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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 291 |
| Acc36I | ACCTGC | 1 cut(s) | 291 |
| AclWI | GGATC | 1 cut(s) | 117 |
| AcsI | RAATTY | 1 cut(s) | 206 |
| AcuI | CTGAAG | 1 cut(s) | 78 |
| AfaI | GTAC | 1 cut(s) | 184 |
| AflIII | ACRYGT | 1 cut(s) | 179 |
| AluBI | AGCT | 1 cut(s) | 242 |
| AluI | AGCT | 1 cut(s) | 242 |
| AlwI | GGATC | 1 cut(s) | 117 |
| AoxI | GGCC | 1 cut(s) | 266 |
| ApoI | RAATTY | 1 cut(s) | 206 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 243 |
| AspS9I | GGNCC | 2 cut(s) | 188, 267 |
| AsuHPI | GGTGA | 2 cut(s) | 272, 371 |
| AvaII | GGWCC | 1 cut(s) | 188 |
| BfuAI | ACCTGC | 1 cut(s) | 291 |
| Bme18I | GGWCC | 1 cut(s) | 188 |
| BmgT120I | GGNCC | 2 cut(s) | 188, 267 |
| BmiI | GGNNCC | 1 cut(s) | 189 |
| BpuEI | CTTGAG | 2 cut(s) | 234, 365 |
| Bsa29I | ATCGAT | 1 cut(s) | 121 |
| BsaAI | YACGTR | 1 cut(s) | 182 |
| BsaBI | GATNNNNATC | 1 cut(s) | 158 |
| Bse8I | GATNNNNATC | 1 cut(s) | 158 |
| BseCI | ATCGAT | 1 cut(s) | 121 |
| BseGI | GGATG | 1 cut(s) | 260 |
| BseJI | GATNNNNATC | 1 cut(s) | 158 |
| BsgI | GTGCAG | 1 cut(s) | 269 |
| Bsh1285I | CGRYCG | 2 cut(s) | 144, 162 |
| BshFI | GGCC | 1 cut(s) | 268 |
| BshVI | ATCGAT | 1 cut(s) | 121 |
| BsiEI | CGRYCG | 2 cut(s) | 144, 162 |
| BsiWI | CGTACG | 1 cut(s) | 182 |
| BsnI | GGCC | 1 cut(s) | 268 |
| Bsp143I | GATC | 3 cut(s) | 122, 141, 159 |
| BspANI | GGCC | 1 cut(s) | 268 |
| BspDI | ATCGAT | 1 cut(s) | 121 |
| BspHI | TCATGA | 1 cut(s) | 235 |
| BspLI | GGNNCC | 1 cut(s) | 189 |
| BspMI | ACCTGC | 1 cut(s) | 291 |
| BspPI | GGATC | 1 cut(s) | 117 |
| BssMI | GATC | 3 cut(s) | 122, 141, 159 |
| BstBAI | YACGTR | 1 cut(s) | 182 |
| BstF5I | GGATG | 1 cut(s) | 260 |
| BstKTI | GATC | 3 cut(s) | 125, 144, 162 |
| BstMBI | GATC | 3 cut(s) | 122, 141, 159 |
| BstMCI | CGRYCG | 2 cut(s) | 144, 162 |
| Bsu15I | ATCGAT | 1 cut(s) | 121 |
| BsuRI | GGCC | 1 cut(s) | 268 |
| BsuTUI | ATCGAT | 1 cut(s) | 121 |
| BtsCI | GGATG | 1 cut(s) | 260 |
| BtsI | GCAGTG | 1 cut(s) | 320 |
| BtsIMutI | CAGTG | 1 cut(s) | 320 |
| BveI | ACCTGC | 1 cut(s) | 291 |
| CciI | TCATGA | 1 cut(s) | 235 |
| Cfr13I | GGNCC | 2 cut(s) | 188, 267 |
| ClaI | ATCGAT | 1 cut(s) | 121 |
| Csp6I | GTAC | 1 cut(s) | 183 |
| CviAII | CATG | 1 cut(s) | 236 |
| CviJI | RGCY | 3 cut(s) | 242, 268, 298 |
| CviKI_1 | RGCY | 3 cut(s) | 242, 268, 298 |
| CviQI | GTAC | 1 cut(s) | 183 |
| DpnI | GATC | 3 cut(s) | 124, 143, 161 |
| DpnII | GATC | 3 cut(s) | 122, 141, 159 |
| Eco47I | GGWCC | 1 cut(s) | 188 |
| Eco57I | CTGAAG | 1 cut(s) | 78 |
| EcoO109I | RGGNCCY | 1 cut(s) | 188 |
| EcoRI | GAATTC | 1 cut(s) | 206 |
