Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 128 |
| AccII | CGCG | 1 cut(s) | 126 |
| AciI | CCGC | 4 cut(s) | 126, 141, 144, 962 |
| AclWI | GGATC | 3 cut(s) | 596, 609, 953 |
| AcsI | RAATTY | 5 cut(s) | 312, 829, 861, 879, 895 |
| AcuI | CTGAAG | 1 cut(s) | 480 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 85, 214, 215 |
| AflIII | ACRYGT | 1 cut(s) | 459 |
| AgsI | TTSAA | 6 cut(s) | 25, 722, 727, 828, 921, 927 |
| AjnI | CCWGG | 1 cut(s) | 696 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 407, 439 |
| AluBI | AGCT | 3 cut(s) | 500, 595, 659 |
| AluI | AGCT | 3 cut(s) | 500, 595, 659 |
| Alw26I | GTCTC | 2 cut(s) | 56, 572 |
| AlwI | GGATC | 3 cut(s) | 596, 609, 953 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 659, 662 |
| AoxI | GGCC | 4 cut(s) | 149, 449, 615, 768 |
| ApeKI | GCWGC | 6 cut(s) | 121, 538, 653, 656, 659, 1008 |
| ApoI | RAATTY | 5 cut(s) | 312, 829, 861, 879, 895 |
| AspLEI | GCGC | 2 cut(s) | 126, 670 |
| AspS9I | GGNCC | 4 cut(s) | 97, 449, 975, 984 |
| AsuHPI | GGTGA | 1 cut(s) | 63 |
| AvaII | GGWCC | 3 cut(s) | 97, 975, 984 |
| AxyI | CCTNAGG | 2 cut(s) | 161, 772 |
| BamHI | GGATCC | 1 cut(s) | 601 |
| BanI | GGYRCC | 1 cut(s) | 128 |
| BbvI | GCAGC | 6 cut(s) | 133, 550, 646, 665, 668, 995 |
| BccI | CCATC | 4 cut(s) | 613, 704, 986, 995 |
| BciT130I | CCWGG | 1 cut(s) | 698 |
| BclI | TGATCA | 1 cut(s) | 285 |
| BcoDI | GTCTC | 2 cut(s) | 56, 572 |
| BfaI | CTAG | 1 cut(s) | 528 |
| BfmI | CTRYAG | 1 cut(s) | 651 |
| BisI | GCNGC | 8 cut(s) | 122, 142, 145, 539, 654, 657, 660, 1009 |
| BlpI | GCTNAGC | 1 cut(s) | 591 |
| BlsI | GCNGC | 8 cut(s) | 123, 143, 146, 540, 655, 658, 661, 1010 |
| Bme1390I | CCNGG | 1 cut(s) | 698 |
| Bme18I | GGWCC | 3 cut(s) | 97, 975, 984 |
| BmgT120I | GGNCC | 4 cut(s) | 97, 449, 975, 984 |
| BmiI | GGNNCC | 6 cut(s) | 98, 130, 603, 642, 976, 985 |
| BmrFI | CCNGG | 1 cut(s) | 698 |
| BmsI | GCATC | 4 cut(s) | 452, 467, 655, 691 |
| BpmI | CTGGAG | 1 cut(s) | 858 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 591 |
| BpuEI | CTTGAG | 1 cut(s) | 118 |
| BsaI | GGTCTC | 1 cut(s) | 56 |
| BsaJI | CCNNGG | 1 cut(s) | 612 |
| BsaWI | WCCGGW | 1 cut(s) | 643 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 331, 361 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 85, 214, 215 |
| Bse118I | RCCGGY | 1 cut(s) | 958 |
| Bse1I | ACTGG | 2 cut(s) | 751, 903 |
| Bse21I | CCTNAGG | 2 cut(s) | 161, 772 |
| BseBI | CCWGG | 1 cut(s) | 698 |
| BseDI | CCNNGG | 1 cut(s) | 612 |
| BseGI | GGATG | 3 cut(s) | 482, 706, 899 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 85, 214, 215 |
| BseMII | CTCAG | 4 cut(s) | 51, 387, 786, 821 |
| BseNI | ACTGG | 2 cut(s) | 751, 903 |
| BseXI | GCAGC | 6 cut(s) | 133, 550, 646, 665, 668, 995 |
| BsgI | GTGCAG | 1 cut(s) | 752 |
| Bsh1236I | CGCG | 1 cut(s) | 126 |
