Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000134

Physical Location & Seq

Genomic Coordinates Forward (+)

49960462 .. 49966372

Gene Structure 5911 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ20301

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2436 bp

ATGCAGAATTACCTTAGTGGTTCAATTCCACCCGAATGGGGTTCTACCAAGCTGGAATTTCTGGTTCTCAGTGTGAACAACTTATCAGGACCTATCCCGGCCTATTTGGGGAACATAACTACTCTCCGAGCTTTGGCCCTGGAAAGCAACTTATTTTCTGGAACTATTCCGCCGGAGATGGGGAGATTGATTAACATGGAGGTCCTCTACCTTCGAGCTAACAATCTCACAGGACAGTTGCCTGTGGCTCTCACTAATATGACCAAATTAAAAACACTTTGGATTGGCAGTAACAACTTCACTGGAAAAATACCAAACTATTTTCTTAGTTGGAAAGAACTTCAGACGTTAGAGATGCAAGCAAGTGGCTTTGAAGGACCCATTCCTTCTACGCTTTCTGTCTTAAGTAATATGAAATACCTAGTAATCAGTGACTTAAGTGGTGAGAGCTCAGTTTTTCCAAACTTGTCGAACATGAAAAACATGCAGACATTGATGTTAAGGAGCTGCAATATAACAGGATCAATTCCAGAATATATCTCTAACATGACAAGCCTGCAAGTTTTAGATCTTAGCTTCAACAGATTGAAAGGGAGCATCCCGAATCTTGACAAGTTATTAGGATTGGTCACAATATATCTGACGAGTAACTGGCTTACTGGGCTTCCAGAGTGGATCCAAAATAGAGATAGCCGCTACATAGTAGATCTTTCTTACAACAATTTTTCAAGGGACTCTGTCCCAACTACATGTCGAGAAACCTTTAATGTGTTCAAAAGTTTCTCCAGACAGAACAACTCAATTCTTTCCAATTGCCTCTATCCATGTTCAAAAGAACAGTATTCATTGCATATAAATTGTGGTGGAAAACCAATAACCATTGGACGCATCAAGTATGAGGGGGATGAAGCCTCGGGAGGTGGAGCAAAGTTTTTTCAAGACACAGTTGTTGGGGATTTAGTAGCACTGGTGATTATGGGCTTACAAATTGACCATGCCTACATAGCAAACAATATTTCCATAGTCAAAATGAACAACTCGGAATTGTACAGAACTGCCCGACTTTCTTATCTTTCTCTCACATATTATGCCCGTTGCTTAGCAAATGGAAATTACACTGTGAAACTACACTTTGCAGAAATAGTTCTCAGAGACAACAGGTCTTATTATGGTGTTGGAAGCCGATTTTTTGATGTCTATATTCAGGAAAAACTGGTATTGAAGGATTTTAATATTGAAAAGGCAGCACAGGGGGTTGATAAGGAAGTGATTAAAGTATTTAAAGCAGTTGTCAATGTTAGCACTTTAGAGATCCGTTTTTATTGGGCTGGGAAAGGGACTATGAATGTCCCAAAAAGAGGGACATATGGTTCTCTTATATCAGCTATCTCAATTGAGTCTGATTTCAACCCGCCTGGTGATAGCAACAGGAAAATATTCATAGTGGTTGGAGTTGCTATAGTCTTATGCCTTAGCTTCATGGTTTTTGGCATTCTTTGGTTTAGAAGCTGCTTTGGAGGCAAGATCTCTAGGGAACAAGAGCTGAAAGGATTAGATCTGCAAACTGGTCTCTTTAGATTCAAACAAATCAAAGCTGCCACTAACAACTTTGATGCTGCAAACAAGCTTGGTGAAGGTGGCTTTGGAACTGTTTACAAGTTCTGTCCACAAGAAGGTAAACTGAAACTGGACTGGCCTAAAAGGCAAAAAATATGTATTGGCATTGCAAGAGGGCTAGCTTTCCTGCATGAGGAATCTACACTAAAAATTGTTCATAGAGACATCAAAATGACTAACATACTGCTAGATAGGGACCTTAACCCTAAAATCTCTGACTTTGGTTTGGCCAAGCTGAACGAAGAAGAGAAGACGCATATTAGCACCAGAGTAGCTGGAACCATAGGATACATGGCGCCAGAATATGCACAATGGGGTTACCTAACTGATAAAGTAGACGTTTACAGTTTTGGAGTTGTTGCATTAGAGCTCGTATCTGGCAAAAACAACATCAAATATCGGCCCAATGAGAACTGTGTATGTCTTCTAGATTGGGCCCTTGTTCTGCAACAAAAAGAAAATATGATGGAGCTAGTGGATCCAAATTTGGGATCCGAGTTCAACAAGGAAGAAGCAATGAGAATGATCAAAGTAGCACTGCTCTGCACTAATCCATCTCCAGCACTGAGACCTACAATGTCTGCAGCATTAAGCATGCTTGAAGGCCGAGCCGTTATTCATGAATCAAGTATGAGTTCAAGTATGTATGGTGATGAACTGGGCTTCAAGGCCTTTAGAGATGAGCATCAGGAAGTGAATATTCAACAGAGCCCAGGCGAGACTCGGAGCCTCATTTATTCATCCAATGCCAAAGGGCAAGATGCCTCTTCCTCAAGGACTGTCCAGGATAGCTATACATTTAATCTTGATTCTCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0002682 