Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 560, 743 |
| AccII | CGCG | 1 cut(s) | 87 |
| AciI | CCGC | 2 cut(s) | 600, 608 |
| AclWI | GGATC | 2 cut(s) | 539, 872 |
| AcsI | RAATTY | 5 cut(s) | 4, 469, 752, 940, 952 |
| AcyI | GRCGYC | 1 cut(s) | 561 |
| AfaI | GTAC | 6 cut(s) | 292, 428, 529, 775, 799, 898 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 170, 861 |
| AflIII | ACRYGT | 3 cut(s) | 414, 794, 893 |
| AgsI | TTSAA | 3 cut(s) | 596, 662, 939 |
| AluBI | AGCT | 5 cut(s) | 524, 807, 830, 905, 935 |
| AluI | AGCT | 5 cut(s) | 524, 807, 830, 905, 935 |
| Alw26I | GTCTC | 1 cut(s) | 632 |
| AlwI | GGATC | 2 cut(s) | 539, 872 |
| AoxI | GGCC | 1 cut(s) | 688 |
| ApeKI | GCWGC | 2 cut(s) | 117, 993 |
| ApoI | RAATTY | 5 cut(s) | 4, 469, 752, 940, 952 |
| AspLEI | GCGC | 4 cut(s) | 87, 89, 563, 982 |
| AspS9I | GGNCC | 1 cut(s) | 1010 |
| AsuHPI | GGTGA | 3 cut(s) | 804, 844, 901 |
| AvaII | GGWCC | 1 cut(s) | 1010 |
| BanI | GGYRCC | 2 cut(s) | 560, 743 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 766, 798 |
| BbvI | GCAGC | 2 cut(s) | 129, 980 |
| BccI | CCATC | 4 cut(s) | 644, 823, 841, 1001 |
| BclI | TGATCA | 2 cut(s) | 48, 240 |
| BcoDI | GTCTC | 1 cut(s) | 632 |
| BfaI | CTAG | 3 cut(s) | 321, 525, 971 |
| BfmI | CTRYAG | 2 cut(s) | 115, 627 |
| BfoI | RGCGCY | 1 cut(s) | 564 |
| BglII | AGATCT | 1 cut(s) | 847 |
| BisI | GCNGC | 3 cut(s) | 118, 609, 994 |
| BlsI | GCNGC | 3 cut(s) | 119, 610, 995 |
| BmcAI | AGTACT | 3 cut(s) | 292, 529, 775 |
| Bme18I | GGWCC | 1 cut(s) | 1010 |
| BmgT120I | GGNCC | 1 cut(s) | 1010 |
| BmiI | GGNNCC | 3 cut(s) | 562, 585, 745 |
| BmrI | ACTGGG | 1 cut(s) | 343 |
| BmsI | GCATC | 4 cut(s) | 85, 422, 719, 967 |
| BmuI | ACTGGG | 1 cut(s) | 343 |
| BsaHI | GRCGYC | 1 cut(s) | 561 |
| BsaJI | CCNNGG | 1 cut(s) | 960 |
| BsaWI | WCCGGW | 1 cut(s) | 586 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 286, 316 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 170, 861 |
| Bse1I | ACTGG | 4 cut(s) | 338, 548, 691, 745 |
| Bse3DI | GCAATG | 1 cut(s) | 442 |
| BseDI | CCNNGG | 1 cut(s) | 960 |
| BseGI | GGATG | 4 cut(s) | 437, 673, 833, 956 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 170, 861 |
| BseMI | GCAATG | 1 cut(s) | 442 |
| BseNI | ACTGG | 4 cut(s) | 338, 548, 691, 745 |
| BsePI | GCGCGC | 1 cut(s) | 85 |
| BseXI | GCAGC | 2 cut(s) | 129, 980 |
| Bsh1236I | CGCG | 1 cut(s) | 87 |
| BshFI | GGCC | 1 cut(s) | 690 |
| BshNI | GGYRCC | 2 cut(s) | 560, 743 |
| BsiSI | CCGG | 1 cut(s) | 587 |
| BslFI | GGGAC | 2 cut(s) | 333, 521 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 170, 861 |