| FaeI | CATG | 1 cut(s) | 239 |
| FaiI | YATR | 3 cut(s) | 30, 71, 237 |
| FatI | CATG | 1 cut(s) | 235 |
| FokI | GGATG | 1 cut(s) | 267 |
| HaeIII | GGCC | 1 cut(s) | 268 |
| Hin1II | CATG | 1 cut(s) | 239 |
| HinfI | GANTC | 1 cut(s) | 118 |
| HphI | GGTGA | 2 cut(s) | 272, 371 |
| Hpy166II | GTNNAC | 1 cut(s) | 150 |
| Hpy188I | TCNGA | 1 cut(s) | 86 |
| Hpy188III | TCNNGA | 5 cut(s) | 96, 151, 203, 236, 344 |
| Hpy8I | GTNNAC | 1 cut(s) | 150 |
| HpyCH4IV | ACGT | 3 cut(s) | 16, 181, 273 |
| HpyCH4V | TGCA | 2 cut(s) | 13, 286 |
| HpySE526I | ACGT | 3 cut(s) | 16, 181, 273 |
| Hsp92II | CATG | 1 cut(s) | 239 |
| Kzo9I | GATC | 3 cut(s) | 122, 141, 159 |
| LpnPI | CCDG | 5 cut(s) | 23, 81, 188, 296, 342 |
| MaeII | ACGT | 3 cut(s) | 16, 181, 273 |
| MaeIII | GTNAC | 2 cut(s) | 88, 278 |
| MalI | GATC | 3 cut(s) | 124, 143, 161 |
| MboI | GATC | 3 cut(s) | 122, 141, 159 |
| MluCI | AATT | 3 cut(s) | 19, 206, 388 |
| MnlI | CCTC | 5 cut(s) | 92, 235, 258, 258, 385 |
| MroXI | GAANNNNTTC | 1 cut(s) | 243 |
| MseI | TTAA | 3 cut(s) | 300, 351, 387 |
| NdeII | GATC | 3 cut(s) | 122, 141, 159 |
| NlaIII | CATG | 1 cut(s) | 239 |
| NlaIV | GGNNCC | 1 cut(s) | 189 |
| NmuCI | GTSAC | 1 cut(s) | 278 |
| PagI | TCATGA | 1 cut(s) | 235 |
| PaqCI | CACCTGC | 1 cut(s) | 291 |
| PdmI | GAANNNNTTC | 1 cut(s) | 243 |
| PfeI | GAWTC | 1 cut(s) | 118 |
| Pfl23II | CGTACG | 1 cut(s) | 182 |
| Ple19I | CGATCG | 2 cut(s) | 144, 162 |
| Ppu21I | YACGTR | 1 cut(s) | 182 |
| PpuMI | RGGWCCY | 1 cut(s) | 188 |
| Psp5II | RGGWCCY | 1 cut(s) | 188 |
| PspLI | CGTACG | 1 cut(s) | 182 |
| PspN4I | GGNNCC | 1 cut(s) | 189 |
| PspPI | GGNCC | 2 cut(s) | 188, 267 |
| PspPPI | RGGWCCY | 1 cut(s) | 188 |
| PvuI | CGATCG | 2 cut(s) | 144, 162 |
| RsaI | GTAC | 1 cut(s) | 184 |
| RsaNI | GTAC | 1 cut(s) | 183 |
| SaqAI | TTAA | 3 cut(s) | 300, 351, 387 |
| Sau3AI | GATC | 3 cut(s) | 122, 141, 159 |
| Sau96I | GGNCC | 2 cut(s) | 188, 267 |
| SetI | ASST | 6 cut(s) | 19, 184, 244, 276, 285, 361 |
| SinI | GGWCC | 1 cut(s) | 188 |
| SmlI | CTYRAG | 2 cut(s) | 249, 344 |
| SmoI | CTYRAG | 2 cut(s) | 249, 344 |
| Sse9I | AATT | 3 cut(s) | 19, 206, 388 |
| TaiI | ACGT | 3 cut(s) | 19, 184, 276 |
| TaqI | TCGA | 2 cut(s) | 121, 158 |
| TasI | AATT | 3 cut(s) | 19, 206, 388 |
| TfiI | GAWTC | 1 cut(s) | 118 |
| Tru1I | TTAA | 3 cut(s) | 300, 351, 387 |
| Tru9I | TTAA | 3 cut(s) | 300, 351, 387 |
| TscAI | CASTG | 1 cut(s) | 320 |
| TseFI | GTSAC | 1 cut(s) | 278 |
| Tsp45I | GTSAC | 1 cut(s) | 278 |
| TspDTI | ATGAA | 2 cut(s) | 199, 252 |
| TspRI | CASTG | 1 cut(s) | 320 |
| VpaK11BI | GGWCC | 1 cut(s) | 188 |
| XapI | RAATTY | 1 cut(s) | 206 |
| XmnI | GAANNNNTTC | 1 cut(s) | 243 |