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009814 GO:0009861 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0031347 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

811

Amino Acids

90.63

Weight (kDa)

8.45

Isoelectric Point (pI)

35.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	34 - 228	3e-10	Leucine-rich repeat region
LRR_4	PF12799	160 - 203	5.9e-06	Leucine Rich repeats (2 copies)
Malectin	PF11721	282 - 463	4.6e-38	Malectin domain
PK_Tyr_Ser-Thr	PF07714	560 - 735	4e-24	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	568 - 737	6.7e-27	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000134)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g06801 FvH4_3g06801 FvH4_3g06810 FvH4_3g06830 FvH4_3g06840 FvH4_3g06840 FvH4_3g06840 FvH4_3g06860 FvH4_3g06860 FvH4_3g06900 FvH4_3g06920 FvH4_3g06940 FvH4_3g06960 FvH4_3g06991 FvH4_3g06991 FvH4_3g07041 FvH4_3g07042 FvH4_3g07044 FvH4_3g07044 FvH4_3g07044 FvH4_3g07044 FvH4_3g20230 FvH4_3g20240 FvH4_3g20240 FvH4_3g20240 FvH4_3g20240 FvH4_4g01431 FvH4_4g01431 FvH4_4g01520
malus_domestica	MD00G1006500.v1.1 MD00G1006600.v1.1 MD00G1186000.v1.1 MD05G1294300.v1.1 MD05G1294500.v1.1 MD05G1294700.v1.1 MD05G1294800.v1.1 MD05G1295100.v1.1 MD10G1272300.v1.1 MD10G1272800.v1.1 MD10G1273000.v1.1 MD10G1273200.v1.1 MD10G1273300.v1.1
prunus_persica	Prupe.4G069300_v2.0.a1 Prupe.4G069400_v2.0.a1 Prupe.4G069400_v2.0.a1 Prupe.4G069500_v2.0.a1 Prupe.4G069600_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069700_v2.0.a1 Prupe.4G069800_v2.0.a1
pyrus_communis	pycom05g27150 pycom10g22770 pycom10g22780 pycom10g22790 pycom10g22800 pycom11g23550 pycom11g23590
rosa_chinensis	RchiOBHm_Chr1g0336311 RchiOBHm_Chr3g0460871 RchiOBHm_Chr5g0012161 RchiOBHm_Chr5g0012181 RchiOBHm_Chr5g0012191 RchiOBHm_Chr5g0012201 RchiOBHm_Chr5g0012221 RchiOBHm_Chr5g0012241 RchiOBHm_Chr5g0012251 RchiOBHm_Chr5g0012261 RchiOBHm_Chr5g0012271 RchiOBHm_Chr5g0012291 RchiOBHm_Chr5g0012301 RchiOBHm_Chr5g0012321 RchiOBHm_Chr5g0034051 RchiOBHm_Chr5g0034071 RchiOBHm_Chr5g0034091 RchiOBHm_Chr5g0080321 RchiOBHm_Chr7g0226661
rosa_laevigata	RLG00000001709 RLG00000003039 RLG00000009208 RLG00000021256 RLG00000031886 RLG00000031891 RLG00000031892 RLG00000031895 RLG00000031896 RLG00000031897 RLG00000031898 RLG00000031899 RLG00000031900 RLG00000031903 RLG00000033520 RLG00000033522 RLG00000033524 RLG00000033525