| BsmAI | GTCTC | 1 cut(s) | 632 |
| BsmFI | GGGAC | 2 cut(s) | 333, 521 |
| BsmI | GAATGC | 1 cut(s) | 273 |
| BsnI | GGCC | 1 cut(s) | 690 |
| Bsp143I | GATC | 6 cut(s) | 48, 66, 240, 544, 847, 864 |
| BspACI | CCGC | 2 cut(s) | 600, 608 |
| BspANI | GGCC | 1 cut(s) | 690 |
| BspFNI | CGCG | 1 cut(s) | 87 |
| BspLI | GGNNCC | 3 cut(s) | 562, 585, 745 |
| BspMAI | CTGCAG | 1 cut(s) | 119 |
| BspPI | GGATC | 2 cut(s) | 539, 872 |
| BspQI | GCTCTTC | 1 cut(s) | 814 |
| BspT107I | GGYRCC | 2 cut(s) | 560, 743 |
| BsrDI | GCAATG | 1 cut(s) | 442 |
| BsrI | ACTGG | 4 cut(s) | 338, 548, 691, 745 |
| BssECI | CCNNGG | 1 cut(s) | 960 |
| BssHII | GCGCGC | 1 cut(s) | 85 |
| BssMI | GATC | 6 cut(s) | 48, 66, 240, 544, 847, 864 |
| BssNI | GRCGYC | 1 cut(s) | 561 |
| BssT1I | CCWWGG | 1 cut(s) | 960 |
| Bst4CI | ACNGT | 7 cut(s) | 194, 310, 445, 454, 488, 913, 1028 |
| Bst6I | CTCTTC | 3 cut(s) | 392, 403, 814 |
| BstACI | GRCGYC | 1 cut(s) | 561 |
| BstC8I | GCNNGC | 1 cut(s) | 87 |
| BstDEI | CTNAG | 1 cut(s) | 77 |
| BstF5I | GGATG | 4 cut(s) | 437, 673, 833, 956 |
| BstFNI | CGCG | 1 cut(s) | 87 |
| BstH2I | RGCGCY | 1 cut(s) | 564 |
| BstHHI | GCGC | 4 cut(s) | 87, 89, 563, 982 |
| BstKTI | GATC | 6 cut(s) | 51, 69, 243, 547, 850, 867 |
| BstMAI | GTCTC | 1 cut(s) | 632 |
| BstMBI | GATC | 6 cut(s) | 48, 66, 240, 544, 847, 864 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 95, 489, 608, 702, 999 |
| BstNSI | RCATGY | 2 cut(s) | 222, 798 |
| BstSFI | CTRYAG | 2 cut(s) | 115, 627 |
| BstUI | CGCG | 1 cut(s) | 87 |
| BstV1I | GCAGC | 2 cut(s) | 129, 980 |
| BstX2I | RGATCY | 2 cut(s) | 847, 864 |
| BstYI | RGATCY | 2 cut(s) | 847, 864 |
| BsuRI | GGCC | 1 cut(s) | 690 |
| BtsCI | GGATG | 4 cut(s) | 437, 673, 833, 956 |
| BtsI | GCAGTG | 2 cut(s) | 105, 495 |
| BtsIMutI | CAGTG | 2 cut(s) | 105, 495 |
| Cac8I | GCNNGC | 1 cut(s) | 87 |
| CfoI | GCGC | 4 cut(s) | 87, 89, 563, 982 |
| Cfr13I | GGNCC | 1 cut(s) | 1010 |
| CseI | GACGC | 1 cut(s) | 481 |
| Csp6I | GTAC | 6 cut(s) | 291, 427, 528, 774, 798, 897 |
| CviAII | CATG | 4 cut(s) | 219, 277, 757, 795 |
| CviQI | GTAC | 6 cut(s) | 291, 427, 528, 774, 798, 897 |
| DdeI | CTNAG | 1 cut(s) | 77 |
| DinI | GGCGCC | 1 cut(s) | 562 |
| DpnI | GATC | 6 cut(s) | 50, 68, 242, 546, 849, 866 |
| DpnII | GATC | 6 cut(s) | 48, 66, 240, 544, 847, 864 |
| Eam1104I | CTCTTC | 3 cut(s) | 392, 403, 814 |
| EarI | CTCTTC | 3 cut(s) | 392, 403, 814 |
| Eco130I | CCWWGG | 1 cut(s) | 960 |
| Eco32I | GATATC | 1 cut(s) | 343 |
| Eco47I | GGWCC | 1 cut(s) | 1010 |
| EcoRI | GAATTC | 4 cut(s) | 4, 469, 752, 952 |
| EcoRV | GATATC | 1 cut(s) | 343 |
