FvH4_6g44190

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000114
Physical Location & Seq
Reverse (-)
34067215 .. 34070339
3125 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44190.t1

Sequence Viewer

Length: 2445 bp
ATGGGGTGGCTCTTGTGTTACCTAGTAACACTGCTCTTATCTCATATCCTGTCATTATGTACTTGCCAAGACAAATTAGTAGCAGGACAATATATCACTGGTAATCAAACTTTGGTTTCTTCCCTTGGGACATTTTCATTAGGCTTCTTCAATCCTGAGAATTCTAGTAAATACTTCCTTGGTATACGATTCAACACCTTCCCAGACACTGCGTTAGTATGGATTGCTAACAGAGAATCTCCGCTTGATTCTCCGGGTCTGTTTATGCTCAGCGGCGATGGAAACCTTGTAGTGTTGGATCAGACTGAAAATATTGTCTGGTCAACTAATGCATCACTATCTGCTTCTGCAATGAATCACACAACTGGATTACTAGCAGATACGGGAAACCTTGTTCTTAGCTTTGGAGAAGTCACCTTGTGGCAGAGCTTCAATCATCCCTCTGATACTTTGTTGCCAGACATGAAGATTAGCTTGAACAAAAAGACTGGCCAACGAACTGGCCTTACATCCTGGGCAGCGCTTGATGATCCACAACTTGGAAACTTCACCTACGGCATTGATAGTCAAGTACCACTTCAGAGCTTTATCTGGAAGGAAACTCTTCCGTATTTTAGAAGTTCTGTGTTCGTTGGCAAGGATACAAGAACAGTCTTTCAAATTCCAGGTGGAACCGCGATTTTCTTTTCATACAACACTGATGCTGATGAGGTTTATGGTGTTTTCAGTGTCTCGAATAGCTCGGTGAAATTCAGGGTCTGGTTGAGTCCTACAGGGCAACTTAAACAGCTGCTGTGGCAGGGTTCTAGTAAAACTTGGTTGGAACTTGAGACTTTACCTTCTGATAACTGTGATTTTTATGCTCATTGCGGTCCCAATAGTGCGTGTAGTAGAGGTGATTCCCTGCTGTCGTCAACACCATGCAAGTGTTTGACTGATTTTAAAGCCAAGTTTCCGAACCAATCGGTGGAGGGGGATTGGTCCGGGGGCTGCATTAGGGAAAAGGTGTTGGCATGTGGTAATGGCATACAGGATAACTTTTCAAGGCTTGAAAACGTGAAACTGCCGGATCATTCTGTTGTGTTAAACAATAAGACTTATAAGAGTATGAGCGAGTGTGAGTCGGAATGTCAGCAGAGCTGTTCTTGCACAGCTTATGCTTATGTAAATGGGACAGATGGAAGCACTGGGAAATGTTTAGCATGGTTTGGCGAATTACAGGATCTAGTAGAGACTAAAATTATTGCTGCAAATGATATTCGTATTCGTGTTCACGGGTCTGAATTAGGCAAAAAGGGTGTTTCTGTTAATTCCTTAAAGAGGACCCTTGTAATTGCAATAGTCTCTGCTGCAGTTGGACTGCTTACAATAACTTCTGGCTATTTCTTATGGAAGAAAAAATTGGGGAGGATAACAAGAACAGTAAGTGAGAATTTAGGTGGGAAGAATGATACAGAACTACCACTCTTCAGTTTAAGGAGTATATTAGCTGCCACAAACAACTTCTCTGAAGACAATAAACTTGGGGAGGGAGGATTTGGCCCTGTTTATAAGGGAATTTTGCCGGGAAATCAAGAAGTGGCCGTAAAGAGGCTATCAAAGAAGTCTGGGCAAGGGCATCATGAGTTCATGAATGAGTTGAAACTTATAGCCAAACTCCAGCATACCAACCTTGCTCATCTCATGGGTTGCTGCACGGAAGGAGATGAACTGATATTGATCTACGAGTACATGCCTAATCGAAGTTTGGACAAATTTTTGTTTGATCCTTTTGAAAAGACAAAGTTGGACTGGGCTACACGGTTTCGAATTATACAAGGTATTGCTCAAGGAGTACTATATATCCACAAATACTCTAGGTTGAAAATCATCCATAGAGATTTAAAAGCAAGTAATGTTCTGTTGGATGGAATGATGAATCCCAAAGTGTCTGACTTTGGAATGGCGAGGATTTTTGATATAAATCAAATTGAAGCAAATACCAACAAGGTTGTTGGGACATACGGCTACATGTCACCTGAATATGCAGTATATGGTCATTTTTCTGGGAAATTGGATGTGTTCAGTTTTGGAGTACTATTGTTAGAGATTGTAAGCGGAAAGAAGAATGCTTTGTTTTATTCTTGCGAAAATTCACAAACGCTAGCTCAATGGATATGGCAACTATGGAAAGAAGGTAGAGGAGTGGAGGTAATTGACGCATCAGTAAGGGAAACTTGCCGGATTCATGAAGCTTTGAGGTGCATCCATGTCGGGCTTTTGTGTGTTCAAGAAGCTCCAGCTGATCGACCGACAATGTCTTTAGTGATTCATATGCTCGAGGCTGAAGAAGCTACCTCACTTCCACCCTCCAAAGAACCTGCGTTTTCAACAAGTAGGAATTCAAGTTCTGTTACCACTTATTCCAAAAATGGAGTCACTATTACTTTGCCAGAACCTCGATAG