rosa_multiflora	Rmu_co8172290.1_g000001 Rmu_co8214942.1_g000001 Rmu_co8328697.1_g000001 Rmu_sc0001608.1_g000041 Rmu_sc0003368.1_g000024 Rmu_sc0003701.1_g000002 Rmu_sc0003765.1_g000052 Rmu_sc0005343.1_g000007 Rmu_sc0005343.1_g000008 Rmu_sc0006603.1_g000004 Rmu_sc0007030.1_g000001 Rmu_sc0007030.1_g000006 Rmu_sc0007176.1_g000013 Rmu_sc0009761.1_g000006 Rmu_sc0009761.1_g000007 Rmu_sc0012149.1_g000012 Rmu_sc0013327.1_g000001 Rmu_sc0013327.1_g000003 Rmu_sc0014215.1_g000004 Rmu_ssc0000128.1_g000005 Rmu_ssc0000128.1_g000012 Rmu_ssc0000128.1_g000016 Rmu_ssc0000217.1_g000012 Rmu_ssc0000217.1_g000014 Rmu_ssc0000217.1_g000018 Rmu_ssc0000217.1_g000020 Rmu_ssc0000217.1_g000026 Rmu_ssc0000217.1_g000037
rosa_roxburghii	Rroxscaffold_1G00014960 Rroxscaffold_1G00046140 Rroxscaffold_1G00046190 Rroxscaffold_1G00064330 Rroxscaffold_1G00064370 Rroxscaffold_1G00064380 Rroxscaffold_1G00064390 Rroxscaffold_1G00064400 Rroxscaffold_1G00064420 Rroxscaffold_1G00064430 Rroxscaffold_1G00064460 Rroxscaffold_1G00064470 Rroxscaffold_1G00064480 Rroxscaffold_2G00092560 Rroxscaffold_3G00232520 Rroxscaffold_3G00236850 Rroxscaffold_4G00292720 Rroxscaffold_4G00315770 Rroxscaffold_4G00315780 Rroxscaffold_4G00315790
rosa_rugosa	Rorug01G0123800 Rorug01G0123900 Rorug01G0124000 Rorug01G0124000 Rorug03G0284000 Rorug04G0090200 Rorug05G0000200 Rorug05G0000200 Rorug05G0000300 Rorug05G0000300 Rorug05G0000300 Rorug05G0000300 Rorug05G0000400 Rorug05G0000600 Rorug05G0144300 Rorug05G0144300
rosa_samantha	Rh1CG135900 Rh2DG334300 Rh2DG334500 Rh5AG090300 Rh5AG093400 Rh5AG093500 Rh5AG093900 Rh5AG094000 Rh5AG094100 Rh5AG094200 Rh5AG094400 Rh5AG094600 Rh5AG100500 Rh5AG235400 Rh5AG235500 Rh5BG089400 Rh5BG412100 Rh5CG103100 Rh5CG103800 Rh5CG103900 Rh6AG058500 Rh6BG478600 Rh6CG484600 Rh7AG387000
rosa_wichuraiana	Rw0G004700 Rw1G011820 Rw5G007880 Rw5G008160 Rw5G008170 Rw5G008180 Rw5G008200 Rw5G008210 Rw5G008220 Rw5G008230 Rw5G008240 Rw5G008260 Rw5G021250 Rw5G021260 Rw5G021390 Rw5G021400 Rw5G021410 Rw5G021420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1912
AccI	GTMKAC	1 cut(s)	1953
AciI	CCGC	3 cut(s)	170, 694, 1412
AclWI	GGATC	8 cut(s)	529, 670, 683, 1306, 2090, 2103, 2103, 2116
AcoI	YGGCCR	1 cut(s)	1845
AcsI	RAATTY	2 cut(s)	56, 2101
AcuI	CTGAAG	1 cut(s)	326
AcyI	GRCGYC	1 cut(s)	1913
AfaI	GTAC	1 cut(s)	1049
AfiI	CCNNNNNNNGG	8 cut(s)	38, 108, 133, 179, 1358, 1673, 1750, 2105
AflII	CTTAAG	2 cut(s)	403, 436
AflIII	ACRYGT	1 cut(s)	749
AhdI	GACNNNNNGTC	1 cut(s)	1159
AjnI	CCWGG	4 cut(s)	138, 1414, 2329, 2400