| BshFI | GGCC | 4 cut(s) | 151, 451, 617, 770 |
| BshNI | GGYRCC | 1 cut(s) | 128 |
| BsiSI | CCGG | 2 cut(s) | 644, 959 |
| BslFI | GGGAC | 3 cut(s) | 263, 988, 997 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 85, 214, 215 |
| BsmAI | GTCTC | 2 cut(s) | 56, 572 |
| BsmBI | CGTCTC | 1 cut(s) | 572 |
| BsmFI | GGGAC | 3 cut(s) | 263, 988, 997 |
| BsnI | GGCC | 4 cut(s) | 151, 451, 617, 770 |
| Bso31I | GGTCTC | 1 cut(s) | 56 |
| Bsp143I | GATC | 3 cut(s) | 285, 601, 945 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 591 |
| BspACI | CCGC | 4 cut(s) | 126, 141, 144, 962 |
| BspANI | GGCC | 4 cut(s) | 151, 451, 617, 770 |
| BspCNI | CTCAG | 4 cut(s) | 52, 388, 785, 820 |
| BspFNI | CGCG | 1 cut(s) | 126 |
| BspLI | GGNNCC | 6 cut(s) | 98, 130, 603, 642, 976, 985 |
| BspMAI | CTGCAG | 1 cut(s) | 655 |
| BspPI | GGATC | 3 cut(s) | 596, 609, 953 |
| BspT107I | GGYRCC | 1 cut(s) | 128 |
| BspTNI | GGTCTC | 1 cut(s) | 56 |
| BsrFI | RCCGGY | 1 cut(s) | 958 |
| BsrI | ACTGG | 2 cut(s) | 751, 903 |
| BssAI | RCCGGY | 1 cut(s) | 958 |
| BssECI | CCNNGG | 1 cut(s) | 612 |
| BssMI | GATC | 3 cut(s) | 285, 601, 945 |
| BssT1I | CCWWGG | 1 cut(s) | 612 |
| Bst2UI | CCWGG | 1 cut(s) | 698 |
| Bst4CI | ACNGT | 5 cut(s) | 239, 355, 361, 556, 562 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 659, 762 |
| BstC8I | GCNNGC | 4 cut(s) | 149, 293, 956, 960 |
| BstDEI | CTNAG | 6 cut(s) | 60, 161, 396, 591, 772, 807 |
| BstF5I | GGATG | 3 cut(s) | 482, 706, 899 |
| BstFNI | CGCG | 1 cut(s) | 126 |
| BstHHI | GCGC | 2 cut(s) | 126, 670 |
| BstKTI | GATC | 3 cut(s) | 288, 604, 948 |
| BstMAI | GTCTC | 2 cut(s) | 56, 572 |
| BstMBI | GATC | 3 cut(s) | 285, 601, 945 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 118, 497, 659, 665, 762 |
| BstNI | CCWGG | 1 cut(s) | 698 |
| BstNSI | RCATGY | 3 cut(s) | 267, 295, 958 |
| BstSCI | CCNGG | 1 cut(s) | 696 |
| BstSFI | CTRYAG | 1 cut(s) | 651 |
| BstUI | CGCG | 1 cut(s) | 126 |
| BstV1I | GCAGC | 6 cut(s) | 133, 550, 646, 665, 668, 995 |
| BstX2I | RGATCY | 1 cut(s) | 601 |
| BstYI | RGATCY | 1 cut(s) | 601 |
| Bsu36I | CCTNAGG | 2 cut(s) | 161, 772 |
| BsuRI | GGCC | 4 cut(s) | 151, 451, 617, 770 |
| BtsCI | GGATG | 3 cut(s) | 482, 706, 899 |
| Cac8I | GCNNGC | 4 cut(s) | 149, 293, 956, 960 |
| CaiI | CAGNNNCTG | 2 cut(s) | 659, 662 |
| CfoI | GCGC | 2 cut(s) | 126, 670 |
| Cfr10I | RCCGGY | 1 cut(s) | 958 |
| Cfr13I | GGNCC | 4 cut(s) | 97, 449, 975, 984 |
| CseI | GACGC | 2 cut(s) | 65, 589 |
| CviAII | CATG | 5 cut(s) | 264, 292, 322, 543, 955 |
| DdeI | CTNAG | 6 cut(s) | 60, 161, 396, 591, 772, 807 |
| DpnI | GATC | 3 cut(s) | 287, 603, 947 |
| DpnII | GATC | 3 cut(s) | 285, 601, 945 |
| Eco130I | CCWWGG | 1 cut(s) | 612 |
| Eco31I | GGTCTC | 1 cut(s) | 56 |
| Eco47I | GGWCC | 3 cut(s) | 97, 975, 984 |