Protein Analysis

815

Amino Acids

89.99

Weight (kDa)

6.61

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 172 3.3e-29 D-mannose binding lectin
S_locus_glycop PF00954 228 - 317 2.2e-15 S-locus glycoprotein domain
PAN_2 PF08276 343 - 408 2.4e-15 PAN-like domain
Pkinase PF00069 503 - 769 5.9e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 505 - 771 7.5e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000114)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_4g01160 FvH4_6g34940 FvH4_6g44062 FvH4_6g44106 FvH4_6g44106 FvH4_6g44106 FvH4_6g44107 FvH4_6g44107 FvH4_6g44109 FvH4_6g44140 FvH4_6g44190 FvH4_6g44200 FvH4_6g44240 FvH4_6g44241 FvH4_6g44242
malus_domestica MD09G1094900.v1.1 MD09G1100300.v1.1 MD09G1100400.v1.1 MD09G1100600.v1.1 MD09G1100800.v1.1 MD09G1100900.v1.1 MD09G1101300.v1.1 MD09G1255200.v1.1 MD17G1088400.v1.1 MD17G1088600.v1.1 MD17G1247900.v1.1 MD17G1248300.v1.1
prunus_persica Prupe.1G497000_v2.0.a1 Prupe.2G033300_v2.0.a1 Prupe.3G145500_v2.0.a1 Prupe.3G225400_v2.0.a1 Prupe.3G225700_v2.0.a1 Prupe.3G225700_v2.0.a1
pyrus_communis pycom06g12720 pycom06g12740 pycom09g01960 pycom09g02450 pycom17g08520 pycom17g08530 pycom17g08560 pycom17g24950 pycom17g25030 pycom17g25110 pycom17g25260 pycom17g25290
rosa_chinensis RchiOBHm_Chr1g0326521 RchiOBHm_Chr1g0326551 RchiOBHm_Chr1g0326591 RchiOBHm_Chr1g0328741 RchiOBHm_Chr1g0329371 RchiOBHm_Chr2g0145911 RchiOBHm_Chr2g0160891 RchiOBHm_Chr2g0160921 RchiOBHm_Chr2g0161001 RchiOBHm_Chr2g0161011 RchiOBHm_Chr2g0161431 RchiOBHm_Chr2g0165121 RchiOBHm_Chr2g0166301 RchiOBHm_Chr4g0399551 RchiOBHm_Chr4g0405681 RchiOBHm_Chr5g0066741 RchiOBHm_Chr5g0066841 RchiOBHm_Chr6g0273021 RchiOBHm_Chr7g0225151
rosa_laevigata RLG00000001843 RLG00000008827 RLG00000014283 RLG00000014284 RLG00000020173 RLG00000021244 RLG00000021246 RLG00000021248 RLG00000021283 RLG00000023606 RLG00000029921 RLG00000030066 RLG00000030067 RLG00000033456
rosa_multiflora Rmu_co7958161.1_g000001 Rmu_co8440151.1_g000001 Rmu_co8454989.1_g000001 Rmu_sc0000129.1_g000018 Rmu_sc0000322.1_g000011 Rmu_sc0000322.1_g000045 Rmu_sc0000605.1_g000051 Rmu_sc0000955.1_g000015 Rmu_sc0000955.1_g000020 Rmu_sc0001188.1_g000017 Rmu_sc0001188.1_g000019 Rmu_sc0001355.1_g000015 Rmu_sc0001702.1_g000005 Rmu_sc0001702.1_g000006 Rmu_sc0001702.1_g000007 