AjuI	GAANNNNNNNTTGG	2 cut(s)	1626, 1658
AloI	GAACNNNNNNTCC	2 cut(s)	1354, 1386
Alw21I	GWGCWC	2 cut(s)	452, 1989
Alw26I	GTCTC	5 cut(s)	1146, 1574, 1773, 2179, 2330
AlwI	GGATC	8 cut(s)	529, 670, 683, 1306, 2090, 2103, 2103, 2116
Ama87I	CYCGRG	1 cut(s)	913
AoxI	GGCC	8 cut(s)	99, 135, 1694, 1845, 2018, 2052, 2221, 2286
ApaI	GGGCCC	1 cut(s)	2056
ApeKI	GCWGC	6 cut(s)	507, 1244, 1509, 1595, 1616, 2201
ApoI	RAATTY	2 cut(s)	56, 2101
Asp700I	GAANNNNTTC	2 cut(s)	1143, 1436
AspLEI	GCGC	1 cut(s)	1915
AspS9I	GGNCC	8 cut(s)	89, 136, 202, 377, 1813, 2019, 2052, 2053
AsuC2I	CCSGG	1 cut(s)	98
AsuHPI	GGTGA	5 cut(s)	455, 982, 1430, 1643, 2279
AsuNHI	GCTAGC	1 cut(s)	1735
AvaI	CYCGRG	1 cut(s)	913
AvaII	GGWCC	4 cut(s)	89, 202, 377, 1813
BaeGI	GKGCMC	1 cut(s)	2056
BalI	TGGCCA	1 cut(s)	1847
BamHI	GGATCC	3 cut(s)	675, 2095, 2108
BanI	GGYRCC	1 cut(s)	1912
BanII	GRGCYC	4 cut(s)	452, 1989, 2056, 2330
BbsI	GAAGAC	2 cut(s)	1874, 2033
Bbv12I	GWGCWC	2 cut(s)	452, 1989
BbvI	GCAGC	6 cut(s)	494, 1256, 1496, 1582, 1603, 2213
BccI	CCATC	3 cut(s)	172, 2077, 2179
BceAI	ACGGC	1 cut(s)	2213
BciT130I	CCWGG	4 cut(s)	140, 1416, 2331, 2402
BciVI	GTATCC	1 cut(s)	1898
BclI	TGATCA	1 cut(s)	2142
BcnI	CCSGG	1 cut(s)	98
BcoDI	GTCTC	5 cut(s)	1146, 1574, 1773, 2179, 2330
BfaI	CTAG	6 cut(s)	422, 1530, 1736, 1805, 2045, 2090
BfmI	CTRYAG	2 cut(s)	1458, 2199
BfoI	RGCGCY	1 cut(s)	1916
BfrI	CTTAAG	2 cut(s)	403, 436
BfuI	GTATCC	1 cut(s)	1898
BglI	GCCNNNNNGGC	1 cut(s)	1702
BglII	AGATCT	4 cut(s)	568, 706, 1524, 1555
BisI	GCNGC	7 cut(s)	508, 694, 1245, 1510, 1596, 1617, 2202
BlpI	GCTNAGC	1 cut(s)	1099
BlsI	GCNGC	7 cut(s)	509, 695, 1246, 1511, 1597, 1618, 2203
Bme1390I	CCNGG	5 cut(s)	98, 140, 1416, 2331, 2402
Bme18I	GGWCC	4 cut(s)	89, 202, 377, 1813
BmeRI	GACNNNNNGTC	1 cut(s)	1159
BmeT110I	CYCGRG	1 cut(s)	913
BmgT120I	GGNCC	8 cut(s)	89, 136, 202, 377, 1813, 2019, 2052, 2053
BmiI	GGNNCC	9 cut(s)	379, 677, 1814, 1897, 1914, 2054, 2097, 2110, 2345
BmrFI	CCNGG	5 cut(s)	98, 140, 1416, 2331, 2402
BmrI	ACTGGG	2 cut(s)	669, 2285
BmsI	GCATC	6 cut(s)	345, 606, 897, 1603, 2311, 2368
BmtI	GCTAGC	1 cut(s)	1739
BmuI	ACTGGG	2 cut(s)	669, 2285
BpiI	GAAGAC	2 cut(s)	1874, 2033
BpmI	CTGGAG	2 cut(s)	769, 2160
Bpu10I	CCTNAGC	1 cut(s)	1472
Bpu1102I	GCTNAGC	1 cut(s)	1099
BpuEI	CTTGAG	1 cut(s)	2374
BpuMI	CCSGG	1 cut(s)	98
BsaBI	GATNNNNATC	1 cut(s)	2301
BsaHI	GRCGYC	1 cut(s)	1913
BsaI	GGTCTC	2 cut(s)	1574, 2179
BsaJI	CCNNGG	3 cut(s)	138, 912, 2329
Bsc4I	CCNNNNNNNGG	8 cut(s)	38, 108, 133, 179, 1358, 1673, 1750, 2105
Bse1I	ACTGG	9 cut(s)	307, 656, 664, 972, 1218, 1570, 1692, 1697, 2280