| Eco57I | CTGAAG | 1 cut(s) | 480 |
| Eco81I | CCTNAGG | 2 cut(s) | 161, 772 |
| EcoRI | GAATTC | 1 cut(s) | 312 |
| EcoRII | CCWGG | 1 cut(s) | 696 |
| EcoT14I | CCWWGG | 1 cut(s) | 612 |
| ErhI | CCWWGG | 1 cut(s) | 612 |
| Esp3I | CGTCTC | 1 cut(s) | 572 |
| FaeI | CATG | 5 cut(s) | 267, 295, 325, 546, 958 |
| FaqI | GGGAC | 3 cut(s) | 263, 988, 997 |
| FatI | CATG | 5 cut(s) | 263, 291, 321, 542, 954 |
| FauI | CCCGC | 1 cut(s) | 955 |
| FbaI | TGATCA | 1 cut(s) | 285 |
| Fnu4HI | GCNGC | 8 cut(s) | 122, 142, 145, 539, 654, 657, 660, 1009 |
| FokI | GGATG | 3 cut(s) | 489, 713, 886 |
| Fsp4HI | GCNGC | 8 cut(s) | 122, 142, 145, 539, 654, 657, 660, 1009 |
| FspBI | CTAG | 1 cut(s) | 528 |
| GlaI | GCGC | 2 cut(s) | 125, 669 |
| GluI | GCNGC | 8 cut(s) | 122, 142, 145, 539, 654, 657, 660, 1009 |
| GsuI | CTGGAG | 1 cut(s) | 858 |
| HaeIII | GGCC | 4 cut(s) | 151, 451, 617, 770 |
| HapII | CCGG | 2 cut(s) | 644, 959 |
| HgaI | GACGC | 2 cut(s) | 65, 589 |
| HhaI | GCGC | 2 cut(s) | 126, 670 |
| Hin1II | CATG | 5 cut(s) | 267, 295, 325, 546, 958 |
| Hin6I | GCGC | 2 cut(s) | 124, 668 |
| HinP1I | GCGC | 2 cut(s) | 124, 668 |
| HincII | GTYRAC | 1 cut(s) | 626 |
| HindII | GTYRAC | 1 cut(s) | 626 |
| HindIII | AAGCTT | 1 cut(s) | 593 |
| HinfI | GANTC | 1 cut(s) | 68 |
| HpaII | CCGG | 2 cut(s) | 644, 959 |
| HphI | GGTGA | 1 cut(s) | 63 |
| Hpy166II | GTNNAC | 1 cut(s) | 626 |
| Hpy188I | TCNGA | 3 cut(s) | 311, 351, 601 |
| Hpy188III | TCNNGA | 7 cut(s) | 135, 280, 395, 774, 857, 875, 982 |
| Hpy8I | GTNNAC | 1 cut(s) | 626 |
| HpyCH4III | ACNGT | 5 cut(s) | 239, 355, 361, 556, 562 |
| HpyCH4IV | ACGT | 1 cut(s) | 459 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 118, 497, 659, 665, 762 |
| HpyF3I | CTNAG | 6 cut(s) | 60, 161, 396, 591, 772, 807 |
| HpySE526I | ACGT | 1 cut(s) | 459 |
| Hsp92II | CATG | 5 cut(s) | 267, 295, 325, 546, 958 |
| HspAI | GCGC | 2 cut(s) | 124, 668 |
| KroI | GCCGGC | 1 cut(s) | 958 |
| KroNI | GCCGGC | 1 cut(s) | 960 |
| Ksp22I | TGATCA | 1 cut(s) | 285 |
| Kzo9I | GATC | 3 cut(s) | 285, 601, 945 |
| LmnI | GCTCC | 1 cut(s) | 218 |
| Lsp1109I | GCAGC | 6 cut(s) | 133, 550, 646, 665, 668, 995 |
| LweI | GCATC | 4 cut(s) | 452, 467, 655, 691 |
| MaeI | CTAG | 1 cut(s) | 528 |
| MaeII | ACGT | 1 cut(s) | 459 |
| MaeIII | GTNAC | 4 cut(s) | 69, 259, 355, 556 |
| MalI | GATC | 3 cut(s) | 287, 603, 947 |
| MboI | GATC | 3 cut(s) | 285, 601, 945 |
| MboII | GAAGA | 1 cut(s) | 4 |
| MfeI | CAATTG | 1 cut(s) | 760 |
| MflI | RGATCY | 1 cut(s) | 601 |
| MlyI | GAGTC | 1 cut(s) | 77 |
| MmeI | TCCRAC | 1 cut(s) | 374 |
| MnlI | CCTC | 8 cut(s) | 131, 142, 250, 428, 462, 628, 770, 781 |
| MroNI | GCCGGC | 1 cut(s) | 958 |
| MseI | TTAA | 3 cut(s) | 30, 318, 519 |
| MslI | CAYNNNNRTG | 1 cut(s) | 953 |
| MspA1I | CMGCKG | 1 cut(s) | 659 |
| MspI | CCGG | 2 cut(s) | 644, 959 |