Rmu_sc0001702.1_g000008 Rmu_sc0001702.1_g000009 Rmu_sc0001702.1_g000010 Rmu_sc0001702.1_g000011 Rmu_sc0001702.1_g000012 Rmu_sc0001702.1_g000013 Rmu_sc0001702.1_g000014 Rmu_sc0001702.1_g000015 Rmu_sc0001702.1_g000016 Rmu_sc0002473.1_g000012 Rmu_sc0002473.1_g000015 Rmu_sc0002773.1_g000029 Rmu_sc0004178.1_g000002 Rmu_sc0005189.1_g000006 Rmu_sc0005860.1_g000004 Rmu_sc0009205.1_g000005 Rmu_sc0009205.1_g000007 Rmu_sc0009205.1_g000023 Rmu_sc0009472.1_g000005 Rmu_sc0009858.1_g000015 Rmu_sc0020187.1_g000001
rosa_roxburghii Rroxscaffold_176G00431460 Rroxscaffold_176G00431480 Rroxscaffold_1G00014330 Rroxscaffold_1G00031590 Rroxscaffold_2G00088820 Rroxscaffold_2G00089330 Rroxscaffold_2G00089360 Rroxscaffold_2G00089370 Rroxscaffold_2G00089380 Rroxscaffold_2G00100370 Rroxscaffold_2G00100380 Rroxscaffold_4G00321410 Rroxscaffold_4G00323700 Rroxscaffold_4G00327640 Rroxscaffold_4G00327650 Rroxscaffold_5G00344450 Rroxscaffold_7G00202770 Rroxscaffold_7G00203450 Rroxscaffold_7G00203540
rosa_rugosa Rorug01G0061100 Rorug02G0482100 Rorug02G0485900 Rorug02G0485900 Rorug02G0489500 Rorug02G0489600 Rorug02G0489700 Rorug04G0016600 Rorug04G0016700 Rorug05G0379000 Rorug05G0516700 Rorug06G0007900 Rorug06G0009300
rosa_samantha Rh1AG076700 Rh1AG076800 Rh1AG077000 Rh1AG077300 Rh1AG094400 Rh1AG097500 Rh1AG402000 Rh1BG062700 Rh1BG062900 Rh1CG075500 Rh1CG075600 Rh1CG075800 Rh1CG090900 Rh1CG091400 Rh1CG091600 Rh1CG094100 Rh1DG082300 Rh1DG082500 Rh1DG097400 Rh2AG448200 Rh2AG448300 Rh2AG511600 Rh2AG547500 Rh2AG551400 Rh2AG551700 Rh2AG555800 Rh2AG588000 Rh2BG565900 Rh2BG566000 Rh2BG566300 Rh2BG569000 Rh2BG600000 Rh2CG435200 Rh2CG435300 Rh2CG496600 Rh2CG535400 Rh2CG535600 Rh2CG539700 Rh2CG562700 Rh2CG570900 Rh2DG574600 Rh2DG574800 Rh2DG578600 Rh2DG610200 Rh4CG140900 Rh4DG127800 Rh5AG438500 Rh5BG436500 Rh5BG455500 Rh5CG477600 Rh5DG470300 Rh6AG129500 Rh6AG129900 Rh6BG125700 Rh6BG126000 Rh6BG126700 Rh6BG126800 Rh6CG124500 Rh6CG124800 Rh6CG125200 Rh6CG125300 Rh6DG111400 Rh6DG111700 Rh6DG111800 Rh7DG373300
rosa_wichuraiana Rw0G014010 Rw1G006140 Rw1G006160 Rw1G006180 Rw1G006190 Rw1G007340 Rw1G007360 Rw2G045660 Rw2G045670 Rw2G045970 Rw2G048220 Rw4G010820 Rw5G041010 Rw6G011220 Rw6G011230 Rw7G031490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1101, 1551
Acc36I ACCTGC 1 cut(s) 2368
AccB7I CCANNNNNTGG 2 cut(s) 539, 967
AccI GTMKAC 1 cut(s) 184
AccII CGCG 1 cut(s) 677
AciI CCGC 5 cut(s) 242, 273, 675, 870, 2097
AclWI GGATC 5 cut(s) 306, 524, 1077, 1230, 1760
AcoI YGGCCR 2 cut(s) 490, 1581