Bse3DI	GCAATG	3 cut(s)	845, 1722, 2139
Bse8I	GATNNNNATC	1 cut(s)	2301
BseBI	CCWGG	4 cut(s)	140, 1416, 2331, 2402
BseDI	CCNNGG	3 cut(s)	138, 912, 2329
BseGI	GGATG	3 cut(s)	597, 910, 2357
BseJI	GATNNNNATC	1 cut(s)	2301
BseLI	CCNNNNNNNGG	8 cut(s)	38, 108, 133, 179, 1358, 1673, 1750, 2105
BseMI	GCAATG	3 cut(s)	845, 1722, 2139
BseMII	CTCAG	4 cut(s)	82, 465, 1162, 2174
BseNI	ACTGG	9 cut(s)	307, 656, 664, 972, 1218, 1570, 1692, 1697, 2280
BseSI	GKGCMC	1 cut(s)	2056
BseXI	GCAGC	6 cut(s)	494, 1256, 1496, 1582, 1603, 2213
BseYI	CCCAGC	1 cut(s)	1328
BsgI	GTGCAG	1 cut(s)	2146
BshFI	GGCC	8 cut(s)	101, 137, 1696, 1847, 2020, 2054, 2223, 2288
BshNI	GGYRCC	1 cut(s)	1912
BsiHKAI	GWGCWC	2 cut(s)	452, 1989
BsiHKCI	CYCGRG	1 cut(s)	913
BsiSI	CCGG	2 cut(s)	98, 173
BslFI	GGGAC	6 cut(s)	725, 746, 1334, 1351, 1375, 1826
BslI	CCNNNNNNNGG	8 cut(s)	38, 108, 133, 179, 1358, 1673, 1750, 2105
BsmAI	GTCTC	5 cut(s)	1146, 1574, 1773, 2179, 2330
BsmFI	GGGAC	6 cut(s)	725, 746, 1334, 1351, 1375, 1826
BsmI	GAATGC	1 cut(s)	1491
BsnI	GGCC	8 cut(s)	101, 137, 1696, 1847, 2020, 2054, 2223, 2288
Bso31I	GGTCTC	2 cut(s)	1574, 2179
BsoBI	CYCGRG	1 cut(s)	913
Bsp120I	GGGCCC	1 cut(s)	2052
Bsp1286I	GDGCHC	4 cut(s)	452, 1989, 2056, 2330
Bsp1407I	TGTACA	1 cut(s)	1047
Bsp1720I	GCTNAGC	1 cut(s)	1099
BspACI	CCGC	3 cut(s)	170, 694, 1412
BspANI	GGCC	8 cut(s)	101, 137, 1696, 1847, 2020, 2054, 2223, 2288
BspCNI	CTCAG	4 cut(s)	81, 464, 1161, 2175
BspHI	TCATGA	1 cut(s)	2236
BspLI	GGNNCC	9 cut(s)	379, 677, 1814, 1897, 1914, 2054, 2097, 2110, 2345
BspMAI	CTGCAG	1 cut(s)	2203
BspOI	GCTAGC	1 cut(s)	1739
BspPI	GGATC	8 cut(s)	529, 670, 683, 1306, 2090, 2103, 2103, 2116
BspT107I	GGYRCC	1 cut(s)	1912
BspTI	CTTAAG	2 cut(s)	403, 436
BspTNI	GGTCTC	2 cut(s)	1574, 2179
BsrDI	GCAATG	3 cut(s)	845, 1722, 2139
BsrGI	TGTACA	1 cut(s)	1047
BsrI	ACTGG	9 cut(s)	307, 656, 664, 972, 1218, 1570, 1692, 1697, 2280
BssECI	CCNNGG	3 cut(s)	138, 912, 2329
BssNI	GRCGYC	1 cut(s)	1913
Bst2UI	CCWGG	4 cut(s)	140, 1416, 2331, 2402
Bst4CI	ACNGT	8 cut(s)	237, 840, 946, 1120, 1651, 1964, 2033, 2398
Bst6I	CTCTTC	2 cut(s)	1857, 2389
BstACI	GRCGYC	1 cut(s)	1913
BstAFI	CTTAAG	2 cut(s)	403, 436
BstAUI	TGTACA	1 cut(s)	1047
BstC8I	GCNNGC	4 cut(s)	360, 557, 1737, 2213
BstDEI	CTNAG	9 cut(s)	14, 68, 326, 451, 572, 1099, 1148, 1472, 2183
BstEII	GGTNACC	1 cut(s)	1934
BstENI	CCTNNNNNAGG	1 cut(s)	1748
BstF5I	GGATG	3 cut(s)	597, 910, 2357
BstH2I	RGCGCY	1 cut(s)	1916
BstHHI	GCGC	1 cut(s)	1915
BstMAI	GTCTC	5 cut(s)	1146, 1574, 1773, 2179, 2330
BstMWI	GCNNNNNNNGC	4 cut(s)	661, 1004, 1518, 1702
