2 cut(s)	95, 1105
NciI	CCSGG	2 cut(s)	523, 1319
NdeII	GATC	7 cut(s)	504, 719, 736, 751, 1480, 1498, 1574
NlaIII	CATG	5 cut(s)	131, 949, 1154, 1315, 1618
NlaIV	GGNNCC	3 cut(s)	1217, 1317, 1500
NmuCI	GTSAC	1 cut(s)	61
NsiI	ATGCAT	2 cut(s)	1152, 1330
NspI	RCATGY	1 cut(s)	1618
PaeI	GCATGC	1 cut(s)	1618
PagI	TCATGA	1 cut(s)	127
PceI	AGGCCT	1 cut(s)	1080
PctI	GAATGC	1 cut(s)	684
PdmI	GAANNNNTTC	2 cut(s)	419, 629
PfeI	GAWTC	1 cut(s)	1715
PfoI	TCCNGGA	1 cut(s)	396
PkrI	GCNGC	2 cut(s)	793, 814
PleI	GAGTC	2 cut(s)	598, 1165
PpsI	GAGTC	2 cut(s)	598, 1165
PpuMI	RGGWCCY	1 cut(s)	1216
Psp5II	RGGWCCY	1 cut(s)	1216
Psp6I	CCWGG	1 cut(s)	396
PspGI	CCWGG	1 cut(s)	396
PspN4I	GGNNCC	3 cut(s)	1217, 1317, 1500
PspPI	GGNCC	5 cut(s)	519, 709, 1216, 1315, 1455
PspPPI	RGGWCCY	1 cut(s)	1216
PstI	CTGCAG	1 cut(s)	1606
PsuI	RGATCY	6 cut(s)	504, 719, 736, 751, 1480, 1498
RsaI	GTAC	3 cut(s)	242, 874, 1364
RsaNI	GTAC	3 cut(s)	241, 873, 1363
SalI	GTCGAC	1 cut(s)	1159
SatI	GCNGC	2 cut(s)	792, 813
Sau3AI	GATC	7 cut(s)	504, 719, 736, 751, 1480, 1498, 1574
Sau96I	GGNCC	5 cut(s)	519, 709, 1216, 1315, 1455
SchI	GAGTC	2 cut(s)	599, 1166
ScrFI	CCNGG	3 cut(s)	398, 523, 1319
SfaNI	GCATC	1 cut(s)	76
SfcI	CTRYAG	3 cut(s)	1302, 1602, 1632
SinI	GGWCC	2 cut(s)	709, 1216
SmlI	CTYRAG	1 cut(s)	1696
SmoI	CTYRAG	1 cut(s)	1696
SpeI	ACTAGT	1 cut(s)	1727
SphI	GCATGC	1 cut(s)	1618
SseBI	AGGCCT	1 cut(s)	1080
SsiI	CCGC	2 cut(s)	108, 329
SspI	AATATT	1 cut(s)	427
SspMI	CTAG	5 cut(s)	726, 866, 1256, 1448, 1728
StuI	AGGCCT	1 cut(s)	1080
StyD4I	CCNGG	3 cut(s)	396, 521, 1317
TaaI	ACNGT	9 cut(s)	27, 67, 313, 365, 679, 713, 923, 1367, 1673
TaiI	ACGT	1 cut(s)	1656
TaqI	TCGA	5 cut(s)	807, 1160, 1573, 1627, 1661
TatI	WGTACW	2 cut(s)	240, 1362
TfiI	GAWTC	1 cut(s)	1715
TscAI	CASTG	9 cut(s)	163, 370, 493, 591, 1126, 1170, 1389, 1590, 1678
TseFI	GTSAC	1 cut(s)	61
TseI	GCWGC	2 cut(s)	791, 812
Tsp45I	GTSAC	1 cut(s)	61
TspDTI	ATGAA	9 cut(s)	108, 113, 184, 428, 622, 661, 951, 1041, 1692
TspGWI	ACGGA	2 cut(s)	315, 1361
TspRI	CASTG	9 cut(s)	163, 370, 493, 591, 1126, 1170, 1389, 1590, 1678
Vha464I	CTTAAG	1 cut(s)	1696
VpaK11BI	GGWCC	2 cut(s)	709, 1216
XapI	RAATTY	6 cut(s)	164, 178, 255, 378, 926, 1657
XbaI	TCTAGA	1 cut(s)	1447
XceI	RCATGY	1 cut(s)	1618
XmiI	GTMKAC	1 cut(s)	1160
XmnI	GAANNNNTTC	2 cut(s)	419, 629
XspI	CTAG	5 cut(s)	726, 866, 1256, 1448, 1728
Zsp2I	ATGCAT	2 cut(s)	1152, 1330

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.