AcsI RAATTY 8 cut(s) 160, 660, 749, 1432, 1557, 1754, 2131, 2380
AcuI CTGAAG 4 cut(s) 563, 1453, 1530, 2346
AdeI CACNNNGTG 1 cut(s) 420
AfaI GTAC 5 cut(s) 61, 573, 1730, 1836, 2076
AfeI AGCGCT 1 cut(s) 522
AfiI CCNNNNNNNGG 4 cut(s) 539, 967, 1320, 1590
AflIII ACRYGT 1 cut(s) 2010
AjnI CCWGG 2 cut(s) 512, 664
AjuI GAANNNNNNNTTGG 2 cut(s) 1385, 1417
AloI GAACNNNNNNTCC 4 cut(s) 378, 410, 2371, 2403
Alw26I GTCTC 4 cut(s) 736, 824, 1226, 1348
AlwI GGATC 5 cut(s) 306, 524, 1077, 1230, 1760
AlwNI CAGNNNCTG 3 cut(s) 209, 759, 793
Ama87I CYCGRG 1 cut(s) 2318
Aor51HI AGCGCT 1 cut(s) 522
AoxI GGCC 4 cut(s) 490, 502, 1540, 1581
ApeKI GCWGC 7 cut(s) 518, 790, 990, 1247, 1349, 1490, 1692
ApoI RAATTY 8 cut(s) 160, 660, 749, 1432, 1557, 1754, 2131, 2380
ArsI GACNNNNNNTTYG 2 cut(s) 2400, 2432
Asp700I GAANNNNTTC 1 cut(s) 603
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 4 cut(s) 872, 981, 1323, 1541
AsuC2I CCSGG 3 cut(s) 255, 985, 1566
AsuHPI GGTGA 5 cut(s) 406, 541, 757, 908, 2007
AsuII TTCGAA 1 cut(s) 1807
AsuNHI GCTAGC 1 cut(s) 2143
AvaI CYCGRG 1 cut(s) 2318
AvaII GGWCC 3 cut(s) 872, 981, 1323
BalI TGGCCA 1 cut(s) 492
BbsI GAAGAC 1 cut(s) 1518
BbvI GCAGC 7 cut(s) 530, 777, 977, 1234, 1336, 1477, 1679
BccI CCATC 3 cut(s) 272, 1172, 1901
BceAI ACGGC 3 cut(s) 571, 1568, 2020
BcgI CGANNNNNNTGC 2 cut(s) 2233, 2267
BciT130I CCWGG 2 cut(s) 514, 666
BciVI GTATCC 1 cut(s) 634
BcnI CCSGG 3 cut(s) 255, 985, 1566
BcoDI GTCTC 4 cut(s) 736, 824, 1226, 1348
BfaI CTAG 7 cut(s) 23, 165, 374, 807, 1226, 1857, 2144
BfmI CTRYAG 2 cut(s) 771, 1350
BfoI RGCGCY 1 cut(s) 524
BfuAI ACCTGC 1 cut(s) 2368
BfuI GTATCC 1 cut(s) 634
BisI GCNGC 8 cut(s) 274, 519, 791, 991, 1248, 1350, 1491, 1693
BlpI GCTNAGC 1 cut(s) 269
BlsI GCNGC 8 cut(s) 275, 520, 792, 992, 1249, 1351, 1492, 1694
BmcAI AGTACT 2 cut(s) 1836, 2076
Bme1390I CCNGG 5 cut(s) 255, 514, 666, 985, 1566
Bme18I GGWCC 3 cut(s) 872, 981, 1323
BmeT110I CYCGRG 1 cut(s) 2318
BmgT120I GGNCC 4 cut(s) 872, 981, 1323, 1541
BmiI GGNNCC 3 cut(s) 673, 874, 1325
BmrFI CCNGG 5 cut(s) 255, 514, 666, 985, 1566
BmrI ACTGGG 2 cut(s) 1197, 1801
BmsI GCATC 5 cut(s) 341, 691, 1627, 2210, 2253
BmtI GCTAGC 1 cut(s) 2147
BmuI ACTGGG 2 cut(s) 1197, 1801
BpiI GAAGAC 1 cut(s) 1518
BpmI CTGGAG 2 cut(s) 1643, 2262
Bpu1102I GCTNAGC 1 cut(s) 269
Bpu14I TTCGAA 1 cut(s) 1807
BpuEI CTTGAG 2 cut(s) 848, 1812
BpuMI CCSGG 3 cut(s) 255, 985, 1566
BsaBI GATNNNNATC 2 cut(s) 1718, 1962
BsaJI CCNNGG 4 cut(s) 124, 178, 513, 984
BsaXI ACNNNNNCTCC 2 cut(s) 2175, 2205