BstNI	CCWGG	4 cut(s)	140, 1416, 2331, 2402
BstNSI	RCATGY	3 cut(s)	487, 753, 2215
BstPI	GGTNACC	1 cut(s)	1934
BstSCI	CCNGG	5 cut(s)	96, 138, 1414, 2329, 2400
BstSFI	CTRYAG	2 cut(s)	1458, 2199
BstSLI	GKGCMC	1 cut(s)	2056
BstV1I	GCAGC	6 cut(s)	494, 1256, 1496, 1582, 1603, 2213
BstV2I	GAAGAC	2 cut(s)	1874, 2033
BstX2I	RGATCY	8 cut(s)	568, 675, 706, 1311, 1524, 1555, 2095, 2108
BstXI	CCANNNNNNTGG	1 cut(s)	36
BstYI	RGATCY	8 cut(s)	568, 675, 706, 1311, 1524, 1555, 2095, 2108
BsuI	GTATCC	1 cut(s)	1898
BsuRI	GGCC	8 cut(s)	101, 137, 1696, 1847, 2020, 2054, 2223, 2288
BtsCI	GGATG	3 cut(s)	597, 910, 2357
BtsI	GCAGTG	1 cut(s)	2153
BtsIMutI	CAGTG	7 cut(s)	76, 300, 436, 965, 1116, 2153, 2180
Cac8I	GCNNGC	4 cut(s)	360, 557, 1737, 2213
CciI	TCATGA	1 cut(s)	2236
CfoI	GCGC	1 cut(s)	1915
Cfr13I	GGNCC	8 cut(s)	89, 136, 202, 377, 1813, 2019, 2052, 2053
CseI	GACGC	2 cut(s)	894, 1879
Csp6I	GTAC	1 cut(s)	1048
CviQI	GTAC	1 cut(s)	1048
DdeI	CTNAG	9 cut(s)	14, 68, 326, 451, 572, 1099, 1148, 1472, 2183
DinI	GGCGCC	1 cut(s)	1914
DraI	TTTAAA	1 cut(s)	1282
DriI	GACNNNNNGTC	1 cut(s)	1159
EaeI	YGGCCR	1 cut(s)	1845
Eam1104I	CTCTTC	2 cut(s)	1857, 2389
Eam1105I	GACNNNNNGTC	1 cut(s)	1159
EarI	CTCTTC	2 cut(s)	1857, 2389
EciI	GGCGGA	1 cut(s)	159
Ecl136II	GAGCTC	2 cut(s)	450, 1987
Eco147I	AGGCCT	1 cut(s)	2288
Eco24I	GRGCYC	4 cut(s)	452, 1989, 2056, 2330
Eco31I	GGTCTC	2 cut(s)	1574, 2179
Eco47I	GGWCC	4 cut(s)	89, 202, 377, 1813
Eco53kI	GAGCTC	2 cut(s)	450, 1987
Eco57I	CTGAAG	1 cut(s)	326
Eco88I	CYCGRG	1 cut(s)	913
Eco91I	GGTNACC	1 cut(s)	1934
EcoICRI	GAGCTC	2 cut(s)	450, 1987
EcoNI	CCTNNNNNAGG	1 cut(s)	1748
EcoO109I	RGGNCCY	5 cut(s)	89, 202, 377, 1813, 2053
EcoO65I	GGTNACC	1 cut(s)	1934
EcoRII	CCWGG	4 cut(s)	138, 1414, 2329, 2400
EcoT38I	GRGCYC	4 cut(s)	452, 1989, 2056, 2330
EgeI	GGCGCC	1 cut(s)	1914
EheI	GGCGCC	1 cut(s)	1914
FaqI	GGGAC	6 cut(s)	725, 746, 1334, 1351, 1375, 1826
FauI	CCCGC	1 cut(s)	1419
FauNDI	CATATG	1 cut(s)	1366
FbaI	TGATCA	1 cut(s)	2142
FblI	GTMKAC	1 cut(s)	1953
Fnu4HI	GCNGC	7 cut(s)	508, 694, 1245, 1510, 1596, 1617, 2202
FokI	GGATG	3 cut(s)	584, 917, 2344
FriOI	GRGCYC	4 cut(s)	452, 1989, 2056, 2330
Fsp4HI	GCNGC	7 cut(s)	508, 694, 1245, 1510, 1596, 1617, 2202
FspBI	CTAG	6 cut(s)	422, 1530, 1736, 1805, 2045, 2090
GlaI	GCGC	1 cut(s)	1914
GluI	GCNGC	7 cut(s)	508, 694, 1245, 1510, 1596, 1617, 2202
GsaI	CCCAGC	1 cut(s)	1332
GsuI	CTGGAG	2 cut(s)	769, 2160
HaeII	RGCGCY	1 cut(s)	1916
HaeIII	GGCC	8 cut(s)	101, 137, 1696, 1847, 2020, 2054, 2223, 2288
HapII	CCGG	2 cut(s)	98, 173
HgaI	GACGC	2 cut(s)	894, 1879
HhaI	GCGC	1 cut(s)	1915
Hin1I	GRCGYC	1 cut(s)	1913