| EcoT14I | CCWWGG | 1 cut(s) | 960 |
| EgeI | GGCGCC | 1 cut(s) | 562 |
| EheI | GGCGCC | 1 cut(s) | 562 |
| ErhI | CCWWGG | 1 cut(s) | 960 |
| FaeI | CATG | 4 cut(s) | 222, 280, 760, 798 |
| FaqI | GGGAC | 2 cut(s) | 333, 521 |
| FatI | CATG | 4 cut(s) | 218, 276, 756, 794 |
| FauNDI | CATATG | 1 cut(s) | 358 |
| FbaI | TGATCA | 2 cut(s) | 48, 240 |
| Fnu4HI | GCNGC | 3 cut(s) | 118, 609, 994 |
| FokI | GGATG | 4 cut(s) | 444, 680, 820, 943 |
| Fsp4HI | GCNGC | 3 cut(s) | 118, 609, 994 |
| FspBI | CTAG | 3 cut(s) | 321, 525, 971 |
| GlaI | GCGC | 4 cut(s) | 86, 88, 562, 981 |
| GluI | GCNGC | 3 cut(s) | 118, 609, 994 |
| HaeII | RGCGCY | 1 cut(s) | 564 |
| HaeIII | GGCC | 1 cut(s) | 690 |
| HapII | CCGG | 1 cut(s) | 587 |
| HgaI | GACGC | 1 cut(s) | 481 |
| HhaI | GCGC | 4 cut(s) | 87, 89, 563, 982 |
| Hin1I | GRCGYC | 1 cut(s) | 561 |
| Hin1II | CATG | 4 cut(s) | 222, 280, 760, 798 |
| Hin6I | GCGC | 4 cut(s) | 85, 87, 561, 980 |
| HinP1I | GCGC | 4 cut(s) | 85, 87, 561, 980 |
| HindIII | AAGCTT | 1 cut(s) | 933 |
| HinfI | GANTC | 3 cut(s) | 34, 596, 986 |
| HpaII | CCGG | 1 cut(s) | 587 |
| HphI | GGTGA | 3 cut(s) | 804, 844, 901 |
| Hpy166II | GTNNAC | 2 cut(s) | 178, 897 |
| Hpy188I | TCNGA | 5 cut(s) | 266, 411, 844, 917, 931 |
| Hpy188III | TCNNGA | 2 cut(s) | 614, 862 |
| Hpy8I | GTNNAC | 2 cut(s) | 178, 897 |
| HpyAV | CCTTC | 1 cut(s) | 1029 |
| HpyCH4III | ACNGT | 7 cut(s) | 194, 310, 445, 454, 488, 913, 1028 |
| HpyCH4IV | ACGT | 3 cut(s) | 228, 414, 893 |
| HpyCH4V | TGCA | 6 cut(s) | 98, 117, 435, 440, 483, 1002 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 95, 489, 608, 702, 999 |
| HpyF3I | CTNAG | 1 cut(s) | 77 |
| HpySE526I | ACGT | 3 cut(s) | 228, 414, 893 |
| Hsp92I | GRCGYC | 1 cut(s) | 561 |
| Hsp92II | CATG | 4 cut(s) | 222, 280, 760, 798 |
| HspAI | GCGC | 4 cut(s) | 85, 87, 561, 980 |
| KasI | GGCGCC | 1 cut(s) | 560 |
| Ksp22I | TGATCA | 2 cut(s) | 48, 240 |
| Kzo9I | GATC | 6 cut(s) | 48, 66, 240, 544, 847, 864 |
| LguI | GCTCTTC | 1 cut(s) | 814 |
| LmnI | GCTCC | 1 cut(s) | 835 |
| Lsp1109I | GCAGC | 2 cut(s) | 129, 980 |
| LweI | GCATC | 4 cut(s) | 85, 422, 719, 967 |
| MaeI | CTAG | 3 cut(s) | 321, 525, 971 |
| MaeII | ACGT | 3 cut(s) | 228, 414, 893 |
| MaeIII | GTNAC | 6 cut(s) | 214, 445, 454, 493, 550, 907 |
| MalI | GATC | 6 cut(s) | 50, 68, 242, 546, 849, 866 |
| MboI | GATC | 6 cut(s) | 48, 66, 240, 544, 847, 864 |
| MboII | GAAGA | 6 cut(s) | 350, 379, 390, 774, 801, 857 |
| MfeI | CAATTG | 2 cut(s) | 540, 700 |
| MflI | RGATCY | 2 cut(s) | 847, 864 |
| Mly113I | GGCGCC | 1 cut(s) | 561 |
| MmeI | TCCRAC | 3 cut(s) | 894, 940, 1047 |