Bsc4I CCNNNNNNNGG 4 cut(s) 539, 967, 1320, 1590
Bse1I ACTGG 6 cut(s) 103, 370, 493, 505, 1192, 1796
Bse3DI GCAATG 2 cut(s) 357, 865
Bse8I GATNNNNATC 2 cut(s) 1718, 1962
BseBI CCWGG 2 cut(s) 514, 666
BseDI CCNNGG 4 cut(s) 124, 178, 513, 984
BseGI GGATG 6 cut(s) 436, 509, 1869, 1912, 2062, 2244
BseJI GATNNNNATC 2 cut(s) 1718, 1962
BseLI CCNNNNNNNGG 4 cut(s) 539, 967, 1320, 1590
BseMI GCAATG 2 cut(s) 357, 865
BseMII CTCAG 2 cut(s) 147, 283
BseNI ACTGG 6 cut(s) 103, 370, 493, 505, 1192, 1796
BseRI GAGGAG 1 cut(s) 2196
BseXI GCAGC 7 cut(s) 530, 777, 977, 1234, 1336, 1477, 1679
BsgI GTGCAG 1 cut(s) 1678
Bsh1236I CGCG 1 cut(s) 677
Bsh1285I CGRYCG 1 cut(s) 2291
BshFI GGCC 4 cut(s) 492, 504, 1542, 1583
BsiEI CGRYCG 1 cut(s) 2291
BsiHKCI CYCGRG 1 cut(s) 2318
BsiSI CCGG 5 cut(s) 254, 984, 1067, 1565, 2221
BslFI GGGAC 4 cut(s) 142, 858, 1186, 2011
BslI CCNNNNNNNGG 4 cut(s) 539, 967, 1320, 1590
BsmAI GTCTC 4 cut(s) 736, 824, 1226, 1348
BsmFI GGGAC 4 cut(s) 142, 858, 1186, 2011
BsmI GAATGC 1 cut(s) 2113
BsnI GGCC 4 cut(s) 492, 504, 1542, 1583
BsoBI CYCGRG 1 cut(s) 2318
Bsp119I TTCGAA 1 cut(s) 1807
Bsp143I GATC 7 cut(s) 298, 529, 1069, 1222, 1719, 1765, 2284
Bsp1720I GCTNAGC 1 cut(s) 269
BspACI CCGC 5 cut(s) 242, 273, 675, 870, 2097
BspANI GGCC 4 cut(s) 492, 504, 1542, 1583
BspCNI CTCAG 2 cut(s) 148, 282
BspFNI CGCG 1 cut(s) 677
BspHI TCATGA 3 cut(s) 1621, 1629, 2227
BspLI GGNNCC 3 cut(s) 673, 874, 1325
BspMAI CTGCAG 1 cut(s) 1354
BspMI ACCTGC 1 cut(s) 2368
BspOI GCTAGC 1 cut(s) 2147
BspPI GGATC 5 cut(s) 306, 524, 1077, 1230, 1760
BspT104I TTCGAA 1 cut(s) 1807
BsrDI GCAATG 2 cut(s) 357, 865
BsrI ACTGG 6 cut(s) 103, 370, 493, 505, 1192, 1796
BssECI CCNNGG 4 cut(s) 124, 178, 513, 984
BssMI GATC 7 cut(s) 298, 529, 1069, 1222, 1719, 1765, 2284
BssNAI GTATAC 1 cut(s) 185
BssT1I CCWWGG 2 cut(s) 124, 178
Bst1107I GTATAC 1 cut(s) 185
Bst2UI CCWGG 2 cut(s) 514, 666
Bst4CI ACNGT 4 cut(s) 652, 851, 1423, 1803
Bst6I CTCTTC 2 cut(s) 609, 1472
BstBI TTCGAA 1 cut(s) 1807
BstC8I GCNNGC 1 cut(s) 2145
BstDEI CTNAG 3 cut(s) 156, 269, 398
BstENI CCTNNNNNAGG 1 cut(s) 1318
BstF5I GGATG 6 cut(s) 436, 509, 1869, 1912, 2062, 2244
BstFNI CGCG 1 cut(s) 677
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 1 cut(s) 523
BstKTI GATC 7 cut(s) 301, 532, 1072, 1225, 1722, 1768, 2287
BstMAI GTCTC 4 cut(s) 736, 824, 1226, 1348
BstMBI GATC 7 cut(s) 298, 529, 1069, 1222, 1719, 1765, 2284
BstMCI CGRYCG 1 cut(s) 2291
BstMWI GCNNNNNNNGC 3 cut(s) 796, 1146, 2330
BstNI CCWGG 2 cut(s) 514, 666