Hin6I	GCGC	1 cut(s)	1913
HinP1I	GCGC	1 cut(s)	1913
HindIII	AAGCTT	1 cut(s)	1625
HinfI	GANTC	8 cut(s)	604, 734, 1397, 1578, 1754, 2240, 2338, 2426
HpaII	CCGG	2 cut(s)	98, 173
HphI	GGTGA	5 cut(s)	455, 982, 1430, 1643, 2279
Hpy166II	GTNNAC	6 cut(s)	76, 1654, 1667, 1679, 1954, 1960
Hpy188I	TCNGA	9 cut(s)	128, 345, 642, 1042, 1151, 1402, 1834, 2113, 2343
Hpy8I	GTNNAC	6 cut(s)	76, 1654, 1667, 1679, 1954, 1960
HpyAV	CCTTC	7 cut(s)	221, 368, 396, 1216, 1628, 1667, 2213
HpyCH4III	ACNGT	8 cut(s)	237, 840, 946, 1120, 1651, 1964, 2033, 2398
HpyCH4IV	ACGT	2 cut(s)	347, 1956
HpyF10VI	GCNNNNNNNGC	4 cut(s)	661, 1004, 1518, 1702
HpyF3I	CTNAG	9 cut(s)	14, 68, 326, 451, 572, 1099, 1148, 1472, 2183
HpySE526I	ACGT	2 cut(s)	347, 1956
Hsp92I	GRCGYC	1 cut(s)	1913
HspAI	GCGC	1 cut(s)	1913
KasI	GGCGCC	1 cut(s)	1912
Ksp22I	TGATCA	1 cut(s)	2142
LmnI	GCTCC	5 cut(s)	504, 594, 923, 2086, 2343
Lsp1109I	GCAGC	6 cut(s)	494, 1256, 1496, 1582, 1603, 2213
LweI	GCATC	6 cut(s)	345, 606, 897, 1603, 2311, 2368
MaeI	CTAG	6 cut(s)	422, 1530, 1736, 1805, 2045, 2090
MaeII	ACGT	2 cut(s)	347, 1956
MaeIII	GTNAC	5 cut(s)	290, 431, 628, 647, 1934
MboII	GAAGA	6 cut(s)	1871, 1874, 1879, 2033, 2138, 2376
MfeI	CAATTG	2 cut(s)	811, 1392
MflI	RGATCY	8 cut(s)	568, 675, 706, 1311, 1524, 1555, 2095, 2108
MhlI	GDGCHC	4 cut(s)	452, 1989, 2056, 2330
MlsI	TGGCCA	1 cut(s)	1847
MluNI	TGGCCA	1 cut(s)	1847
Mly113I	GGCGCC	1 cut(s)	1913
MlyI	GAGTC	3 cut(s)	728, 1406, 2332
MmeI	TCCRAC	3 cut(s)	311, 1156, 1429
Mox20I	TGGCCA	1 cut(s)	1847
MroXI	GAANNNNTTC	2 cut(s)	1143, 1436
MscI	TGGCCA	1 cut(s)	1847
MslI	CAYNNNNRTG	3 cut(s)	34, 257, 1787
Msp20I	TGGCCA	1 cut(s)	1847
MspCI	CTTAAG	2 cut(s)	403, 436
MspI	CCGG	2 cut(s)	98, 173
MspR9I	CCNGG	5 cut(s)	98, 140, 1416, 2331, 2402
MunI	CAATTG	2 cut(s)	811, 1392
Mva1269I	GAATGC	1 cut(s)	1491
MvaI	CCWGG	4 cut(s)	140, 1416, 2331, 2402
MwoI	GCNNNNNNNGC	4 cut(s)	661, 1004, 1518, 1702
NarI	GGCGCC	1 cut(s)	1913
NciI	CCSGG	1 cut(s)	98
NdeI	CATATG	1 cut(s)	1366
NheI	GCTAGC	1 cut(s)	1735
NlaIV	GGNNCC	9 cut(s)	379, 677, 1814, 1897, 1914, 2054, 2097, 2110, 2345
NmeAIII	GCCGAG	1 cut(s)	2249
NmuCI	GTSAC	2 cut(s)	431, 628
NspI	RCATGY	3 cut(s)	487, 753, 2215
PaeI	GCATGC	1 cut(s)	2215
PagI	TCATGA	1 cut(s)	2236
PceI	AGGCCT	1 cut(s)	2288
PciI	ACATGT	1 cut(s)	749
PctI	GAATGC	1 cut(s)	1491
PdmI	GAANNNNTTC	2 cut(s)	1143, 1436
PfeI	GAWTC	5 cut(s)	604, 1578, 1754, 2240, 2426
PflFI	GACNNNGTC	1 cut(s)	737
PfoI	TCCNGGA	1 cut(s)	2400
PkrI	GCNGC	7 cut(s)	509, 695, 1246, 1511, 1597, 1618, 2203
PleI	GAGTC	3 cut(s)	728, 1405, 2332
PluTI	GGCGCC	1 cut(s)	1916