| MspR9I | CCNGG | 1 cut(s) | 698 |
| MunI | CAATTG | 1 cut(s) | 760 |
| MvaI | CCWGG | 1 cut(s) | 698 |
| MvnI | CGCG | 1 cut(s) | 126 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 118, 497, 659, 665, 762 |
| NaeI | GCCGGC | 1 cut(s) | 960 |
| NdeII | GATC | 3 cut(s) | 285, 601, 945 |
| NgoMIV | GCCGGC | 1 cut(s) | 958 |
| NlaIII | CATG | 5 cut(s) | 267, 295, 325, 546, 958 |
| NlaIV | GGNNCC | 6 cut(s) | 98, 130, 603, 642, 976, 985 |
| NmuCI | GTSAC | 4 cut(s) | 69, 259, 355, 556 |
| NspI | RCATGY | 3 cut(s) | 267, 295, 958 |
| PaeI | GCATGC | 2 cut(s) | 295, 958 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 63 |
| PdiI | GCCGGC | 1 cut(s) | 960 |
| PflFI | GACNNNGTC | 2 cut(s) | 67, 275 |
| PfoI | TCCNGGA | 1 cut(s) | 696 |
| PkrI | GCNGC | 8 cut(s) | 123, 143, 146, 540, 655, 658, 661, 1010 |
| PleI | GAGTC | 1 cut(s) | 76 |
| PpsI | GAGTC | 1 cut(s) | 76 |
| Psp6I | CCWGG | 1 cut(s) | 696 |
| PspGI | CCWGG | 1 cut(s) | 696 |
| PspN4I | GGNNCC | 6 cut(s) | 98, 130, 603, 642, 976, 985 |
| PspPI | GGNCC | 4 cut(s) | 97, 449, 975, 984 |
| PstI | CTGCAG | 1 cut(s) | 655 |
| PstNI | CAGNNNCTG | 2 cut(s) | 659, 662 |
| PsuI | RGATCY | 1 cut(s) | 601 |
| PsyI | GACNNNGTC | 2 cut(s) | 67, 275 |
| PvuII | CAGCTG | 1 cut(s) | 659 |
| RseI | CAYNNNNRTG | 1 cut(s) | 953 |
| SaqAI | TTAA | 3 cut(s) | 30, 318, 519 |
| SatI | GCNGC | 8 cut(s) | 122, 142, 145, 539, 654, 657, 660, 1009 |
| Sau3AI | GATC | 3 cut(s) | 285, 601, 945 |
| Sau96I | GGNCC | 4 cut(s) | 97, 449, 975, 984 |
| SchI | GAGTC | 1 cut(s) | 77 |
| ScrFI | CCNGG | 1 cut(s) | 698 |
| SfaNI | GCATC | 4 cut(s) | 452, 467, 655, 691 |
| SfcI | CTRYAG | 1 cut(s) | 651 |
| SinI | GGWCC | 3 cut(s) | 97, 975, 984 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 953 |
| SmlI | CTYRAG | 1 cut(s) | 133 |
| SmoI | CTYRAG | 1 cut(s) | 133 |
| SphI | GCATGC | 2 cut(s) | 295, 958 |
| SsiI | CCGC | 4 cut(s) | 126, 141, 144, 962 |
| SspI | AATATT | 2 cut(s) | 930, 1029 |
| SspMI | CTAG | 1 cut(s) | 528 |
| StyD4I | CCNGG | 1 cut(s) | 696 |
| StyI | CCWWGG | 1 cut(s) | 612 |
| TaaI | ACNGT | 5 cut(s) | 239, 355, 361, 556, 562 |
| TaiI | ACGT | 1 cut(s) | 462 |
| TaqI | TCGA | 3 cut(s) | 15, 255, 485 |
| TaqII | GACCGA | 1 cut(s) | 80 |
| TauI | GCSGC | 2 cut(s) | 144, 147 |
| Tru1I | TTAA | 3 cut(s) | 30, 318, 519 |
| Tru9I | TTAA | 3 cut(s) | 30, 318, 519 |
| TseFI | GTSAC | 4 cut(s) | 69, 259, 355, 556 |
| TseI | GCWGC | 6 cut(s) | 121, 538, 653, 656, 659, 1008 |
| Tsp45I | GTSAC | 4 cut(s) | 69, 259, 355, 556 |
| TspDTI | ATGAA | 6 cut(s) | 338, 802, 822, 837, 888, 897 |
| TspGWI | ACGGA | 1 cut(s) | 120 |
| Tth111I | GACNNNGTC | 2 cut(s) | 67, 275 |
| VpaK11BI | GGWCC | 3 cut(s) | 97, 975, 984 |
| XapI | RAATTY | 5 cut(s) | 312, 829, 861, 879, 895 |
| XceI | RCATGY | 3 cut(s) | 267, 295, 958 |
| XspI | CTAG | 1 cut(s) | 528 |