BstNSI RCATGY 3 cut(s) 1017, 1735, 2014
BstSCI CCNGG 5 cut(s) 253, 512, 664, 983, 1564
BstSFI CTRYAG 2 cut(s) 771, 1350
BstUI CGCG 1 cut(s) 677
BstV1I GCAGC 7 cut(s) 530, 777, 977, 1234, 1336, 1477, 1679
BstV2I GAAGAC 1 cut(s) 1518
BstX2I RGATCY 1 cut(s) 1222
BstXI CCANNNNNNTGG 1 cut(s) 500
BstYI RGATCY 1 cut(s) 1222
BstZ17I GTATAC 1 cut(s) 185
BsuI GTATCC 1 cut(s) 634
BsuRI GGCC 4 cut(s) 492, 504, 1542, 1583
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 6 cut(s) 436, 509, 1869, 1912, 2062, 2244
BtsI GCAGTG 2 cut(s) 29, 207
BtsIMutI CAGTG 6 cut(s) 29, 96, 207, 696, 733, 1185
BveI ACCTGC 1 cut(s) 2368
Cac8I GCNNGC 1 cut(s) 2145
CaiI CAGNNNCTG 3 cut(s) 209, 759, 793
CciI TCATGA 3 cut(s) 1621, 1629, 2227
CfoI GCGC 1 cut(s) 523
Cfr13I GGNCC 4 cut(s) 872, 981, 1323, 1541
CseI GACGC 1 cut(s) 2207
Csp6I GTAC 5 cut(s) 60, 572, 1729, 1835, 2075
CviQI GTAC 5 cut(s) 60, 572, 1729, 1835, 2075
DdeI CTNAG 3 cut(s) 156, 269, 398
DpnI GATC 7 cut(s) 300, 531, 1071, 1224, 1721, 1767, 2286
DpnII GATC 7 cut(s) 298, 529, 1069, 1222, 1719, 1765, 2284
DraI TTTAAA 2 cut(s) 943, 1884
DraIII CACNNNGTG 1 cut(s) 420
EaeI YGGCCR 2 cut(s) 490, 1581
Eam1104I CTCTTC 2 cut(s) 609, 1472
EarI CTCTTC 2 cut(s) 609, 1472
Eco130I CCWWGG 2 cut(s) 124, 178
Eco47I GGWCC 3 cut(s) 872, 981, 1323
Eco47III AGCGCT 1 cut(s) 522
Eco57I CTGAAG 4 cut(s) 563, 1453, 1530, 2346
Eco88I CYCGRG 1 cut(s) 2318
EcoNI CCTNNNNNAGG 1 cut(s) 1318
EcoO109I RGGNCCY 1 cut(s) 1323
EcoRI GAATTC 2 cut(s) 160, 2380
EcoRII CCWGG 2 cut(s) 512, 664
EcoT14I CCWWGG 2 cut(s) 124, 178
EcoT22I ATGCAT 1 cut(s) 334
ErhI CCWWGG 2 cut(s) 124, 178
FalI AAGNNNNNCTT 6 cut(s) 458, 490, 561, 593, 2200, 2232
FaqI GGGAC 4 cut(s) 142, 858, 1186, 2011
FauNDI CATATG 1 cut(s) 2313
FblI GTMKAC 1 cut(s) 184
Fnu4HI GCNGC 8 cut(s) 274, 519, 791, 991, 1248, 1350, 1491, 1693
FokI GGATG 6 cut(s) 423, 496, 1856, 1919, 2069, 2231
Fsp4HI GCNGC 8 cut(s) 274, 519, 791, 991, 1248, 1350, 1491, 1693
FspBI CTAG 7 cut(s) 23, 165, 374, 807, 1226, 1857, 2144
GlaI GCGC 1 cut(s) 522
GluI GCNGC 8 cut(s) 274, 519, 791, 991, 1248, 1350, 1491, 1693
GsuI CTGGAG 2 cut(s) 1643, 2262
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 4 cut(s) 492, 504, 1542, 1583
HapII CCGG 5 cut(s) 254, 984, 1067, 1565, 2221
HgaI GACGC 1 cut(s) 2207
HhaI GCGC 1 cut(s) 523
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HincII GTYRAC 2 cut(s) 324, 915
HindII GTYRAC 2 cut(s) 324, 915
HindIII AAGCTT 1 cut(s) 2232
HpaII CCGG 5 cut(s) 254, 984, 1067, 1565, 2221