PpsI	GAGTC	3 cut(s)	728, 1405, 2332
PpuMI	RGGWCCY	4 cut(s)	89, 202, 377, 1813
PscI	ACATGT	1 cut(s)	749
Psp124BI	GAGCTC	2 cut(s)	452, 1989
Psp5II	RGGWCCY	4 cut(s)	89, 202, 377, 1813
Psp6I	CCWGG	4 cut(s)	138, 1414, 2329, 2400
PspEI	GGTNACC	1 cut(s)	1934
PspFI	CCCAGC	1 cut(s)	1328
PspGI	CCWGG	4 cut(s)	138, 1414, 2329, 2400
PspN4I	GGNNCC	9 cut(s)	379, 677, 1814, 1897, 1914, 2054, 2097, 2110, 2345
PspOMI	GGGCCC	1 cut(s)	2052
PspPI	GGNCC	8 cut(s)	89, 136, 202, 377, 1813, 2019, 2052, 2053
PspPPI	RGGWCCY	4 cut(s)	89, 202, 377, 1813
PstI	CTGCAG	1 cut(s)	2203
PsuI	RGATCY	8 cut(s)	568, 675, 706, 1311, 1524, 1555, 2095, 2108
PsyI	GACNNNGTC	1 cut(s)	737
RsaI	GTAC	1 cut(s)	1049
RsaNI	GTAC	1 cut(s)	1048
RseI	CAYNNNNRTG	3 cut(s)	34, 257, 1787
SacI	GAGCTC	2 cut(s)	452, 1989
SatI	GCNGC	7 cut(s)	508, 694, 1245, 1510, 1596, 1617, 2202
Sau96I	GGNCC	8 cut(s)	89, 136, 202, 377, 1813, 2019, 2052, 2053
SchI	GAGTC	3 cut(s)	728, 1406, 2332
ScrFI	CCNGG	5 cut(s)	98, 140, 1416, 2331, 2402
SduI	GDGCHC	4 cut(s)	452, 1989, 2056, 2330
SfaNI	GCATC	6 cut(s)	345, 606, 897, 1603, 2311, 2368
SfcI	CTRYAG	2 cut(s)	1458, 2199
SfoI	GGCGCC	1 cut(s)	1914
SinI	GGWCC	4 cut(s)	89, 202, 377, 1813
SmiMI	CAYNNNNRTG	3 cut(s)	34, 257, 1787
SmlI	CTYRAG	3 cut(s)	403, 436, 2389
SmoI	CTYRAG	3 cut(s)	403, 436, 2389
SphI	GCATGC	1 cut(s)	2215
SseBI	AGGCCT	1 cut(s)	2288
SsiI	CCGC	3 cut(s)	170, 694, 1412
SspDI	GGCGCC	1 cut(s)	1912
SspI	AATATT	4 cut(s)	1015, 1234, 1437, 2317
SspMI	CTAG	6 cut(s)	422, 1530, 1736, 1805, 2045, 2090
SstI	GAGCTC	2 cut(s)	452, 1989
StuI	AGGCCT	1 cut(s)	2288
StyD4I	CCNGG	5 cut(s)	96, 138, 1414, 2329, 2400
TaaI	ACNGT	8 cut(s)	237, 840, 946, 1120, 1651, 1964, 2033, 2398
TaiI	ACGT	2 cut(s)	350, 1959
TaqI	TCGA	3 cut(s)	214, 470, 754
TatI	WGTACW	1 cut(s)	1047
TauI	GCSGC	1 cut(s)	696
TfiI	GAWTC	5 cut(s)	604, 1578, 1754, 2240, 2426
TscAI	CASTG	7 cut(s)	76, 307, 436, 972, 1123, 2160, 2187
TseFI	GTSAC	2 cut(s)	431, 628
TseI	GCWGC	6 cut(s)	507, 1244, 1509, 1595, 1616, 2201
Tsp45I	GTSAC	2 cut(s)	431, 628
TspGWI	ACGGA	1 cut(s)	1304
TspRI	CASTG	7 cut(s)	76, 307, 436, 972, 1123, 2160, 2187
Tth111I	GACNNNGTC	1 cut(s)	737
Vha464I	CTTAAG	2 cut(s)	403, 436
VpaK11BI	GGWCC	4 cut(s)	89, 202, 377, 1813
XagI	CCTNNNNNAGG	1 cut(s)	1748
XapI	RAATTY	2 cut(s)	56, 2101
XbaI	TCTAGA	1 cut(s)	2044
XceI	RCATGY	3 cut(s)	487, 753, 2215
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1906
XmiI	GTMKAC	1 cut(s)	1953
XmnI	GAANNNNTTC	2 cut(s)	1143, 1436
XspI	CTAG	6 cut(s)	422, 1530, 1736, 1805, 2045, 2090

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.