| MnlI | CCTC | 7 cut(s) | 37, 48, 205, 243, 361, 383, 1025 |
| MspA1I | CMGCKG | 1 cut(s) | 905 |
| MspI | CCGG | 1 cut(s) | 587 |
| MunI | CAATTG | 2 cut(s) | 540, 700 |
| Mva1269I | GAATGC | 1 cut(s) | 273 |
| MvnI | CGCG | 1 cut(s) | 87 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 95, 489, 608, 702, 999 |
| NarI | GGCGCC | 1 cut(s) | 561 |
| NdeI | CATATG | 1 cut(s) | 358 |
| NdeII | GATC | 6 cut(s) | 48, 66, 240, 544, 847, 864 |
| NlaIII | CATG | 4 cut(s) | 222, 280, 760, 798 |
| NlaIV | GGNNCC | 3 cut(s) | 562, 585, 745 |
| NmuCI | GTSAC | 2 cut(s) | 493, 907 |
| NspI | RCATGY | 2 cut(s) | 222, 798 |
| PauI | GCGCGC | 1 cut(s) | 85 |
| PciI | ACATGT | 1 cut(s) | 794 |
| PciSI | GCTCTTC | 1 cut(s) | 814 |
| PctI | GAATGC | 1 cut(s) | 273 |
| PfeI | GAWTC | 3 cut(s) | 34, 596, 986 |
| PkrI | GCNGC | 3 cut(s) | 119, 610, 995 |
| PluTI | GGCGCC | 1 cut(s) | 564 |
| PscI | ACATGT | 1 cut(s) | 794 |
| PspN4I | GGNNCC | 3 cut(s) | 562, 585, 745 |
| PspPI | GGNCC | 1 cut(s) | 1010 |
| PstI | CTGCAG | 1 cut(s) | 119 |
| PsuI | RGATCY | 2 cut(s) | 847, 864 |
| PteI | GCGCGC | 1 cut(s) | 85 |
| PvuII | CAGCTG | 1 cut(s) | 905 |
| RsaI | GTAC | 6 cut(s) | 292, 428, 529, 775, 799, 898 |
| RsaNI | GTAC | 6 cut(s) | 291, 427, 528, 774, 798, 897 |
| SapI | GCTCTTC | 1 cut(s) | 814 |
| SatI | GCNGC | 3 cut(s) | 118, 609, 994 |
| Sau3AI | GATC | 6 cut(s) | 48, 66, 240, 544, 847, 864 |
| Sau96I | GGNCC | 1 cut(s) | 1010 |
| ScaI | AGTACT | 3 cut(s) | 292, 529, 775 |
| SfaNI | GCATC | 4 cut(s) | 85, 422, 719, 967 |
| SfcI | CTRYAG | 2 cut(s) | 115, 627 |
| SfoI | GGCGCC | 1 cut(s) | 562 |
| SinI | GGWCC | 1 cut(s) | 1010 |
| SsiI | CCGC | 2 cut(s) | 600, 608 |
| SspDI | GGCGCC | 1 cut(s) | 560 |
| SspI | AATATT | 1 cut(s) | 286 |
| SspMI | CTAG | 3 cut(s) | 321, 525, 971 |
| StyI | CCWWGG | 1 cut(s) | 960 |
| TaaI | ACNGT | 7 cut(s) | 194, 310, 445, 454, 488, 913, 1028 |
| TaiI | ACGT | 3 cut(s) | 231, 417, 896 |
| TaqI | TCGA | 5 cut(s) | 65, 210, 353, 375, 785 |
| TatI | WGTACW | 4 cut(s) | 290, 527, 773, 797 |
| TauI | GCSGC | 1 cut(s) | 611 |
| TfiI | GAWTC | 3 cut(s) | 34, 596, 986 |
| TscAI | CASTG | 2 cut(s) | 105, 502 |
| TseFI | GTSAC | 2 cut(s) | 493, 907 |
| TseI | GCWGC | 2 cut(s) | 117, 993 |
| Tsp45I | GTSAC | 2 cut(s) | 493, 907 |
| TspDTI | ATGAA | 4 cut(s) | 17, 745, 768, 945 |
| TspGWI | ACGGA | 1 cut(s) | 444 |
| TspRI | CASTG | 2 cut(s) | 105, 502 |
| VpaK11BI | GGWCC | 1 cut(s) | 1010 |
| XapI | RAATTY | 5 cut(s) | 4, 469, 752, 940, 952 |
| XceI | RCATGY | 2 cut(s) | 222, 798 |
| XspI | CTAG | 3 cut(s) | 321, 525, 971 |
| ZrmI | AGTACT | 3 cut(s) | 292, 529, 775 |