HphI GGTGA 5 cut(s) 406, 541, 757, 908, 2007
Hpy166II GTNNAC 4 cut(s) 185, 324, 915, 1273
Hpy188I TCNGA 9 cut(s) 303, 445, 582, 844, 957, 1126, 1282, 1510, 1933
Hpy188III TCNNGA 8 cut(s) 155, 592, 733, 1574, 1622, 1630, 2228, 2270
Hpy8I GTNNAC 4 cut(s) 185, 324, 915, 1273
HpyAV CCTTC 5 cut(s) 208, 589, 849, 1694, 2168
HpyCH4III ACNGT 4 cut(s) 652, 851, 1423, 1803
HpyCH4IV ACGT 1 cut(s) 1056
HpyF10VI GCNNNNNNNGC 3 cut(s) 796, 1146, 2330
HpyF3I CTNAG 3 cut(s) 156, 269, 398
HpySE526I ACGT 1 cut(s) 1056
HspAI GCGC 1 cut(s) 521
Kzo9I GATC 7 cut(s) 298, 529, 1069, 1222, 1719, 1765, 2284
LmnI GCTCC 1 cut(s) 2281
Lsp1109I GCAGC 7 cut(s) 530, 777, 977, 1234, 1336, 1477, 1679
LweI GCATC 5 cut(s) 341, 691, 1627, 2210, 2253
MaeI CTAG 7 cut(s) 23, 165, 374, 807, 1226, 1857, 2144
MaeII ACGT 1 cut(s) 1056
MaeIII GTNAC 6 cut(s) 17, 25, 412, 2013, 2392, 2416
MalI GATC 7 cut(s) 300, 531, 1071, 1224, 1721, 1767, 2286
MboI GATC 7 cut(s) 298, 529, 1069, 1222, 1719, 1765, 2284
MflI RGATCY 1 cut(s) 1222
MlsI TGGCCA 1 cut(s) 492
MluNI TGGCCA 1 cut(s) 492
MlyI GAGTC 3 cut(s) 775, 1130, 2424
MmeI TCCRAC 6 cut(s) 276, 801, 1104, 1336, 1767, 1884
Mox20I TGGCCA 1 cut(s) 492
Mph1103I ATGCAT 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 603
MscI TGGCCA 1 cut(s) 492
MseI TTAA 7 cut(s) 783, 942, 1085, 1308, 1316, 1475, 1883
MslI CAYNNNNRTG 1 cut(s) 925
Msp20I TGGCCA 1 cut(s) 492
MspA1I CMGCKG 3 cut(s) 273, 790, 2282
MspI CCGG 5 cut(s) 254, 984, 1067, 1565, 2221
MspR9I CCNGG 5 cut(s) 255, 514, 666, 985, 1566
Mva1269I GAATGC 1 cut(s) 2113
MvaI CCWGG 2 cut(s) 514, 666
MvnI CGCG 1 cut(s) 677
MwoI GCNNNNNNNGC 3 cut(s) 796, 1146, 2330
NciI CCSGG 3 cut(s) 255, 985, 1566
NdeI CATATG 1 cut(s) 2313
NdeII GATC 7 cut(s) 298, 529, 1069, 1222, 1719, 1765, 2284
NheI GCTAGC 1 cut(s) 2143
NlaIV GGNNCC 3 cut(s) 673, 874, 1325
NmuCI GTSAC 3 cut(s) 412, 2013, 2416
NsiI ATGCAT 1 cut(s) 334
NspI RCATGY 3 cut(s) 1017, 1735, 2014
NspV TTCGAA 1 cut(s) 1807
PaeR7I CTCGAG 1 cut(s) 2318
PagI TCATGA 3 cut(s) 1621, 1629, 2227
PciI ACATGT 1 cut(s) 2010
PctI GAATGC 1 cut(s) 2113
PdmI GAANNNNTTC 1 cut(s) 603
PfeI GAWTC 8 cut(s) 189, 236, 248, 355, 899, 1918, 2224, 2308
PflFI GACNNNGTC 1 cut(s) 2296
PflMI CCANNNNNTGG 2 cut(s) 539, 967
PkrI GCNGC 8 cut(s) 275, 520, 792, 992, 1249, 1351, 1492, 1694
PleI GAGTC 3 cut(s) 774, 1129, 2423
PpsI GAGTC 3 cut(s) 774, 1129, 2423
PpuMI RGGWCCY 1 cut(s) 1323
PscI ACATGT 1 cut(s) 2010
PsiI TTATAA 2 cut(s) 1101, 1551
Psp5II RGGWCCY 1 cut(s) 1323
Psp6I CCWGG 2 cut(s) 512, 664
PspGI CCWGG 2 cut(s) 512, 664
PspN4I GGNNCC 3 cut(s) 673, 874, 1325
PspPI GGNCC 4 cut(s) 872, 981, 1323, 1541
PspPPI RGGWCCY 1 cut(s) 1323
PspXI VCTCGAGB 1 cut(s) 2318
PsrI GAACNNNNNNTAC 2 cut(s) 490, 522
PstI CTGCAG 1 cut(s) 1354
PstNI CAGNNNCTG 3 cut(s) 209, 759, 793
PsuI RGATCY 1 cut(s) 1222
PsyI GACNNNGTC 1 cut(s) 2296
PvuII CAGCTG 2 cut(s) 790, 2282
RsaI GTAC 5 cut(s) 61, 573, 1730, 1836, 2076
RsaNI GTAC 5 cut(s) 60, 572, 1729, 1835, 2075
RseI CAYNNNNRTG 1 cut(s) 925
SaqAI TTAA 7 cut(s) 783, 942, 1085, 1308, 1316, 1475, 1883
SatI GCNGC 8 cut(s) 274, 519, 791, 991, 1248, 1350, 1491, 1693
Sau3AI GATC 7 cut(s) 298, 529, 1069, 1222, 1719, 1765, 2284
Sau96I GGNCC 4 cut(s) 872, 981, 1323, 1541
ScaI AGTACT 2 cut(s) 1836, 2076
SchI GAGTC 3 cut(s) 775, 1130, 2424
ScrFI CCNGG 5 cut(s) 255, 514, 666, 985, 1566
SfaNI GCATC 5 cut(s) 341, 691, 1627, 2210, 2253
SfcI CTRYAG 2 cut(s) 771, 1350
Sfr274I CTCGAG 1 cut(s) 2318
SfuI TTCGAA 1 cut(s) 1807
SinI GGWCC 3 cut(s) 872, 981, 1323
SlaI CTCGAG 1 cut(s) 2318
SmiMI CAYNNNNRTG 1 cut(s) 925
SmlI CTYRAG 3 cut(s) 827, 1827, 2318
SmoI CTYRAG 3 cut(s) 827, 1827, 2318
SsiI CCGC 5 cut(s) 242, 273, 675, 870, 2097
SspI AATATT 1 cut(s) 313
SspMI CTAG 7 cut(s) 23, 165, 374, 807, 1226, 1857, 2144
StyD4I CCNGG 5 cut(s) 253, 512, 664, 983, 1564
StyI CCWWGG 2 cut(s) 124, 178
TaaI ACNGT 4 cut(s) 652, 851, 1423, 1803
TaiI ACGT 1 cut(s) 1059
TaqI TCGA 6 cut(s) 734, 1741, 1807, 2287, 2319, 2440
TaqII GACCGA 1 cut(s) 2305
TatI WGTACW 4 cut(s) 59, 1728, 1834, 2074
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 8 cut(s) 189, 236, 248, 355, 899, 1918, 2224, 2308
Tru1I TTAA 7 cut(s) 783, 942, 1085, 1308, 1316, 1475, 1883
Tru9I TTAA 7 cut(s) 783, 942, 1085, 1308, 1316, 1475, 1883
TscAI CASTG 6 cut(s) 36, 103, 214, 703, 733, 1192
TseFI GTSAC 3 cut(s) 412, 2013, 2416
TseI GCWGC 7 cut(s) 518, 790, 990, 1247, 1349, 1490, 1692
Tsp45I GTSAC 3 cut(s) 412, 2013, 2416
TspGWI ACGGA 2 cut(s) 597, 1712
TspRI CASTG 6 cut(s) 36, 103, 214, 703, 733, 1192
Tth111I GACNNNGTC 1 cut(s) 2296
Van91I CCANNNNNTGG 2 cut(s) 539, 967
VpaK11BI GGWCC 3 cut(s) 872, 981, 1323
XagI CCTNNNNNAGG 1 cut(s) 1318
XapI RAATTY 8 cut(s) 160, 660, 749, 1432, 1557, 1754, 2131, 2380
XceI RCATGY 3 cut(s) 1017, 1735, 2014
XhoI CTCGAG 1 cut(s) 2318
XmiI GTMKAC 1 cut(s) 184
XmnI GAANNNNTTC 1 cut(s) 603
XspI CTAG 7 cut(s) 23, 165, 374, 807, 1226, 1857, 2144
ZrmI AGTACT 2 cut(s) 1836, 2076
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.